Green Light photoselective vaporization of the prostate: a safe and effective treatment for elderly high-risk benign prostate hyperplasia patients with gland over 80  ml
AbstractTo analyze the efficacy of Green Light photoselective vaporization of the prostate (PVP) in elderly high-risk benign prostatic hyperplasia (BPH) patients with glands over 80  ml. From December 2013 to February 2016, we allocated 84 elderly (age 71–97) high-risk patients who underwent preoperative transrectal ultrasound (TRUS) examination with glands over 80 ml and divided them into two groups to receive 120 W (n = 40) and 180 W (n = 44) PVP. All the patients have been observed at least one intraoperative comorbidity: hypertension, diabetes mellitus, NYHA II, or ...
Source: Lasers in Medical Science - October 16, 2018 Category: Laser Surgery Source Type: research

Resistance to vertical root fracture of apicoected teeth using different devices during two root canal irrigation procedures
AbstractThe aim of the present work was to measure the fracture resistance of endodontically treated teeth that were apicoected with different procedures. Seventy-two extracted human maxillary anterior teeth were included in this study. The specimens were randomly assigned to three main groups according to the apical surgery procedures and then two subgroups according to the irrigation protocols during root canal treatment and total of six groups were obtained (n = 12). Group 1: served as a control and apical surgery process was not performed in this group. Group 2: apical surgery process was performed with t...
Source: Lasers in Medical Science - October 16, 2018 Category: Laser Surgery Source Type: research

Appropriate laser wavelengths for photodynamic therapy with methylene blue
(Source: Lasers in Medical Science)
Source: Lasers in Medical Science - October 16, 2018 Category: Laser Surgery Source Type: research

Effects of low-level laser therapy on soft and hard tissue healing after endodontic surgery
This study concluded that LLLT improved soft and hard tissue healing after endodontic surgery and also showed favorable effects on pain and life quality of patients especially in the early phase of healing period. (Source: Lasers in Medical Science)
Source: Lasers in Medical Science - October 16, 2018 Category: Laser Surgery Source Type: research

Correction to: Comparative study using fractional carbon dioxide laser versus glycolic acid peel in treatment of pseudo-acanthosis nigricans
The author found small mistakes in scientific content of article. Editor-in-Chief confirmed that mistakes do not change or invalidate conclusions of article. (Source: Lasers in Medical Science)
Source: Lasers in Medical Science - October 16, 2018 Category: Laser Surgery Source Type: research

Comparison of diagnostic effects of infrared imaging and bitewing radiography in proximal caries of permanent teeth
This study aimed to compare the diagnostic efficacy of VistaCam iX intraoral camera system using infrared light and bitewing radiography for detection of proximal caries in permanent teeth. This in vitro study was performed on 108 teeth. The proximal surfaces of the teeth were examined for caries using ICDAS II criteria, bitewing radiography, and the Proxi head of VistaCam iX. The teeth were then sectioned and histologically analyzed (gold standard). Data were analyzed using SPSS version 25 via the correlation test atP 
Source: Lasers in Medical Science - October 15, 2018 Category: Laser Surgery Source Type: research

Effect of light-emitting diodes, platelet-rich plasma, and their combination on the activity of sheep tenocytes
This study utilizes cell culture to explore the potential effect of light-emitting diode and/or growth factors in the form of platelet-rich plasma (PRP) on the activity of tenocytes isolated from sheep Achilles tendons by measuring the cell metabolism and cell mobility using cell viability and migration assays to proof safety and confirm activity. Results showed that sheep tenocyte-cultured groups treated with 5% platelet-rich plasma alone or combined with 4  J/cm2 light-emitting diode have increased viability significantly when compared to control group after a 48  h, while light-emitting diode treatment has not...
Source: Lasers in Medical Science - October 13, 2018 Category: Laser Surgery Source Type: research

Types of advanced optical microscopy techniques for breast cancer research: a review
AbstractA cancerous cell is characterized by morphological and metabolic changes which are the key features of carcinogenesis. Adenosine triphosphate (ATP) in cancer cells is primarily produced by aerobic glycolysis rather than oxidative phosphorylation. In normal cellular metabolism, nicotinamide adenine dinucleotide (NADH) is considered as a principle electron donor and flavin adenine dinucleotide (FAD) as an electron acceptor. During metabolism in a cancerous cell, a net increase in NADH is found as the pathway switched from oxidative phosphorylation to aerobic glycolysis. Often during initiation and progression of canc...
Source: Lasers in Medical Science - October 11, 2018 Category: Laser Surgery Source Type: research

Multi-wavelength laser treatments of spider nevi
AbstractSpider nevi (SN) are one of common vascular diseases. Different treatment techniques have been described for SN previously, including electrocoagulation, argon laser, pulsed dye lasers (PDL), pulsed potassium titanylphosphate laser (KTP), and 1064-nm neodymium yttrium-aluminum-garnet (Nd:YAG) laser. These methods are effective but may require good technical management, multiple treatments, and often result in scarring or pigmentation. Multi-wavelength laser combined with 595-nm PDL followed by 1064-nm Nd:YAG and can be selectively absorbed by hemoglobin in vessels. The 595-nm laser can target shallow vessels wherea...
Source: Lasers in Medical Science - October 11, 2018 Category: Laser Surgery Source Type: research

Spectroscopic study of breath ethylene via the mouth and nose
AbstractThe development of new techniques for breath analysis searching for objective biomarkers of oxidative stress showed promise in non-invasive disclosing health information of the well-being of a person. Although numerous biomarkers have been identified so far using breath analysis, very little is known about their origin if they are metabolic or providing from mouth contamination. For the introduction of breath tests into clinical practice, standardization of sample collection needs to be taken into account. Breath analysis has been performed using laser photoacoustic spectroscopy to evaluate exhaled breath by mouth ...
Source: Lasers in Medical Science - October 11, 2018 Category: Laser Surgery Source Type: research

Efficacy of photobiomodulation on accelerating bone healing after tooth extraction: a systematic review
AbstractPost-extraction healing of the socket may take up to 24  weeks to complete. This systematic review aims to evaluate whether photobiomodulation accelerates bone healing in those sockets. A search strategy was developed in line with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement. PubMed, Cochrane Library, and Sco pus electronic databases were searched for in vivo studies with restrictions on the year (
Source: Lasers in Medical Science - October 11, 2018 Category: Laser Surgery Source Type: research

Effects of antibacterial photodynamic therapy on salivary mutans streptococci in 5- to 6-year-olds with severe early childhood caries
This study sought to assess the antimicrobial effect of A-PDT with toluidine blue O (TBO) and diode laser on salivary mutans streptococci in 5 –6-year-olds with severe early childhood caries (SECC). This case-control study was conducted on 56 children with SECC divided into four groups, namely 0.1 mg/mL TBO, diode laser (633 nm, 20 mW, 6 J/cm2), combination of the two, and no intervention control group. A-PDT was performed on days 1 and 3. Salivary samples were collected before and after A-PDT on days 1 and 3, and 1 and 2  weeks after the second intervention (day 3). Samples were cultured on ...
Source: Lasers in Medical Science - October 11, 2018 Category: Laser Surgery Source Type: research

Melanocyte activation and skin barrier disruption induced in melasma patients after 1064  nm Nd:YAG laser treatment
AbstractMelasma is a frequently acquired hyperpigmentary skin disorder, for which several therapies are available. Among them, 1064  nm QS Nd:YAG laser therapy is an effective method, but the recurrence rate of laser treatment is still high. The aim of the present study was to elucidate the mechanism of the high relapse rate of melasma after 1064 nm Nd:YAG laser treatment. Twenty-five female melasma patients were treated with 1064 nm Nd:YAG laser for 10 times. The lesional skin and non-lesional skin were evaluated by means of a reflectance confocal laser scanning microscope before and after laser treatment. ...
Source: Lasers in Medical Science - October 9, 2018 Category: Laser Surgery Source Type: research

Letter about: nocturnal enuresis in children between laser acupuncture and medical treatment —a comparative study
(Source: Lasers in Medical Science)
Source: Lasers in Medical Science - October 8, 2018 Category: Laser Surgery Source Type: research

Low-power laser alters mRNA levels from DNA repair genes in acute lung injury induced by sepsis in Wistar rats
AbstractAcute lung injury (ALI) is defined as respiratory failure syndrome, in which the pathogenesis could occur from sepsis making it a life-threatening disease by uncontrolled hyperinflammatory responses. A possible treatment for ALI is the use of low-power infrared lasers (LPIL), whose therapeutical effects depend on wavelength, power, fluence, and emission mode. The evaluation mRNA levels of repair gene related to oxidative damage after exposure to LPIL could provide important information about the modulation of genes as treatment for ALI. Thus, the aim of this study was to evaluate the mRNA levels fromOGG1,APEX1,ERCC...
Source: Lasers in Medical Science - October 8, 2018 Category: Laser Surgery Source Type: research

Effectiveness of photopolymerization in composite resins using a novel 445-nm diode laser in comparison to LED and halogen bulb technology
AbstractChallenges especially in the minimal invasive restorative treatment of teeth require further developments of composite polymerization techniques. These include, among others, the securing of a complete polymerization with moderate thermal stress for the pulp. The aim of this study is to compare current light curing sources with a blue diode laser regarding curing depth and heat generation during the polymerization process. A diode laser (445  nm), a LED, and a halogen lamp were used for polymerizing composite resins. The curing depth was determined according to the norm ISO 4049. Laser output powers of 0.1, 0....
Source: Lasers in Medical Science - October 6, 2018 Category: Laser Surgery Source Type: research

Photobiomodulation on critical bone defects of rat calvaria: a systematic review
AbstractBone defects following trauma represent a high impact on the quality of life of millions of people around the world. The aim of this study was to review photobiomodulation (PBM) action in the treatment of bone critical defects in rat calvaria, related to evaluation of the current protocols applied. One hundred and forty-seven articles related to the subject were found by searching the main databases (Pubmed, Lilacs, Web of Science, and Scopus) considering the period of publication until the year 2017, and only 14 corresponded the inclusion criteria established for this systematic review. The main parameters of the ...
Source: Lasers in Medical Science - October 6, 2018 Category: Laser Surgery Source Type: research

Antifungal efficacy of photodynamic therapy with TONS 504 for pathogenic filamentous fungi
AbstractThe pathogenic filamentous fungiFusarium solani (F. solani) andAspergillus fumigatus (A. fumigatus) are common causes of fungal keratitis. We have here evaluated the antifungal efficacy of photodynamic antimicrobial chemotherapy (PACT) with the novel chlorin derivative TONS 504 and a light-emitting diode (LED) with a wavelength of 660  nm for these fungal species. Isolated fungal spores were irradiated at LED energies of 10, 20, or 30 J/cm2 in the presence of TONS 504 at concentrations of 1 or 10  mg/L. As a control, spores were exposed to TONS 504 or LED radiation alone. The treated spores were then...
Source: Lasers in Medical Science - October 4, 2018 Category: Laser Surgery Source Type: research

Photobiomodulation effect on the proliferation of adipose tissue mesenchymal stem cells
This study aimed to evaluate the PBM action with different energies in MSCs of adipose tissue (hASCs). We used hASCs, seeded in 24-well plates, with 3  × 104 cells per well, in culture media. We used a total of four experimental groups, one with hASCs and simulated PBM and three other groups, which received PBM irradiation at 24, 48, and 72  h, with a 660-nm laser and power of 40 mW and energy of 0.56, 1.96, and 5.04 J. We performed analyses of MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromidefor) and trypan blue to evaluate cell proliferation and viability, 1 h after...
Source: Lasers in Medical Science - October 3, 2018 Category: Laser Surgery Source Type: research

Quantitative evaluation of skin shrinkage associated with non-invasive skin tightening: a simple method for reproducible linear measurement using microtattoos
AbstractNon-invasive skin-tightening devices can induce thermal denaturation and skin shrinkage via externally applied radiofrequency emissions or high-frequency ultrasound. Therefore, the purpose of this study is to develop and test a method for measurement of skin reduction associated with application of such energy devices. Twenty-five healthy participants with mild to moderate skin laxity of the arms were enrolled. Pinpoint microtattoos were placed at each of the treatment sites to delineate two 6  × 12 cm rectangles per subject. A non-stretchable filament, tape and marking pen apparatus was us...
Source: Lasers in Medical Science - October 3, 2018 Category: Laser Surgery Source Type: research

Photobiomodulation effect on the proliferation of adipose tissue mesenchymal stem cells
This study aimed to evaluate the PBM action with different energies in MSCs of adipose tissue (hASCs). We used hASCs, seeded in 24-well plates, with 3  × 104 cells per well, in culture media. We used a total of four experimental groups, one with hASCs and simulated PBM and three other groups, which received PBM irradiation at 24, 48, and 72  h, with a 660-nm laser and power of 40 mW and energy of 0.56, 1.96, and 5.04 J. We performed analyses of MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromidefor) and trypan blue to evaluate cell proliferation and viability, 1 h after...
Source: Lasers in Medical Science - October 3, 2018 Category: Laser Surgery Source Type: research

Quantitative evaluation of skin shrinkage associated with non-invasive skin tightening: a simple method for reproducible linear measurement using microtattoos
AbstractNon-invasive skin-tightening devices can induce thermal denaturation and skin shrinkage via externally applied radiofrequency emissions or high-frequency ultrasound. Therefore, the purpose of this study is to develop and test a method for measurement of skin reduction associated with application of such energy devices. Twenty-five healthy participants with mild to moderate skin laxity of the arms were enrolled. Pinpoint microtattoos were placed at each of the treatment sites to delineate two 6  × 12 cm rectangles per subject. A non-stretchable filament, tape and marking pen apparatus was us...
Source: Lasers in Medical Science - October 3, 2018 Category: Laser Surgery Source Type: research

Prediction of femtosecond laser ablation profile on human teeth
AbstractTo predict the laser ablation profile on dental hard tissue which will enable the user to optimize laser parameters so as to carry out the laser treatment with minimal tissue damage. The present study constructs a mathematical model to predict the ablation profile based on Gaussian beam distribution of laser intensity and correlates the model with experimentally obtained ablation parameters (effective Gaussian beam radius, ablation threshold fluence, and effective energy penetration depth). To obtain the ablation parameters, laser ablation experiments were carried out on dental hard tissues using Ti:Sapphire femtos...
Source: Lasers in Medical Science - October 2, 2018 Category: Laser Surgery Source Type: research

Effect of blue LED on the healing process of third-degree skin burns: clinical and histological evaluation
AbstractThe aim of this study was to evaluate the effects of blue light-emitting diode (LED) on the healing process of third-degree skin burns in rats through clinical and histological parameters. Forty male Wistar rats were divided into two groups: control (CTR) (n = 20) and blue LED (BLUE) (n = 20), with subgroups (n = 5) for each time of euthanasia (7, 14, 21, and 28 days). LED (470 nm, 1 W, 12.5 J/cm2 per point, 28  s) was applied at four points of the wound (total, 50 J/cm2). Feed intake was measured every other day. It was observed that there wer...
Source: Lasers in Medical Science - October 1, 2018 Category: Laser Surgery Source Type: research

Q-switched 1064  nm Nd-Yag nanosecond laser effects on skin barrier function and on molecular rejuvenation markers in keratinocyte-fibroblasts interaction
The objective of this study was to evaluate the effect of Q-switched 1064 Nd-YAG laser (Medlite Conbio C6 Nd-YAG laser, Cynosure USA) in skin barrier function, analyzing the constituents which are strongly altered in aging skin. Particularly, we evaluated the expression of filaggrin, TGase, HSP70, and aquaporins, on HaCaT cells. The expression of proinflammatory cytokines has been investigated too.As a second step of the study, we analyzed the modulation of the rejuvenation molecular markers on human skin fibroblasts (HDFs) stimulated with keratinocytes conditioned medium (KCM).Our results demonstrated that Q-switched 1064...
Source: Lasers in Medical Science - October 1, 2018 Category: Laser Surgery Source Type: research

Effect of blue LED on the healing process of third-degree skin burns: clinical and histological evaluation
AbstractThe aim of this study was to evaluate the effects of blue light-emitting diode (LED) on the healing process of third-degree skin burns in rats through clinical and histological parameters. Forty male Wistar rats were divided into two groups: control (CTR) (n = 20) and blue LED (BLUE) (n = 20), with subgroups (n = 5) for each time of euthanasia (7, 14, 21, and 28 days). LED (470 nm, 1 W, 12.5 J/cm2 per point, 28  s) was applied at four points of the wound (total, 50 J/cm2). Feed intake was measured every other day. It was observed that there wer...
Source: Lasers in Medical Science - October 1, 2018 Category: Laser Surgery Source Type: research

Q-switched 1064  nm Nd-Yag nanosecond laser effects on skin barrier function and on molecular rejuvenation markers in keratinocyte-fibroblasts interaction
The objective of this study was to evaluate the effect of Q-switched 1064 Nd-YAG laser (Medlite Conbio C6 Nd-YAG laser, Cynosure USA) in skin barrier function, analyzing the constituents which are strongly altered in aging skin. Particularly, we evaluated the expression of filaggrin, TGase, HSP70, and aquaporins, on HaCaT cells. The expression of proinflammatory cytokines has been investigated too.As a second step of the study, we analyzed the modulation of the rejuvenation molecular markers on human skin fibroblasts (HDFs) stimulated with keratinocytes conditioned medium (KCM).Our results demonstrated that Q-switched 1064...
Source: Lasers in Medical Science - October 1, 2018 Category: Laser Surgery Source Type: research

Photobiomodulation therapy before futsal matches improves the staying time of athletes in the court and accelerates post-exercise recovery
This study aimed  to analyze PBMT effects on futsal player’s performance and recovery in a non-controlled field environment. It is a randomized, triple-blinded, placebo-controlled, crossover clinical trial. The research included six professional athletes and in each match phototherapy treatments were performed be fore matches (40 minutes), blood samples were collected before treatments, and samples immediately after the end of the matches and 48 h after. Furthermore, videos were analyzed to quantify the time athletes spent on the pitch and the distance they covered. PBMT was performed at 17 sites of ea...
Source: Lasers in Medical Science - September 27, 2018 Category: Laser Surgery Source Type: research

Photobiomodulation with single and combination laser wavelengths on bone marrow mesenchymal stem cells: proliferation and differentiation to bone or cartilage
AbstractTissue engineering aims to take advantage of the ability of undifferentiated stem cells to differentiate into multiple cell types to repair damaged tissue. Photobiomodulation uses either lasers or light-emitting diodes to promote stem cell proliferation and differentiation. The present study aimed to investigate single and dual combinations of laser wavelengths on mesenchymal stem cells (MSCs). MSCs were derived from rabbit iliac bone marrow. One control and eight laser irradiated groups were designated as Infrared (IR, 810  nm), Red (R, 660 nm), Green (G, 532 nm), Blue (B, 485 nm), IR–R, ...
Source: Lasers in Medical Science - September 27, 2018 Category: Laser Surgery Source Type: research

Physicochemical, morphological, and biological analyses of Ti-15Mo alloy surface modified by laser beam irradiation
AbstractPerform a physicochemical and morphological characterization of a Ti-15Mo alloy surface modified by laser beam irradiation and to evaluate in vitro the morphological response and proliferation of osteoblastic cells seeded onto this alloy. Disks were made of two different metals, Ti-15Mo alloy and cpTi, used as control. A total of four groups were evaluated: polished cpTi (cpTi-pol), laser-irradiated cpTi (cpTi-L), polished Ti-15Mo alloy (Ti-15Mo-pol), and laser-irradiated Ti-15Mo alloy (Ti-15Mo-L). Before and after laser irradiation of the surfaces, physicochemical and morphological analyses were performed: scannin...
Source: Lasers in Medical Science - September 27, 2018 Category: Laser Surgery Source Type: research

Surface electromyography after lower level laser therapy application on skeletal muscles in individuals with heart failure
AbstractThe purpose of this study was to investigate the effects of low-level laser therapy (LLLT) applied before a fatigue protocol through the effects on the electrical activation in the quadriceps muscle in patients with HF. Fourteen patients with the diagnosis of heart failure (HF) were selected for this double-blind, crossover type clinical trial. These participants have attended to a familiarization, LLLT, and placebo sessions, totaling three visits. The LLLT was applied in the quadriceps muscle (850  nm, 5 J per diode). The fatigue protocol consisted of concentric and eccentric isokinetic contractions (cc/...
Source: Lasers in Medical Science - September 27, 2018 Category: Laser Surgery Source Type: research

Photobiomodulation therapy before futsal matches improves the staying time of athletes in the court and accelerates post-exercise recovery
This study aimed  to analyze PBMT effects on futsal player’s performance and recovery in a non-controlled field environment. It is a randomized, triple-blinded, placebo-controlled, crossover clinical trial. The research included six professional athletes and in each match phototherapy treatments were performed be fore matches (40 minutes), blood samples were collected before treatments, and samples immediately after the end of the matches and 48 h after. Furthermore, videos were analyzed to quantify the time athletes spent on the pitch and the distance they covered. PBMT was performed at 17 sites of ea...
Source: Lasers in Medical Science - September 27, 2018 Category: Laser Surgery Source Type: research

Photobiomodulation with single and combination laser wavelengths on bone marrow mesenchymal stem cells: proliferation and differentiation to bone or cartilage
AbstractTissue engineering aims to take advantage of the ability of undifferentiated stem cells to differentiate into multiple cell types to repair damaged tissue. Photobiomodulation uses either lasers or light-emitting diodes to promote stem cell proliferation and differentiation. The present study aimed to investigate single and dual combinations of laser wavelengths on mesenchymal stem cells (MSCs). MSCs were derived from rabbit iliac bone marrow. One control and eight laser irradiated groups were designated as Infrared (IR, 810  nm), Red (R, 660 nm), Green (G, 532 nm), Blue (B, 485 nm), IR–R, ...
Source: Lasers in Medical Science - September 27, 2018 Category: Laser Surgery Source Type: research

Physicochemical, morphological, and biological analyses of Ti-15Mo alloy surface modified by laser beam irradiation
AbstractPerform a physicochemical and morphological characterization of a Ti-15Mo alloy surface modified by laser beam irradiation and to evaluate in vitro the morphological response and proliferation of osteoblastic cells seeded onto this alloy. Disks were made of two different metals, Ti-15Mo alloy and cpTi, used as control. A total of four groups were evaluated: polished cpTi (cpTi-pol), laser-irradiated cpTi (cpTi-L), polished Ti-15Mo alloy (Ti-15Mo-pol), and laser-irradiated Ti-15Mo alloy (Ti-15Mo-L). Before and after laser irradiation of the surfaces, physicochemical and morphological analyses were performed: scannin...
Source: Lasers in Medical Science - September 27, 2018 Category: Laser Surgery Source Type: research

Surface electromyography after lower level laser therapy application on skeletal muscles in individuals with heart failure
AbstractThe purpose of this study was to investigate the effects of low-level laser therapy (LLLT) applied before a fatigue protocol through the effects on the electrical activation in the quadriceps muscle in patients with HF. Fourteen patients with the diagnosis of heart failure (HF) were selected for this double-blind, crossover type clinical trial. These participants have attended to a familiarization, LLLT, and placebo sessions, totaling three visits. The LLLT was applied in the quadriceps muscle (850  nm, 5 J per diode). The fatigue protocol consisted of concentric and eccentric isokinetic contractions (cc/...
Source: Lasers in Medical Science - September 27, 2018 Category: Laser Surgery Source Type: research

Acute effects of photobiomodulation therapy (PBMT) combining laser diodes, light-emitting diodes, and magnetic field in exercise capacity assessed by 6MST in patients with COPD: a crossover, randomized, and triple-blinded clinical trial
This study showed that the combined application of PBMT and magnetic field increased the number of steps during the 6MST and decreased the sensation of dyspnea and lower limb fatigue in patients with COPD. (Source: Lasers in Medical Science)
Source: Lasers in Medical Science - September 25, 2018 Category: Laser Surgery Source Type: research

Correction to: Glucose in Conjunction with Multiple Laser Pulses on Laser Treatment of Port-wine Stain: An in vivo Study
In the originally published article, the title was incorrectly copied from another article. The correct title is “Glucose in Conjunction with Multiple Laser Pulses on Laser Treatment of Port-wine Stain: Anin vivo Study ”, which is also shown above. (Source: Lasers in Medical Science)
Source: Lasers in Medical Science - September 25, 2018 Category: Laser Surgery Source Type: research

Acute effects of photobiomodulation therapy (PBMT) combining laser diodes, light-emitting diodes, and magnetic field in exercise capacity assessed by 6MST in patients with COPD: a crossover, randomized, and triple-blinded clinical trial
This study showed that the combined application of PBMT and magnetic field increased the number of steps during the 6MST and decreased the sensation of dyspnea and lower limb fatigue in patients with COPD. (Source: Lasers in Medical Science)
Source: Lasers in Medical Science - September 25, 2018 Category: Laser Surgery Source Type: research

Correction to: Glucose in Conjunction with Multiple Laser Pulses on Laser Treatment of Port-wine Stain: An in vivo Study
In the originally published article, the title was incorrectly copied from another article. The correct title is “Glucose in Conjunction with Multiple Laser Pulses on Laser Treatment of Port-wine Stain: Anin vivo Study ”, which is also shown above. (Source: Lasers in Medical Science)
Source: Lasers in Medical Science - September 25, 2018 Category: Laser Surgery Source Type: research

Short-term evaluation of photobiomodulation therapy on the proliferation and undifferentiated status of dental pulp stem cells
AbstractThe aim of this in vitro study was to analyze the effect of photobiomodulation therapy (PBMT) on the proliferation and undifferentiating status of stem cell from human exfoliated deciduous teeth (SHEDs). PBMT was carried out with an aluminum gallium indium phosphide (InGaAlP) diode laser in contact and punctual mode (continuous wave, 660  nm, 20 mW, 0.028 cm2, and average energy densities of 1 (1  s), 3 (4 s), 5 (7 s), 10 (14 s), 15 (21 s), or 20 (28 s) J/cm2 per point). The immunoprofile of the SHEDs was analyzed using flow cytometry. Cell proliferation was assessed by ...
Source: Lasers in Medical Science - September 24, 2018 Category: Laser Surgery Source Type: research

The role of Wnt/ β-catenin signaling in the restoration of induced pluripotent stem cell-derived retinal pigment epithelium after laser photocoagulation
AbstractTo investigate the role ofWnt/ β-catenin signaling pathway in the restoration of induced pluripotent stem cell-derived retinal pigment epithelium (hiPSC-RPE) after laser photocoagulation. After differentiation of RPE cells from hiPSCs, laser photocoagulation was performed. Activation ofWnt/ β-catenin signaling at days 1 and 5 after laser photocoagulation was evaluated by expression of β-catenin. Cell proliferation and alteration in cell-to-cell contact at day 5 after laser photocoagulation with or without Dickkopf-1 (Dkk-1) treatment were studied using ethynyl-2′-deoxyuridine (EdU) assay and zo...
Source: Lasers in Medical Science - September 22, 2018 Category: Laser Surgery Source Type: research

Wound-healing effects of 635-nm low-level laser therapy on primary human vocal fold epithelial cells: an in vitro study
In this study, normal vocal fold tissue was obtained from a 58-year-old male patient who was diagnosed with postcricoid carcinoma without involvement of the vocal folds and underwent total laryngectomy. Primary VFECs were then cultured. Cells were irradiated at a wavelength of 635  nm with fluences of 1, 4, 8, 12, 16, and 20 J/cm2 (50  mW/cm2), which correspond to irradiation times of 20, 80, 160, 240, 320, and 400  s, respectively. Cell viability of VFECs in response to varying doses of LLLT was investigated by the Cell Counting Kit-8 (CCK-8) method. The most effective irradiation dose was selected to ...
Source: Lasers in Medical Science - September 22, 2018 Category: Laser Surgery Source Type: research

The role of Wnt/ β-catenin signaling in the restoration of induced pluripotent stem cell-derived retinal pigment epithelium after laser photocoagulation
AbstractTo investigate the role ofWnt/ β-catenin signaling pathway in the restoration of induced pluripotent stem cell-derived retinal pigment epithelium (hiPSC-RPE) after laser photocoagulation. After differentiation of RPE cells from hiPSCs, laser photocoagulation was performed. Activation ofWnt/ β-catenin signaling at days 1 and 5 after laser photocoagulation was evaluated by expression of β-catenin. Cell proliferation and alteration in cell-to-cell contact at day 5 after laser photocoagulation with or without Dickkopf-1 (Dkk-1) treatment were studied using ethynyl-2′-deoxyuridine (EdU) assay and zo...
Source: Lasers in Medical Science - September 22, 2018 Category: Laser Surgery Source Type: research

Biochemical changes in injured sciatic nerve of rats after low-level laser therapy (660  nm and 808 nm) evaluated by Raman spectroscopy
AbstractThe aim of this study was to identify biochemical changes in sciatic nerve (SN) after crush injury and low-level laser therapy (LLLT) with 660  nm and 808 nm by Raman spectroscopy (RS) analysis. A number of 32 Wistar rats were used, divided into four groups (control 1, control 2, LASER 660 nm, and LASER 808 nm). All animals underwent surgical procedure of the SN and groups control 2, LASER 660 nm, and LASER 808 nm were submitted to S N crush damage (axonotmesis). The LLLT in the groups LASER 660 nm and LASER 808 nm was applied daily for 21 consecutive days (100 mW, 30&nb...
Source: Lasers in Medical Science - September 22, 2018 Category: Laser Surgery Source Type: research

Wound-healing effects of 635-nm low-level laser therapy on primary human vocal fold epithelial cells: an in vitro study
In this study, normal vocal fold tissue was obtained from a 58-year-old male patient who was diagnosed with postcricoid carcinoma without involvement of the vocal folds and underwent total laryngectomy. Primary VFECs were then cultured. Cells were irradiated at a wavelength of 635  nm with fluences of 1, 4, 8, 12, 16, and 20 J/cm2 (50  mW/cm2), which correspond to irradiation times of 20, 80, 160, 240, 320, and 400  s, respectively. Cell viability of VFECs in response to varying doses of LLLT was investigated by the Cell Counting Kit-8 (CCK-8) method. The most effective irradiation dose was selected to ...
Source: Lasers in Medical Science - September 22, 2018 Category: Laser Surgery Source Type: research

Biochemical changes in injured sciatic nerve of rats after low-level laser therapy (660  nm and 808 nm) evaluated by Raman spectroscopy
AbstractThe aim of this study was to identify biochemical changes in sciatic nerve (SN) after crush injury and low-level laser therapy (LLLT) with 660  nm and 808 nm by Raman spectroscopy (RS) analysis. A number of 32 Wistar rats were used, divided into four groups (control 1, control 2, LASER 660 nm, and LASER 808 nm). All animals underwent surgical procedure of the SN and groups control 2, LASER 660 nm, and LASER 808 nm were submitted to S N crush damage (axonotmesis). The LLLT in the groups LASER 660 nm and LASER 808 nm was applied daily for 21 consecutive days (100 mW, 30&nb...
Source: Lasers in Medical Science - September 22, 2018 Category: Laser Surgery Source Type: research

Photobiomodulation is associated with a decrease in cell viability and migration in oral squamous cell carcinoma
In this study, the effect of PBM on the viability, mitochondrial activity, proliferation, apoptosis, and migration of cells derived from oral SCC was evaluated. SCC9 cells were irradiated with laser (660 and 780  nm, using 11 dosimetric parameters) and submitted to mitochondrial and caspase 3 activity tests after 1 and 3 days. Based on the results, cell viability (neutral red assay), proliferation (BrdU assay), and migration (scratch-wound assay) were evaluated using only the dosimetric parameters recomme nded for mucositis. Non-irradiated cells served as the control. The experiments were performed in triplicate....
Source: Lasers in Medical Science - September 19, 2018 Category: Laser Surgery Source Type: research