Pediatric Biobanking: Kids Are Not Just Little Adults.
Authors: Catchpoole DR, Carpentieri D, Vercauteren S, Wadhwa L, Schleif W, Zhou L, Zhou J, Labib RM, Smits E, Conradie EH PMID: 32706974 [PubMed - as supplied by publisher] (Source: Biopreservation and Biobanking)
Source: Biopreservation and Biobanking - July 28, 2020 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Returning Results of Stored Biological Samples and Biobanks: Perspectives of Saudi Arabian Biomedical Researchers.
This study aimed to explore the perspectives of health care professionals in their use of stored biological samples for biomedical research regarding the concept of the research results and the challenges of informing donors regarding the results. This qualitative study involved 19 medical researchers doing research with stored biological samples and biobanks. The data were gathered during face-to-face interviews in English using a semistructured interview technique. The participants provided rich and illuminating experiences, framed in the following themes: the professional duty of researchers to return the research resul...
Source: Biopreservation and Biobanking - July 28, 2020 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Aqueous Origanum Vulgare Extract Improves the Quality of Cryopreserved Human Spermatozoa Through Its Antioxidant Effects.
This study aimed at evaluating the antioxidant effects of oregano extract on cryopreserved human spermatozoa. In the first phase, 13 semen samples with different concentrations of oregano extract (0.0, 50, 100, 150, 300, and 500 μg/mL) were cryopreserved to achieve an optimal dose of oregano extract. Then, motility, viability, and plasma membrane integrity were evaluated. In the second phase, 20 samples were cryopreserved in freezing media supplemented with or without the optimal concentration of oregano (100 μg/mL). After thawing, motility, the levels of ROS, lipid peroxidation, and translocation of ph...
Source: Biopreservation and Biobanking - July 28, 2020 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Biopreservation and Biobanking's Mid-Year Update.
Authors: Vaught J PMID: 32700990 [PubMed - as supplied by publisher] (Source: Biopreservation and Biobanking)
Source: Biopreservation and Biobanking - July 24, 2020 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

ISBER in the Time of COVID.
Authors: Seiler CY, Torres A, Simeon-Dubach D PMID: 32667828 [PubMed - as supplied by publisher] (Source: Biopreservation and Biobanking)
Source: Biopreservation and Biobanking - July 17, 2020 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Biochemically Tracked Variability of Blood Plasma Thawed-State Exposure Times in a Multisite Collection Study.
Authors: Hu Y, Mulot C, Bourreau C, Martin D, Laurent-Puig P, Radoï L, Guénel P, Borges CR Abstract The integrity of blood plasma/serum (P/S) specimens can be impacted by preanalytical handling and storage conditions that result in thawed-state exposures (> -30°C). We recently reported a simple dilute-and-shoot, intact-protein liquid chromatography/mass spectrometry (LC/MS) assay called ΔS-Cys-Albumin that quantifies cumulative exposure of P/S to thawed conditions based on the change in relative abundance of the oxidized (S-cysteinylated) proteoform of albumin (S-Cys-Albumin) in the native ...
Source: Biopreservation and Biobanking - July 3, 2020 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Effect of Synergism Between Carboxylated Poly-l-Lysine and Glycerol on Freezability of Nili-Ravi Buffalo (Bubalus bubalis) Semen.
The objective was to evaluate various combinations of CPLL and glycerol in extenders for any synergism toward cryosurvivability of Nili-Ravi buffalo sperm. Semen collected from four Nili-Ravi buffalo bulls (ejaculates = 40; replicates = 5) was diluted in tris-citric acid based extenders, Control: (0% CPLL +7% Glycerol); E1: (1% CPLL +6% Glycerol); E2: (2% CPLL +5% Glycerol); E3: (3% CPLL +4% Glycerol); E4: (4% CPLL +3% Glycerol); E5: (5% CPLL +2% Glycerol); E6: (6% CPLL +1% Glycerol), and E7: (7% CPLL +0% Glycerol), and cryopreserved using a programmable cell freezer. Percentages of post-thaw sp...
Source: Biopreservation and Biobanking - July 3, 2020 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Comparison Between Three Cryoprotectants in the Freezing of Mazama americana Semen Collected by Artificial Vagina.
Authors: Alvarez MCL, Rola LD, Duarte JMB Abstract Maintaining genetic variability is an important part of the conservation of endangered species, so the construction of germplasm banks is essential. Several species of the genus Mazama endure constant pressure in their natural habitat and are threatened with extinction. The correct manipulation and adequacy of the diluents and cryoprotectants must be studied to be successful in the formation of these banks. The purpose of this study was to evaluate the efficiency of three different cryoprotectants in sperm cryopreservation in the species Mazama americana: 6% glycer...
Source: Biopreservation and Biobanking - July 3, 2020 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Testing the Stability of Plasma Protein and Whole Blood RNA in Archived Blood of Loggerhead Sea Turtles, Caretta caretta.
The objective of this study was to examine the stability of plasma proteins and quality of whole blood RNA from loggerhead sea turtle samples collected as part of an eighteen-year-long curated specimen collection. These biological variables are often used to assess sea turtle health; therefore, it is necessary to maintain the integrity of these components during storage. Protein electrophoresis was conducted on heparinized plasma from individual turtles collected in 2018 (n = 3), 2008 (n = 3), and 2001 (n = 3). Plasma was also pooled from four turtles sampled in 2018 and subjected ...
Source: Biopreservation and Biobanking - June 27, 2020 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

The Barriers and Motivators to Using Human Tissues for Research: The Views of UK-Based Biomedical Researchers.
Discussion: We found that there were significant perceptions of governance barriers to sample access. As a consequence, researchers preferred local and known suppliers because of the perception that these could assist with the governance, would be reliable, and able to provide the additional support they required. Equally, data availability was a major contributor to the selection of a new source of samples. These observations are of significant value to those seeking to improve the access to existing sample resources via online discovery tools. PMID: 32551838 [PubMed - as supplied by publisher] (Source: Biopreservation and Biobanking)
Source: Biopreservation and Biobanking - June 21, 2020 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Nonmalignant Formalin-Fixed Paraffin-Embedded Tissues as a Source to Study Germline Variants and Cancer Predisposition: A Systematic Review.
Conclusion: This review illustrates that DNA from nonmalignant FFPE tissues derived from cancer patients can serve as an alternative and reliable source for assessment of germline DNA for various purposes, including assessment of cancer predisposition. PMID: 32551987 [PubMed - as supplied by publisher] (Source: Biopreservation and Biobanking)
Source: Biopreservation and Biobanking - June 21, 2020 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Sodium Alginate as a Novel Cryoprotective Agent for Cryopreservation of Endothelial Cells in a Closed Polytetrafluoroethylene Loop.
In this study, sodium alginate (SA) was employed as one of the main CPAs in a closed polytetrafluoroethylene (PTFE) loop used for cryopreservation with fast freezing of HUVECs. The ice crystal growth process was observed and the thermal enthalpy changes and osmolality of different solutions were tested. Moreover, the effects on cell viability and recovery were examined. The results showed that the addition of SA delayed the growth of ice crystals and decreased the number of ice crystals. Specifically, when 0.5% (w/v) SA was added to the CPAs, the cell survival increased by 10%. It is proved in this study that SA can be use...
Source: Biopreservation and Biobanking - June 21, 2020 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Effect of Quercetin on Oxidative Stress, Mitochondrial Activity, and Quality of Indian Red Jungle Fowl (Gallus gallus murghi) Sperm.
Authors: Rakha BA, Qurrat-Ul-Ain, Ansari MS, Akhter S, Akhter A, Awan MA, Santiago-Moreno J Abstract Aim: The study was designed to elucidate the effects of quercetin as an extender on oxidative stress, mitochondrial activity and quality of Indian red jungle fowl (Gallus gallus murghi) sperm during cryopreservation. Materials and Methods: Semen was collected from seven adult males through abdominal massage and evaluated for semen volume, concentration, and motility. The qualifying semen ejaculates having>80% motility were diluted in red fowl extenders with 0 (control), 5, 10, 15, and 20 mM quercetin. Dilu...
Source: Biopreservation and Biobanking - June 13, 2020 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Dimethyldimethylhydantoin Hydantoin: An Alternative Fluid for Morphological and Genetic Preservation.
Authors: Bourque DA, Morey KC, Bradley DL, Fost B, Daley JM, Jacobson N, Naaum AM, Hanner RH Abstract Modern taxonomy requires the preservation of biospecimens for both morphological and molecular applications. The utility of a previously identified preservative, dimethyldimethylhydantoin hydantoin (Dekafald®), to retain both physical diagnostic traits and the DNA integrity of biological specimens remains unknown. Using 439 eggs and 414 larvae from two North American fish species, we compared three hydantoin solutions at different concentrations (5%, 10%, and 20%) with gold standard preservatives (10% buffered ...
Source: Biopreservation and Biobanking - June 13, 2020 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Hypothermic Storage of Human Umbilical Vein Endothelial Cells and Their Hydrogel Constructs.
Authors: Zhang X, Cao Y, Zhao G Abstract Human umbilical vein endothelial cells (HUVECs) have great potential in tissue engineering, regenerative medicine, and clinical applications. There is an ever-increasing demand to provide living HUVECs and HUVECs-hydrogel constructs to end users when needed in cell-based therapy and clinical applications. However, current methods to provide living cells and their constructs are mainly continuous culture and cryopreservation, which are high-cost, labor-intensive, time-consuming and inflexible. The research about hypothermic storage of HUVECs and their hydrogel constructs is s...
Source: Biopreservation and Biobanking - June 13, 2020 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Best Practices for Human Biobank Ethics Review in China.
Authors: Liu S, Wang WC, Zhang H, Tong J, Zhang X, Chen P, Hu C Abstract The ethical practices for human biobanks in China are intended to safeguard the interests of all the participants, to standardize the construction, management, and resource sharing of human biobanks, to promote the development of medical research, and to improve public health and well-being. The practices contain several chapters: General Principles; Ethics Review; Informed Consent; Privacy Protection; Benefits of Sharing; and Conflict of Interest. PMID: 32429687 [PubMed - as supplied by publisher] (Source: Biopreservation and Biobanking)
Source: Biopreservation and Biobanking - May 22, 2020 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Effects of Freezing and Thawing Procedures on Selected Clinical Chemistry Parameters in Plasma.
In this study, we aimed at assessing the effects of different freezing and thawing conditions on the reproducibility of medical routine parameters from frozen samples. Materials and Methods: In total, 12 pooled samples were generated from leftover lithium heparinized plasma samples from clinical routine testing. Aliquots of the pools were frozen using three freezing methods (in carton box at -80°C, flash freezing in liquid nitrogen, and controlled-rate freezing [CRF]) and stored at -80°C. After 3 days, samples were thawed using two methods (30 minutes at room temperature or water bath at 25°C for 3 minutes). Te...
Source: Biopreservation and Biobanking - May 22, 2020 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Effects of Recombinant AavLEA1 Protein on Human Umbilical Cord Matrix Mesenchymal Stem Cells Survival During Cryopreservation.
In conclusion, recombinant AavLEA1 protein can improve the efficiency of MSC cryopreservation, increase the hUCM-MSCs viability, and partly replace DMSO during cryopreservation. PMID: 32423228 [PubMed - as supplied by publisher] (Source: Biopreservation and Biobanking)
Source: Biopreservation and Biobanking - May 21, 2020 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Moving ISBER into the Future: Looking Beyond Our Horizons.
Authors: Catchpoole D PMID: 32392423 [PubMed - as supplied by publisher] (Source: Biopreservation and Biobanking)
Source: Biopreservation and Biobanking - May 12, 2020 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Hydrogel Microfiber Encapsulation Enhances Cryopreservation of Human Red Blood Cells with Low Concentrations of Glycerol.
This study validated the feasibility of cryopreservation of RBC-laden hydrogel microfibers. It may provide a new and evolving direction for reducing the concentrations of CPAs used in the preservation of RBCs. PMID: 32315536 [PubMed - as supplied by publisher] (Source: Biopreservation and Biobanking)
Source: Biopreservation and Biobanking - April 22, 2020 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Biobanking During the COVID-19 Pandemic.
Authors: Vaught J PMID: 32297797 [PubMed - as supplied by publisher] (Source: Biopreservation and Biobanking)
Source: Biopreservation and Biobanking - April 18, 2020 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Extending the Minimum Information About BIobank Data Sharing Terminology to Describe Samples, Sample Donors, and Events.
Discussion: The MIABIS sample and donor component extensions and the new generic data model complement the existing MIABIS Core 2.0 components, and substantially increase the potential usability of this terminology for better describing biobank samples and sample donors. They also support the use of individual level data about samples and sample donors to obtain accurate and detailed biobank availability queries. PMID: 32302498 [PubMed - as supplied by publisher] (Source: Biopreservation and Biobanking)
Source: Biopreservation and Biobanking - April 18, 2020 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Biobanking for Genomic and Personalized Health Research: Participant Perceptions and Preferences.
Conclusions: Our results suggest that while there is room for improvement in providing adequate information to enable participants' understanding of the purpose of GAPH studies such as SpecTRA, patients are supportive of GAPH in general. Results also suggest that willingness to participate would likely be impacted by factors such as the study's commercial and national affiliations. This study indicates that further work is required to guide improvements on how the GAPH research community describes studies to potential participants, and to enable participation options that incorporate variable participant preferences. P...
Source: Biopreservation and Biobanking - April 18, 2020 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Cryopreservation of Viable Human Tissues: Renewable Resource for Viable Tissue, Cell Lines, and Organoid Development.
In this study, we validated 2D and 3D tissue cultures as methods to corroborate the feasibility of viable cryopreservation of tumor tissue. This proof-of-principle study, if more widely implemented, should improve accessibility of human viable tumor tissue/cells in a time-independent manner for many basic, preclinical, and translational assays. PMID: 32302515 [PubMed - as supplied by publisher] (Source: Biopreservation and Biobanking)
Source: Biopreservation and Biobanking - April 18, 2020 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Towards Harmonized Biobanking for Biomonitoring: A Comparison of Human Biomonitoring-Related and Clinical Biorepositories.
Authors: Lermen D, Gwinner F, Bartel-Steinbach M, Mueller SC, Habermann JK, Balwir MB, Smits E, Virgolino A, Fiddicke U, Berglund M, Åkesson A, Bergstrom A, Leander K, Horvat M, Snoj Tratnik J, Posada de la Paz M, Castaño Calvo A, Esteban López M, von Briesen H, Zimmermann H, Kolossa-Gehring M Abstract Human biomonitoring (HBM) depends on high-quality human samples to identify status and trends in exposure and ensure comparability of results. In this context, much effort has been put into the development of standardized processes and quality assurance for sampling and chemical analysis, while ef...
Source: Biopreservation and Biobanking - April 15, 2020 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Introduction to New Section: Emerging Markets and Technologies.
Authors: Gupta R, Brooks A PMID: 32281896 [PubMed - in process] (Source: Biopreservation and Biobanking)
Source: Biopreservation and Biobanking - April 15, 2020 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Cryopreservation and Culture of Testicular Tissues: An Essential Tool for Biodiversity Preservation.
The objective of this review was to describe current advances, limitations, and perspectives related to the use of testicular tissue preservation as a strategy for the conservation of male fertility. Testes can be obtained from mature or prepubertal individuals, immediately postmortem or by orchiectomy, but testicular biopsies could also be an alternative to collect samples from living individuals. Testicular fragments can be then cryopreserved by using slow or ultra-rapid freezing, or even vitrification methods. The composition of cryopreservation media can vary according to species-specific characteristics, especially re...
Source: Biopreservation and Biobanking - April 15, 2020 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Genotypic and Phenotypic Stability of Mixed Primary Isolates of Cryptococcus gattii and Cryptococcus neoformans: A Comparative Analysis of Four Preservation Methods.
Authors: Nascimento CRSD, Souto ADSS, Galvão RM, Lazéra MDS, Trilles L Abstract The choice of a suitable preservation method is critical for long-term microorganisms' viability. The strains should be preserved for long periods using reliable and reproducible methods that minimize genotypic and phenotypic variations and viability losses. The methodologies are usually designed for a better performance in isolated microorganisms. However, atypical primary isolates of Cryptococcus neoformans or Cryptococcus gattii, such as mixed species or even different species of a species complex, are a challenge for l...
Source: Biopreservation and Biobanking - March 29, 2020 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Effect of Equilibration Time and Temperature on Murine Spermatogonial Stem Cell Cryopreservation.
This study aimed to establish a standard equilibration protocol to improve the cryopreservation efficiency of murine germ cells enriched for SSCs. After time- and temperature-dependent equilibration, the germ cells were cryopreserved with 10% dimethyl sulfoxide (DMSO) and 200 mM trehalose. To investigate cryopreservation efficiency at different equilibration conditions, the survival and proliferation rates were assessed after thawing, and then, cytotoxicity and intracellular trehalose quantification were analyzed. Protein (PLZF, GFRα1, VASA, and c-Kit) and gene (Bcl6b, Erm, Dazl, and Sycp1) expression was dete...
Source: Biopreservation and Biobanking - March 29, 2020 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Patients' Knowledge and Attitude Toward Biobanks in Eastern Morocco.
Conclusion: Despite the lack of knowledge of biobanks among patients in Eastern Morocco, the majority of them expressed willingness to participate in biobanking through donation of biospecimens. However, active participation depended upon a number of factors, notably, the trust in biomedical research and privacy. Therefore, more efforts are needed to increase awareness and promote wider participation in biobanking. PMID: 32207985 [PubMed - as supplied by publisher] (Source: Biopreservation and Biobanking)
Source: Biopreservation and Biobanking - March 26, 2020 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

A Pilot Cancer-Phenome Biobanking System in a Low-Resource Southeast Asian Setting: The Philippine General Hospital Biobank Experience.
In this study, we present a specific use case of biobanking activity at the PGH Biobank, to build a cohort of biospecimens from Filipino patients with breast, endometrial, and ovarian cancer. This initiative is part of a biomonitoring study (1) to assess environmental exposures and possible risk factors in the Philippine population and (2) to develop a system of culturing human cells from Filipino patients for subsequent in vitro studies. We discuss issues faced and the solutions developed during the implementation of the biobank. Strong research collaboration, a funding source, basic infrastructure, and appropriate techno...
Source: Biopreservation and Biobanking - March 25, 2020 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

ISBER 2020 Awards.
Authors: Lewandowski D PMID: 32196352 [PubMed - as supplied by publisher] (Source: Biopreservation and Biobanking)
Source: Biopreservation and Biobanking - March 22, 2020 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Quality Management Practices of Gene Banks for Livestock: A Global Review.
The objective of this study was to review the quality management procedures of livestock gene banks around the world to identify the general strengths and weaknesses of quality control. A survey was administered by means of an online questionnaire consisting of 54 questions, most of which were yes/no with respect to the presence of a particular aspect of quality management. The survey was distributed through networks of the Food and Agriculture Organization of the United Nations that are associated with AnGR. Ninety responses were received from 62 countries. The gene banks were predominantly public institutions, with the m...
Source: Biopreservation and Biobanking - March 5, 2020 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

The Replacement of Fetal Bovine Serum with Bovine Serum Albumin During Oocyte Maturation and Embryo Culture Does Not Improve Blastocyst Quality After Slow Freezing Cryopreservation.
Authors: Sena-Netto SB, Sprícigo JFW, Leme LO, Guimarães ALS, Caixeta FMC, Dode MAN, Pivato I Abstract In the present study, four experimental groups were used: fresh embryos, cultured during in vitro maturation and in vitro culture in media supplemented with bovine serum albumin (BSA) (fresh BSA) or fetal bovine serum (FBS) (fresh FBS); and two groups of cryopreserved and thawed embryos, produced under the same conditions (frozen BSA and frozen FBS). Experiment 1 evaluated the protein source effect on embryo development and response to cryopreservation. At day 7, half of the expanded blastocysts (Bx)...
Source: Biopreservation and Biobanking - February 28, 2020 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Effects of Vitrification Techniques on the Somatic Tissue Preservation of Agouti (Dasyprocta leporina Linnaeus, 1758).
Authors: Costa CAS, Borges AA, Nascimento MB, Aquino LVC, Silva AR, Oliveira MF, Pereira AF Abstract The cryobanks of agouti somatic tissues represent a promising tool for the conservation of this species and of those that are phylogenetically related and endangered. For these purposes, one strategy to guarantee the quality of samples after warming would be to choose the appropriate tissue vitrification technique. Therefore, we evaluated the effects of two different techniques, direct vitrification in cryovials (DVC) and solid-surface vitrification (SSV), on the preservation of ear somatic tissues derived from agou...
Source: Biopreservation and Biobanking - February 22, 2020 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Feasibility Analysis of Cell-Free DNA Derived from Plasma of Lung Cancer Patients for Next-Generation Sequencing.
Conclusion: Frozen lung cancer plasma samples stored in the biobank for 1-6 years at -80°C under certain conditions still retain a high level of cfDNA, which is suitable for NGS detection. PMID: 32083487 [PubMed - as supplied by publisher] (Source: Biopreservation and Biobanking)
Source: Biopreservation and Biobanking - February 22, 2020 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

The Experts Speak on Biobank Education.
Authors: O'Donoghue S, Byrne JA, Green C, Hill K, Kozlakidis Z, De Wilde A, Mullins P, Wiles KR PMID: 32069096 [PubMed - in process] (Source: Biopreservation and Biobanking)
Source: Biopreservation and Biobanking - February 20, 2020 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

UBCx "Biospecimen Research Methods Course".
UBCx "Biospecimen Research Methods Course". Biopreserv Biobank. 2020 Feb;18(1):4-6 Authors: Matzke L, O'Donoghue S, Watson PH PMID: 32069097 [PubMed - in process] (Source: Biopreservation and Biobanking)
Source: Biopreservation and Biobanking - February 20, 2020 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Integrated BioBank of Luxembourg-University of Luxembourg: University Biobanking Certificate.
Authors: Castellanos-Uribe M, May ST, Betsou F PMID: 32069098 [PubMed - in process] (Source: Biopreservation and Biobanking)
Source: Biopreservation and Biobanking - February 20, 2020 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

The International Teaching and Practice of Cryobiology and Biobankology Course in China.
Authors: Zhou H, Wu X, Zeng L, Yan X, Tian W, Xiong K, Huang J Abstract In the past 10 years, clinical biobanks have experienced increasing expansion in China. Demand for systematically educated biobanking professionals is a priority for Chinese biobanks' agenda. The cryobiology and biobankology course is the first semester-long course in China, designed and developed at Central South University with international cooperation. Leading professors were from China, the United States, United Kingdom, and Canada to teach the latest version of biobanking knowledge and skills around the globe. This course is a comprehensi...
Source: Biopreservation and Biobanking - February 20, 2020 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Enhancing Cooperation Between Academic Biobanks and Biomedical Industry: Better Mutual Understanding and New Collaborative Models Are Needed.
Authors: Simeon-Dubach D, Roehrl MH, Hofman P, Puchois P PMID: 32043910 [PubMed - as supplied by publisher] (Source: Biopreservation and Biobanking)
Source: Biopreservation and Biobanking - February 12, 2020 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Method Validation for Extraction of DNA from Human Stool Samples for Downstream Microbiome Analysis.
Conclusions: We validated a combination of a stool processing method with various collection methods, suitable for downstream microbiome applications. Sample collection, storage conditions and DNA extraction methods can influence the microbiome profile results. Laboratories and biobanks should ensure that these conditions are systematically recorded in the scope of accreditation. PMID: 31999474 [PubMed - as supplied by publisher] (Source: Biopreservation and Biobanking)
Source: Biopreservation and Biobanking - February 1, 2020 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Comparison and Analysis of Two Internationally Recognized Biobanking Standards.
We examined the CTRNet Required Operational Practices (2017) and ISO 20387 (2018), to compare them. Although the organization of each standard is different, both describe a set of discrete requirements (elements or subclauses) that comprise the standards that are contained in sections called chapters (CTRNet) or clauses (ISO). The standards have a similar number of requirements (CTRNet: 362, ISO: 322). To compare these standards, we reclassified the requirements in the ISO standard into 13 categories based on a combination of the chapter headings used in the ISBER and NCI Best Practices that represent important areas of bi...
Source: Biopreservation and Biobanking - January 29, 2020 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

A New Qualification for the New Year: ISBER and American Society of Clinical Pathology Board of Certification Announce New Qualification in Biorepository Science Examination for Biobank Technicians.
Authors: Schacter B, Sieffert N, Hill K, Tanabe P, Simeon-Dubach D PMID: 31990588 [PubMed - as supplied by publisher] (Source: Biopreservation and Biobanking)
Source: Biopreservation and Biobanking - January 29, 2020 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Histological and Biomechanical Characteristics of Human Decellularized Allograft Heart Valves After Eighteen Months of Storage in Saline Solution.
Authors: Wollmann LC, Suss PH, Kraft L, Stadler Ribeiro V, Noronha L, da Costa FDA, Tuon FF Abstract Background: The best storage preservation method for maintaining the quality and safety of human decellularized allograft heart valves is yet to be established. Objective: The aim of the present study was to evaluate the stability in terms of extracellular matrix (ECM) integrity of human heart valve allografts decellularized using sodium dodecyl sulfate-ethylenediaminetetraacetic acid (SDS-EDTA) and stored for 6, 12, and 18 months. Methods: A total of 70 decellularized aortic and pulmonary valves were analyzed acros...
Source: Biopreservation and Biobanking - January 29, 2020 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Brain Organoids: A Promising Living Biobank Resource for Neuroscience Research.
Authors: Li S, Wang M, Zhou J Abstract Biobanking plays an important role between clinical practice and translational research. In addition to the traditional biomolecular-based biobanks, there is a growing interest in establishing living biobanks, including organoid biobanks that can collect and store viable and functional tissues and proliferative cell types for long periods of time. An organoid is a three-dimensional cell complex derived by self-organization of small tissue blocks or stem cells, which can recapitulate the phenotypic and genetic characteristics of targeted human organs. Publications on brain orga...
Source: Biopreservation and Biobanking - January 26, 2020 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Special Section on Biobanking Education.
Authors: Castellanos M, Gormally E, Zhou H, Matzke E, Watson P PMID: 31971815 [PubMed - as supplied by publisher] (Source: Biopreservation and Biobanking)
Source: Biopreservation and Biobanking - January 25, 2020 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Utilization of Mobile Application for Better Implementation of Good Clinical Practice in a Biorepository Sample Collection Process: Functions of PancMoBio in Biobanking.
Authors: Tian L, Zhang K, Wang X, Jiang K, Gao W, Liang N, Wang X, Miao Y, Lu Z Abstract Preanalytical variables of biospecimens play a vital role in biobanking. Currently, there is a lack of a convenient and precise methods to document these variables. Paper documentation and computer-based Lab Information Management System software are the most common solutions, but both have clear disadvantages. An application named PancMoBio was newly developed in our pancreas biobank, with the guidance of good clinical practice principles as well as the incorporation of technical support from professional software companies. W...
Source: Biopreservation and Biobanking - January 9, 2020 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Biosafety Evaluation of Equine Umbilical Cord-Derived Mesenchymal Stromal Cells (UC-MSCs) by Systematic Pathogen Screening in Peripheral Maternal Blood and Paired UC-MSCs.
Conclusions: In-process microbiological controls combined with PMB PCR screening provide a comprehensive assessment of UC-MSC exposure to infectious risk, vertical transmission risk appearing inherently low. PMID: 31904273 [PubMed - as supplied by publisher] (Source: Biopreservation and Biobanking)
Source: Biopreservation and Biobanking - January 8, 2020 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Modeling Clinical Processes to Consent Research Donors of Remnant Biospecimens in an Outpatient Cardiology Clinic.
Conclusions: BURRITO electronically documents informed consent using an EMR functionality and the least amount of clinical and research resources. Our results show promise for developing institutionally adopted processes, which could leverage existing clinical workflows for universal research consenting and scalability. PMID: 31880471 [PubMed - as supplied by publisher] (Source: Biopreservation and Biobanking)
Source: Biopreservation and Biobanking - December 29, 2019 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research