Er:YAG laser application in caries removal and cavity preparation in children: a meta-analysis
In conclusion, our data indicate that the time required for Er:YAG laser treatment was longer than that for the conventional mechanical method, but there was less pain associated with the Er:YAG laser treatment. There were no significant differences in the complete retention rate, marginal discoloration, and marginal adaptation between the two groups. (Source: Lasers in Medical Science)
Source: Lasers in Medical Science - July 12, 2018 Category: Laser Surgery Source Type: research

Effects of Nd:YAG low-level laser irradiation on cultured human osteoblasts migration and ATP production: in vitro study
AbstractLow-level laser therapy has become one of the fastest growing fields of medicine in recent years. Many in vivo and in vitro studies have shown that laser irradiation activates a range of cellular processes in a variety of cell types and can promote tissue repair. However, few in vitro experiments have evaluated the effects of laser irradiation on cells in real time. The purpose of this study was to examine the effects of neodymium-doped yttrium aluminum garnet (Nd:YAG) laser irradiation on the migration of cultured human osteoblasts. A dedicated 96-well plate was used, and confluent cultures of the human osteoblast...
Source: Lasers in Medical Science - July 12, 2018 Category: Laser Surgery Source Type: research

Evaluation of the therapeutic effect of micro-plasma radio frequency on hypertrophic scars in rabbit ears
AbstractTo evaluate the therapeutic effect of micro-plasma radio frequency on hypertrophic scars in rabbit ears to provide an experimental basis and theoretical foundation for the treatment of hypertrophic scars. Hypertrophic scars were established on the ventral surface of the ears of six New Zealand white rabbits. Left and right ears were randomly divided into two groups: experimental group treated with micro-plasma radio frequency and control group with no treatment. H&E staining and CD34 labeling of microvessels were performed to analyze ear specimens, and immunohistochemical staining was conducted to detect IL-8 a...
Source: Lasers in Medical Science - July 12, 2018 Category: Laser Surgery Source Type: research

Low-level laser therapy (830  nm) on orthodontic pain: blinded randomized clinical trial
The objective of this research was to compare the effect single low-level laser therapy (LLLT) irradiation on pain perception in patients having fixed appliance treatment in the clinic of orthodontics. Sixty-two patients were recruited to participate in this randomized, double-blinded, placebo-controlled study. The patients were assigned to four groups: group I —laser on the right side; group II—placebo on the right side; group III—laser on the left side; group IV—placebo on the left. The laser or placebo was applied before separation, 24 and 48 h after separation of their first permanent molar...
Source: Lasers in Medical Science - July 12, 2018 Category: Laser Surgery Source Type: research

Efficacy of Nd-YAG laser for treatment of pyogenic granuloma on the fingers and toes
AbstractPyogenic granuloma (PG) is a common benign vascular proliferation which often occurs on the head, neck, hands, and feet. Among the various treatment options for PG, surgical excision is the most effective treatment which offers the lowest overall recurrence rates and also provides the exact diagnosis. However, it could have difficulties to do the surgery when lesions are located on the fingers and toes, especially very near to the nails, so laser may be a very good alternative choice. In this article, we evaluated the clinical efficacy and safety of neodymium-yttrium aluminum garnet (Nd:YAG) laser for treatment of ...
Source: Lasers in Medical Science - July 11, 2018 Category: Laser Surgery Source Type: research

PBMT and topical diclofenac as single and combined treatment on skeletal muscle injury in diabetic rats: effects on biochemical and functional aspects
The objective of the present study was to evaluate the effects of NSAIDs and PBMT applied alone or combined on functional and biochemical aspects, in an experimental model of muscle injury through controlled trauma in diabetic rats. Muscle injury was induced by means of a single trauma to the animals ’ anterior tibialis muscle. After 1 h, the rats were treated with PBMT (830 nm; continuous mode, with a power output of 100 mW; 3.57 W/cm2; 3  J; 107.1 J/cm2, 30  s), diclofenac sodium for topical use (1 g), or combination of them. Our results demonstrated that PBMT + diclofenac, a...
Source: Lasers in Medical Science - July 10, 2018 Category: Laser Surgery Source Type: research

Dentin pretreatment with Er:YAG laser and sodium ascorbate to improve the bond strength of glass fiber post
The objective of this study is to evaluate the effect of Er:YAG laser and/or sodium ascorbate (SA) on bond strength, microhardness of dentin, and penetration depth of cement into dentinal tubules. Forty-eight bovine incisor roots were endodontically treated, post spaces were prepared and equally divided into four groups (n = 12): G1—distilled water (control); G2—10% SA (10 min); G3—Er:YAG laser (150 mJ/4 Hz/40 s), and G4—Er:YAG laser + 10% SA. Glass fiber posts were cemented and roots sectioned intoslices. In the first slice, the push-out bond strength (MPa) an...
Source: Lasers in Medical Science - July 9, 2018 Category: Laser Surgery Source Type: research

Qualitative evaluation of hybrid layer formation using Er:YAG laser in QSP mode for tooth cavity preparations
AbstractThe purpose of this study was to evaluate the thickness and qualitative characteristics of the hybrid layer after two cavity preparation methods, using Er:YAG laser in QSP mode and conventional carbide burs. Additionally, two different adhesive techniques were investigated using etch-and-rinse and self-etch adhesive systems. Sixty sound human third molars were used and were randomly divided into four groups (n = 15). In the first two groups, large (4 mm length, 3 mm wide, and 3 mm deep) class I cavities were prepared using Er:YAG laser (2.94 μm) in QSP mode, while in the other t...
Source: Lasers in Medical Science - July 8, 2018 Category: Laser Surgery Source Type: research

Effectiveness of low concentration of sodium hypochlorite activated by Er,Cr:YSGG laser against Enterococcus faecalis biofilm
AbstractBacteria living in biofilms exhibit altered growth phenotypes, while the biofilm provides benefits, the foremost of which is a certain protection against both immune system and killing effect by antimicrobials. Laser-activated irrigation (LAI) and passive ultrasonic irrigation (PUI) have been proposed as alternative methods for cleaning and disinfecting the root canal, as an adjuvant to conventional chemo-mechanical preparation in order to improve debridement and disinfection. Nevertheless, the potential antibacterial effect of LAI using 0.5% of sodium hypochlorite (NaOCl) has received little attention. Glass Paste...
Source: Lasers in Medical Science - July 6, 2018 Category: Laser Surgery Source Type: research

Comparative study of the effect of Er:YAG and Er:Cr;YSGG lasers on porcelain: etching for the bonding of orthodontic brackets
This study investigated the effect of Er:YAG (smart 2940 Dplus, DEKA, Italy) and Er:CrYSGG (Waterlase iPlus, Biolase, USA) lasers on the shear bond strength (SBS) between the orthodontic brackets and dental porcelain in comparison with conventional acid etching with 9% hydrofluoric acid (HF, Ultradent, USA). A total of 60 specimens of maxillary incisor crown were prepared and randomly assigned to five groups; each group was subjected to a different porcelain surface conditioning: (1) etching with the 9% HF for 2  min; (2) etching with the 9% HF for 2 min followed by irradiation with the Er:CrYSGG laser (3-W power...
Source: Lasers in Medical Science - July 6, 2018 Category: Laser Surgery Source Type: research

Evaluating the effect of photobiomodulation with a 940-nm diode laser on post-operative pain in periodontal flap surgery
AbstractThe aim of this study was to evaluate the efficacy of low-power of 940-nm diode laser on post-operative pain after undisplaced flap surgery. This randomized clinical trial study was conducted using a split-mouth design. The study participants comprised 30 patients who needed periodontal flap surgery for periodontal pockets on the same tooth on both sides of the mandible. One side of the mandible was subjected to undisplaced flap surgery plus treatment with a 940-nm diode laser, and on the contralateral side, the surgery was conducted without applying the laser. Patients received anti-inflammatory medication and ana...
Source: Lasers in Medical Science - July 6, 2018 Category: Laser Surgery Source Type: research

Laser treatment contributes to maintain membrane integrity in stem cells from human exfoliated deciduous teeth (shed) under nutritional deficit
This study aimed to analyze the effects of laser irradiation on the membrane integrity and viability of stem cells from human exfoliated deciduous teeth (SHED) that were kept in serum starvation. Nutritional deficit was used to mimic the cellular stress conditions of SHED isolation for regenerative dental approaches, where laser therapy could be beneficial. SHED were cultured under serum starvation (MEM α + 1%FBS) for 1 or 24 h pre-irradiation (protocols A and B, respectively). Then, cells received low-level laser therapy (LLLT; 660 nm) at 2.5 J/cm2 (0.10  W; groups I and V), 5.0 J/cm2 (0.20 ...
Source: Lasers in Medical Science - July 6, 2018 Category: Laser Surgery Source Type: research

Qualitative evaluation of ferritin in serum samples by Raman spectroscopy and principal component analysis
AbstractIron molecule is of great importance in the synthesis of hemoglobin which is essential for oxygen transport. Iron levels are quantified by accurately high sensitivity tests, such as serum ferritin (SF). However, common studies to quantify SF are long and strenuous (~  5 h), for example enzyme-linked immunosorbent assay (ELISA). In this paper, blood serum samples were analyzed by Raman spectroscopy (RS), and a computational analysis of spectra is proposed to detect differences in SF as an alternative procedure. Serum samples were obtained from 22 patients, 9 w ho were clinically diagnosed with anemia and...
Source: Lasers in Medical Science - July 6, 2018 Category: Laser Surgery Source Type: research

Evaluation of scars in children after treatment with low-level laser
The objective is to analyze the effectiveness of low-level laser therapy on post-burn hypertrophic scar tissue in children. A randomized controlled study included 15 children, ranging from 2 to 10  years of age, presented with post-burn hypertrophic scars. They received He-Ne laser and topical treatment. Each scar was divided into two halves. One half was treated with laser therapy and topical treatment (study area), and the other half was treated with topical treatment only (control area). The children were evaluated before, and after 3 months of the study by Vancouver Scar Scale (VSS), ultrasonography, and lase...
Source: Lasers in Medical Science - July 4, 2018 Category: Laser Surgery Source Type: research

Photobiomodulation therapy reduces viral load and cell death in ZIKV-infected glioblastoma cell line
(Source: Lasers in Medical Science)
Source: Lasers in Medical Science - July 3, 2018 Category: Laser Surgery Source Type: research

Photodynamic therapy of balloon-injured rat carotid arteries using indocyanine green
AbstractPhotodynamic therapy (PDT) has been used to inhibit intimal hyperplasia in injured arteries. Because of the limited tissue penetration of visible light, an endovascular light source with a guided wire is often required for effective treatment. Indocyanine green (ICG), a near-infrared (NIR) photosensitizer, has been used in PDT for cancers. An extracorporeal light source may be used for shallow tissue because of the better tissue penetration of NIR light. The aim of this study was to evaluate the effect of ICG-PDT using extracorporeal NIR light on the inhibition of intimal hyperplasia in balloon-injured carotid arte...
Source: Lasers in Medical Science - June 16, 2018 Category: Laser Surgery Source Type: research

Correction to: Electroretinogram evaluation for the treatment of proliferative diabetic retinopathy by short-pulse pattern scanning laser panretinal photocoagulation
The published online version contains incorrect data in Table 2 caption. Argon should not be mentioned in the caption as this is not used in this paper. (Source: Lasers in Medical Science)
Source: Lasers in Medical Science - June 16, 2018 Category: Laser Surgery Source Type: research

Q-switched double frequency Nd:YAG 532-nm nanosecond laser vs. double frequency Nd:YAG 532-nm picosecond laser for the treatment of solar lentigines in Asians
The objective of the study is to compare the efficacy and safety in solar lentigines of Asian skin treated by Q-switched potassium titanyl phosphate (KTP) 532-nm nanosecond laser vs. KTP 532-nm picosecond laser for the treatment of solar lentigines in Asians. Thirty patients with at least 2 solar lentigines on the upper extremities were enrolled. A total of 30 paired lentiginous lesions were randomly treated with a single treatment of either Q-switched KTP 532-nm nanosecond laser vs. KTP 532-nm picosecond laser. In terms of efficacy, mean luminance score was evaluated at baseline, at 6th, and 12th week. Degree of pigment c...
Source: Lasers in Medical Science - June 15, 2018 Category: Laser Surgery Source Type: research

Treatment of mucositis with combined 660- and 808-nm-wavelength low-level laser therapy reduced mucositis grade, pain, and use of analgesics: a parallel, single-blind, two-arm controlled study
In conclusion, the current study demonstrated that a combination of red and infrared at a higher dose (300  J/cm2) reduced both oral mucositis grade and analgesics prescription. The effects of the combination of RT and LLLT are unclear and need more studies. (Source: Lasers in Medical Science)
Source: Lasers in Medical Science - June 15, 2018 Category: Laser Surgery Source Type: research

Promotion of wound healing through low-fluence ablative fractional laser treatment in diabetic mice
AbstractChronic ulcers are a significant cause of morbidity in diabetic patients, which can greatly affect a patient ’s quality of life. While numerous methods have been developed to promote and enhance wound healing in diabetic patients, a convenient, effective treatment for diabetic ulcers has yet to be established. Here, we demonstrate the promotion of wound healing using a low-fluence (2 mJ/spot) ablative f ractional laser (AFL) treatment in diabetic mice. Treatment was shown to confer increases in mRNA expression and in protein abundance of vascular endothelial growth factor (VEGF) and basic fibroblast grow...
Source: Lasers in Medical Science - June 14, 2018 Category: Laser Surgery Source Type: research

Diurnal variations and pulpal status: is there a need for FFT besides LDF?
AbstractThe aim of this article was to investigate the use of laser Doppler flowmetry (LDF) combined with a fast Fourier transformation (FFT). LDF data in relation to three different scenarios were evaluated: (a) LDF records of a right central upper incisor of one patient were used for FFT analysis. These records were obtained by means of 30 pre-manufactured splints, handled by dentists without any experience in LDF recording. (b) Diurnal variations in one patient were analysed with LDF and FFT using 11 splints by one and same experienced investigator at four specific moments of the day. (c) Pulpal status was analysed usin...
Source: Lasers in Medical Science - June 13, 2018 Category: Laser Surgery Source Type: research

Effects of laser therapy on patients who underwent rapid maxillary expansion; a systematic review
AbstractRapid maxillary expansion (RME) is one of the common treatments of transverse maxillary deficiency, and low-level laser therapy (LLLT) is one of the recommended solutions to enhance biological wound or bone healing. This review article aims to answer the following question: “What are the effects of LLLT, on patients who underwent surgical or non-surgical RME, in improving clinical success, wound healing, and bone regeneration?” A search in PubMed, Scopus, Web of Science, and ProQuest databases was performed, with a focus on the appropriate key words. Related articl es, up to May 2017, were screened, and...
Source: Lasers in Medical Science - June 12, 2018 Category: Laser Surgery Source Type: research

Thank you list 2017
(Source: Lasers in Medical Science)
Source: Lasers in Medical Science - June 12, 2018 Category: Laser Surgery Source Type: research

A novel Met-IR-782 near-infrared probe for fluorescent imaging-guided photothermal therapy in breast cancer
AbstractIn this work, a novel photothermal agent based on methionine (Met) was synthesized, which shows strong absorbance in the near infrared ray (NIR) region and is available for NIR imaging and in vivo photothermal therapy in a mouse model. Comparing to free IR-782, the obtained Met modified fluorescent dye (Met-IR-782) exhibited excellent fluorescence stability, preferable photothermal conversion efficiency under 780  nm laser irradiation and specific targeting to MCF7 (human breast adenocarcinoma cell line) cells. The fluorescence imaging ability enabled in situ monitoring of the tumor accumulation of Met-IR-782....
Source: Lasers in Medical Science - June 9, 2018 Category: Laser Surgery Source Type: research

Fibrotomy with diode laser (980  nm) and habit correlation in oral submucous fibrosis: a report of 30 cases
AbstractTo evaluate the efficacy of laser fibrotomy in patients with oral submucous fibrosis (OSMF) and also to correlate the habit variables causing OSMF. Thirty patients diagnosed clinically and histopathologically with OSMF were included in the study. Laser fibrotomy was done under local anesthesia using Zolar diode laser (980  nm frequency) at 2-W power in contact mode with fibro-optic cutting tip. The patients were advised to practice mouth-opening exercises rigorously, and topical corticosteroid was given for burning sensation and pain for 6 months. Pre- and post-treatment comparison of mouth opening, burni...
Source: Lasers in Medical Science - June 8, 2018 Category: Laser Surgery Source Type: research

Raman spectral feature selection using ant colony optimization for breast cancer diagnosis
AbstractPathology as a common diagnostic test of cancer is an invasive, time-consuming, and partially subjective method. Therefore, optical techniques, especially Raman spectroscopy, have attracted the attention of cancer diagnosis researchers. However, as Raman spectra contain numerous peaks involved in molecular bounds of the sample, finding the best features related to cancerous changes can improve the accuracy of diagnosis in this method. The present research attempted to improve the power of Raman-based cancer diagnosis by finding the best Raman features using the ACO algorithm. In the present research, 49 spectra wer...
Source: Lasers in Medical Science - June 4, 2018 Category: Laser Surgery Source Type: research

Gold nanorod-assisted near-infrared stimulation of bullfrog sciatic nerve
AbstractInfrared neural stimulation (INS) is a new and developing approach for neural repair, with the advantages of being non-contact, spatially precise, and artifact-free. However, the disadvantage of infrared light is that it is difficult to stimulate deep tissue because of its weak penetrating power. Therefore, this paper introduces an improved method using near-infrared laser to stimulate bullfrog sciatic nerves because of its strong penetrating power. Meanwhile, gold nanorods (Au NRs) are injected into the nerve to increase the absorption of light. The mechanism is the instantaneous temperature rise caused by the abs...
Source: Lasers in Medical Science - June 4, 2018 Category: Laser Surgery Source Type: research

In vitro comparison of an Er:YAG laser-activated bleaching system with different light-activated bleaching systems for color change, surface roughness, and enamel bond strength
AbstractTo compare an Er:YAG laser-activated bleaching system with different light-activated in-office bleaching systems for color change, surface roughness, and post-bleaching enamel bond strength. 51 enamel slabs were prepared from the sound buccal enamel of extracted bovine teeth. The teeth were randomly divided into three groups according to different light-activated office bleaching systems (n = 17): diode laser (Epic, Biolase) (940 nm, 7 W, continuous mode), Er:YAG laser (LightWalker, Fotona) (2940 nm, 50 mJ, 10 Hz, 1000 μs), and LED (Radii Plus) (440–480 nm, ...
Source: Lasers in Medical Science - June 2, 2018 Category: Laser Surgery Source Type: research

Near infrared laser irradiation induces NETosis via oxidative stress and autophagy
AbstractNETosis is a novel immune defense strategy in which neutrophil activation results in the formation of extracellular DNA/protein network which is able to kill microbial populations. NETosis can be induced in vitro by lipopolysaccharide (LPS) or phorbol myristate acetate (PMA). Due to the importance of NETosis in different physiological and pathological processes, photobiostimulation effect on this neutrophil activation mechanism has been investigated. Human granulocytes, isolated from venous blood of healthy donors, were stimulated with a diode laser emitting at 980  nm with an energy intensity ranging from 0 t...
Source: Lasers in Medical Science - June 2, 2018 Category: Laser Surgery Source Type: research

Monitoring system for investigating the effect of temperature change on optical properties
AbstractKnowledge about the changes in optical properties is needed for planning safer and more accurate laser treatments. A monitoring system was developed to study how the optical properties of a lipid emulsion are affected by temperature changes. A double-integrating-sphere system is modified with a controlled heating apparatus to measure the temperature-dependent diffuse reflectance and total transmittance values. The absorption and reduced scattering coefficients were estimated from the reflectance and transmittance values using an inverse adding-doubling method. The total transmittance showed positive correlation wit...
Source: Lasers in Medical Science - June 2, 2018 Category: Laser Surgery Source Type: research

Evaluation of the effects of Er:YAG laser, Nd:YAG laser, and two different desensitizers on dentin permeability: in vitro study
AbstractThe purpose of this in vitro study was to evaluate and compare the efficacy of erbium-doped yttrium aluminum garnet (Er:YAG) laser, neodymium-doped yttrium aluminum garnet (Nd:YAG) laser, PrevDent nano-hydroxyapatite toothpaste plus Repairing Serum Kit (PNH), and NUPRO Sensodyne Prophylaxis Paste with NovaMin (NPP) on dentin permeability reduction. Forty dentin discs obtained from bovine incisors were divided into four study groups: Er:YAG laser-treated (2940  nm; 0.2 W, 80 mJ/pulse, 3 Hz); Nd:YAG laser-treated (1064 nm; 1 W, 10 Hz); PNH-treated; and NPP-treated groups. The quanti...
Source: Lasers in Medical Science - May 30, 2018 Category: Laser Surgery Source Type: research

Effects of different photobiomodulation dosimetries on temporomandibular dysfunction: a randomized, double-blind, placebo-controlled clinical trial
AbstractChanges involving temporomandibular joint, masticatory musculature, and associated structures characterize temporomandibular dysfunction (TMD). The analgesic and anti-inflammatory effect produced by photobiomodulation has contributed to pain relief and functional improvement. However, the parameters to be used have not yet been well established. The aim of this study is to compare the efficacy of three different photobiomodulation dosimetries in the treatment of patients with TMD. A randomized, double-blind, placebo-controlled clinical trial with 44 subjects divided into the groups 8  J/cm2 (n = ...
Source: Lasers in Medical Science - May 30, 2018 Category: Laser Surgery Source Type: research

A randomized controlled trial comparing helium-neon laser therapy and infrared laser therapy in patients with diabetic foot ulcer
This study aimed to compare the effects of (HNLT) and (ILT) on diabetic foot ulcer. Sixty-five patients with diabetic foot ulcer (51 males and 14 females) aged 50 –60 years. The participants were classified randomly to two groups, groups I and II. Group I received helium-neon laser therapy (HNLT) and conventional therapy with and group II received infrared laser therapy (ILT) and conventional therapy with for 8 weeks. Ulcer surface area was assessed using a sheet of cellophane paper at the beginning of the study, after 4 weeks, and after 8 weeks at the end of the study. At the beginning of the stu...
Source: Lasers in Medical Science - May 30, 2018 Category: Laser Surgery Source Type: research

Application of cationized magnetoferritin for magnetic field-assisted delivery of short interfering RNA in vitro
AbstractCationized magnetoferritin is used for development of a simple, efficient, and fast delivery of short interference RNA into cells using combination of magnetophoresis for pre-concentration of siRNA-magnetoferritin complex on the surface of plated cells with subsequent application of nanosecond laser pulses producing stress waves in transfection chamber, which permeabilize cell membrane for the facilitated delivery of siRNA into the cell interior. As has been quantified using siRNA inducing cell death assay, by combination of these two physical factors we have obtained high efficiency for tested three different huma...
Source: Lasers in Medical Science - May 30, 2018 Category: Laser Surgery Source Type: research

Effect of photobiomodulation therapy on oxidative stress markers of gastrocnemius muscle of diabetic rats subjected to high-intensity exercise
This study aimed to determine whether photobiomodulation therapy (PBMT) in diabetic rats subjected to high-intensity exercise interferes with the expression of the oxidative stress marker in the gastrocnemius muscle. Twenty-four male Wistar rats were included in this study comprising 16 diabetic and eight control rats. The animals were allocated into three groups —control, diabetic fatigue, and diabetic PBMT fatigue groups. Diabetes was induced via the intraperitoneal administration of streptozotocin (50 mg/kg). We subsequently assessed blood lactate levels and PBMT. The animals of the diabetic fatigue group PBM...
Source: Lasers in Medical Science - May 28, 2018 Category: Laser Surgery Source Type: research

Foaming of blood in endovenous laser treatment
AbstractThis work is dedicated to a challenging issue of modern phlebology —establishment of a physical mechanism of the endovenous laser treatment (EVLT) against great saphenous vein incompetence (protuberant varicosities). Using optical and acoustical methods, we have studied the laser-induced formation of microbubbles in an aqueous solution of surface-active substance s, serum, and blood directly in patients while conducting EVLT of the great saphenous vein in a clinical setting. We have used lasers with wavelengths 0.97 and 1.47 μm. Their radiation was transmitted through a quartz-quartz polymer fiber 600...
Source: Lasers in Medical Science - May 28, 2018 Category: Laser Surgery Source Type: research

Treatment of intravitreal bevacizumab combined with focal laser photocoagulation in the case of macular telangiectasia type 2 with retinal arterial macroaneurysm
AbstractMacular telangiectasia type 2 (Mac Tel 2) is a bilateral disease of unknown cause with characteristic changes of the retinal vasculature. To the best of our knowledge, we could not find any reported cases of Mac Tel 2 with retinal arterial macroaneurysm (RAM). Our aim is to report a case of Mac Tel 2 with RAM. (Source: Lasers in Medical Science)
Source: Lasers in Medical Science - May 26, 2018 Category: Laser Surgery Source Type: research

Enhancing the effects of chemotherapy by combined macrophage-mediated photothermal therapy (PTT) and photochemical internalization (PCI)
AbstractLight-based treatment modalities such as photothermal therapy (PTT) or photochemical internalization (PCI) have been well documented both experimentally and clinically to enhance the efficacy of chemotherapy. The main purpose of this study was to examine the cytotoxic effects of silica –gold nanoshell (AuNS)-loaded macrophage-mediated (MaNS) PTT and bleomycin BLM-PCI on monolayers of squamous cell carcinoma cells. The two modalities were applied separately and in simultaneous combination. Two different wavelengths of light were employed simultaneously, one to activate a highly efficient PCI photosensitizer, A...
Source: Lasers in Medical Science - May 26, 2018 Category: Laser Surgery Source Type: research

Comment on “Efficacy of photodynamic therapy as adjunct treatment of chronic periodontitis: a systematic review and meta-analysis”
(Source: Lasers in Medical Science)
Source: Lasers in Medical Science - May 26, 2018 Category: Laser Surgery Source Type: research

Type 2 diabetes detection based on serum sample Raman spectroscopy
AbstractIn this work, we propose to the Raman spectroscopy as a new technique for the detection of the type 2 diabetes using blood serum samples. The serum samples were obtained from 15 patients who were clinically diagnosed with type 2 diabetes mellitus and 20 healthy volunteers. The average spectra showed equally intense peaks as, 695 cm−1, the doublet of tyrosine at 828 and 853 cm−1, phenylalanine at 1002 and 1028 cm−1, the phospholipid shoulder at 1300 –1345 cm−1, and proteins (amide I) at 1654 cm−1. The major differences were found at 661 and 1404 cm−1 (glutathione), 714 (poly...
Source: Lasers in Medical Science - May 25, 2018 Category: Laser Surgery Source Type: research

Efficacy of low-level laser therapy in management of recurrent herpes labialis: a systematic review
AbstractRecurrent herpes labialis (RHL) is a highly prevalent viral infection that affects the oro-facial region. Current treatment options have limited efficacy in reducing healing time and recurrence rate of the disease. Recently, low-level laser therapy has been proposed as a potential treatment alternative for the management of RHL with no side effects. This systematic review aims to evaluate the effectiveness of laser therapy in the management and prevention of RHL. A comprehensive search of Medline/PubMed, Scopus, and Web of Science was carried out to identify published clinical trials comparing laser intervention to...
Source: Lasers in Medical Science - May 25, 2018 Category: Laser Surgery Source Type: research

Interleukin-10 and collagen type II immunoexpression are modulated by photobiomodulation associated to aerobic and aquatic exercises in an experimental model of osteoarthritis
AbstractThe aim of this study was to compare the effects of photobiomodulation (PBM) associated with an aerobic and an aquatic exercise training on the degenerative process related to osteoarthritis (OA) in the articular cartilage in rats. Fifty male Wistar rats were randomly divided into 5 groups: OA control group (CG), OA plus aerobic training group (AET), OA plus aquatic training group (AQT), OA plus aerobic training associated with PBM group (AETL), OA plus aquatic training associated with PBM group (AQTL). The aerobic training (treadmill; 16  m/min; 50 min/day) and the aquatic training (water jumping; 50&nda...
Source: Lasers in Medical Science - May 24, 2018 Category: Laser Surgery Source Type: research

Effects of meta-tetrahydroxyphenylchlorin photodynamic therapy on isogenic colorectal cancer SW480 and SW620 cells with different metastatic potentials
AbstractThe aim of this study is to investigate the antitumor effects and possible mechanisms of meta-tetrahydroxyphenylchlorin-mediated photodynamic therapy (m-THPC-PDT) on human primary (SW480) and metastatic (SW620) colon cancer cell lines. SW480 and SW620 cells were incubated with various concentrations of m-THPC, followed by photodynamic irradiation. Subcellular localization of m-THPC in cells was observed with confocal laser scanning microscopy (CLSM). Photocytotoxicity of m-THPC in the two cells was investigated by using MTT assay. The flow cytometry was employed to detect the cell apoptosis. The migration and long-...
Source: Lasers in Medical Science - May 24, 2018 Category: Laser Surgery Source Type: research

Effect of photobiomodulation (670  nm) associated with vitamin A on the inflammatory phase of wound healing
AbstractWound healing is a complex biological process with specific phases. Photobiomodulation (PBM) decreases the inflammatory infiltrate, stimulating fibroblast proliferation and angiogenesis, and therefore, is indicated for wound healing. Vitamin A is used to reverse the inhibitory effects on wound healing and accelerate the healthy granulation tissue. The study aimed to evaluate the effect of topical vitamin A and PBM (GaAlAs) in inflammatory phase of cutaneous wounds. Forty Wistar male rats were separated into four groups: (1) control (CG); (2) laser group (LG) GaAlAs, 670  nm, 30 mW, energy per point of 0.9...
Source: Lasers in Medical Science - May 22, 2018 Category: Laser Surgery Source Type: research

Evaluation of a nanocomposite of PEG-curcumin-gold nanoparticles as a near-infrared photothermal agent: an in vitro and animal model investigation
AbstractHyperthermia is a promising alternative modality for the conventional cancer treatments. Nanoparticle-mediated photothermal therapy (PTT) has been widely applied for hyperthermia cancer therapy by a near-infrared light irradiation. Some special nanoparticles can convert light energy into heat and destroy the tumor cells. Inspired from the photothermal efficacy of the gold nanoparticles, here we synthesized, characterized, and applied novel photothermal polyethylene glycol-curcumin-gold nanoparticles (PEG-Cur-Au NPs) in cancer PTT. The effect of PEG-Cur-Au NPs upon irradiation by an 808-nm laser on C540 (B16/F10) ce...
Source: Lasers in Medical Science - May 22, 2018 Category: Laser Surgery Source Type: research

Ability of blue laser imaging with magnifying endoscopy for the diagnosis of gastric intestinal metaplasia
AbstractWe aimed to determine the utility of blue laser imaging (BLI) with magnifying endoscopy (BLI-ME) for the prediction and diagnosis of gastric intestinal metaplasia (GIM). Participants, aged between 40 and 75  years, undergoing gastroscopy from January to April 2017 were included in this study. The ability of BLI-ME and white light endoscopy (WLE) to detect GIM was assessed by comparing the endoscopic findings with the histological findings. The correlation between the grades of light blue crest (LBC) a ppearance and histology grade of GIM was calculated. We included 100 participants in this study. GIM was diagn...
Source: Lasers in Medical Science - May 18, 2018 Category: Laser Surgery Source Type: research

Laser reduction of specific microorganisms in the periodontal pocket using Er:YAG and Nd:YAG lasers: a randomized controlled clinical study
The objective of this study was to evaluate the microbiological and clinical outcomes following nonsurgical treatment by either scaling and root planing, combination of Nd:YAG and Er:YAG lasers, or by Er:YAG laser treatment alone. The study involved 60 patients with generalized chronic periodontitis, randomly assigned into one of three treatment groups of 20 patients. The first group received scaling and root planing by hand instruments (SRP group), the second group received Er:YAG laser treatment alone (Er group), and the third group received combined treatment with Nd:YAG and Er:YAG lasers (NdErNd group). Microbiological...
Source: Lasers in Medical Science - May 15, 2018 Category: Laser Surgery Source Type: research

Less is more: similar efficacy in three sessions and seven sessions of pulsed dye laser treatment in infantile port-wine stain patients
AbstractPort-wine stains (PWS) affect 0.3 to 0.5% of newborns and pulsed dye laser (PDL) remains the treatment of choice. However, no reliable study regarding the benefits of more frequent has been conducted. We designed the present study to evaluate whether more frequent PDL treatments in infantile patients would achieve further lightening of erythema. We prospectively investigated 20 infants with PWS. Two adjacent sites were both treated for a 12-week duration and randomly allocated to be treated for seven sessions at 2-week intervals or three sessions at 6-week intervals. The efficacy outcome 2  months after the fi...
Source: Lasers in Medical Science - May 10, 2018 Category: Laser Surgery Source Type: research

Indocyanine green-mediated photobiomodulation on human osteoblast cells
AbstractPhotobiomodulation (PBM) and photodynamic therapy (PDT) share similar mechanisms but have opposite aims. Increased levels of reactive oxygen species (ROS) in the target tissue in response to light combined photosensitizer (PS) application may lead to cell proliferation or oxidative damage depending on the ROS amount. The purpose of the present study is to investigate the effect of indocyanine green (ICG)-mediated PBM on osteoblast cells by measuring cell viability, proliferation, alkaline phosphatase (ALP) activity, mineralization, and gene expressions of three phenotypic osteoblast markers. A diode laser irradiati...
Source: Lasers in Medical Science - May 9, 2018 Category: Laser Surgery Source Type: research

Bactericide effect of methylene blue associated with low-level laser therapy in Escherichia coli bacteria isolated from pressure ulcers
AbstractThe present study analyzed the bactericidal effect of methylene blue associated with low-level lasers onEscherichia coli isolated from a pressure ulcer. Microbiological material from a pressure ulcer was isolated using an aseptic swab, and antimicrobial activity was verified using the diffusion disc method. Methylene blue was used at concentrations of 0.001 and 0.005%, and low-level lasers of 670, 830, and 904  nm, with the energy densities of 4, 8, 10, and 14 J/cm2, were tested on three plates each and combined with methylene blue of each concentration. In addition, three control plates were used, with e...
Source: Lasers in Medical Science - May 9, 2018 Category: Laser Surgery Source Type: research