Evaluation of Enrofloxacin for Use in Cryopreservation of Zebu Bull (Bos indicus) Semen.
Authors: Ishaq R, Ansari MS, Rakha BA, Qadeer S, Akhter S Abstract Aim: The present study was designed to evaluate enrofloxacin (EN), a fluoroquinolone broad-spectrum antibacterial drug for use in cryopreservation of Zebu bull semen. Materials and Methods: Semen was collected from three Zebu bulls of the Sahiwal breed (Bos indicus) for 3 weeks (replicates) for each experiment. Experiment I: semen samples were cultured for isolation of bacteria and in vitro sensitivity tests against streptomycin, penicillin, and EN. Proteus spp., Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli were isolated. P. a...
Source: Biopreservation and Biobanking - June 22, 2019 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Great Lakes Biorepository Research Network's Annual Biobanking Symposium: A Focus on Precision Medicine.
Authors: Paulauskis JD, Blanc VM, Carey T, Chesla DW, Frey RC, Geddes T, Keats J, Loup A, Pruetz B, Rohrer DC, Valley DR, Tomlinson T, Akervall J, Wilson GD, Jewell SD PMID: 31211591 [PubMed - as supplied by publisher] (Source: Biopreservation and Biobanking)
Source: Biopreservation and Biobanking - June 19, 2019 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Cryopreservation of a Thermotolerant Lineage of the Coral Reef Dinoflagellate Symbiodinium.
Authors: Lin C, Thongpoo P, Juri C, Wang LH, Meng PJ, Kuo FW, Tsai S Abstract Dinoflagellates of the genus Symbiodinium form symbiotic relationships with corals, other marine invertebrates, and protists; thus, they are considered as important species in coral reef ecosystems. If Symbiodinium could be successfully cryopreserved, the cell bank generated could prove to be a valuable resource for researchers interested in basic biological research of Symbiodinium-invertebrate symbioses. Herein, successful cryopreservation of clade D Symbiodinium was achieved using a two-step freezing protocol. Symbiodinium cells were e...
Source: Biopreservation and Biobanking - June 15, 2019 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

miR-200c-3p Suppresses the Proliferative, Migratory, and Invasive Capacities of Nephroblastoma Cells via Targeting FRS2.
Conclusions: miR-200c-3p suppresses the malignant behaviors of nephroblastoma cells by downregulating the expression of FRS2. PMID: 31194576 [PubMed - as supplied by publisher] (Source: Biopreservation and Biobanking)
Source: Biopreservation and Biobanking - June 15, 2019 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Canadian Tissue Repository Network Biobank Certification Program: Update and Review of the Program from 2011 to 2018.
Authors: Hartman V, Gali B, Dee S, O'Donoghue S, Tarling T, Barnes R, de Ladurantaye M, Mes-Masson AM, Watson PH Abstract The Canadian Tissue Repository Network (CTRNet) Biobank Certification Program was first launched in 2011 to foster translational research through improved access to high quality biospecimens. This was accomplished by creating and providing biobank education and through the establishment and deployment of common standards to harmonize biospecimen quality and approaches to governance. The CTRNet program comprises registration and certification steps as two linked phases. In the two-step registrati...
Source: Biopreservation and Biobanking - June 15, 2019 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Optimization of Tissue Microarrays from Banked Human Formalin-Fixed Paraffin Embedded Tissues in the Cancer Research Setting.
In this study, we report a stepwise optimization of TMA generation parameters, including paraffin wax selection, tempering protocol, and sectioning conditions, to achieve the best ribbon sectioning. PMID: 31194582 [PubMed - as supplied by publisher] (Source: Biopreservation and Biobanking)
Source: Biopreservation and Biobanking - June 15, 2019 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Ensuring Effective Utilization of Biospecimens: Design, Marketing, and Other Important Approaches.
This article discusses the impact of these factors on biospecimen utilization and provides suggestions for how bioresources can optimize biospecimen utilization from their collections. PMID: 31188625 [PubMed - in process] (Source: Biopreservation and Biobanking)
Source: Biopreservation and Biobanking - June 14, 2019 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

The Importance of Human Tissue Bioresources in Advancing Biomedical Research.
Authors: Al Diffalha S, Sexton KC, Watson PH, Grizzle WE Abstract Medical research advances enabling the realization of precision medicine have relied heavily on the biospecimens provided by bioresources to identify the targets and biomarkers that are the focus of the new generation of more effective molecular-based therapies for specific subtypes of diseases. Through the biospecimens they have distributed, bioresources have permitted subtypes of cancers to be identified and molecular features of these subtypes to be effectively targeted. A prototype example is the human epidermal growth factor receptor type 2 (HER...
Source: Biopreservation and Biobanking - June 14, 2019 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

The Utilization of Biospecimens: Impact of the Choice of Biobanking Model.
This article discusses some of the different models that may be used alone or in combination by a bioresource providing biospecimens for research; it describes the factors affecting the choice of the most appropriate model or models, the advantages and disadvantages of the various models, and a discussion of the impact of the choice of the model on biospecimen utilization. Frequently, problems with biospecimen utilization are not caused by any single model, but rather a mismatch between the choice of model and goals of the bioresource, and/or problems with the subsequent design, goals, operations, and management of the bio...
Source: Biopreservation and Biobanking - June 14, 2019 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Biobank: What's in a Name?
Authors: Watson PH, Hewitt RE, Catchpoole DR, Grizzle WE PMID: 31188628 [PubMed - in process] (Source: Biopreservation and Biobanking)
Source: Biopreservation and Biobanking - June 14, 2019 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Biological, Medical, and Other Tissue Variables Affecting Biospecimen Utilization.
This article also describes current challenges related to biospecimen characteristics that may affect biospecimen utilization. These include inadequate awareness of investigators about the availability of biospecimens with specific morphologic and molecular features, donor variability, preanalytical variables, technical problems inherent with an investigator's request for biospecimens, limited tissue availability from a biorepository based on requested sizes and/or numbers of available biospecimens, effects of times of warm and cold ischemia, damage of tissues during surgery, and molecular changes during storage. To ensure...
Source: Biopreservation and Biobanking - June 14, 2019 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Advancing Professionalization of Biobank Business Operations: Performance and Utilization.
Authors: Henderson MK, Goldring K, Simeon-Dubach D Abstract Biobanks are now in the spotlight as key enablers supporting preclinical, clinical, and environmental research. Awareness of their value has increased along with the need for these infrastructures to be sustained through business-focused practices. Following our 2017 pilot survey on biobank business planning, we initiated a more comprehensive 38-question multiple-language worldwide survey on biobank sustainability. Two hundred seventy-six biobanks of various sizes and stages of business planning (in place, in progress or none) responded. About two-thirds w...
Source: Biopreservation and Biobanking - June 14, 2019 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Biospecimen Utilization: A Critical Challenge in Global Bioresource/Biobanking Operations.
Authors: Bledsoe MJ, Grizzle WE PMID: 31188631 [PubMed - in process] (Source: Biopreservation and Biobanking)
Source: Biopreservation and Biobanking - June 14, 2019 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Biospecimen Complexity and the Evolution of Biobanks.
Authors: Hartman V, Matzke L, Watson PH Abstract Biospecimens are critical in driving health research. There is increased demand for scale and quality of biospecimens that in turn drives biobanking operational costs, influences utilization, and threatens the sustainability of individual biobanks. Biospecimen research has begun to inform the details of new biobanking standards and the steps of the biobanking process that are most important to focus on to achieve higher quality. This focus on quality is currently centered mostly on intrinsic features of biospecimens and their annotating data. This review highlights a...
Source: Biopreservation and Biobanking - June 14, 2019 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

The ISBER 2019 Annual Meeting and Exhibits.
Authors: Gan R, Pazahanick A, Kozlakidis Z PMID: 31188633 [PubMed - in process] (Source: Biopreservation and Biobanking)
Source: Biopreservation and Biobanking - June 14, 2019 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Monitoring Dehydration and Clearing in Tissue Processing for High-Quality Clinical Pathology.
In this study, we expand the use of this technology to monitor tissue dehydration and clearing by analyzing TOF signals from 270 different specimens, representing 13 different tissue types obtained through surgical resections. We determined the time constant τ90 for each tissue type for the following tissue processing solvents: 70% ethanol, 90% ethanol, 100% ethanol, and xylene. The TOF signals were correlated with tissue morphology to ensure that high-quality tissue was produced. Tissues can be grouped into those exhibiting fast and slow reagent diffusion. We monitored incomplete dehydration of tissue by skipping a ke...
Source: Biopreservation and Biobanking - May 22, 2019 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Centralization of the IARC Biobank: Combining Multiple Sample Collections into a Common Platform.
Authors: Mendy M, Caboux E, Wild CP, IARC Biobank Steering Committee Members Abstract The International Agency for Research on Cancer (IARC) is the World Health Organization's (WHO) cancer research agency. The agency conducts research on cancer with worldwide collaborations, adopting a multidisciplinary approach of epidemiology and laboratory-based studies on cancer causes, as well as preventive interventions. The IARC Biobank stores multiple collections of samples and conducts preanalytical services for studies conducted worldwide in support of the research activities. Traditionally, the multiple collections from ...
Source: Biopreservation and Biobanking - May 16, 2019 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Biospecimen Data Reporting in the Research Literature.
In this study we evaluated the uptake and reporting of BRISQ criteria in 324 articles across four leading biomedical journals using human biospecimens and published before (161; in 2010) and after (163; in 2014) the delineation of the BRISQ guidelines. We found that even within journals recommending use of BRISQ, manuscript-level uptake. and reporting of the relevant biospecimen information is not widespread or uniform. In the future, an enhanced biospecimen reporting strategy to better serve the needs of researchers, reviewers, and journals may be considered to strengthen research reproducibility for the benefit of the re...
Source: Biopreservation and Biobanking - May 2, 2019 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Parathyroid Tissue Cryopreservation: Does the Storage Time Affect Viability and Functionality?
Authors: Aiti A, Rossi M, Alviano F, Morara B, Burgio L, Cioccoloni E, Cavicchi O, Pasquinelli G, Bonsi L, Buzzi M Abstract Parathyroidectomy is a standard practice to treat recurrent or persistent hyperparathyroidism. However, this can lead to the onset of hypoparathyroidism, treatable with the autotransplantation of parathyroid tissue (PT). Tissue can be transplanted immediately after parathyroidectomy or cryopreserved and transplanted only in case of necessity. Since 2011, the Cord Blood Bank and Cardiovascular Tissue Bank of Emilia-Romagna has been storing PT for potential autologous transplantation. To date, t...
Source: Biopreservation and Biobanking - April 28, 2019 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Temporary Ischemia Time Before Snap Freezing Is Important for Maintaining High-Integrity RNA in Hepatocellular Carcinoma Tissues.
Conclusions: Tissue quality is adversely affected by ischemia time and ischemia temperature. Therefore, temporary ischemia time (≤15 minutes) before snap freezing is key for maintaining high-integrity RNA in HCC tissues. PMID: 31025876 [PubMed - as supplied by publisher] (Source: Biopreservation and Biobanking)
Source: Biopreservation and Biobanking - April 28, 2019 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Biobank Continuity Management: A Survey of Biobank Professionals.
Authors: Church TD, Richmond FJ Abstract Academic biobanks face challenges that call for continuity and disaster planning. However, current regulations do not require such planning, so it is unclear if and how biobanks have prepared themselves to deal with future crises. This exploratory study used mixed methods to understand the state of continuity planning in U.S. biobanks. It first reviewed the current state of regulatory and implementation requirements that drive and challenge continuity planning. A survey instrument was then developed and critiqued by a focus group of experienced practitioners in biobanking. T...
Source: Biopreservation and Biobanking - April 26, 2019 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Quality Management System in the BBMRI.pl Consortium: Status Before the Formation of the Polish Biobanking Network.
Authors: Ferdyn K, Gleńska-Olender J, Witoń M, Zagórska K, Kozera Ł, Chróścicka A, Matera-Witkiewicz A Abstract Many types of biomedical research projects depend on high-quality biological material with a data set attached. The Quality Management System (QMS) is focused on operational standards for all organizational activities to ensure that the described quality of each procedure, product, or service is guaranteed. The implementation of the QMS is necessary for the provision of both high quality and repeatability of processes in research laboratories. The current status of implementation of the ...
Source: Biopreservation and Biobanking - April 23, 2019 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Development of a Biobank from a Legacy Collection in Universitas Gadjah Mada, Indonesia: Proposed Approach for Centralized Biobank Development in Low-Resource Institutions.
Conclusion: This report demonstrated current technical and nontechnical insights to further develop a centralized biobank for health research at an academic institution in Indonesia. PMID: 31009252 [PubMed - as supplied by publisher] (Source: Biopreservation and Biobanking)
Source: Biopreservation and Biobanking - April 23, 2019 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Effect of Knockout Serum Replacement During Postwarming Recovery Culture on the Development and Quality of Vitrified Parthenogenetic Porcine Blastocysts.
In conclusion, our data showed that KSR supplementation during recovery culture can improve the development and quality of vitrified parthenogenetic porcine blastocysts. These findings provide a useful reference that KSR could be used to replace FBS as a defined serum supplement for recovery culture of vitrified blastocysts. PMID: 31009253 [PubMed - as supplied by publisher] (Source: Biopreservation and Biobanking)
Source: Biopreservation and Biobanking - April 23, 2019 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Gene Mutations in Ganoderma lucidum During Long-Term Preservation by Repeated Subculturing.
In this study, to assess and verify the risks of repeated subculturing on the long-term preservation of strains of culture collections, we performed single nucleotide polymorphism (SNP) analysis in genes encoding enzymes of the mevalonate pathway, 1,3-β-glucan synthesis, lignin degradation, and the tricarboxylic acid (TCA) cycle of mycelia before and after preserving for a 4-year period by the subculturing 30 times every 45 days of Ganoderma lucidum NBRC 8346. As a result of analyzing 60 genes of the strain, SNPs were found in 18 genes, and 14 of them were found in the exon region. Nonsynonymous coding SNPs were found...
Source: Biopreservation and Biobanking - April 3, 2019 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

The Australian and New Zealand Children's Haematology/Oncology Group Biobanking Network.
Authors: Byrne J, ANZCHOG Biobanking Network PMID: 30938543 [PubMed - as supplied by publisher] (Source: Biopreservation and Biobanking)
Source: Biopreservation and Biobanking - April 3, 2019 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Special Section: A Well-Preserved 2000-Year-Old Chinese Cadaver.
Authors: Yan XX, Chen D, Wang H, Wang XS, Luo XG PMID: 30920290 [PubMed - as supplied by publisher] (Source: Biopreservation and Biobanking)
Source: Biopreservation and Biobanking - March 30, 2019 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Autopsy and Forensic Study on a Rare Human Corpse Preserved Over Two Thousand Years: The Mawangdui Ancient Cadaver.
Authors: Chen D, Huang JF, Chen JM, You ZQ, Wang H, Wang XS, Yan XX, Luo XG Abstract In 1972, an enormous tomb site was found in the eastern suburb of Changsha, the capital city of Hunan Province, which led to the discovery of Mawangdui tomb No. 1, and soon thereafter tombs Nos. 2 and 3. These tombs were dated back to the Western Han Dynasty (206 BC-24 AD) in Chinese history. Along with numerous precious historic relics unearthed as grave goods, a well-preserved female cadaver was the most unprecedented, which was considered as one of the world's greatest archeological discoveries in the 20th century. The cadaver w...
Source: Biopreservation and Biobanking - March 30, 2019 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Reappraisal of the Mawangdui Han Tomb Cadaver Thirty Years After Its Unearthing.
Authors: Wang H, Chen JM, Wang XS, You ZQ, Chen D, Chen QL, Wu XY, Peng GC, Xia ZD, Tang HH, Yan XX, Huang JF, Luo XG Abstract The Mawangdui tomb No.1 cadaver, a female corpse from the Western Han Dynasty, was unearthed in 1972. Forensic examination at the time of discovery indicated fairly remarkable presence of bodily constituents at the anatomical, histological, and molecular levels. The cadaver was preserved in a formalin-based fixative afterward, and maintained in the Hunan Museum. To better protect this rare human corpse, a reappraisal of the status of preservation was carried out using noninvasive approaches...
Source: Biopreservation and Biobanking - March 30, 2019 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

The Coral Hospital.
Authors: Mayfield AB, Tsai S, Lin C Abstract Herein we propose an ambitious confrontation of the current coral reef crisis through the establishment of a "Coral Hospital." In an analogous manner to a human hospital, "sick" corals will first be diagnosed either in situ or in the hospital's diagnostic "clinic" such that the root cause of illness can be discerned (e.g., disease, high temperatures, or pollutant stress). Then, corals will be "treated" (when necessary) and allowed to "convalesce" in precisely controlled coral husbandry facilities. Upon "rehabilitatio...
Source: Biopreservation and Biobanking - March 27, 2019 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Real-Time Temperature Mapping in Ultra-Low Freezers as a Standard Quality Assessment.
Authors: Powell S, Molinolo A, Masmila E, Kaushal S Abstract Adequate preservation of biospecimens has been proven to be critical to obtain reliable and reproducible results in genomics, transcriptomics, proteomics, and many other assays. Most biological assays can be performed on specimens preserved in -80°C ultra-low freezers, but their quality can be influenced by temperature variability within storage units. Thus, regulatory standards such as those from the College of American Pathologists (CAP), the federal Clinical Laboratory Improvement Amendments, and standards from the Food and Drug Administration requ...
Source: Biopreservation and Biobanking - March 27, 2019 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

DNAshell Protects DNA Stored at Room Temperature for Downstream Next-Generation Sequencing Studies.
Authors: Washetine K, Heeke S, Ribeyre C, Bourreau C, Normand C, Blons H, Laurent-Puig P, Mulot C, Clermont D, David M, Clément B, Dagher G, Hofman P PMID: 30912674 [PubMed - as supplied by publisher] (Source: Biopreservation and Biobanking)
Source: Biopreservation and Biobanking - March 27, 2019 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Managing the Unimaginable: Biobank Participant Views on Reconsent for Whole Genome Sequencing of Stored Biospecimens.
DISCUSSION: Our findings highlight a potential discrepancy between the broad permissions granted by biobank participants at the time they donated biospecimens and their views about the application of WGS to their samples. Biobank participants appear to value the ability to confirm their commitment to genetic research when the studies in question involve WGS, a technological capacity they may not have anticipated at the time they donated their biospecimens. Efforts to reevaluate biobank participants' views about the acceptability of new technologies may help to ensure alignment of participants' current beliefs and research ...
Source: Biopreservation and Biobanking - March 27, 2019 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Advances and Challenges of Using Ovarian Preantral Follicles to Develop Biobanks of Wild Mammals.
Authors: Campos LB, Praxedes ÉCG, Saraiva MVA, Comizzoli P, Silva AR Abstract The extinction rate of mammalian species has been accelerated in recent decades. It therefore is important to preserve and store genetic materials in cryobanks for research purposes and subsequent production of offspring through assisted reproductive techniques. Along with the systematic collection and storage of germplasm, research efforts focusing on in vitro culture to produce mature gametes are critical. Specifically, obtaining mature oocytes from in vitro culture of preantral follicles is a great option to enhance female ferti...
Source: Biopreservation and Biobanking - March 23, 2019 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

A Two-Level Biobank Data Protection Concept for Project-Driven Human Sample Collections.
In this study, we present the data protection concept of our project driven, stand-alone biobank, focusing on meeting two biomaterial and data management areas simultaneously: operation of primary research projects involved in sample collection and long-term provision of biomaterial for future research purposes. The concept is based on national and international laws and ethical demands. Since the presented measures are transparent and basic, they should encourage biobanks in defining their own data protection concept and be easily transferable to different legal requirements. PMID: 30882231 [PubMed - as supplied by pu...
Source: Biopreservation and Biobanking - March 20, 2019 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Mawangdui-Type Ancient Human Cadavers in China and Strategies for Their Long-Term Preservation.
Authors: Wang XS, Chen D, Wang H, Liu L, Huang JF, Duan XM, Yan XX, Luo XG Abstract Ancient human remains may exist as intact cadavers in various forms, including mummies as well as humid or soft corpses. These valuable human depositories have been increasingly investigated with modern molecular biological approaches, delivering breakthrough discoveries in the field of paleoanthropology. Many ancient remains are also preserved in museums for public education of the history of human civilization. The Mawangdui tomb No. 1 cadaver was unearthed in 1972 in Changsha, China, and is a well-preserved humid-type corpse of a...
Source: Biopreservation and Biobanking - March 20, 2019 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Utilization of Archived Plasma to Detect Epidermal Growth Factor Receptor Mutation in Non-Small Cell Lung Cancer Patients.
In this study, we evaluated the epidermal growth factor receptor (EGFR) mutation status of samples of lung cancer patients stored before introduction of the plasma EGFR test at our institution. The aim of this study was to validate the utility of archived plasma samples for detection of the EGFR mutation in nonsmall cell lung cancer (NSCLC) patients. The Cobas® EGFR Mutation Test v2 was the first liquid biopsy test approved as a companion diagnostic test for patients with NSCLC treated with tyrosine kinase inhibitors. We tested for the EGFR mutation in 116 plasma samples archived in the biobank, and the results were co...
Source: Biopreservation and Biobanking - March 20, 2019 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

The ISBER 2019 Awards.
Authors: Hill K, Kozlakidis Z PMID: 30888849 [PubMed - as supplied by publisher] (Source: Biopreservation and Biobanking)
Source: Biopreservation and Biobanking - March 20, 2019 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Effect of Cryopreservation on Lipid Peroxidation, Antioxidant Potential, Chromatin Integrity, and Mitochondrial Activity of Indian Red Jungle Fowl (Gallus gallus murghi) Semen.
Authors: Ansari MS, Rakha BA, Akhter S, Blesbois E, Santiago-Moreno J Abstract AIM: The study elucidates the impact of cryopreservation on lipid peroxidation (LPO), antioxidant potential, DNA integrity, and mitochondrial activity of Indian red jungle fowl sperm. MATERIALS AND METHODS: Semen from eight mature cocks was pooled and diluted at 37°C using a specific medium for red fowl species. The diluted semen samples were immediately refrigerated at 4°C for 2 hours (0.275°C min-1). Glycerol was then added to a final concentration of 20%, and the samples were equilibrated for 10 minutes at 5°C befo...
Source: Biopreservation and Biobanking - February 24, 2019 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

RNA and DNA Integrity Remain Stable in Frozen Tissue After Long-Term Storage at Cryogenic Temperatures: A Report from the Ontario Tumour Bank.
CONCLUSIONS: Extended cryogenic storage beyond 2-11 years remains a viable option for maintaining the high quality of specimens in biobanks. PMID: 30762427 [PubMed - as supplied by publisher] (Source: Biopreservation and Biobanking)
Source: Biopreservation and Biobanking - February 16, 2019 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Biobanking and Beyond: The Importance of Scientific Collections.
Authors: Vaught J PMID: 30759029 [PubMed - in process] (Source: Biopreservation and Biobanking)
Source: Biopreservation and Biobanking - February 14, 2019 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Effect of Different Extenders and Dilution Ratios on the Spermatological and Fertilization Parameters of Cyprinus carpio During Cryopreservation.
Authors: Betsy CJ, Kumar JSS, Jawahar KTP Abstract Production of quality seeds is a critical need, and cryopreservation serves as an important biotechnological tool in achieving that goal. In the present study, the effects of different extenders and dilution ratios on the spermatological and fertilization parameters of cryopreserved milt were evaluated in common carp brooders. Milt was collected from Cyprinus carpio brooders and diluted and cryopreserved at three dilution ratios such as 1:40, 1:80, and 1:120, using Freshwater Fish Saline (FWFS) and Modified Fish Ringer as extenders and dimethyl sulfoxide as cryopro...
Source: Biopreservation and Biobanking - February 13, 2019 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Next Chapter of the Taiwan Biobank: Sustainability and Perspectives.
Authors: Lin JC, Chen LK, Hsiao WW, Fan CT, Ko ML Abstract The emergence of biobanks has expanded the scope of biomedical research, ushering in an era of "precision medicine" to improve the treatment of disease. However, biobanks also face sustainability challenges comprising three dimensions-"financial," "operational," and "social." The Taiwan Biobank (TWB), as a large-scale national biobank that supplies valuable phenotypic and genetic information to biomedical researchers on an application basis to investigate the relationship among personal health, genes, lifestyle, envir...
Source: Biopreservation and Biobanking - February 6, 2019 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Off-the-Shelf Cell Sheets as a Pleural Substitute for Closing Visceral Pleural Injuries.
Authors: Kanzaki M, Takagi R, Isaka T, Yamato M Abstract During pulmonary resections, removal of visceral pleura is frequently required, resulting in lung air leakage (LAL) and bleeding. Especially persistent LAL after pulmonary surgery has negative consequences. Current surgical procedures are ineffective in closing these visceral pleural injuries. Previously, the authors' laboratory has developed a novel and effective LAL sealant using tissue-engineered cell sheets harvested from temperature-responsive culture dishes. The clinical application of fresh fibroblast sheets (FSs) is limited by several problems related...
Source: Biopreservation and Biobanking - February 3, 2019 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

ISBER's Global Outlook: A Summary of Recent International Activities.
Authors: Catchpoole DR, Parry-Jones A, Kozlakidis Z PMID: 30694698 [PubMed - as supplied by publisher] (Source: Biopreservation and Biobanking)
Source: Biopreservation and Biobanking - January 31, 2019 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Dietary Fish Oil and Vitamin E Reduce Cryoinjuries and Apoptosis in Vitrified Laying Hens' Ovarian Tissue.
Authors: Nateghi R, Alizadeh Moghadam Masouleh A, Jafari Ahangari Y, Fathi R, Akhlaghi A Abstract The occurrence of cryoinjuries during ovarian tissue freezing necessitates development of methods that can overcome these challenges in cell and tissue cryopreservation. It has been hypothesized that omega-3 and vitamin E diet supplementation to the animal could be an appropriate strategy to preserve post-thaw ovarian quality. The laying hen is an appropriate animal model that can be used to study the effects of providing dietary supplements on the outcomes of ovary cryopreservation. The purpose of the study was to eva...
Source: Biopreservation and Biobanking - January 25, 2019 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Changing the Culture of Tumor Tissue Biobanking in a Tertiary Referral Hospital Using an Audit and Feedback Strategy.
We examined whether the introduction of an audit and feedback strategy applied to specimens dissected at the John Hunter Hospital anatomical pathology department improved the number of formalin fixed tumor specimens sampled by anatomical pathology registrars for the Hunter Cancer Biobank. During the audit period (1/7/16 to 30/6/17) a total of 949 tumor specimens were sampled for the biobank compared with 393 of the previous year (1/7/15 to 30/6/16) resulting in a 141% increase in specimens biobanked. A targeted group of previously underrepresented specimen types, including brain, lung, and lymph node tumors were studied in...
Source: Biopreservation and Biobanking - January 15, 2019 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Critical Financial Challenges for Biobanking: Report of a National Cancer Institute Study.
CONCLUSIONS: The survey results highlight several issues that are important for long-term biobank sustainability. It is critical to prepare for such issues as effective biobanking practices have increasingly been recognized as a key component for the advancement of precision medicine. PMID: 30638412 [PubMed - as supplied by publisher] (Source: Biopreservation and Biobanking)
Source: Biopreservation and Biobanking - January 15, 2019 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Addition of Equex STM to Extender Improves Post-Thawing Longevity of Collared Peccaries' Sperm.
Authors: Bezerra LGP, Souza ALP, Lago AEA, Campos LB, Nunes TL, Paula VV, Oliveira MF, Silva AR Abstract The effect of Equex STM® paste supplementation on the Tris-extender for collared peccaries' semen cryopreservation was assessed. Semen from 12 mature individuals was obtained by electroejaculation and evaluated for morphology, membrane integrity, osmotic response, and sperm kinetic metrics. Samples were diluted in Tris plus 20% egg yolk and divided into three aliquots. The first aliquot was without any supplementation, the second and third contained 0.5 and 1.0% Equex STM, respectively. The samples were adde...
Source: Biopreservation and Biobanking - January 4, 2019 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research

Semen Cryopreservation and Banking for the Conservation of Neotropical Carnivores.
Authors: Silva HVR, Silva AR, da Silvada LDM, Comizzoli P Abstract Neotropical carnivores include a large number of threatened and endangered species. It is critical to develop conservation efforts to ensure the sustainability of populations in situ and ex situ. The highest priorities are to protect natural habitats and better understand the biology of rare species. Conservation efforts also are directed toward the implementation of breeding programs and the development of reproductive biotechnologies in which the cryopreservation of male gametes plays a major role. It also is fundamental to create semen banks that...
Source: Biopreservation and Biobanking - December 29, 2018 Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research