Protective Effect of < em > N < /em > -Acetylcysteine on Rooster Semen Cryopreservation
Biopreserv Biobank. 2024 Apr 18. doi: 10.1089/bio.2023.0103. Online ahead of print.ABSTRACTCryopreservation of avian semen is a useful reproductive technique in the poultry industry. However, during cooling, elevated reactive oxygen species (ROS) levels have destructive effects on both quality and function of thawed sperm. The aim of the current study is to investigate the antioxidant effects of N-acetylcysteine (NAC) during rooster semen cryopreservation. Semen samples were collected from ten Ross 308 broiler breeder roosters (32 weeks) and mixed. The mixed samples were divided into five equal parts and cryopreserved in L...
Source: Biopreservation and Biobanking - April 18, 2024 Category: Biomedical Science Authors: Farhad Hakimi Mohamad Amir Karimi Torshizi Maryam Hezavehei Mohsen Sharafi Source Type: research
The use of amino acids and their derivates to mitigate against pesticide-induced toxicity
Ecotoxicol Environ Saf. 2024 Apr 17;276:116340. doi: 10.1016/j.ecoenv.2024.116340. Online ahead of print.ABSTRACTExposure to pesticides induces oxidative stress and deleterious effects on various tissues in non-target organisms. Numerous models investigating pesticide exposure have demonstrated metabolic disturbances such as imbalances in amino acid levels within the organism. One potentially effective strategy to mitigate pesticide toxicity involves dietary intervention by supplementing exogenous amino acids and their derivates to augment the body's antioxidant capacity and mitigate pesticide-induced oxidative harm, whose...
Source: Ecotoxicology and Environmental Safety - April 18, 2024 Category: Environmental Health Authors: Guo-Ping Zhao Wei-Long Cheng Zhi-Hui Zhang Yi-Xuan Li Ying-Qiu Li Fang-Wei Yang Yan-Bo Wang Source Type: research
Antioxidant supplementation may effect DNA methylation patterns, apoptosis, and ROS levels in developing mouse embryos
This study was designed to address the question: does antioxidant-containing embryo culture media affect DNA methyltransferases, global DNA methylation, inner cell mass/trophoblast differentiation, intracellular reactive oxygen species (ROS) levels, and apoptosis? Mouse zygotes were cultured in embryo culture media containing MitoQ,N-acetyl-l-cysteine (NAC), acetyl-l-carnitine (ALC), α-lipoic acid (ALA), or the mixture of NAC + ALC + ALA (AO) until the blastocyst stage, whereas in vivo-developed blastocysts were used as control. Protein expression levels of Dnmt1, 3a, 3b, and 3l enzymes were analyzed by immunofluoresc...
Source: Histochemistry and Cell Biology - April 17, 2024 Category: Biomedical Science Source Type: research
Metabolic activation and cytochrome P450 inhibition of piperlonguminine mediated by CYP3A4
This study characterized the O-dealkylated metabolite (M1) through chemical synthesis. The IC50 shift assay showed time-dependent inhibition of CYP3A4, 2C9, 2E1, 2C8 and 2D6 by PLG. This research contributes to the understanding of PLG-induced enzyme inhibition and bioactivation.PMID:38626834 | DOI:10.1016/j.ijbiomac.2024.131502 (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - April 16, 2024 Category: Biochemistry Authors: Yuqian Chi Xiaoliang Zhu Yaxuan Chen Xin Li Ziyi Jiang Xiaoyang Jian Mengyuan Lian Xiaodi Wu Lei Wang Mengmeng Sun Xiaowei Shi Source Type: research
Analysis of Gizzerosine in Foodstuffs by HPLC Involving Pre-column Derivatization with o-Phthaldialdehyde
Adv Exp Med Biol. 2024;1446:237-250. doi: 10.1007/978-3-031-54192-6_11.ABSTRACTGizzerosine [2-amino-9-(4-imidazolyl)-7-azanonanoic acid] is a toxic amino acid formed from histamine and lysine at high temperatures, and may be present in foodstuffs (e.g., fishmeal and meat-bone meal) for animals including cats and dogs. Here we developed a simple, rapid, sensitive, specific, and automated method for the analysis of gizzerosine in foodstuffs by high-performance liquid chromatography (HPLC) involving pre-column derivatization with o-phthaldialdehyde (OPA) in the presence of N-acetylcysteine (instead of the usual 2-mercaptoetha...
Source: Advances in Experimental Medicine and Biology - April 16, 2024 Category: Research Authors: Peng Li Guoyao Wu Source Type: research
Metabolic activation and cytochrome P450 inhibition of piperlonguminine mediated by CYP3A4
This study characterized the O-dealkylated metabolite (M1) through chemical synthesis. The IC50 shift assay showed time-dependent inhibition of CYP3A4, 2C9, 2E1, 2C8 and 2D6 by PLG. This research contributes to the understanding of PLG-induced enzyme inhibition and bioactivation.PMID:38626834 | DOI:10.1016/j.ijbiomac.2024.131502 (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - April 16, 2024 Category: Biochemistry Authors: Yuqian Chi Xiaoliang Zhu Yaxuan Chen Xin Li Ziyi Jiang Xiaoyang Jian Mengyuan Lian Xiaodi Wu Lei Wang Mengmeng Sun Xiaowei Shi Source Type: research
Analysis of Gizzerosine in Foodstuffs by HPLC Involving Pre-column Derivatization with o-Phthaldialdehyde
Adv Exp Med Biol. 2024;1446:237-250. doi: 10.1007/978-3-031-54192-6_11.ABSTRACTGizzerosine [2-amino-9-(4-imidazolyl)-7-azanonanoic acid] is a toxic amino acid formed from histamine and lysine at high temperatures, and may be present in foodstuffs (e.g., fishmeal and meat-bone meal) for animals including cats and dogs. Here we developed a simple, rapid, sensitive, specific, and automated method for the analysis of gizzerosine in foodstuffs by high-performance liquid chromatography (HPLC) involving pre-column derivatization with o-phthaldialdehyde (OPA) in the presence of N-acetylcysteine (instead of the usual 2-mercaptoetha...
Source: Advances in Experimental Medicine and Biology - April 16, 2024 Category: Research Authors: Peng Li Guoyao Wu Source Type: research
