Low Dose of Nickel and Benzo [a] Anthracene in Rat-Diet, Induce Apoptosis, Fibrosis, and Initiate Carcinogenesis in Liver via NF- Ƙβ Pathway
This study was designed to evaluate the carcinogenic risk and other risks associated with long-term consumption of environmentally relevant dose of nickel and benzo [a] anthracene in rats. Thirty-six (36) male rats weighing between 80 and 100 g were assigned into 6 groups of 6 animals each; normal, nickel-, and benzo [a] anthracene-exposed groups for 12 and 24 weeks, respectively. Micronucleus and comet analyses were done in the blood, liver, and bone marrow. Liver function, redox, and inflammatory markers (AST, ALT, GGT, SOD, GSH, MDA, protein carbonyl, protein thiol, total protein, IL-10, 1L-1β, TNF-α, TGF-β NF-Ƙβ, ...
Source: Biological Trace Element Research - April 24, 2024 Category: Biology Authors: Adewale Adetutu Peter Ifeoluwa Adegbola Abiodun Bukunmi Aborisade Source Type: research
Ultrastructural, molecular and haemato-immunological changes: multifaceted toxicological effects of microcystin-LR in rohu, Labeo rohita
Chemosphere. 2024 Apr 22:142097. doi: 10.1016/j.chemosphere.2024.142097. Online ahead of print.ABSTRACTNo water body is resilient to afflicts of algal bloom, if goes unmanaged. With the increasing trend of intensification, eutrophication and climate change, Labeo rohita (rohu) is highly anticipated to suffer from the deleterious effects of bloom and eventually its toxins. A comprehensive study was conducted to understand the toxicopathological effects of microcystin-LR (MC-LR) in rohu following intraperitoneal injection of 96 h-LD50 dose i.e., 713 μg kg-1. Substantial changes in micro- and ultrastructural level were evide...
Source: Chemosphere - April 24, 2024 Category: Chemistry Authors: Snatashree Mohanty Anirban Paul Sagar Banerjee K V Rajendran Gayatri Tripathi Pratap Chandra Das Pramoda Kumar Sahoo Source Type: research
Thyroid and sex hormone disrupting effects of DEHTP at different life stages of zebrafish (Danio rerio)
Chemosphere. 2024 Apr 22:142105. doi: 10.1016/j.chemosphere.2024.142105. Online ahead of print.ABSTRACTDi(2-ethylhexyl) terephthalate (DEHTP) is an alternative plasticizer widely used in numerous consumer products, replacing di(2-ethylhexyl) phthalate (DEHP). Hence, DEHTP has been frequently detected in the environment and humans. As a structural isomer and functional analog of DEHP, DEHTP is a suspected endocrine disruptor. Here, we evaluated DEHTP thyroid-disrupting effects in embryo-larval and adult male zebrafish. We also investigated sex hormone disruption in the adult zebrafish. After 5- and 7-day exposure to DEHTP, ...
Source: Chemosphere - April 24, 2024 Category: Chemistry Authors: Yunchul Ihn Yoojin Cho Yura Lee Hyesun Seok Jin-Su Oh Hyo-Bang Moon Kyungho Choi Source Type: research
Growth and carbon turnover of Piaractus mesopotamicus Holmberg, 1887 (Osteicthyes: Characidae): contribution of extruded feed and natural food
This study evaluates effects of feed allowance on the productive performance, carbon turnover and proportions of nutrient (carbon) contribution of feed and natural food for the growth of pacu. Juvenile fish were stocked in fiberglass tanks and fed to 100, 75, 50, 25, 0% apparent satiety (ApS), with a practical, extruded (C4 photosynthetic pathway) feed in a randomized design trial (n=3); plankton production for simulated semi-intensive farming system condition was induced by chemical fertilization. A control treatment was set up in tanks devoid of natural food. Data on muscle stable carbon isotope ratios were used to study...
Source: An Acad Bras Cienc - April 24, 2024 Category: Science Authors: Fredy Armando A Aguilar Rafael S C Barone Gerson B Mour ão Luiz Antonio Martinelli Jos é Eurico P Cyrino Source Type: research
Global distribution patterns and geographic range of Cymothoa Fabricius, 1793 (Isopoda: Cymothoidae) associated with host fish
An Acad Bras Cienc. 2024 Apr 22;96(1):e20230706. doi: 10.1590/0001-3765202420230706. eCollection 2024.ABSTRACTOver recent years, fish parasites of the genus Cymothoa Fabricius, 1793, have received increased attention due to both their ecological and their economic importance to aquaculture and fishery. As the studies about Cymothoa have increased this improve our understanding on the host specificity and distribution of these parasites. The aim of this paper was to review the current global geographic distribution, distribution patterns and parasite-host interactions patterns of Cymothoa spp. associated with fish from mari...
Source: An Acad Bras Cienc - April 24, 2024 Category: Science Authors: Marcos Tavares-Dias Marcos Sidney B Oliveira Source Type: research
Ultrastructural, molecular and haemato-immunological changes: multifaceted toxicological effects of microcystin-LR in rohu, Labeo rohita
Chemosphere. 2024 Apr 22:142097. doi: 10.1016/j.chemosphere.2024.142097. Online ahead of print.ABSTRACTNo water body is resilient to afflicts of algal bloom, if goes unmanaged. With the increasing trend of intensification, eutrophication and climate change, Labeo rohita (rohu) is highly anticipated to suffer from the deleterious effects of bloom and eventually its toxins. A comprehensive study was conducted to understand the toxicopathological effects of microcystin-LR (MC-LR) in rohu following intraperitoneal injection of 96 h-LD50 dose i.e., 713 μg kg-1. Substantial changes in micro- and ultrastructural level were evide...
Source: Chemosphere - April 24, 2024 Category: Chemistry Authors: Snatashree Mohanty Anirban Paul Sagar Banerjee K V Rajendran Gayatri Tripathi Pratap Chandra Das Pramoda Kumar Sahoo Source Type: research
Thyroid and sex hormone disrupting effects of DEHTP at different life stages of zebrafish (Danio rerio)
Chemosphere. 2024 Apr 22:142105. doi: 10.1016/j.chemosphere.2024.142105. Online ahead of print.ABSTRACTDi(2-ethylhexyl) terephthalate (DEHTP) is an alternative plasticizer widely used in numerous consumer products, replacing di(2-ethylhexyl) phthalate (DEHP). Hence, DEHTP has been frequently detected in the environment and humans. As a structural isomer and functional analog of DEHP, DEHTP is a suspected endocrine disruptor. Here, we evaluated DEHTP thyroid-disrupting effects in embryo-larval and adult male zebrafish. We also investigated sex hormone disruption in the adult zebrafish. After 5- and 7-day exposure to DEHTP, ...
Source: Chemosphere - April 24, 2024 Category: Chemistry Authors: Yunchul Ihn Yoojin Cho Yura Lee Hyesun Seok Jin-Su Oh Hyo-Bang Moon Kyungho Choi Source Type: research
Novel aerobic anoxygenic phototrophic bacterium Jannaschia pagri sp. nov., isolated from seawater around a fish farm
Antonie Van Leeuwenhoek. 2024 Apr 24;117(1):70. doi: 10.1007/s10482-024-01971-z.ABSTRACTThe genus Jannaschia is one of the representatives of aerobic anoxygenic phototrophic (AAP) bacteria, which is a strictly aerobic bacterium, producing a photosynthetic pigment bacteriochlorophyll (BChl) a. However, a part of the genus Jannaschia members have not been confirmed the photosynthetic ability. The partly presence of the ability in the genus Jannaschia could suggest the complexity of evolutionary history for anoxygenic photosynthesis in the genus, which is expected as gene loss and/or horizontal gene transfer. Here a novel AAP...
Source: Antonie van Leeuwenhoek - April 24, 2024 Category: Microbiology Authors: Koyo Kuwata Yuki Sato-Takabe Ryosuke Nakai Yuya Sugimura Nozomi Tazato Tadao Kunihiro Sho Morohoshi Mitsunori Iwataki Koji Hamasaki Takuhei Shiozaki Source Type: research
Dietary lipid sensing through fatty acid oxidation and chylomicron formation in the gastrointestinal tract of rainbow trout
This study, using rainbow trout (Oncorhynchus mykiss) as model, aimed to characterize the role of FAO and chylomicron formation as peripheral lipid sensors potentially able to modulate feeding in fish. Fish fed with either a normal- (24%) or high- (32%) fat diet were intraperitoneally injected with water alone or containing etomoxir (inhibitor of FAO rate-limiting enzyme carnitine palmitoyl-transferase 1). First, feed intake levels were recorded. We observed an etomoxir-derived decrease in feeding at short times, but a significant increase at 48 h after treatment in fish fed normal-fat diet. Then, we evaluated putative eto...
Source: Comparative Biochemistry and Physiology. Part A, Molecular and integrative physiology. - April 24, 2024 Category: Physiology Authors: Jessica Calo Ayel én M Blanco Jos é L Soengas Source Type: research
GSE264348 Gene expression profiling of mouse embryonic stem cells with single-cell RNA sequencing
Contributors : Óscar García-Blay ; Maike HansenSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusDifferences in gene-expression profiles between individual cells can give rise to distinct cell fate decisions. Yet how localisation on a micropattern impacts initial changes in mRNA, protein, and phosphoprotein abundance remains unclear. To identify the effect of cellular position on gene expression, we developed a scalable antibody and mRNA targeting sequential fluorescence in situ hybridization (ARTseq-FISH) method capable of simultaneously profiling mRNAs, proteins, and phosphoprotei...
Source: GEO: Gene Expression Omnibus - April 24, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research
Genes, Vol. 15, Pages 534: Population Genomics of Commercial Fish Sebastes schlegelii of the Bohai and Yellow Seas (China) Using a Large SNP Panel from GBS
In this study, a total of 157,778, 174,480, and 188,756 single-nucleotide polymorphisms from Dalian (DL), Yantai (YT), and Qingdao (QD) coastal waters of China were, respectively, identified. Sixty samples (twenty samples per population) were clustered together, indicating shallow structures and close relationships with each other. The observed heterozygosity, expected heterozygosity, polymorphism information content, and nucleotide diversity ranged from 0.14316 to 0.17684, from 0.14035 to 0.17145, from 0.20672 to 0.24678, and from 7.63 × 10−6 to 8.77 × 10−6, respecti...
Source: Genes - April 24, 2024 Category: Genetics & Stem Cells Authors: Beiyan Zhu Tianxiang Gao Yan He Yinquan Qu Xiumei Zhang Tags: Article Source Type: research
A point-of-care testing assay for clonorchiasis using a EuNPs-CsTR1 fluorescent probe-based immunoassay
In this study, Eu-(III) nanoparticles (EuNPs) were used as indicative probes, combined withC.sinensis tandem repeat sequence 1 (CSTR1) antigen to capture specific antibodies. Afterward, the complex binds to mouse anti-human IgG immobilized on the test line (T-line) producing a fluorescent signal under UV light. The EuNPs-fluorescent immunoassay (EuNPs-FIA) was successfully constructed, allowing sample detection within 10 min. It enabled both qualitative determination with the naked eye under UV light and quantitative detection by scanning the fluorescence intensity on the test line and control line (C-line). A total of 133...
Source: PLoS Neglected Tropical Diseases - April 24, 2024 Category: Tropical Medicine Authors: Xiaoxiao Ma Source Type: research
circ_JMJD1C expedites breast cancer progression by regulating miR-182-5p/JMJD1C/SOX4 axis
This study aimed to investigate the roles of circ_0006743 (circ_JMJD1C) in breast cancer. The downstream of circ_JMJD1C and their interaction network was determined by bioinformatic analyses. Gene expression were analyzed through western blot and qRT-PCR assays. Functional assays were conducted in vitro and in vivo to verify circ_JMJD1C role in BC. FISH and confocal analysis indicated the cellular distribution of circ_JMJD1C. Luciferase reporter, RNA immune-precipitation (RIP) assays, as well as Pearson's correlation analysis, were implemented to test the relation of miR-182-5p, JMJD1C and circ_JMJD1C. Circ_JMJD1C and JMJD...
Source: Cellular and Molecular Biology - April 23, 2024 Category: Molecular Biology Authors: Renyuan Xu Haomiao Lan Li Zhang Sisi Yang Yu Mao Hongying Che Source Type: research
The associations of fatty acids related dietary patterns with overweight and obesity among Chinese children
CONCLUSION: Four FAs related dietary patterns were identified. The dietary pattern with high intake of fish, shrimp, crab and shellfish decreased overweight risk by increasing n-3 PUFAs, and decreasing SFAs. The dietary pattern with high intake of plant food, decreased obesity risk by providing an balanced n-6/n-3 PUFAs ratio.PMID:38654346 | DOI:10.1186/s41043-024-00549-9 (Source: Journal of Health, Population, and Nutrition)
Source: Journal of Health, Population, and Nutrition - April 23, 2024 Category: International Medicine & Public Health Authors: Zhi Huang Keyu Ma Xiaochen Yin Ziming Li Ming Chen Yujie Duan Li Li Yuming Hu Source Type: research
Genome-wide DNA methylation changes in Oryzias melastigma embryos exposed to the water accommodated fraction of crude oil
Ecotoxicol Environ Saf. 2024 Apr 22;277:116325. doi: 10.1016/j.ecoenv.2024.116325. Online ahead of print.ABSTRACTThe water accommodated fraction (WAF) of crude oil exerts considerable impacts on marine fish during embryonic stage. Clarifying changes in epigenetic modifications is helpful for understanding the molecular mechanism underlying the toxicity of embryonic WAF exposure. The aim of this study was to explore genome-wide DNA methylation changes in Oryzias melastigma embryos after exposure to the nominal total petroleum hydrocarbon concentration of 500 μg/L in WAF for 7 days. Whole-genome bisulfite sequencing reveale...
Source: Ecotoxicology and Environmental Safety - April 23, 2024 Category: Environmental Health Authors: Chenshi Wang Wei Lei Lichao Du Yiran Xu Yingbin Lou Mo Peng Dongxu Gao Source Type: research
