Development of Plant-Based Larvicide and Herbal Mosquito Repellent Fast Card with Reference to Identification of the Functional Bioactive Compounds Effective Against Culex Mosquito
Appl Biochem Biotechnol. 2022 Jan 26. doi: 10.1007/s12010-022-03798-8. Online ahead of print.ABSTRACTWarm and humid climate creates ideal conditions for mosquito breeding. The ability of these vectors to spread a number of diseases to humans causes millions of deaths every year. Indiscriminate use of synthetic insecticides leads to the development of resistance in vector mosquitoes and along with this, these pesticides cause biological magnification of toxic components and affects adversely the non-target organisms including human being. Commercially available, chemically manufactured mosquito repellent fast cards are conv...
Source: Applied Biochemistry and Biotechnology - January 26, 2022 Category: Biochemistry Authors: Amit Chattopadhyay Vikky Shaw Piyali Mukherjee Subarna Ghosh Pranab Kr Banerjee Source Type: research

A Calibration-Free Measurement for Monitoring Cellular Calcium Transients Adaptively
In this study, we presented a calibration-free method, based on calcium imaging, to calculate cellular calcium transient and correct photobleaching directly from the target video. We also set up image acquisition and calculation system on custom software, applied to calcium transients monitoring of neonatal rat cardiomyocytes. Results showed that the effect of the new method was similar to that of the traditional one with a Pearson correlation coefficient of 0.99 ± 0.01. Moreover, the residual sum of squares of the two methods was only 26.31 ± 26.28 when the area of the region of interest was greater than 8% ...
Source: Applied Biochemistry and Biotechnology - January 23, 2022 Category: Biochemistry Authors: Lu Gao Jing Ye Zhenghua Xiao Ling He Jing Zhang Han Zhang Gang Yang Source Type: research

A Calibration-Free Measurement for Monitoring Cellular Calcium Transients Adaptively
In this study, we presented a calibration-free method, based on calcium imaging, to calculate cellular calcium transient and correct photobleaching directly from the target video. We also set up image acquisition and calculation system on custom software, applied to calcium transients monitoring of neonatal rat cardiomyocytes. Results showed that the effect of the new method was similar to that of the traditional one with a Pearson correlation coefficient of 0.99 ± 0.01. Moreover, the residual sum of squares of the two methods was only 26.31 ± 26.28 when the area of the region of interest was greater than 8% ...
Source: Applied Biochemistry and Biotechnology - January 23, 2022 Category: Biochemistry Authors: Lu Gao Jing Ye Zhenghua Xiao Ling He Jing Zhang Han Zhang Gang Yang Source Type: research

A Calibration-Free Measurement for Monitoring Cellular Calcium Transients Adaptively
In this study, we presented a calibration-free method, based on calcium imaging, to calculate cellular calcium transient and correct photobleaching directly from the target video. We also set up image acquisition and calculation system on custom software, applied to calcium transients monitoring of neonatal rat cardiomyocytes. Results showed that the effect of the new method was similar to that of the traditional one with a Pearson correlation coefficient of 0.99 ± 0.01. Moreover, the residual sum of squares of the two methods was only 26.31 ± 26.28 when the area of the region of interest was greater than 8% ...
Source: Applied Biochemistry and Biotechnology - January 23, 2022 Category: Biochemistry Authors: Lu Gao Jing Ye Zhenghua Xiao Ling He Jing Zhang Han Zhang Gang Yang Source Type: research

Purification and Biochemical Characterization of Two Anionic Peroxidase Isoenzymes from Raphanus sativus L. var niger Roots
Appl Biochem Biotechnol. 2022 Jan 22. doi: 10.1007/s12010-021-03736-0. Online ahead of print.ABSTRACTPeroxidase is a commonly used enzyme with a wide range of applications. Horseradish (Armoracia rusticana) is the most well-known source of peroxidase enzyme. Peroxidases extracted from other plant sources have also been proved as useful, sometimes even superior, comparing to traditional horseradish peroxidase (HRP). In the present study, two novel peroxidase isoenzymes were purified and characterized from Raphanus sativus L. var niger roots. Two anionic peroxidase isoenzymes were purified using diafiltration, ammonium sulfa...
Source: Applied Biochemistry and Biotechnology - January 22, 2022 Category: Biochemistry Authors: Hooman Askari Aliasghar Rahimian Mahdi Aminian Source Type: research

Comparative 16S Metabarcoding of Nile Tilapia Gut Microbiota from the Northern Lakes of Egypt
Appl Biochem Biotechnol. 2022 Jan 20. doi: 10.1007/s12010-021-03750-2. Online ahead of print.ABSTRACTNile tilapia, Oreochromis niloticus, is the principal fish bred in Egypt. A pilot study was designed to analyze the bacterial composition of the Nile tilapia fish guts from two saltwater lakes in Northern Egypt. Fish samples were obtained from two Delta lakes: Manzala (ML) and Borollus (BL). DNA was extracted, and the bacterial communities in the stomach content were classified (down to the species level) using the 16S rRNA-based analysis. From the two metagenomics libraries in this study, 1,426,740 reads of the amplicon se...
Source: Applied Biochemistry and Biotechnology - January 20, 2022 Category: Biochemistry Authors: Ahmed M Serag Mohamed S Abdel-Sabour Mohamed El-Hadidi Mohamad Maged Mahmoud Magdy Mohamed Fawzy Ramadan Mohamed H Refaat Source Type: research

Efficient Valorization of Sugarcane Bagasse into Furfurylamine in Benign Deep Eutectic Solvent ChCl:Gly-Water
Appl Biochem Biotechnol. 2022 Jan 20. doi: 10.1007/s12010-021-03784-6. Online ahead of print.ABSTRACTRecently, highly efficient production of valuable furan-based chemicals from available and renewable lignocellulosic biomass has attracted more and more attention via a chemoenzymatic route in an environmentally friendly reaction system. In this work, the feasibility of chemoenzymatically catalyzing sugarcane bagasse into furfurylamine with heterogeneous catalyst and ω-transaminase biocatalyst was developed in the deep eutectic solvent (DES) ChCl:Gly-water. Sulfonated Al-Laubanite was firstly synthesized to catalyze s...
Source: Applied Biochemistry and Biotechnology - January 20, 2022 Category: Biochemistry Authors: Junhua Di Nana Zhao Bo Fan Yu-Cai He Cuiluan Ma Source Type: research

Arthrospira platensis-Mediated Green Biosynthesis of Silver Nano-particles as Breast Cancer Controlling Agent: In Vitro and In Vivo Safety Approaches
Appl Biochem Biotechnol. 2022 Jan 20. doi: 10.1007/s12010-021-03751-1. Online ahead of print.ABSTRACTBiogenic silver nanoparticles (bio-AgNPs) is one of the most fascinating nanomaterials used for several biomedical purposes. In the current study, we biosynthesized AgNPs (bio-AgNPs) using Arthrospira platensis (A-bio-AgNPs), Microcystis aeruginosa (M-bio-AgNPs), and Chlorella vulgaris (C-bio-AgNPs) active metabolites and evaluated their anticancer efficacy against breast cancer. The recovered bio-AgNPs were characterized using scanning and transmission electron microscopy (SEM and TEM). In addition, their safety profiles w...
Source: Applied Biochemistry and Biotechnology - January 20, 2022 Category: Biochemistry Authors: Nehal M El-Deeb Mai A Abo-Eleneen Omyma A Awad Atef M Abo-Shady Source Type: research

Immobilization of Eversa Lipases on Hydrophobic Supports for Ethanolysis of Sunflower Oil Solvent-Free
Appl Biochem Biotechnol. 2022 Jan 20. doi: 10.1007/s12010-021-03774-8. Online ahead of print.ABSTRACTLipases are an important group of biocatalysts for many industrial applications. Two new commercial low-cost lipases Eversa® Transform and Eversa® Transform 2.0 was immobilized on four different hydrophobic supports: Lewatit-DVB, Purolite-DVB, Sepabeads-C18, and Purolite-C18. The performance of immobilized lipases was investigated in the transesterification of sunflower oil solvent-free in an anhydrous medium. Interesting results were obtained for both lipases and the four supports, but with Sepabeads support the li...
Source: Applied Biochemistry and Biotechnology - January 20, 2022 Category: Biochemistry Authors: Daniela Remonatto J Vladimir Oliveira J Manuel Guisan D ébora Oliveira Jorge Ninow Gloria Fernandez-Lorente Source Type: research

Comparative 16S Metabarcoding of Nile Tilapia Gut Microbiota from the Northern Lakes of Egypt
Appl Biochem Biotechnol. 2022 Jan 20. doi: 10.1007/s12010-021-03750-2. Online ahead of print.ABSTRACTNile tilapia, Oreochromis niloticus, is the principal fish bred in Egypt. A pilot study was designed to analyze the bacterial composition of the Nile tilapia fish guts from two saltwater lakes in Northern Egypt. Fish samples were obtained from two Delta lakes: Manzala (ML) and Borollus (BL). DNA was extracted, and the bacterial communities in the stomach content were classified (down to the species level) using the 16S rRNA-based analysis. From the two metagenomics libraries in this study, 1,426,740 reads of the amplicon se...
Source: Applied Biochemistry and Biotechnology - January 20, 2022 Category: Biochemistry Authors: Ahmed M Serag Mohamed S Abdel-Sabour Mohamed El-Hadidi Mohamad Maged Mahmoud Magdy Mohamed Fawzy Ramadan Mohamed H Refaat Source Type: research

Efficient Valorization of Sugarcane Bagasse into Furfurylamine in Benign Deep Eutectic Solvent ChCl:Gly-Water
Appl Biochem Biotechnol. 2022 Jan 20. doi: 10.1007/s12010-021-03784-6. Online ahead of print.ABSTRACTRecently, highly efficient production of valuable furan-based chemicals from available and renewable lignocellulosic biomass has attracted more and more attention via a chemoenzymatic route in an environmentally friendly reaction system. In this work, the feasibility of chemoenzymatically catalyzing sugarcane bagasse into furfurylamine with heterogeneous catalyst and ω-transaminase biocatalyst was developed in the deep eutectic solvent (DES) ChCl:Gly-water. Sulfonated Al-Laubanite was firstly synthesized to catalyze s...
Source: Applied Biochemistry and Biotechnology - January 20, 2022 Category: Biochemistry Authors: Junhua Di Nana Zhao Bo Fan Yu-Cai He Cuiluan Ma Source Type: research

Arthrospira platensis-Mediated Green Biosynthesis of Silver Nano-particles as Breast Cancer Controlling Agent: In Vitro and In Vivo Safety Approaches
Appl Biochem Biotechnol. 2022 Jan 20. doi: 10.1007/s12010-021-03751-1. Online ahead of print.ABSTRACTBiogenic silver nanoparticles (bio-AgNPs) is one of the most fascinating nanomaterials used for several biomedical purposes. In the current study, we biosynthesized AgNPs (bio-AgNPs) using Arthrospira platensis (A-bio-AgNPs), Microcystis aeruginosa (M-bio-AgNPs), and Chlorella vulgaris (C-bio-AgNPs) active metabolites and evaluated their anticancer efficacy against breast cancer. The recovered bio-AgNPs were characterized using scanning and transmission electron microscopy (SEM and TEM). In addition, their safety profiles w...
Source: Applied Biochemistry and Biotechnology - January 20, 2022 Category: Biochemistry Authors: Nehal M El-Deeb Mai A Abo-Eleneen Omyma A Awad Atef M Abo-Shady Source Type: research

Immobilization of Eversa Lipases on Hydrophobic Supports for Ethanolysis of Sunflower Oil Solvent-Free
Appl Biochem Biotechnol. 2022 Jan 20. doi: 10.1007/s12010-021-03774-8. Online ahead of print.ABSTRACTLipases are an important group of biocatalysts for many industrial applications. Two new commercial low-cost lipases Eversa® Transform and Eversa® Transform 2.0 was immobilized on four different hydrophobic supports: Lewatit-DVB, Purolite-DVB, Sepabeads-C18, and Purolite-C18. The performance of immobilized lipases was investigated in the transesterification of sunflower oil solvent-free in an anhydrous medium. Interesting results were obtained for both lipases and the four supports, but with Sepabeads support the li...
Source: Applied Biochemistry and Biotechnology - January 20, 2022 Category: Biochemistry Authors: Daniela Remonatto J Vladimir Oliveira J Manuel Guisan D ébora Oliveira Jorge Ninow Gloria Fernandez-Lorente Source Type: research

Comparative 16S Metabarcoding of Nile Tilapia Gut Microbiota from the Northern Lakes of Egypt
Appl Biochem Biotechnol. 2022 Jan 20. doi: 10.1007/s12010-021-03750-2. Online ahead of print.ABSTRACTNile tilapia, Oreochromis niloticus, is the principal fish bred in Egypt. A pilot study was designed to analyze the bacterial composition of the Nile tilapia fish guts from two saltwater lakes in Northern Egypt. Fish samples were obtained from two Delta lakes: Manzala (ML) and Borollus (BL). DNA was extracted, and the bacterial communities in the stomach content were classified (down to the species level) using the 16S rRNA-based analysis. From the two metagenomics libraries in this study, 1,426,740 reads of the amplicon se...
Source: Applied Biochemistry and Biotechnology - January 20, 2022 Category: Biochemistry Authors: Ahmed M Serag Mohamed S Abdel-Sabour Mohamed El-Hadidi Mohamad Maged Mahmoud Magdy Mohamed Fawzy Ramadan Mohamed H Refaat Source Type: research

Efficient Valorization of Sugarcane Bagasse into Furfurylamine in Benign Deep Eutectic Solvent ChCl:Gly-Water
Appl Biochem Biotechnol. 2022 Jan 20. doi: 10.1007/s12010-021-03784-6. Online ahead of print.ABSTRACTRecently, highly efficient production of valuable furan-based chemicals from available and renewable lignocellulosic biomass has attracted more and more attention via a chemoenzymatic route in an environmentally friendly reaction system. In this work, the feasibility of chemoenzymatically catalyzing sugarcane bagasse into furfurylamine with heterogeneous catalyst and ω-transaminase biocatalyst was developed in the deep eutectic solvent (DES) ChCl:Gly-water. Sulfonated Al-Laubanite was firstly synthesized to catalyze s...
Source: Applied Biochemistry and Biotechnology - January 20, 2022 Category: Biochemistry Authors: Junhua Di Nana Zhao Bo Fan Yu-Cai He Cuiluan Ma Source Type: research

Arthrospira platensis-Mediated Green Biosynthesis of Silver Nano-particles as Breast Cancer Controlling Agent: In Vitro and In Vivo Safety Approaches
Appl Biochem Biotechnol. 2022 Jan 20. doi: 10.1007/s12010-021-03751-1. Online ahead of print.ABSTRACTBiogenic silver nanoparticles (bio-AgNPs) is one of the most fascinating nanomaterials used for several biomedical purposes. In the current study, we biosynthesized AgNPs (bio-AgNPs) using Arthrospira platensis (A-bio-AgNPs), Microcystis aeruginosa (M-bio-AgNPs), and Chlorella vulgaris (C-bio-AgNPs) active metabolites and evaluated their anticancer efficacy against breast cancer. The recovered bio-AgNPs were characterized using scanning and transmission electron microscopy (SEM and TEM). In addition, their safety profiles w...
Source: Applied Biochemistry and Biotechnology - January 20, 2022 Category: Biochemistry Authors: Nehal M El-Deeb Mai A Abo-Eleneen Omyma A Awad Atef M Abo-Shady Source Type: research

Immobilization of Eversa Lipases on Hydrophobic Supports for Ethanolysis of Sunflower Oil Solvent-Free
Appl Biochem Biotechnol. 2022 Jan 20. doi: 10.1007/s12010-021-03774-8. Online ahead of print.ABSTRACTLipases are an important group of biocatalysts for many industrial applications. Two new commercial low-cost lipases Eversa® Transform and Eversa® Transform 2.0 was immobilized on four different hydrophobic supports: Lewatit-DVB, Purolite-DVB, Sepabeads-C18, and Purolite-C18. The performance of immobilized lipases was investigated in the transesterification of sunflower oil solvent-free in an anhydrous medium. Interesting results were obtained for both lipases and the four supports, but with Sepabeads support the li...
Source: Applied Biochemistry and Biotechnology - January 20, 2022 Category: Biochemistry Authors: Daniela Remonatto J Vladimir Oliveira J Manuel Guisan D ébora Oliveira Jorge Ninow Gloria Fernandez-Lorente Source Type: research

Survival of Microencapsulated Lactococcus lactis Subsp. lactis R7 Applied in Different Food Matrices
Appl Biochem Biotechnol. 2022 Jan 19. doi: 10.1007/s12010-022-03804-z. Online ahead of print.ABSTRACTSurvival of Lactococcus lactis subsp. lactis R7, microencapsulated with whey and inulin, was analyzed when added to blueberry juice, milk, and cream. For 28 days, cell viability was evaluated for storage (4 °C), simulated gastrointestinal tract (GIT), and thermal resistance. All matrices demonstrated high cell concentration when submitted to GIT (11.74 and 12 log CFU mL-1), except for the blueberry juice. The thermal resistance analysis proved the need for microencapsulation, regardless of the food matrix. The results i...
Source: Applied Biochemistry and Biotechnology - January 19, 2022 Category: Biochemistry Authors: Michele Dutra Rosolen Fernanda Weber Bordini Gabriela de Quadros da Luz Patr ícia Silva Diaz Fabricio Rochedo Concei ção Ângela Maria Fiorentini Wladimir Padilha da Silva Simone Pieniz Source Type: research

Survival of Microencapsulated Lactococcus lactis Subsp. lactis R7 Applied in Different Food Matrices
Appl Biochem Biotechnol. 2022 Jan 19. doi: 10.1007/s12010-022-03804-z. Online ahead of print.ABSTRACTSurvival of Lactococcus lactis subsp. lactis R7, microencapsulated with whey and inulin, was analyzed when added to blueberry juice, milk, and cream. For 28 days, cell viability was evaluated for storage (4 °C), simulated gastrointestinal tract (GIT), and thermal resistance. All matrices demonstrated high cell concentration when submitted to GIT (11.74 and 12 log CFU mL-1), except for the blueberry juice. The thermal resistance analysis proved the need for microencapsulation, regardless of the food matrix. The results i...
Source: Applied Biochemistry and Biotechnology - January 19, 2022 Category: Biochemistry Authors: Michele Dutra Rosolen Fernanda Weber Bordini Gabriela de Quadros da Luz Patr ícia Silva Diaz Fabricio Rochedo Concei ção Ângela Maria Fiorentini Wladimir Padilha da Silva Simone Pieniz Source Type: research

Caffeic Acid Phenethyl Ester Attenuates Dextran Sulfate Sodium-Induced Ulcerative Colitis Through Modulation of NF- κB and Cell Adhesion Molecules
Appl Biochem Biotechnol. 2022 Jan 18. doi: 10.1007/s12010-021-03788-2. Online ahead of print.ABSTRACTUlcerative colitis (UC) is a serious health condition and defined as inflammation in the colon. Untreated, UC can develop into colitis-associated cancer (CAC), for which effective medicines are not available. Natural products are a better choice to treat UC by alleviating the inflammation. Caffeic acid phenethyl ester (CAPE) is a phenolic compound and known for its beneficial effects, including antibacterial, anti-inflammatory, anti-diabetic, and anticancer. We aimed to study the effect of CAPE on dextran sulfate sodium (DS...
Source: Applied Biochemistry and Biotechnology - January 18, 2022 Category: Biochemistry Authors: Ashok Kumar Pandurangan Nooshin Mohebali Mohadeseh Hasanpourghadi Norhaizan Mohd Esa Source Type: research

Isolation and Characterization of Antimicrobial Peptides Isolated from Fagonia bruguieri
This study paves a way to the effective antimicrobials from the plant resources.PMID:35041129 | DOI:10.1007/s12010-022-03818-7 (Source: Applied Biochemistry and Biotechnology)
Source: Applied Biochemistry and Biotechnology - January 18, 2022 Category: Biochemistry Authors: Khamis Sulaiman Al-Dhafri Chai Lay Ching Source Type: research

Myrobalan-Mediated Nanocolloids in Controlling Marine Pathogens
Appl Biochem Biotechnol. 2022 Jan 17. doi: 10.1007/s12010-022-03816-9. Online ahead of print.ABSTRACTAquaculture production is affected by disease outbreak, which affects the production, profitability, and sustainability of the global aquaculture industry. Antibiotics have been widely used to control various infectious diseases. Indiscriminate usage of antibiotics results in development of antibiotic resistance in pathogens. This current study aims to synthesize myrobalan-mediated green silver nanocolloids (MBNc) by using the extract of three myrobalans and characterized by using various physiochemical techniques. Antibact...
Source: Applied Biochemistry and Biotechnology - January 17, 2022 Category: Biochemistry Authors: S Ranjani Pradeep Parthasarathy P Rameshkumar S Hemalatha Source Type: research

Nematodes as Ghosts of Land Use Past: Elucidating the Roles of Soil Nematode Community Studies as Indicators of Soil Health and Land Management Practices
Appl Biochem Biotechnol. 2022 Jan 17. doi: 10.1007/s12010-022-03808-9. Online ahead of print.ABSTRACTSoil health is a matter of growing concern because of its degradation due to unsustainable anthropogenic activities over the last few decades. It is maintained by interactions among the components of the soil food web commonly concentrated in the vicinity of the plant roots, called the rhizosphere. The soil food web is dominated by nematodes. They occupy various trophic positions because of their diverse feeding habits. The free-living forms are mainly dependent on soil bacteria and fungi for their nutrition, while the para...
Source: Applied Biochemistry and Biotechnology - January 17, 2022 Category: Biochemistry Authors: Debraj Biswal Source Type: research

Myrobalan-Mediated Nanocolloids in Controlling Marine Pathogens
Appl Biochem Biotechnol. 2022 Jan 17. doi: 10.1007/s12010-022-03816-9. Online ahead of print.ABSTRACTAquaculture production is affected by disease outbreak, which affects the production, profitability, and sustainability of the global aquaculture industry. Antibiotics have been widely used to control various infectious diseases. Indiscriminate usage of antibiotics results in development of antibiotic resistance in pathogens. This current study aims to synthesize myrobalan-mediated green silver nanocolloids (MBNc) by using the extract of three myrobalans and characterized by using various physiochemical techniques. Antibact...
Source: Applied Biochemistry and Biotechnology - January 17, 2022 Category: Biochemistry Authors: S Ranjani Pradeep Parthasarathy P Rameshkumar S Hemalatha Source Type: research

Nematodes as Ghosts of Land Use Past: Elucidating the Roles of Soil Nematode Community Studies as Indicators of Soil Health and Land Management Practices
Appl Biochem Biotechnol. 2022 Jan 17. doi: 10.1007/s12010-022-03808-9. Online ahead of print.ABSTRACTSoil health is a matter of growing concern because of its degradation due to unsustainable anthropogenic activities over the last few decades. It is maintained by interactions among the components of the soil food web commonly concentrated in the vicinity of the plant roots, called the rhizosphere. The soil food web is dominated by nematodes. They occupy various trophic positions because of their diverse feeding habits. The free-living forms are mainly dependent on soil bacteria and fungi for their nutrition, while the para...
Source: Applied Biochemistry and Biotechnology - January 17, 2022 Category: Biochemistry Authors: Debraj Biswal Source Type: research

Hydroxyapatite/Glycyrrhizin/Lithium-Based Metal-Organic Framework (HA/GL/Li-MOF) Nanocomposite as Support for Immobilization of Thermomyces lanuginosus Lipase
Appl Biochem Biotechnol. 2022 Jan 15. doi: 10.1007/s12010-022-03800-3. Online ahead of print.ABSTRACTThe hydroxyapatite/glycyrrhizin/lithium-based metal-organic framework (HA/GL/Li-MOF) nanocomposites were synthesized via the hydrothermal method in the presence of lecithin and glycyrrhizin. Fourier transform infrared (FTIR) spectroscopy, thermogravimetric analysis (TGA), and scanning electron microscopy (SEM) equipped with energy-dispersive X-ray spectroscopy (EDS) were applied for characterization of the fabricated nanocomposites. The HA/GL/Li-MOF and Li-MOF nanocomposites were employed as support for immobilization of Th...
Source: Applied Biochemistry and Biotechnology - January 15, 2022 Category: Biochemistry Authors: Atefeh Ameri Fahimeh Asadi Mojtaba Shakibaie Alieh Ameri Hamid Forootanfar Mehdi Ranjbar Source Type: research

Hydroxyapatite/Glycyrrhizin/Lithium-Based Metal-Organic Framework (HA/GL/Li-MOF) Nanocomposite as Support for Immobilization of Thermomyces lanuginosus Lipase
Appl Biochem Biotechnol. 2022 Jan 15. doi: 10.1007/s12010-022-03800-3. Online ahead of print.ABSTRACTThe hydroxyapatite/glycyrrhizin/lithium-based metal-organic framework (HA/GL/Li-MOF) nanocomposites were synthesized via the hydrothermal method in the presence of lecithin and glycyrrhizin. Fourier transform infrared (FTIR) spectroscopy, thermogravimetric analysis (TGA), and scanning electron microscopy (SEM) equipped with energy-dispersive X-ray spectroscopy (EDS) were applied for characterization of the fabricated nanocomposites. The HA/GL/Li-MOF and Li-MOF nanocomposites were employed as support for immobilization of Th...
Source: Applied Biochemistry and Biotechnology - January 15, 2022 Category: Biochemistry Authors: Atefeh Ameri Fahimeh Asadi Mojtaba Shakibaie Alieh Ameri Hamid Forootanfar Mehdi Ranjbar Source Type: research

Expression, Purification, and Characterization of the Recombinant, Two-Component, Response Regulator ArlR from Fusobacterium nucleatum
Appl Biochem Biotechnol. 2022 Jan 14. doi: 10.1007/s12010-021-03785-5. Online ahead of print.ABSTRACTFusobacterium nucleatum is associated with the incidence and development of multiple diseases, such as periodontitis and colorectal cancer (CRC). Until now, studies have proved only a few proteins to be associated with such pathogenic diseases. The two-component system is one of the most prevalent forms of bacterial signal transduction related to intestinal diseases. Here, we report a novel, recombinant, two-component, response regulator protein ArlR from the genome of F. nucleatum strain ATCC 25,586. We optimized the expre...
Source: Applied Biochemistry and Biotechnology - January 14, 2022 Category: Biochemistry Authors: Ruochen Fan Zhuting Li Xian Shi Lulu Wang Xuqiang Zhang Yuesheng Dong Chunshan Quan Source Type: research

A Distance-Based Microfluidic Paper-Based Biosensor for Glucose Measurements in Tear Range
Appl Biochem Biotechnol. 2022 Jan 14. doi: 10.1007/s12010-022-03817-8. Online ahead of print.ABSTRACTThe prevalence of diabetes has increased over the past years. Therefore, developing minimally invasive, user-friendly, and cost-effective glucose biosensors is necessary especially in low-income and developing countries. Cellulose paper-based analytical devices have attracted the attention of many researchers due to affordability, not requiring trained personnel, and complex equipment. This paper describes a microfluidic paper-based analytical device (μPAD) for detecting glucose concentration in tear range with the naked...
Source: Applied Biochemistry and Biotechnology - January 14, 2022 Category: Biochemistry Authors: Samira Allameh Mohsen Rabbani Source Type: research

Expression, Purification, and Characterization of the Recombinant, Two-Component, Response Regulator ArlR from Fusobacterium nucleatum
Appl Biochem Biotechnol. 2022 Jan 14. doi: 10.1007/s12010-021-03785-5. Online ahead of print.ABSTRACTFusobacterium nucleatum is associated with the incidence and development of multiple diseases, such as periodontitis and colorectal cancer (CRC). Until now, studies have proved only a few proteins to be associated with such pathogenic diseases. The two-component system is one of the most prevalent forms of bacterial signal transduction related to intestinal diseases. Here, we report a novel, recombinant, two-component, response regulator protein ArlR from the genome of F. nucleatum strain ATCC 25,586. We optimized the expre...
Source: Applied Biochemistry and Biotechnology - January 14, 2022 Category: Biochemistry Authors: Ruochen Fan Zhuting Li Xian Shi Lulu Wang Xuqiang Zhang Yuesheng Dong Chunshan Quan Source Type: research

A Distance-Based Microfluidic Paper-Based Biosensor for Glucose Measurements in Tear Range
Appl Biochem Biotechnol. 2022 Jan 14. doi: 10.1007/s12010-022-03817-8. Online ahead of print.ABSTRACTThe prevalence of diabetes has increased over the past years. Therefore, developing minimally invasive, user-friendly, and cost-effective glucose biosensors is necessary especially in low-income and developing countries. Cellulose paper-based analytical devices have attracted the attention of many researchers due to affordability, not requiring trained personnel, and complex equipment. This paper describes a microfluidic paper-based analytical device (μPAD) for detecting glucose concentration in tear range with the naked...
Source: Applied Biochemistry and Biotechnology - January 14, 2022 Category: Biochemistry Authors: Samira Allameh Mohsen Rabbani Source Type: research

Eminent Industrial and Biotechnological Applications of Laccases from Bacterial Source: a Current Overview
Appl Biochem Biotechnol. 2022 Jan 13. doi: 10.1007/s12010-021-03781-9. Online ahead of print.ABSTRACTLaccases are blue multicopper oxidases that oxidize a wide range of phenolic as well as non-phenolic substrates in the presence or absence of mediators. They occur in various species of bacteria, fungi, insects, and plants; bacterial laccases show high substrate specificity. Bacteria produce these enzymes either extracellularly or intracellularly and exhibit stability to a wide range of pH and temperature. Therefore, they are suitable for various industrial processes such as food, textile, and paper and pulp industry. They ...
Source: Applied Biochemistry and Biotechnology - January 13, 2022 Category: Biochemistry Authors: Fatima Akram Sadia Ashraf Ikram Ul Haq Fatima Iftikhar Shah Amna Aqeel Source Type: research

Eminent Industrial and Biotechnological Applications of Laccases from Bacterial Source: a Current Overview
Appl Biochem Biotechnol. 2022 Jan 13. doi: 10.1007/s12010-021-03781-9. Online ahead of print.ABSTRACTLaccases are blue multicopper oxidases that oxidize a wide range of phenolic as well as non-phenolic substrates in the presence or absence of mediators. They occur in various species of bacteria, fungi, insects, and plants; bacterial laccases show high substrate specificity. Bacteria produce these enzymes either extracellularly or intracellularly and exhibit stability to a wide range of pH and temperature. Therefore, they are suitable for various industrial processes such as food, textile, and paper and pulp industry. They ...
Source: Applied Biochemistry and Biotechnology - January 13, 2022 Category: Biochemistry Authors: Fatima Akram Sadia Ashraf Ikram Ul Haq Fatima Iftikhar Shah Amna Aqeel Source Type: research

Detection of Aspergillus flavus in Wheat Grains Using Anti-mannoprotein (MP1) and Spore Protein Polyclonal Antibodies
Appl Biochem Biotechnol. 2022 Jan 11. doi: 10.1007/s12010-021-03780-w. Online ahead of print.ABSTRACTCell wall mannoprotein (MP1) gene of an aflatoxigenic strain of Aspergillus flavus, isolated from stored wheat grains, was cloned and sequenced. MP1 protein was expressed in E. coli in soluble form and purified. Polyclonal antibodies were raised against recombinant MP1 protein and inactivated spores of this fungus in rabbit and purified by ammonium sulphate precipitation, Protein A sepharose and antigen affinity chromatography. The minimum concentration of purified mycelial or spore proteins that could be detected by ELISA ...
Source: Applied Biochemistry and Biotechnology - January 11, 2022 Category: Biochemistry Authors: Ranjana Kumari Ananta Kumar Ghosh Source Type: research

In Silico Molecular Docking Approach Against Enzymes Causing Alzheimer's Disease Using Borassus flabellifer Linn
In this study, we explore the activity of natural compounds isolated through GC-MS analysis from the haustoria of palmyra palm against two major Alzheimer's disease-causing enzymes, β-secretase and acetylcholinesterase. The binding affinity of these compounds against the target proteins and their pharmacokinetic properties were checked. Among the 37 compounds docked, 5 compounds showed good binding affinity and pharmacokinetic properties. These natural compounds showed a potential as a drug against Alzheimer's disease. Further research is needed to study the synergistic activity of the compounds in live cells.PMID:350...
Source: Applied Biochemistry and Biotechnology - January 11, 2022 Category: Biochemistry Authors: Jason Tom Abraham H Noorul Samsoon Maharifa S Hemalatha Source Type: research

Environmental Distribution, Metabolic Fate, and Degradation Mechanism of Chlorpyrifos: Recent and Future Perspectives
Appl Biochem Biotechnol. 2022 Jan 11. doi: 10.1007/s12010-021-03713-7. Online ahead of print.ABSTRACTPesticides play a significant role in crop production and have become an inevitable part of the modern environment due to their extensive distribution throughout the soil ecosystem. Prophylactic applications of chlorpyrifos (CP) affect soil fertility, modify soil microbial community structure, and pose potential health risks to the nontarget organisms. Bioremediation through microbial metabolism is found to be an ecofriendly and cheaper process for CP removal from the environment. So far, various bacterial and fungal commun...
Source: Applied Biochemistry and Biotechnology - January 11, 2022 Category: Biochemistry Authors: Rahul S Bhende Upasana Jhariya Shweta Srivastava Sakina Bombaywala Sanchita Das Nishant A Dafale Source Type: research

A Novel MIR503HG/miR-497-5p/CCL19 Axis Regulates High Glucose-Induced Cell Apoptosis, Inflammation, and Fibrosis in Human HK-2 Cells
Appl Biochem Biotechnol. 2022 Jan 11. doi: 10.1007/s12010-021-03776-6. Online ahead of print.ABSTRACTLong non-coding RNAs (lncRNAs) play crucial roles in the development of diabetic nephropathy (DN). Here, we explored the activity and mechanism of MIR503 host gene (MIR503HG) in high glucose (HG)-evoked cytotoxicity in HK-2 cells. MIR503HG, microRNA (miR)-497-5p, and C-C motif chemokine ligand 19 (CCL19) were quantified by quantitative real-time PCR (qRT-PCR) and western blot. The direct relationship between miR-497-5p and MIR503HG or CCL19 was confirmed by dual-luciferase reporter and RNA immunoprecipitation (RIP) assays. ...
Source: Applied Biochemistry and Biotechnology - January 11, 2022 Category: Biochemistry Authors: Danping Zhang Xiaoxiao Chen Dan Zheng Source Type: research

Comparison of Enzyme-Linked Lectin Sorbent Assay and Flow Cytometry for Profiling Microbial Glycans
Appl Biochem Biotechnol. 2022 Jan 11. doi: 10.1007/s12010-021-03772-w. Online ahead of print.ABSTRACTThe surface of microorganisms is covered with carbohydrates, which makes them unique, self-sustaining glycan probes. Lectins are able to bind to these probes, and this interaction can be exploited for selecting microorganisms or novel lectins. To examine lectin-microorganism interactions, we have previously developed an enzyme-linked lectin sorbent assay (ELLSA) with whole bacterial cells. To further test the validity of this methodology, here we compare it with flow cytometry. For this purpose, we used biotinylated recombi...
Source: Applied Biochemistry and Biotechnology - January 11, 2022 Category: Biochemistry Authors: Luka Draga čević Zorana Lopandi ć Marija Gavrovi ć-Jankulović Irena Živković Veljko Blagojevi ć Natalija Polovi ć Rajna Mini ć Source Type: research

Valorization of Biomass to Furfural by Chestnut Shell-based Solid Acid in Methyl Isobutyl Ketone-Water-Sodium Chloride System
This study demonstrated an environmentally friendly strategy for efficiently utilizing corncob into furfural by CNS-based heterogeneous chemocatalyst in a green reaction media. Clearly, this newly synthesized biomass-based MC-Sn-CNS catalyst had potential application in the future.PMID:35015216 | DOI:10.1007/s12010-021-03733-3 (Source: Applied Biochemistry and Biotechnology)
Source: Applied Biochemistry and Biotechnology - January 11, 2022 Category: Biochemistry Authors: Jingjian Zha Bo Fan Jiarui He Yu-Cai He Cuiluan Ma Source Type: research

Screening the Efficacy of Melatonin on Neurodegeneration Mediated by Endoplasmic Reticulum Stress, Inflammation, and Oxidative Damage
Appl Biochem Biotechnol. 2022 Jan 11. doi: 10.1007/s12010-022-03814-x. Online ahead of print.ABSTRACTNeurodegeneration may be defined as a clinical condition wherein neurons gradually lose their structural integrity, viability, and functional abilities and the damage inflicted upon the neurons is often irreversible. The various mechanisms that have been observed to contribute to neurodegeneration include aggregation and accumulation of misfolded proteins, impaired autophagy, oxidative damage, neuroinflammation, mitochondrial defects, increased SUMOylation of proteins, impaired unfolded protein response (UPR) pathways, and ...
Source: Applied Biochemistry and Biotechnology - January 11, 2022 Category: Biochemistry Authors: Fathima Hajee Basha None S Hemalatha Source Type: research

Antimicrobial and Antibiofilm Activity of Biosynthesized Silver Nanoparticles Against Beta-lactamase-Resistant Enterococcus faecalis
This study was aimed to fabricate, optimize, characterize, and evaluate the action of silver nanoparticles (AgNPs) against a clinical isolate of Enterococcus faecalis. A combination of cell-free supernatant (C-FS) of the filamentous fungus Fusarium solani and Gram-negative Comamonas aquatica for AgNP formation was proposed; the antigrowth and antibiofilm of AgNPs against E. faecalis harboring blaTEM and blaCTX-M genes were assessed. The ratio of 1:2 v/v (C-FS:AgNO3) at pH 9.0 for 72 h in 1 mM AgNO3 were the optimal conditions for AgNP formation. UV-vis absorption peak appeared at 425 nm and the crystalline nature of synthe...
Source: Applied Biochemistry and Biotechnology - January 11, 2022 Category: Biochemistry Authors: Bikhal Fattah Huner Arif Haider Hamzah Source Type: research

Quantitation of mHLA-DR and nCD64 by Flow Cytometry to Study Dysregulated Host Response: The Use of QuantiBRITE ™ PE Beads and Its Stability
We report the results of our investigation examining the stability of QuantiBRITE PE beads over a period of 4-week post-reconstitution. The data suggest that reconstituted QuantiBRITE PE beads, if stored in dark at 2-8 °C, can be effectively used for up to 2 weeks for determining nCD64 and mHLA-DR antibody bound per cell (ABC) values.PMID:35015219 | PMC:PMC8748532 | DOI:10.1007/s12010-022-03819-6 (Source: Applied Biochemistry and Biotechnology)
Source: Applied Biochemistry and Biotechnology - January 11, 2022 Category: Biochemistry Authors: S Sanju Paresh Jain Veeraraghavan Vishnu Priya Praveen K Varma Ullas Mony Source Type: research

Detection of Aspergillus flavus in Wheat Grains Using Anti-mannoprotein (MP1) and Spore Protein Polyclonal Antibodies
Appl Biochem Biotechnol. 2022 Jan 11. doi: 10.1007/s12010-021-03780-w. Online ahead of print.ABSTRACTCell wall mannoprotein (MP1) gene of an aflatoxigenic strain of Aspergillus flavus, isolated from stored wheat grains, was cloned and sequenced. MP1 protein was expressed in E. coli in soluble form and purified. Polyclonal antibodies were raised against recombinant MP1 protein and inactivated spores of this fungus in rabbit and purified by ammonium sulphate precipitation, Protein A sepharose and antigen affinity chromatography. The minimum concentration of purified mycelial or spore proteins that could be detected by ELISA ...
Source: Applied Biochemistry and Biotechnology - January 11, 2022 Category: Biochemistry Authors: Ranjana Kumari Ananta Kumar Ghosh Source Type: research

In Silico Molecular Docking Approach Against Enzymes Causing Alzheimer's Disease Using Borassus flabellifer Linn
In this study, we explore the activity of natural compounds isolated through GC-MS analysis from the haustoria of palmyra palm against two major Alzheimer's disease-causing enzymes, β-secretase and acetylcholinesterase. The binding affinity of these compounds against the target proteins and their pharmacokinetic properties were checked. Among the 37 compounds docked, 5 compounds showed good binding affinity and pharmacokinetic properties. These natural compounds showed a potential as a drug against Alzheimer's disease. Further research is needed to study the synergistic activity of the compounds in live cells.PMID:350...
Source: Applied Biochemistry and Biotechnology - January 11, 2022 Category: Biochemistry Authors: Jason Tom Abraham H Noorul Samsoon Maharifa S Hemalatha Source Type: research

Environmental Distribution, Metabolic Fate, and Degradation Mechanism of Chlorpyrifos: Recent and Future Perspectives
Appl Biochem Biotechnol. 2022 Jan 11. doi: 10.1007/s12010-021-03713-7. Online ahead of print.ABSTRACTPesticides play a significant role in crop production and have become an inevitable part of the modern environment due to their extensive distribution throughout the soil ecosystem. Prophylactic applications of chlorpyrifos (CP) affect soil fertility, modify soil microbial community structure, and pose potential health risks to the nontarget organisms. Bioremediation through microbial metabolism is found to be an ecofriendly and cheaper process for CP removal from the environment. So far, various bacterial and fungal commun...
Source: Applied Biochemistry and Biotechnology - January 11, 2022 Category: Biochemistry Authors: Rahul S Bhende Upasana Jhariya Shweta Srivastava Sakina Bombaywala Sanchita Das Nishant A Dafale Source Type: research

A Novel MIR503HG/miR-497-5p/CCL19 Axis Regulates High Glucose-Induced Cell Apoptosis, Inflammation, and Fibrosis in Human HK-2 Cells
Appl Biochem Biotechnol. 2022 Jan 11. doi: 10.1007/s12010-021-03776-6. Online ahead of print.ABSTRACTLong non-coding RNAs (lncRNAs) play crucial roles in the development of diabetic nephropathy (DN). Here, we explored the activity and mechanism of MIR503 host gene (MIR503HG) in high glucose (HG)-evoked cytotoxicity in HK-2 cells. MIR503HG, microRNA (miR)-497-5p, and C-C motif chemokine ligand 19 (CCL19) were quantified by quantitative real-time PCR (qRT-PCR) and western blot. The direct relationship between miR-497-5p and MIR503HG or CCL19 was confirmed by dual-luciferase reporter and RNA immunoprecipitation (RIP) assays. ...
Source: Applied Biochemistry and Biotechnology - January 11, 2022 Category: Biochemistry Authors: Danping Zhang Xiaoxiao Chen Dan Zheng Source Type: research

Comparison of Enzyme-Linked Lectin Sorbent Assay and Flow Cytometry for Profiling Microbial Glycans
Appl Biochem Biotechnol. 2022 Jan 11. doi: 10.1007/s12010-021-03772-w. Online ahead of print.ABSTRACTThe surface of microorganisms is covered with carbohydrates, which makes them unique, self-sustaining glycan probes. Lectins are able to bind to these probes, and this interaction can be exploited for selecting microorganisms or novel lectins. To examine lectin-microorganism interactions, we have previously developed an enzyme-linked lectin sorbent assay (ELLSA) with whole bacterial cells. To further test the validity of this methodology, here we compare it with flow cytometry. For this purpose, we used biotinylated recombi...
Source: Applied Biochemistry and Biotechnology - January 11, 2022 Category: Biochemistry Authors: Luka Draga čević Zorana Lopandi ć Marija Gavrovi ć-Jankulović Irena Živković Veljko Blagojevi ć Natalija Polovi ć Rajna Mini ć Source Type: research

Valorization of Biomass to Furfural by Chestnut Shell-based Solid Acid in Methyl Isobutyl Ketone-Water-Sodium Chloride System
This study demonstrated an environmentally friendly strategy for efficiently utilizing corncob into furfural by CNS-based heterogeneous chemocatalyst in a green reaction media. Clearly, this newly synthesized biomass-based MC-Sn-CNS catalyst had potential application in the future.PMID:35015216 | DOI:10.1007/s12010-021-03733-3 (Source: Applied Biochemistry and Biotechnology)
Source: Applied Biochemistry and Biotechnology - January 11, 2022 Category: Biochemistry Authors: Jingjian Zha Bo Fan Jiarui He Yu-Cai He Cuiluan Ma Source Type: research

Screening the Efficacy of Melatonin on Neurodegeneration Mediated by Endoplasmic Reticulum Stress, Inflammation, and Oxidative Damage
Appl Biochem Biotechnol. 2022 Jan 11. doi: 10.1007/s12010-022-03814-x. Online ahead of print.ABSTRACTNeurodegeneration may be defined as a clinical condition wherein neurons gradually lose their structural integrity, viability, and functional abilities and the damage inflicted upon the neurons is often irreversible. The various mechanisms that have been observed to contribute to neurodegeneration include aggregation and accumulation of misfolded proteins, impaired autophagy, oxidative damage, neuroinflammation, mitochondrial defects, increased SUMOylation of proteins, impaired unfolded protein response (UPR) pathways, and ...
Source: Applied Biochemistry and Biotechnology - January 11, 2022 Category: Biochemistry Authors: Fathima Hajee Basha None S Hemalatha Source Type: research

Antimicrobial and Antibiofilm Activity of Biosynthesized Silver Nanoparticles Against Beta-lactamase-Resistant Enterococcus faecalis
This study was aimed to fabricate, optimize, characterize, and evaluate the action of silver nanoparticles (AgNPs) against a clinical isolate of Enterococcus faecalis. A combination of cell-free supernatant (C-FS) of the filamentous fungus Fusarium solani and Gram-negative Comamonas aquatica for AgNP formation was proposed; the antigrowth and antibiofilm of AgNPs against E. faecalis harboring blaTEM and blaCTX-M genes were assessed. The ratio of 1:2 v/v (C-FS:AgNO3) at pH 9.0 for 72 h in 1 mM AgNO3 were the optimal conditions for AgNP formation. UV-vis absorption peak appeared at 425 nm and the crystalline nature of synthe...
Source: Applied Biochemistry and Biotechnology - January 11, 2022 Category: Biochemistry Authors: Bikhal Fattah Huner Arif Haider Hamzah Source Type: research