Molecular characterization of ANKRD1 in rhabdomyosarcoma cell lines: expression, localization, and proteasomal degradation
In this study, we used three RMS cell lines (SJRH30, RD, and HS-729) to assess its expression profile, intracellular localization, and turnover. They express wild-type ANKRD1, as judged by the sequencing of the open reading frame. Each cell line expressed a different amount of ANKRD1 protein, although the transcript level was similar. According to western blot analysis, ANKRD1 protein was expressed at detectable levels in the SJRH30 and RD cells (SJRH30 > RD), but not in the HS-729, even after immunoprecipitation. Immunocytochemistry revealed nuclear and cytoplasmic localization of ANKRD1 in all examined cell line...
Source: Histochemistry and Cell Biology - February 23, 2024 Category: Biomedical Science Source Type: research
Evaluation of TRIM63 RNA in situ hybridization (RNA-ISH) as a potential biomarker for alveolar soft-part sarcoma (ASPS)
AbstractAlveolar soft-part sarcoma (ASPS) is a rare soft tissue tumor with a broad morphologic differential diagnosis. While histology and immunohistochemistry can be suggestive, diagnosis often requires exclusion of other entities followed by confirmatory molecular analysis for its characteristicASPSCR1-TFE3 fusion. Current stain-based biomarkers (such as immunohistochemistry for cathepsin K and TFE3) show relatively high sensitivity but may lack specificity, often showing staining in multiple other entities under diagnostic consideration. Given the discovery of RNA in situ hybridization (RNA-ISH) forTRIM63 as a sensitive...
Source: Medical Oncology - February 23, 2024 Category: Cancer & Oncology Source Type: research
Cancers, Vol. 16, Pages 853: Statin-Sensitive Akt1/Src/Caveolin-1 Signaling Enhances Oxidative Stress Resistance in Rhabdomyosarcoma
o Sampaolesi
Stefano Gastaldello
Matteo Cassandri
Francesco Marampon
Alessandro Fanzani
Identifying the molecular mechanisms underlying radioresistance is a priority for the treatment of RMS, a myogenic tumor accounting for approximately 50% of all pediatric soft tissue sarcomas. We found that irradiation (IR) transiently increased phosphorylation of Akt1, Src, and Cav1 in human RD and RH30 lines. Synthetic inhibition of Akt1 and Src phosphorylation increased ROS levels in all RMS lines, promoting cellular radiosensitization. Accordingly, the elevated activation of the Akt1/Src/Cav1 pathway, as detected in two ...
Source: Cancers - February 20, 2024 Category: Cancer & Oncology Authors: Silvia Codenotti Leonardo Sandrini Delia Mandracchia Luisa Lorenzi Giovanni Corsetti Maura Poli Michela Asperti Valentina Salvi Daniela Bosisio Eugenio Monti Stefania Mitola Luca Triggiani Michele Guescini Enrico Pozzo Maurilio Sampaolesi Stefano Gastalde Tags: Article Source Type: research
Evaluation of clinical and imaging features for differentiating rhabdomyosarcoma from neuroblastoma in pediatric soft tissue
ConclusionThe clinical and imaging features-based nomogram has potential for helping radiologists distinguish between pediatric soft tissue RMS and NB patients preoperatively, and reduce unnecessary interventions. (Source: Frontiers in Oncology)
Source: Frontiers in Oncology - February 9, 2024 Category: Cancer & Oncology Source Type: research
GSE240722 CD73 contributes to the pathogenesis of Fusion-Negative Rhabdomyosarcoma through the purinergic signaling pathway
Contributors : Lei Guo ; Lin Xu ; Akansha M Shah ; Victor A Lopez ; Vincent S Tagliabracci ; Kenian Chen ; Lin Xu ; Rhonda Bassel-Duby ; Eric N Olson ; Ning LiuSeries Type : Expression profiling by high throughput sequencingOrganism :We performed RNA-seq to evaluated the transcriptional changes of CD73 knockdown in RD cells, a Fusion Negative Rhabdomyosarcoma cell line. We used a Dox-inducible shRNA targetting the 3'UTR of CD73 to knockdown this gene. Cells with No-Dox were used as control. (Source: GEO: Gene Expression Omnibus)
Source: GEO: Gene Expression Omnibus - February 7, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Source Type: research
Contemporary preclinical mouse models for pediatric rhabdomyosarcoma: from bedside to bench to bedside
ConclusionsThis up-to-date review highlights the need for mouse models with dissemination phenotypes and cell lines from primary untreated tumors. Furthermore, efforts should be directed towards underexplored areas such as surgery, radiotherapy, and biomarkers. (Source: Frontiers in Oncology)
Source: Frontiers in Oncology - February 2, 2024 Category: Cancer & Oncology Source Type: research
High-dose chemotherapy for Ewing Sarcoma and Rhabdomyosarcoma: a systematic review by the Australia and New Zealand Sarcoma Association clinical practice guidelines working party
Ewing sarcoma (ES) and rhabdomyosarcoma (RMS) are small round blue cell tumours that occur in children, adolescents and young adults (AYA) [1 –3]. In ES, the EWSR1-FLI1 fusion is identified in 85% of cases [1,4]. Small round cell sarcomas of bone and soft tissue where EWSR1-FLI1 fusion is not identified were previously collated with ES and termed the Ewing sarcoma family of tumours (ESFT) [1]. Molecular profiling has revealed fusions in cluding EWSR1–non-ETS fusions, CIC-rearrangements, and BCOR alterations by which these tumours are now classified and understood to confer different natural histories and treatment sens...
Source: Cancer Treatment Reviews - February 1, 2024 Category: Cancer & Oncology Authors: Ashika Ramamurthy, Elizabeth A Connolly, Jasmine Mar, Jeremy Lewin, Vivek A Bhadri, Marianne B Phillips, Mark Winstanley, Lisa M Orme, Peter Grimison, Joanna Connor, Smaro Lazarakis, Angela M Hong, Natacha Omer, Julie Cayrol Source Type: research
Application of hysteroscopy and vaginoscopy in follow up of young patients with genital tract rhabdomyosarcoma
Objective: Does operative hysteroscopy and vaginoscopy can be applied in detection of relapse and as a follow up of young patients with RMS of genital tract. (Source: European Journal of Obstetrics, Gynecology, and Reproductive Biology)
Source: European Journal of Obstetrics, Gynecology, and Reproductive Biology - February 1, 2024 Category: OBGYN Authors: Patrycja Gumu ła, Katarzyna Sachadel, Ewa Filipp, Anna Raciborska, Tadeusz Issat Source Type: research
Case report of 13-year old girl with rms of the uterus- gynecological intervention and oncolfertility procedure
Introduction: This is a case report of a 13 year old girl diagnosed with rhabdomyosarcoma embryonale of the uterus. After the initial chemotherapy there was an abnormal tissue left in the anterior wall of the uterus in MRI report. The patient was qualified for two step diagnostic approach to detectany residual neoplasia. First was operative hysteroscopy and the second was diagnostic laparoscopy combined with oncofertility procedure. (Source: European Journal of Obstetrics, Gynecology, and Reproductive Biology)
Source: European Journal of Obstetrics, Gynecology, and Reproductive Biology - February 1, 2024 Category: OBGYN Authors: Katarzyna Sachadel, Patrycja Gumu ła, Aleksandra Januszaniec, Anna Raciborska, Monika Bekesińska-Figatowska Source Type: research
Ocular and Orbital Tumors in Childhood
Pediatric tumors of the eye and orbit can be benign or malignant as well as congenital or acquired and are usually distinctively different than those seen in adults.1 Although most of these neoplasms are benign (e.g. dermoid cyst, chalazion or molluscum), their location near and within a vital organ can result in serious dermatologic and ophthalmologic sequelae. Lesions discussed include vascular lesions, retinoblastomas (the most common primary pediatric intraocular malignancy), rhabdomyosarcoma (the most common primary pediatric orbital malignancy), Langerhans cell histiocytosis, and metastatic lesions to the orbit (neur...
Source: Clinics in Dermatology - January 30, 2024 Category: Dermatology Authors: Kathryn Bentivegna, Nicholas J. Saba, Roman Shinder, Jane M. Grant-Kels Source Type: research
When the second comes first – rhabdomyosarcoma preceding heritable retinoblastoma– a case report
Retinoblastoma (rb) is the most frequent intraocular tumor, accounting for 3% of all childhood cancers. Heritable rb survivors are germline carriers for an RB1 mutation and have a lifelong risk to develop non-... (Source: BMC Ophthalmology)
Source: BMC Ophthalmology - January 30, 2024 Category: Opthalmology Authors: Devjyoti Tripathy, Alexandre Moulin, Jacques Bijon, Carole Gengler, Maja Beck-Popovic, Francis L. Munier and Christina Stathopoulos Tags: Case Report Source Type: research
Resection of a Large Growing Mediastinal Germ Cell Tumor Using a Multidisciplinary Approach
Curr Oncol. 2023 Dec 21;31(1):42-49. doi: 10.3390/curroncol31010003.ABSTRACTMediastinal germ cell tumors (GCTs) are rare. Post-chemotherapy residual masses in patients with a nonseminomatous GCT require resection. A patient with a large mediastinal GCT involving the left subclavian artery, superior vena cava (SVC) and hilum of the right lung is presented. Despite a biochemical response to chemotherapy, the tumor enlarged on serial imaging. With guidance from medical oncology, a multidisciplinary surgical team, including cardiac anesthesia, cardiac surgery and thoracic surgery resected the tumor with a staged reconstruction...
Source: Current Oncology - January 26, 2024 Category: Cancer & Oncology Authors: Alison Greene Lori Wood Philip Champion Mathieu Castonguay Matthias Scheffler Catherine Deshaies Jeremy Wood Daniel French Source Type: research
