Issue Information
(Source: Biotechnology Progress)
Source: Biotechnology Progress - August 25, 2023 Category: Biotechnology Tags: ISSUE INFORMATION Source Type: research
Bioengineered chimeric tRNA/pre ‐miRNAs as prodrugs in cancer therapy
AbstractToday, biologic prodrugs have led to targeting specific tumor markers and have increased specificity and selectivity in cancer therapy. Various studies have shown the role of ncRNAs in cancer pathology and tumorigenesis and have suggested that ncRNAs, especially miRNAs, are valuable molecules in understanding cancer biology and therapeutic processes. Most miRNAs-based research and treatment are limited to chemically synthesized miRNAs. Synthetic alterations in these miRNA mimics may affect their folding, safety profile, and even biological activity. However, despite synthetic miRNA mimics produced by automated syst...
Source: Biotechnology Progress - August 23, 2023 Category: Biotechnology Authors: Fatemeh Rafieenia,
Seyed Omar Ebrahimi,
Ensieh sadat Emadi,
Forough Taheri,
Somayeh Reiisi Tags: REVIEW Source Type: research
Glass microspargers as effective frit spargers in single use bioreactors
AbstractFor multiple-use bench scale and larger bioreactors, sintered stainless steel frit spargers are commonly used as microspargers. For bench-scale single-use bioreactors (SUBs), existing microspargers are sintered plastics, such as polyethylene. However, though plastics are readily sterilized by irradiation making them convenient for single use, these designs overlook surface energy properties of the materials of construction. For these sintered plastic spargers, forces at the water-air-surface interface cause bubble coalescence, leading to lower effective mass transfer, higher gas flow rates, and differing pCO2 profi...
Source: Biotechnology Progress - August 9, 2023 Category: Biotechnology Authors: William Tran,
T. Craig Seamans,
John S. Bowers Tags: RESEARCH ARTICLE Source Type: research
Isoelectric point (pI) ‐based phase separation (pI‐BPS) purification of elastin‐like polypeptides (ELPs) containing charged, biologically active fusion proteins (ELP‐FPs)
We report a dramatic increase in protein yield when using pI-BPS for purification of ELP-FPs. Proteins purified by this method also retain the functional activity of the protein present in the ELP-FP. Techniques developed here enable significant diversification of possible fusion proteins delivered by ELPs as ELP-FPs by allowing them to be produced and purified at higher quantities and yields. (Source: Biotechnology Progress)
Source: Biotechnology Progress - August 3, 2023 Category: Biotechnology Authors: Truman J. Roland,
Graham L. Strauss,
Nabila Bushra,
Martin Muschol,
Piyush Koria Tags: RESEARCH ARTICLE Source Type: research
Efficient optimization of time ‐varying inputs in a fed‐batch cell culture process using design of dynamic experiments
AbstractIn cell culture process development, we rely largely on an iterative, one-factor-at-a-time procedure based on experiments that explore a limited process space. Design of experiments (DoE) addresses this issue by allowing us to analyze the effects of process inputs on process responses systematically and efficiently. However, DoE cannot be applied directly to study time-varying process inputs unless an impractically large number of bioreactors is used. Here, we adopt the methodology of design of dynamic experiments (DoDE) and incorporate dynamic feeding profiles efficiently in late-stage process development of the m...
Source: Biotechnology Progress - August 2, 2023 Category: Biotechnology Authors: Yu Luo,
Duane A. Stanton,
Rachel C. Sharp,
Alexis J. Parrillo,
Kelsey T. Morgan,
Diana B. Ritz,
Sameer Talwar Tags: RESEARCH ARTICLE Source Type: research
Improved endotoxin removal using ecofriendly detergents for intensified plasmid capture
AbstractIncreasing plasmid demand for both production of viral and gene therapies as well as nucleic acid based vaccines has highlighted bottlenecks in production. One bottleneck is traditional bead-based chromatography as a capture step. To meet the needs of fast-growing markets, new production solutions are needed. These solutions must enable efficient capture of a diverse range of plasmid types and excellent clearance of bacterial host impurities, such as endotoxin. Enhanced endotoxin clearance during chromatographic purification has previously been demonstrated with detergents such as Triton ™ X-100. However, degrada...
Source: Biotechnology Progress - August 2, 2023 Category: Biotechnology Authors: Andr é Kiesewetter,
Akshat Gupta,
Anja Heinen‐Kreuzig,
Trish Greenhalgh,
Andreas Stein Tags: RESEARCH ARTICLE Source Type: research
Sources and methods of manufacturing xanthan by fermentation of various carbon sources
The objective of this analysis is to investigate how utilizing different raw materials can improve the cost-efficient production of xanthan gum. (Source: Biotechnology Progress)
Source: Biotechnology Progress - August 1, 2023 Category: Biotechnology Authors: Rahul Dey,
Biswa Prasun Chatterji Tags: REVIEW Source Type: research
Trace metal optimization in CHO cell culture through statistical design of experiments
AbstractA majority of the biotherapeutics industry today relies on the manufacturing of monoclonal antibodies from Chinese hamster ovary (CHO) cells, yet challenges remain with maintaining consistent product quality from high-producing cell lines. Previous studies report the impact of individual trace metal supplemental on CHO cells, and thus, the combinatorial effects of these metals could be leveraged to improve bioprocesses further. A three-level factorial experimental design was performed in fed-batch shake flasks to evaluate the impact of time wise addition of individual or combined trace metals (zinc and copper) on C...
Source: Biotechnology Progress - July 28, 2023 Category: Biotechnology Authors: Ashli Polanco,
George Liang,
SoYoung Park,
Yongdan Wang,
Ryan J. Graham,
Seongkyu Yoon Tags: RESEARCH ARTICLE Source Type: research
