Photosensitizers attenuate LPS-induced inflammation: implications in dentistry and general health
AbstractAntimicrobial photodynamic therapy (aPDT) is a complementary therapeutic modality for periodontal and endodontic diseases, in which Gram-negative bacteria are directly involved. Currently, there are few evidences regarding the effects of aPDT on bacterial components such as lipopolysaccharide (LPS) and it would represent a major step forward in the clinical use of this therapy. In this context, this study aimed to evaluate the efficacy of different photosensitizers (PSs) used in aPDT in LPS inhibition. Four PSs were used in this study: methylene blue (MB), toluidine blue (TBO), new methylene blue (NMB), and curcumi...
Source: Lasers in Medical Science - November 4, 2020 Category: Laser Surgery Source Type: research

Efficacy and safety of 1440-nm Nd:YAG laser on lower face and neck rejuvenation
AbstractReviewing the literatures, we did not found —to our knowledge—any study using the Nd:YAG laser with wavelength 1440 nm in assisted liposuction and rejuvenation of the head and neck region although of the higher absorption rate for fat and water. Evidence-based practice supports the benefits of laser-assisted liposuction over conventional liposuction with skin tightening in selected areas specifically the submental area and the neck. The aim of the work is to evaluate the efficacy and safety of Nd:YAG laser wavelength (WL) 1440 nm on lipolysis of submental area and skin tightening of the lower ...
Source: Lasers in Medical Science - November 3, 2020 Category: Laser Surgery Source Type: research

Effect of different treatments on recurrent aphthous stomatitis: laser versus medication
In this study, 56 patients were randomly assigned to two groups (n = 28). Laser group was treated using diode laser (810 nm, 1.0 W, CW, irradiation time 20 s for 3 applications) once daily for continuous 3 days. Medication group was treated with triamcinolone acetonide 0.1% three times a day until the lesion was healed. Spontaneous and functional pain level on the third day of treatment was significantly less in the laser group. Significant difference was observed with respect to healing time; however, the order of difference is small albeit of statistical significance. Diode laser with the chosen parameters had better eff...
Source: Lasers in Medical Science - November 2, 2020 Category: Laser Surgery Source Type: research

The inflammation and reactive oxygen species regulated by Nrf2 and NF- κB signaling pathways in 630-nm light-emitting diode irradiation treated THP-1 monocytes/macrophages
AbstractBecause of a large number of macrophages and its secreted pro-inflammatory factors in the synovial fluid of patients with rheumatoid arthritis, the present study aimed to investigate the effect and mechanism of 630-nm LED exposure on monocytes/macrophages and its anti-inflammatory effect. The THP-1 monocytes and PMA-induced THP-1 macrophages (THP-1 macrophages) were employed and irradiated by 630-nm LED for different time and times, and then measure the pro-inflammatory cytokines production by RT-qPCR and Milliplex MAP Multiplex assay, the proteins involved in inflammation pathway and reactive oxygen species (ROS) ...
Source: Lasers in Medical Science - October 31, 2020 Category: Laser Surgery Source Type: research

Photosensitizer delivery by fibrin glue: potential for bypassing the blood-brain barrier
This study will look at the efficacy of FG loaded with drugs in mediating both PDT and PCI in inhibiting 3-dimensional tumor spheroid growth in vitro. Experiments were conducted on spheroids comprised of F98 glioma cells using photosensitizer AlPcS2a and chemotherapeutic drug bleomycin (BLM). At 2-, 24-, 48-, and 72-h increments, supernatant covering an FG layer within a well was collected and replaced by fresh medium, then added to spheroid-containing wells, which contained the respective chemicals for PDT and PCI. The wells were then exposed to light treatment from a diode laser, and after, spheroid growth was monitored ...
Source: Lasers in Medical Science - October 30, 2020 Category: Laser Surgery Source Type: research

Efficacy, safety, and perioperative outcomes of holmium laser enucleation of the prostate —a comparison of patients with lower urinary tract symptoms and urinary retention
AbstractHolmium laser enucleation of the prostate (HoLEP) is a valid treatment option to relieve bladder outlet obstruction in patients with large prostate volumes (PV). Its efficacy, tolerability, and safety are comparable to the ones of other laser treatments of the prostate and resection techniques. However, safety and efficacy of HoLEP have not been compared between patients with and without preoperative urinary retention. We included 350 patients (mean age 71.2 years) who had undergone HoLEP due to lower urinary tract symptoms (LUTS) or urinary retention caused by prostatic hyperplasia. We evaluated the differences in...
Source: Lasers in Medical Science - October 30, 2020 Category: Laser Surgery Source Type: research

The feasibility and safety of photoselective vaporization for prostate using a 180-W XPS Greenlight laser in day-surgery pattern in China
AbstractTo evaluate the feasibility and safety of photoselective vaporization for prostate (PVP) with a 180-W XPS Greenlight laser as day surgery in the Chinese population. We retrospectively collected 114 cases undergoing the day surgery PVP and 198 cases undergoing conventional inpatient surgery PVP from April 2017 to March 2020. All patients ’ data including baseline characteristics, peri-operative data, post-operative outcomes, and complications were recorded and evaluated. The day-surgery procedures were successful in 110 patients. There were no significant differences in baseline characteristics in both groups....
Source: Lasers in Medical Science - October 29, 2020 Category: Laser Surgery Source Type: research

Photobiomodulation reduces the impact of radiotherapy on oral health-related quality of life due to mucositis-related symptoms in head and neck cancer patients
AbstractTo assess the effectiveness of photobiomodulation therapy (PBMT) in the oral health-related quality of life (OHRQoL) of patients with head and neck cancer undergoing radiotherapy (RT), using the Oral Health Impact Profile-14 (OHIP-14) and the Patient-Reported Oral Mucositis (OM) Symptoms Scale (PROMS), and to correlate OM degree with the PROMS and OHIP-14 scores. Forty-eight patients undergoing RT for head and neck cancer were randomly assigned into two groups: PBMT group (n = 25) —daily PBMT associated with a preventive oral care program (POCP); and control group (n = 23) —receiving POCP exclusively. O...
Source: Lasers in Medical Science - October 28, 2020 Category: Laser Surgery Source Type: research

A retrospective study of neck rejuvenation using a noninsulated microneedle radiofrequency in Chinese subjects
AbstractLarge-scale long-term retrospective study of noninsulated microneedle radiofrequency treatment on neck rejuvenation is lacking. We conducted a 3-year retrospective observation of subjects treated with noninsulated microneedle radiofrequency (NIMNRF) for neck rejuvenation in Chinese subjects. Of all subjects, 28 subjects (28.6%) received only 1 session of treatment. About 50% subjects achieved ≥ 50% improvement, and average improvements were 37.0% at 3 months follow-up (M3) and 41.6% at 6 months follow-up (M6). Seventy subjects (71.4%) accepted 3 sessions of treatments. The neck aging scores were...
Source: Lasers in Medical Science - October 28, 2020 Category: Laser Surgery Source Type: research

Proposed mechanisms of low-level light therapy in the treatment of androgenetic alopecia
AbstractAndrogenetic alopecia (AGA) is a global challenge, affecting a large number of people worldwide. Efficacy of the existed treatments can barely meet the demands of patients. Patients who are poorly responding to those treatments are seeking for a more effective and suitable technique to treat their disease. Low-level light therapy (LLLT) is a newly developed technique, which has been proved to stimulate hair growth. Based on the function principle of LLLT in other domains and refer to the published literatures, we write this review to neaten and elucidate the possible mechanism of LLLT in the treatment of AGA. A rev...
Source: Lasers in Medical Science - October 28, 2020 Category: Laser Surgery Source Type: research

Safety of laser-generated shockwave treatment for bacterial biofilms in a cutaneous rodent model
In this study, we aim to determine the safety threshold of the LGS technology in an in vivo rodent model. To understand the in vivo effects of LGS on healthy cutaneous tissue, the de-haired dorsal skin of Sprague-Dawley rats were treated with LGS at three different peak pressures (118, 296, 227 MPa). These pressures were generated using a 1064 nm Nd/YAG laser (pulse duration 5 ns and laser fluence of 777.9 mJ) with laser spot size diameters of 2.2, 3.0, and 4.2 mm, respectively. Following treatment, the animals were observed for 72 h, and a small subset was euthanized at 1-h, 24-h, and 72-h post-treatment and assessed for ...
Source: Lasers in Medical Science - October 27, 2020 Category: Laser Surgery Source Type: research

Effects of photobiomodulation and swimming on gene expression in rats with the tibialis anterior muscle injury
AbstractThe aim of the present study was to evaluate the effects of photobiomodulation (low-level laser therapy (LLLT)) and aquatic exercise on the expression of genes related to muscle regeneration in rats. Wistar rats were divided into five groups: control group (n = 15), non-treated injury group (n = 15), injury+LLLT group (n = 15), injury+aquatic exercise group (n = 15), and injury+LLLT+aquatic exercise group (n = 15). Cryoinjury was performed on the belly of the tibialis anterior (TA) muscle. LLLT was performed daily with an AlGaAs laser (830 nm; beam spot of 0.0324 cm2, output power of 100 mW, energy density of 180 J...
Source: Lasers in Medical Science - October 27, 2020 Category: Laser Surgery Source Type: research

Hypothetical mechanism of light action on nitric oxide physiological effects
AbstractNitric oxide is a universal cellular mediator. It is involved in many physiological processes, including those induced by light. The disability for complete analysis of the nitric oxide metabolites in tissues prevents the exact understanding of the role of NO in a particular process. The sensitivity and selectivity of an enzymatic sensor developed in our lab is based on the detection of all NO groups that carry a positive charge or acquire it in the chemical processes. Using this sensor, we have shown that dinitrosyl iron complexes (DNIC), being principal nitric oxide donors in most of living tissues, undergo trans...
Source: Lasers in Medical Science - October 26, 2020 Category: Laser Surgery Source Type: research

Ablative laser surgery for allergic tattoo reactions: a retrospective study
AbstractPatients with allergic tattoo reactions are burdened with itch and have a reduced quality of life. Conservative treatment is often insufficient and little is known about treatment options to remove the responsible allergen. We aimed to address the effectiveness and safety of ablative laser therapy including measurement of patient ’s satisfaction, in patients with allergic reactions to tattoos. A retrospective study was conducted including patients with allergic tattoo reactions who were treated with a 10,600 nm ablative CO2 laser, either by full-surface ablation or fractional ablation. Clinical informati...
Source: Lasers in Medical Science - October 26, 2020 Category: Laser Surgery Source Type: research

Antimicrobial photodynamic therapy against Propionibacterium acnes biofilms using hypericin ( Hypericum perforatum ) photosensitizer: in vitro study
AbstractAcne vulgaris is the most recurring skin condition in the world, causing great harm to the physical and psychological well-being of many patients. Antimicrobial photodynamic therapy (aPDT) has broad therapeutic applicability. The purpose was to evaluate in vitro the photodynamic inactivation againstPropionibacterium acnes (P. acnes) biofilms by using different concentrations of hypericin (Hypericum perforatum) photosensitizer associated with different energies of low-level laser. The biofilms were placed in 96-well microplates with a 6.4-mm diameter surface, by using standard suspensions (2  × 1...
Source: Lasers in Medical Science - October 21, 2020 Category: Laser Surgery Source Type: research

Metagenomic analysis of the dust particles collected from the suction tube and the suction funnel of a dermatological laser smoke evacuator system
AbstractIn the last few decades, there has essentially been an explosion in the use of lasers in medicine, especially in the area of cosmetic dermatology. Potentially harmful substances are liberated when tissues are vaporized with laser. This creates numerous risks, including the spread of infectious disease. Smoke evacuators are devices that capture and filter laser plume, thereby maintaining a safe environment for the surgical team and patient. Our aim was to characterize the microbial community structure within the suction tube and funnel of the smoke evacuator system, identify their origin, and evaluate pathogenicity....
Source: Lasers in Medical Science - October 20, 2020 Category: Laser Surgery Source Type: research

Comparison of low-level light therapy and combination therapy of 5% minoxidil in the treatment of female pattern hair loss
This study was conducted in order to compare the safety and efficacy of LLLT, 5% minoxidil, and combination therapy in the treatment of FPHL. A randomized controlled trial was developed to study the effect of LLLT on FPHL using a device called iHelmet ®, which is equipped with 200 5mW laser diode source (650 nm) arrays. Ninety Ludwig’s types II–III FPHL patients were randomly divided into 3 groups: LLLT group (A), 5% minoxidil group (B), and combination group(C). Hair density, hair diameter, and scalp oil-secretion were detected to evaluate the treatment effect. Significant improvement of hair density ...
Source: Lasers in Medical Science - October 17, 2020 Category: Laser Surgery Source Type: research

Reflectance confocal microscopy evaluation of pigmented lesions on tattooed skin
AbstractThe evaluation of pigmented lesions on tattooed skin poses a diagnostic challenge for dermatologists, as a nevus may be partially or completely obscured by tattoo pigment. Because of incidences of melanoma arising from tattooed skin, the current gold standard is to biopsy these lesions. Reflectance confocal microscopy (RCM) is a noninvasive imaging modality used in the diagnosis and management of skin diseases that may allow for diagnosis, while preserving the tattoo design. Retrospective chart review was conducted to identify pigmented lesions on or near tattooed skin that were evaluated with RCM. Confocal charact...
Source: Lasers in Medical Science - October 17, 2020 Category: Laser Surgery Source Type: research

Carbon dioxide (CO 2 ) laser ablation of symptomatic vaginal endometriosis: a pilot study
AbstractDeep dyspareunia affects almost half of the women with endometriosis and is associated with vaginal endometriotic lesions. Our pilot study ’s objective is to assess the feasibility and effectiveness of CO2-laser ablation under colposcopic guidance for the treatment of symptomatic vaginal endometriosis. A non-comparative pilot study has been performed. Only women with histologically proven vaginal endometriosis, who declared the presence of moderate or severe deep dyspareunia, resistant to at least 6  months of conventional hormonal treatment, were deemed eligible for the study. All treatments were perfor...
Source: Lasers in Medical Science - October 16, 2020 Category: Laser Surgery Source Type: research

The effect of combined curcumin-mediated photodynamic therapy and artificial skin on Staphylococcus aureus –infected wounds in rats
This study evaluated the effects of antimicrobial photodynamic therapy (aPDT) combined with artificial skin on disinfection of infected skin wound in rats. Twenty-four Wistar rats were randomly distributed into 4 groups (n = 6): (i) control —untreated; (ii) aPDT—treated with curcumin-mediated aPDT (blue light); (iii) artificial skin—treated with artificial skin alcohol-based; and (iv) aPDT plus artificial skin—treated with aPDT associated with artificial skin alcohol-based. For the in vivo model, a full-thickness biopsy with 0. 80 cm was performed in order to inoculate the microorganismStaphylococcu...
Source: Lasers in Medical Science - October 16, 2020 Category: Laser Surgery Source Type: research

Transurethral laser versus open simple prostatectomy for large volume prostates: a systematic review and meta-analysis of randomized controlled trials
AbstractThe benefit of transurethral laser prostatectomy over open simple prostatectomy (OSP) is controversial in aged symptomatic benign prostatic hyperplasia (BPH) patients with large volume prostates, and the aim of this study is to compare the safety and efficiency of these two methods. Meta-analysis was applied using the Review Manager V5.3 software and the retrieved randomized controlled clinical trials (RCTs) comparing transurethral laser prostatectomy with OSP were analyzed for the treatment of large volume prostates from 2000 to 2019 in PubMed, Web of Science, Cochrane, and EMBASE datasets. Five RCTs assessing tra...
Source: Lasers in Medical Science - October 12, 2020 Category: Laser Surgery Source Type: research

Photobiomodulation therapy on expression of HSP70 protein and tissue repair in experimental acute Achilles tendinitis
AbstractThe aim of the present study was to investigate the effects of photobiomodulation (PBM) therapy on the expression of heat shock protein 70 (HSP70) and tissue repair in an experimental model of collagenase-induced Achilles tendinitis. Thirty Wistar rats (aged 12  weeks) were randomly distributed among control group (n = 8), tendinitis group (n = 11), and LED group (n = 11). Tendinitis was induced in the tendinitis and LED groups through a peritendinous injection of collagenase (100 μl). The LED group animals received the first irradiation 1 h after injury. ...
Source: Lasers in Medical Science - October 10, 2020 Category: Laser Surgery Source Type: research

Clinical effectiveness of Er,Cr:YSGG lasers in non-surgical treatment of chronic periodontitis: a meta-analysis of randomized controlled trials
AbstractThe meta-analysis aimed to systematically evaluate all the available pieces of evidence concerning the clinical effectiveness of Er,Cr:YSGG lasers (erbium, chromium, yttrium scandium gallium garnet laser) in the non-surgical treatment of patients with chronic periodontitis, and provide guidance for clinicians about the application of Er,Cr:YSGG lasers during the process of non-surgical periodontal treatments. The meta-analysis was conducted with data extracted from 16 randomized controlled clinical trials (RCTs) that compare Er,Cr:YSGG lasers adjunct/substitute to scaling and root planing (SRP) with SRP alone for t...
Source: Lasers in Medical Science - October 9, 2020 Category: Laser Surgery Source Type: research

Photobiomodulation of avian embryos by red laser
AbstractThe current research focuses on the effect of variable doses of red laser on the chick embryonic development. He-Ne laser of 632-nm wavelength was used as an irradiation source in the first 48 h post-laying of chicken eggs. We have used five different doses: 2, 1, 0.3, 0.2, and 0.1 mJ/cm2 that needed a time range for about 400 –20 s. Those irradiated embryos were left for additional 11 days for incubation in normal conditions, where they are blindly studied after the 11th day. Light microscopy was used in this study to investigate the histological and pathological features of the different experimental groups...
Source: Lasers in Medical Science - October 3, 2020 Category: Laser Surgery Source Type: research

Photobiomodulation can prevent apoptosis in cells from mouse periodontal ligament
AbstractPhotobiomodulation (PBM) has been used to modulate the inflammatory and immune responses, pain relief, and to promote wound healing. PBM is widely used in dental practice and its cellular effects should be investigated. The aim was to evaluate if PBM changes proteins cell death –related, such as caspase-6 and Bcl-2, in periodontal ligament cells. Eighteen mice were divided in three groups (n = 6), i.e., (I) control, (II) 3 J cm−2, and (III) 30 J cm−2. Low power infrared laser (830 nm) parameters were power at 10 mW, energy densities at 3 and 30 J cm−2 in continuous emission mode, exposure ti...
Source: Lasers in Medical Science - September 22, 2020 Category: Laser Surgery Source Type: research

Prophylactic photobiomodulation therapy using 660 nm diode laser for oral mucositis in paediatric patients under chemotherapy: 5-year experience from a Brazilian referral service
AbstractThe use of photobiomodulation therapy (PBMT) in the prevention of oral mucositis (OM) in paediatric care has increased. In this article, we report data of paediatric oncology/haematopoietic stem cell transplantation (HSCT) patients treated with PBMT to prevent chemotherapy-induced OM. A retrospective study was conducted at a Brazilian referral service. Prophylactic PBMT was used in children and adolescents ( ≤ 17 years) following the protocol: InGaAIP, 660 nm, 100 mW, 2 J, 3.33 W/cm2, and 20 s per point. Demographic data and OM severity scores were assessed. A regression model tested the association between OM w...
Source: Lasers in Medical Science - September 22, 2020 Category: Laser Surgery Source Type: research

The efficacy of selective retina therapy for diabetic macular edema based on pretreatment central foveal thickness
AbstractTo evaluate the efficacy of selective retina therapy (SRT) in patients with diabetic macular edema (DME) based on pretreatment central foveal thickness (CFT). Seventy-two eyes of 63 patients with DME who had previously undergone SRT were included. Patients were divided into two groups based on the CFT at baseline. Group 1 was composed of 35 eyes with CFT  
Source: Lasers in Medical Science - September 22, 2020 Category: Laser Surgery Source Type: research

42  °C heat stress pretreatment protects human melanocytes against 308-nm laser-induced DNA damage in vitro
In this study, we combined heat stress pretreatment with UVB to evaluate whether heat stress pretreatment has an ameliorative effect on UVB-induced damage. Human primary melanocytes (HMCs) were cultured and irradiated with a 308-nm laser with/without heat treatment. MTT assay, apoptosis analysis, and comet assay were conducted to monitor the damage of HMCs. Western blot and immunofluorescence staining were performed to assess the expression and subcellular localization of HSP70. HMCs heated at 42  °C for 1 h exhibit no cytotoxicity. Furthermore, preheat treatment attenuated the UVB laser-induced injury, reduc...
Source: Lasers in Medical Science - September 22, 2020 Category: Laser Surgery Source Type: research

COVID-19 in dermatology practice: getting back on track
(Source: Lasers in Medical Science)
Source: Lasers in Medical Science - September 22, 2020 Category: Laser Surgery Source Type: research

Stokes polarimetry-based second harmonic generation microscopy for collagen and skeletal muscle fiber characterization
AbstractThe complete polarization state of second harmonic (SH) light was measured and characterized by collagen type I and skeletal muscle fiber using a Stokes vector-based SHG microscope. The polarization states of the SH signal are analyzed in a pixel-by-pixel manner and displayed through two dimensional (2D) Stokes vector images. Various polarization parameters are reconstructed using Stokes values to quantify the polarization properties of SH light. Also, the measurements are extended for different input polarization states to investigate the molecular structure of second harmonic generation (SHG) active molecules suc...
Source: Lasers in Medical Science - September 17, 2020 Category: Laser Surgery Source Type: research

Non-excisional laser therapies for hemorrhoidal disease: a systematic review of the literature
AbstractNon-excisional laser therapies are emerging treatment for grades II and III hemorrhoidal disease (HD). However, so far, their efficiency is based on low-level evidence. Therefore, we aimed to systematically review the efficiency of non-excisional laser therapies for HD. MEDLINE/Pubmed, Web of science, Embase, and Cochrane were searched from database implementation until the April 17th, 2020. We included studies reporting at least one of surgical indicators of postoperative outcomes of laser therapies, encompassing laser hemorrhoidoplasty (LH) and hemorrhoidal laser procedure (HeLP). Fourteen studies describing LH a...
Source: Lasers in Medical Science - September 9, 2020 Category: Laser Surgery Source Type: research

Microbicidal effect of deep ultraviolet light-emitting diode irradiation
(Source: Lasers in Medical Science)
Source: Lasers in Medical Science - September 8, 2020 Category: Laser Surgery Source Type: research

Evaluation of photobiomodulation therapy to accelerate bone formation in the mid palatal suture after rapid palatal expansion: a randomized clinical trial
AbstractTo evaluate the efficiency of photobiomodulation therapy (PBMT) in the midpalatal suture (MPS) and pain sensation in patients undergoing rapid palatal expansion (RPE). Thirty-four individuals with the diagnosis of skeletal maxillary hypoplasia were divided in two groups: laser (n = 18) and control (n = 16). Treatment plan consisted of the use of the Hyrax expander in all patients. Subjects in the laser group were irradiated with diode laser (980 nm, 0.3 W) in six spots bilaterally distributed along the MPS for 10 s during the active phase of treatment and after overcorrect...
Source: Lasers in Medical Science - September 7, 2020 Category: Laser Surgery Source Type: research

Multimodal imaging with integrated auto-fluorescence and optical coherence tomography for identification of neck tissues
We report a multimodal optical system by combining OCT with autofluorescence imaging for identifying neck tissues, which can use the advantages of large field of view and high sensitivity for identifying parathyroid glands of fluorescence imaging, and high-resolution structural imaging of OCT to confirm them and identify lymph nodes and metastatic lymph nodes at the same time. It is proven that this multimodal optical system can be used to identify different neck tissues effectively and efficiently. We think that integrated auto-fluorescence and OCT imaging have the great potential in the application of navigation and assi...
Source: Lasers in Medical Science - September 7, 2020 Category: Laser Surgery Source Type: research

Nonsurgical periodontal therapy with/without 980  nm diode laser in patients after myocardial infarction: a randomized clinical trial
AbstractThe purpose of this study was to evaluate the possible benefits (in terms of periodontal status improvement and periodontal bacteria count reduction) of using 980  nm diode laser in the treatment of periodontitis in patients after myocardial infarction. Thirty-six patients under 65 years of age (mean: 56.3 ± 7.9) with periodontitis, 6 weeks to 6 months after myocardial infarction, were recruited for the study. The control group (n = 18) received SRP (scaling, root planing and polishing) while the test group (n = 18) received SRP followed by laser ...
Source: Lasers in Medical Science - September 3, 2020 Category: Laser Surgery Source Type: research

Role of LASERS in stage 4A retinopathy of prematurity (ROP)
AbstractTo study the anatomical success rate of light amplification by stimulated emission of radiation (LASERS) as first line of management in stage 4A retinopathy of prematurity (ROP). Observational, prospective case series of 14 eyes of 7 babies (males: 3, females: 4) with stage 4A ROP who underwent LASERS for stage 4A between January 2018 and July 2019. Gestation age (GA), birth weight (BW), and post-menstrual age (PMA) at which laser was done were noted in all cases. A number of clock hours of detachment at the time of presentation were noted in all babies. All babies were followed up up to 6 months after laser for an...
Source: Lasers in Medical Science - September 2, 2020 Category: Laser Surgery Source Type: research

Effect of photobiomodulation therapy on radiodermatitis in a mouse model: an experimental animal study
This study aimed to evaluate the effect of photobiomodulation (PBM) for prevention of radiodermatitis in an irradiated mouse model and compare the efficacy of PBM using 633- or 830-nm wavelengths. Irradiated mice were randomly distributed into three groups: A (633  nm), B (830 nm), and C (without PBM). On post-irradiation days 7 and 21, we compared acute damage and recovery in treated skin samples to non-irradiated skin using H&E, Masson ’s trichrome, anti-CD45 and PCNA immunohistochemistry, and a TUNEL assay. Grade 3 radiodermatitis was evident only in group C. Compared with that in group C, the skin i...
Source: Lasers in Medical Science - September 1, 2020 Category: Laser Surgery Source Type: research

miR-27b promotes angiogenesis and skin repair in scalded rats through regulating VEGF-C expression
In this study, the effects of miR-27b on angiogenesis in skin repair procedure in rats with deep II degree scald were explored. The rat model of deep II scald was established. miR-27b mimics and inhibitor were injected daily at the wound site for 3  weeks. The healing of scald was observed at 0, 3, 7, 14, and 21 days after the model was established, and the pathological changes of skin were observed by HE and Masson’s trichrome stains. Skin tissues were taken 14 days after the operation; CD31 and Ki-67 immunohistochemistry was exerted to evaluate neovascularization and proliferation. Human microvascula...
Source: Lasers in Medical Science - August 13, 2020 Category: Laser Surgery Source Type: research

Attenuation of the inflammatory response and polarization of macrophages by photobiomodulation
AbstractIn spinal cord injury (SCI), inflammation is a major mediator of damage and loss of function and is regulated primarily by the bone marrow-derived macrophages (BMDMs). Photobiomodulation (PBM) or low-level light stimulation is known to have anti-inflammatory effects and has previously been used in the treatment of SCI, although its precise cellular mechanisms remain unclear. In the present study, the effect of PBM at 810  nm on classically activated BMDMs was evaluated to investigate the mechanisms underlying its anti-inflammatory effects. BMDMs were cultured and irradiated (810 nm, 2 mW/cm2) followi...
Source: Lasers in Medical Science - August 13, 2020 Category: Laser Surgery Source Type: research

Experimental investigations on thermal effects of a long-pulse alexandrite laser on blood vessels and its comparison with pulsed dye and Nd:YAG lasers
In this study, the dorsal chamber model and microscopic visualization system were used to observe morphology changes on 42 bloo d vessels before and after irradiation with the 755 nm laser. Results showed that thermal effects of blood vessels intensified with the increase in energy, and high energy was required to produce the same thermal effect as the extension of pulse width. Different from 595 and 1064 nm lasers, partia l vessel contraction was dominant thermal effect caused by the 755 nm laser. The bleeding injury rate and thermal effect of the 755 nm laser were between those of 595 nm PDL and ...
Source: Lasers in Medical Science - August 13, 2020 Category: Laser Surgery Source Type: research

Effect of the usage of Er,Cr:YSGG laser with and without different remineralization agents on the enamel erosion of primary teeth
AbstractThe aim of the present study was to evaluate the effects of different remineralization agents associated with erbium, chromium:yttrium-scandium-gallium-garnet (Er,Cr:YSGG) (0.5  W power, 20 Hz frequency, 60% water, 40% air, 25 mJ pulse energy, 8.84 J/cm2 fluence, 60  μs pulse duration, 600 μm tip diameter, and an approximate 1–1.5 mm distance to the target) laser irradiation on erosion induced by the consumption of carbonated drinks in human primary enamel. There were 8 groups and 10 primary teeth in each g0roup. The distribution was as follows: group 1, casein phospho...
Source: Lasers in Medical Science - August 13, 2020 Category: Laser Surgery Source Type: research

Evaluation of the clinical and biochemical efficacy of erbium, chromium:ytrium-scandium-gallium-garnet (ER,CR:YSGG) laser treatment in periodontitis
The objective of this study was to evaluate the clinical and biochemical efficacy of erbium, chromium:ytrium-scandium-gallium-garnet (Er,Cr:YSGG) laser treatment, in addition to non-surgical periodontal treatment (NSPT), in periodontitis patients. This was a single-centre, split-mouth, randomized, controlled 6-month clinical trial. Twenty-seven patients with moderate to advanced periodontitis were included in the study. The patients were randomly assigned to a debridement and laser treatment group or control group (debridement alone). Clinical measurements were performed at baseline and 1, 3 and 6  months post-treatme...
Source: Lasers in Medical Science - August 13, 2020 Category: Laser Surgery Source Type: research

Treatment of henna-induced Riehl ’s melanosis with a 755-nm picosecond alexandrite laser
(Source: Lasers in Medical Science)
Source: Lasers in Medical Science - August 13, 2020 Category: Laser Surgery Source Type: research

Deep sedation for laser treatment of cutaneous vascular anomalies in children
This report describes our institutional experience with deep sedation in children with capillary vascular malformation, undergoing laser pulse treatment. (Source: Lasers in Medical Science)
Source: Lasers in Medical Science - August 13, 2020 Category: Laser Surgery Source Type: research

Biochemical changes in Leishmania braziliensis after photodynamic therapy with methylene blue assessed by the Fourier transform infrared spectroscopy
AbstractPhotodynamic therapy (PDT) with photosensitizer methylene blue was applied toLeishmania braziliensis, and Fourier transform infrared (FTIR) spectroscopy was used to study biochemical changes in the parasite after PDT in comparison to untreated (C), only irradiation (I), and only photosensitizer (PS). Spectral analysis suggests increase in lipids, proteins, and protein secondary structures in PDT compared with C and decrease in nucleic acids and carbohydrates. Interestingly, these trends are different from PDT ofLeishmania major species, wherein lipids decrease; there are minimal changes in secondary structures and ...
Source: Lasers in Medical Science - August 2, 2020 Category: Laser Surgery Source Type: research

Clinical outcomes of double continuous suture in femtosecond laser-assisted lamellar keratoplasty for keratoconus
AbstractTo assess the surgical outcomes in patients who underwent femtosecond laser-assisted lamellar keratoplasty with double continuous suture for keratoconus, 100 patients (102 eyes) with keratoconus in advanced stages undergoing femtosecond laser-assisted lamellar keratoplasty in Shandong Eye Hospital were studied. In the management of keratoconus, 50 patients (52 eyes) received double continuous suture, and 50 patients (50 eyes) underwent interrupted suture. The follow-up duration was 1  year. Best-corrected visual acuity (BCVA), corneal astigmatism, cosmetic outcomes, and surgical complications were measured as ...
Source: Lasers in Medical Science - August 2, 2020 Category: Laser Surgery Source Type: research

Long-pulsed 1064-nm and 755-nm lasers for C1 leg veins on skin type IV patients: a side-by-side comparison
AbstractLong-pulsed 1064-nm (LP1064) and 755-nm (LP755) lasers have been demonstrated as effective treatments for leg veins. However, few studies of these treatments on Asian skin type as well as direct comparison between two methods were reported. The aim of this study was to compare the clinical efficacy and safety of LP1064 with LP755 in the treatment of C1 leg veins on skin type IV patients. Patients with symmetric matched areas C1 leg veins were treated with single session of LP1064 for the right and LP755 for the left. Treated areas of every patient were divided into matrices of 2  × 2 cm squ...
Source: Lasers in Medical Science - August 2, 2020 Category: Laser Surgery Source Type: research

Effect of diode laser application as an adjunct to nonsurgical periodontal therapy on the reduction of red complex microorganisms in type 2 diabetics with chronic periodontitis
The objective of this study was investigating the additional effect of DL with nonsurgical periodontal treatment on the red complex bacteria in type 2 diabetes mellitus (DM) patients with chronic periodontitis (CP). Sixty type 2 DM patients with chronic periodontitis (CP) were randomly assigned in two parallel groups to receive scaling root planning (SRP,n = 30) or SRP followed by DL periodontal pocket irradiation (SRP + DL,n = 30). Recording of clinical parameters and subgingival plaque sampling were performed at baseline, and post therapy (1 and 3 months after treatment). Amounts ofPorphy...
Source: Lasers in Medical Science - July 10, 2020 Category: Laser Surgery Source Type: research

The effect of electric field, magnetic field, and infrared ray combination to reduce HOMA-IR index and GLUT 4 in diabetic model of Mus musculus
This study used 30 adult male mice strain BALB/c. Diabetes was induced using high-fat diet/streptozotocin method until random blood glucose level reached> 200 mg/dL. Diabetic mice were then exposed to electrical field (static and dynamic), magnetic field (static and induce), and infrared ray (with or without infrared ray) combination therapy 15 min daily for 28 days. Fasting blood glucose level, plasma insulin level, HOMA-IR index, and membrane GLUT-4 density after treatment were analyzed statistically atα = 0.05. Result showed that exposure combination of electrical field, magnetic field, and infrared were found ...
Source: Lasers in Medical Science - July 10, 2020 Category: Laser Surgery Source Type: research

Low-level laser therapy and narrative review of other treatment modalities in androgenetic alopecia
AbstractAndrogenetic alopecia (AGA), also termed as androgenic alopecia or common baldness, is a condition where there is androgen mediated conversion of susceptible terminal hair into vellus hair. Although it is reported more commonly in males, it also affects females but the incidence is relatively unknown. AGA tremendously affects the psychology of the patient due to its chronicity of treatment and cosmetic implications. There are numerous treatment options available for AGA but the choice of treatment has to often be tailored according to the patient ’s needs, affordability, and compliance. This review focusses o...
Source: Lasers in Medical Science - July 10, 2020 Category: Laser Surgery Source Type: research