Assessment of the FRET-based Teen sensor to monitor ERK activation changes preceding morphological defects in a RASopathy zebrafish model and phenotypic rescue by MEK inhibitor

CONCLUSION: This work proves the usefulness of FRET imaging protocols on both live and fixed Teen ERK reporter fish to readily monitor and quantify pharmacologically- and genetically-induced ERK activity modulations in early embryos, representing a useful tool in pre-clinical applications targeting RAS-MAPK signaling.PMID:38594640 | DOI:10.1186/s10020-024-00807-w

Source: Molecular Medicine - April 9, 2024 Category: Molecular Biology Authors: Giulia Fasano Stefania Petrini Valeria Bonavolont à Graziamaria Paradisi Catia Pedalino Marco Tartaglia Antonella Lauri Source Type: research