In vitro immunotoxicity effects of carbendazim were inhibited by n-acetylcysteine in microglial BV-2 cells
Toxicol In Vitro. 2024 Mar 24;97:105812. doi: 10.1016/j.tiv.2024.105812. Online ahead of print.ABSTRACTCarbendazim (CBZ) is a benzimidazole fungicide widely used worldwide in industrial, agricultural, and veterinary practices. Although, CBZ was found in all brain tissues causing serious neurotoxicity, its impact on brain immune cells remain scarcely understood. Our study investigated the in vitro effects of CBZ on activated microglial BV-2 cells. Lipopolysaccharide (LPS)-stimulated BV-2 cells were exposed to increasing concentrations of CBZ and cytokine release was measured by ELISA, and Cytometric Bead Array (CBA) assays....
Source: Toxicology in Vitro - March 24, 2024 Category: Toxicology Authors: Narjesse E L Mabrouk Maha Mastouri G érard Lizard Mahjoub Aouni Hedi Harizi Source Type: research
In vitro immunotoxicity effects of carbendazim were inhibited by n-acetylcysteine in microglial BV-2 cells
Toxicol In Vitro. 2024 Mar 24;97:105812. doi: 10.1016/j.tiv.2024.105812. Online ahead of print.ABSTRACTCarbendazim (CBZ) is a benzimidazole fungicide widely used worldwide in industrial, agricultural, and veterinary practices. Although, CBZ was found in all brain tissues causing serious neurotoxicity, its impact on brain immune cells remain scarcely understood. Our study investigated the in vitro effects of CBZ on activated microglial BV-2 cells. Lipopolysaccharide (LPS)-stimulated BV-2 cells were exposed to increasing concentrations of CBZ and cytokine release was measured by ELISA, and Cytometric Bead Array (CBA) assays....
Source: Toxicology in Vitro - March 24, 2024 Category: Toxicology Authors: Narjesse E L Mabrouk Maha Mastouri G érard Lizard Mahjoub Aouni Hedi Harizi Source Type: research
In vitro immunotoxicity effects of carbendazim were inhibited by n-acetylcysteine in microglial BV-2 cells
Toxicol In Vitro. 2024 Mar 24;97:105812. doi: 10.1016/j.tiv.2024.105812. Online ahead of print.ABSTRACTCarbendazim (CBZ) is a benzimidazole fungicide widely used worldwide in industrial, agricultural, and veterinary practices. Although, CBZ was found in all brain tissues causing serious neurotoxicity, its impact on brain immune cells remain scarcely understood. Our study investigated the in vitro effects of CBZ on activated microglial BV-2 cells. Lipopolysaccharide (LPS)-stimulated BV-2 cells were exposed to increasing concentrations of CBZ and cytokine release was measured by ELISA, and Cytometric Bead Array (CBA) assays....
Source: Toxicology in Vitro - March 24, 2024 Category: Toxicology Authors: Narjesse E L Mabrouk Maha Mastouri G érard Lizard Mahjoub Aouni Hedi Harizi Source Type: research
In vitro immunotoxicity effects of carbendazim were inhibited by n-acetylcysteine in microglial BV-2 cells
Toxicol In Vitro. 2024 Mar 24;97:105812. doi: 10.1016/j.tiv.2024.105812. Online ahead of print.ABSTRACTCarbendazim (CBZ) is a benzimidazole fungicide widely used worldwide in industrial, agricultural, and veterinary practices. Although, CBZ was found in all brain tissues causing serious neurotoxicity, its impact on brain immune cells remain scarcely understood. Our study investigated the in vitro effects of CBZ on activated microglial BV-2 cells. Lipopolysaccharide (LPS)-stimulated BV-2 cells were exposed to increasing concentrations of CBZ and cytokine release was measured by ELISA, and Cytometric Bead Array (CBA) assays....
Source: Toxicology in Vitro - March 24, 2024 Category: Toxicology Authors: Narjesse E L Mabrouk Maha Mastouri G érard Lizard Mahjoub Aouni Hedi Harizi Source Type: research
In vitro immunotoxicity effects of carbendazim were inhibited by n-acetylcysteine in microglial BV-2 cells
Toxicol In Vitro. 2024 Mar 24;97:105812. doi: 10.1016/j.tiv.2024.105812. Online ahead of print.ABSTRACTCarbendazim (CBZ) is a benzimidazole fungicide widely used worldwide in industrial, agricultural, and veterinary practices. Although, CBZ was found in all brain tissues causing serious neurotoxicity, its impact on brain immune cells remain scarcely understood. Our study investigated the in vitro effects of CBZ on activated microglial BV-2 cells. Lipopolysaccharide (LPS)-stimulated BV-2 cells were exposed to increasing concentrations of CBZ and cytokine release was measured by ELISA, and Cytometric Bead Array (CBA) assays....
Source: Toxicology in Vitro - March 24, 2024 Category: Toxicology Authors: Narjesse E L Mabrouk Maha Mastouri G érard Lizard Mahjoub Aouni Hedi Harizi Source Type: research
In vitro immunotoxicity effects of carbendazim were inhibited by n-acetylcysteine in microglial BV-2 cells
Toxicol In Vitro. 2024 Mar 24;97:105812. doi: 10.1016/j.tiv.2024.105812. Online ahead of print.ABSTRACTCarbendazim (CBZ) is a benzimidazole fungicide widely used worldwide in industrial, agricultural, and veterinary practices. Although, CBZ was found in all brain tissues causing serious neurotoxicity, its impact on brain immune cells remain scarcely understood. Our study investigated the in vitro effects of CBZ on activated microglial BV-2 cells. Lipopolysaccharide (LPS)-stimulated BV-2 cells were exposed to increasing concentrations of CBZ and cytokine release was measured by ELISA, and Cytometric Bead Array (CBA) assays....
Source: Toxicology in Vitro - March 24, 2024 Category: Toxicology Authors: Narjesse E L Mabrouk Maha Mastouri G érard Lizard Mahjoub Aouni Hedi Harizi Source Type: research
N-acetylcysteine regulates oxalate induced injury of renal tubular epithelial cells through CDKN2B/TGF- β/SMAD axis
In conclusion, we found that NAC could play an anti-calculus role by mediating CDKN2B/TGF-β/SMAD axis. (Source: Urolithiasis)
Source: Urolithiasis - March 23, 2024 Category: Urology & Nephrology Source Type: research
Protein phosphatase 4 mediates palmitic acid-induced endothelial dysfunction by decreasing eNOS phosphorylation at serine 633 in HUVECs
This study demonstrated that palmitic acid (PA) induced ED by activating the NADPH oxidase (NOX)/ROS signaling pathway to activate protein phosphatase 4 (PP4) and protein phosphatase 2A (PP2A), thereby reducing endothelial nitric oxide synthase (eNOS) phosphorylation at Ser633 and Ser1177, respectively. Okadaic acid (OA) and fostriecin (FST), which are inhibitors of PP2A, inhibited the PA-induced decreases in eNOS phosphorylation at Ser633 and Ser1177. The antioxidants N-acetylcysteine (NAC) and apocynin (APO) or knockdown of gp91phox or p67phox (NOX subunits) restored PA-mediated downregulation of PP4R2 protein expression...
Source: Atherosclerosis - March 21, 2024 Category: Cardiology Authors: Zhengwei Liang Gang Sun Junshi Zhang Qian Zhang Xiaoyu Li Si Qin Sha Lv Jing Ding Qifang Zhang Yong Xia Deqin Lu Source Type: research
Role of macrophage bioenergetics in N-acetylcysteine-mediated mitigation of lung injury and oxidative stress induced by nitrogen mustard
Toxicol Appl Pharmacol. 2024 Mar 19:116908. doi: 10.1016/j.taap.2024.116908. Online ahead of print.ABSTRACTNitrogen mustard (NM) is a toxic vesicant that causes acute injury to the respiratory tract. This is accompanied by an accumulation of activated macrophages in the lung and oxidative stress which have been implicated in tissue injury. In these studies, we analyzed the effects of N-acetylcysteine (NAC), an inhibitor of oxidative stress and inflammation on NM-induced lung injury, macrophage activation and bioenergetics. Treatment of rats with NAC (150 mg/kg, i.p., daily) beginning 30 min after administration of NM (0.12...
Source: Toxicology and Applied Pharmacology - March 21, 2024 Category: Toxicology Authors: Rama Malaviya Jaclynn Meshanni Vasanthi R Sunil Alessandro Venosa Changjiang Guo Elena V Abramova Kinal N Vayas Chenghui Jiang Jessica A Cervelli Andrew J Gow Jeffrey D Laskin Debra L Laskin Source Type: research
Protein phosphatase 4 mediates palmitic acid-induced endothelial dysfunction by decreasing eNOS phosphorylation at serine 633 in HUVECs
This study demonstrated that palmitic acid (PA) induced ED by activating the NADPH oxidase (NOX)/ROS signaling pathway to activate protein phosphatase 4 (PP4) and protein phosphatase 2A (PP2A), thereby reducing endothelial nitric oxide synthase (eNOS) phosphorylation at Ser633 and Ser1177, respectively. Okadaic acid (OA) and fostriecin (FST), which are inhibitors of PP2A, inhibited the PA-induced decreases in eNOS phosphorylation at Ser633 and Ser1177. The antioxidants N-acetylcysteine (NAC) and apocynin (APO) or knockdown of gp91phox or p67phox (NOX subunits) restored PA-mediated downregulation of PP4R2 protein expression...
Source: Cell Research - March 21, 2024 Category: Cytology Authors: Zhengwei Liang Gang Sun Junshi Zhang Qian Zhang Xiaoyu Li Si Qin Sha Lv Jing Ding Qifang Zhang Yong Xia Deqin Lu Source Type: research
Protein phosphatase 4 mediates palmitic acid-induced endothelial dysfunction by decreasing eNOS phosphorylation at serine 633 in HUVECs
This study demonstrated that palmitic acid (PA) induced ED by activating the NADPH oxidase (NOX)/ROS signaling pathway to activate protein phosphatase 4 (PP4) and protein phosphatase 2A (PP2A), thereby reducing endothelial nitric oxide synthase (eNOS) phosphorylation at Ser633 and Ser1177, respectively. Okadaic acid (OA) and fostriecin (FST), which are inhibitors of PP2A, inhibited the PA-induced decreases in eNOS phosphorylation at Ser633 and Ser1177. The antioxidants N-acetylcysteine (NAC) and apocynin (APO) or knockdown of gp91phox or p67phox (NOX subunits) restored PA-mediated downregulation of PP4R2 protein expression...
Source: Experimental Cell Research - March 21, 2024 Category: Cytology Authors: Zhengwei Liang Gang Sun Junshi Zhang Qian Zhang Xiaoyu Li Si Qin Sha Lv Jing Ding Qifang Zhang Yong Xia Deqin Lu Source Type: research
Protein phosphatase 4 mediates palmitic acid-induced endothelial dysfunction by decreasing eNOS phosphorylation at serine 633 in HUVECs
This study demonstrated that palmitic acid (PA) induced ED by activating the NADPH oxidase (NOX)/ROS signaling pathway to activate protein phosphatase 4 (PP4) and protein phosphatase 2A (PP2A), thereby reducing endothelial nitric oxide synthase (eNOS) phosphorylation at Ser633 and Ser1177, respectively. Okadaic acid (OA) and fostriecin (FST), which are inhibitors of PP2A, inhibited the PA-induced decreases in eNOS phosphorylation at Ser633 and Ser1177. The antioxidants N-acetylcysteine (NAC) and apocynin (APO) or knockdown of gp91phox or p67phox (NOX subunits) restored PA-mediated downregulation of PP4R2 protein expression...
Source: Atherosclerosis - March 21, 2024 Category: Cardiology Authors: Zhengwei Liang Gang Sun Junshi Zhang Qian Zhang Xiaoyu Li Si Qin Sha Lv Jing Ding Qifang Zhang Yong Xia Deqin Lu Source Type: research
Role of macrophage bioenergetics in N-acetylcysteine-mediated mitigation of lung injury and oxidative stress induced by nitrogen mustard
Toxicol Appl Pharmacol. 2024 Mar 19:116908. doi: 10.1016/j.taap.2024.116908. Online ahead of print.ABSTRACTNitrogen mustard (NM) is a toxic vesicant that causes acute injury to the respiratory tract. This is accompanied by an accumulation of activated macrophages in the lung and oxidative stress which have been implicated in tissue injury. In these studies, we analyzed the effects of N-acetylcysteine (NAC), an inhibitor of oxidative stress and inflammation on NM-induced lung injury, macrophage activation and bioenergetics. Treatment of rats with NAC (150 mg/kg, i.p., daily) beginning 30 min after administration of NM (0.12...
Source: Toxicology and Applied Pharmacology - March 21, 2024 Category: Toxicology Authors: Rama Malaviya Jaclynn Meshanni Vasanthi R Sunil Alessandro Venosa Changjiang Guo Elena V Abramova Kinal N Vayas Chenghui Jiang Jessica A Cervelli Andrew J Gow Jeffrey D Laskin Debra L Laskin Source Type: research
Protein phosphatase 4 mediates palmitic acid-induced endothelial dysfunction by decreasing eNOS phosphorylation at serine 633 in HUVECs
This study demonstrated that palmitic acid (PA) induced ED by activating the NADPH oxidase (NOX)/ROS signaling pathway to activate protein phosphatase 4 (PP4) and protein phosphatase 2A (PP2A), thereby reducing endothelial nitric oxide synthase (eNOS) phosphorylation at Ser633 and Ser1177, respectively. Okadaic acid (OA) and fostriecin (FST), which are inhibitors of PP2A, inhibited the PA-induced decreases in eNOS phosphorylation at Ser633 and Ser1177. The antioxidants N-acetylcysteine (NAC) and apocynin (APO) or knockdown of gp91phox or p67phox (NOX subunits) restored PA-mediated downregulation of PP4R2 protein expression...
Source: Experimental Cell Research - March 21, 2024 Category: Cytology Authors: Zhengwei Liang Gang Sun Junshi Zhang Qian Zhang Xiaoyu Li Si Qin Sha Lv Jing Ding Qifang Zhang Yong Xia Deqin Lu Source Type: research
