Epigallocatechin-3-gallate Synergistically Enhanced Arecoline-Induced Cytotoxicity by Redirecting Cycle Arrest to Apoptosis
This study investigated whether epigallocatechin-3-gallate (EGCG), an antioxidant from green tea, could resolve this paradox. Prostate cancer cells (PC-3 cell line) were cultured and treated with arecoline combined with NAC (N-acetylcysteine) or EGCG; the combined effects on intracellular ROS levels and cell viability were examined using the MTT and DCFDA assays, respectively. In addition, apoptosis, cell cycle, and protein expression were investigated using flow cytometry and western blot analysis. Our results showed that EGCG, similar to NAC (N-acetylcysteine), reduced the intracellular ROS levels, which were elevated by...
Source: Current Issues in Molecular Biology - February 23, 2024 Category: Molecular Biology Authors: Li-Jane Shih Po-Chi Hsu Chih-Pin Chuu Hao-Ai Shui Chien-Chih Yeh Yueh-Chung Chen Yung-Hsi Kao Source Type: research
Epigallocatechin-3-gallate Synergistically Enhanced Arecoline-Induced Cytotoxicity by Redirecting Cycle Arrest to Apoptosis
This study investigated whether epigallocatechin-3-gallate (EGCG), an antioxidant from green tea, could resolve this paradox. Prostate cancer cells (PC-3 cell line) were cultured and treated with arecoline combined with NAC (N-acetylcysteine) or EGCG; the combined effects on intracellular ROS levels and cell viability were examined using the MTT and DCFDA assays, respectively. In addition, apoptosis, cell cycle, and protein expression were investigated using flow cytometry and western blot analysis. Our results showed that EGCG, similar to NAC (N-acetylcysteine), reduced the intracellular ROS levels, which were elevated by...
Source: Current Issues in Molecular Biology - February 23, 2024 Category: Molecular Biology Authors: Li-Jane Shih Po-Chi Hsu Chih-Pin Chuu Hao-Ai Shui Chien-Chih Yeh Yueh-Chung Chen Yung-Hsi Kao Source Type: research
Epigallocatechin-3-gallate Synergistically Enhanced Arecoline-Induced Cytotoxicity by Redirecting Cycle Arrest to Apoptosis
This study investigated whether epigallocatechin-3-gallate (EGCG), an antioxidant from green tea, could resolve this paradox. Prostate cancer cells (PC-3 cell line) were cultured and treated with arecoline combined with NAC (N-acetylcysteine) or EGCG; the combined effects on intracellular ROS levels and cell viability were examined using the MTT and DCFDA assays, respectively. In addition, apoptosis, cell cycle, and protein expression were investigated using flow cytometry and western blot analysis. Our results showed that EGCG, similar to NAC (N-acetylcysteine), reduced the intracellular ROS levels, which were elevated by...
Source: Current Issues in Molecular Biology - February 23, 2024 Category: Molecular Biology Authors: Li-Jane Shih Po-Chi Hsu Chih-Pin Chuu Hao-Ai Shui Chien-Chih Yeh Yueh-Chung Chen Yung-Hsi Kao Source Type: research
Epigallocatechin-3-gallate Synergistically Enhanced Arecoline-Induced Cytotoxicity by Redirecting Cycle Arrest to Apoptosis
This study investigated whether epigallocatechin-3-gallate (EGCG), an antioxidant from green tea, could resolve this paradox. Prostate cancer cells (PC-3 cell line) were cultured and treated with arecoline combined with NAC (N-acetylcysteine) or EGCG; the combined effects on intracellular ROS levels and cell viability were examined using the MTT and DCFDA assays, respectively. In addition, apoptosis, cell cycle, and protein expression were investigated using flow cytometry and western blot analysis. Our results showed that EGCG, similar to NAC (N-acetylcysteine), reduced the intracellular ROS levels, which were elevated by...
Source: Current Issues in Molecular Biology - February 23, 2024 Category: Molecular Biology Authors: Li-Jane Shih Po-Chi Hsu Chih-Pin Chuu Hao-Ai Shui Chien-Chih Yeh Yueh-Chung Chen Yung-Hsi Kao Source Type: research
Ascorbic acid induces ferroptosis via STAT3/GPX4 signaling in oropharyngeal cancer
In this study, we demonstrated that AA decreased cell viability and Ki67 expression, along with its accumulation in the G0/G1 phase in FaDu and SCC-154 cell lines. Furthermore, AA exposure induced morphological changes in mitochondria associated with ferroptosis. AA-induced ferroptosis is accompanied by depletion of glutathione (GSH) and increased levels of ferrous ions (Fe2+), reactive oxygen species (ROS), and malondialdehyde (MDA). However, these ferroptotic effects were ameliorated by deferoxamine and N-acetylcysteine. Network pharmacology results showed that signal transducer and activator of transcription 3 (STAT3) i...
Source: Cell Research - February 22, 2024 Category: Cytology Authors: Kaiyuan Wu Le Liu Zhenhua Wu Qi Huang Lei Zhou Rujiao Xie Miao Wang Source Type: research
Uptake of ox-LDL by binding to LRP6 mediates oxidative stress-induced BMSCs senescence promoting obesity-related bone loss
Cell Signal. 2024 Feb 20:111114. doi: 10.1016/j.cellsig.2024.111114. Online ahead of print.ABSTRACTObesity has long been thought to a main cause of hyperlipidemia. As a systemic disease, the impact of obesity on organs, tissues and cells are almost all negative. However, the relationship between obesity and bone loss is most controversial. On the one hand, obesity has long been thought to a positive effect on bone due to increased mechanical loading on the skeleton, conducive to increase bone mass to accommodate the extra weight. On the other hand, obesity-related metabolic oxidative modification of low-density lipoprotein...
Source: Cellular Signalling - February 22, 2024 Category: Cytology Authors: Senxiong Meng Zhuan Wang Xiaonan Liu Ke Shen Yuan Gu Bin Yu Lei Wang Source Type: research
The promise of N-acetylcysteine in the treatment of obsessive-compulsive disorder
CNS Neurosci Ther. 2024 Feb;30(2):e14653. doi: 10.1111/cns.14653.NO ABSTRACTPMID:38385640 | PMC:PMC10883097 | DOI:10.1111/cns.14653 (Source: CNS Neuroscience and Therapeutics)
Source: CNS Neuroscience and Therapeutics - February 22, 2024 Category: Neuroscience Authors: Massimo Carollo Nicola Carollo Giulia Montan Source Type: research
UBE2L3 Promotes Oxidative Stress-regulated Necroptosis to Accelerate Osteosarcoma Progression
CONCLUSION: UBE2L3 is upregulated in osteosarcoma cells, and silencing of UBE2L3 promotes oxidative stress in these cells, leading to enhanced necroptosis and delayed progression of osteosarcoma.PMID:38385491 | DOI:10.2174/0115748928297557240212112531 (Source: Recent Patents on Anti-Cancer Drug Discovery)
Source: Recent Patents on Anti-Cancer Drug Discovery - February 22, 2024 Category: Cancer & Oncology Authors: Xiwu Zhao Guoqiang Shan Deguo Xing Hongwei Gao Zhenggang Xiong Wenpeng Hui Mingzhi Gong Source Type: research
Ascorbic acid induces ferroptosis via STAT3/GPX4 signaling in oropharyngeal cancer
In this study, we demonstrated that AA decreased cell viability and Ki67 expression, along with its accumulation in the G0/G1 phase in FaDu and SCC-154 cell lines. Furthermore, AA exposure induced morphological changes in mitochondria associated with ferroptosis. AA-induced ferroptosis is accompanied by depletion of glutathione (GSH) and increased levels of ferrous ions (Fe2+), reactive oxygen species (ROS), and malondialdehyde (MDA). However, these ferroptotic effects were ameliorated by deferoxamine and N-acetylcysteine. Network pharmacology results showed that signal transducer and activator of transcription 3 (STAT3) i...
Source: Free Radical Research - February 22, 2024 Category: Research Authors: Kaiyuan Wu Le Liu Zhenhua Wu Qi Huang Lei Zhou Rujiao Xie Miao Wang Source Type: research
The promise of N-acetylcysteine in the treatment of obsessive-compulsive disorder
CNS Neurosci Ther. 2024 Feb;30(2):e14653. doi: 10.1111/cns.14653.NO ABSTRACTPMID:38385640 | PMC:PMC10883097 | DOI:10.1111/cns.14653 (Source: CNS Neuroscience and Therapeutics)
Source: CNS Neuroscience and Therapeutics - February 22, 2024 Category: Neuroscience Authors: Massimo Carollo Nicola Carollo Giulia Montan Source Type: research
Uptake of ox-LDL by binding to LRP6 mediates oxidative stress-induced BMSCs senescence promoting obesity-related bone loss
Cell Signal. 2024 Feb 20:111114. doi: 10.1016/j.cellsig.2024.111114. Online ahead of print.ABSTRACTObesity has long been thought to a main cause of hyperlipidemia. As a systemic disease, the impact of obesity on organs, tissues and cells are almost all negative. However, the relationship between obesity and bone loss is most controversial. On the one hand, obesity has long been thought to a positive effect on bone due to increased mechanical loading on the skeleton, conducive to increase bone mass to accommodate the extra weight. On the other hand, obesity-related metabolic oxidative modification of low-density lipoprotein...
Source: Cellular Signalling - February 22, 2024 Category: Cytology Authors: Senxiong Meng Zhuan Wang Xiaonan Liu Ke Shen Yuan Gu Bin Yu Lei Wang Source Type: research
UBE2L3 Promotes Oxidative Stress-regulated Necroptosis to Accelerate Osteosarcoma Progression
CONCLUSION: UBE2L3 is upregulated in osteosarcoma cells, and silencing of UBE2L3 promotes oxidative stress in these cells, leading to enhanced necroptosis and delayed progression of osteosarcoma.PMID:38385491 | DOI:10.2174/0115748928297557240212112531 (Source: Recent Patents on Anti-Cancer Drug Discovery)
Source: Recent Patents on Anti-Cancer Drug Discovery - February 22, 2024 Category: Cancer & Oncology Authors: Xiwu Zhao Guoqiang Shan Deguo Xing Hongwei Gao Zhenggang Xiong Wenpeng Hui Mingzhi Gong Source Type: research
The promise of N-acetylcysteine in the treatment of obsessive-compulsive disorder
CNS Neurosci Ther. 2024 Feb;30(2):e14653. doi: 10.1111/cns.14653.NO ABSTRACTPMID:38385640 | PMC:PMC10883097 | DOI:10.1111/cns.14653 (Source: CNS Neuroscience and Therapeutics)
Source: CNS Neuroscience and Therapeutics - February 22, 2024 Category: Neuroscience Authors: Massimo Carollo Nicola Carollo Giulia Montan Source Type: research
UBE2L3 Promotes Oxidative Stress-regulated Necroptosis to Accelerate Osteosarcoma Progression
CONCLUSION: UBE2L3 is upregulated in osteosarcoma cells, and silencing of UBE2L3 promotes oxidative stress in these cells, leading to enhanced necroptosis and delayed progression of osteosarcoma.PMID:38385491 | DOI:10.2174/0115748928297557240212112531 (Source: Recent Patents on Anti-Cancer Drug Discovery)
Source: Recent Patents on Anti-Cancer Drug Discovery - February 22, 2024 Category: Cancer & Oncology Authors: Xiwu Zhao Guoqiang Shan Deguo Xing Hongwei Gao Zhenggang Xiong Wenpeng Hui Mingzhi Gong Source Type: research
