The role of catechin in electroporation of pancreatic cancer cells - Effects on pore formation and multidrug resistance proteins
Bioelectrochemistry. 2022 Jun 30;147:108199. doi: 10.1016/j.bioelechem.2022.108199. Online ahead of print.ABSTRACTCatechin is a bioflavonoid known for its anti-cancer properties. In the present study, we combined theoretical and experimental approaches to reveal the potential of catechin application in the electroporation (EP) or electrochemotherapy (ECT) of pancreatic cancer cells. The molecular dynamics simulations were implemented to examine the interactions of catechin with a model of a membrane, its influence on the membrane's thickness, and the impact of the catechin-membrane interaction on the pore formation. The da...
Source: Bioelectrochemistry - July 16, 2022 Category: Biochemistry Authors: Olga Michel Wojciech Szlasa Dagmara Baczy ńska Jolanta Saczko Mounir Tarek Julita Kulbacka Source Type: research
Quercetin Inhibits KBM7R Cell Proliferation through Wnt/ < em > β < /em > -Catenin Signaling
CONCLUSIONS: Our findings suggest that Qu could inhibit proliferation, induce apoptosis, and arrest the cell cycle on IM-resistant KBM7R cells with T315I mutation. And this effect could be related with the inhibition of the Wnt/β-catenin signal pathway and downstream targets.PMID:35783534 | PMC:PMC9249478 | DOI:10.1155/2022/1378976 (Source: Evidence-based Complementary and Alternative Medicine)
Source: Evidence-based Complementary and Alternative Medicine - July 5, 2022 Category: Complementary Medicine Authors: Wei Li Yang Yu Huanchen Cheng Shengwei Liu Tiejun Gong Jun Ma Qinghua Tang Source Type: research
Screening of honey bee pollen constituents against COVID-19: an emerging hot spot in targeting SARS-CoV-2-ACE-2 interaction
Nat Prod Res. 2022 Jun 25:1-7. doi: 10.1080/14786419.2022.2092865. Online ahead of print.ABSTRACTThe attachment of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike to angiotensin-converting enzyme 2 (ACE-2) leads the cell fusion process, so spike blockade may be a promising therapy combating COVID-19. Bee pollen bioflavonoids with intrinsic bioactivities are of outmost importance to block SARS-CoV-2-ACE-2 interaction. Herein, we conducted a molecular docking assessment through natural phenolics/non-phenolics of pollen to investigate their affinity against SARS-CoV-2 spike. Finally, kaempferol 3-neohesperi...
Source: Natural Product Research - June 27, 2022 Category: Biochemistry Authors: Armin Zarei Ali Ramazani Aram Rezaei Sajad Moradi Source Type: research
Carbon dioxide expanded liquid: an effective solvent for the extraction of quercetin from South African medicinal plants
Quercetin is one of the most important bioflavonoids having positive effects on the biological processes and human health. Typically, it is extracted from plant matrices using conventional methods such as mace... (Source: Plant Methods)
Source: Plant Methods - June 23, 2022 Category: Biology Authors: Veronika Pila řová, Lukáš Kuda, Hana Kočová Vlčková, Lucie Nováková, Shubhpriya Gupta, Manoj Kulkarni, František Švec, Johannes Van Staden and Karel Doležal Tags: Methodology Source Type: research
α-L-rhamnosidase from Penicillium tardum and Its Application for Biotransformation of Citrus Rhamnosides
Appl Biochem Biotechnol. 2022 Jun 7. doi: 10.1007/s12010-022-04008-1. Online ahead of print.ABSTRACTEnzymatic deramnosylation of flavonoids is a convenient tool for improving the quality of citrus juices. α-L-rhamnosidase with a specific activity of 33.1 units/mg was isolated and characterized from the culture liquid of Penicillium tardum. The molecular weight of the enzyme was 95 kDa according to the data of gel filtration on Sepharose 6B and gel electrophoresis in SDS-PAGE. The pH optimum of the enzyme activity was 5.0, and the thermo optimum was 60 °C. Enzyme showed high stability in the temperature range of 45-50 and...
Source: Applied Biochemistry and Biotechnology - June 7, 2022 Category: Biochemistry Authors: Nataliya Borzova Olena Gudzenko Lyudmila Varbanets Source Type: research
α-L-rhamnosidase from Penicillium tardum and Its Application for Biotransformation of Citrus Rhamnosides
Appl Biochem Biotechnol. 2022 Jun 7. doi: 10.1007/s12010-022-04008-1. Online ahead of print.ABSTRACTEnzymatic deramnosylation of flavonoids is a convenient tool for improving the quality of citrus juices. α-L-rhamnosidase with a specific activity of 33.1 units/mg was isolated and characterized from the culture liquid of Penicillium tardum. The molecular weight of the enzyme was 95 kDa according to the data of gel filtration on Sepharose 6B and gel electrophoresis in SDS-PAGE. The pH optimum of the enzyme activity was 5.0, and the thermo optimum was 60 °C. Enzyme showed high stability in the temperature range of 45-50 and...
Source: Applied Biochemistry and Biotechnology - June 7, 2022 Category: Biochemistry Authors: Nataliya Borzova Olena Gudzenko Lyudmila Varbanets Source Type: research
Regulation of interleukin-6 and matrix metalloproteinases syntheses by bioflavonoids and photobiomodulation in human gingival fibroblasts
In conclusion, TNF-α up-regulated IL-6 and MMPs, while bioflavonoids and PBM down-regulated MMP-2 and MMP-9 syntheses; GSE als o decreased IL-6 synthesis, demonstrating the individual promising potential of these therapies for ulceration management. (Source: Lasers in Medical Science)
Source: Lasers in Medical Science - May 25, 2022 Category: Laser Surgery Source Type: research
Molecules, Vol. 27, Pages 3410: Seed Priming with Devosia sp. Cell-Free Supernatant (CFS) and Citrus Bioflavonoids Enhance Canola and Soybean Seed Germination
Smith
Climate change, environmental pollution and associated abiotic stresses are beginning to meaningfully affect agricultural production worldwide. Salt stress is, however, one of the most important threats that significantly impairs plant growth and development. Plants in their early growth stages such as seed germination, seed emergence and early seedling growth are very sensitive to salt stress. Among the range of sustainable techniques adopted to improve seed germination and early plant growth is seed priming; however, with the use of ecofriendly substances, this is one of the most effective and economically viab...
Source: Molecules - May 25, 2022 Category: Chemistry Authors: Ateeq Shah Sowmyalakshmi Subramanian Donald L. Smith Tags: Article Source Type: research
Quercetin ameliorates oxidative stress ‑induced cell apoptosis of seminal vesicles via activating Nrf2 in type 1 diabetic rats
In this study, we explore the effect of quercetin on cell apoptosis of seminal vesicles and its underlying mechanism. The STZ-induced type 1 diabetic rat model was established. Three doses (low, medium and high) of quercetin were administrated to the STZ-induced type 1 diabetic rats for 4 months. Fasting blood glucose, the fructose in seminal plasma, total antioxidant capacity (T-AOC) and malondialdehyde (MDA) in seminal vesicles were determined by colorimetric method. Nuclear transcription factor- Nrf2 was observed by immunofluorescent staining. Biomarkers related to cell apoptosis, such as Bcl-2, Bax and cleaved -Caspase...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - May 20, 2022 Category: Drugs & Pharmacology Authors: Bingzheng Dong Zhenduo Shi Yang Dong Jiangang Chen Zhuo-Xun Wu Wei Wu Zhe-Sheng Chen Conghui Han Source Type: research
Quercetin ameliorates oxidative stress ‑induced cell apoptosis of seminal vesicles via activating Nrf2 in type 1 diabetic rats
In this study, we explore the effect of quercetin on cell apoptosis of seminal vesicles and its underlying mechanism. The STZ-induced type 1 diabetic rat model was established. Three doses (low, medium and high) of quercetin were administrated to the STZ-induced type 1 diabetic rats for 4 months. Fasting blood glucose, the fructose in seminal plasma, total antioxidant capacity (T-AOC) and malondialdehyde (MDA) in seminal vesicles were determined by colorimetric method. Nuclear transcription factor- Nrf2 was observed by immunofluorescent staining. Biomarkers related to cell apoptosis, such as Bcl-2, Bax and cleaved -Caspase...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - May 20, 2022 Category: Drugs & Pharmacology Authors: Bingzheng Dong Zhenduo Shi Yang Dong Jiangang Chen Zhuo-Xun Wu Wei Wu Zhe-Sheng Chen Conghui Han Source Type: research
Neuroprotective Effect of Baicalein Against Oxaliplatin-Induced Peripheral Neuropathy: Impact on Oxidative Stress, Neuro-inflammation and WNT/ β-Catenin Signaling
This study was designed to investigate the possible neuroprotective potential of a bioflavonoid, baicalein in an experimental model of oxaliplatin-induced peripheral n europathy. Rats were administered with a dose of 4 mg/kg oxaliplatini.p. twice per week for four weeks, and were evaluated for behavioral and functional nerve parameters, followed by biochemical, immunohistochemical and western blot analysis. This study shows that baicalein reversed oxaliplatin-induced behavioral deficits and significantly prevented oxaliplatin-induced sensory nerve conduction deficits in rats. Molecular analysis revealed baicalein signific...
Source: Molecular Neurobiology - May 9, 2022 Category: Neurology Source Type: research
Apigenin suppresses tumor angiogenesis and growth via inhibiting HIF-1 α expression in non-small cell lung carcinoma
Chem Biol Interact. 2022 May 2:109966. doi: 10.1016/j.cbi.2022.109966. Online ahead of print.ABSTRACTTumor angiogenesis inhibitors such as Bevacizumab, Ramucirumab and Endostar have been applied to the therapy of non-small cell lung carcinoma (NSCLC) patients, especially for lung adenocarcinoma (LUAD). However, several safe concerns such as neutropenia, febrile neutropenia and hypertension pulmonary hemorrhage limit their further development. And they often showed poor efficacy and serious side effect for lung squamous cell carcinoma (LUSC) patient. Thus, identification of effective and safe tumor angiogenesis inhibitor fo...
Source: Chemico-Biological Interactions - May 5, 2022 Category: Molecular Biology Authors: Jijun Fu Wenjuan Zeng Minshan Chen Lijuan Huang Songpei Li Zhan Li Qianrong Pan Sha Lv Xiangyu Yang Ying Wang Mengmeng Yi Jianye Zhang Xueping Lei Source Type: research
Apigenin suppresses tumor angiogenesis and growth via inhibiting HIF-1 α expression in non-small cell lung carcinoma
Chem Biol Interact. 2022 May 2:109966. doi: 10.1016/j.cbi.2022.109966. Online ahead of print.ABSTRACTTumor angiogenesis inhibitors such as Bevacizumab, Ramucirumab and Endostar have been applied to the therapy of non-small cell lung carcinoma (NSCLC) patients, especially for lung adenocarcinoma (LUAD). However, several safe concerns such as neutropenia, febrile neutropenia and hypertension pulmonary hemorrhage limit their further development. And they often showed poor efficacy and serious side effect for lung squamous cell carcinoma (LUSC) patient. Thus, identification of effective and safe tumor angiogenesis inhibitor fo...
Source: Chemico-Biological Interactions - May 5, 2022 Category: Molecular Biology Authors: Jijun Fu Wenjuan Zeng Minshan Chen Lijuan Huang Songpei Li Zhan Li Qianrong Pan Sha Lv Xiangyu Yang Ying Wang Mengmeng Yi Jianye Zhang Xueping Lei Source Type: research
