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Comment on “Low-level laser therapy with 940 nm diode laser on stability of dental implants: a randomized controlled clinical trial”
(Source: Lasers in Medical Science)
Source: Lasers in Medical Science - December 16, 2017 Category: Laser Surgery Source Type: research

EMLA cream does not influence efficacy and pain reduction during pulsed-dye laser treatment of port-wine stain: a prospective side-by-side comparison
AbstractEMLA cream was developed to reduce pain during pulsed-dye laser (PDL) treatment; however, no standard assessment for the therapeutic outcomes of PDL with EMLA creams thus far available. This comparative, prospective clinical trial evaluates laser efficacy and pain reduction during PDL treatment with EMLA cream for local topical anesthesia. Nineteen patients with untreated port-wine stain (PWS) were treated using PDL and examined in this study. Treatment specifications included Vbeam ® PDL (Candela Corp.), 595-nm wavelength, 9 J/cm2 radiant exposure, 0.45  ms pulse duration, 10 mm spot size, and c...
Source: Lasers in Medical Science - December 15, 2017 Category: Laser Surgery Source Type: research

Infrared photobiomodulation (PBM) therapy improves glucose metabolism and intracellular insulin pathway in adipose tissue of high-fat fed mice
AbstractObesity represents a continuously growing global epidemic and is associated with the development of type 2 diabetes mellitus. The etiology of type 2 diabetes is related to the resistance of insulin-sensitive tissues to its action leading to impaired blood glucose regulation. Photobiomodulation (PBM) therapy might be a non-pharmacological, non-invasive strategy to improve insulin resistance. It has been reported that PBM therapy in combination with physical exercise reduces insulin resistance. Therefore, the aim of this study was to investigate the effects of PBM therapy on insulin resistance in obese mice. Male Swi...
Source: Lasers in Medical Science - December 15, 2017 Category: Laser Surgery Source Type: research

Antimicrobial photodynamic therapy —a promising treatment for prosthetic joint infections
This study investigated Photodynamic Therapy (PDT) as a means of eradicating bacteria that cause PJI, using a laser with a 665-nm wavelength and methylene blue (MB) as the photosensitizer. The effectiveness of MB concentration on the growth inhibition of methicillin-sensitiveStaphylococcus aureus (MSSA), methicillin-resistantStaphylococcus aureus (MRSA),Staphylococcus epidermidis,Pseudomonas aeruginosa andAcinetobacter baumannii was investigated. The effect of laser dose was also investigated and the optimized PDT method was used to investigate its bactericidal effect on species within planktonic culture and following the ...
Source: Lasers in Medical Science - December 15, 2017 Category: Laser Surgery Source Type: research

Effect of 1064-nm Q-switched Nd:YAG laser on invasiveness and innate immune response in keratinocytes infected with Candida albicans
AbstractCandida albicans is an opportunistic pathogen commensal in the oral cavity, vagina, and healthy skin. Common therapeutic options for fungal infections are topical or systemic antifungal drugs. Recently, in cutaneous pathologies, lasers and light-based treatments have emerged showing few contraindications and minimal side effects. The Q-switched (Nd-YAG) laser at a wavelength of 1064  nm has been shown to be useful in dermatology, dentistry, and some other medical specialties. It is used to treat onychomycoses, warts, and wounds and in some other treatments. We analyzed the effect of Q-switched (Nd-YAG) laser 1...
Source: Lasers in Medical Science - December 14, 2017 Category: Laser Surgery Source Type: research

Comparison between whole-body and head and neck neurovascular coils for 3-T magnetic resonance proton resonance frequency shift thermography guidance in the head and neck region
AbstractThe purpose of this study is to compare the image quality of magnetic resonance (MR) treatment planning images and proton resonance frequency (PRF) shift thermography images and inform coil selection for MR-guided laser ablation of tumors in the head and neck region. Laser ablation was performed on an agar phantom and monitored via MR PRF shift thermography on a 3-T scanner, following acquisition of T1-weighted (T1W) planning images. PRF shift thermography images and T2-weighted (T2W) planning images were also performed in the neck region of five normal human volunteers. Signal-to-noise ratios (SNR) and temperature...
Source: Lasers in Medical Science - December 9, 2017 Category: Laser Surgery Source Type: research

Efficacy of pulsed dye laser treatment for common warts is not influenced by the causative HPV type: a prospective study
The objective of this prospective blinded study was to assess whether the HPV subtype was predictive of response to PDL. For that matter, 26 verrucae from 26 immunocompetent patients were biopsied prior to treatment by PDL. HPV coding sequences were isolated and genotyped using PCR analysis. Patients were treated by PDL (595  nm wavelength, 5 mm spot size, 1.5 ms pulse duration, 12 J/cm2 fluence) once a month for up to 6  months, and clinical response was assessed. Binary logistic regression analysis and linear logistic regression analysis were used in order to evaluate statistical significance. Di...
Source: Lasers in Medical Science - December 7, 2017 Category: Laser Surgery Source Type: research

Direct biological effects of fractional ultrapulsed CO 2 laser irradiation on keratinocytes and fibroblasts in human organotypic full-thickness 3D skin models
We present the first study addressing molecular effects of fractional non-sequential ultrapulsed CO2 laser treatment using a 3D skin model that allows standardized investigations of time-dependent molecular changes ex vivo. While histological examination was performed to assess morphological changes, we utilized gene expression profiling using microarray and qRT-PCR analyses to identify molecular effects of laser treatment. Irradiated models exhibited dose-dependent morphological changes resulting in an almost complete recovery of the epidermis 5  days after irradiation. On day 5 after laser injury with a laser fluenc...
Source: Lasers in Medical Science - December 7, 2017 Category: Laser Surgery Source Type: research

Laser surface modification of decellularized extracellular cartilage matrix for cartilage tissue engineering
AbstractThe implantation of autologous cartilage as the gold standard operative procedure for the reconstruction of cartilage defects in the head and neck region unfortunately implicates a variety of negative effects at the donor site. Tissue-engineered cartilage appears to be a promising alternative. However, due to the complex requirements, the optimal material is yet to be determined. As demonstrated previously, decellularized porcine cartilage (DECM) might be a good option to engineer vital cartilage. As the dense structure of DECM limits cellular infiltration, we investigated surface modifications of the scaffolds by ...
Source: Lasers in Medical Science - December 6, 2017 Category: Laser Surgery Source Type: research

Non-specific effects and clusters of women with painful TMD responders and non-responders to LLLT: double-blind randomized clinical trial
AbstractThe aim was to analyze the non-specific effects (placebo, spontaneous remission, and regression to the mean) of the low-level laser therapy (LLLT) in women with myofascial pain (painful temporomandibular disorder (TMD)), as well as to differentiate between responders and non-responder clusters to active and placebo LLLT according to the anxiety levels, salivary cortisol, use of oral contraceptives, and premenstrual period. Sixty-four women diagnosed with myofascial pain (Research Diagnostic Criteria for Temporomandibular Disorders (RDC/TMD)) were included, divided into laser (n = 20), placebo group (n...
Source: Lasers in Medical Science - December 5, 2017 Category: Laser Surgery Source Type: research

Photobiomodulation therapy protects skeletal muscle and improves muscular function of mdx mice in a dose-dependent manner through modulation of dystrophin
This study aimed to analyze the protective effects of photobiomodulation therapy (PBMT) with combination of low-level laser therapy (LLLT) and light emitting diode therapy (LEDT) on skeletal muscle tissue to delay dystrophy progression in mdx mice (DMDmdx). To this aim, mice were randomly divided into five different experimental groups: wild type (WT), placebo-control (DMDmdx), PBMT with doses of 1  J (DMDmdx), 3  J (DMDmdx), and 10  J (DMDmdx). PBMT was performed employing a cluster probe with 9 diodes (1 x 905nm super-pulsed laser diode; 4 x 875nm infrared LEDs; and 4 x 640nm red LEDs, manufactured by Mult...
Source: Lasers in Medical Science - December 5, 2017 Category: Laser Surgery Source Type: research

Is photobiomodulation (PBM) effective for the treatment of dentin hypersensitivity? A systematic review
AbstractThe present study aims to evaluate the current scientific data regarding the effectiveness of photobiomodulation (PBM) in the treatment of dentin hypersensitivity (DH) as an alternative method for pain control. A systematic review was conducted to assess the effectiveness of PBM as treatment for DH. A complete literature search was performed up to October 2016. Searches were conducted using Boolean operators and MeSH terms. References of all selected full-text articles and related reviews were scanned. A total of 280 articles were identified (241 articles were excluded by the title and abstract). Of the 39 articles...
Source: Lasers in Medical Science - December 5, 2017 Category: Laser Surgery Source Type: research

Muscle fiber conduction velocity and EMG amplitude of the upper trapezius muscle in healthy subjects after low-level laser irradiation: a randomized, double-blind, placebo-controlled, crossover study
AbstractAlthough low-level laser therapy (LLLT) is an important resource for the treatment of non-specific neck pain patients, the dose which presents the greatest therapeutic potential for the treatment of this pathology is still unclear. The present study aimed to evaluate the immediate effect of LLLT on the muscle fiber conduction velocity (MFCV) and electromyographic activity (EMG) of the upper trapezius (UT) muscle in healthy individuals. A total of 20 healthy subjects were enrolled in a randomized, double-blind, crossover study. Active LLLT (820  nm wavelength, 30 mW, energy total 18 J) or placebo LLLT...
Source: Lasers in Medical Science - December 5, 2017 Category: Laser Surgery Source Type: research

Low-intensity LED therapy (658  nm) on burn healing: a series of cases
In conclusion, there was a faster clinical improvement in the irradiated limbs. (Source: Lasers in Medical Science)
Source: Lasers in Medical Science - December 4, 2017 Category: Laser Surgery Source Type: research

Laser for bone healing after oral surgery: systematic review
AbstractThe purpose of this study is to perform a systematic review on the use of lasers in oral surgery for bone healing. Selection of articles was carried out by two evaluators in Pubmed and Web of Science databases for published articles and OpenGray for gray literature. Search strategy was developed based on the PICO Question “Does the use of lasers after oral surgery improve bone healing?”. Eligibility criteria were: being on laser; evaluate bone healing; involve oral surgery; do not be about implant, periodontics, orthodontics, osteonecrosis or radiotherapy, nor revisions, clinical cases, etc. Data were c...
Source: Lasers in Medical Science - December 1, 2017 Category: Laser Surgery Source Type: research

Chondroitin sulfate and glucosamine sulfate associated to photobiomodulation prevents degenerative morphological changes in an experimental model of osteoarthritis in rats
This study showed that CS/Gl associated with PBM was effective in modulating inflammatory process and preventing the articular tissue degradation in the knees OA rats. (Source: Lasers in Medical Science)
Source: Lasers in Medical Science - December 1, 2017 Category: Laser Surgery Source Type: research

Comment on “Effects of the GaAlAs diode laser (780 nm) on the periodontal tissues during orthodontic tooth movement in diabetes rats: histomorphological and immunohistochemical analysis”
(Source: Lasers in Medical Science)
Source: Lasers in Medical Science - December 1, 2017 Category: Laser Surgery Source Type: research

Comparing aspheric ablation profile with standard corneal ablation for correction of myopia and myopic astigmatism, a contralateral eye study
AbstractThe purpose of this study is to compare visual outcomes of myopic refractive surgery, using tissue saving (TS), a standard ablation profile by a Technolas 217z100, and aberration smart ablation (ASA), an aspheric algorithm by a MEL 80 excimer laser in two eyes of one patient. In this prospective interventional paired-eye controlled study, a total of 100 eyes of 50 participants with myopia with or without astigmatism underwent thin-flap Femto-LASIK, using a Femto LDV femtosecond laser (Ziemer Ophthalmic Systems AG, Port, Switzerland). For each patient, one eye was randomly treated with tissue-saving algorithm (TS gr...
Source: Lasers in Medical Science - November 8, 2017 Category: Laser Surgery Source Type: research

Effects of photobiomodulation on experimental models of peripheral nerve injury
AbstractPhototherapy has demonstrated positive effects in the treatment of peripheral nerve injury, but there is a need to investigate the dosimetric parameters. Thus, the aim of the present study was to conduct a literature review on the effects of photobiomodulation with the use of low-level laser therapy (LLLT) on the treatment of peripheral nerve injury in experimental models. The databases of PubMed/MEDLINE, SCOPUS, and SPIE Digital Library were searched for articles on the use of LLLT in experimental models of peripheral nerve injury published in English between January 2007 and March 2016. The laser parameter variab...
Source: Lasers in Medical Science - November 8, 2017 Category: Laser Surgery Source Type: research

Comparison between performance of single-fiber reflectance spectroscopy (SFRS) system and colposcopy: a phase III trial
In conclusion, using the online, simple and non-invasive SFRS system to choose between several suspicious sites in a patient in the clinic may be recommended. (Source: Lasers in Medical Science)
Source: Lasers in Medical Science - November 8, 2017 Category: Laser Surgery Source Type: research

Anti-inflammatory effects of low-level laser therapy on human periodontal ligament cells: in vitro study
AbstractPeriodontal disease is a chronic inflammatory disease that is commonly treated with surgical and nonsurgical techniques. However, both approaches have limitations. Low-level laser therapy (LLLT) has been widely applied in reducing inflammatory reactions, and research indicates that LLLT induces an anti-inflammatory effect that may enhance periodontal disease therapy. The purpose of this study was to investigate the anti-inflammatory effect of LLLT on human periodontal ligament cells (hPDLCs) in an inflammatory environment and aimed to determine the possible mechanism of action. Cells were cultured and treated with ...
Source: Lasers in Medical Science - November 7, 2017 Category: Laser Surgery Source Type: research

The effect of laser-assisted hatching on pregnancy outcomes of cryopreserved-thawed embryo transfer: a meta-analysis of randomized controlled trials
AbstractIt is well known that laser-assisted hatching (LAH) is the most popular and ideal embryo hatching technology, but the relevance to pregnancy outcomes of cryopreserved-thawed embryo transfer (ET) is controversial. The purpose of this meta-analysis was to evaluate the effects of LAH on pregnancy outcomes of cryopreserved-thawed ET. We searched for relevant studies published in the PubMed, EMBASE, and Cochrane Central databases up to March 2017. This meta-analysis was primarily used to evaluate the effect of laser-assisted hatching on assisted reproductive outcomes: clinical pregnancy, embryo implantation, multiple pr...
Source: Lasers in Medical Science - November 7, 2017 Category: Laser Surgery Source Type: research

The effect of the KTP laser on smear layer and temperature change: an in vitro study
AbstractThe purpose of this study was to evaluate the effects of potassium-titanyl-phosphate (KTP) laser irradiation on smear layer removal and temperature changes of the root surfaces. Two hundred four extracted single-root human premolars were included. The canals were instrumented and divided into two main groups: group A (smear layer examinationn:119) and group B (temperature change examination n:85). Each group was divided into subgroups (n:17) according to the different five laser power settings: A1, B1: 1  W–5.33 J/cm2; A2, B2: 1.5  W–7.52 J/cm2; A3, B3: 2  W–10.3 J/cm...
Source: Lasers in Medical Science - November 6, 2017 Category: Laser Surgery Source Type: research

Laser acupuncture-induced analgesic effect and molecular alterations in an incision pain model: a comparison with electroacupuncture-induced effects
AbstractLow-level laser acupuncture (LLLA) produces photobiomodulation through acupuncture point and is an alternative to low-level laser therapy. Although the analgesic effect of LLLA on chronic pain has been proven, its effect on acute postincisional pain has yet to be investigated. A plantar incision (PI) model was used to mimic human postsurgical pain. Male adult rats received GaAlAs laser irradiation at the right ST36 acupoint immediately after operation and on the following 4  days. Three laser treatment groups (two red laser groups with a 30- or 15-min treatment duration and one 30-min near-infrared laser group...
Source: Lasers in Medical Science - November 4, 2017 Category: Laser Surgery Source Type: research

Laser-induced autofluorescence-based objective evaluation of burn tissue repair in mice
AbstractManagement of burn injuries are a growing concern, especially in determining the progression of healing. Several techniques are being practiced in clinics and have been considered all-time standard approaches to determine pre- and post-treatment outcomes of a healthy healing. However, these kinds of methods involve repeated biopsies and thereby hindering tissue repair. In view of this, our perspective was to develop a non-invasive tool in an attempt to provide a solution to determine the progression of healing, in vivo. Hence, the present study was designed to investigate the ability of laser-induced fluorescence (...
Source: Lasers in Medical Science - November 3, 2017 Category: Laser Surgery Source Type: research

Dose-response effect of photobiomodulation therapy on neuromuscular economy during submaximal running
AbstractThe purpose of this study was to verify the photobiomodulation therapy (PBMT) effects with different doses on neuromuscular economy during submaximal running tests. Eighteen male recreational runners participate in a randomized, double-blind, and placebo-controlled trial, which each participant was submitted to the same testing protocol in five conditions: control, placebo, and PBMT with doses of 15, 30, and 60  J per site (14 sites in each lower limb). The submaximal running was performed at 8 and 9 km h−1 during 5  min for each velocity. Muscle activation of thevastus lateralis (VL),vast...
Source: Lasers in Medical Science - November 3, 2017 Category: Laser Surgery Source Type: research

Low-level laser therapy prevents endothelial cells from TNF- α/cycloheximide-induced apoptosis
AbstractLow-level laser therapy (LLLT), widely used in physiotherapy, has been known to enhance wound healing and stimulate cell proliferation, including fibroblast and endothelial cells. Applying LLLT can increase cell proliferation in many kinds of cells including fibroblasts and endothelial cells. However, the protective mechanisms of LLLT on endothelial apoptosis remain unclear. We hypothesized LLLT can protect endothelial cells from inflammation-induced apoptosis. Human endothelial cell line, EA.hy926 cells, and TNF- α/cycloheximide (TNF/CHX) were used to explore the protective effects of LLLT (660 nm) on i...
Source: Lasers in Medical Science - November 3, 2017 Category: Laser Surgery Source Type: research

Picosecond 532-nm neodymium-doped yttrium aluminium garnet laser —a novel and promising modality for the treatment of café-au-lait macules
The objective of this study is to report our experience in treating CALMs using a picosecond 532-nm neodymium-doped yttrium aluminium garnet (PS 532 nm) laser. This is a retrospective case series of 16 patients with CALMs who were treated by a PS 532-nm laser (1–4 treatments, 4–8 weeks apart). Response as seen on clinical photographs was assessed by two independent dermatologists and graded on a scale of 0 (exacerbation) to 5 (95– 100% improvement). Patient satisfaction and tolerance were documented at final visit. The results of 15 patients demonstrated significant improvement (average 3.43), a...
Source: Lasers in Medical Science - November 2, 2017 Category: Laser Surgery Source Type: research

Human adipose-derived stem cell spheroid treated with photobiomodulation irradiation accelerates tissue regeneration in mouse model of skin flap ischemia
AbstractSkin flap grafting is a form of transplantation widely used in plastic surgery. However, ischemia/reperfusion injury is the main factor which reduces the survival rate of flaps following grafting. We investigated whether photobiomodulation (PBM) precondition prior to human adipose-derived stromal cell (hASC) spheroid (PBM-spheroid) transplantation improved skin tissue functional recovery by the stimulation of angiogenesis and tissue regeneration in skin flap of mice. The LED had an emission wavelength peaked at 660  ± 20 nm (6 J/cm2, 10  mW/cm2). The expression of angiogenic growth f...
Source: Lasers in Medical Science - October 23, 2017 Category: Laser Surgery Source Type: research

Treatment of keloid scars using light-, laser- and energy-based devices: a contemporary review of the literature
AbstractKeloid scars are common and have a predilection for young, ethnic skin often with a family history. Keloids can be painful and pruritic and cause significant emotional distress when particularly visible or prominent. In this article, we review the evidence underlying the use of laser- and energy-based devices for treatment of keloid scars, either as monotherapy or in conjunction with other therapies such as corticosteroids, surgery and silicone gel in the treatment of keloid scars. (Source: Lasers in Medical Science)
Source: Lasers in Medical Science - October 18, 2017 Category: Laser Surgery Source Type: research

In vitro bond strength of an epoxy resin-based root canal sealer to root dentin irradiated with high-power lasers and adhesive interface analyses
AbstractThe aim of this study was to evaluate in vitro the influence of high-power lasers (Nd:YAG and diode 980  nm) associated with mineral coal as fotopotencializer on bond strength of an epoxy resin-based root canal sealer to root dentin, using the pushout test, and on the dentin/filling material interface, using confocal laser microscopy. For this purposes, 50 canines were instrumented with Mtwo rotary s ystem up to #50.04 instrument and randomly assigned to five groups (n = 10): group I—control EDTAC; group II—EDTAC and Nd:YAG laser; group III—EDTAC and diode laser 980 nm; group I...
Source: Lasers in Medical Science - October 18, 2017 Category: Laser Surgery Source Type: research

Enhanced photoinduced antibacterial activity of a BODIPY photosensitizer in the presence of polyamidoamines
AbstractPhotosensitizers belonging to the boron-dipyrromethenes (BODIPYs) class were recently found endowed with good efficacy in the antibacterial photodynamic therapy (aPDT) against both Gram-positive and Gram-negative bacteria. In this paper, we report on the remarkable adjuvant effect exerted in this respect by linear polyamidoamines (PAAs), a family of moderately basic polymers obtained by Michael-type polyaddition of amines to bisacrylamides. Three different PAAs (AGMA1, BP-AGMA, and BP-DMEDA) were studied, testing for each two different molecular weight samples (8000 and 24000 Da). At nontoxic concentrations (1 or 1...
Source: Lasers in Medical Science - October 17, 2017 Category: Laser Surgery Source Type: research

Microvascular imaging and monitoring of human oral cavity lesions in vivo by swept-source OCT-based angiography
We report the development of optical coherence tomography- (OCT) based angiography (OCTA) to image blood flow within microcirculatory tissue beds in human oral cavity in vivo with a field of view at 10  mm × 10 mm. Three-dimensional (3D) structural and vascular images of labial mucosa tissue are obtained at a single 3D acquisition. Pathologic mucosal sites with mouth ulcers are examined using the OCT tomograms and angiograms, upon which to monitor the lesion healing process over a period of 2  weeks. Quantitative metrics of the capillary loop density within the lamina propria layer are evalua...
Source: Lasers in Medical Science - October 16, 2017 Category: Laser Surgery Source Type: research

Abstracts Laser Florence 2017
(Source: Lasers in Medical Science)
Source: Lasers in Medical Science - October 16, 2017 Category: Laser Surgery Source Type: research

Surface roughness and bacterial adhesion on root dentin treated with diode laser and conventional desensitizing agents
AbstractThe treatments for dentin hypersensitivity (DH) may change the surface roughness of the root dentin, which can lead to biofilm accumulation, increasing the risk of root caries. The aim was to compare the surface roughness of root dentin after different treatments of DH and the biofilm formation on those surfaces. After initial surface roughness (Sa) assessment, 50 bovine root fragments received the following treatments (n = 10): G 1—no treatment; G2—5% sodium fluoride varnish; G3—professional application of a desensitizing dentifrice; G4—toothbrushing with a desensitizing dentifric...
Source: Lasers in Medical Science - October 14, 2017 Category: Laser Surgery Source Type: research

Analysis of choroidal thickness in ocular hypertensive patients using enhanced depth imaging optical coherence tomography
This study aimed to compare choroidal thickness between subjects with ocular hypertension (OHT) and normal individuals and explore factors affecting choroidal thickness. This study included 60 untreated newly diagnosed OHT eyes and 60 normal eyes. Choroidal thickness obtained from Cirrus HD-OCT was measured at different locations in the macular and peripapillary regions and compared between the two groups before and after adjusting for potential confounding variables. Regression analysis was performed to figure out factors influencing choroidal thickness. The macular choroidal thickness did not vary significantly between O...
Source: Lasers in Medical Science - October 13, 2017 Category: Laser Surgery Source Type: research

Low-intensity LED therapy ( λ 640 ± 20 nm) on saphenectomy healing in patients who underwent coronary artery bypass graft: a randomized, double-blind study
The objective of the present study was to evaluate clinically the saphenous tissue repair after LED therapy. Forty subjects of both genders who underwent CABG with extracorporeal circulation were randomly di vided into two groups: the placebo (PG) and experimental (EG). The experimental group underwent low-intensity LED therapy (λ 640  ± 20 nm, 6 J/cm2) on saphenectomy. The tissue repair was analyzed by digital photogrammetry on the first and fifth postoperative day. The border closure was blindly evaluated by three researchers. The hematoma and hyperemia area was quantitatively analyzed u...
Source: Lasers in Medical Science - October 13, 2017 Category: Laser Surgery Source Type: research

Low-level laser irradiation induces in vitro proliferation of stem cells from human exfoliated deciduous teeth
AbstractThe aim of this study was to evaluate the effect of low-level laser irradiation (LLLI) on the proliferation and viability of stem cells from human exfoliated deciduous teeth (SHED). Cells were irradiated or not (control) with an InGaAlP laser diode (660  nm, 30 mW, continuous action mode) using two different energy densities (0.5 J/cm2—16 s; 1.0 J/cm2—33 s). Irradiation was performed at 0 and 48 h, with the laser probe fixed at a distance of 0.5 cm from the cells. Cell proliferation was analyzed at 0, 24, 48, and 72 h by the Trypan blue exclusion method and M...
Source: Lasers in Medical Science - October 12, 2017 Category: Laser Surgery Source Type: research

Biofilm formation by Candida albicans is inhibited by photodynamic antimicrobial chemotherapy (PACT), using chlorin e6: increase in both ROS production and membrane permeability
The objective of this study was to investigate the effects of PACT, using chlorin e6, as a photosensitizer onC. albicans. In this work, we studied the effect of PACT on both cell growth and biofilm formation byC. albicans. In addition, both ROS production and cell permeability were determined after PACT. PACT inhibited both growth and biofilm formation byC. albicans. We have also observed that PACT increased both ROS production (six times) and cell membrane permeability (five times) inC. albicans. PACT decreased both cell growth and biofilm development. The effect of PACT using chlorin e6 onC. albicans could be associated ...
Source: Lasers in Medical Science - October 9, 2017 Category: Laser Surgery Source Type: research

Efficacy of long pulse Nd:YAG laser versus fractional Er:YAG laser in the treatment of hand wrinkles
AbstractThere are different modalities for hand rejuvenation. Fractional Er:YAG laser and long pulse Nd:YAG laser were introduced for treating hand wrinkles. We plan to compare fractional Er:YAG laser and long pulse Nd:YAG laser in a randomized controlled double-blind design with multiple sessions and larger sample size in comparison with previous studies. Thirty-three participants with hand wrinkles entered this study. They were randomly allocated to undergo three monthly laser treatments on each hand, one with a fractional Er:YAG laser and the other with a long pulse Nd:YAG laser. The evaluations included assessment of c...
Source: Lasers in Medical Science - October 8, 2017 Category: Laser Surgery Source Type: research

A study on the clinical characteristics of treating nevus of Ota by Q-switched Nd:YAG laser
AbstractThe purpose of this study was to retrospectively analyze the clinical characteristics of treating nevus of Ota by Q-switched Nd:YAG laser in Laser Cosmetology Center of Department of Dermatology, the Second Hospital, Xi ’an Jiaotong University. The data of 1168 patients of nevus of Ota were analyzed retrospectively, which included the correlation among lesion color, treatment sessions, sex, age, lesion types, and effect. The Q-switched (QS) Nd:YAG laser system had a higher number of treatment sessions which were positively associated with a better response to treatment. Other variables, including gender, age,...
Source: Lasers in Medical Science - October 7, 2017 Category: Laser Surgery Source Type: research

Enhancement of photo-bactericidal effect of tetrasulfonated hydroxyaluminum phthalocyanine on Pseudomonas aeruginosa
AbstractAt the present time, photodynamic inactivation (PDI) is receiving considerable interest for its potential as an antimicrobial therapy. The results of our study indicate that enhancement of the phototoxic effect onPseudomonas aeruginosa can be achieved by combination of tetrasulfonated hydroxyaluminum phthalocyanine (AlPcS4) and bimetallic gold/silver nanoparticles (Au/Ag-NPs) synthesized by the cell-free filtrate ofAureobasidium pullulans. The bimetallic nanoparticles were characterized by a number of techniques including UV-vis, XPS, TEM, and SEM-EDS to be 14  ± 3 nm spherical particles coate...
Source: Lasers in Medical Science - October 6, 2017 Category: Laser Surgery Source Type: research

Low-power laser irradiation inhibits PDGF-BB-induced migration and proliferation via apoptotic cell death in vascular smooth muscle cells
AbstractVascular restenosis after injury of blood vessel has been implicated in various responses including apoptosis, migration, and proliferation in vascular smooth muscle cells (VSMCs) stimulated by diverse growth factors underlying platelet-derived growth factor (PDGF). Previous studies evaluated the effects of low-power laser (LPL) irradiation over various wavelength ranges on VSMC events in normal and pathologic states. However, whether VSMC responses are affected by LPL irradiation remains unclear. The purpose of this study is to explore the effects of LPL (green diode laser 532-nm pulsed wave of 300  mW at a s...
Source: Lasers in Medical Science - October 5, 2017 Category: Laser Surgery Source Type: research

Effects of photobiomodulation therapy and topical non-steroidal anti-inflammatory drug on skeletal muscle injury induced by contusion in rats —part 1: morphological and functional aspects
The objective of the present study was to evaluate and compare the effects of PBMT, with different application doses, and topical NSAIDs, under morphological and functional parameters, during an acute inflammatory process triggered by a controlled model of musculoskeletal injury induced via contusion in rats. Muscle injury was induced by means of a single trauma to the animals ’ anterior tibialis muscle. After 1 h, the rats were treated with PBMT (830 nm; continuous mode, with a power output of 100 mW; 3.57 W/cm2; 1  J–35.7 J/cm2, 3  J–107.1 J/cm2, and 9  J&nda...
Source: Lasers in Medical Science - October 5, 2017 Category: Laser Surgery Source Type: research

Development of a practical animal model of photodynamic therapy using a high concentration of extracellular talaporfin sodium in interstitial fluid: influence of albumin animal species on myocardial cell photocytotoxicity in vitro
AbstractPhotodynamic reaction-induced photocytotoxicity using talaporfin sodium is inhibited by serum proteins binding to talaporfin sodium. The serum albumin binding site for talaporfin sodium differs among animal species. To identify a practical animal therapeutic model, we studied the ability of human, canine, bovine, and porcine albumin to influence talaporfin sodium-induced photocytotoxicity in rat myocardial cells in vitro. Human, canine, bovine, and porcine serum albumins were used. The ratio of talaporfin sodium binding, which is strongly associated with photocytotoxicity, was measured by ultrafiltration with an al...
Source: Lasers in Medical Science - October 5, 2017 Category: Laser Surgery Source Type: research

Correction to: The changing landscape of dermatology practice: melanoma and pump-probe laser microscopy
AbstractThe published online version contains mistake. Warren S. Warren was not included in the author group section. Corrected author group section is shown above. (Source: Lasers in Medical Science)
Source: Lasers in Medical Science - October 5, 2017 Category: Laser Surgery Source Type: research

Treatment of striae distensae with non-ablative fractional laser: clinical and in vivo microscopic documentation of treatment efficacy
We report herein in vivo variations occurr ing in SD after NAFL treatment. (Source: Lasers in Medical Science)
Source: Lasers in Medical Science - October 4, 2017 Category: Laser Surgery Source Type: research

Clinical assessment of diode laser-assisted endoscopic intrasphenoidal vidian neurectomy in the treatment of refractory rhinitis
AbstractFor chronic rhinitis that is refractory to medical therapy, surgical intervention such as endoscopic vidian neurectomy (VN) can be used to control the intractable symptoms. Lasers can contribute to minimizing the invasiveness of ENT surgery. The aim of this retrospective study is to compare in patients who underwent diode laser-assisted versus traditional VN in terms of operative time, surgical field, quality of life, and postoperative complications. All patients had refractory rhinitis with a poor treatment response to a 6-month trial of corticosteroid nasal sprays and underwent endoscopic VN between November 2006...
Source: Lasers in Medical Science - October 3, 2017 Category: Laser Surgery Source Type: research

Laser-induced changes of in vitro erythrocyte sedimentation rate
AbstractThe study of the effects of low-level laser (LLL) radiation on blood is important for elucidating the mechanisms behind the interaction of LLL radiation and biologic tissues. Different therapy methods that involve blood irradiation have been developed and used for clinical purposes with beneficial effects. The aim of this study was to compare the effects of different irradiation protocols using a diode-pumped solid-state LLL (λ = 405 nm) on samples of human blood by measuring the erythrocyte sedimentation rate (ESR). Human blood samples were obtained through venipuncture into tubes containing ...
Source: Lasers in Medical Science - October 2, 2017 Category: Laser Surgery Source Type: research

Epigenetic changes in preimplantation embryos subjected to laser manipulation.
AbstractThe advantage of using laser for assisted hatching in routine assisted reproductive technology (ART) practice is debatable. Recently, it has been shown that laser-manipulated mouse embryos had compromised genetic integrity. However, the impact of laser-assisted hatching (LAH) on the epigenetic integrity of the preimplantation embryos is not elucidated so far. Since continuous thermal stress on embryos was found to lower mRNA levels of de novo (bovine) methyl transferases in embryos, we hypothesize that thermal energy induced during LAH may alter the epigenetic signature through abnormal de novo methyl transferases ...
Source: Lasers in Medical Science - September 30, 2017 Category: Laser Surgery Source Type: research