Gene expression analyses of GH/IGF axis in triploid crucian carp with growth heterosis
Hybridization and polyploid breeding are the main approaches used to obtain new aquaculture varieties. Allotriploid crucian carp (3n) with rapid growth performance was generated by mating red crucian carp (RCC) with allotetraploids (4n). Fish growth is controlled by the growth hormone (GH)/insulin-like growth factor (IGF) axis. In the present study, we examined the expression characteristics of GH/IGF axis genes in hybrids F1, 4n, 3n, RCC and common carp (CC). The results showed that GHRa, GHRb, IGF1, IGF2, and IGF-1Ra were highly expressed in 3n compared with RCC and CC, whereas IGF3 was undetectable in the liver in RCC, ...
Source: Frontiers in Endocrinology - March 26, 2024 Category: Endocrinology Source Type: research

The origins of gas exchange and ion regulation in fish gills: evidence from structure and function
AbstractGill function in gas exchange and ion regulation has played key roles in the evolution of fishes. In this review, we summarize data from the fields of palaeontology, developmental biology and comparative physiology for when and how the gills first acquired these functions. Data from across disciplines strongly supports a stem vertebrate origin for gas exchange structures and function at the gills with the emergence of larger, more active fishes. However, the recent discovery of putative ionocytes in extant cephalochordates and hemichordates suggests that ion regulation at gills might have originated much earlier th...
Source: Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology - March 26, 2024 Category: Physiology Source Type: research

A design of a nature-like fishway to solve the fractured river connectivity caused by small hydropower based on hydrodynamics and fish behaviors
In this study, based on the hydrodynamic characteristics of the river and the biological characteristics of fish, we evaluated the internal flow field in the nature-like fishway of Congen II hydropower station located along the Chabao river and explored methods to improve the operation efficiency. Based on comprehensive considerations of the flow field, turbulent kinetic energy, and the migration pathways of fish, it is found that the implementation of a continuous oblique bottom slope represents a more cost-effective and operationally convenient solution. The influence of different permutation of bulkheads in the nature-l...
Source: Environmental Science and Pollution Research International - March 25, 2024 Category: Environmental Health Authors: Jianing Rao Qi Wei Lian Tang Yuanming Wang Ruifeng Liang Kefeng Li Source Type: research

Assessing tropism and genetic traits of carp oedema virus isolates to enhance detection strategies
CONCLUSION: Determining the localisation of CEV genetic material in organs and tissues is pivotal for shaping the World Organisation for Animal Health guidelines. The utility of molecular diagnostics has been demonstrated in the skin and kidney of carp, in addition to the gills, impelling their inclusion in diagnostic protocols. The clusters identified in the phylogenetic tree offer valuable insights for developing the current PCR primers. The prevalence of CEV infection in aquaculture, juxtaposed with its notably lower detection in wild fish, underscores the significance of mandatory molecular diagnostic testing for CEV i...
Source: Cell Research - March 25, 2024 Category: Cytology Authors: Natalia Adamkowska Jolanta Kie łpińska Sven Michael Bergmann Source Type: research

The intracellular visualization of exogenous DNA in fluorescence microscopy
Drug Deliv Transl Res. 2024 Mar 25. doi: 10.1007/s13346-024-01563-4. Online ahead of print.ABSTRACTIn the development of non-viral gene delivery vectors, it is essential to reliably localize and quantify transfected DNA inside the cell. To track DNA, fluorescence microscopy methods are commonly applied. These mostly rely on fluorescently labeled DNA, DNA binding proteins fused to a fluorescent protein, or fluorescence in situ hybridization (FISH). In addition, co-stainings are often used to determine the colocalization of the DNA in specific cellular compartments, such as the endolysosomes or the nucleus. We provide an ove...
Source: Cell Research - March 25, 2024 Category: Cytology Authors: Christina Greitens Jean-Christophe Leroux Michael Burger Source Type: research

Atherosis-associated lnc_000048 activates PKR to enhance STAT1-mediated polarization of THP-1 macrophages to M1 phenotype
In this study, we established THP-1-derived testing state macrophages (M0), M1 macrophages, and alternately activated macrophages (M2). Real-time fluorescence quantitative PCR was used to verify the expression of marker genes and the expression of lnc_000048 in macrophages. Flow cytometry was used to detect phenotypic proteins (CD11b, CD38, CD80). We generated cell lines with lentivirus-mediated upregulation or downregulation of lnc_000048. Flow cytometry, western blot, and real-time fluorescence quantitative PCR results showed that down-regulation of lnc_000048 reduced M1 macrophage polarization and the inflammation respo...
Source: Cell Research - March 25, 2024 Category: Cytology Authors: Yuanyuan Ding Yu Sun Hongyan Wang Hongqin Zhao Ruihua Yin Meng Zhang Xudong Pan Xiaoyan Zhu Source Type: research

Catalase and Uric Acid Prevent Morphological Damage to the Sperm Flagella of < em > Colossoma macropomum < /em > During 96 Hours at Low Storage Temperatures
Biopreserv Biobank. 2024 Mar 22. doi: 10.1089/bio.2022.0213. Online ahead of print.ABSTRACTOxidative stress is one of the main causes of loss of sperm function during chilled storage. The aim of the current study was to evaluate the effects of a fructose-based extender, which was supplemented with catalase or uric acid, on the motility, viability, morphological integrity, and lipid peroxidation (LPO) of Colossoma macropomum spermatozoa. Sperm was diluted in extenders containing catalase (0; 0.1; 0.8; and 1.5 kU/L) or uric acid (0; 0.25; 0.5; and 1.0 mmol/L) and then stored at 4.3 ± 0.6°C for 96 hours. The chilling storag...
Source: Biopreservation and Biobanking - March 25, 2024 Category: Biomedical Science Authors: Yugo M Pastrana Jaydione L Marcon Amanda P de Amaral Francisco Bruno P Santos Emerson S Lima Leonard D R Acho Rodrigo Ot ávio S de Souza Carolina C Grando Danilo P Streit Junior Leandro Godoy Source Type: research

< em > RET < /em > Fusion Testing in Patients With NSCLC: The RETING Study
CONCLUSIONS: In-depth knowledge of the advantages and disadvantages of the different RET testing methodologies could help clinical and molecular tumor boards implement and maintain sensible algorithms for the rapid and effective detection of RET fusions in patients with NSCLC. The likelihood of RET false-negative results with both FISH and RT-PCR reinforces the need for upfront NGS in patients with NSCLC.PMID:38525319 | PMC:PMC10957499 | DOI:10.1016/j.jtocrr.2024.100653 (Source: Clinical Lung Cancer)
Source: Clinical Lung Cancer - March 25, 2024 Category: Cancer & Oncology Authors: Esther Conde Susana Hernandez Jose Luis Rodriguez Carrillo Rebeca Martinez Marta Alonso Daniel Curto Beatriz Jimenez Alejandra Caminoa Amparo Benito Pilar Garrido Sergi Clave Edurne Arriola Isabel Esteban-Rodriguez Javier De Castro Irene Sansano Enriqueta Source Type: research

Atherosis-associated lnc_000048 activates PKR to enhance STAT1-mediated polarization of THP-1 macrophages to M1 phenotype
In this study, we established THP-1-derived testing state macrophages (M0), M1 macrophages, and alternately activated macrophages (M2). Real-time fluorescence quantitative PCR was used to verify the expression of marker genes and the expression of lnc_000048 in macrophages. Flow cytometry was used to detect phenotypic proteins (CD11b, CD38, CD80). We generated cell lines with lentivirus-mediated upregulation or downregulation of lnc_000048. Flow cytometry, western blot, and real-time fluorescence quantitative PCR results showed that down-regulation of lnc_000048 reduced M1 macrophage polarization and the inflammation respo...
Source: Atherosclerosis - March 25, 2024 Category: Cardiology Authors: Yuanyuan Ding Yu Sun Hongyan Wang Hongqin Zhao Ruihua Yin Meng Zhang Xudong Pan Xiaoyan Zhu Source Type: research

Parenteral nutrition at home/long-term parenteral nutrition
CONCLUSION: Education and communication remain key components of a successful HPN process. The information shared here may help to motivate efforts to improve HPN processes and to consider the often-differing perspectives of patients and their healthcare professionals.PMID:38527076 | DOI:10.1093/ajhp/zxae081 (Source: American Journal of Health-System Pharmacy : AJHP)
Source: American Journal of Health-System Pharmacy : AJHP - March 25, 2024 Category: Drugs & Pharmacology Authors: Vanessa J Kumpf Brenda Gray Jessica Monczka Sarah Zeraschi Stanislaw Klek Source Type: research

Disruption of thyroid hormone receptor thrab leads to female infertility in zebrafish
Endocrinology. 2024 Mar 25:bqae037. doi: 10.1210/endocr/bqae037. Online ahead of print.ABSTRACTThyroid hormones (THs), thyroxine (T4) and triiodothyronine (T3), are vital for development, growth, and metabolism. Thyroid dysfunction can also cause problems in fertility, suggesting involvement of THs in reproduction. In zebrafish, there exist two forms of TH receptor alpha gene (THRα: thraa and thrab). Disruption of these genes by CRISPR/Cas9 showed no reproductive irregularities in thraa mutant; however, inactivation of thrab gene resulted in female infertility. Although young female mutants (thrabm/m) showed normal ovaria...
Source: Endocrinology - March 25, 2024 Category: Endocrinology Authors: Nana Ai Cho Rong Han Hui Zhao Sheue-Yann Cheng Wei Ge Source Type: research

Spatiotemporal variability and drivers of water microchemistry in the upper Nu-Salween river: With implications for fish habitat conservation
Environ Res. 2024 Mar 25;252(Pt 1):118754. doi: 10.1016/j.envres.2024.118754. Online ahead of print.ABSTRACTMicrochemical maps, also known as "chemoscapes", hold immense potential for reconstructing fish habitat utilization and guiding conservation efforts. This approach relies on matching the microchemical composition of fish calcified structures (e.g., otoliths) with the surrounding water's microchemistry. However, applying this method faces a major challenge: a clear understanding of the spatiotemporal variability and drivers of water microchemistry, particularly in vast, free-flowing river ecosystems like the Nu-Salwee...
Source: Environmental Research - March 25, 2024 Category: Environmental Health Authors: Bangli Tang Chengzhi Ding Liuyong Ding Yongtao Zhao Mengxue Zhang Juan Tao Source Type: research

A design of a nature-like fishway to solve the fractured river connectivity caused by small hydropower based on hydrodynamics and fish behaviors
In this study, based on the hydrodynamic characteristics of the river and the biological characteristics of fish, we evaluated the internal flow field in the nature-like fishway of Congen II hydropower station located along the Chabao river and explored methods to improve the operation efficiency. Based on comprehensive considerations of the flow field, turbulent kinetic energy, and the migration pathways of fish, it is found that the implementation of a continuous oblique bottom slope represents a more cost-effective and operationally convenient solution. The influence of different permutation of bulkheads in the nature-l...
Source: Environmental Science and Pollution Research International - March 25, 2024 Category: Environmental Health Authors: Jianing Rao Qi Wei Lian Tang Yuanming Wang Ruifeng Liang Kefeng Li Source Type: research

Catalase and Uric Acid Prevent Morphological Damage to the Sperm Flagella of < em > Colossoma macropomum < /em > During 96 Hours at Low Storage Temperatures
Biopreserv Biobank. 2024 Mar 22. doi: 10.1089/bio.2022.0213. Online ahead of print.ABSTRACTOxidative stress is one of the main causes of loss of sperm function during chilled storage. The aim of the current study was to evaluate the effects of a fructose-based extender, which was supplemented with catalase or uric acid, on the motility, viability, morphological integrity, and lipid peroxidation (LPO) of Colossoma macropomum spermatozoa. Sperm was diluted in extenders containing catalase (0; 0.1; 0.8; and 1.5 kU/L) or uric acid (0; 0.25; 0.5; and 1.0 mmol/L) and then stored at 4.3 ± 0.6°C for 96 hours. The chilling storag...
Source: Biopreservation and Biobanking - March 25, 2024 Category: Biomedical Science Authors: Yugo M Pastrana Jaydione L Marcon Amanda P de Amaral Francisco Bruno P Santos Emerson S Lima Leonard D R Acho Rodrigo Ot ávio S de Souza Carolina C Grando Danilo P Streit Junior Leandro Godoy Source Type: research

< em > RET < /em > Fusion Testing in Patients With NSCLC: The RETING Study
CONCLUSIONS: In-depth knowledge of the advantages and disadvantages of the different RET testing methodologies could help clinical and molecular tumor boards implement and maintain sensible algorithms for the rapid and effective detection of RET fusions in patients with NSCLC. The likelihood of RET false-negative results with both FISH and RT-PCR reinforces the need for upfront NGS in patients with NSCLC.PMID:38525319 | PMC:PMC10957499 | DOI:10.1016/j.jtocrr.2024.100653 (Source: Clinical Lung Cancer)
Source: Clinical Lung Cancer - March 25, 2024 Category: Cancer & Oncology Authors: Esther Conde Susana Hernandez Jose Luis Rodriguez Carrillo Rebeca Martinez Marta Alonso Daniel Curto Beatriz Jimenez Alejandra Caminoa Amparo Benito Pilar Garrido Sergi Clave Edurne Arriola Isabel Esteban-Rodriguez Javier De Castro Irene Sansano Enriqueta Source Type: research