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Perineural invasion in early-stage cervical cancer and its relevance following surgery.
Authors: Zhu Y, Zhang G, Yang Y, Cui L, Jia S, Shi Y, Song S, Xu S Abstract Perineural invasion (PNI) is the neoplastic invasion of nerves by cancer cells, a process that has attracted attention as a novel prognostic factor for cancer. The present study aimed to investigate the prognostic value of PNI in patients with early-stage cervical cancer (International Federation of Gynecology and Obstetrics stage IA2-IIA). A total of 210 patients who underwent radical hysterectomy and pelvic lymphadenectomy between 2007 and 2012 were included in the current study, of whom 8.57% (18/210) exhibited PNI. Patients with PNI wer...
Source: Oncology Letters - May 15, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Long non-coding RNA LSINCT5 promotes ovarian cancer cell proliferation, migration and invasion by disrupting the CXCL12/CXCR4 signalling axis.
Authors: Long X, Li L, Zhou Q, Wang H, Zou D, Wang D, Lou M, Nian W Abstract Long stress-induced noncoding transcript 5 (LSINCT5) is a member of the LSINCT family, members of which are expressed during stress-induced cell formation and have also been reported to promote cancer progression. In the present study, the association between LSINCT5 expression and clinical significance was investigated and the biological function of LSINCT5 in epithelial ovarian cancer (EOC) was explored. LSINCT5 expression was examined in EOC tissues by reverse transcription-quantitative polymerase chain reaction and its association with...
Source: Oncology Letters - May 15, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Phytochemicals: Current strategies for treating breast cancer.
Authors: Israel BB, Tilghman SL, Parker-Lemieux K, Payton-Stewart F Abstract Females with early-stage metastatic, estrogen-dependent breast cancer are generally treated with surgery, radiation and chemotherapy, or with more targeted approaches such as aromatase inhibitors (anastrozole or letrozole) or anti-estrogens (tamoxifen). Despite widespread successful usage of these agents for the treatment of breast cancer, resistance, tumor relapse and metastasis remain the principal causes of mortality for patients with breast cancer. While numerous groups have made major contributions toward an improved understanding of ...
Source: Oncology Letters - May 15, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

5-Fluorouracil may enrich cancer stem cells in canine mammary tumor cells in vitro.
In conclusion, stimulation with chemotherapeutic drugs including 5-FU is a good method for increasing the proportion of canine mammary tumor stem cells in vitro, which may provide further understanding of chemotherapeutic methods and CSCs. PMID: 29755597 [PubMed] (Source: Oncology Letters)
Source: Oncology Letters - May 15, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Long noncoding AFAP1-antisense RNA 1 is upregulated and promotes tumorigenesis in gastric cancer.
In conclusion, the results of the present study suggested that AFAP1-AS1 may serve as a valuable prognostic indicator and therapeutic target for GC. PMID: 29740481 [PubMed] (Source: Oncology Letters)
Source: Oncology Letters - May 11, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Dihydroartemisinin sensitizes Lewis lung carcinoma cells to carboplatin therapy via p38 mitogen-activated protein kinase activation.
Authors: Zhang B, Zhang Z, Wang J, Yang B, Zhao Y, Rao Z, Gao J Abstract Dihydroartemisinin (DHA), a semi-synthetic derivative of artemisinin isolated from the traditional Chinese herb Artemisia annua, is an effective novel antimalarial agent. Studies have suggested that it also exhibits anticancer effects when administered alone or in combination with conventional chemotherapeutic agents. The present study investigated the therapeutic effect of DHA combined with carboplatin (CBP) on Lewis lung carcinoma (LLC) cells and the possible underlying molecular mechanisms. MTT and clonogenic assays demonstrated that the pr...
Source: Oncology Letters - May 11, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Reduced miR-433 expression is associated with advanced stages and early relapse of colorectal cancer and restored miR-433 expression suppresses the migration, invasion and proliferation of tumor cells in vitro and in nude mice.
In conclusion, miR-433 may be a putative tumor suppressor in colorectal cancer, and the detection of low miR-433 expression will be investigated in further studies as a putative biomarker for the detection of early relapse in patients with colorectal cancer. PMID: 29740483 [PubMed] (Source: Oncology Letters)
Source: Oncology Letters - May 11, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Establishment of a GIST-T1 gastrointestinal stromal tumour cell line resistant to imatinib mesylate.
Authors: Zhou Y, Chen J, Weng X, Lin G, Huang Z, Shui H Abstract In the present study, imatinib mesylate (IM) was used to induce resistance in the gastrointestinal stromal tumour (GIST) cell line, GIST-T1, to establish a stable resistant cell line. The growth characteristics and expression profile of the established cell line were compared with those of the parental cell line. Additionally, the resistance mechanism of the gastrointestinal stromal tumours was preliminarily investigated. The GIST-T1 cells were cultured in vitro, and the drug was administered in the logarithmic phase of cell growth using intermittent ...
Source: Oncology Letters - May 11, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Effects of dexamethasone on C6 cell proliferation, migration and invasion through the upregulation of AQP1.
Authors: Guan Y, Chen J, Zhan Y, Lu H Abstract Dexamethasone (Dex) is commonly used to treat glioma; however, the mechanism underlying the action of Dex remains unclear. In the present study, the hypothesis that aquaporin-1 (AQP1) may participate in tumor cell proliferation, apoptosis, migration and invasion was tested using small interfering RNA (siRNA). The results of the current study indicated that Dex could inhibit the proliferation, in addition to promoting the migration, of C6 cells. Dex was indicated to promote the expression of AQP1. Downregulation of AQP1, achieved using siRNAs, demonstrated the inhibitio...
Source: Oncology Letters - May 11, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Genome-wide identification of epithelial-mesenchymal transition-associated microRNAs reveals novel targets for glioblastoma therapy.
Authors: Zhang Y, Zeng A, Liu S, Li R, Wang X, Yan W, Li H, You Y Abstract MicroRNAs (miRNA) regulate a number of cellular processes. Recent studies have indicated that these molecules function in the epithelial-mesenchymal transition (EMT). However, the crucial systematic role of EMT and miRNAs together in glioblastoma (GBM) remains poorly understood. The present study demonstrated that EMT was closely associated with malignant progression and clinical outcome using three independent glioma databases (GSE16011, Rembrandt and The Cancer Genome Atlas). Furthermore, integrated analysis of miRNAs and mRNA profiling in...
Source: Oncology Letters - May 11, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Label-free quantitative proteomic analysis identifies CTNNB1 as a direct target of FOXP3 in gastric cancer cells.
Authors: Pan DY, Zeng XQ, Ma GF, Gao J, Li N, Miao Q, Lian JJ, Zhou H, Xu LL, Chen SY Abstract Forkhead box protein 3 (FOXP3) is expressed in numerous types of tumor cell and is associated with tumor progression and prognosis. A previous study reported that FOXP3 inhibited cellular proliferation and induced apoptosis of gastric cancer (GC) cells by activating the apoptosis signaling pathway. In the present study, label-free quantitative proteomic analysis and chromatin immunoprecipitation-polymerase chain reaction (ChIP-PCR) was performed to investigate the mechanism by which the anticancer role of FOXP3 was mediat...
Source: Oncology Letters - May 11, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Imaging performance and clinical value of contrast-enhanced ultrasonography and computed tomography in the diagnosis of liver cancer.
Authors: Sun B, Lv Y, Xing D, Li J Abstract The imaging performance and clinical value of contrast-enhanced ultrasonography (CEUS) combined with CT in diagnosis of liver cancer were investigated. A total of 426 liver cancer patients treated in Yuhuangding Hospital (Yantai, China) from February 2011 to May 2016 were selected. Among them, 222 patients underwent CEUS examination, 204 patients underwent CT examination, and 102 patients underwent CEUS combined with CT examination. Sensitivity and specificity of the three methods were examined. CT showed a low density in 92.6% of patients (189 patients) and a high-low hy...
Source: Oncology Letters - May 11, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Dedifferentiated liposarcoma of the oral floor: A case study and literature review of 50 cases of head and neck neoplasm.
Authors: Nimura F, Nakasone T, Matsumoto H, Maruyama T, Matayoshi A, Maruyama N, Yoshimi N, Arasaki A, Nishihara K Abstract Dedifferentiated liposarcoma (DDLS) has a relatively poor prognosis, however this neoplasm rarely occurs in the head and neck. To date, no definite protocol has been established for the diagnosis and treatment of head and neck DDLS. The present study reports the case of a 69-year-old male patient with DDLS of the oral floor. To the best of our knowledge, this is the first documented case of oral floor DDLS. In addition, this is the first reported case with the development of a second primary m...
Source: Oncology Letters - May 11, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Silencing Livin improved the sensitivity of colon cancer cells to 5-fluorouracil by regulating crosstalk between apoptosis and autophagy.
Authors: Liu S, Li X, Li Q, Liu H, Shi Y, Zhuo H, Li C, Zhu H Abstract Colorectal cancer (CRC) is the third most common cause of cancer-associated mortality worldwide. Currently, 5-fluorouracil (5-FU) remains a widely used chemotherapeutic drug in the treatment of CRC; however, 5-FU resistance during treatment has become a common problem. Livin, a member of the inhibitor of apoptosis protein family, is considered to be associated with tumor resistance to chemotherapy. In the present study, Livin-silenced cells were generated by introducing a lentivirus into HCT116 and SW620 colon cancer cell lines. Acridine orange/...
Source: Oncology Letters - May 11, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Aberrant microRNA-137 promoter methylation is associated with lymph node metastasis and poor clinical outcomes in non-small cell lung cancer.
In conclusion, the results of the present study indicated that miR-137 is downregulated and that its promoter is aberrantly methylated in lung cancer, and that high levels of miR-137 promoter methylation may have prognostic value for poor disease-free survival. PMID: 29740491 [PubMed] (Source: Oncology Letters)
Source: Oncology Letters - May 11, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Downregulation of Epstein-Barr virus-induced gene 3 is associated with poor prognosis of hepatocellular carcinoma after curative resection.
Authors: Song Q, Chen X, Hu W, Mei G, Yang X, Wu H Abstract Epstein-Barr virus-induced gene 3 (EBI3) encodes a secretory glycoprotein, and has previously been identified as upregulated in various types of tumors. The presnet study examined the clinical significance of EBI3 expression for predicting tumor recurrence and survival after resection in hepatocellular carcinoma (HCC). EBI3 expression in various HCC cell lines and in 20 pairs of tumor and peritumor tissue samples were detected using western blot analysis. Immunohistochemical staining using tissue microarray with 312 samples from randomly selected patients ...
Source: Oncology Letters - May 11, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Bioinformatic analysis and prediction of the function and regulatory network of long non-coding RNAs in hepatocellular carcinoma.
Authors: Cao MR, Han ZP, Liu JM, Li YG, Lv YB, Zhou JB, He JH Abstract Computational analysis and bioinformatics have significantly advanced the ability of researchers to process and analyze biological data. Molecular data from human and model organisms may facilitate drug target validation and identification of biomarkers with increased predictive accuracy. The aim of the present study was to investigate the function of long non-coding RNAs (lncRNAs) in hepatocellular carcinoma (HCC) using online databases, and to predict their regulatory mechanism. HCC-associated lncRNAs, their downstream transcription factors an...
Source: Oncology Letters - May 11, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Addition of bevacizumab to systemic therapy for locally advanced and metastatic nasopharyngeal carcinoma.
Authors: Zhang HJ, Yuan GL, Liang QL, Peng XX, Cheng SA, Jiang L Abstract Radiotherapy is a vital treatment option for patients with nasopharyngeal carcinoma (NPC). Concurrent cisplatin-based radiochemotherapy with or without adjuvant chemotherapy had acquired good clinical effects with good local control rates. However, a number of patients present with metastasis following systemic regimens or initial diagnosis of locally advanced NPC, which cause difficulty for subsequent therapy. Therefore, there is an urgent requirement to discover novel targeted therapies. The present report describes one case of a patient wi...
Source: Oncology Letters - May 11, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

In vitro assessment of the role of DpC in the treatment of head and neck squamous cell carcinoma.
Authors: Xu YX, Zeng ML, Yu D, Ren J, Li F, Zheng A, Wang YP, Chen C, Tao ZZ Abstract The present study aimed to investigate the antitumor efficacy of di-2-pyridylketone-4-cyclohexyl-4-methyl-3-thiosemicarbazone (DpC) and di-2-pyridylketone-4,4,-dimethyl-3-thiosemicarbazone (Dp44mT) on head and neck squamous cell carcinoma (HNSCC) cells. The proliferation and apoptosis of HNSCC cells treated with the iron chelators DpC and Dp44mT were detected. The mechanism of DpC-induced apoptosis on HNSCC cells was investigated. The human HNSCC cell lines FaDu, Cal-27 and SCC-9 were cultured in vitro and exposed to gradient conc...
Source: Oncology Letters - May 11, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Pyrophosphatase 1 expression is associated with future recurrence and overall survival in Chinese patients with intrahepatic cholangiocarcinoma.
Authors: Xu D, Miao Y, Gu X, Wang J, Yu G Abstract The inorganic pyrophosphatase gene (PPA1) encodes inorganic pyrophosphatase, an enzyme that catalyzes the hydrolysis of inorganic pyrophosphate to orthophosphate, and has been revealed to be dysregulated in several types of human cancer. However, the role of PPA1 in intrahepatic cholangiocarcinoma (ICC) has not yet been determined. The present study detected PPA1 expression and investigated its clinical significance in ICC. Tissue microarray blocks containing 93 ICC specimens were constructed. The protein expression of PPA1 in these specimens was detected by immuno...
Source: Oncology Letters - May 11, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Clinical outcomes and associated factors of radioiodine-131 treatment in differentiated thyroid cancer with cervical lymph node metastasis.
Authors: Cao CJ, Dou CY, Lian J, Luan ZS, Zhou W, Xie W, Chen L, Zhou K, Lai H Abstract Cervical lymph node metastasis (CLNM) is common in differentiated thyroid cancer (DTC). Radioiodine-131 (131I) treatment is recommended for the removal of residual thyroid tissue following thyroidectomy. To date, the effect of 131I therapy on the outcomes of patients with DTC with CLNM is unclear. The aim of the present study was to evaluate the final outcome of patients with DTC with CLNM according to 131I administration, and to analyze the factors that may affect clinical outcomes. A total of 357 patients with DTC with CLNM we...
Source: Oncology Letters - May 11, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

MicroRNA-493 inhibits the proliferation and invasion of osteosarcoma cells through directly targeting specificity protein 1.
Authors: Qian M, Gong H, Yang X, Zhao J, Yan W, Lou Y, Peng D, Li Z, Xiao J Abstract Osteosarcoma (OS) is the most common type of primary bone tumor and accounts for ~60% of all malignant bone tumors in children and adolescents. A large number of studies have proposed that the dysregulated and dysfunctional microRNAs may serve important roles in the occurrence, progression and metastasis of various types of human cancer, including OS. MicroRNA-493 (miR-493) has been identified to act as a tumor suppressor in several types of human cancer. However, little is known regarding the expression pattern and clinical signif...
Source: Oncology Letters - May 11, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

The potential function of microRNAs as biomarkers and therapeutic targets in multiple myeloma.
Authors: Zhu B, Ju S, Chu H, Shen X, Zhang Y, Luo X, Cong H Abstract Multiple myeloma (MM), accounting for ~1% of all types of human cancer and 13% of all hematological malignancies, is characterized by the malignant proliferation of monoclonal plasma cells (PCs) in the bone marrow. MM leads to end stage organ impairment, including bone lesions, renal dysfunction, hypercalcemia and anemia. So far, the specific pathogenesis of MM remains unclear and no early-stage sensitive biomarker of MM has been well characterized. Furthermore, treating MM is difficult, as the majority of patients eventually relapse or become ref...
Source: Oncology Letters - May 10, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Effect of RAD51C expression on the chemosensitivity of E μ-Myc p19Arf-/- cells and its clinical significance in breast cancer.
Effect of RAD51C expression on the chemosensitivity of Eμ-Myc p19Arf-/- cells and its clinical significance in breast cancer. Oncol Lett. 2018 May;15(5):6107-6114 Authors: Liao SG, Liu L, Wang YJ Abstract The aim of the present study was to investigate the chemosensitivity to anti-cancer drugs of RAD51 paralog C (RAD51C)-deficient Eμ-Myc p19Arf-/- cells, to detect the expression of RAD51C in breast cancer tissues by immunohistochemistry (IHC), and to explore their association with clinicopathological factors. Eμ-Myc p19Arf-/- cells were stably transfected with retroviruses co-expressing short ...
Source: Oncology Letters - May 10, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Antitumor effects of brucine immuno-nanoparticles on hepatocellular carcinoma in vivo.
Authors: Qin J, Yang L, Sheng X, Sa Z, Huang T, Li Q, Gao K, Chen Q, Ma J, Shen H Abstract In vitro and in vivo studies have demonstrated that brucine is able to inhibit the proliferation of liver cancer cells and growth of animal tumors, and may be a promising anticancer drug. However, high toxicity, poor water solubility, short half-life, narrow therapeutic window, and similar therapeutic and toxic doses limit its clinical application in the treatment of malignant tumors. In our previous study, brucine immuno-nanoparticles were successfully prepared and added to the culture medium of liver cancer SMMC-7721 cells,...
Source: Oncology Letters - May 10, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Combining the mammalian target of rapamycin inhibitor, rapamycin, with resveratrol has a synergistic effect in multiple myeloma.
Authors: Jin HG, Wu GZ, Wu GH, Bao YG Abstract Rapamycin is known to inhibit the mammalian target of rapamycin complex (mTORC)1 signaling pathway, but it is unable to effectively inhibit mTORC2, resulting in activation of protein kinase B in multiple myeloma (MM) cell lines. Additionally, certain studies have suggested that resveratrol has an effect on human MM cells, and that rapamycin in combination with resveratrol may be useful in cancer therapy. The present study aimed to investigate the combined treatment effect of resveratrol and rapamycin on the MM MM1.S cell line. The results demonstrated that combined tre...
Source: Oncology Letters - May 10, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Analysis of human MutS homolog 2 missense mutations in patients with colorectal cancer.
Authors: Zhang X, Chen S, Yu J, Zhang Y, Lv M, Zhu M Abstract Germline mutations of DNA mismatch repair gene human MutS homolog 2 (hMSH2) are associated with hereditary nonpolyposis colorectal cancer (HNPCC). A total of one-third of these mutations are missense mutations. Several hMSH2 missense mutations have been identified in patients in East Asia, although their function has not been evaluated. In the present study, the role of ten hMSH2 missense mutations in the pathogenesis of colorectal cancer was examined. The hMSH2/hMSH6 protein interaction system was established using yeast two-hybrid screening. Next, the ...
Source: Oncology Letters - May 10, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

The clinical value of detecting circulating tumour cells in the peripheral blood of nasopharyngeal carcinoma patients.
Authors: Chen Z, Xu L, Xu X, Yuan C Abstract The aim of the present study as to analyse the associations between circulating tumour cells (CTCs) and the clinical parameters of nasopharyngeal carcinoma (NPC). Peripheral blood (7.5 ml) from 68 first-diagnosed NPC patients was collected to detect and identify CTCs by cluster of differentiation (CD)45 immunomagnetic separation. Immunofluorescent staining of cytokeratin-18, CD45 and DAPI, and fluorescence in situ hybridization were combined with the centromere of chromosome 8 (CEP8) probe method to analyse the associations between CTCs and clinical parameters. One-year ...
Source: Oncology Letters - May 10, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Blue light-induced apoptosis of human promyelocytic leukemia cells via the mitochondrial-mediated signaling pathway.
Authors: Zhuang J, Liu Y, Yuan Q, Liu J, Liu Y, Li H, Wang D Abstract Acute promyelocytic leukemia is frequently associated with dizziness, fever, nausea, hematochezia and anemia. Blue light, or light with wavelengths of 400-480 nm, transmits high levels of energy. The aim of the present study was to determine the pro-apoptotic effects of blue light (wavelength, 456 nm; radiation power, 0.25 mW/cm2) and the underlying mechanisms in a human promyelocytic leukemia cell line (HL60). Blue light reduced the viability and enhanced the mortality of HL60 cells in a time-dependent manner. Exposure to blue light for 24 h cau...
Source: Oncology Letters - May 10, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Solamargine derived from Solanum nigrum induces apoptosis of human cholangiocarcinoma QBC939 cells.
Authors: Zhang X, Yan Z, Xu T, An Z, Chen W, Wang X, Huang M, Zhu F Abstract Solamargine, an active ingredient of Solanum nigrum, has been previously revealed to inhibit the proliferation of cancer cells. However, the effect of solamargine on human cholangiocarcinoma cells and the underlying molecular mechanism remain unknown. In the present study, the molecular mechanism underlying the anti-cancer effect of solamargine was assessed in human cholangiocarcinoma QBC939 cells. The results of the present study revealed that solamargine inhibited the viability of QBC939 cells in a dose-dependent manner. Furthermore, sol...
Source: Oncology Letters - May 10, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

miR-130b-5p promotes proliferation, migration and invasion of gastric cancer cells via targeting RASAL1.
Authors: Chen H, Yang Y, Wang J, Shen D, Zhao J, Yu Q Abstract The aim of the present study was to investigate the targeted interaction between microRNA (miR)-130b-5p and RAS protein activator like 1 (RASAL1) gene and elucidate the function of miR-130b-5p in cell proliferation, migration and invasion in gastric cancer. Expression of miR-130b-5p and RASAL1 in seven gastric cell lines was detected by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). MGC803 cells were selected for further study since they exhibited a marked increase in expression of miR-130b-5p accompanied by decreased expression...
Source: Oncology Letters - May 10, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Single-center analysis of appendiceal neoplasms.
In conclusion, the incidence of appendiceal neoplasms has increased in recent years, potentially due to increased and earlier detection by newer imaging modalities. PMID: 29731850 [PubMed] (Source: Oncology Letters)
Source: Oncology Letters - May 10, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

The family of apoptosis-stimulating proteins of p53 is dysregulated in colorectal cancer patients.
Authors: Yin L, Lin Y, Wang X, Su Y, Hu H, Li C, Wang L, Jiang Y Abstract The apoptosis-stimulating protein of p53 (ASPP) family is a newly identified family protein including ASPP1, ASPP2 and inhibitor of ASPP (iASPP), by which the tumor protein 53 (TP53)-mediated apoptotic process is selectively regulated. Downregulation of ASPP1/ASPP2 and upregulation of iASPP were revealed to be associated with a poor prognosis and metastasis in several types of cancer. However, to the best of our knowledge, the expression of ASPP in colorectal cancer (CRC) has not previously been investigated. The present study analyzed ASPP e...
Source: Oncology Letters - May 10, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Identification of potential diagnostic and prognostic biomarkers in non-small cell lung cancer based on microarray data.
Authors: Huang R, Gao L Abstract Non-small cell lung cancer (NSCLC) is the most commonly diagnosed subtype of lung cancer, and the leading cause of cancer-associated mortalities worldwide. However, NSCLC is typically diagnosed at a late stage of disease due to a lack of effective diagnostic methods. In the present study, the GSE19804 dataset was obtained from the Gene Expression Omnibus, and a number of differentially expressed genes were identified between NSCLC and adjacent normal tissues. Based on functional and pathway enrichment analyses, five hub genes (cell-division cycle 20, centromere protein F, kinesin fa...
Source: Oncology Letters - May 10, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Targeted gene therapy of the HSV-TK/hIL-12 fusion gene controlled by the hSLPI gene promoter of human non-small cell lung cancer in vitro.
Authors: Hao S, Du X, Song Y, Ren M, Yang Q, Wang A, Wang Q, Zhao H, Du Z, Zhang G Abstract The incidence of lung cancer and lung cancer-associated mortality have markedly increased worldwide, and gene-targeted therapy has emerged as a promising treatment strategy. The present study aimed to explore the targeted antitumor effect of the herpes simplex virus-thymidine kinase/human interleukin-12 (HSV-TK/hIL-12) fusion gene regulated by the human secretory leukocyte protease inhibitor (hSLPI) promoter of human non-small cell lung cancer (hNSCLC). There were four recombinant eukaryotic expression vectors: pcDNA3.1-CMV-...
Source: Oncology Letters - May 10, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Cantharidin suppresses cell growth and migration, and activates autophagy in human non-small cell lung cancer cells.
In conclusion, the results of the present study indicated that CTD impeded cell growth and migration by inhibiting PI3K/Akt/mTOR signaling in NSCLC, and promoted autophagy and apoptosis. CTD exhibited anticancer activity against NSCLC in vitro, revealing it as a potential candidate for the treatment of NSCLC. PMID: 29731854 [PubMed] (Source: Oncology Letters)
Source: Oncology Letters - May 10, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Auxiliary diagnostic value of p16 amplification combined with the detection of heterozygous and homozygous loss for urothelial carcinoma.
Authors: Mao X, Li B, Liang Y, Li S, Zhou J, He Q, Jiang N, Chen Y, Sun Y, Cui Y, Jiang W, Wang H, Wang L, Ke Z Abstract The present study aimed to investigate the significance of detecting cyclin dependent kinase inhibitor 2A (p16) gene aberrations in the diagnosis of urothelial carcinoma (UC) using fluorescence in situ hybridization (FISH). A total of 77 voided urine specimens from 65 patients with UC and 12 patients with benign urinary disease were recruited into the current study. Under a fluorescence microscope, cells with large and irregular nuclei were assessed for chromosomal aberrations. The positive rate ...
Source: Oncology Letters - May 10, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

p16 as a prognostic factor for the response to induction chemotherapy in advanced hypopharyngeal squamous cell carcinoma.
In this study, p16 was not determined to predict the chemotherapy response for HPSCC. High p16 expression did not correlate with survival incidence for patients with HPSCC. PMID: 29731856 [PubMed] (Source: Oncology Letters)
Source: Oncology Letters - May 10, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Effect of photodynamic therapy combined with Celecoxib on expression of cyclooxygenase-2 protein in HeLa cells.
Authors: Mao Y, Wang L, Xu C, Han S Abstract The present study assessed the effect of photodynamic therapy (PDT) combined with Celecoxib (Cel) on cervical cancer HeLa cells. An MTT assay was performed to detect the inhibitory effects of Cel with different concentrations (10, 50, 100, 150, 200, 250 and 300 µg/ml) on the proliferation of HeLa cells. Subsequently, HeLa cells were divided into control group (group H), 50 g/ml Celecoxib group (group C), PDT group (group P), 50 g/ml Cel + PDT group (group P + C) and western blotting and immunohistochemistry were performed to detect the expression of cyclooxygenase-...
Source: Oncology Letters - May 10, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Suppressive effects of lycopene and β-carotene on the viability of the human esophageal squamous carcinoma cell line EC109.
Suppressive effects of lycopene and β-carotene on the viability of the human esophageal squamous carcinoma cell line EC109. Oncol Lett. 2018 May;15(5):6727-6732 Authors: Ngoc NB, Lv P, Zhao WE Abstract The molecular mechanisms underlying the chemopreventive effects of carotenoids in different types of cancer are receiving increasing attention. In the present study, the role of peroxisome proliferator-activated receptor γ (PPARγ) in the effect of lycopene and β-carotene on the viability of EC109 human esophageal squamous carcinoma cells was investigated. The viability of EC109 cell...
Source: Oncology Letters - May 10, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Effects of DKK1 overexpression on bone metastasis of SBC-3 cells.
Authors: Pang H, Ma N, Shen W, Zhao Q, Wang J, Duan L, Chen W, Zhang N, Zhao Z, Liu L, Zhang H Abstract Among all malignancies, lung cancer is the leading cause of cancer-related deaths in China. Bone metastasis is one of the most common complications and one of the most important factors affecting the prognosis of lung cancer patients, which resulting in very poor therapeutic effects. Previously, we have demonstrated that the expression levels of Dickkopf1 (DKK1), a protein involved in cell regulation and proliferation, was dramatically higher in cells that have a tendency to metastasize and invade the bone tissue...
Source: Oncology Letters - May 10, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Utilization of lung cancer cell lines for the study of lung cancer stem cells.
Authors: Wang Y, Jiang M, Du C, Yu Y, Liu Y, Li M, Luo F Abstract Lung cancer is one of the most lethal types of cancer, and its poor prognosis is primarily due to drug resistance and cancer recurrence. As it is associated with a low five-year survival rate, lung cancer stem cells (LCSCs) have been the subject of numerous recent studies. For these studies of LCSCs, lung cancer cell lines are more commonly used than lung cancer tissues obtained from patients, as they are easier to acquire. The methods utilized for the identification of LCSCs from lung cancer cell lines include fluorescence activated cell sorting (FA...
Source: Oncology Letters - May 10, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Analysis of YAP1 and TAZ expression by immunohistochemical staining in malignant mesothelioma and reactive mesothelial cells.
Authors: Takehara Y, Yamochi T, Nagumo T, Cho T, Urushibara F, Ono K, Fujii T, Okamoto N, Sasaki Y, Tazawa S, Honma M, Norose T, Shiozawa E, Tate G, Takimoto M Abstract Gene mutations are involved in the development of malignant mesothelioma. Important mutations have been identified in the genes for cyclin-dependent kinase inhibitor 2A (p16) alternative reading frame, breast cancer-associated protein 1 (BAP1) and neurofibromatosis type 2 (NF2). Previously, the utility of detecting the loss of BAP1 by immunohistochemistry (IHC) and p16-deletion by fluorescence in situ hybridization has been identified in several stu...
Source: Oncology Letters - May 10, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Synergistic anti-tumor effects of dasatinib and dendritic cell vaccine on metastatic breast cancer in a mouse model.
Authors: Song N, Guo H, Ren J, Hao S, Wang X Abstract Immunotherapy is currently considered as one of the major anti-tumor modalities, but its efficacy is limited. Dasatinib could improve the expansion and recruitment of cluster of differentiation (CD) 8+T cells and natural killer (NK) cells to the tumor microenvironment. The present study aimed to evaluate the synergistic anti-tumor effects of dasatinib with dendritic cell (DC) vaccine in metastatic breast cancer. Dasatinib with DC vaccine was administered to mice inoculated with 4T1 breast cancer cells. Thereafter, tumor volume was measured every other day. On da...
Source: Oncology Letters - May 10, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

LIM and SH3 protein 1 knockdown suppresses proliferation and metastasis of colorectal carcinoma cells via inhibition of the mitogen-activated protein kinase signaling pathway.
Authors: Zhao H, Liu B, Li J Abstract LIM and SH3 protein 1 (Lasp-1), a focal adhesion protein, serves a critical role in the regulation of cell proliferation and migration. The role of Lasp-1, as well as the intracellular signaling pathways involved in metastasis and progression of colorectal carcinoma, remains unclear. In the present study, the regulatory effect of Lasp-1 and the underlying molecular mechanism involved in migration and invasion of colorectal carcinoma were investigated. RNA interference and overexpression in SW480 cells were performed to elucidate the role of Lasp-1. Reverse transcription-quantit...
Source: Oncology Letters - May 10, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

MicroRNA-542-3p suppresses tumor cell proliferation via targeting Smad2 inhuman osteosarcoma.
Authors: Wu Y, You J, Li F, Wang F, Wang Y Abstract Osteosarcoma (OS) is an aggressive malignant neoplasm that arises from primitive transformed cells of mesenchymal origin, and that exhibits osteoblastic differentiation and produces malignant osteoid. MicroRNA (miR)-542-3p has been reported to serve a crucial role in the initiation and development of several types of human cancer. However, whether miR-542-3p is involved in tumorigenesis and tumor progression of OS remains unclear. In the present study, the expression of miR-542-3p in OS cells and patients with OS, and its functional mechanism in OS were investigat...
Source: Oncology Letters - May 10, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Comparison of the algorithms classifying the ABC and GCB subtypes in diffuse large B-cell lymphoma.
Authors: Boltežar L, Prevodnik VK, Perme MP, Gašljević G, Novaković BJ Abstract Different immunohistochemical algorithms for the classification of the activated B-cell (ABC) and germinal center B-cell (GCB) subtypes of diffuse large B-cell lymphoma (DLBCL) are applied in different laboratories. In the present study, 127 patients with DLCBL were investigated, all treated with rituximab and cyclophosphamide, hydroxydaunorubicin, oncovin and prednisone (CHOP) or CHOP-like regimens between April 2004 and December 2010. Multi-tumor tissue microarrays were prepared and were tested according to 4 algorithms: Han...
Source: Oncology Letters - May 10, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

MicroRNA hsa-let-7e-5p as a potential prognosis marker for rectal carcinoma with liver metastases.
Authors: Chen W, Lin G, Yao Y, Chen J, Shui H, Yang Q, Wang X, Weng X, Sun L, Chen F, Yang S, Yang Y, Zhou Y Abstract MicroRNAs (miRNAs) are post-transcriptional regulators of gene expression that target mRNAs for translational repression or cleavage. The present study was conducted to identify differentially expressed miRNAs in primary tumor tissues of rectal carcinoma (RC) that may be associated with heterochrony hepatic metastasis (HHM). Samples were collected exclusively from patients with RC but not colon cancer (CC); Next-generation high-throughput sequencing technology and bioinformatics tools were used to p...
Source: Oncology Letters - May 10, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Combined application of Embelin and tumor necrosis factor-related apoptosis-inducing ligand inhibits proliferation and invasion in osteosarcoma cells via caspase-induced apoptosis.
Authors: Qian H, Chen Y, Huang T, Liu T, Li X, Jiang G, Zhang W, Cheng S, Li P Abstract Embelin, as an inhibitor of the X-linked inhibitor of apoptosis protein (XIAP), may induce apoptosis in various types of cancer cells. The present study aimed to determine the effect of Embelin on the tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis of osteosarcoma cells. Embelin and TRAIL were applied to U2OS and MG63 cells, respectively or in combination. MTT was initially used to detect the difference in survival rates between the group receiving combined application of 100 ng/ml TRAIL and 20 ...
Source: Oncology Letters - May 10, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

MicroRNA-192-5p suppresses the initiation and progression of osteosarcoma by targeting USP1.
Authors: Zhou S, Xiong M, Dai G, Yu L, Zhang Z, Chen J, Guo W Abstract Osteosarcoma is the most frequent primary malignant bone tumor. An increasing body of evidence has suggested that microRNAs (miRNA/miRs) have emerged as critical regulators in the initiation and progression of osteosarcoma. The present study explored the biological function of miR-192-5p and ubiquitin-specific protease 1 (USP1), and investigated whether miR-192-5p could directly interact with USP1 in osteosarcoma. The results revealed that miR-192-5p was significantly downregulated in osteosarcoma tissues and cell lines, while a reverse expressi...
Source: Oncology Letters - May 10, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research