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Mild γ-Butyrolactone/Water Pretreatment for Highly Efficient Sugar Production from Corn Stover
In this study, γ-butyrolactone/water (GBL/H2O) was explored as a mild, efficient, and cost-effective binary solvent pretreatment to enhance hydrolyzability of corn stover (CS). Key pretreatment parameters-reaction time, temperature, and H2SO4 concentration-were systematically investigated for their effects on the physicochemical properties of CS. Specifically, increased temperature and acid concentration significantly decreased cellulose crystallinity (from 1.39 for untreated CS to 1.04 for CS pretreated by GBL/H2O with 100 mM H2SO4 at 120 °C for 1 h) and promoted lignin removal (47.3% for CS pretreated by GBL/H2O with 1...
Source: Applied Biochemistry and Biotechnology - April 8, 2024 Category: Biochemistry Authors: Yu Yang Xueliang Ma Manzhu Wang Xinyi Ji Long Li Ziyu Liu Jiangyao Wang Yujin Ren Lili Jia Source Type: research

MiR-449b-5p Ameliorates Hypoxia-induced Cardiomyocyte Injury through Activating PI3K/AKT Pathway by Targeting BCL2L13
In this study, AC16 human cardiomyocytes underwent hypoxic conditions for durations of 24, 48, and 72 h. We observed that miR-449b-5p expression was significantly downregulated in hypoxic AC16 cardiomyocytes. Elevating the levels of miR-449b-5p in these cells resulted in enhanced cell survival, diminished release of LDH, and a reduction in cell apoptosis and oxidative stress using CCK-8, LDH assays, flow cytometry, TUNEL staining, and various commercial kits. Conversely, reducing miR-449b-5p levels resulted in the opposite effects. Through bioinformatics analysis and luciferase reporter assays, BCL2-like 13 (BCL2L13) was d...
Source: Applied Biochemistry and Biotechnology - April 6, 2024 Category: Biochemistry Authors: Yang Jiang Zeyan Liu Li Ye Jinglin Cheng Jun Wan Source Type: research

Utilizing a Fe < sub > 3 < /sub > O < sub > 4 < /sub > Magnetite Nanoparticle for Anode Modification in a Microbial Desalination Cell to Treat Saltwater
In this study, a three-chamber microbial desalination cell was developed to treat seawater using a synthesize Fe3O4 magnetite nanoparticle (MNP)-modified anode. The impact of different performance parameters, such as temperature, pH, and concentrations of NPs, has been investigated in order to assess the performance of three-chamber MDCs in terms of energy recovery and salt removal. The evaluation criteria of the system included multiple factors such as chemical oxygen demand (COD), Coulombic efficiency (CE), desalination efficiency, as well as system aspects including voltage generation and power density. The highest COD%...
Source: Applied Biochemistry and Biotechnology - April 4, 2024 Category: Biochemistry Authors: Shruti Singh Ankit Kumar Soumya Pandit Arpita Roy Dibyajit Lahiri Saad Alghamdi Mazen Almehmadi Ahad Amer Alsaiari Mamdouh Allahyani Source Type: research

Novel Insights into Alkyl Polyglucoside Biosurfactant Promoting Anaerobic Dark Fermentation for Hydrogen Production in Sludge
This study innovatively applied the bio-based surfactant alkyl polyglucoside (APG) to enhance the efficiency of dark fermentation for hydrogen production from ES. When the APG content was 100 mg/g (calculated based on total suspended solids), the maximum hydrogen production reached 17.8 mL/g VSS, approximately 3.7 times that in the control group. Mechanistic analysis revealed that APG promoted the release of organic matter from ES. APG also facilitated the release of soluble protein and soluble polysaccharide, increasing the organic matter reduction rate to 34.8%, significantly higher than other groups. APG enhanced the ac...
Source: Applied Biochemistry and Biotechnology - April 3, 2024 Category: Biochemistry Authors: Xuemei Yang Tiantian Yang Yazhou Xu Source Type: research

FOXO3 Inhibits the Cisplatin Resistance and Progression of Melanoma Cells by Promoting CDKN1C Transcription
CONCLUSION: FOXO3 promoted the transcription of CDKN1C, thereby inhibiting the DDP resistance and progression of melanoma cells.PMID:38568329 | DOI:10.1007/s12010-024-04909-3 (Source: Applied Biochemistry and Biotechnology)
Source: Applied Biochemistry and Biotechnology - April 3, 2024 Category: Biochemistry Authors: Chao Yang Zeqiang Yan Zhihua Sun Fen Hu Wei Xu Source Type: research

Introduction to Special Issue Microbial and Molecular Approaches for Environmental Remediation
Appl Biochem Biotechnol. 2024 Apr 2. doi: 10.1007/s12010-024-04918-2. Online ahead of print.NO ABSTRACTPMID:38564112 | DOI:10.1007/s12010-024-04918-2 (Source: Applied Biochemistry and Biotechnology)
Source: Applied Biochemistry and Biotechnology - April 2, 2024 Category: Biochemistry Authors: Kasturi Dutta Angana Sarkar Achlesh Daverey Sanket Joshi Source Type: research