A rapid and robust method for the cryopreservation of human granulosa cells
AbstractHuman primary granulosa cells (GCs) derived from women undergoing oocyte retrieval can be cultured and used as a cellular model for the study of human ovarian function. In vitro, they change rapidly, initially resembling cells of the preovulatory follicle and then cells of the corpus luteum. They are derived from individual patients, whose different medical history, lifestyle and age lead to heterogeneity. Thus, cells can rarely be ideally matched for cellular experiments or, if available, only in small quantities. We reasoned that cryopreservation of human GCs may be helpful to improve this situation. Previous stu...
Source: Histochemistry and Cell Biology - July 27, 2021 Category: Biomedical Science Source Type: research

The expression of diacylglycerol kinase isoforms α and ζ correlates with the progression of experimental autoimmune encephalomyelitis in rats
In this study, we analyzed the four DGK isozymes along the induction, peak and recovery phases in an experimental autoimmune encephalomyelitis (EAE) rat model of MS. The expression of thes e DGK isozymes and the diacylglycerol (DAG) pathway in the EAE rat brainstems were analyzed by qRT-PCR, immunohistochemistry, immunofluorescence double staining, western blotting and ELISA. Our results showed that the mRNA content of the four DGK isozymes decreased significantly, and their immunorea ctivity in myelin sheathes (DGKα, β) and neurons (DGKγ, ζ) became weaker at the beginning of the induction phase. With...
Source: Histochemistry and Cell Biology - July 26, 2021 Category: Biomedical Science Source Type: research

Stereology and three-dimensional reconstructions to analyze the pulmonary vasculature
AbstractThe pulmonary vasculature consists of a large arterial and venous tree with a vast alveolar capillary network (ACN) in between. Both conducting blood vessels and the gas-exchanging capillaries are part of important human lung diseases, including bronchopulmonary dysplasia, pulmonary hypertension and chronic obstructive pulmonary disease. Morphological tools to investigate the different parts of the pulmonary vasculature quantitatively and in three dimensions are crucial for a better understanding of the contribution of the blood vessels to the pathophysiology and effects of lung diseases. In recent years, new stere...
Source: Histochemistry and Cell Biology - July 16, 2021 Category: Biomedical Science Source Type: research

EBF1 is expressed in pericytes and contributes to pericyte cell commitment
AbstractEarly B-cell factor-1 (EBF1) is a transcription factor with an important role in cell lineage specification and commitment during the early stage of cell maturation. Originally described during B-cell maturation, EBF1 was subsequently identified as a crucial molecule for proper cell fate commitment of mesenchymal stem cells into adipocytes, osteoblasts and muscle cells. In vessels, EBF1 expression and function have never been documented. Our data indicate that EBF1 is highly expressed in peri-endothelial cells in both tumor vessels and in physiological conditions. Immunohistochemistry, quantitative reverse transcri...
Source: Histochemistry and Cell Biology - July 16, 2021 Category: Biomedical Science Source Type: research

Porcine ovarian cortex-derived putative stem cells can differentiate into endothelial cells in vitro
In conclusion, our results provided evidence that poPSCs may constitute the MSC population in the ovary and confirmed that they might be a potential source of ECs for tissue engineering. (Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - July 16, 2021 Category: Biomedical Science Source Type: research

Delayed maturation of thymic epithelium in mice with specific deletion of β-catenin gene in FoxN1 positive cells
AbstractWnt signalling pathways have been reported to be involved in thymus development but their precise role in the development of both thymic epithelium (TE) and thymocytes is controversial. Herein, we examined embryonic, postnatal and adult thymi of mice with a specific deletion of β-catenin gene in FoxN1+ thymic epithelial cells (TECs). Together with a high postnatal mouse mortality, the analysis showed severe thymic hypocellularity, largely due an important reduction in numbers of developing thymocytes, and delayed, partially blocked maturation of mutant TECs. Affected TECs included largely cortical (c) TEC subs...
Source: Histochemistry and Cell Biology - July 12, 2021 Category: Biomedical Science Source Type: research

In focus in HCB
(Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - July 7, 2021 Category: Biomedical Science Source Type: research

Correction to: Different localization of lysosomal-associated membrane protein 1 (LAMP1) in mammalian cultured cell lines
(Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - July 7, 2021 Category: Biomedical Science Source Type: research

Retraction Note to: XBP1S protects cells from ER stress-induced apoptosis through Erk1/2 signaling pathway involving CHOP
A correction to this paper has been published: https://doi.org/10.1007/s00418-021-02000-0 (Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - July 1, 2021 Category: Biomedical Science Source Type: research

CD9-positive cells in the intermediate lobe migrate into the anterior lobe to supply endocrine cells
AbstractThe adenohypophysis is composed of the anterior and intermediate lobes (AL and IL), and secretes important hormones for growth, sexual development, metabolism, and reproduction. In the marginal cell layer (MCL) facing Rathke ’s cleft between the IL and AL, cluster of differentiation (CD) 9-, CD81-, S100β-, and SOX2-quadruple positive (CD9/CD81/S100β/SOX2-positive) cells in the adult IL are settled as tissue-resident stem/progenitor cells supplying hormone-producing cells to the AL. However, it is unclear how CD9/CD8 1/S100β/SOX2-positive cells in the IL-side MCL migrate into the AL across Rathk...
Source: Histochemistry and Cell Biology - June 29, 2021 Category: Biomedical Science Source Type: research

Reprogramming of DNA methylation is linked to successful human preimplantation development
In conclusion, our results suggest that DNA methylation reprogramming is conserved in humans, with human-specific dynamics and extent. Furthermore, abnormalities in the four-cell-specific DNA methylome in early human embryogenesis are associated with abnormal development, highlighting an essential role of epigenetic reprogramming for successful human embryogenesis. Further research should identify the underlying genomic regions and cause of abnormal DNA methylation reprogramming in early human embryos. (Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - June 27, 2021 Category: Biomedical Science Source Type: research

Classification of human enteric neurons
AbstractMajor advances in our understanding of the functional heterogeneity of enteric neurons are driven by the application of newly developed, innovative methods. In contrast to this progress, both animal and human enteric neurons are usually divided into only two morphological subpopulations, “Dogiel type II” neurons (with several long processes) and “Dogiel type I” neurons (with several short processes). This implies no more than the distinction of intrinsic primary afferent from all other enteric neurons. The well-known chemical and functional diversity of enteric neurons is no t reflected by t...
Source: Histochemistry and Cell Biology - June 25, 2021 Category: Biomedical Science Source Type: research

Interaction between cadherins, vimentin, and V-set and immunoglobulin domain containing 1 in gastric-type hepatocellular carcinoma
We examined VSIG1 immunohistochemical (IHC) expression in 105 consecutive samples provided by HCC patients, along with the IHC expression of three of the biomarkers known to be involved in the epithelial –mesenchymal transition (EMT): vimentin (VIM), and E- and N-cadherin (encoded by CDH1 and CDH2 genes). IHC subcellular localization of thyroid transcription factor 1 (TTF1), in which nuclear-to-cytoplasmic translocation is known to cause a lineage shift from lung to gastric-type adenocarcinoma, wa s also checked. The obtained data were validated using the miRNET program. In the examined HCC samples, VSIG1 expression ...
Source: Histochemistry and Cell Biology - June 25, 2021 Category: Biomedical Science Source Type: research

Flow-through isolation of human first trimester umbilical cord endothelial cells
AbstractHuman umbilical vein and artery endothelial cells (HUVEC; HUAEC), placental endothelial cells (fpAEC), and endothelial colony-forming cells (ECFC) from cord blood are a widely used model for researching placental vascular development, fetal and placental endothelial function, and the effect of adverse conditions in pregnancy thereon. However, placental vascular development and angiogenesis start in the first weeks of gestation, and adverse conditions in pregnancy may also affect endothelial function before term, suggesting that endothelial cells from early pregnancy may respond differently. Thus, we established a n...
Source: Histochemistry and Cell Biology - June 24, 2021 Category: Biomedical Science Source Type: research

Nuclear expression of VDR and AHR is mutually exclusive in glandular cells in endometriosis
AbstractThe vitamin D receptor (VDR) and aryl hydrocarbon receptor (AHR) are two nuclear receptors that exert their effects by binding with ligands and forming a molecular complex. These complexes translocate to the nucleus and activate the expression of a series of genes which have a response element to VDR or AHR. Both receptors have been identified in the pathogenesis of endometriosis, a common disease characterized by the formation of endometrium-like tissue in ectopic zones. Despite numerous therapies, there is no definitive cure for endometriosis at the pharmacological level. Our study aims to describe the location a...
Source: Histochemistry and Cell Biology - June 21, 2021 Category: Biomedical Science Source Type: research

Imitating evolution ’s tinkering by protein engineering reveals extension of human galectin-7 activity
This study provides a clear direction for research on defining the full range of Gal-7 functionality and offers the perspective of testing applications for engineered heterodimers. (Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - June 21, 2021 Category: Biomedical Science Source Type: research

Correction to: Neurons expressing the aryl hydrocarbon receptor in the locus coeruleus and island of Calleja major are novel targets of dioxin in the mouse brain
A correction to this paper has been published: https://doi.org/10.1007/s00418-021-01997-8 (Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - June 18, 2021 Category: Biomedical Science Source Type: research

Subcellular dynamics of estrogen-related receptors involved in transrepression through interactions with scaffold attachment factor B1
AbstractEstrogen-related receptor (ERR), a member of the nuclear receptor superfamily, consists of three subtypes ( α, β, γ) and has strong homology with estrogen receptor. No endogenous ligands have been identified for ERRs, but they play key roles in metabolic, hormonal, and developmental processes as transcription factors without ligand binding. Although subnuclear dynamics are essential for nuclear events including nuclear receptor-mediated transcriptional regulation, the dynamics of ERRs are poorly understood. Here, we report that ERRs show subcellular kinetic changes in response to diethylstilbestrol...
Source: Histochemistry and Cell Biology - June 15, 2021 Category: Biomedical Science Source Type: research

atoh8 expression pattern in early zebrafish embryonic development
AbstractAtonal homologue 8 (atoh8) is a basic helix-loop-helix transcription factor expressed in a variety of embryonic tissues. While several studies have implicatedatoh8 in various developmental pathways in other species, its role in zebrafish development remains uncertain. So far, no studies have dealt with an in-depth in situ analysis of the tissue distribution ofatoh8 in embryonic zebrafish. We set out to pinpoint the exact location ofatoh8 expression in a detailed spatio-temporal analysis in zebrafish during the first 24  h of development (hpf). To our surprise, we observed transcription from pre-segmentation st...
Source: Histochemistry and Cell Biology - June 13, 2021 Category: Biomedical Science Source Type: research

The Golgi apparatus is the main microtubule-organizing center in differentiating skeletal muscle cells
In this study, we investigated the positional relationship between the microtubule nucleating sites and the Golgi apparatus in C2C12 myotubes and in primary cultured mouse skeletal myotubes. We focused on gaps in the perinuclear Golgi apparatus where the nuclear envelope was not covered with the Golgi apparatus. In microtubule regrowth assay, microtubule regrowth after cold-nocodazole depolymerization of preexisting microtubules was not found at the gap of the perinuclear Golgi apparatus. Most of the microtubule regrowth was detected at the CDK5RAP2 (CDK5 regulatory subunit-associated protein 2)-rich spots on the perinucle...
Source: Histochemistry and Cell Biology - June 10, 2021 Category: Biomedical Science Source Type: research

In focus in HCB
(Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - June 7, 2021 Category: Biomedical Science Source Type: research

Identification of testicular Foxq1 as a critical modulator of lactate metabolism in mouse Sertoli cells
AbstractPostmeiotic germ cells require the lactate produced by the adjacent Sertoli cells (SCs) as their sole energy fuels. Lactate production in SCs is elaborately regulated by monitoring the transcription of the lactate dehydrogenase A (Ldha) gene. However, the transcription factors that are responsible for the control ofLdha transcription in SCs remain ill defined. Herein, the expression of forkhead box Q1 (FOXQ1), a central modulator of glucose metabolism in liver, was demonstrated in mouse testis throughout postnatal development, with maximum levels in adult specimens. At this age, FOXQ1 was immunolocalized in the nuc...
Source: Histochemistry and Cell Biology - June 5, 2021 Category: Biomedical Science Source Type: research

Effects of supraphysiological vitamin D3 (cholecalciferol) supplement on normal adult rat ovarian functions
In conclusion, treating VD-sufficient female rats with supraphysiological VD3 supplements was not associated with hypercalcaemia, and could contribute to ovarian functions by regulating the hypothalamic-pituitary-ovarian hormones and ovarian VD-related molecules. However, further studies are still needed to illustrate the clinical significance of VD3 in female reproduction. (Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - June 1, 2021 Category: Biomedical Science Source Type: research

The distribution of phosphatidylinositol 4,5-bisphosphate in the budding yeast plasma membrane
AbstractPhosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) is generated through phosphorylation of phosphatidylinositol 4-phosphate (PtdIns(4)P) by Mss4p, the only PtdIns phosphate 5-kinase in yeast cells. PtdIns(4,5)P2 is involved in various kinds of yeast functions. PtdIns(4)P is not only the immediate precursor of PtdIns(4,5)P2, but also an essential signaling molecule in the plasma membrane, Golgi, and endosomal system. To analyze the distribution of PtdIns(4,5)P2 and PtdIns(4)P in the yeast plasma membrane at a nanoscale level, we employed a freeze –fracture electron microscopy (EM) method that physically imm...
Source: Histochemistry and Cell Biology - May 29, 2021 Category: Biomedical Science Source Type: research

Senescence and associated blood –brain barrier alterations in vitro
AbstractProgressive deterioration of the central nervous system (CNS) is commonly associated with aging. An important component of the neurovasculature is the blood –brain barrier (BBB), majorly made up of endothelial cells joined together by intercellular junctions. The relationship between senescence and changes in the BBB has not yet been thoroughly explored. Moreover, the lack of in vitro models for the study of the mechanisms involved in those changes im pede further and more in-depth investigations in the field. For this reason, we herein present an in vitro model of the senescent BBB and an initial attempt to ...
Source: Histochemistry and Cell Biology - May 27, 2021 Category: Biomedical Science Source Type: research

C(3)1-TAg in C57BL/6 J background as a model to study mammary tumor development
AbstractDiagnosis and prognosis of breast cancer is based on disease staging identified through histopathological and molecular biology techniques. Animal models are used to gain mechanistic insights into the development of breast cancer. C(3)1-TAg is a genetically engineered mouse model that develops mammary cancer. However, carcinogenesis caused by this transgene was characterized in the Friend Virus B (FVB) background. As most genetic studies are done in mice with C57BL/6 J background, we aimed to define the histological alterations in C3(1)-TAg C57BL/6 J animals. Our results showed that C3(1)-TAg animals with C57BL/6 J...
Source: Histochemistry and Cell Biology - May 18, 2021 Category: Biomedical Science Source Type: research

Selective involution of thymic medulla by cyclosporine A with a decrease of mature thymic epithelia, XCR1 + dendritic cells, and epithelium-free areas containing Foxp3 + thymic regulatory T cells
AbstractImmunosuppressive drugs such as cyclosporine A (CSA) can disrupt thymic structure and functions, ultimately inducing syngeneic/autologous graft-versus-host disease together with involuted medullas. To elucidate the effects of CSA on the thymus more precisely, we analyzed the effects of CSA on the thymus and T cell system using rats. In addition to confirming the phenomena already reported, we newly found that the proportion of recent thymic emigrants also greatly decreased, suggesting impaired supply. Immunohistologically, the medullary thymic epithelial cells (mTECs) presented with a relative decrease in the subse...
Source: Histochemistry and Cell Biology - May 16, 2021 Category: Biomedical Science Source Type: research

In focus in HCB
(Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - May 11, 2021 Category: Biomedical Science Source Type: research

Effects of histone deacetylase inhibitors Tricostatin A and Quisinostat on tight junction proteins of human lung adenocarcinoma A549 cells and normal lung epithelial cells
AbstractHistone deacetylase (HDAC) inhibitors have a potential therapeutic role for non-small cell lung cancer (NSCLC). However, more preclinical studies of HDAC inhibitors in NSCLC and normal lung epithelial cells are required to evaluate their antitumor activities and mechanisms. The bicellular tight junction molecule claudin-2 (CLDN-2) is highly expressed in lung adenocarcinoma tissues and increase the proliferation of adenocarcinoma cells. Downregulation of the tricellular tight junction molecule angulin-1/LSR induces malignancy via EGF-dependent CLDN-2 and TGF- β-dependent cellular metabolism in human lung adenoc...
Source: Histochemistry and Cell Biology - May 11, 2021 Category: Biomedical Science Source Type: research

Correction to: Dimethyl sulfoxide acutely enhances regulated insulin secretion in the MIN6-K8 mouse insulinoma cell line
A correction to this paper has been published: https://doi.org/10.1007/s00418-021-01988-9 (Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - May 9, 2021 Category: Biomedical Science Source Type: research

Neurons expressing the aryl hydrocarbon receptor in the locus coeruleus and island of Calleja major are novel targets of dioxin in the mouse brain
AbstractThe aryl hydrocarbon receptor (AhR) acts as a receptor that responds to ligands, including dioxin. The AhR –ligand complex translocates from the cytoplasm into the nucleus to induce gene expression. Because dioxin exposure impairs cognitive and neurobehavioral functions, AhR-expressing neurons need to be identified for elucidation of the dioxin neurotoxicity mechanism. Immunohistochemistry was performe d to detect AhR-expressing neurons in the mouse brain and confirm the specificity of the anti-AhR antibody usingAhr−/− mice. Intracellular distribution of AhR and expression level of AhR-target gene...
Source: Histochemistry and Cell Biology - May 8, 2021 Category: Biomedical Science Source Type: research

Scleraxis expressing scleral cells respond to inflammatory stimulation
AbstractThe sclera is an ocular tissue rich of collagenous extracellular matrix, which is built up and maintained by relatively few, still poorly characterized fibroblast-like cells. The aims of this study are to add to the characterization of scleral fibroblasts and to examine the reaction of these fibroblasts to inflammatory stimulation in an ex vivo organotypic model. Scleras of scleraxis-GFP (SCX-GFP) mice were analyzed using immunohistochemistry and qRT-PCR for the expression of the tendon cell associated marker genesscleraxis (SCX),mohawk andtenomodulin. In organotypic tissue culture, explanted scleras of adult scler...
Source: Histochemistry and Cell Biology - May 8, 2021 Category: Biomedical Science Source Type: research

In focus in HCB
(Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - April 13, 2021 Category: Biomedical Science Source Type: research

Methods for restoration of ki67 antigenicity in aged paraffin tissue blocks
AbstractPathology archives are a treasure trove of paraffin embedded tissue spanning many years and covering a wide variety of tissues and diseases. The possibility of using old archival formalin fixed paraffin embedded (FFPE) tissues for diagnostic updates and research projects is a widespread need and it requires archives of stable, well-preserved samples. Immunohistochemistry performed on old archival paraffin blocks may give unreliable results, in particular for some antigens, such as Ki67. In consideration of this phenomenon, our aim is to comprehensively test and identify methods which may be used to obtain Ki67 immu...
Source: Histochemistry and Cell Biology - April 10, 2021 Category: Biomedical Science Source Type: research

Current advances in the function and biogenesis of peroxisomes and their roles in health and disease
(Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - April 5, 2021 Category: Biomedical Science Source Type: research

Binding of gephyrin to microtubules is regulated by its phosphorylation at Ser270
AbstractGephyrin is a multifunctional scaffolding protein anchoring glycine- and subtypes of GABA type A- receptors at inhibitory postsynaptic membrane specializations by binding to the microtubule (MT) and/or the actin cytoskeleton. However, the conditions under which gephyrin can bind to MTs and its regulation are currently unknown. Here, we demonstrate that during the purification of MTs from rat brain by sedimentation of polymerized tubulin using high-speed centrifugation a fraction of gephyrin was bound to MTs, whereas gephyrin phosphorylated at the CDK5-dependent site Ser270 was detached from MTs and remained in the ...
Source: Histochemistry and Cell Biology - April 1, 2021 Category: Biomedical Science Source Type: research

In focus in HCB
(Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - March 28, 2021 Category: Biomedical Science Source Type: research

Early germline differentiation in bivalves: TDRD7 as a candidate investigational unit for Ruditapes philippinarum germ granule assembly
AbstractThe germline is a key feature of sexual animals and the ways in which it separates from the soma differ widely across Metazoa. However, at least at some point during germline differentiation, some cytoplasmic supramolecular structures (collectively called germ plasm-related structures) are present and involved in its specification and/or differentiation. The factors involved in the assembly of these granular structures are various and non-ubiquitous among animals, even if some functional patterns and the presence of certain domains appear to be shared among some. For instance, the LOTUS domain is shared by Oskar, t...
Source: Histochemistry and Cell Biology - March 26, 2021 Category: Biomedical Science Source Type: research

Organic dust exposure induces stress response and mitochondrial dysfunction in monocytic cells
We examined whether co-exposure to ethyl pyruvate (EP) or mitoapocynin (MA) could rescue ODE exposure induced mitochondrial changes. Transmission electron micrographs showed significant differences in cellular and organelle morphology upon ODE exposure. ODE exposure with and without EP co-treatment increased the mtDNA leakage into the cytosol. Next, ODE exposure increased PINK1, Parkin, cytoplasmic cytochrome c levels, and reduced mitochondrial mass and cell viability, indicating mitophagy. MA treatment was partially protective by decreasing Parkin expression, mtDNA and cytochrome c release and increasing cell viability. (...
Source: Histochemistry and Cell Biology - March 23, 2021 Category: Biomedical Science Source Type: research

Dimethyl sulfoxide acutely enhances regulated insulin secretion in the MIN6-K8 mouse insulinoma cell line
AbstractDiabetes mellitus is a metabolic disorder projected to afflict 700 million people globally by 2045. Fundamental to the progression of diabetes is an insufficient supply of insulin to meet metabolic demand. The MIN6-K8 cell line is a mouse insulinoma model of pancreatic β-cells frequently used to study the mechanisms of insulin secretion. Here, we evaluated the effects of short-term exposure to dimethyl sulfoxide (DMSO), a polar aprotic solvent commonly used in drug screening, on physiological characteristics of MIN6-K8 cells. Short-term exposure of MIN6-K8 cells to DMSO enhanced glucose-induced and tolbutamide...
Source: Histochemistry and Cell Biology - March 20, 2021 Category: Biomedical Science Source Type: research

Ghrelin peptide improves glial conditioned medium effects on neuronal differentiation of human adipose mesenchymal stem cells
AbstractThe influences of ghrelin on neural differentiation of adipose-derived mesenchymal stem cells (ASCs) were investigated in this study. The expression of typical neuronal markers, such as protein gene product 9.5 (PGP9.5) and Microtubule Associated Protein 2 (MAP2), as well as glial Fibrillary Acid Protein (GFAP) as a glial marker was evaluated in ASCs in different conditions. In particular, 2  µM ghrelin was added to control ASCs and to ASCs undergoing neural differentiation. For this purpose, ASCs were cultured in Conditioned Media obtained from Olfactory Ensheathing cells (OEC-CM) or from Schwann cells ...
Source: Histochemistry and Cell Biology - March 16, 2021 Category: Biomedical Science Source Type: research

Relationship between serum concentration, functional parameters and cell bioenergetics in IPEC-J2 cell line
AbstractThe foetal bovine serum (FBS) concentration could influence functional parameters of IPEC-J2 cells. IPEC-J2 is a non-transformed continuous epithelial cell line that represents an established in vitro model to study porcine gut inflammation and alterations of intestinal integrity. This cell line also represents a good translational model thanks to the high similitudes between pig and human gastrointestinal tract. With the aim to assess if the FBS-dependent functional variations are linked to the bioenergetic aspects, the addition of 5% and 10% FBS in the IPEC-J2 culture medium were tested. Doubling time and TEER me...
Source: Histochemistry and Cell Biology - March 16, 2021 Category: Biomedical Science Source Type: research

The distribution of liver cancer stem cells correlates with the mechanical heterogeneity of liver cancer tissue
In this study, we undertook a biophysical and biochemical assessment of the changes in the mechanical properties of liver tissue during the progression of liver cancer and explored the distribution of liver cancer stem cells in liver cancer tissues. Our analysis confirmed previous observations that the stiffness of liver tissue gradually increased with the progress of fibrosis. In liver cancer tissues, we found obvious mechanical heterogeneity: the core of the tumor was soft, the invasive front tissue was the hardest, and the para-cancer tissue was in an intermediate state. Interestingly, the greatest number of liver cance...
Source: Histochemistry and Cell Biology - March 12, 2021 Category: Biomedical Science Source Type: research

Examination of FERMT1 expression in placental chorionic villi and its role in HTR8-SVneo cell invasion
In this study, we examined the spatiotemporal pattern of expression of FERMT1 in human chorionic villi throughout gestation and its role in HTR8-SVneo substrate adhesion and invasion. Placental villous tissue was obtained from patients undergoing elective terminations at weeks 8–14, as well as from term deliveries at weeks 37–40 and analyzed by immunofluorescence. Additionally, HTR8-SVneo trophoblast cells were transfected with FERMT1-specific siRNA or non-targeting siRNA (control) and used in cell-subst rate adhesion as well as invasion assays. FERMT1 was primarily localized to membrane-associated regions at t...
Source: Histochemistry and Cell Biology - March 8, 2021 Category: Biomedical Science Source Type: research

Size-dependent biodistribution of thiol-organosilica nanoparticles and F4/80 protein expression in the genital tract of female mice after intravaginal administration
This study showed that, tOS-NPs size and type of tissue are important in biodistribution and uptake of tOS-NPs in the genital tract. Small size (100  nm) of tOS-NPs was highly accumulated in the genital tract tissues especially endometrial epithelium compared with large tOS-NPs (1000 nm). Contradictory, the large size induced the expression of F4/80 protein and the number of vaginal macrophages compared with small size. However, both small and large sizes of tOS-NPs were found co-localized with F4/80+ macrophages, located in the vaginal, endometrial and ovarian tissues. The tOS-NPs intravaginally administrated we...
Source: Histochemistry and Cell Biology - March 3, 2021 Category: Biomedical Science Source Type: research

Effects of supraphysiological vitamin D 3 (cholecalciferol) supplement on normal adult rat ovarian functions
In conclusion, treating VD-sufficient female rats with supraphysiological VD3 supplements was not associated with hypercalcaemia, and could contribute to ovarian functions by regulating the hypothalamic-pituitary-ovarian hormones and ovarian VD-related molecules. However, further studies are still needed to illustrate the clinical significance of VD3 in female reproduction. (Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - February 27, 2021 Category: Biomedical Science Source Type: research

5-HT containing enteroendocrine cells characterised by morphologies, patterns of hormone co-expression, and relationships with nerve fibres in the mouse gastrointestinal tract
We examined the morphologies of 5-HT cells from stomach to rectum, patterns of hormone co-expression in the stomach and colon, and the relationship of 5-HT cells with nerve fibres. We also reviewed some of the relevant literature. The morphologies of 5-HT cells were distinct, depending on their location in the gut. A noticeable feature of some 5-HT cells in the antrum and colon was their long basal processes, which resembled processes of neurons, whereas 5-HT cells in the small intestinal mucosa lacked basal processes. In the stomach, numerous 5-HT cells, including cells with basal processes, were identified as enterochrom...
Source: Histochemistry and Cell Biology - February 19, 2021 Category: Biomedical Science Source Type: research

Lung epithelial endoplasmic reticulum and mitochondrial 3D ultrastructure: a new frontier in lung diseases
AbstractIt has long been appreciated that the endoplasmic reticulum (ER) and mitochondria, organelles important for regular cell function and survival, also play key roles in pathogenesis of various lung diseases, including asthma, fibrosis, and infections. Alterations in processes regulated within these organelles, including but not limited to protein folding in the ER and oxidative phosphorylation in the mitochondria, are important in disease pathogenesis. In recent years it has also become increasingly apparent that organelle structure dictates function. It is now clear that organelles must maintain precise organization...
Source: Histochemistry and Cell Biology - February 18, 2021 Category: Biomedical Science Source Type: research

Preface: “Imaging the Lung in 3D: Pictures with Impact”
(Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - February 17, 2021 Category: Biomedical Science Source Type: research

Correction to: The distribution and chemical coding of enteroendocrine cells in Trypanosoma cruzi -infected individuals with chagasic megacolon
The correct affiliations are given in this correction. Patr ícia Rocha Martins 1; Josiane Fakhry 2; Adriana Jacaúna de Oliveira 3; Thayse Batista Moreira 3;  Linda J. Fothergill 2, 5; Enio Chaves de Oliveira 4;  Débora d’Ávila Reis 1; John B. Furness 2,5.  (Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - February 15, 2021 Category: Biomedical Science Source Type: research