Development of a duplex real-time RT-PCR assay for the detection and identification of two subgroups of human metapneumovirus in a single tube
In this study, we developed a real-time RT-PCR assay that can detect and identify the two major subgroups of hMPV (A and B) in one tube. Primers and probes were designed based on the sequences of recent clinical isolates in Japan. The assay showed comparable analytical sensitivity to a previously reported real-time RT-PCR assay and specific reactions to hMPV subgroups. The assay also showed no cross-reactivity to clinical isolates of 19 species of other respiratory viruses. In a validation assay using post-diagnosed clinical specimens, 98% (167/170) positivity was confirmed for the duplex assay, and the three specimens not...
Source: Journal of Virological Methods - September 23, 2023 Category: Virology Authors: Satoko Sugimoto Miyuki Kawase Reiko Suwa Masatoshi Kakizaki Yohei Kume Mina Chishiki Takashi Ono Hisao Okabe Sakurako Norito Mitsuaki Hosoya Koichi Hashimoto Kazuya Shirato Source Type: research
Serum steroid metabolite profiling by LC-MS/MS in two phenotypic male patients with HSD17B3 deficiency: implications for hormonal diagnosis
We examined two 46,XY Japanese patients with undermasculinized genitalia including hypospadias (patient 1 and patient 2). Endocrine studies by IA showed well increased serum T value after hCG stimulation (2.91ng/mL) and a high T/A-dione ratio (4.04) in patient 1 at 2 weeks of age and sufficiently elevated basal serum T value (2.60ng/mL) in patient 2 at 1.5 months of age. Despite such partial androgen insensitivity syndrome-like findings, whole exome sequencing identified biallelic ″pathogenic″ or ″likely pathogenic″ variants in HSD17B3 (c.188C>T:p.(Ala63Val) and c.194C>T:p.(Ser65Leu) in patient 1, and c.139A&...
Source: Molecular Medicine - September 23, 2023 Category: Molecular Biology Authors: Yasuko Fujisawa Yohei Masunaga Wataru Tanikawa Shinichi Nakashima Daisuke Ueda Shinichiro Sano Maki Fukami Hirotomo Saitsu Takashi Yazawa Tsutomu Ogata Source Type: research
Examining the Impact of Assistive Technology on Psychological Health, Family Education, and Curriculum Research in Japan: Insights from Artificial Intelligence
This study aims to analyze the effect of psychological health based on artificial intelligence agent technology on the implementation effect of Japanese family education. By combining mobile agent technology and education thought, the system structure and working mechanism of the education support system of agents are studied to build personalized support for the family education system based on mobile agents. A total of 320 Japanese middle school students were randomly divided into an experimental group and a control group, with 160 cases in each group. The control group received traditional family health education, while...
Source: Journal of Autism and Developmental Disorders - September 23, 2023 Category: Psychiatry Authors: Yanqi Guo Source Type: research
A comprehensive overview of investigational elastase inhibitors for the treatment of acute respiratory distress syndrome
Expert Opin Investig Drugs. 2023 Sep 23. doi: 10.1080/13543784.2023.2263366. Online ahead of print.ABSTRACTINTRODUCTION: Excessive activity of neutrophil elastase (NE), the main enzyme present in azurophil granules in the neutrophil cytoplasm, may cause tissue injury and remodeling in various lung diseases, including acute lung injury (ALI)/acute respiratory distress syndrome (ARDS), in which it is crucial to the immune response and inflammatory process. Consequently, NE is a possible target for therapeutic intervention in ALI/ARDS.AREAS COVERED: The protective effects of several NE inhibitors in attenuating ALI/ARDS in se...
Source: Expert Opinion on Investigational Drugs - September 23, 2023 Category: Drugs & Pharmacology Authors: Maria Gabriella Matera Paola Rogliani Josuel Ora Luigino Calzetta Mario Cazzola Source Type: research
Characterization of Actinobacillus pleuropneumoniae isolated from pigs in Japan using whole genome sequencing
Comp Immunol Microbiol Infect Dis. 2023 Sep 9;102:102062. doi: 10.1016/j.cimid.2023.102062. Online ahead of print.ABSTRACTWe conducted whole-genome sequencing to investigate the serotypes, the presence of virulence and antimicrobial resistance genes, and the genetic relationships among isolates of Actinobacillus. pleuropneumoniae derived from diseased pigs. Serotype 2 (71.2%) was the most common, but the prevalence of serotypes 6 (13.6%) and 15 (6.8%) increased. Existing vaccines are considered ineffective on the isolates belonging to serotypes 6 and 15. The phylogenetic tree based on core genome single nucleotide polymorp...
Source: Comparative immunology, microbiology and infectious diseases. - September 23, 2023 Category: Allergy & Immunology Authors: Manao Ozawa Motoshi Kawano Hitoshi Abo Yukari Issiki Mio Kumakawa Michiko Kawanishi Akemi Kojima Shoko Iwamoto Source Type: research
Impact of surgical treatment for intramedullary spinal cord metastasis on neurological function and survival: A multicenter retrospective study by the Neurospinal Society of Japan
CONCLUSIONS: This study suggests that surgical treatment can maintain or improve neurological function in a limited number of patients with ISCM, although it had minimal impact on improving the survival rate after surgery.PMID:37740999 | DOI:10.1016/j.jocn.2023.09.013 (Source: Clinical Lung Cancer)
Source: Clinical Lung Cancer - September 23, 2023 Category: Cancer & Oncology Authors: Motoyuki Iwasaki Kentaro Naito Toshiki Endo Yasukazu Hijikata Masaki Mizuno Minoru Hoshimaru Kazutoshi Hida Toshihiro Takami Study group for intramedullary spinal cord tumors in the Neurospinal Society of Japan Source Type: research
Impact of surgical treatment for intramedullary spinal cord metastasis on neurological function and survival: A multicenter retrospective study by the Neurospinal Society of Japan
CONCLUSIONS: This study suggests that surgical treatment can maintain or improve neurological function in a limited number of patients with ISCM, although it had minimal impact on improving the survival rate after surgery.PMID:37740999 | DOI:10.1016/j.jocn.2023.09.013 (Source: Clinical Breast Cancer)
Source: Clinical Breast Cancer - September 23, 2023 Category: Cancer & Oncology Authors: Motoyuki Iwasaki Kentaro Naito Toshiki Endo Yasukazu Hijikata Masaki Mizuno Minoru Hoshimaru Kazutoshi Hida Toshihiro Takami Study group for intramedullary spinal cord tumors in the Neurospinal Society of Japan Source Type: research
Impact of surgical treatment for intramedullary spinal cord metastasis on neurological function and survival: A multicenter retrospective study by the Neurospinal Society of Japan
CONCLUSIONS: This study suggests that surgical treatment can maintain or improve neurological function in a limited number of patients with ISCM, although it had minimal impact on improving the survival rate after surgery.PMID:37740999 | DOI:10.1016/j.jocn.2023.09.013 (Source: Clinical Genitourinary Cancer)
Source: Clinical Genitourinary Cancer - September 23, 2023 Category: Cancer & Oncology Authors: Motoyuki Iwasaki Kentaro Naito Toshiki Endo Yasukazu Hijikata Masaki Mizuno Minoru Hoshimaru Kazutoshi Hida Toshihiro Takami Study group for intramedullary spinal cord tumors in the Neurospinal Society of Japan Source Type: research
Impact of surgical treatment for intramedullary spinal cord metastasis on neurological function and survival: A multicenter retrospective study by the Neurospinal Society of Japan
CONCLUSIONS: This study suggests that surgical treatment can maintain or improve neurological function in a limited number of patients with ISCM, although it had minimal impact on improving the survival rate after surgery.PMID:37740999 | DOI:10.1016/j.jocn.2023.09.013 (Source: Clinical Colorectal Cancer)
Source: Clinical Colorectal Cancer - September 23, 2023 Category: Cancer & Oncology Authors: Motoyuki Iwasaki Kentaro Naito Toshiki Endo Yasukazu Hijikata Masaki Mizuno Minoru Hoshimaru Kazutoshi Hida Toshihiro Takami Study group for intramedullary spinal cord tumors in the Neurospinal Society of Japan Source Type: research
Examining the Impact of Assistive Technology on Psychological Health, Family Education, and Curriculum Research in Japan: Insights from Artificial Intelligence
This study aims to analyze the effect of psychological health based on artificial intelligence agent technology on the implementation effect of Japanese family education. By combining mobile agent technology and education thought, the system structure and working mechanism of the education support system of agents are studied to build personalized support for the family education system based on mobile agents. A total of 320 Japanese middle school students were randomly divided into an experimental group and a control group, with 160 cases in each group. The control group received traditional family health education, while...
Source: Journal of Autism and Developmental Disorders - September 23, 2023 Category: Psychiatry Authors: Yanqi Guo Source Type: research
Implementation of a Patient Summary Web Application According to the International Patient Summary and Validation in Common Use Cases in Japan
ConclusionsWe implemented a patient summary application that summarizes and visualizes patient information accumulated by existing systems and is evaluated in common use cases in Japan. Efficient sharing of the minimum necessary patient information for physicians is expected to reduce information overload, workload, and burnout. (Source: Journal of Medical Systems)
Source: Journal of Medical Systems - September 23, 2023 Category: Information Technology Source Type: research
Regulatory T cells in gastric cancer: key controllers from pathogenesis to therapy
Gastric cancer (GC) is a highly aggressive malignant disease responsible for over 700,000 deaths worldwide annually [1]. In 2020 alone, there were approximately 1.1 million new cases of GC, making it the fifth most common malignancy and the fourth leading cause of cancer-related death globally [2]. Asian countries, including Japan, Mongolia, and the Republic of Korea, exhibit a significantly higher incidence and prevalence of GC compared to Western countries [3]. Early-stage GC is often asymptomatic, necessitating screening upper gastrointestinal (GI) endoscopy with biopsy as the primary method for reliable diagnosis. (Sou...
Source: Cancer Treatment Reviews - September 23, 2023 Category: Cancer & Oncology Authors: Ion Negura, Mariana Pavel-Tanasa, Mihai Danciu Tags: Tumour Review Source Type: research
‘Mind-boggling’ sea creature spotted off Japan has finally been identified
When Ryo Minemizu, a professional underwater photographer, posted
photos
of an elegant ladybug-size flittering creature off Okinawa, Japan, in 2018, biologists were
baffled
. It wasn’t a worm, a mollusk, or a crustacean. What was it? No one knew.
One of those intrigued was Igor Adameyko, a developmental neurobiologist at the Medical University of Vienna. (A fellow enthusiast of marine biology, he spotted the pictures on Instagram, where he has his own
account
dedicated to marine zooplankton.) Adameyko asked Minemizu whether he had taken a specimen, and, if so, whether he might share it...
Source: Science of Aging Knowledge Environment - September 22, 2023 Category: Geriatrics Source Type: research
Bacillus subtilis Bacteremia from Gastrointestinal Perforation after Natto Ingestion, Japan
We report a case of Bacillus subtilis variant natto bacteremia from a gastrointestinal perforation in a patient who ingested natto. Genotypic methods showed the bacteria in a blood sample and the ingested natto were the same strains. Older or immunocompromised patients could be at risk for bacteremia from ingesting natto.PMID:37735785 | PMC:PMC10521625 | DOI:10.3201/eid2910.230084 (Source: Emerging Infectious Diseases)
Source: Emerging Infectious Diseases - September 22, 2023 Category: Infectious Diseases Authors: Takehiro Hashimoto Takaaki Yahiro Sakirul Khan Kazunori Kimitsuki Kazufumi Hiramatsu Akira Nishizono Source Type: research
Emergence of Novel Type C Botulism Strain in Household Outbreak, Japan
Emerg Infect Dis. 2023 Oct;29(10):2175-2177. doi: 10.3201/eid2910.230433.ABSTRACTIn 2021, an outbreak of food poisoning caused by Clostridium botulinum type C occurred in Kumamoto, Japan. Analysis of the isolated strain revealed that it possessed the bont/C gene and was slightly different from the reference bont/C gene. The risk for human infection with this new toxin type may be low.PMID:37735800 | PMC:PMC10521594 | DOI:10.3201/eid2910.230433 (Source: Emerging Infectious Diseases)
Source: Emerging Infectious Diseases - September 22, 2023 Category: Infectious Diseases Authors: Rika Maeda Misato Mori Seiya Harada Ichiro Izu Takaaki Hirano Yukie Inoue Shunsuke Yahiro Hiromi Koyama Source Type: research
