Removal of chromatin by salt ‐tolerant endonucleases for production of recombinant measles virus
AbstractHost cell DNA is a critical impurity in downstream processing of enveloped viruses. Especially, DNA in the form of chromatin is often neglected. Endonuclease treatment is an almost mandatory step in manufacturing of viral vaccines. In order to find the optimal performer, four different endonucleases, two of them salt tolerant, were evaluated in downstream processing of recombinant measles virus. Endonuclease treatment was performed under optimal temperature conditions after clarification and before the purification by flow-through chromatography with a core shell chromatography medium: Capto ™ Core 700. Virus inf...
Source: Biotechnology Progress - March 28, 2023 Category: Biotechnology Authors: Viktoria Mayer,
Anna ‐Carina Frank,
Shirin Preinsperger,
Patrick Csar,
Petra Steppert,
Alois Jungbauer,
Patricia Pereira Aguilar Tags: RESEARCH ARTICLE Source Type: research
Continuous CTC separation through a DEP ‐based contraction–expansion inertial microfluidic channel
AbstractThe efficient isolation of viable and intact circulating tumor cells (CTCs) from blood is critical for the genetic analysis of cancer cells, prediction of cancer progression, development of drugs, and evaluation of therapeutic treatments. While conventional cell separation devices utilize the size difference between CTCs and other blood cells, they fail to separate CTCs from white blood cells (WBCs) due to significant size overlap. To overcome this issue, we present a novel approach that combines curved contraction –expansion (CE) channels with dielectrophoresis (DEP) and inertial microfluidics to isolate CTCs fr...
Source: Biotechnology Progress - March 27, 2023 Category: Biotechnology Authors: Md Sadiqul Islam,
Xiaolin Chen Tags: RESEARCH ARTICLE Source Type: research
Modulating and optimizing Pluronic F ‐68 concentrations and feeding for intensified perfusion Chinese hamster ovary cell cultures
This study systematically inves tigated the protecting role of PF-68 in intensified CHO cell cultures, shedding a light on the optimization of perfusion cultures through the control of protective additives. (Source: Biotechnology Progress)
Source: Biotechnology Progress - March 27, 2023 Category: Biotechnology Authors: Zhaohui Wei,
Yang Xia,
Yuning Su,
Yufen Quan,
Liuliu Sun,
Shanshan Wang,
Fangjian Zhu,
Zhenzhen Chen,
Jun Tian,
Wei ‐Chun Wang,
Weichang Zhou,
Haiyang Yu Tags: RESEARCH ARTICLE Source Type: research
Multimodal peptide ligand extracts parvovirus from interface in affinity aqueous two ‐phase system
AbstractAqueous two-phase systems (ATPS) have found various applications in bioseparations and microencapsulation. The primary goal of this technique is to partition target biomolecules in a preferred phase, rich in one of the phase-forming components. However, there is a lack of understanding of biomolecule behavior at the interface between the two phases. Biomolecule partitioning behavior is studied using tie-lines (TL), where each TL is a group of systems at thermodynamic equilibrium. Across a TL, a system can either have a bulk PEG-rich phase with citrate-rich droplets, or the opposite can occur. We found that porcine ...
Source: Biotechnology Progress - March 20, 2023 Category: Biotechnology Authors: Pratik U. Joshi,
Stephanie M. Kroger,
Silviya P. Zustiak,
Caryn L. Heldt Tags: RESEARCH ARTICLE Source Type: research
Impact of X ‐ray irradiation as an equivalent alternative to gamma for sterilization of single‐use bioprocessing polymers
AbstractIrradiation sterilization of polymeric pharmaceutical processing systems and medical devices, an essential healthcare technology, is facing critical business continuity challenges, driving the need to qualify equivalent alternative irradiation technologies, such as X-ray. Whereas the underlying there is a paucity of cross-industry published data evaluating X-ray irradiation effects on plastics as compared to gamma irradiation. That leads to regulatory uncertainty in the levels of costly validation data regulators will require and overall apprehension in the rate of X-ray irradiation adoption. The present study eval...
Source: Biotechnology Progress - March 20, 2023 Category: Biotechnology Authors: Adam W. Grzelak,
Sarah Jeffkins,
Lan Luo,
James Stilwell,
James Hathcock Tags: RESEARCH ARTICLE Source Type: research
Evaluating multiproduct chromatography Protein A resin reuse for monoclonal antibodies in biopharmaceutical manufacturing
In this study, we follow a resin lifetime methodology typically used for commercial submissions and apply it to determine the feasibility of purifying different products on a Protein A MabSelect PrismA ™ resin. Three distinct monoclonal antibodies were used as model molecules. Column performance was monitored through chromatogram profiles, yield, clearance capability of selected media components, pressure and product quality. A protein carryover study was designed to demonstrate that the column cleaning procedures reduced protein carryover to safe cleanliness levels regardless of multiple product contact cycles and the o...
Source: Biotechnology Progress - March 14, 2023 Category: Biotechnology Authors: Nivetita Ravi,
Javier Huerta,
Gisela Ferreira Tags: REVIEW Source Type: research
Combining regulated and constitutive protein expression significantly boosts protein expression by increasing productivity without affecting CHO cell growth
In this study, combinations of regulated and constitutive expression systems were used to supertransfect targeted integration (TI) cell lines expressing the same antibody either constitutively or under-regulated expression. Clone screening with a hybrid expression system (inducible + constitutive) allowed identification and selection of higher titer clones under uninduced conditions, without a negative impact on cell growth during clone selection and expansion. Induction of the regulated promoter(s) during the production phase increased the Qp without negatively affecting growth, resulting in approximately twofold higher t...
Source: Biotechnology Progress - March 11, 2023 Category: Biotechnology Authors: Cynthia Lam,
Joseph Carver,
Domingos Ng,
Dejin Zhan,
Danming Tang,
Thara Kandamkalam,
Brad Snedecor,
Gavin Barnard,
Amy Shen,
Shahram Misaghi Tags: RESEARCH ARTICLE Source Type: research
Multimodal Peptide Ligand Extracts Parvovirus from Interface in Affinity Aqueous Two ‐Phase System
This article is protected by copyright. All rights reserved. (Source: Biotechnology Progress)
Source: Biotechnology Progress - March 10, 2023 Category: Biotechnology Authors: Pratik U. Joshi,
Stephanie M. Kroger,
Silviya P. Zustiak,
Caryn L. Heldt Tags: RESEARCH ARTICLE Source Type: research
Use of scanning electron microscopy and energy dispersive X ‐ray spectroscopy to identify key fouling species during alternating tangential filtration
This study used scanning electron microscopy and energy dispersive X-ray spectroscopy to identify the nature and location of foulants on 0.2 μm polyethersulfone hollow fiber membranes after use in industrial Chinese hamster ovary cell perfusion bioreactors for monoclonal antibody production. Membrane fouling was dominated by proteinaceous material, primarily host cell proteins along with some monoclonal antibody. Fouling occurred prim arily on the lumen surface with much less protein trapped within the depth of the fiber. Protein deposition was also most pronounced near the inlet/exit of the hollow fibers, which are the...
Source: Biotechnology Progress - March 8, 2023 Category: Biotechnology Authors: Vikram Sundar,
Da Zhang,
Xianghong Qian,
S. Ranil Wickramasinghe,
John Paul Smelko,
Christina Carbrello,
Yara Jabbour Al Maalouf,
Andrew L. Zydney Tags: RESEARCH ARTICLE Source Type: research
