Epidemiology and Determinants of Vitamin D Deficiency in Eastern Nepal: A Community-Based, Cross-Sectional Study
CONCLUSION: The prevalence of vitamin D deficiency was relatively high in eastern Nepal. This highlights the need to create public awareness regarding the importance of bare skin sun exposure, nutritional sources of vitamin D, and the need to implement food fortification policies by the government.PMID:36118616 | PMC:PMC9481393 | DOI:10.1155/2022/1063163 (Source: International Journal of Endocrinology)
Source: International Journal of Endocrinology - September 19, 2022 Category: Endocrinology Authors: O Sherchand J K Baranwal B Gelal Source Type: research

Effects of two host-associated probiotics Bacillus mojavensis B191 and Bacillus subtilis MRS11 on growth performance, intestinal morphology, expression of immune-related genes and disease resistance of Nile tilapia (Oreochromis niloticus) against Streptococcusiniae
This study was conducted to assess the effects of dietary administration of two Bacillus strains on growth performance, intestinal morphology, immunity, and disease resistance of Nile tilapia. A total of 375 fish were randomly divided into five groups in triplicate. Nile tilapia were fed a basal diet (control group) or a basal diet supplemented with Bacillus mojavensis B191 (BM) or Bacillus subtilis MRS11 (BS) spores at different concentrations of 1 × 106 (BM6 and BS6, respectively) and 1 × 108 (BM8 and BS8, respectively) CFU/g of feed for 60 days. Moreover, the survival rate of tilapia upon challenge with Streptococcus ...
Source: Developmental and Comparative Immunology - September 19, 2022 Category: Allergy & Immunology Authors: Miray Etyemez B üyükdeveci İbrahim Cengizler Jos é L Balcázar İbrahim Demirkale Source Type: research

An overview of the role of metallic and nonmetallic nanoparticles and their salts during sperm cryopreservation and < em > in vitro < /em > embryo manipulation
Nucleosides Nucleotides Nucleic Acids. 2022 Sep 18:1-18. doi: 10.1080/15257770.2022.2124269. Online ahead of print.ABSTRACTThe cryopreservation of spermatozoa and the in vitro embryo production are valuable tools used in a variety of species, including humans, livestock, fish, and aquatic invertebrates. Sperm cryopreservation has been used to maintain or increase the genetic diversity of threatened species. Reactive oxygen species (ROS) are molecules derived from oxygen, being formed as byproducts of cellular metabolism. During cryopreservation of sperm and other in vitro manipulations of oocytes and embryos, ROS productio...
Source: Nucleosides, Nucleotides and Nucleic Acids - September 19, 2022 Category: Biochemistry Authors: Leila Soltani Hori Ghaneialvar Amir Hossein Mahdavi Source Type: research

Epidemiology and Determinants of Vitamin D Deficiency in Eastern Nepal: A Community-Based, Cross-Sectional Study
CONCLUSION: The prevalence of vitamin D deficiency was relatively high in eastern Nepal. This highlights the need to create public awareness regarding the importance of bare skin sun exposure, nutritional sources of vitamin D, and the need to implement food fortification policies by the government.PMID:36118616 | PMC:PMC9481393 | DOI:10.1155/2022/1063163 (Source: International Journal of Endocrinology)
Source: International Journal of Endocrinology - September 19, 2022 Category: Endocrinology Authors: O Sherchand J K Baranwal B Gelal Source Type: research

An overview of the role of metallic and nonmetallic nanoparticles and their salts during sperm cryopreservation and < em > in vitro < /em > embryo manipulation
Nucleosides Nucleotides Nucleic Acids. 2022 Sep 18:1-18. doi: 10.1080/15257770.2022.2124269. Online ahead of print.ABSTRACTThe cryopreservation of spermatozoa and the in vitro embryo production are valuable tools used in a variety of species, including humans, livestock, fish, and aquatic invertebrates. Sperm cryopreservation has been used to maintain or increase the genetic diversity of threatened species. Reactive oxygen species (ROS) are molecules derived from oxygen, being formed as byproducts of cellular metabolism. During cryopreservation of sperm and other in vitro manipulations of oocytes and embryos, ROS productio...
Source: Nucleosides, Nucleotides and Nucleic Acids - September 19, 2022 Category: Biochemistry Authors: Leila Soltani Hori Ghaneialvar Amir Hossein Mahdavi Source Type: research

Malignant pleural mesothelioma with heterologous elements
Conclusions Although median survival was longer for osteosarcoma than for malignant mesothelioma, we could not differentiate mesothelioma from pleural osteosarcoma on the combined basis of clinicopathological and immunohistochemical data, and FISH analysis. However, diffuse growth was more frequent in mesothelioma than in osteosarcoma. (Source: Journal of Clinical Pathology)
Source: Journal of Clinical Pathology - September 19, 2022 Category: Pathology Authors: Kawai, T., Seki, R., Miyajima, K., Nakashima, H., Takeda, T., Murakami, T., Aoe, K., Okabe, K., Homma, K., Tsukamoto, Y., Sunada, K., Terasaki, Y., Iida, M., Orikasa, H., Hiroshima, K. Tags: Original research Source Type: research

Viruses, Vol. 14, Pages 2080: Antiviral Activity of Crude Polysaccharide Derived from Seaweed against IHNV and IPNV In Vitro
n Lu Qiya Zhang Both infectious hematopoietic necrosis virus (IHNV) and infectious pancreatic necrosis virus (IPNV) are the causative agents of acute and highly contagious diseases of juvenile salmonids, resulting in severe economic losses to these cold-water fish globally. There is an urgent need to explore antiviral agents against IHNV and IPNV due to the lack of commercially available vaccines and antiviral drugs. More importantly, the co-infection of IHNV and IPNV is prevalent in nature, which not only aggravates extensive damage to the salmonids but also poses challenges to its prevention and control. The antivi...
Source: Viruses - September 19, 2022 Category: Virology Authors: Guangming Ren Liming Xu Jingzhuang Zhao Yizhi Shao Yujie Lin Linfang Li Qi Liu Tongyan Lu Qiya Zhang Tags: Article Source Type: research

Molecules, Vol. 27, Pages 6122: In Vivo Detection of Tetrodotoxin in Takifugu obscurus Based on Solid-Phase Microextraction Coupled with Ultrahigh-Performance Liquid Chromatography & ndash;Tandem Mass Spectrometry
In this study, the GO-PAN@PNE SPME fibers were made via a dipping method, and their extraction effect was verified in the TTX aqueous and spiked fish. The established method has good reproducibility, and the limit of detection of TTX in pufferfish was 32 ng&amp;middot;g&amp;minus;1, and the limit of quantitation was 150 ng&amp;middot;g&amp;minus;1, which can meet the detection needs of pufferfish for safe consumption. This method was used to in vivo detect the Takifugu obscurus exposed to the TTX, to determine the content of TTX in the pufferfish muscle. The detection method established in this study can re...
Source: Molecules - September 19, 2022 Category: Chemistry Authors: Hengli Meng Shui Jiang Yin Zhang Yun Hu Yuan Liu Tags: Article Source Type: research

Molecules, Vol. 27, Pages 6123: Assessment of the Effects of Chitosan, Chitooligosaccharides and Their Derivatives on Lemna minor
Isvoran Chitosan, chitooligosaccharides and their derivatives&amp;rsquo; production and use in many fields may result in their release to the environment, possibly affecting aquatic organisms. Both an experimental and a computational approach were considered for evaluating the effects of these compounds on Lemna minor. Based on the determined EC50 values against L. minor, only D-glucosamine hydrochloride (EC50 = 11.55 mg/L) was considered as &amp;ldquo;slightly toxic&amp;rdquo; for aquatic environments, while all the other investigated compounds, having EC50 &amp;gt; 100 mg/L, were considered as &am...
Source: Molecules - September 19, 2022 Category: Chemistry Authors: Bianca-Vanesa Boros Daniela Dascalu Vasile Ostafe Adriana Isvoran Tags: Article Source Type: research

An atlas of fish genome evolution reveals delayed rediploidization following the teleost whole-genome duplication [RESEARCH]
Teleost fishes are ancient tetraploids descended from an ancestral whole-genome duplication that may have contributed to the impressive diversification of this clade. Whole-genome duplications can occur via self-doubling (autopolyploidy) or via hybridization between different species (allopolyploidy). The mode of tetraploidization conditions evolutionary processes by which duplicated genomes return to diploid meiotic pairing, and subsequent genetic divergence of duplicated genes (cytological and genetic rediploidization). How teleosts became tetraploid remains unresolved, leaving a fundamental gap in the interpretation of ...
Source: Genome Research - September 19, 2022 Category: Genetics & Stem Cells Authors: Parey, E., Louis, A., Montfort, J., Guiguen, Y., Roest Crollius, H., Berthelot, C. Tags: RESEARCH Source Type: research