Volume electron microscopy: analyzing the lung
AbstractSince its entry into biomedical research in the first half of the twentieth century, electron microscopy has been a valuable tool for lung researchers to explore the lung ’s delicate ultrastructure. Among others, it proved the existence of a continuous alveolar epithelium and demonstrated the surfactant lining layer. With the establishment of serial sectioning transmission electron microscopy, as the first “volume electron microscopic” technique, electron micro scopy entered the third dimension and investigations of the lung’s three-dimensional ultrastructure became possible. Over the years,...
Source: Histochemistry and Cell Biology - September 17, 2020 Category: Biomedical Science Source Type: research

In focus in HCB
(Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - September 16, 2020 Category: Biomedical Science Source Type: research

Potential of zebrafish as a model to characterise MicroRNA profiles in mechanically mediated joint degeneration
AbstractMechanically mediated joint degeneration and cartilage dyshomeostasis is implicated in highly prevalent diseases such as osteoarthritis. Increasingly, MicroRNAs are being associated with maintaining the normal state of cartilage, making them an exciting and potentially key contributor to joint health and disease onset. Here, we present a summary of current in vitro and in vivo models which can be used to study the role of mechanical load and MicroRNAs in joint degeneration, including: non-invasive murine models of PTOA, surgical models which involve ligament transection, and unloading models based around immobilisa...
Source: Histochemistry and Cell Biology - September 15, 2020 Category: Biomedical Science Source Type: research

Posttranscriptional regulation of maternal Pou5f1/Oct4 during mouse oogenesis and early embryogenesis
AbstractProtein syntheses at appropriate timings are important for promoting diverse biological processes and are controlled at the levels of transcription and translation. Pou5f1/Oct4 is a transcription factor that is essential for vertebrate embryonic development. However, the precise timings when the mRNA and protein ofPou5f1/Oct4 are expressed during oogenesis and early stages of embryogenesis remain unclear. We analyzed the expression patterns of mRNA and protein ofPou5f1/Oct4 in mouse oocytes and embryos by using a highly sensitive in situ hybridization method and a monoclonal antibody specific to Pou5f1/Oct4, respec...
Source: Histochemistry and Cell Biology - September 15, 2020 Category: Biomedical Science Source Type: research

Zebrafish cardiac regeneration —looking beyond cardiomyocytes to a complex microenvironment
AbstractThe study of heart repair post-myocardial infarction has historically focused on the importance of cardiomyocyte proliferation as the major factor limiting adult mammalian heart regeneration. However, there is mounting evidence that a narrow focus on this one cell type discounts the importance of a complex cascade of cell –cell communication involving a whole host of different cell types. A major difficulty in the study of heart regeneration is the rarity of this process in adult animals, meaning a mammalian template for how this can be achieved is lacking. Here, we review the adult zebrafish as an ideal and ...
Source: Histochemistry and Cell Biology - September 14, 2020 Category: Biomedical Science Source Type: research

Zebrafish as a model to study autophagy and its role in skeletal development and disease
AbstractIn the last twenty years, research using zebrafish as a model organism has increased immensely. With the many advantages that zebrafish offer such as high fecundity, optical transparency, ex vivo development, and genetic tractability, they are well suited to studying developmental processes and the effect of genetic mutations. More recently, zebrafish models have been used to study autophagy. This important protein degradation pathway is needed for cell and tissue homeostasis in a variety of contexts. Correspondingly, its dysregulation has been implicated in multiple diseases including skeletal disorders. In this r...
Source: Histochemistry and Cell Biology - September 11, 2020 Category: Biomedical Science Source Type: research

The plate body: 3D ultrastructure of a facultative organelle of alveolar epithelial type II cells involved in SP-A trafficking
In conclusion, the present study clearly proves the connection of plate bodies with the rough endoplasmic reticulum and the presence of a vesicular network as part of the organelle involved in SP-A trafficking. (Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - September 2, 2020 Category: Biomedical Science Source Type: research

The role of EP-2 receptor expression in cervical intraepithelial neoplasia
AbstractProstaglandin induced signalling is involved in different cancers. As previously described, the EP3 receptor expression decreases with increasing stage of cervical intraepithelial lesions (CIN). In addition, in cervical cancer EP3 is an independent prognosticator for overall survival and correlates with FIGO stages. Currently the role of Prostaglandin 2 receptor 2 (EP2) in CIN is unknown. The aim of this study was to analyse the expression of EP2 for potential prognostic value for patients with cervical dysplasia. EP2 expression was analysed by immunohistochemistry in 33 patient samples (CIN1 –3) using the im...
Source: Histochemistry and Cell Biology - August 26, 2020 Category: Biomedical Science Source Type: research

Remodeling of the skeletal muscle and postsynaptic component after short-term joint immobilization and aquatic training
AbstractJoint immobilization is commonly used as a conservative treatment for osteoarticular and musculotendinous traumas. However, joint immobilization might elicit degenerative effects on the neuromuscular system and muscle atrophy. For this reason, the choice of strategies that mitigate these effects is essential in the post-immobilization period. Therefore, this study aimed to investigate the impact of aquatic training on the morphology of muscle fibers and motor endplates of the gastrocnemius muscle in the post-immobilization period. Male Wistar rats (90  days old) were divided into groups: Sedentary: no procedur...
Source: Histochemistry and Cell Biology - August 14, 2020 Category: Biomedical Science Source Type: research

Retraction Note to: Explore on the effect of ATF6 on cell growth and apoptosis in cartilage development
The Editor-in-Chief has retracted the article by Han et al. (2014) because Fig.  3a–d are also published as Fig. 5b–e in Liu et al. (2012), and Fig. 3a, c, d are also published as Fig. 5a, d, e in Guo et al. (2014). (Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - August 10, 2020 Category: Biomedical Science Source Type: research

Low fluid shear stress promoted ciliogenesis via Dvl2 in hUVECs
This study aims to explore the mechanism of fluid shear stress in regulating the primary cilia assembly or disassembly in human umbilical vein endothelial cells (hUVECs) using microfluidic chamber experiments. Immunofluorescence analysis showed that primary cilia assembled under disturbed fluid shear stress (DF) of 1  dyne/cm2, while disassembled under unidirectional shear stress (USS) of 15  dynes/cm2. Disheveled (Dvl2) in Wnt signaling pathway was effectively co-immunoprecipitated with Bardet –Biedl syndrome proteins 8 (Bbs8) and γ-tubulin. Compared with those in the control group, the percentages o...
Source: Histochemistry and Cell Biology - August 9, 2020 Category: Biomedical Science Source Type: research

Critical assessment of staining properties of a new visualization technology: a novel, rapid and powerful immunohistochemical detection approach
AbstractImmunohistochemical staining of tissue sections is a vital technique in pathological diagnostics and theranostics. Several kinds of detection systems are available —each of them with their advantages and disadvantages. Here we present the results of a study assessing a prototype immunohistochemical detection technology (PIDT) for visualization of antigens in tissue sections. Different tumor tissues (n = 11) were stained with selected antibodies (n = 30) and a subset of these under different fixation conditions. The staining properties were assessed according to six staining quality...
Source: Histochemistry and Cell Biology - August 7, 2020 Category: Biomedical Science Source Type: research

In focus in HCB
(Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - July 29, 2020 Category: Biomedical Science Source Type: research

Spatio-temporal distribution of tubulin-binding cofactors and posttranslational modifications of tubulin in the cochlea of mice
AbstractThe five tubulin-binding cofactors (TBC) are involved in tubulin synthesis and the formation of microtubules. Their importance is highlighted by various diseases and syndromes caused by dysfunction or mutation of these proteins. Posttranslational modifications (PTMs) of tubulin promote different characteristics, including stability-creating subpopulations of tubulin. Cell- and time-specific distribution of PTMs has only been investigated in the organ of Corti in gerbils. The aim of the presented study was to investigate the cell type-specific and time-specific expression patterns of TBC proteins and PTMs for the fi...
Source: Histochemistry and Cell Biology - July 25, 2020 Category: Biomedical Science Source Type: research

High endogenous expression of parathyroid hormone-related protein (PTHrP) supports osteogenic differentiation in human dental follicle cells
In conclusion, our data suggest that a high endogenous expression of PTHrP in DFCs supports the induction of osteogenic differentiation via an intracrine mode. (Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - July 24, 2020 Category: Biomedical Science Source Type: research

Molecular differences between arterial and venous grafts in the first year after coronary artery bypass grafting
AbstractDespite commonly used for coronary artery bypass surgery, saphenous vein (SV) grafts have significantly lower patency rates in comparison to internal thoracic artery (ITA) grafts, which might be due to the structural characteristics of the vessel wall but also due to differences in oxidative stress adaptation and molecular signaling and regulation. This human post mortem study included a total of 150 human bypass grafts (75 SV grafts and 75 ITA grafts) obtained from 60 patients divided into five groups due to the time period of implantation: group 1: baseline group without grafting; group 2: 1  day; group 3:&t...
Source: Histochemistry and Cell Biology - July 23, 2020 Category: Biomedical Science Source Type: research

Imaging lung regeneration by light sheet microscopy
This study identifies regional stem cell niches as starting points for epithelial recovery, underscores the enormous regenerative capacity of the respiratory epithelium and demonstrates the power of whole lung 3D imaging f or evaluating the extent of pulmonary damage and subsequent repair processes. (Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - July 19, 2020 Category: Biomedical Science Source Type: research

Histological validation of adipogenic differentiation potential of ASC on collagen-based 2D scaffolds
AbstractDetermination of the adipogenic potential and behavior of adipose-derived mesenchymal stem/stromal cells (ASCs) is particularly relevant for their potential clinical application in regenerative medicine, especially when regeneration is supported by biomaterials or scaffolds. Scaffolds need to be able to induce tissue repair and limit undesired adipogenic differentiation. Depending on the scaffold employed, determination of cell behavior may be hindered by material interference with staining, which will limit either cells identification or dye quantification. Collagen is a promising biomaterial in regenerative medic...
Source: Histochemistry and Cell Biology - July 14, 2020 Category: Biomedical Science Source Type: research

Cloning and expression profiling of muscle regulator ANKRD2 in domestic chicken Gallus gallus
In this study, we report on characterization of chickenANKRD2. The chickenANKRD2 coding region contains 1002  bp and encodes a 334-amino acid protein which shares approximately 58% identity with human and mouse orthologs, mostly in the conserved region of ankyrin repeats. Comprehensive analysis of theANKRD2 gene and protein expression in adult chicken demonstrated its predominant expression in red muscles of thigh and drumstick, compared to white muscle. It was not detected in heart and white pectoral muscle. Uneven expression of ANKRD2 in chicken skeletal muscles, observed by immunohistochemistry, was attributed to i...
Source: Histochemistry and Cell Biology - July 11, 2020 Category: Biomedical Science Source Type: research

Global changes in epigenomes during mouse spermatogenesis: possible relation to germ cell apoptosis
AbstractMammalian spermatogenesis is characterized by disproportionate germ cell apoptosis. The high frequency of apoptosis is considered a safety mechanism that serves to avoid unfavorable transmission of paternal aberrant genetic information to the offspring as well as elimination mechanism for removal of overproduced immature or damaged spermatogenic cells. The molecular mechanisms involved in the induction of germ cell apoptosis include both intrinsic mitochondrial Bcl-2/Bax and extrinsic Fas/FasL pathways. However, little is known about the nuclear trigger of those systems. Recent studies indicate that epigenomes are ...
Source: Histochemistry and Cell Biology - July 11, 2020 Category: Biomedical Science Source Type: research

Divergence exists in the subcellular distribution of intramuscular triglyceride in human skeletal muscle dependent on the choice of lipid dye
AbstractDespite over 50  years of research, a comprehensive understanding of how intramuscular triglyceride (IMTG) is stored in skeletal muscle and its contribution as a fuel during exercise is lacking. Immunohistochemical techniques provide information on IMTG content and lipid droplet (LD) morphology on a fibre type and subcellular-specific basis, and the lipid dye Oil Red O (ORO) is commonly used to achieve this. BODIPY 493/503 (BODIPY) is an alternative lipid dye with lower background staining and narrower emission spectra. Here we provide the first quantitative comparison of BODIPY and ORO for investigating exe r...
Source: Histochemistry and Cell Biology - July 5, 2020 Category: Biomedical Science Source Type: research

Unloading during skeletal muscle regeneration retards iNOS-expressing macrophage recruitment and perturbs satellite cell accumulation
This study focused on macrophages expressing inducible nitric oxide synthase (iNOS) that synthesize nitric oxide, a key regulator of muscle regeneration, and compared the continuous hindlimb unloading (HU) by tail suspensionversus weight-bearing (WB) after skeletal muscle crush injury in rats. We found that in the WB group, the recruitment of iNOS+ proinflammatory macrophages into the injured site gradually increased until their peak number at 48  h post-injury. In the HU group, the accumulation of iNOS+ macrophages until 48  h after injury was significantly less than that in the WB group and continued to increas...
Source: Histochemistry and Cell Biology - July 3, 2020 Category: Biomedical Science Source Type: research

In focus in HCB
(Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - June 30, 2020 Category: Biomedical Science Source Type: research

Two new reliable immunohistochemical methods for simultaneous identification of capillaries, the three types of fibers and basal lamina in human skeletal muscle
This article proposes two time- and tissue-sparing immunohistochemical methods to accurately assess a complete fiber typing (type I, IIA, and IIX) along with muscle capilla rization on a single muscle section. (Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - June 26, 2020 Category: Biomedical Science Source Type: research

Cytoplasmic VDR expression as an independent risk factor for ovarian cancer
AbstractThe vitamin D receptor (VDR), primarily known as a crucial mediator of calcium homeostasis and metabolism, has been shown to play a significant role in various cancer entities. Previous studies have focused on vitamin D and its receptor in gynecological cancers, noting that the receptor is upregulated in epithelial ovarian cancer (EOC). The aim of this study is to analyze the prognostic impact of VDR and its functional significance in ovarian cancer. Through immunohistochemistry, VDR staining was examined in 156 ovarian cancer samples. Evaluation of VDR staining was conducted in the nucleus and the cytoplasm using ...
Source: Histochemistry and Cell Biology - June 22, 2020 Category: Biomedical Science Source Type: research

Determining factors for optimal neuronal and glial Golgi-Cox staining
AbstractGolgi staining allows for the analysis of neuronal arborisations and connections and is considered a powerful tool in basic and clinical neuroscience. The fundamental rules for improving neuronal staining using theGolgi-Cox method are not fully understood; both intrinsic and extrinsic factors may control the staining process. Therefore, various conditions were tested to improve theGolgi-Cox protocol for vibratome-cut rat brain sections. Optimal staining of cortical neurons was achieved after 72  h of impregnation. Well-stained neurons in both cortical and subcortical structures were observed after 96 h of...
Source: Histochemistry and Cell Biology - June 12, 2020 Category: Biomedical Science Source Type: research

AT-rich interactive domain 1A protein expression in normal and pathological pregnancies complicated by preeclampsia
AbstractAT-rich interactive domain 1A (ARID1A, as known as BAF250a) is a subunit of human switch/sucrose nonfermentable chromatin remodeling complex with tumour suppressor function. Mutations of Arid1a have been reported in many human cancers and low expression of this protein has been correlated to a poor prognosis outcome in patients affected by some types of cancer. Although there are many studies regarding ARID1A functions in cancer, little is known about its role in regulating cell differentiation and normal tissues homeostasis. Here, we investigate ARID1A expression in normal placental tissues of first and third trim...
Source: Histochemistry and Cell Biology - June 11, 2020 Category: Biomedical Science Source Type: research

Correction to: Influence of protein (human galectin-3) design on aspects of lectin activity
In the original publication of the article, the “Result” section has been published (Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - June 9, 2020 Category: Biomedical Science Source Type: research

The loss of global DNA methylation due to decreased DNMT expression in the postnatal mouse ovaries may associate with infertility emerging during ovarian aging
AbstractOvarian aging is one of the main causes of female infertility, and its molecular background is still largely unknown. As DNA methylation regulates many oogenesis/folliculogenesis-related genes, the expression levels and cellular localizations of DNA methyltransferases (DNMTs) playing key roles in this process is important in the ovaries from early to aged terms. In the present study, we aimed to evaluate the spatial and temporal expression of theDnmt1,Dnmt3a,Dnmt3b, andDnmt3l genes as well as global DNA methylation levels in the mouse ovaries during aging. For this purpose, the following groups were created: young ...
Source: Histochemistry and Cell Biology - June 8, 2020 Category: Biomedical Science Source Type: research

Histochemical detection of free thiols in glandular cells and tissues of different marine Polychaeta
AbstractEither through differentiated glands or specialised individual cells, the coating epithelia of soft-bodied marine invertebrates are responsible for the secretion of a broad span of peptidic substances, from protective mucins to biocides. These secretions are characterised by the presence of cysteine-rich proteins and peptides, rendering a distinct histochemical signature of secretory epithelia. Through a histochemical procedure for fluorescence microscopy in paraffin sections, we performed a comparative assessment of the distribution of thiol-rich compounds in multiple epithelia of different species of intertidal P...
Source: Histochemistry and Cell Biology - June 7, 2020 Category: Biomedical Science Source Type: research

In focus in HCB
(Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - June 5, 2020 Category: Biomedical Science Source Type: research

CREB activity is required for mTORC1 signaling-induced primordial follicle activation in mice
AbstractIn mammals, progressive activation of primordial follicles is essential for maintenance of the reproductive lifespan. Several reports have demonstrated that mitogen-activated protein kinases 3 and 1 (MAPK3/1) –mammalian target of rapamycin complex 1 (mTORC1) signaling in pre-granulosa cells promotes primordial follicle activation by increasing KIT ligand (KITL) expression and then stimulating phosphatidylinositol 3 kinase signaling in oocytes. However, the mechanism of mTORC1 signaling in the promotion of KITL expression is unclear. Immunofluorescence staining results showed that phosphorylated cyclic AMP res...
Source: Histochemistry and Cell Biology - June 3, 2020 Category: Biomedical Science Source Type: research

Pcdh18a regulates endocytosis of E-cadherin during axial mesoderm development in zebrafish
AbstractThe notochord defines the axial structure of all vertebrates during development. Notogenesis is a result of major cell reorganization in the mesoderm, the convergence and the extension of the axial cells. However, it is currently not fully understood how these processes act together in a coordinated way during notochord formation. The prechordal plate is an actively migrating cell population in the central mesoderm anterior to the trailing notochordal plate cells. We show that prechordal plate cells express Protocadherin 18a (Pcdh18a), a member of the cadherin superfamily. We find that Pcdh18a-mediated recycling of...
Source: Histochemistry and Cell Biology - June 1, 2020 Category: Biomedical Science Source Type: research

Gene UCHL1 expresses specifically in mouse uterine decidual cells in response to estrogen
AbstractUCHL1 is expressed specifically in the brain and gonads of almost all studied model organisms includingDrosophila, zebrafish, amphibians, and mammals, suggesting a high degree of evolutionary conservation in its structure and function. Although UCHL1 has been involved in spermatogenesis in mice, its specific expression in mammal placenta remains elusive. Our previous work has revealed that UCHL1 is highly expressed in oocytes, and has been involved in mouse ovarian follicular development. Here, we further examined UCHL1 expression change in endometria during early natural pregnancy, with different stages of the est...
Source: Histochemistry and Cell Biology - May 25, 2020 Category: Biomedical Science Source Type: research

Developmental patterns in human blood –brain barrier and blood–cerebrospinal fluid barrier ABC drug transporter expression
In conclusion, human BBB and BCSFB ABC membrane transporters show brain location and transporter-specific maturation. (Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - May 24, 2020 Category: Biomedical Science Source Type: research

Effect of light on the calretinin and calbindin expression in skin club cells of adult zebrafish
In this study we demonstrated, for the first time, the presence of calretinin and calbindin D28K in skin club cells ofDanio rerio exposed to different wavelengths by immunohistochemistry analysis. Exposure to white-blue light and blue light causes the expression and colocalization of calbindin-D28K and calretinin. These proteins were moderately expressed and no colocalization was observed in the club cells of the control fish. In zebrafish exposed to continuous darkness for 10  days, in the club cells the two antibodies did not detect any proteins specifically. These results demonstrate that calbindin and calretinin c...
Source: Histochemistry and Cell Biology - May 20, 2020 Category: Biomedical Science Source Type: research

Sirtuin1 expression and survival in endometrial and clear-cell uterine cancer
In this study, we evaluated and correlated the SIRT1 expression with patients ’ tumor characteristics in endometrioid and clear-cell cancer of the uterus. 65 paraffin-embedded samples of patients with endometrial and clear-cell cancer of the uterus were immunohistochemically stained and SIRT1 expression was evaluated by immunoreactive score. The results were correlated to c linical and pathological tumor characteristics as well as to the expression of ARID1A and β-Catenin. The staining was significantly more intensive in uterine endometrioid carcinoma compared to uterine clear-cell carcinoma (p = 0...
Source: Histochemistry and Cell Biology - May 9, 2020 Category: Biomedical Science Source Type: research

In focus in HCB
(Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - May 8, 2020 Category: Biomedical Science Source Type: research

Six-transmembrane epithelial antigen of the prostate 1 expression promotes ovarian cancer metastasis by aiding progression of epithelial-to-mesenchymal transition
AbstractOvarian cancer is a severe malignant tumour of the female genital organs. Six-transmembrane epithelial antigen of the prostate 1 (STEAP1) expression is correlated with the occurrence and progression of multiple cancers. Here, we assessed STEAP1 expression in ovarian cancer and explored the relationship between STEAP1 and ovarian cancer progression. We used immunohistochemistry and public databases to test STEAP1 expression in normal human ovarian tissues, benign ovarian tumours, and ovarian cancer. The expression of STEAP1 and epithelial-to-mesenchymal transition (EMT)-related genes was analysed using immunocytoche...
Source: Histochemistry and Cell Biology - May 7, 2020 Category: Biomedical Science Source Type: research

Protein expression pattern of the molecular chaperone Mdg1/ERdj4 during embryonic development
AbstractThe vertebrate-specific co-chaperone Mdg1/ERdj4, which is localized in the endoplasmic reticulum, controls the folding and degradation of proteins. We characterized its protein pattern during chick embryonic development. During early development, Mdg1/ERdj4 protein is present in mesenchymal and epithelial cells. In mesenchymal cells, it has a salt and pepper pattern. In contrast, during epithelial tissue differentiation, Mdg1/ERdj4 marks the basal and/or apical compartment of epithelial linings. The distinct protein pattern in epithelial tissue might point to its role in organizing and maintaining the epithelial st...
Source: Histochemistry and Cell Biology - May 7, 2020 Category: Biomedical Science Source Type: research

Molecular analysis of H & amp;E- and Papanicolau-stained samples —systematic review
AbstractMolecular pathology allows the identification of causative agents in infectious diseases and detection of biomarkers important for prediction of disease susceptibility, diagnosis and personalized therapy. Accordingly, nucleic acid-based methods have gained a special role in clinical laboratories particularly to evaluate solid and hematological tumors. Extraction of nucleic acids is commonly performed in microdissected formalin-fixed paraffin-embedded (FFPE) or cytological samples that had been previously evaluated through the use of hematoxylin and eosin (H&E) or Papanicolau (Pap) stains, respectively. Although...
Source: Histochemistry and Cell Biology - May 5, 2020 Category: Biomedical Science Source Type: research

Influence of protein (human galectin-3) design on aspects of lectin activity
AbstractThe concept of biomedical significance of the functional pairing between tissue lectins and their glycoconjugate counterreceptors has reached the mainstream of research on the flow of biological information. A major challenge now is to identify the principles of structure –activity relationships that underlie specificity of recognition and the ensuing post-binding processes. Toward this end, we focus on a distinct feature on the side of the lectin, i.e. its architecture to present the carbohydrate recognition domain (CRD). Working with a multifunctional human lecti n, i.e. galectin-3, as model, its CRD is use...
Source: Histochemistry and Cell Biology - April 25, 2020 Category: Biomedical Science Source Type: research

Functional analysis of miR-767-5p during the progression of hepatocellular carcinoma and the clinical relevance of its dysregulation
AbstractAberrant microRNA (miRNA) expression is a central hallmark of hepatocellular carcinoma (HCC) and identification of the mechanisms underlying the miRNA actions should provide invaluable resource for revealing the molecular basis of different malignant behaviors in HCC. Previous high-throughput analysis has identified miR-767-5p as a unique miRNA signature of HCC, but the biological relevance and corresponding molecular basis of miR-767-5p in HCC is still in its infancy. The current study was, therefore, designed to elucidate whether changes in miR-767-5p expression levels affect HCC pathogenesis, and to further iden...
Source: Histochemistry and Cell Biology - April 24, 2020 Category: Biomedical Science Source Type: research

ASPP2 suppression promotes malignancy via LSR and YAP in human endometrial cancer
AbstractApoptosis-stimulating p53 protein 2 (ASPP2) is an apoptosis inducer that acts via binding with p53 and epithelial polarity molecule PAR3. Lipolysis-stimulated lipoprotein receptor (LSR) is an important molecule at tricellular contacts, and loss of LSR promotes cell migration and invasion via Yes-associated protein (YAP) in human endometrial cancer cells. In the present study, to find how ASPP2 suppression promotes malignancy in human endometrial cancer, we investigated its mechanisms including the relationship with LSR. In endometriosis and endometrial cancers (G1 and G2), ASPP2 was observed as well as PAR3 and LSR...
Source: Histochemistry and Cell Biology - April 7, 2020 Category: Biomedical Science Source Type: research

In focus in HCB
(Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - April 3, 2020 Category: Biomedical Science Source Type: research

Melanoma xenotransplant on the chicken chorioallantoic membrane: a complex biological model for the study of cancer cell behaviour
AbstractThe globally increasing incidence of cancer, including melanoma, requires novel therapeutic strategies. Development of successful novel drugs is based on clear identification of the target mechanisms responsible for the disease progression. The specific cancer microenvironment represents a critically important aspect of cancer biology, which cannot be properly studied in simplistic cell culture conditions. Among other traditional options, the study of melanoma cell growth on the chicken chorioallantoic membrane offers several significant advantages. This model offers increased complexity compared to usual in silico...
Source: Histochemistry and Cell Biology - March 30, 2020 Category: Biomedical Science Source Type: research

Nano-curcumin versus curcumin in amelioration of deltamethrin-induced hippocampal damage
In conclusion, oxidative stress is the common mechanism responsible for DLM-induced hippocampal neurotoxicity. It exerts apoptosis of hippocampal neurons through increasing Nrf2, HS-GCS, LS-GCS and decreasing AChE. In addition, it activates astr ocytes through increasing expression of GFAP. The protective role of CMN and CMMN is related to their potent antioxidant effect. Much improvement has been detected with NCMN as compared to CMN. (Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - March 29, 2020 Category: Biomedical Science Source Type: research

Mast cells and collagen fibrillogenesis
This article presents 20 combinations of histochemical stainings for the determination of mast cell co-localization with the fibrous component of the connective tissue in the fibrillogenesis course. Best results were obtained using metachromatic detection of mast cells in combination with silver or picro-fuchsin impregnation, staining with brilliant green using van Gieson staining, and a combination of aniline blue staining with neutral red. Proposed variants of histochemical protocols open up new opportunities to analyze the participation of mast cells in extracellular matrix remodeling of the tissue microenvironment in t...
Source: Histochemistry and Cell Biology - March 28, 2020 Category: Biomedical Science Source Type: research

Correction to: Accelerated proliferation of hepatocytes in rats with iron overload after partial hepatectomy
The figure shown below is the correct version. We apologize for the mistake. (Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - March 24, 2020 Category: Biomedical Science Source Type: research

Immunohistochemical detection of the pro-apoptotic Bax ∆2 protein in human tissues
AbstractThe pro-apoptotic Bax isoform Bax ∆2 was originally discovered in cancer patients with a microsatellite guanine deletion (G8 to G7). This deletion leads to an early stop codon; however, when combined with the alternative splicing of exon 2, the reading frame is restored allowing production of a full-length protein (Bax∆2). Unlik e the parental Baxα, Bax∆2 triggers apoptosis through a non-mitochondrial pathway and the expression in human tissues was unknown. Here, we analyzed over 1000 tissue microarray samples from 13 different organs using immunohistochemistry. Bax∆2-positive cells were detected in a...
Source: Histochemistry and Cell Biology - March 21, 2020 Category: Biomedical Science Source Type: research