Methods for restoration of ki67 antigenicity in aged paraffin tissue blocks
AbstractPathology archives are a treasure trove of paraffin embedded tissue spanning many years and covering a wide variety of tissues and diseases. The possibility of using old archival formalin fixed paraffin embedded (FFPE) tissues for diagnostic updates and research projects is a widespread need and it requires archives of stable, well-preserved samples. Immunohistochemistry performed on old archival paraffin blocks may give unreliable results, in particular for some antigens, such as Ki67. In consideration of this phenomenon, our aim is to comprehensively test and identify methods which may be used to obtain Ki67 immu...
Source: Histochemistry and Cell Biology - April 10, 2021 Category: Biomedical Science Source Type: research

Current advances in the function and biogenesis of peroxisomes and their roles in health and disease
(Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - April 5, 2021 Category: Biomedical Science Source Type: research

Binding of gephyrin to microtubules is regulated by its phosphorylation at Ser270
AbstractGephyrin is a multifunctional scaffolding protein anchoring glycine- and subtypes of GABA type A- receptors at inhibitory postsynaptic membrane specializations by binding to the microtubule (MT) and/or the actin cytoskeleton. However, the conditions under which gephyrin can bind to MTs and its regulation are currently unknown. Here, we demonstrate that during the purification of MTs from rat brain by sedimentation of polymerized tubulin using high-speed centrifugation a fraction of gephyrin was bound to MTs, whereas gephyrin phosphorylated at the CDK5-dependent site Ser270 was detached from MTs and remained in the ...
Source: Histochemistry and Cell Biology - April 1, 2021 Category: Biomedical Science Source Type: research

In focus in HCB
(Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - March 28, 2021 Category: Biomedical Science Source Type: research

Early germline differentiation in bivalves: TDRD7 as a candidate investigational unit for Ruditapes philippinarum germ granule assembly
AbstractThe germline is a key feature of sexual animals and the ways in which it separates from the soma differ widely across Metazoa. However, at least at some point during germline differentiation, some cytoplasmic supramolecular structures (collectively called germ plasm-related structures) are present and involved in its specification and/or differentiation. The factors involved in the assembly of these granular structures are various and non-ubiquitous among animals, even if some functional patterns and the presence of certain domains appear to be shared among some. For instance, the LOTUS domain is shared by Oskar, t...
Source: Histochemistry and Cell Biology - March 26, 2021 Category: Biomedical Science Source Type: research

Organic dust exposure induces stress response and mitochondrial dysfunction in monocytic cells
We examined whether co-exposure to ethyl pyruvate (EP) or mitoapocynin (MA) could rescue ODE exposure induced mitochondrial changes. Transmission electron micrographs showed significant differences in cellular and organelle morphology upon ODE exposure. ODE exposure with and without EP co-treatment increased the mtDNA leakage into the cytosol. Next, ODE exposure increased PINK1, Parkin, cytoplasmic cytochrome c levels, and reduced mitochondrial mass and cell viability, indicating mitophagy. MA treatment was partially protective by decreasing Parkin expression, mtDNA and cytochrome c release and increasing cell viability. (...
Source: Histochemistry and Cell Biology - March 23, 2021 Category: Biomedical Science Source Type: research

Dimethyl sulfoxide acutely enhances regulated insulin secretion in the MIN6-K8 mouse insulinoma cell line
AbstractDiabetes mellitus is a metabolic disorder projected to afflict 700 million people globally by 2045. Fundamental to the progression of diabetes is an insufficient supply of insulin to meet metabolic demand. The MIN6-K8 cell line is a mouse insulinoma model of pancreatic β-cells frequently used to study the mechanisms of insulin secretion. Here, we evaluated the effects of short-term exposure to dimethyl sulfoxide (DMSO), a polar aprotic solvent commonly used in drug screening, on physiological characteristics of MIN6-K8 cells. Short-term exposure of MIN6-K8 cells to DMSO enhanced glucose-induced and tolbutamide...
Source: Histochemistry and Cell Biology - March 20, 2021 Category: Biomedical Science Source Type: research

Ghrelin peptide improves glial conditioned medium effects on neuronal differentiation of human adipose mesenchymal stem cells
AbstractThe influences of ghrelin on neural differentiation of adipose-derived mesenchymal stem cells (ASCs) were investigated in this study. The expression of typical neuronal markers, such as protein gene product 9.5 (PGP9.5) and Microtubule Associated Protein 2 (MAP2), as well as glial Fibrillary Acid Protein (GFAP) as a glial marker was evaluated in ASCs in different conditions. In particular, 2  µM ghrelin was added to control ASCs and to ASCs undergoing neural differentiation. For this purpose, ASCs were cultured in Conditioned Media obtained from Olfactory Ensheathing cells (OEC-CM) or from Schwann cells ...
Source: Histochemistry and Cell Biology - March 16, 2021 Category: Biomedical Science Source Type: research

Relationship between serum concentration, functional parameters and cell bioenergetics in IPEC-J2 cell line
AbstractThe foetal bovine serum (FBS) concentration could influence functional parameters of IPEC-J2 cells. IPEC-J2 is a non-transformed continuous epithelial cell line that represents an established in vitro model to study porcine gut inflammation and alterations of intestinal integrity. This cell line also represents a good translational model thanks to the high similitudes between pig and human gastrointestinal tract. With the aim to assess if the FBS-dependent functional variations are linked to the bioenergetic aspects, the addition of 5% and 10% FBS in the IPEC-J2 culture medium were tested. Doubling time and TEER me...
Source: Histochemistry and Cell Biology - March 16, 2021 Category: Biomedical Science Source Type: research

The distribution of liver cancer stem cells correlates with the mechanical heterogeneity of liver cancer tissue
In this study, we undertook a biophysical and biochemical assessment of the changes in the mechanical properties of liver tissue during the progression of liver cancer and explored the distribution of liver cancer stem cells in liver cancer tissues. Our analysis confirmed previous observations that the stiffness of liver tissue gradually increased with the progress of fibrosis. In liver cancer tissues, we found obvious mechanical heterogeneity: the core of the tumor was soft, the invasive front tissue was the hardest, and the para-cancer tissue was in an intermediate state. Interestingly, the greatest number of liver cance...
Source: Histochemistry and Cell Biology - March 12, 2021 Category: Biomedical Science Source Type: research

Examination of FERMT1 expression in placental chorionic villi and its role in HTR8-SVneo cell invasion
In this study, we examined the spatiotemporal pattern of expression of FERMT1 in human chorionic villi throughout gestation and its role in HTR8-SVneo substrate adhesion and invasion. Placental villous tissue was obtained from patients undergoing elective terminations at weeks 8–14, as well as from term deliveries at weeks 37–40 and analyzed by immunofluorescence. Additionally, HTR8-SVneo trophoblast cells were transfected with FERMT1-specific siRNA or non-targeting siRNA (control) and used in cell-subst rate adhesion as well as invasion assays. FERMT1 was primarily localized to membrane-associated regions at t...
Source: Histochemistry and Cell Biology - March 8, 2021 Category: Biomedical Science Source Type: research

Size-dependent biodistribution of thiol-organosilica nanoparticles and F4/80 protein expression in the genital tract of female mice after intravaginal administration
This study showed that, tOS-NPs size and type of tissue are important in biodistribution and uptake of tOS-NPs in the genital tract. Small size (100  nm) of tOS-NPs was highly accumulated in the genital tract tissues especially endometrial epithelium compared with large tOS-NPs (1000 nm). Contradictory, the large size induced the expression of F4/80 protein and the number of vaginal macrophages compared with small size. However, both small and large sizes of tOS-NPs were found co-localized with F4/80+ macrophages, located in the vaginal, endometrial and ovarian tissues. The tOS-NPs intravaginally administrated we...
Source: Histochemistry and Cell Biology - March 3, 2021 Category: Biomedical Science Source Type: research

Effects of supraphysiological vitamin D 3 (cholecalciferol) supplement on normal adult rat ovarian functions
In conclusion, treating VD-sufficient female rats with supraphysiological VD3 supplements was not associated with hypercalcaemia, and could contribute to ovarian functions by regulating the hypothalamic-pituitary-ovarian hormones and ovarian VD-related molecules. However, further studies are still needed to illustrate the clinical significance of VD3 in female reproduction. (Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - February 27, 2021 Category: Biomedical Science Source Type: research

5-HT containing enteroendocrine cells characterised by morphologies, patterns of hormone co-expression, and relationships with nerve fibres in the mouse gastrointestinal tract
We examined the morphologies of 5-HT cells from stomach to rectum, patterns of hormone co-expression in the stomach and colon, and the relationship of 5-HT cells with nerve fibres. We also reviewed some of the relevant literature. The morphologies of 5-HT cells were distinct, depending on their location in the gut. A noticeable feature of some 5-HT cells in the antrum and colon was their long basal processes, which resembled processes of neurons, whereas 5-HT cells in the small intestinal mucosa lacked basal processes. In the stomach, numerous 5-HT cells, including cells with basal processes, were identified as enterochrom...
Source: Histochemistry and Cell Biology - February 19, 2021 Category: Biomedical Science Source Type: research

Lung epithelial endoplasmic reticulum and mitochondrial 3D ultrastructure: a new frontier in lung diseases
AbstractIt has long been appreciated that the endoplasmic reticulum (ER) and mitochondria, organelles important for regular cell function and survival, also play key roles in pathogenesis of various lung diseases, including asthma, fibrosis, and infections. Alterations in processes regulated within these organelles, including but not limited to protein folding in the ER and oxidative phosphorylation in the mitochondria, are important in disease pathogenesis. In recent years it has also become increasingly apparent that organelle structure dictates function. It is now clear that organelles must maintain precise organization...
Source: Histochemistry and Cell Biology - February 18, 2021 Category: Biomedical Science Source Type: research

Preface: “Imaging the Lung in 3D: Pictures with Impact”
(Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - February 17, 2021 Category: Biomedical Science Source Type: research

Correction to: The distribution and chemical coding of enteroendocrine cells in Trypanosoma cruzi -infected individuals with chagasic megacolon
The correct affiliations are given in this correction. Patr ícia Rocha Martins 1; Josiane Fakhry 2; Adriana Jacaúna de Oliveira 3; Thayse Batista Moreira 3;  Linda J. Fothergill 2, 5; Enio Chaves de Oliveira 4;  Débora d’Ávila Reis 1; John B. Furness 2,5.  (Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - February 15, 2021 Category: Biomedical Science Source Type: research

Introduction: 3D imaging in lung biology
(Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - February 11, 2021 Category: Biomedical Science Source Type: research

GluN2A- and GluN2B-immunoreactive type I cells attached to vesicular glutamate transporter 2-immunoreactive afferent nerve terminals of the rat carotid body
AbstractWe previously reported the immunoreactivity for the vesicular glutamate transporter 2 (VGLUT2) in afferent nerve terminals attached to chemoreceptor type I cells of the carotid body (CB), suggesting that glutamate is released from afferent terminals to stimulate these cells. In the present study, we examined the immunoreactivity for the glutamate-binding subunits ofN-methyl-D-aspartate (NMDA) receptors, GluN2A and GluN2B in the rat CB, and the immunohistochemical relationships between these subunits and VGLUT2. Immunoreactivities for GluN2A and GluN2B were predominant in a subpopulation of tyrosine hydroxylase-immu...
Source: Histochemistry and Cell Biology - February 7, 2021 Category: Biomedical Science Source Type: research

Second harmonic generation imaging of collagen scaffolds within the alveolar ducts of healthy and emphysematous mouse lungs
AbstractThe alveolar ducts are connected to peripheral septal fibers which extend from the visceral pleura into interlobular septa, and are anchored to axial fibers in the small airways. Together these axial and septal fibers constitute a fiber continuum that provides tension and integrity throughout the lung. Building on the observations that alveolar ducts associated with sub-pleural alveoli are orientated perpendicular to the visceral pleura, and in parallel to each other, the goal of the present study was to investigate the nature of the collagen fiber organization within sub-pleural alveolar ducts in healthy control a...
Source: Histochemistry and Cell Biology - January 30, 2021 Category: Biomedical Science Source Type: research

Steroid hormones and human choriogonadotropin influence the distribution of alpha6-integrin and desmoplakin 1 in gland-like endometrial epithelial spheroids
AbstractIn human glandular endometrial epithelial cells, desmosomal and adherens junction proteins have been shown to extend from a subapically restricted lateral position to the entire lateral membrane during the implantation window of the menstrual cycle. Similarly, a menstrual cycle stage-dependent redistribution of the extracellular matrix adhesion protein α6-integrin has been reported. These changes are believed to be important for endometrial receptiveness and successful embryo implantation. To prove the hypothesis that steroid hormones and human choriogonadotropin can induce the redistribution of these adhesio...
Source: Histochemistry and Cell Biology - January 27, 2021 Category: Biomedical Science Source Type: research

Wavelet decomposition facilitates training on small datasets for medical image classification by deep learning
AbstractThe adoption of low-dose computed tomography (LDCT) as the standard of care for lung cancer screening results in decreased mortality rates in high-risk population while increasing false-positive rate. Convolutional neural networks provide an ideal opportunity to improve malignant nodule detection; however, due to the lack of large adjudicated medical datasets these networks suffer from poor generalizability and overfitting. Using computed tomography images of the thorax from the National Lung Screening Trial (NLST), we compared discrete wavelet transforms (DWTs) against convolutional layers found in a CNN in order ...
Source: Histochemistry and Cell Biology - January 27, 2021 Category: Biomedical Science Source Type: research

Primary cilia presence and implications in bladder cancer progression and invasiveness
AbstractUrothelial bladder cancer is the tenth most common cancer worldwide. It is divided into muscle and non-muscle invading bladder cancer. Primary cilia have been related to several cancer hallmarks such as proliferation, epithelial-to-mesenchymal transition (EMT) or tumoral progression mainly through signaling pathways as Hedgehog (Hh). In the present study, we used immunohistochemical and ultrastructural techniques in human tissues of healthy bladder, non-muscle-invasive bladder cancer (NMIBC) and muscle-invasive bladder cancer (MIBC) to study and clarify the activation of epithelial-to-mesenchymal transition and Hed...
Source: Histochemistry and Cell Biology - January 25, 2021 Category: Biomedical Science Source Type: research

Protease profile of normal and neoplastic mast cells in the human bone marrow with special emphasis on systemic mastocytosis
We presented data on intracellular protease co-localization in human BM MCs and discussed their implica tion in secretory pathways of MCs in the development of the disease. Systemic mastocytosis, depending on the course, is featured by the formation of definite profiles of specific proteases in various forms of atypical mast cells. Intragranular accumulation of tryptase, chymase and carboxypeptidases in the hypochromic phenotype of atypical mast cells is characterized. Characterization of MC proteases expression during mastocytosis can be used to refine the MC classification, help in a prognosis, and increase the effective...
Source: Histochemistry and Cell Biology - January 25, 2021 Category: Biomedical Science Source Type: research

Correction to: Development of a simple, repeatable, and cost-effective extracellular matrix for long-term xeno-free and feeder-free self-renewal of human pluripotent stem cells
After publication of our article, it has come to our attention that our Conflict of Interest statement should read. (Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - January 25, 2021 Category: Biomedical Science Source Type: research

The calcium-activated chloride channel-associated protein rCLCA2 is expressed throughout rat epidermis, facilitates apoptosis and is downmodulated by UVB
AbstractThe rodent chloride channel regulatory proteins mCLCA2 and its porcine and human homologues pCLCA2 and hCLCA2 are expressed in keratinocytes but their localization and significance in the epidermis have remained elusive. hCLCA2 regulates cancer cell migration, invasion and apoptosis, and its loss predicts poor prognosis in many tumors. Here, we studied the influences of epidermal maturation and UV-irradiation (UVR) on rCLCA2 (previous rCLCA5) expression in cultured rat epidermal keratinocytes (REK) and correlated the results withmCLCA2 expression in mouse skin in vivo. Furthermore, we explored the influence of rCLC...
Source: Histochemistry and Cell Biology - January 23, 2021 Category: Biomedical Science Source Type: research

In focus in HCB
(Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - January 20, 2021 Category: Biomedical Science Source Type: research

Lung organoids: advances in generation and 3D-visualization
AbstractThe lung is comprised of more than 40 distinct cell types that support a complex 3-dimensional (3D) architecture that is required for efficient lung function. Loss of this proper architecture can accommodate and promote lung disease, highlighting researchers ’ growing need to analyze lung structures in detail. Additionally, in vivo cellular and molecular response to chemical and physical signals, along with the recapitulation of gene-expression patterns, can be lost during the transition from complex 3D tissues to 2D cell culture systems. Therefore, t echnologies that allow for the investigation of lung funct...
Source: Histochemistry and Cell Biology - January 18, 2021 Category: Biomedical Science Source Type: research

Localisation and regulation of cholesterol transporters in the human hair follicle: mapping changes across the hair cycle
AbstractCholesterol has long been suspected of influencing hair biology, with dysregulated homeostasis implicated in several disorders of hair growth and cycling. Cholesterol transport proteins play a vital role in the control of cellular cholesterol levels and compartmentalisation. This research aimed to determine the cellular localisation, transport capability and regulatory control of cholesterol transport proteins across the hair cycle. Immunofluorescence microscopy in human hair follicle sections revealed differential expression of ATP-binding cassette (ABC) transporters across the hair cycle. Cholesterol transporter ...
Source: Histochemistry and Cell Biology - January 6, 2021 Category: Biomedical Science Source Type: research

Simple method of thawing cryo-stored samples preserves ultrastructural features in electron microscopy
AbstractPreservation of ultrastructural features in biological samples for electron microscopy (EM) is a challenging task that is routinely accomplished through chemical fixation or high-pressure freezing coupled to automated freeze substitution (AFS) using specialized devices. However, samples from clinical (e.g. “biobanking” of bulk biopsies) and preclinical (e.g. whole mouse tissues) specimens are often not specifically prepared for ultrastructural analyses but simply immersed in liquid nitrogen before long-term cryo-storage. We demonstrate that ultrastructural features of such samples are insufficient ly co...
Source: Histochemistry and Cell Biology - January 6, 2021 Category: Biomedical Science Source Type: research

The distribution and chemical coding of enteroendocrine cells in Trypanosoma cruzi -infected individuals with chagasic megacolon
In this study, we investigated the distribution and chemical coding of EEC in the dilated and non-dilated portion ofT. cruzi-induced megacolon and in non-infected individuals (control colon). Using immunohistochemistry, EECs were identified by applying antibodies to chromogranin A (CgA), glucagon-like peptide 1 (GLP-1), 5-hydroxytryptamine (5-HT), peptide YY (PYY) and somatostatin (SST). Greater numbers of EEC expressing GLP-1 and SST occurred in the dilated portion compared to the non-dilated portion of the same patients with Chagas disease and in control colon, but numbers of 5-HT and PYY EEC were not significantly diffe...
Source: Histochemistry and Cell Biology - January 6, 2021 Category: Biomedical Science Source Type: research

Expression and enhancement of FABP4 in septoclasts of the growth plate in FABP5-deficient mouse tibiae
AbstractIn our previous study, fatty acid-binding protein 5 (FABP5) was expressed in septoclasts with long processes which are considered to resorb uncalcified matrix of the growth plate (GP) cartilage, and no apparent abnormalities were detected in the histo-architecture of the GP of FABP5-deficient (FABP5−/−) mice. Those finding lead us to hypothesize that another FABP can compensate the deletion of FABP5 in septoclasts of its gene-mutant mice. Based on the hypothesis, the present study examined the expression levels of several other FABPs in septoclasts and their morphology in FABP5−/− mouse tibi...
Source: Histochemistry and Cell Biology - January 4, 2021 Category: Biomedical Science Source Type: research

Perivascular macrophages produce type I collagen around cerebral small vessels under prolonged hypertension in rats
In this study, we examined if perivascular macrophages (PVMs) are involved in collagen production around cerebral small vessels in hypertensive SHRSP/Izm rats. Immunoreactivity for type I collagen around cerebral small vessels in 12-week-old hypertensive rats tended to higher than those in 4-week-old hypertensive and 12-week-old control rats. In ultrastructural analyses using transmission electron microscopy, the substantial deposition of collagen fibers could be observed in the intercellular spaces around PVMs near the arterioles of rats with prolonged hypertension. In situ hybridization analyses revealed that cells posit...
Source: Histochemistry and Cell Biology - January 4, 2021 Category: Biomedical Science Source Type: research

Expression analysis of neuropeptide FF receptors on neuroendocrine-related neurons in the rat brain using highly sensitive in situ hybridization
This study aimed to determine the detailed histological distribution of mRNA that encodes the neuropeptide FF receptors (Npffr1 andNpffr2) in the rat brain using in situ hybridization. Neurons with strongNpffr1 expression were observed in the lateral septal nucleus and several hypothalamic areas related to neuroendocrine functions, including the paraventricular nucleus (PVN) and arcuate nucleus, whereasNpffr2-expressing neurons were observed mainly in brain regions involved in somatosensory pathways, such as several subnuclei of the thalamus.Npffr1 expression was observed in 70% of corticotropin-releasing hormone neurons, ...
Source: Histochemistry and Cell Biology - January 4, 2021 Category: Biomedical Science Source Type: research

Protein expression pattern of calcium-responsive transactivator in early postnatal and adult testes
In this study, we examined the distribution of CREST in the adult testes of both rats and human as well as the expression pattern of CREST in the testes of postnatal developing rats. In the adult testes of both human and rats, immunohistochemical analysis revealed that CREST was selectively distributed in the mature Sertoli cells but not in the spermatogenic cells. In the testes of postnatal developmental rats, CREST was expressed not only in Sertoli cells but also in the gonocytes and spermatogenic cells at the initial stage of spermatogenic cell differentiation. CREST immunoreactivity continued to increase in Sertoli cel...
Source: Histochemistry and Cell Biology - January 4, 2021 Category: Biomedical Science Source Type: research

A combinatorial method to visualize the neuronal network in the mouse spinal cord: combination of a modified Golgi-Cox method and synchrotron radiation micro-computed tomography
In this study, to obtain high-resolution 3D imaging of a detailed neuronal network in the mass of the spinal cord, the combination of synchrotron radiation micro-computed tomography (SR μCT) and the Golgi-cox staining were used. We optimized the Golgi-Cox method (GCM) and developed a modified GCM (M-GCM), which improved background staining, reduced the number of artefacts, and diminished the impact of incomplete vasculature compared to the current GCM. Moreover, we achieved high-r esolution 3D imaging of the detailed neuronal network in the spinal cord through the combination of SRμCT and M-GCM. Our results showed th...
Source: Histochemistry and Cell Biology - January 4, 2021 Category: Biomedical Science Source Type: research

Diverse functions of myosin VI in spermiogenesis
AbstractSpermiogenesis is the final stage of spermatogenesis, a differentiation process during which unpolarized spermatids undergo excessive remodeling that results in the formation of sperm. The actin cytoskeleton and associated actin-binding proteins play crucial roles during this process regulating organelle or vesicle delivery/segregation and forming unique testicular structures involved in spermatid remodeling. In addition, several myosin motor proteins including MYO6 generate force and movement during sperm differentiation. MYO6 is highly unusual as it moves towards the minus end of actin filaments in the opposite d...
Source: Histochemistry and Cell Biology - January 2, 2021 Category: Biomedical Science Source Type: research

A comparison of airway pressures for inflation fixation of developing mouse lungs for stereological analyses
The objective of this study was to examine the impact of aPaw of 10, 20 and 30  cmH2O on the estimation of lung volumes and stereologically assessed parameters that describe the lung structure in developing mouse lungs. The data presented demonstrate that lowPaw (10 cmH2O) leads to heterogeneity in the unfolding of alveolar structures within the lungs, and that highPaw (30 cmH2O) leads to an overestimation of the lung volume, and thus, affects the estimation of volume-dependent parameters, such as total alveoli number and gas-exchange surface area. Thus, these data support the use of aPaw of 20  cmH2O for inflati...
Source: Histochemistry and Cell Biology - December 29, 2020 Category: Biomedical Science Source Type: research

miR-96-5p, miR-134-5p, miR-181b-5p and miR-200b-3p heterogenous expression in sites of prostate cancer versus benign prostate hyperplasia —archival samples study
AbstractMicroRNAs are involved in various pathologies including cancer. The aim of the study was to assess the level of expression of miR-96-5p, -134-5p, -181b-5p, -200b-3p in FFPE samples of prostate cancer, adjacent cancer-free tissue, and benign prostatic hyperplasia. Samples of 23 FFPE prostate cancer and 22 benign prostatic hyperplasias were dissected and HE stained. Compartments of tumor tissue and adjacent healthy glandular tissue were isolated from each sample using Laser Capture Microdissection. Total RNA was isolated from dissected tissues. Expression of miR-96-5p, miR-134-5p, 181b-5p, and miR-200b-3p was determi...
Source: Histochemistry and Cell Biology - December 17, 2020 Category: Biomedical Science Source Type: research

 In focus in HCB
(Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - December 4, 2020 Category: Biomedical Science Source Type: research

Combination of µCT and light microscopy for generation-specific stereological analysis of pulmonary arterial branches: a proof-of-concept study
In conclusion, the correlative image analysis of µCT and LM-based datasets is an innovative method to assess the pulmonary vasculature quantitatively. (Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - December 2, 2020 Category: Biomedical Science Source Type: research

Characterization of epithelial cells, connective tissue cells and immune cells in human upper airway mucosa by immunofluorescence multichannel image cytometry: a pilot study
This study provided quantitative data for the distribution of crucial cell types in CRS. Future studies may focus on the distribution and coexpression patterns of different immune cells in CRS or even cancer tissue. (Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - November 29, 2020 Category: Biomedical Science Source Type: research

Cluster of differentiation (CD) 9-positive mouse pituitary cells are adult stem/progenitor cells
AbstractSOX2-positive cells are stem/progenitor cells that supply hormone-producing cells; they are found in the anterior lobe of the rodent pituitary gland. However, they are likely composed of several subpopulations. In rats, a SOX2-positive cell populations can be distinguished by the presence of S100 β. We identified the novel markers cluster of differentiation (CD) CD9 and CD81, members of the tetraspanin superfamily, for the identification of S100β/SOX2-positive cells. Recently, CD9/CD81 double-knockout mice were generated. Although they grew normally until 3 weeks after birth, they exhibit ed atrophy ...
Source: Histochemistry and Cell Biology - November 22, 2020 Category: Biomedical Science Source Type: research

Human amniotic fluid stem cells are able to form embryoid body-like aggregates which performs specific functions: morphological evidences
AbstractHuman second trimester Amniotic Fluid Stem Cells (hAFSCs) harbour the potential to differentiate into cells of each of the three germ layers and to form Embryoid Body (EB)-like aggregates, without inducing teratoma formation and with no ethical concerns. However, in spite of the number of reports on hAFSCs-EBs and their characterization, a thorough evaluation in light and electron microscopy of morphological and morphometric features of hAFSCs-EBs development in vitro has not been reported yet. Apart from a superficial layer of epithelial-like flat cells, displaying rare microvilli on the free surface, hAFSCs-EBs e...
Source: Histochemistry and Cell Biology - November 21, 2020 Category: Biomedical Science Source Type: research

Mechanical ventilation-induced alterations of intracellular surfactant pool and blood –gas barrier in healthy and pre-injured lungs
In conclusion, mechanical ventilation-induced alterations in LB ultrastructure differ between healthy and pre-injured lungs. PEEP = 1 cmH2O but not PEEP  = 5 cmH2O ventilation aggravated septal interstitial abnormalities after bleomycin challenge. (Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - November 13, 2020 Category: Biomedical Science Source Type: research

Immunocytochemical assessment of cell differentiation of podoplanin-positive osteoblasts into osteocytes in murine bone
In this study, we examined the immunolocalization of podoplanin/E11, CD44, actin filaments, and phosphorylated ezrin in the osteoblasts on the verge of differentiating into osteocytes in murine femora and tibiae. When observing under stimulated emission depletion microscopy, unlike podoplanin-negative osteoblasts, podoplanin-positive osteoblasts showed a rearranged assembly of actin filaments along the cell membranes which resembled that of embedded osteocytes. In the metaphysis, i.e., the bone remodeling site, CD44-bearing osteoclasts were either proximal to or in contact with podoplanin-positive osteoblasts, but the podo...
Source: Histochemistry and Cell Biology - November 11, 2020 Category: Biomedical Science Source Type: research

Syndecan-3 contributes to the regulation of the microenvironment at the node of Ranvier following end-to ‑side neurorrhaphy: sodium image analysis
This study suggested syndecans may involve in stabilizing Nav1.6 and further modulate the distribution of sodium at nodal regions after remyelination. The efficiency of sodium re-clustering was improved by the assistance of anionic syndecan, resulting in a better funct ional repair of PNI. (Source: Histochemistry and Cell Biology)
Source: Histochemistry and Cell Biology - November 10, 2020 Category: Biomedical Science Source Type: research

Thioredoxin 1 is upregulated in the bone and bone marrow following experimental myocardial infarction: evidence for a remote organ response
AbstractIschemia and reperfusion events, such as myocardial infarction (MI), are reported to induce remote organ damage severely compromising patient outcomes. Tissue survival and functional restoration relies on the activation of endogenous redox regulatory systems such as the oxidoreductases of the thioredoxin (Trx) family. Trxs and peroxiredoxins (Prxs) are essential for the redox regulation of protein thiol groups and for the reduction of hydrogen peroxide, respectively. Here, we determined whether experimental MI induces changes in Trxs and Prxs in the heart as well as in secondary organs. Levels and localization of T...
Source: Histochemistry and Cell Biology - November 8, 2020 Category: Biomedical Science Source Type: research

A novel DENND1B-localized structure found at the basal side of adherent cells
In this study, we investigated the intracellular localization of DENN1B in various cell types and found novel DENND1B-localized gathered line structures in BS-C-1 cells and in some other cell types. The localization of DENND1B to gathered line structures was dependent on a specific region located in the C-terminus of DENND1B protein. DENND1B-localized gathered lines were partially associated with microtubules but not with F-actin; instead, F-actin bundles surrounded the assembly of gathered lines. We also show that the gathered line structures appeared at the bottom of spreading lamellipodia and disappeared at the retracti...
Source: Histochemistry and Cell Biology - November 2, 2020 Category: Biomedical Science Source Type: research

Potential functions of embryonic cardiac macrophages in angiogenesis, lymphangiogenesis and extracellular matrix remodeling
AbstractThe role of cardiac tissue macrophages (cTMs) during pre- and postnatal developmental stages remains in many aspects unknown. We aimed to characterize cTM populations and their potential functions based on surface markers. Our in situ studies of immunostained cardiac tissue specimens of murine fetuses (from E11to E17) revealed that a significant number of embryonic cTMs (phenotyped by CD45, CD68, CD64, F4/80, CD11b, CD206, Lyve-1) resided mostly in the subepicardial space, not in the entire myocardial wall, as observed in adult individuals. cTMs accompanied newly developed blood and lymphatic vessels adhering to ve...
Source: Histochemistry and Cell Biology - November 1, 2020 Category: Biomedical Science Source Type: research