Protective effects of N-acetylcysteine and S-adenosyl-Lmethionine against nephrotoxicity and immunotoxicity induced by ochratoxin A in rats
CONCLUSION: NAC and SAMe have a protective role against nephrotoxicity and immunotoxicity induced by continuous administration of OTA. NAC was more effective in reducing OTA nephrotoxicity, whereas SAMe was more potent than NAC in reducing OTA immunotoxicity.PMID:38455596 | PMC:PMC10915916 (Source: Cancer Control)
Source: Cancer Control - March 8, 2024 Category: Cancer & Oncology Authors: Nuha A Alkhattabi Fares K Khalifa Huda A Al Doghaither Ayat B Al-Ghafari Nesrin Ibrahim Tarbiah Aliaa Sabban Source Type: research
Pioglitazone, a peroxisome proliferator ‑activated receptor γ agonist, induces cell death and inhibits the proliferation of hypoxic HepG2 cells by promoting excessive production of reactive oxygen species
Oncol Lett. 2024 Feb 19;27(4):160. doi: 10.3892/ol.2024.14294. eCollection 2024 Apr.ABSTRACTHypoxia is a hallmark of solid tumors. Hypoxic cancer cells adjust their metabolic characteristics to regulate the production of cellular reactive oxygen species (ROS) and facilitate ROS-mediated metastasis. Peroxisome proliferator-activated receptor γ (PPARγ) is a nuclear receptor that regulates the transcription of fatty acid metabolism-related genes that have a key role in the survival and proliferation function of hypoxic cancer cells. In the present study, mRNA expression in HepG2 cells under chemically induced hypoxia was as...
Source: Oncology Letters - March 7, 2024 Category: Cancer & Oncology Authors: Guohao Huang Mengfan Zhang Manzhou Wang Wenze Xu Xuhua Duan Xinwei Han Jianzhuang Ren Source Type: research
LC-MS/MS determination of N-acetyl-l-cysteine in chicken plasma
Biomed Chromatogr. 2024 Mar 3:e5854. doi: 10.1002/bmc.5854. Online ahead of print.ABSTRACTN-acetyl-l-cysteine (NAC) shows beneficial effects in cases of aflatoxicosis and heat stress in poultry but little is known about its pharmacokinetics in chickens. Therefore, the study aimed to develop and validate a sensitive LC-MS/MS analytical method for quantitative analysis of NAC in chicken plasma. A split calibration curve approach was used for determination of NAC in chicken plasma. Standard curves for low (0.05-2.5 μg/ml) and high (2.5-100 μg/ml) ranges of concentrations were prepared. The standard curves for low (r2 = 0.99...
Source: Biomedical Chromatography : BMC - March 3, 2024 Category: Biomedical Science Authors: Albena Roydeva Aneliya Milanova Source Type: research
LC-MS/MS determination of N-acetyl-l-cysteine in chicken plasma
Biomed Chromatogr. 2024 Mar 3:e5854. doi: 10.1002/bmc.5854. Online ahead of print.ABSTRACTN-acetyl-l-cysteine (NAC) shows beneficial effects in cases of aflatoxicosis and heat stress in poultry but little is known about its pharmacokinetics in chickens. Therefore, the study aimed to develop and validate a sensitive LC-MS/MS analytical method for quantitative analysis of NAC in chicken plasma. A split calibration curve approach was used for determination of NAC in chicken plasma. Standard curves for low (0.05-2.5 μg/ml) and high (2.5-100 μg/ml) ranges of concentrations were prepared. The standard curves for low (r2 = 0.99...
Source: Biomedical Chromatography : BMC - March 3, 2024 Category: Biomedical Science Authors: Albena Roydeva Aneliya Milanova Source Type: research
LC-MS/MS determination of N-acetyl-l-cysteine in chicken plasma
Biomed Chromatogr. 2024 Mar 3:e5854. doi: 10.1002/bmc.5854. Online ahead of print.ABSTRACTN-acetyl-l-cysteine (NAC) shows beneficial effects in cases of aflatoxicosis and heat stress in poultry but little is known about its pharmacokinetics in chickens. Therefore, the study aimed to develop and validate a sensitive LC-MS/MS analytical method for quantitative analysis of NAC in chicken plasma. A split calibration curve approach was used for determination of NAC in chicken plasma. Standard curves for low (0.05-2.5 μg/ml) and high (2.5-100 μg/ml) ranges of concentrations were prepared. The standard curves for low (r2 = 0.99...
Source: Biomedical Chromatography : BMC - March 3, 2024 Category: Biomedical Science Authors: Albena Roydeva Aneliya Milanova Source Type: research
Prolyl hydroxylase inhibition protects against murine MC903-induced skin inflammation by downregulating TSLP
In this study, we investigated the protective effect of DMOG on MC903 (calcipotriol)-induced type 2 skin inflammation. Morphological and immunological changes were assessed by H-E staining, flow cytometry and RT-qPCR. DMOG treatment attenuated MC903-induced skin inflammation in a T cell-independent manner. The anti-inflammatory effect of DMOG was accompanied by downregulation of TSLP and IL-33, and supplementation with recombinant TSLP and IL-33 abolished the effect of DMOG. MC903 increased ROS levels in skin tissue, which was prevented by DMOG. Furthermore, the ROS scavenger N-acetylcysteine (NAC) downregulated TSLP and a...
Source: Frontiers in Immunology - March 1, 2024 Category: Allergy & Immunology Source Type: research
Excavatolide C/cisplatin combination induces antiproliferation and drives apoptosis and DNA damage in bladder cancer cells
Arch Toxicol. 2024 Feb 29. doi: 10.1007/s00204-024-03699-1. Online ahead of print.ABSTRACTExcavatolide C (EXCC), a marine coral-derived compound, exhibits an antiproliferation effect on bladder cancer cells. The present study evaluated the improvement in the antiproliferation ability of EXCC by co-treatment with cisplatin in bladder cancer cells. EXCC/cisplatin (12.5 and 1 μg/mL) showed higher antiproliferation effects on bladder cancer cells than single treatments (EXCC or cisplatin alone) in the 48 h ATP assay. EXCC/cisplatin also enhanced the increase in subG1, annexin V-mediated apoptosis, and activation of poly (ADP-...
Source: Archives of Toxicology - February 29, 2024 Category: Toxicology Authors: Tsu-Ming Chien Che-Wei Yang Chia-Hung Yen Bi-Wen Yeh Wen-Jeng Wu Jyh-Horng Sheu Hsueh-Wei Chang Source Type: research
Mechanical overload-induced release of extracellular mitochondrial particles from tendon cells leads to inflammation in tendinopathy
In this study, we identified for the first time that tendon cells can release extracellular mitochondria (ExtraMito) particles, a subtype of medium extracellular particles (mEPs), into the environment through a process regulated by mechanical loading. RNA sequencing systematically revealed that oxygen-related reactions, extracellular particles, and inflammation were present in diseased human tendons, suggesting that these factors play a role in the pathogenesis of tendinopathy. We simulated the disease condition by imposing a 9% strain overload on three-dimensional mouse tendon constructs in our cyclic uniaxial stretching ...
Source: Molecular Medicine - February 29, 2024 Category: Molecular Biology Authors: Ziming Chen Mengyuan Li Peilin Chen Andrew Tai Jiayue Li Euphemie Landao Bassonga Junjie Gao Delin Liu David Wood Brendan F Kennedy Qiujian Zheng Ming H Zheng Source Type: research
Excavatolide C/cisplatin combination induces antiproliferation and drives apoptosis and DNA damage in bladder cancer cells
Arch Toxicol. 2024 Feb 29. doi: 10.1007/s00204-024-03699-1. Online ahead of print.ABSTRACTExcavatolide C (EXCC), a marine coral-derived compound, exhibits an antiproliferation effect on bladder cancer cells. The present study evaluated the improvement in the antiproliferation ability of EXCC by co-treatment with cisplatin in bladder cancer cells. EXCC/cisplatin (12.5 and 1 μg/mL) showed higher antiproliferation effects on bladder cancer cells than single treatments (EXCC or cisplatin alone) in the 48 h ATP assay. EXCC/cisplatin also enhanced the increase in subG1, annexin V-mediated apoptosis, and activation of poly (ADP-...
Source: Archives of Toxicology - February 29, 2024 Category: Toxicology Authors: Tsu-Ming Chien Che-Wei Yang Chia-Hung Yen Bi-Wen Yeh Wen-Jeng Wu Jyh-Horng Sheu Hsueh-Wei Chang Source Type: research
LCP1-mediated cytoskeleton alterations involve in arsenite-triggered malignant phenotype of human immortalized prostate stromal cells
Food Chem Toxicol. 2024 Feb 26:114548. doi: 10.1016/j.fct.2024.114548. Online ahead of print.ABSTRACTThe connection between continuous arsenic exposure and prostate cancer is already established. However, the exact mechanisms of arsenic tumorigenesis are far from clear. Here, we employed human prostate stromal immortalized cells (WPMY-1) continuous exposure to 1 and 2 μM arsenite for 29 weeks to identify the malignant phenotype and explore the underlying molecular mechanism. As expected, continuous low-dose arsenite exposure led to the malignant phenotype of WPMY-1 cells. Quantitative proteomics identified 517 differentia...
Source: Food and Chemical Toxicology - February 28, 2024 Category: Food Science Authors: Yiping Yang Menghan Zhou Yurun Huang Xiaotong Ye Yingxi Mo Yi Huang Shan Wang Source Type: research
Organoids as a biomarker for personalized treatment in metastatic colorectal cancer: drug screen optimization and correlation with patient response
CONCLUSIONS: Our study emphasizes the critical impact of the screening methods for determining correlation between PDO drug screens and mCRC patient outcomes. Our 5-step optimization strategy provides a basis for future research on the clinical utility of PDO screens.PMID:38414064 | DOI:10.1186/s13046-024-02980-6 (Source: Clinical Colorectal Cancer)
Source: Clinical Colorectal Cancer - February 28, 2024 Category: Cancer & Oncology Authors: Lidwien P Smabers Emerens Wensink Carla S Verissimo Esmee Koedoot Katerina-Chara Pitsa Maarten A Huismans Celia Higuera Bar ón Mayke Doorn Liselot B Valkenburg-van Iersel Geert A Cirkel Anneta Brousali Ren é Overmeer Miriam Koopman Manon N Braat Bas Pen Source Type: research
LCP1-mediated cytoskeleton alterations involve in arsenite-triggered malignant phenotype of human immortalized prostate stromal cells
Food Chem Toxicol. 2024 Feb 26:114548. doi: 10.1016/j.fct.2024.114548. Online ahead of print.ABSTRACTThe connection between continuous arsenic exposure and prostate cancer is already established. However, the exact mechanisms of arsenic tumorigenesis are far from clear. Here, we employed human prostate stromal immortalized cells (WPMY-1) continuous exposure to 1 and 2 μM arsenite for 29 weeks to identify the malignant phenotype and explore the underlying molecular mechanism. As expected, continuous low-dose arsenite exposure led to the malignant phenotype of WPMY-1 cells. Quantitative proteomics identified 517 differentia...
Source: Food and Chemical Toxicology - February 28, 2024 Category: Food Science Authors: Yiping Yang Menghan Zhou Yurun Huang Xiaotong Ye Yingxi Mo Yi Huang Shan Wang Source Type: research
LCP1-mediated cytoskeleton alterations involve in arsenite-triggered malignant phenotype of human immortalized prostate stromal cells
Food Chem Toxicol. 2024 Feb 28;186:114548. doi: 10.1016/j.fct.2024.114548. Online ahead of print.ABSTRACTThe connection between continuous arsenic exposure and prostate cancer is already established. However, the exact mechanisms of arsenic tumorigenesis are far from clear. Here, we employed human prostate stromal immortalized cells (WPMY-1) continuous exposure to 1 and 2 μM arsenite for 29 weeks to identify the malignant phenotype and explore the underlying molecular mechanism. As expected, continuous low-dose arsenite exposure led to the malignant phenotype of WPMY-1 cells. Quantitative proteomics identified 517 differe...
Source: Food and Chemical Toxicology - February 28, 2024 Category: Food Science Authors: Yiping Yang Menghan Zhou Yurun Huang Xiaotong Ye Yingxi Mo Yi Huang Shan Wang Source Type: research
Isolation and Bioactivity Evaluation of Sesquiterpenes from an Alcyonarian of the Genus Lemnalia from the Saudi Arabian Red Sea
Chem Biodivers. 2024 Feb 27:e202400235. doi: 10.1002/cbdv.202400235. Online ahead of print.ABSTRACTOver the last decades, soft corals have been proven a rich source of biologically active compounds, featuring a wide range of chemical structures. Herein, we investigated the chemistry of an alcyonarian of the genus Lemnalia (Neptheidae), specimens of which were collected from the coral reefs near Al Lith, on the south-west coast of Saudi Arabia. A series of chromatographic separations led to the isolation of 31 sesquiterpenes, featuring mainly the nardosinane and neolemnane carbon skeletons, among which three (13, 14 and 28)...
Source: Chemistry and Biodiversity - February 27, 2024 Category: Biochemistry Authors: Efstathia Ioannou Aikaterini Koutsaviti Marie Kvasnicov á Gabriel Gonzalez Tereza Štenclová Susana Agusti Carlos M Duarte Lucie Rarov á Miroslav Strnad Vassilios Roussis Source Type: research
