Analysis of < em > RHOA < /em > mutations and their significance in the proliferation and transcriptome of digestive tract cancer cells
Oncol Lett. 2021 Oct;22(4):735. doi: 10.3892/ol.2021.12996. Epub 2021 Aug 11.ABSTRACTThe ras homolog family member A (RHOA) gene encodes a member of the Rho family of small GTPases and is known to function in reorganization of the actin cytoskeleton, which is associated with regulation of cell shape, attachment and motility. RHOA has been found to be recurrently mutated in gastrointestinal cancer; however, the functional significance of the mutated RHOA protein in digestive tract cancers remains to be uncovered. The aim of the present study was to understand the role of mutant RHOA in the proliferation and transcriptome of...
Source: Oncology Letters - September 1, 2021 Category: Cancer & Oncology Authors: Naoki Ikari Akiko Serizawa Etsuko Tanji Masakazu Yamamoto Toru Furukawa Source Type: research

TAGLN2 promotes the proliferation, invasion, migration and epithelial-mesenchymal transition of colorectal cancer cells by activating STAT3 signaling through ANXA2
Oncol Lett. 2021 Oct;22(4):737. doi: 10.3892/ol.2021.12998. Epub 2021 Aug 16.ABSTRACTColorectal cancer (CRC) is one of the leading causes of cancer-associated mortality worldwide and currently ranks third in the USA in terms of prevalence. Transgelin-2 (TAGLN2) was previously reported to serve as a tumor promoter in various types of cancer. The present study aimed to investigate the role of TAGLN2 in the progression of CRC and to determine the potential underlying mechanism. The expression level of TAGLN2 in CRC cells (HCT116, SNU-C1, LoVo and SW480) were first detected by reverse transcription quantitative PCR and western...
Source: Oncology Letters - September 1, 2021 Category: Cancer & Oncology Authors: Zhicheng Zhao Li Lu Weidong Li Source Type: research

Different properties of mammary carcinogenesis induced by two chemical carcinogens, DMBA and PhIP, in heterozygous BALB/c < em > Trp53 < /em > knockout mice
In conclusion, the present mouse mammary carcinogenesis models, induced by a combination of genetic and exogenous factors, may be utilized (such as the DMBA-induced model with Trp53 gene function deficiency as a model of adenomyoepithelioma, characterized by distinct biphasic cell constituents and Hras mutations), but PhIP-induced models are required to further analyze the genetic/epigenetic mechanisms promoting mammary carcinomas.PMID:34466150 | PMC:PMC8387855 | DOI:10.3892/ol.2021.12999 (Source: Oncology Letters)
Source: Oncology Letters - September 1, 2021 Category: Cancer & Oncology Authors: Yukino Machida Toshio Imai Source Type: research

Upregulation of microfibrillar-associated protein 2 is closely associated with tumor angiogenesis and poor prognosis in hepatocellular carcinoma
Oncol Lett. 2021 Oct;22(4):739. doi: 10.3892/ol.2021.13000. Epub 2021 Aug 16.ABSTRACTAbnormal expression of microfibrillar-associated protein 2 (MFAP2), a key regulator of cellular differentiation, affects the occurrence and progression of tumors. However, the underlying role of MAFP2 in hepatocellular carcinoma (HCC) remains unclear. In the present study, patterns of MFAP2 expression in HCC were analyzed using sequencing data from The Cancer Genome Atlas database. Expression profiles of MFAP2, as well as those of epithelial-mesenchymal transition (EMT)-related proteins, were compared between HCC pathological sections and ...
Source: Oncology Letters - September 1, 2021 Category: Cancer & Oncology Authors: Nu Zhang Feng Shao Weidong Jia Source Type: research

Lanatoside C inhibits human cervical cancer cell proliferation and induces cell apoptosis by a reduction of the JAK2/STAT6/SOCS2 signaling pathway
Oncol Lett. 2021 Oct;22(4):740. doi: 10.3892/ol.2021.13001. Epub 2021 Aug 17.ABSTRACTCervical cancer is one of the leading causes of cancer-associated mortality in gynecological diseases and ranks third among female cancers worldwide. Although early detection and vaccination have reduced incidence rates, cancer recurrence and metastasis lead to high mortality due to the lack of effective medicines. The present study aimed to identify novel drug candidates to treat cervical cancer. In the present study, lanatoside C, an FDA-approved cardiac glycoside used for the treatment of heart failure, was demonstrated to have anti-pro...
Source: Oncology Letters - September 1, 2021 Category: Cancer & Oncology Authors: Yingchun Duan Li Chen Juan Shao Cui Jiang Yingmei Zhao Yanyi Li Huihui Ke Rui Zhang Jianlong Zhu Minghua Yu Source Type: research

Erratum: MicroRNA-100 functions as a tumor suppressor in cervical cancer via downregulating the SATB1 expression and regulating AKT/mTOR signaling pathway and epithelial-to-mesenchymal transition
Oncol Lett. 2021 Oct;22(4):741. doi: 10.3892/ol.2021.13002. Epub 2021 Aug 17.ABSTRACT[This corrects the article DOI: 10.3892/ol.2020.11686.].PMID:34466153 | PMC:PMC8387881 | DOI:10.3892/ol.2021.13002 (Source: Oncology Letters)
Source: Oncology Letters - September 1, 2021 Category: Cancer & Oncology Authors: Cuiping Huang Xiaobo Qin Na Zhao Huijing Jin Shuangjun Zhang Haiyan Yang Source Type: research

Gold nanoparticles enhance microRNA 31 detection in colon cancer cells after inhibition with chlorogenic acid
Oncol Lett. 2021 Oct;22(4):742. doi: 10.3892/ol.2021.13003. Epub 2021 Aug 19.ABSTRACTIn the present study, the inhibitory effect of chlorogenic acid (CGA), a phenolic compound with potential antitumor effects, on circulating microRNA 31 (miR-31), was evaluated in RKO colon cancer cells. The capacity of gold nanoparticles (AuNPs) to enhance miR-31 quantification after treatment with CGA was assessed. RKO cells were treated with different concentrations of CGA for 24, 48 and 72 h, after which AuNPs coupled to CD81 were added to the supernatants. Total RNA was extracted, and miR-31 was quantified by reverse transcription-quan...
Source: Oncology Letters - September 1, 2021 Category: Cancer & Oncology Authors: Ana C Luque-Badillo Griselda Hernandez-Tapia Dhamar A Ramirez-Castillo Diego Espinoza-Serrano Alan M Cortes-Limon Juan P Cortes-Gallardo Daniel A Jacobo-Vel ázquez Margarita L Martinez-Fierro Clara P Rios-Ibarra Source Type: research

Pioglitazone mediates apoptosis in Caki cells via downregulating c-FLIP < sub > (L) < /sub > expression and reducing Bcl-2 protein stability
In conclusion, these findings indicated that pioglitazone-induced apoptosis is regulated through caspase-mediated degradation of FLIP(L) and reduction of Bcl-2 protein stability, suggesting that pioglitazone is a feasible apoptotic agent that could be used in the treatment of human RC.PMID:34466155 | PMC:PMC8387863 | DOI:10.3892/ol.2021.13004 (Source: Oncology Letters)
Source: Oncology Letters - September 1, 2021 Category: Cancer & Oncology Authors: Ji Hoon Jang Tae-Jin Lee Eon-Gi Sung In-Hwan Song Joo-Young Kim Source Type: research

Repositioning of duloxetine to target pancreatic stellate cells
The objective of the current study was to identify compounds that suppress PSC activation. Gene expression profiles of cancer-derived fibroblasts and normal fibroblasts were used, and the pathway analysis suggested altered pathways that were chosen for validation. It was found that the 'neuroactive ligand-receptor interaction' pathway from the Kyoto Encyclopedia of Genes and Genomes pathway analysis was one of the altered pathways. Several compounds related with this pathway were chosen, and changes in PSC activity were investigated using fluorescence staining of lipid droplets, reverse transcription-quantitative PCR, west...
Source: Oncology Letters - September 1, 2021 Category: Cancer & Oncology Authors: Akiko Sagara Kohei Nakata Sokichi Matsumoto Weiyu Guan Tomohiko Shinkawa Chika Iwamoto Naoki Ikenaga Kenoki Ohuchida Masafumi Nakamura Source Type: research

Analysis of < em > RHOA < /em > mutations and their significance in the proliferation and transcriptome of digestive tract cancer cells
Oncol Lett. 2021 Oct;22(4):735. doi: 10.3892/ol.2021.12996. Epub 2021 Aug 11.ABSTRACTThe ras homolog family member A (RHOA) gene encodes a member of the Rho family of small GTPases and is known to function in reorganization of the actin cytoskeleton, which is associated with regulation of cell shape, attachment and motility. RHOA has been found to be recurrently mutated in gastrointestinal cancer; however, the functional significance of the mutated RHOA protein in digestive tract cancers remains to be uncovered. The aim of the present study was to understand the role of mutant RHOA in the proliferation and transcriptome of...
Source: Oncology Letters - September 1, 2021 Category: Cancer & Oncology Authors: Naoki Ikari Akiko Serizawa Etsuko Tanji Masakazu Yamamoto Toru Furukawa Source Type: research

TAGLN2 promotes the proliferation, invasion, migration and epithelial-mesenchymal transition of colorectal cancer cells by activating STAT3 signaling through ANXA2
Oncol Lett. 2021 Oct;22(4):737. doi: 10.3892/ol.2021.12998. Epub 2021 Aug 16.ABSTRACTColorectal cancer (CRC) is one of the leading causes of cancer-associated mortality worldwide and currently ranks third in the USA in terms of prevalence. Transgelin-2 (TAGLN2) was previously reported to serve as a tumor promoter in various types of cancer. The present study aimed to investigate the role of TAGLN2 in the progression of CRC and to determine the potential underlying mechanism. The expression level of TAGLN2 in CRC cells (HCT116, SNU-C1, LoVo and SW480) were first detected by reverse transcription quantitative PCR and western...
Source: Oncology Letters - September 1, 2021 Category: Cancer & Oncology Authors: Zhicheng Zhao Li Lu Weidong Li Source Type: research

Different properties of mammary carcinogenesis induced by two chemical carcinogens, DMBA and PhIP, in heterozygous BALB/c < em > Trp53 < /em > knockout mice
In conclusion, the present mouse mammary carcinogenesis models, induced by a combination of genetic and exogenous factors, may be utilized (such as the DMBA-induced model with Trp53 gene function deficiency as a model of adenomyoepithelioma, characterized by distinct biphasic cell constituents and Hras mutations), but PhIP-induced models are required to further analyze the genetic/epigenetic mechanisms promoting mammary carcinomas.PMID:34466150 | PMC:PMC8387855 | DOI:10.3892/ol.2021.12999 (Source: Oncology Letters)
Source: Oncology Letters - September 1, 2021 Category: Cancer & Oncology Authors: Yukino Machida Toshio Imai Source Type: research

Upregulation of microfibrillar-associated protein 2 is closely associated with tumor angiogenesis and poor prognosis in hepatocellular carcinoma
Oncol Lett. 2021 Oct;22(4):739. doi: 10.3892/ol.2021.13000. Epub 2021 Aug 16.ABSTRACTAbnormal expression of microfibrillar-associated protein 2 (MFAP2), a key regulator of cellular differentiation, affects the occurrence and progression of tumors. However, the underlying role of MAFP2 in hepatocellular carcinoma (HCC) remains unclear. In the present study, patterns of MFAP2 expression in HCC were analyzed using sequencing data from The Cancer Genome Atlas database. Expression profiles of MFAP2, as well as those of epithelial-mesenchymal transition (EMT)-related proteins, were compared between HCC pathological sections and ...
Source: Oncology Letters - September 1, 2021 Category: Cancer & Oncology Authors: Nu Zhang Feng Shao Weidong Jia Source Type: research

Lanatoside C inhibits human cervical cancer cell proliferation and induces cell apoptosis by a reduction of the JAK2/STAT6/SOCS2 signaling pathway
Oncol Lett. 2021 Oct;22(4):740. doi: 10.3892/ol.2021.13001. Epub 2021 Aug 17.ABSTRACTCervical cancer is one of the leading causes of cancer-associated mortality in gynecological diseases and ranks third among female cancers worldwide. Although early detection and vaccination have reduced incidence rates, cancer recurrence and metastasis lead to high mortality due to the lack of effective medicines. The present study aimed to identify novel drug candidates to treat cervical cancer. In the present study, lanatoside C, an FDA-approved cardiac glycoside used for the treatment of heart failure, was demonstrated to have anti-pro...
Source: Oncology Letters - September 1, 2021 Category: Cancer & Oncology Authors: Yingchun Duan Li Chen Juan Shao Cui Jiang Yingmei Zhao Yanyi Li Huihui Ke Rui Zhang Jianlong Zhu Minghua Yu Source Type: research

Erratum: MicroRNA-100 functions as a tumor suppressor in cervical cancer via downregulating the SATB1 expression and regulating AKT/mTOR signaling pathway and epithelial-to-mesenchymal transition
Oncol Lett. 2021 Oct;22(4):741. doi: 10.3892/ol.2021.13002. Epub 2021 Aug 17.ABSTRACT[This corrects the article DOI: 10.3892/ol.2020.11686.].PMID:34466153 | PMC:PMC8387881 | DOI:10.3892/ol.2021.13002 (Source: Oncology Letters)
Source: Oncology Letters - September 1, 2021 Category: Cancer & Oncology Authors: Cuiping Huang Xiaobo Qin Na Zhao Huijing Jin Shuangjun Zhang Haiyan Yang Source Type: research

Gold nanoparticles enhance microRNA 31 detection in colon cancer cells after inhibition with chlorogenic acid
Oncol Lett. 2021 Oct;22(4):742. doi: 10.3892/ol.2021.13003. Epub 2021 Aug 19.ABSTRACTIn the present study, the inhibitory effect of chlorogenic acid (CGA), a phenolic compound with potential antitumor effects, on circulating microRNA 31 (miR-31), was evaluated in RKO colon cancer cells. The capacity of gold nanoparticles (AuNPs) to enhance miR-31 quantification after treatment with CGA was assessed. RKO cells were treated with different concentrations of CGA for 24, 48 and 72 h, after which AuNPs coupled to CD81 were added to the supernatants. Total RNA was extracted, and miR-31 was quantified by reverse transcription-quan...
Source: Oncology Letters - September 1, 2021 Category: Cancer & Oncology Authors: Ana C Luque-Badillo Griselda Hernandez-Tapia Dhamar A Ramirez-Castillo Diego Espinoza-Serrano Alan M Cortes-Limon Juan P Cortes-Gallardo Daniel A Jacobo-Vel ázquez Margarita L Martinez-Fierro Clara P Rios-Ibarra Source Type: research

Pioglitazone mediates apoptosis in Caki cells via downregulating c-FLIP < sub > (L) < /sub > expression and reducing Bcl-2 protein stability
In conclusion, these findings indicated that pioglitazone-induced apoptosis is regulated through caspase-mediated degradation of FLIP(L) and reduction of Bcl-2 protein stability, suggesting that pioglitazone is a feasible apoptotic agent that could be used in the treatment of human RC.PMID:34466155 | PMC:PMC8387863 | DOI:10.3892/ol.2021.13004 (Source: Oncology Letters)
Source: Oncology Letters - September 1, 2021 Category: Cancer & Oncology Authors: Ji Hoon Jang Tae-Jin Lee Eon-Gi Sung In-Hwan Song Joo-Young Kim Source Type: research

Repositioning of duloxetine to target pancreatic stellate cells
The objective of the current study was to identify compounds that suppress PSC activation. Gene expression profiles of cancer-derived fibroblasts and normal fibroblasts were used, and the pathway analysis suggested altered pathways that were chosen for validation. It was found that the 'neuroactive ligand-receptor interaction' pathway from the Kyoto Encyclopedia of Genes and Genomes pathway analysis was one of the altered pathways. Several compounds related with this pathway were chosen, and changes in PSC activity were investigated using fluorescence staining of lipid droplets, reverse transcription-quantitative PCR, west...
Source: Oncology Letters - September 1, 2021 Category: Cancer & Oncology Authors: Akiko Sagara Kohei Nakata Sokichi Matsumoto Weiyu Guan Tomohiko Shinkawa Chika Iwamoto Naoki Ikenaga Kenoki Ohuchida Masafumi Nakamura Source Type: research

Analysis of < em > RHOA < /em > mutations and their significance in the proliferation and transcriptome of digestive tract cancer cells
Oncol Lett. 2021 Oct;22(4):735. doi: 10.3892/ol.2021.12996. Epub 2021 Aug 11.ABSTRACTThe ras homolog family member A (RHOA) gene encodes a member of the Rho family of small GTPases and is known to function in reorganization of the actin cytoskeleton, which is associated with regulation of cell shape, attachment and motility. RHOA has been found to be recurrently mutated in gastrointestinal cancer; however, the functional significance of the mutated RHOA protein in digestive tract cancers remains to be uncovered. The aim of the present study was to understand the role of mutant RHOA in the proliferation and transcriptome of...
Source: Oncology Letters - September 1, 2021 Category: Cancer & Oncology Authors: Naoki Ikari Akiko Serizawa Etsuko Tanji Masakazu Yamamoto Toru Furukawa Source Type: research

TAGLN2 promotes the proliferation, invasion, migration and epithelial-mesenchymal transition of colorectal cancer cells by activating STAT3 signaling through ANXA2
Oncol Lett. 2021 Oct;22(4):737. doi: 10.3892/ol.2021.12998. Epub 2021 Aug 16.ABSTRACTColorectal cancer (CRC) is one of the leading causes of cancer-associated mortality worldwide and currently ranks third in the USA in terms of prevalence. Transgelin-2 (TAGLN2) was previously reported to serve as a tumor promoter in various types of cancer. The present study aimed to investigate the role of TAGLN2 in the progression of CRC and to determine the potential underlying mechanism. The expression level of TAGLN2 in CRC cells (HCT116, SNU-C1, LoVo and SW480) were first detected by reverse transcription quantitative PCR and western...
Source: Oncology Letters - September 1, 2021 Category: Cancer & Oncology Authors: Zhicheng Zhao Li Lu Weidong Li Source Type: research

Different properties of mammary carcinogenesis induced by two chemical carcinogens, DMBA and PhIP, in heterozygous BALB/c < em > Trp53 < /em > knockout mice
In conclusion, the present mouse mammary carcinogenesis models, induced by a combination of genetic and exogenous factors, may be utilized (such as the DMBA-induced model with Trp53 gene function deficiency as a model of adenomyoepithelioma, characterized by distinct biphasic cell constituents and Hras mutations), but PhIP-induced models are required to further analyze the genetic/epigenetic mechanisms promoting mammary carcinomas.PMID:34466150 | PMC:PMC8387855 | DOI:10.3892/ol.2021.12999 (Source: Oncology Letters)
Source: Oncology Letters - September 1, 2021 Category: Cancer & Oncology Authors: Yukino Machida Toshio Imai Source Type: research

Upregulation of microfibrillar-associated protein 2 is closely associated with tumor angiogenesis and poor prognosis in hepatocellular carcinoma
Oncol Lett. 2021 Oct;22(4):739. doi: 10.3892/ol.2021.13000. Epub 2021 Aug 16.ABSTRACTAbnormal expression of microfibrillar-associated protein 2 (MFAP2), a key regulator of cellular differentiation, affects the occurrence and progression of tumors. However, the underlying role of MAFP2 in hepatocellular carcinoma (HCC) remains unclear. In the present study, patterns of MFAP2 expression in HCC were analyzed using sequencing data from The Cancer Genome Atlas database. Expression profiles of MFAP2, as well as those of epithelial-mesenchymal transition (EMT)-related proteins, were compared between HCC pathological sections and ...
Source: Oncology Letters - September 1, 2021 Category: Cancer & Oncology Authors: Nu Zhang Feng Shao Weidong Jia Source Type: research

Lanatoside C inhibits human cervical cancer cell proliferation and induces cell apoptosis by a reduction of the JAK2/STAT6/SOCS2 signaling pathway
Oncol Lett. 2021 Oct;22(4):740. doi: 10.3892/ol.2021.13001. Epub 2021 Aug 17.ABSTRACTCervical cancer is one of the leading causes of cancer-associated mortality in gynecological diseases and ranks third among female cancers worldwide. Although early detection and vaccination have reduced incidence rates, cancer recurrence and metastasis lead to high mortality due to the lack of effective medicines. The present study aimed to identify novel drug candidates to treat cervical cancer. In the present study, lanatoside C, an FDA-approved cardiac glycoside used for the treatment of heart failure, was demonstrated to have anti-pro...
Source: Oncology Letters - September 1, 2021 Category: Cancer & Oncology Authors: Yingchun Duan Li Chen Juan Shao Cui Jiang Yingmei Zhao Yanyi Li Huihui Ke Rui Zhang Jianlong Zhu Minghua Yu Source Type: research

Erratum: MicroRNA-100 functions as a tumor suppressor in cervical cancer via downregulating the SATB1 expression and regulating AKT/mTOR signaling pathway and epithelial-to-mesenchymal transition
Oncol Lett. 2021 Oct;22(4):741. doi: 10.3892/ol.2021.13002. Epub 2021 Aug 17.ABSTRACT[This corrects the article DOI: 10.3892/ol.2020.11686.].PMID:34466153 | PMC:PMC8387881 | DOI:10.3892/ol.2021.13002 (Source: Oncology Letters)
Source: Oncology Letters - September 1, 2021 Category: Cancer & Oncology Authors: Cuiping Huang Xiaobo Qin Na Zhao Huijing Jin Shuangjun Zhang Haiyan Yang Source Type: research

Gold nanoparticles enhance microRNA 31 detection in colon cancer cells after inhibition with chlorogenic acid
Oncol Lett. 2021 Oct;22(4):742. doi: 10.3892/ol.2021.13003. Epub 2021 Aug 19.ABSTRACTIn the present study, the inhibitory effect of chlorogenic acid (CGA), a phenolic compound with potential antitumor effects, on circulating microRNA 31 (miR-31), was evaluated in RKO colon cancer cells. The capacity of gold nanoparticles (AuNPs) to enhance miR-31 quantification after treatment with CGA was assessed. RKO cells were treated with different concentrations of CGA for 24, 48 and 72 h, after which AuNPs coupled to CD81 were added to the supernatants. Total RNA was extracted, and miR-31 was quantified by reverse transcription-quan...
Source: Oncology Letters - September 1, 2021 Category: Cancer & Oncology Authors: Ana C Luque-Badillo Griselda Hernandez-Tapia Dhamar A Ramirez-Castillo Diego Espinoza-Serrano Alan M Cortes-Limon Juan P Cortes-Gallardo Daniel A Jacobo-Vel ázquez Margarita L Martinez-Fierro Clara P Rios-Ibarra Source Type: research

Pioglitazone mediates apoptosis in Caki cells via downregulating c-FLIP < sub > (L) < /sub > expression and reducing Bcl-2 protein stability
In conclusion, these findings indicated that pioglitazone-induced apoptosis is regulated through caspase-mediated degradation of FLIP(L) and reduction of Bcl-2 protein stability, suggesting that pioglitazone is a feasible apoptotic agent that could be used in the treatment of human RC.PMID:34466155 | PMC:PMC8387863 | DOI:10.3892/ol.2021.13004 (Source: Oncology Letters)
Source: Oncology Letters - September 1, 2021 Category: Cancer & Oncology Authors: Ji Hoon Jang Tae-Jin Lee Eon-Gi Sung In-Hwan Song Joo-Young Kim Source Type: research

Repositioning of duloxetine to target pancreatic stellate cells
The objective of the current study was to identify compounds that suppress PSC activation. Gene expression profiles of cancer-derived fibroblasts and normal fibroblasts were used, and the pathway analysis suggested altered pathways that were chosen for validation. It was found that the 'neuroactive ligand-receptor interaction' pathway from the Kyoto Encyclopedia of Genes and Genomes pathway analysis was one of the altered pathways. Several compounds related with this pathway were chosen, and changes in PSC activity were investigated using fluorescence staining of lipid droplets, reverse transcription-quantitative PCR, west...
Source: Oncology Letters - September 1, 2021 Category: Cancer & Oncology Authors: Akiko Sagara Kohei Nakata Sokichi Matsumoto Weiyu Guan Tomohiko Shinkawa Chika Iwamoto Naoki Ikenaga Kenoki Ohuchida Masafumi Nakamura Source Type: research

Analysis of < em > RHOA < /em > mutations and their significance in the proliferation and transcriptome of digestive tract cancer cells
Oncol Lett. 2021 Oct;22(4):735. doi: 10.3892/ol.2021.12996. Epub 2021 Aug 11.ABSTRACTThe ras homolog family member A (RHOA) gene encodes a member of the Rho family of small GTPases and is known to function in reorganization of the actin cytoskeleton, which is associated with regulation of cell shape, attachment and motility. RHOA has been found to be recurrently mutated in gastrointestinal cancer; however, the functional significance of the mutated RHOA protein in digestive tract cancers remains to be uncovered. The aim of the present study was to understand the role of mutant RHOA in the proliferation and transcriptome of...
Source: Oncology Letters - September 1, 2021 Category: Cancer & Oncology Authors: Naoki Ikari Akiko Serizawa Etsuko Tanji Masakazu Yamamoto Toru Furukawa Source Type: research

TAGLN2 promotes the proliferation, invasion, migration and epithelial-mesenchymal transition of colorectal cancer cells by activating STAT3 signaling through ANXA2
Oncol Lett. 2021 Oct;22(4):737. doi: 10.3892/ol.2021.12998. Epub 2021 Aug 16.ABSTRACTColorectal cancer (CRC) is one of the leading causes of cancer-associated mortality worldwide and currently ranks third in the USA in terms of prevalence. Transgelin-2 (TAGLN2) was previously reported to serve as a tumor promoter in various types of cancer. The present study aimed to investigate the role of TAGLN2 in the progression of CRC and to determine the potential underlying mechanism. The expression level of TAGLN2 in CRC cells (HCT116, SNU-C1, LoVo and SW480) were first detected by reverse transcription quantitative PCR and western...
Source: Oncology Letters - September 1, 2021 Category: Cancer & Oncology Authors: Zhicheng Zhao Li Lu Weidong Li Source Type: research

Different properties of mammary carcinogenesis induced by two chemical carcinogens, DMBA and PhIP, in heterozygous BALB/c < em > Trp53 < /em > knockout mice
In conclusion, the present mouse mammary carcinogenesis models, induced by a combination of genetic and exogenous factors, may be utilized (such as the DMBA-induced model with Trp53 gene function deficiency as a model of adenomyoepithelioma, characterized by distinct biphasic cell constituents and Hras mutations), but PhIP-induced models are required to further analyze the genetic/epigenetic mechanisms promoting mammary carcinomas.PMID:34466150 | PMC:PMC8387855 | DOI:10.3892/ol.2021.12999 (Source: Oncology Letters)
Source: Oncology Letters - September 1, 2021 Category: Cancer & Oncology Authors: Yukino Machida Toshio Imai Source Type: research

Upregulation of microfibrillar-associated protein 2 is closely associated with tumor angiogenesis and poor prognosis in hepatocellular carcinoma
Oncol Lett. 2021 Oct;22(4):739. doi: 10.3892/ol.2021.13000. Epub 2021 Aug 16.ABSTRACTAbnormal expression of microfibrillar-associated protein 2 (MFAP2), a key regulator of cellular differentiation, affects the occurrence and progression of tumors. However, the underlying role of MAFP2 in hepatocellular carcinoma (HCC) remains unclear. In the present study, patterns of MFAP2 expression in HCC were analyzed using sequencing data from The Cancer Genome Atlas database. Expression profiles of MFAP2, as well as those of epithelial-mesenchymal transition (EMT)-related proteins, were compared between HCC pathological sections and ...
Source: Oncology Letters - September 1, 2021 Category: Cancer & Oncology Authors: Nu Zhang Feng Shao Weidong Jia Source Type: research

Lanatoside C inhibits human cervical cancer cell proliferation and induces cell apoptosis by a reduction of the JAK2/STAT6/SOCS2 signaling pathway
Oncol Lett. 2021 Oct;22(4):740. doi: 10.3892/ol.2021.13001. Epub 2021 Aug 17.ABSTRACTCervical cancer is one of the leading causes of cancer-associated mortality in gynecological diseases and ranks third among female cancers worldwide. Although early detection and vaccination have reduced incidence rates, cancer recurrence and metastasis lead to high mortality due to the lack of effective medicines. The present study aimed to identify novel drug candidates to treat cervical cancer. In the present study, lanatoside C, an FDA-approved cardiac glycoside used for the treatment of heart failure, was demonstrated to have anti-pro...
Source: Oncology Letters - September 1, 2021 Category: Cancer & Oncology Authors: Yingchun Duan Li Chen Juan Shao Cui Jiang Yingmei Zhao Yanyi Li Huihui Ke Rui Zhang Jianlong Zhu Minghua Yu Source Type: research

Erratum: MicroRNA-100 functions as a tumor suppressor in cervical cancer via downregulating the SATB1 expression and regulating AKT/mTOR signaling pathway and epithelial-to-mesenchymal transition
Oncol Lett. 2021 Oct;22(4):741. doi: 10.3892/ol.2021.13002. Epub 2021 Aug 17.ABSTRACT[This corrects the article DOI: 10.3892/ol.2020.11686.].PMID:34466153 | PMC:PMC8387881 | DOI:10.3892/ol.2021.13002 (Source: Oncology Letters)
Source: Oncology Letters - September 1, 2021 Category: Cancer & Oncology Authors: Cuiping Huang Xiaobo Qin Na Zhao Huijing Jin Shuangjun Zhang Haiyan Yang Source Type: research

Gold nanoparticles enhance microRNA 31 detection in colon cancer cells after inhibition with chlorogenic acid
Oncol Lett. 2021 Oct;22(4):742. doi: 10.3892/ol.2021.13003. Epub 2021 Aug 19.ABSTRACTIn the present study, the inhibitory effect of chlorogenic acid (CGA), a phenolic compound with potential antitumor effects, on circulating microRNA 31 (miR-31), was evaluated in RKO colon cancer cells. The capacity of gold nanoparticles (AuNPs) to enhance miR-31 quantification after treatment with CGA was assessed. RKO cells were treated with different concentrations of CGA for 24, 48 and 72 h, after which AuNPs coupled to CD81 were added to the supernatants. Total RNA was extracted, and miR-31 was quantified by reverse transcription-quan...
Source: Oncology Letters - September 1, 2021 Category: Cancer & Oncology Authors: Ana C Luque-Badillo Griselda Hernandez-Tapia Dhamar A Ramirez-Castillo Diego Espinoza-Serrano Alan M Cortes-Limon Juan P Cortes-Gallardo Daniel A Jacobo-Vel ázquez Margarita L Martinez-Fierro Clara P Rios-Ibarra Source Type: research

Pioglitazone mediates apoptosis in Caki cells via downregulating c-FLIP < sub > (L) < /sub > expression and reducing Bcl-2 protein stability
In conclusion, these findings indicated that pioglitazone-induced apoptosis is regulated through caspase-mediated degradation of FLIP(L) and reduction of Bcl-2 protein stability, suggesting that pioglitazone is a feasible apoptotic agent that could be used in the treatment of human RC.PMID:34466155 | PMC:PMC8387863 | DOI:10.3892/ol.2021.13004 (Source: Oncology Letters)
Source: Oncology Letters - September 1, 2021 Category: Cancer & Oncology Authors: Ji Hoon Jang Tae-Jin Lee Eon-Gi Sung In-Hwan Song Joo-Young Kim Source Type: research

Repositioning of duloxetine to target pancreatic stellate cells
The objective of the current study was to identify compounds that suppress PSC activation. Gene expression profiles of cancer-derived fibroblasts and normal fibroblasts were used, and the pathway analysis suggested altered pathways that were chosen for validation. It was found that the 'neuroactive ligand-receptor interaction' pathway from the Kyoto Encyclopedia of Genes and Genomes pathway analysis was one of the altered pathways. Several compounds related with this pathway were chosen, and changes in PSC activity were investigated using fluorescence staining of lipid droplets, reverse transcription-quantitative PCR, west...
Source: Oncology Letters - September 1, 2021 Category: Cancer & Oncology Authors: Akiko Sagara Kohei Nakata Sokichi Matsumoto Weiyu Guan Tomohiko Shinkawa Chika Iwamoto Naoki Ikenaga Kenoki Ohuchida Masafumi Nakamura Source Type: research

Analysis of < em > RHOA < /em > mutations and their significance in the proliferation and transcriptome of digestive tract cancer cells
Oncol Lett. 2021 Oct;22(4):735. doi: 10.3892/ol.2021.12996. Epub 2021 Aug 11.ABSTRACTThe ras homolog family member A (RHOA) gene encodes a member of the Rho family of small GTPases and is known to function in reorganization of the actin cytoskeleton, which is associated with regulation of cell shape, attachment and motility. RHOA has been found to be recurrently mutated in gastrointestinal cancer; however, the functional significance of the mutated RHOA protein in digestive tract cancers remains to be uncovered. The aim of the present study was to understand the role of mutant RHOA in the proliferation and transcriptome of...
Source: Oncology Letters - September 1, 2021 Category: Cancer & Oncology Authors: Naoki Ikari Akiko Serizawa Etsuko Tanji Masakazu Yamamoto Toru Furukawa Source Type: research

TAGLN2 promotes the proliferation, invasion, migration and epithelial-mesenchymal transition of colorectal cancer cells by activating STAT3 signaling through ANXA2
Oncol Lett. 2021 Oct;22(4):737. doi: 10.3892/ol.2021.12998. Epub 2021 Aug 16.ABSTRACTColorectal cancer (CRC) is one of the leading causes of cancer-associated mortality worldwide and currently ranks third in the USA in terms of prevalence. Transgelin-2 (TAGLN2) was previously reported to serve as a tumor promoter in various types of cancer. The present study aimed to investigate the role of TAGLN2 in the progression of CRC and to determine the potential underlying mechanism. The expression level of TAGLN2 in CRC cells (HCT116, SNU-C1, LoVo and SW480) were first detected by reverse transcription quantitative PCR and western...
Source: Oncology Letters - September 1, 2021 Category: Cancer & Oncology Authors: Zhicheng Zhao Li Lu Weidong Li Source Type: research

Different properties of mammary carcinogenesis induced by two chemical carcinogens, DMBA and PhIP, in heterozygous BALB/c < em > Trp53 < /em > knockout mice
In conclusion, the present mouse mammary carcinogenesis models, induced by a combination of genetic and exogenous factors, may be utilized (such as the DMBA-induced model with Trp53 gene function deficiency as a model of adenomyoepithelioma, characterized by distinct biphasic cell constituents and Hras mutations), but PhIP-induced models are required to further analyze the genetic/epigenetic mechanisms promoting mammary carcinomas.PMID:34466150 | PMC:PMC8387855 | DOI:10.3892/ol.2021.12999 (Source: Oncology Letters)
Source: Oncology Letters - September 1, 2021 Category: Cancer & Oncology Authors: Yukino Machida Toshio Imai Source Type: research

Upregulation of microfibrillar-associated protein 2 is closely associated with tumor angiogenesis and poor prognosis in hepatocellular carcinoma
Oncol Lett. 2021 Oct;22(4):739. doi: 10.3892/ol.2021.13000. Epub 2021 Aug 16.ABSTRACTAbnormal expression of microfibrillar-associated protein 2 (MFAP2), a key regulator of cellular differentiation, affects the occurrence and progression of tumors. However, the underlying role of MAFP2 in hepatocellular carcinoma (HCC) remains unclear. In the present study, patterns of MFAP2 expression in HCC were analyzed using sequencing data from The Cancer Genome Atlas database. Expression profiles of MFAP2, as well as those of epithelial-mesenchymal transition (EMT)-related proteins, were compared between HCC pathological sections and ...
Source: Oncology Letters - September 1, 2021 Category: Cancer & Oncology Authors: Nu Zhang Feng Shao Weidong Jia Source Type: research

Lanatoside C inhibits human cervical cancer cell proliferation and induces cell apoptosis by a reduction of the JAK2/STAT6/SOCS2 signaling pathway
Oncol Lett. 2021 Oct;22(4):740. doi: 10.3892/ol.2021.13001. Epub 2021 Aug 17.ABSTRACTCervical cancer is one of the leading causes of cancer-associated mortality in gynecological diseases and ranks third among female cancers worldwide. Although early detection and vaccination have reduced incidence rates, cancer recurrence and metastasis lead to high mortality due to the lack of effective medicines. The present study aimed to identify novel drug candidates to treat cervical cancer. In the present study, lanatoside C, an FDA-approved cardiac glycoside used for the treatment of heart failure, was demonstrated to have anti-pro...
Source: Oncology Letters - September 1, 2021 Category: Cancer & Oncology Authors: Yingchun Duan Li Chen Juan Shao Cui Jiang Yingmei Zhao Yanyi Li Huihui Ke Rui Zhang Jianlong Zhu Minghua Yu Source Type: research

Erratum: MicroRNA-100 functions as a tumor suppressor in cervical cancer via downregulating the SATB1 expression and regulating AKT/mTOR signaling pathway and epithelial-to-mesenchymal transition
Oncol Lett. 2021 Oct;22(4):741. doi: 10.3892/ol.2021.13002. Epub 2021 Aug 17.ABSTRACT[This corrects the article DOI: 10.3892/ol.2020.11686.].PMID:34466153 | PMC:PMC8387881 | DOI:10.3892/ol.2021.13002 (Source: Oncology Letters)
Source: Oncology Letters - September 1, 2021 Category: Cancer & Oncology Authors: Cuiping Huang Xiaobo Qin Na Zhao Huijing Jin Shuangjun Zhang Haiyan Yang Source Type: research

Gold nanoparticles enhance microRNA 31 detection in colon cancer cells after inhibition with chlorogenic acid
Oncol Lett. 2021 Oct;22(4):742. doi: 10.3892/ol.2021.13003. Epub 2021 Aug 19.ABSTRACTIn the present study, the inhibitory effect of chlorogenic acid (CGA), a phenolic compound with potential antitumor effects, on circulating microRNA 31 (miR-31), was evaluated in RKO colon cancer cells. The capacity of gold nanoparticles (AuNPs) to enhance miR-31 quantification after treatment with CGA was assessed. RKO cells were treated with different concentrations of CGA for 24, 48 and 72 h, after which AuNPs coupled to CD81 were added to the supernatants. Total RNA was extracted, and miR-31 was quantified by reverse transcription-quan...
Source: Oncology Letters - September 1, 2021 Category: Cancer & Oncology Authors: Ana C Luque-Badillo Griselda Hernandez-Tapia Dhamar A Ramirez-Castillo Diego Espinoza-Serrano Alan M Cortes-Limon Juan P Cortes-Gallardo Daniel A Jacobo-Vel ázquez Margarita L Martinez-Fierro Clara P Rios-Ibarra Source Type: research

Pioglitazone mediates apoptosis in Caki cells via downregulating c-FLIP < sub > (L) < /sub > expression and reducing Bcl-2 protein stability
In conclusion, these findings indicated that pioglitazone-induced apoptosis is regulated through caspase-mediated degradation of FLIP(L) and reduction of Bcl-2 protein stability, suggesting that pioglitazone is a feasible apoptotic agent that could be used in the treatment of human RC.PMID:34466155 | PMC:PMC8387863 | DOI:10.3892/ol.2021.13004 (Source: Oncology Letters)
Source: Oncology Letters - September 1, 2021 Category: Cancer & Oncology Authors: Ji Hoon Jang Tae-Jin Lee Eon-Gi Sung In-Hwan Song Joo-Young Kim Source Type: research

Repositioning of duloxetine to target pancreatic stellate cells
The objective of the current study was to identify compounds that suppress PSC activation. Gene expression profiles of cancer-derived fibroblasts and normal fibroblasts were used, and the pathway analysis suggested altered pathways that were chosen for validation. It was found that the 'neuroactive ligand-receptor interaction' pathway from the Kyoto Encyclopedia of Genes and Genomes pathway analysis was one of the altered pathways. Several compounds related with this pathway were chosen, and changes in PSC activity were investigated using fluorescence staining of lipid droplets, reverse transcription-quantitative PCR, west...
Source: Oncology Letters - September 1, 2021 Category: Cancer & Oncology Authors: Akiko Sagara Kohei Nakata Sokichi Matsumoto Weiyu Guan Tomohiko Shinkawa Chika Iwamoto Naoki Ikenaga Kenoki Ohuchida Masafumi Nakamura Source Type: research

Analysis of < em > RHOA < /em > mutations and their significance in the proliferation and transcriptome of digestive tract cancer cells
Oncol Lett. 2021 Oct;22(4):735. doi: 10.3892/ol.2021.12996. Epub 2021 Aug 11.ABSTRACTThe ras homolog family member A (RHOA) gene encodes a member of the Rho family of small GTPases and is known to function in reorganization of the actin cytoskeleton, which is associated with regulation of cell shape, attachment and motility. RHOA has been found to be recurrently mutated in gastrointestinal cancer; however, the functional significance of the mutated RHOA protein in digestive tract cancers remains to be uncovered. The aim of the present study was to understand the role of mutant RHOA in the proliferation and transcriptome of...
Source: Oncology Letters - September 1, 2021 Category: Cancer & Oncology Authors: Naoki Ikari Akiko Serizawa Etsuko Tanji Masakazu Yamamoto Toru Furukawa Source Type: research

TAGLN2 promotes the proliferation, invasion, migration and epithelial-mesenchymal transition of colorectal cancer cells by activating STAT3 signaling through ANXA2
Oncol Lett. 2021 Oct;22(4):737. doi: 10.3892/ol.2021.12998. Epub 2021 Aug 16.ABSTRACTColorectal cancer (CRC) is one of the leading causes of cancer-associated mortality worldwide and currently ranks third in the USA in terms of prevalence. Transgelin-2 (TAGLN2) was previously reported to serve as a tumor promoter in various types of cancer. The present study aimed to investigate the role of TAGLN2 in the progression of CRC and to determine the potential underlying mechanism. The expression level of TAGLN2 in CRC cells (HCT116, SNU-C1, LoVo and SW480) were first detected by reverse transcription quantitative PCR and western...
Source: Oncology Letters - September 1, 2021 Category: Cancer & Oncology Authors: Zhicheng Zhao Li Lu Weidong Li Source Type: research

Different properties of mammary carcinogenesis induced by two chemical carcinogens, DMBA and PhIP, in heterozygous BALB/c < em > Trp53 < /em > knockout mice
In conclusion, the present mouse mammary carcinogenesis models, induced by a combination of genetic and exogenous factors, may be utilized (such as the DMBA-induced model with Trp53 gene function deficiency as a model of adenomyoepithelioma, characterized by distinct biphasic cell constituents and Hras mutations), but PhIP-induced models are required to further analyze the genetic/epigenetic mechanisms promoting mammary carcinomas.PMID:34466150 | PMC:PMC8387855 | DOI:10.3892/ol.2021.12999 (Source: Oncology Letters)
Source: Oncology Letters - September 1, 2021 Category: Cancer & Oncology Authors: Yukino Machida Toshio Imai Source Type: research

Upregulation of microfibrillar-associated protein 2 is closely associated with tumor angiogenesis and poor prognosis in hepatocellular carcinoma
Oncol Lett. 2021 Oct;22(4):739. doi: 10.3892/ol.2021.13000. Epub 2021 Aug 16.ABSTRACTAbnormal expression of microfibrillar-associated protein 2 (MFAP2), a key regulator of cellular differentiation, affects the occurrence and progression of tumors. However, the underlying role of MAFP2 in hepatocellular carcinoma (HCC) remains unclear. In the present study, patterns of MFAP2 expression in HCC were analyzed using sequencing data from The Cancer Genome Atlas database. Expression profiles of MFAP2, as well as those of epithelial-mesenchymal transition (EMT)-related proteins, were compared between HCC pathological sections and ...
Source: Oncology Letters - September 1, 2021 Category: Cancer & Oncology Authors: Nu Zhang Feng Shao Weidong Jia Source Type: research

Lanatoside C inhibits human cervical cancer cell proliferation and induces cell apoptosis by a reduction of the JAK2/STAT6/SOCS2 signaling pathway
Oncol Lett. 2021 Oct;22(4):740. doi: 10.3892/ol.2021.13001. Epub 2021 Aug 17.ABSTRACTCervical cancer is one of the leading causes of cancer-associated mortality in gynecological diseases and ranks third among female cancers worldwide. Although early detection and vaccination have reduced incidence rates, cancer recurrence and metastasis lead to high mortality due to the lack of effective medicines. The present study aimed to identify novel drug candidates to treat cervical cancer. In the present study, lanatoside C, an FDA-approved cardiac glycoside used for the treatment of heart failure, was demonstrated to have anti-pro...
Source: Oncology Letters - September 1, 2021 Category: Cancer & Oncology Authors: Yingchun Duan Li Chen Juan Shao Cui Jiang Yingmei Zhao Yanyi Li Huihui Ke Rui Zhang Jianlong Zhu Minghua Yu Source Type: research