Effect of photobiomodulation on cellular migration and survival in diabetic and hypoxic diabetic wounded fibroblast cells
AbstractA disrupted wound repair process often leads to the development of chronic wounds, and pose a major physical, social and economic inconvenience on patients and the public health sector. Chronic wounds are a common complication seen in diabetes mellitus (DM), and often the severity necessitates amputation of the lower limbs. Recently, there has been increasing evidence that photobiomodulation (PBM) initiates wound healing, including increased protein transcription for cell proliferation, viability, migration and tissue reepithelialisation. Here, the hypothesis that PBM at a wavelength of 660  nm and energy dens...
Source: Lasers in Medical Science - June 1, 2020 Category: Laser Surgery Source Type: research

Photobiomodulation and salivary glands: a systematic review
AbstractTo date, there is no compilation of evidence-based information associating photobiomodulation effect and salivary glands. This systematic review aims to assess photobiomodulation effect of low intensity laser on salivary glands in the presence of systemic diseases. MEDLINE databases were searched in duplicate through December 2018. In vivo studies and clinical trials were included if photobiomodulation was performed in salivary glands of animal (rat or mice) or human in the presence of systemic disease. The methodological quality was assessed in duplicate using the modified Newcastle-Ottawa scale (NOS). Search stra...
Source: Lasers in Medical Science - May 29, 2020 Category: Laser Surgery Source Type: research

Effects of the combination of low-level laser therapy and anionic polymer membranes on bone repair
The objective of this study was to evaluate the regeneration of bone defects using collagen and elastin membranes derived from intestinal serosa and bovine auricular cartilage combined with low-level laser application. Thirty-six Wistar rats were operated to create a 3-mm defect in the distal metaphysis of the left femur and divided into six groups: G1 (control, no treatment); G2 (laser); G3 (elastin graft), G4 (elastin+laser); G5 (collagen graft); G6 (collagen+laser). The animals were sacrificed 6  weeks after surgery and the femurs were removed for analysis of bone repair. Macroscopic and radiological results showed...
Source: Lasers in Medical Science - May 29, 2020 Category: Laser Surgery Source Type: research

Could we diagnose childhood asthma by LIBS technique?
AbstractAsthma affects children related to trace elements. Our aim was to investigate the levels of trace elements using laser-induced breakdown spectroscopy (LIBS). The study included 120 children aged 4 –12 years from both sexes. They were divided into three groups: Group 1 of 40 children was diagnosed as cases of controlled asthma. Group 2 consisted of 40 children diagnosed as cases of uncontrolled asthma. Group 3 consisted of 40 children as normal control. Asthmatic children were classified ac cording to GINA 2015. They were analyzed for serum levels of total IgE, eosinophil count, and trace metals (Zn, Cu, ...
Source: Lasers in Medical Science - May 29, 2020 Category: Laser Surgery Source Type: research

Differential Mueller matrix imaging of partially depolarizing optically anisotropic biological tissues
AbstractSince recently, a number of innovative polarization-based optical imaging modalities have been introduced and extensively used in various biomedical applications, with an ultimate aim to attain the practical tool for the optical biopsy and functional characterization of biological tissues. The techniques utilize polarization properties of light and Mueller matrix mapping of microscopic images of histological sections of biological tissues or polycrystalline films of biological fluids. The main drawback of currently developed laser polarimetry approaches and Mueller matrix mapping techniques is poor reproducibility ...
Source: Lasers in Medical Science - May 29, 2020 Category: Laser Surgery Source Type: research

Split-face comparison of the picosecond 1064-nm Nd:YAG laser using a microlens array and the quasi-long-pulsed 1064-nm Nd:YAG laser for treatment of photoaging facial wrinkles and pores in Asians
AbstractSkin photoaging manifests deeper wrinkles and larger pore size. Various lasers have been tried for rejuvenation of photoaging skin, and the quasi-long-pulsed 1064-nm neodymium:yttrium-aluminum-garnet (Nd:YAG) laser is one promising treatment option. New types of laser device are emerging, including devices operating with picosecond pulse duration combined with a microlens array, which are regarded as a new breakthrough for skin rejuvenation. We aimed to evaluate the clinical effectiveness and safety of the picosecond 1064-nm Nd:YAG laser using a microlens array compared with the quasi-long-pulsed 1064-nm Nd:YAG las...
Source: Lasers in Medical Science - May 29, 2020 Category: Laser Surgery Source Type: research

Comparison of the effects of 10.6- μm infrared laser and traditional moxibustion in the treatment of knee osteoarthritis
The objective was to see whether the laser moxibustion is non-inferior to the traditional moxibustion in alleviating symptoms of knee osteoarthritis such as pain, stiffness, and joint dysfunction as well as improving quality of life for the patients with knee osteoarthritis, and whether a further RCT directly comparing the laser and traditional moxibustion is necessary. Poole d data from two RCTs in patients with knee osteoarthritis, trial ISRCTN68475405 and trial ISRCTN26065334, were used. In the two RCTs, the eligibility criteria were almost identical, the treatment procedure (i.e., sessions, duration, and points) were s...
Source: Lasers in Medical Science - May 29, 2020 Category: Laser Surgery Source Type: research

Photobiomodulation therapy is able to decrease IL1B gene expression in an in vitro cellular model of hidradenitis suppurativa
(Source: Lasers in Medical Science)
Source: Lasers in Medical Science - May 29, 2020 Category: Laser Surgery Source Type: research

In vitro anti-tumor effect of high-fluence low-power laser light on apoptosis of human colorectal cancer cells
AbstractColorectal cancer is the third most common malignancy all over the world, along with high morbidity and mortality. As a treatment, high-fluence low-power laser irradiation (HF-LPLI) has reported that its biostimulatory activity can suppress or even destruct tumor growth in neoplastic diseases. The aim of the present study is to examine a therapeutic capacity of HF-LPLI for colorectal cancer treatment by using human colon cancer cell (HT29) model. The in vitro cancer cell model was used to analyze the underlying mechanism of laser-induced apoptosis. Laser irradiation was performed five times (once a day for five con...
Source: Lasers in Medical Science - May 28, 2020 Category: Laser Surgery Source Type: research

Effectiveness of a combined 308-nm excimer lamp and topical mid-potent steroid treatment for facial vitiligo: a preliminary, randomized double-blinded controlled study
This study aimed to assess the effectiveness of a combined 308-nm excimer lamp and topical steroid therapy compared with the lamp alone for treating facial vitiligo. Symmetrical, nonsegmental facial vitiligo subjects were recruited. All facial lesions were irradiated with a 308-nm excimer lamp twice weekly for a total of 24 sessions. The lesions on each side of the face were randomly allocated to have topical mometasone furoate cream or the cream base alone applied once daily for 12  weeks. Clinical improvement was evaluated by a blinded dermatologist and the participants. A total of sixteen sides of the face were ass...
Source: Lasers in Medical Science - May 27, 2020 Category: Laser Surgery Source Type: research

Photobiomodulation with 808-nm diode laser enhances gingival wound healing by promoting migration of human gingival mesenchymal stem cells via ROS/JNK/NF- κB/MMP-1 pathway
AbstractPhotobiomodulation(PBM) has been shown to improve wound healing by promoting mesenchymal stem cell migration and proliferation. However, it remains unknown whether an 808-nm diode laser can influence human gingival mesenchymal stem cells (HGMSCs), and which dose this works well. In the present study, it was found that PBM could promote the migration of HGMSCs but not the proliferation. Furthermore, PBM could activate mitochondrial ROS, which could elevate the phosphorylation levels of JNK and IKB in HGMSCs, and further activate NF- κB as the nuclear translocation of p65 is elevated. Taken together, these pres...
Source: Lasers in Medical Science - May 26, 2020 Category: Laser Surgery Source Type: research

A comparative study of low-level laser efficacy on autologous activity of PRP injected in knee arthritis, in vivo study
The objectives of this study are to investigate the indirect and prolonged influence of laser irradiation (650  nm with 100 mW output power) on healing processes of knee joints with induced osteoarthritis (OA), by comparison of radiated and non-radiated PRP on repairing of joint cartilage. In material and methods, we used 9 rats divided in to four groups: C1, control without any treatment, for positive com parisons of healing; C2 and C3, controls with induced OA, left for 14 days, then sacrificed for histological analysis of negative comparisons; and P and L groups that had induced with OA for 14 days a...
Source: Lasers in Medical Science - May 21, 2020 Category: Laser Surgery Source Type: research

Evaluation of the effects of photobiomodulation therapy and ozone applications after gingivectomy and gingivoplasty on postoperative pain and patients ’ oral health-related quality of life
In this study, 36 patients with chronic inflammatory gingival enlargement underwent gingivectomy and gingivoplasty. The groups were randomly divided into control (n = 12), PBM (n = 12) and ozone (n = 12) groups. GaAlAs diode laser 810 nm wavelength at a non-contact and continuous mode with a power of 0.3 W and a density of 4 J/cm2 used for PBM for 1  min. Ozone was applied for 1 min for every 5 mm2 in contact mode at power level 9 using probe number 3. PBM and ozone applications were performed immediately after the operation, on the 3rd and 7th days. P...
Source: Lasers in Medical Science - May 20, 2020 Category: Laser Surgery Source Type: research

Laser treatment in retinitis pigmentosa-a review
AbstractRetinitis pigmentosa (RP) is a common inherited retinal disease for which effective treatment is not yet known. This review sought to analyze the available medical literature covering the efficacy of different forms of laser treatment for RP in laboratory and clinical trials. The PubMed database was searched using the following phrases: “laser photocoagulation”, “subthreshold laser”, “nanolaser”, “micropulse laser”, “retinitis pigmentosa”, “rod–cone dystrophy”, and “retinal dystrophy”. Results were stratified as clinical or ex...
Source: Lasers in Medical Science - May 20, 2020 Category: Laser Surgery Source Type: research

Mechanical transnasal endoscopic dacryocystorhinostomy versus transcanalicular multidiode laser dacryocystorhinostomy: long-term results of a prospective study
This study is a prospective randomized trial. Twenty-nine patients presenting persistent epiphora due to primary acquired nasolacrimal duct obstruction (PANDO) were included in the study. Two groups each consisting of 15 eyes were formed. Mechanical transnasal endoscopic DCR (MTE-DCR) was applied to the first group, while transcanalicular dacryocystorhinostomy with multidiode laser (TCML-DCR) techniques is employed in the second group. Follow-up is conducted in the first day, first week, and first month of the dacryocystorhinostomy which is followed by 4-month follow-up period, and results were compared using statistical m...
Source: Lasers in Medical Science - May 20, 2020 Category: Laser Surgery Source Type: research

Transoral laryngeal videosurgery under the direct guidance of narrow band imaging: a preliminary report
(Source: Lasers in Medical Science)
Source: Lasers in Medical Science - May 19, 2020 Category: Laser Surgery Source Type: research

Effects of photobiomodulation at different wavelengths on orthodontically induced root resorption in orthodontic retention period: a micro-CT and RT-PCR study
AbstractThe aim of this study was to evaluate and compare the reparative and inhibitory effects of single wavelength photobiomodulation (SW-PBM) and of cumulative increased wavelength photobiomodulation (CW-PBM) on orthodontically induced inflammatory root resorption (OIIRR). Thirty-three Wistar albino rats were divided into five groups: untreated group (negative control), only relapse group (positive control-1), only retention group (positive control-2), SW-PBM group (650  nm, 100 mW/cm2), and CW-PBM group (532 –650–940 nm, 100 mW/cm2). Orthodontic tooth movement was induced experimentally ...
Source: Lasers in Medical Science - May 12, 2020 Category: Laser Surgery Source Type: research

Laser enhancement of cancer cell destruction by photothermal therapy conjugated glutathione (GSH)-coated small-sized gold nanoparticles
AbstractThe current study presents the employment of glutathione (GSH)-modified small-sized gold nanoparticles (AuNPs) ~  3 nm in photothermal therapy (PTT), to evaluate the targeting and the toxic effect of cancer rather than normal cells. GSH is pH-sensitive surfaces that exhibit a fast response to the variation in pH conditions between normal (~ 7.4) and cancer cells (6–6.5). Results showed a considerable to xic impact via GSH-AuNP accumulation in cancer cells by both green and NIR laser irradiation. A proportional relation of cellular death to AuNP concentration, exposure time, and light-to-hea...
Source: Lasers in Medical Science - May 12, 2020 Category: Laser Surgery Source Type: research

Monitoring enamel caries on resin-treated occlusal surfaces using quantitative light-induced fluorescence: an in vitro study
AbstractThe aim of this study is to evaluate the ability of quantitative light-induced fluorescence (QLF) to monitor enamel caries lesions of different severity stages located on the occlusal surfaces of permanent teeth before and after treatment with resin infiltrant. Sixty extracted permanent teeth had one occlusal site selected and were categorized according to the International Caries Detection and Assessment System (ICDAS) criteria. The teeth were divided into three groups (n = 20): ICDAS 1, ICDAS 2, and ICDAS 3. The teeth were assessed by a trained examiner using QLF in two phases: (A) before and (B) af...
Source: Lasers in Medical Science - May 7, 2020 Category: Laser Surgery Source Type: research

Influence of biosafety materials of the laser output power
AbstractBiosafety materials used in the correct handling of low power laser equipment may interfere on the power delivered at the target tissue and, possibly, on the effects on biological tissues. The aim of this study was to evaluate the interference of the use of polyvinyl chloride (PVC) and polyethylene (PEAD) protection materials on the output power of low power lasers. Two low power diode laser devices with different wavelengths (red and infrared) were used. For each wavelength, two protection materials and two evaluation times (before and after protection) were considered. The output power (mW) was measured with the ...
Source: Lasers in Medical Science - May 6, 2020 Category: Laser Surgery Source Type: research

Laser fabrication of structural bone: surface morphology and biomineralization assessment
AbstractThe current work explores the surface morphology of the laser-ablated bone using Yb-fiber coupled Nd:YAG laser (λ = 1064 nm) in continuous wave mode. As the laser-ablated region contains physiochemically modified carbonized and nonstructural region, it becomes unknown material for the body. Thus, biomineralization on such a laser-ablated region was assessed by in vitro immersion test in noncellular simulated body fluid. The presence of hydroxyapatite was detected in the precipitated mineral product using scanning electron microscopy equipped with energy dispersive spectroscopy, and X-ray diffraction analysis...
Source: Lasers in Medical Science - May 6, 2020 Category: Laser Surgery Source Type: research

Nonlinear optical microscopy is a novel tool for the analysis of cutaneous alterations in pseudoxanthoma elasticum
AbstractPseudoxanthoma elasticum (PXE, OMIM 264800) is a rare autosomal recessive disorder with ectopic mineralization and fragmentation of elastin fibers. It is caused by mutations of theABCC6 gene that leads to decreased serum levels of inorganic pyrophosphate (PPi) anti-mineralization factor. The occurrence of severe complications among PXE patients highlights the importance of early diagnosis so that prompt multidisciplinary care can be provided to patients. We aimed to examine dermal connective tissue with nonlinear optical (NLO) techniques, as collagen emits second-harmonic generation (SHG) signal, while elastin can ...
Source: Lasers in Medical Science - May 6, 2020 Category: Laser Surgery Source Type: research

Conditioned media from blue light-emitting diode –exposed fibroblasts have an anti-inflammatory effect in vitro
AbstractWe have previously reported the protective effects of blue light-emitting diode (BLED) –stimulated cell metabolites on cell injury. To further examine the effect of conditioned media (CM) derived from BLED (5 J/cm2)-exposed human normal fibroblasts (CMBL5) for clinical application, we have used the choline chloride and phenol red –free media and then concentrated CMBL5 using a centrifugal filter unit. The collected CMBL5-lower part (CMBL5-LO) has evaluated the inflammatory protein expression profile in LPS-stimulated RAW264.7 cells. Comprehensive metabolomic profiling of CMBL5-LO was carried out us...
Source: Lasers in Medical Science - May 4, 2020 Category: Laser Surgery Source Type: research

Management of cystoid macular edema secondary to retinitis pigmentosa via subliminal micropulse yellow laser
AbstractTo investigate the effects of subliminal micropulse yellow laser application on the central macular thickness and best-corrected visual acuity in cystoid macular edema secondary to retinitis pigmentosa patients. This prospective open-label clinical trial, conducted between January 2018 and October 2019, included 32 eyes of 29 patients who had cystoid macular edema secondary to retinitis pigmentosa. Patients were treated by subliminal micropulse yellow laser for one session. Central macular thickness and best-corrected visual acuity changes were investigated just before the treatment and 1  year later after the...
Source: Lasers in Medical Science - May 4, 2020 Category: Laser Surgery Source Type: research

Intraoperative use of optical coherence tomography to differentiate normal and diseased thyroid and parathyroid tissues from lymph node and fat
This study is the first in vivo OCT imaging study to evaluate bo th normal and diseased tissues that may be encountered during neck surgery. OCT has the potential to become a valuable intraoperative tool to differentiate diseased and normal thyroid tissue intraoperatively to obtain an “optical biopsy” in real time without fixation, staining, or tissue resecti on. (Source: Lasers in Medical Science)
Source: Lasers in Medical Science - April 27, 2020 Category: Laser Surgery Source Type: research

Sensory and motor responses after photobiomodulation associated with physiotherapy in patients with incomplete spinal cord injury: clinical, randomized trial
AbstractComplete or incomplete spinal cord injury (SCI) results in permanent neurological deficits due to the interruption of nerve impulses, causing the loss of motor and sensory function, which leads to a reduction in quality of life. The focus of rehabilitation for such individuals is to improve quality of life and promote functional recovery. Photobiomodulation (PBM) has proved to be promising complementary treatment in cases of SCI. The aim of the present study was to investigate the effects of PBM combined with physiotherapy on sensory-motor responses below the level of the injury and quality of life in individuals w...
Source: Lasers in Medical Science - April 26, 2020 Category: Laser Surgery Source Type: research

Identification of oral cancer in OCT images based on an optical attenuation model
AbstractSurgery is still the first choice to treat oral cancer, where it is important to detect surgical margins in order to reduce cancer recurrence and maintain oral-maxillofacial function simultaneously. As a non-invasive and in situ imaging technique, optical coherence tomography (OCT) can obtain images close to the resolution of histopathology, which makes it have great potential in intraoperative diagnosis. However, it is not enough to find surgical margins accurately just observing OCT images directly and qualitatively. The purpose of this study is to identify oral cancer in OCT images by investigating the quantitat...
Source: Lasers in Medical Science - April 25, 2020 Category: Laser Surgery Source Type: research

The effects of laser stimulation at acupoint ST36 on anxiety-like behaviors and anterior cingulate cortex c-Fos expression in a rat post-traumatic stress disorder model
This study was designed to assess how these behaviors and anterior cingulate cortex (ACC) c-Fos expression were impacted by 10.6- μm laser stimulation at acupoint ST36 a rat model of PTSD. A rat model of PTSD was prepared via prolonged exposure of animals to a stressor, followed by a 7-day period during which animals were allowed to rest undisturbed in their cages. Rats were randomized into four experimental groups (n = 12/group): the control, PTSD, LS, and sham LS groups. Control group animals were not subjected to SPS procedures prior to behavioral testing. LS and sham LS animals were administered LS tre...
Source: Lasers in Medical Science - April 24, 2020 Category: Laser Surgery Source Type: research

Clinical performance of clinical-visual examination, digital bitewing radiography, laser fluorescence, and near-infrared light transillumination for detection of non-cavitated proximal enamel and dentin caries
AbstractThe aim of this study was to compare the clinical performance of clinical-visual examination using the International Caries Detection and Assessment System (ICDAS) II, digital bitewing radiography, near-infrared light transillumination (NIR-LT), and laser fluorescence (LF) for the detection of non-cavitated proximal enamel and dentin caries. The study included 335 patients, aged 12 –18 years, with no cavities in the posterior teeth. Clinical-visual inspections of 335 non-cavitated proximal caries were performed by two examiners. For enamel caries, clinical validation included a combination of clinical-vi...
Source: Lasers in Medical Science - April 24, 2020 Category: Laser Surgery Source Type: research

Photobiomodulation therapy increases functional capacity of patients with chronic kidney failure: randomized controlled trial
AbstractPhotobiomodulation (PBM) has been used in different populations as a strategy to attenuate muscle fatigue and improve exercise performance. Recent findings demonstrated that a single session with specific PBM doses during hemodialysis (HD) increased the upper limb muscle strength of chronic kidney failure (CKF) patients. Now, the primary objective of this study was to evaluate the chronic effect of PBM on the functional capacity of this population. Secondarily, we aimed at investigating the effects of PBM on the patients ’ strength, muscle thickness and echogenicity, perception of pain, fatigue, and quality o...
Source: Lasers in Medical Science - April 24, 2020 Category: Laser Surgery Source Type: research

Application of laser for treatment of cutaneous leishmaniasis: a review of literature
AbstractCutaneous leishmaniasis (CL) is a major health problem in developing countries with high economic and health impact. Despite suggested treatment for CL, there is still no definite therapy for this infection, and many of these treatments are associated with serious local and systemic side effects. In the current paper, use of different laser types including continuous and fractional CO2, argon, PDL, erbium glass, and Nd:YAG have been reviewed. Based on our review, given the high reported efficacy and low side-effect profile, use of laser can be considered as a good alternative to standard treatment of cutaneous leis...
Source: Lasers in Medical Science - April 24, 2020 Category: Laser Surgery Source Type: research

Efficacy of low-level laser therapy on management of Bell ’s palsy: a systematic review
AbstractThe aim of this study is to evaluate the efficacy of low-level laser therapy (LLLT) in patients with Bell ’s palsy (BP) through a systematic review method. We systematically searched international databases including PubMed, Scopus, and Web of Science to find eligible articles without language limitation. All relevant randomized controlled trials (RCTs) that compared the efficacy of the LLLT with plac ebo laser, exercise, massage, or no intervention on BP patients were included. Four studies (out of 259) had met our inclusion criteria involving 171 patients and were entered to the systematic review. Full text...
Source: Lasers in Medical Science - April 21, 2020 Category: Laser Surgery Source Type: research

Excimer laser coronary atherectomy prior to paclitaxel-coated balloon angioplasty for de novo coronary artery lesions
This study aimed to evaluate the efficacy and safety of excimer laser coronary atherectomy (ELCA) prior to paclitaxel-coated balloon angioplasty for de novo coronary artery lesions. This retrospective observational study analyzed 118 eligible patients with de novo coronary artery disease whose only percutaneous coronary intervention was a drug-coated balloon angioplasty (i.e., no subsequent stent placement). Data related to our primary outcomes of interest —incidence of major adverse cardiovascular and cerebral events (MACCE), and incidence of procedural complications (bailout stenting and minor complications)—...
Source: Lasers in Medical Science - April 18, 2020 Category: Laser Surgery Source Type: research

Evaluation of adjunctive efficacy of diode laser in the treatment of peri-implant mucositis: a randomized clinical trial
AbstractPeri-implant disease may affect survival of dental implants. The aim of the study is to analyze the effectiveness of diode laser as a supportive modality to the non-surgical conventional treatment of peri-implant mucositis (PiM) and initial peri-implantitis (PI). Twenty-three patients with single implants suffering from PiM or initial PI were selected and randomly divided into two groups; control group (CG) received non-surgical conventional treatment, and test group (TG) received non-surgical conventional treatment and diode laser application with wavelength of 980  nm. Probing pocket depth (PPD) and bleeding...
Source: Lasers in Medical Science - April 18, 2020 Category: Laser Surgery Source Type: research

Outcome of a 980-nm diode laser coagulation in women with radiation-induced hemorrhagic cystitis: a single-center retrospective study
AbstractThe purpose of this study was to evaluate the efficacy of a 980-nm diode laser coagulation in women with radiation-induced hemorrhagic cystitis (RHC). We conducted a retrospective study of 21 RHC patients treated with a 980-nm diode laser between July 2014 and December 2017 at our institution. Data was collected with regard to age, sex, lower urinary tract symptoms, use of transfusions, a drop in hemoglobin levels, indication of radiotherapy, median time between radiation therapy and presentation, previous treatments, operative time, mean energy used, number of coagulated areas, catheterization time, discharge time...
Source: Lasers in Medical Science - April 18, 2020 Category: Laser Surgery Source Type: research

Photobiomodulation alters the viability of HUVECs cells
AbstractThe aim of the present study was to investigate the influence of low-level red (660  nm) and infrared (780 nm) laser with four different radiance exposures on human umbilical vein endothelial cells (HUVECs) in vitro. HUVECs (1.5  × 104) were incubated in 96-well culture plates. The cells were maintained in M199 medium supplemented with 20% fetal bovine serum, 1% antibiotic (penicillin), 1% anti-mycotic (Fungizone), and 1% endothelial cell growth supplement. After centrifugation, irradiations (660/780  nm, 40 mW, 1, 5, 10, and 20 J/cm2, 1  s, 5 s, 10 s, and...
Source: Lasers in Medical Science - April 17, 2020 Category: Laser Surgery Source Type: research

Clinical improvement of photoaging-associated facial hyperpigmentation in Korean skin with a picosecond 1064-nm neodymium-doped yttrium aluminum garnet laser
This study therefore evaluated the efficacy and safety of picosecond 1064-nm Nd:YAG laser application for photoaging-associated facial pigmentation treatment in Korean subjects. Forty-seven Korean subjects with photoaging-associated facial pigmentation underwent picosecond 1064-nm laser application. The clinical improvement of 17 patients was assessed by objective measurements such as melanin and erythema indices. All subjects received six biweekly treatments with the laser in a three-pass fashion delivering approximately 2000 to 2500 shots using a zoom handpiece with a spot size of 7  mm, fluence ranging from 0.4 to ...
Source: Lasers in Medical Science - April 17, 2020 Category: Laser Surgery Source Type: research

Wavefront-guided photorefractive keratectomy with and without iris registration: comparison of astigmatic correction
This study aimed to compare the visual outcomes after photorefractive keratectomy (PRK), with and without the iris registration (IR) technology. The retrospective cohort study was performed for wavefront-guided PRK using the Technolas 217z100 excimer laser system in patients with myopic astigmatism (cylinder error − 2 to − 4 diopter [D]). Eyes were divided into IR group (IRPRK) and non-IR group (non-IRPRK). Visual acuity (VA), cylindrical refraction, and the astigmatic vector components using the Alpins method were compared between the two groups preoperatively and 6 months postoperatively. F...
Source: Lasers in Medical Science - April 15, 2020 Category: Laser Surgery Source Type: research

Exact location of sensorimotor cortex injury after photochemical modulation; evidence of stroke based on stereological and morphometric studies in mice
AbstractThe integrity of the structural cerebral cortex is disrupted after stroke either at the macroscopic or microscopic levels. Therefore, many attempts have been gathered to circumvent stroke-associated problems in the brain tissue. The current study was aimed to design an animal model of photochemical stroke using rose bengal (RB) plus laser irradiation (L) after 10, 15, and 20  min (´) and evaluate its effect on the cerebral tissue using unbiased stereological quantitative methods and morphometric histological analysis. Photochemical stroke was induced by intraperitoneal injection of RB dye and further act...
Source: Lasers in Medical Science - April 15, 2020 Category: Laser Surgery Source Type: research

Development of a new system to collect and dry patient exhalation samples for laser photo-acoustic gas analyzer
AbstractThe present work is devoted to the development of a new system for collecting and drying patient exhalation samples for a laser photo-acoustic gas analyzer for express analysis of exhaled air, as well as the possibility of ozone sterilization of the gas tract after use. It is proposed to collect the patient ’s exhalation in a disposable sterile plastic bag, place it in a low-temperature freezer chamber to freeze the water vapor, and then transfer part of the dried exhaled sample to the gas analyzer using a disposable syringe. It is proposed to use ozone purge for 10–15 min to sterilize the gas pat ...
Source: Lasers in Medical Science - April 14, 2020 Category: Laser Surgery Source Type: research

Rapid screening of hepatitis B using Raman spectroscopy and long short-term memory neural network
This study presents a rapid method to screen hepatitis B patients using serum Raman spectroscopy combined with long short-term memory neural network (LSTM). The serum samples taken from 435 hepatitis B patients and 699 non-hepatitis B people were measured in this experiment. Specific biomolecular changes in three groups of serum samples could be seen in the tentative assignment of Raman peaks. First, principal component analysis (PCA) was used for extracting key features of spectral data, which reduces the dimension of the multidimensional spectrum. Then, LSTM is used to train the spectral data. Finally, the full connectio...
Source: Lasers in Medical Science - April 13, 2020 Category: Laser Surgery Source Type: research

Thermal coagulum formation and hemostasis during repeated multipulse Nd:YAG laser treatment of cutaneous vascular lesions: animal experiment study
In this study, repeated multipulse laser (RMPL) irradiation was proposed to induce acute thermal damage to target blood vessels with low incident fluence (40  J/cm2 for 1064-nm Nd:YAG laser). The feasibility of the method was investigated using animal models. Repeated multipulse irradiation cycles with 10-min intervals were performed in RMPL. A hamster dorsal skin chamber model with a visualization system was constructed to investigate the instant generation of thermal coagulum and relevant hemostasis by thrombus formation during and after irradiation under 1064  nm Nd:YAG single multipulse laser (SMPL)...
Source: Lasers in Medical Science - April 10, 2020 Category: Laser Surgery Source Type: research

Numerical reconstruction of turbid slab optical properties using global optimization algorithms
AbstractThe detection and reconstruction of the optical properties within turbid slabs/plate parallel mediums have been widely investigated for its applications in medical diagnosis, atmosphere detection, etc., where the scattering of light would be expected. Although the scattering signal can be utilized for diagnostics purposes, the multiple scattering in the intermediate scattering regime (with an optical depth ~  2–9) has posed a remarkable challenge. Existing optical tomography methods usually only reconstruct the reduced scattering coefficient to investigate the properties of the scattering target, while...
Source: Lasers in Medical Science - April 10, 2020 Category: Laser Surgery Source Type: research

Retrospective study of the treatment of port-wine stains with 595-nm pulsed dye laser in 261 Chinese patients
In this study, the efficacy of 595-nm PDL in treatment of PWSs in Chinese patients was investigated. A follow-up visit was performed on 261 Fitzpatrick skin type II–III Chinese patients with PWS who received more than two trea tments with 595-nm PDL. Based on cosmetic improvement, treatment response was graded as follows: complete, if color blanching was>  90%; significant, if color blanching was 60–90%; moderate, if color blanching was 30–60%; and poor, if color blanching was
Source: Lasers in Medical Science - April 10, 2020 Category: Laser Surgery Source Type: research

Combined pulsed dye laser and systemic retinoids for the treatment of hypertrophic resistant warts among organ transplant patients
(Source: Lasers in Medical Science)
Source: Lasers in Medical Science - April 7, 2020 Category: Laser Surgery Source Type: research

Low-level laser therapy for weight reduction: a randomized pilot study
AbstractObesity and overweight is a global health crisis and novel methods of treatment are needed to address it. Low-level laser therapy (LLLT) is a currently available non-invasive procedure for lysing excess fat, but there is a lack of consensus exists on LLLT frequency and limited research from studies of LLLT. The purpose of this pilot study is to compare the effect of three of the most common LLLT frequencies on weight, waist circumference, body fat percentage, and quality of life. Sixty overweight (body mass index (BMI) 25 –29.9 kg/m2) adult participants were randomized to 12 LLLT treatments: (1) three ti...
Source: Lasers in Medical Science - March 23, 2020 Category: Laser Surgery Source Type: research

Evaluation of metalloproteinases-2, -9, and -13 post photobiomodulation in mice talocrural joint
AbstractThe extracellular matrix (ECM) is the main constituent of connective tissue with structural and regulatory functions, stimulating cell differentiation and proliferation. Moreover, ECM is a dynamic structure in the constant remodeling process, which is controlled by a balance between metalloproteinases (MMPs) and their inhibitors (TIMPs). Photobiomodulation (PBM) is widely described in the literature and applied in clinical practices, although its effects on ECM have not yet been elucidated. Therefore, it was evaluated if PBM could alter ECM components, such as MMP-2, -9, -13, and TIMP-2 from mice talocrural joints....
Source: Lasers in Medical Science - March 19, 2020 Category: Laser Surgery Source Type: research

In vitro anti-tumor effect of low-power laser irradiation (LPLI) on gastroenterological carcinoma cells
AbstractPrimary hepatocellular carcinoma (HCC) and colon carcinoma are two of the most common clinical malignancies along with high morbidity and mortality. As low-power laser irradiation (LPLI) can induce cytotoxicity or cell apoptosis on several types of hyperplasia, LPLI may be a potential alternative treatment for gastroenterological cancers. The current in vitro study focused on LPLI-induced apoptosis and mechanism after 532-nm laser irradiation on two different carcinoma cells. Squamous cell carcinoma (VX2) and murine colon carcinoma (CT26) cells were cultured to test the feasibility of LPLI. The applied fluence vari...
Source: Lasers in Medical Science - March 10, 2020 Category: Laser Surgery Source Type: research

Photobiomodulation effect on local hemoglobin concentration assessed by near-infrared spectroscopy in humans
AbstractExposure of biological tissue to photobiomodulation therapy (PBMT) seems to increase the oxygen availability and mitochondrial electrochemical activity. With the advancement of new technologies, such as near-infrared spectroscopy (NIRS), information can be obtained about the balance between oxygen utilization and delivery by assessing local oxy- ([O2Hb]) and deoxy-myohemoglobin ([HHb]) concentrations, both measured in micromolars ( μM). Consequently, NIRS can be used to study (“in vivo”) PBMT effects on the oxidative system, including oxygen availability. Thus, the main objective of the present study...
Source: Lasers in Medical Science - March 10, 2020 Category: Laser Surgery Source Type: research

Changes in corneal biomechanics during small-incision lenticule extraction (SMILE) and femtosecond-assisted laser in situ keratomileusis (FS-LASIK)
AbstractObserve the influence of femtosecond laser cutting on corneal biomechanics during small-incision lenticule extraction (SMILE) or femtosecond-assisted laser in situ keratomileusis (FS-LASIK) and assess the biomechanical changes following the operation. Prospective, non-randomized study. A total of 80 eyes from 80 patients were treated with either SMILE or FS-LASIK. Parameters of inverse concave radius, deformation amplitude ratio 2  mm or 1 mm (DA ratio 2 mm or 1 mm), highest concavity radius (HC radius), biomechanically corrected intraocular pressure (bIOP), and central corneal thickness (CCT) m...
Source: Lasers in Medical Science - March 10, 2020 Category: Laser Surgery Source Type: research