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Activation of Janus kinase 1 confers poor prognosis in patients with non-small cell lung cancer.
Authors: Liu D, Huang Y, Zhang L, Liang DN, Li L Abstract The activation of Janus kinase 1 (JAK1) has been reported to occur in non-small cell lung cancer (NSCLC), activating the JAK/signal transducers and activators of transcription cascade. However, the association between JAK1 activation and the prognostic value in NSCLC remains unclear. The present study initially investigated the association between expression of the activated form of JAK1 (p-JAK1) and prognosis in patients with NSCLC. A cohort of 142 resected primary NSCLC tissue samples, including 74 adenocarcinoma (ADCC) and 68 squamous cell carcinoma sampl...
Source: Oncology Letters - October 12, 2017 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Investigating the microRNA-mRNA regulatory network in acute myeloid leukemia.
Authors: Zhang H, Zhang C, Feng R, Zhang H, Gao M, Ye L Abstract Acute myeloid leukemia (AML) is a common myelogenous malignancy in adults that is often characterized by disease relapse. The pathophysiological mechanism of AML has not yet been elucidated. The present study aimed to identify the crucial microRNAs (miRNAs/miRs) and target genes in AML, and to uncover the potential oncogenic mechanism of AML. miRNA and mRNA expression-profiling microarray datasets were downloaded from the Gene Expression Omnibus database. Differential expression analysis was performed and a regulatory network between miRNAs and target...
Source: Oncology Letters - October 12, 2017 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Mechanisms of inhibiting human leukemia cell lines by serum of rats treated with compound banmao capsule.
In conclusion, CBC-containing serum exerted an inhibitory effect on the growth of K562 and HL60 cells by decreasing cellular proliferation, promoting apoptosis and cell cycle arrest, and decreasing the expression of VEGF-A, and chemotactic and inflammatory genes. PMID: 28989536 [PubMed] (Source: Oncology Letters)
Source: Oncology Letters - October 12, 2017 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Effect of inhibiting Beclin-1 expression on autophagy, proliferation and apoptosis in colorectal cancer.
Authors: Liu L, Zhao WM, Yang XH, Sun ZQ, Jin HZ, Lei C, Jin B, Wang HJ Abstract The present study aimed to investigate the molecular mechanisms and effect of Beclin-1 on autophagy, proliferation and apoptosis in the colorectal cancer (CRC) HCT116 and SW620 cells. Beclin-1 was silenced by RNA interference (RNAi) in HTC116 and SW620 cells. Reverse transcription-polymerase chain reaction and western blot were used to measure the expression of Beclin-1. The percentage of apoptotic cells was analyzed by cell counting kit-8 (CCK-8) and flow cytometry (FCM). Cell cycle and cell proliferation were analyzed by FCM and the ...
Source: Oncology Letters - October 12, 2017 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Erratum: Efficacy and tolerability of lacosamide for secondary epileptic seizures in patients with brain tumor: A multicenter, observational retrospective study.
Authors: Sepúlveda-Sánchez JM, Conde-Moreno A, Barón M, Pardo J, Reynés G, Belenguer A Abstract [This corrects the article DOI: 10.3892/ol.2017.5988.]. PMID: 28989538 [PubMed - in process] (Source: Oncology Letters)
Source: Oncology Letters - October 12, 2017 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Role of wild-type p53-induced phosphatase 1 in cancer.
Authors: Wang ZP, Tian Y, Lin J Abstract Wild-type p53-induced phosphatase (Wip1) is a member of the protein phosphatase type 2C family and is an established oncogene due to its dephosphorylation of several tumor suppressors and negative control of the DNA damage response system. It has been reported to dephosphorylate p53, ataxia telangiectasia mutated, checkpoint kinase 1 and p38 mitogen activated protein kinases, forming negative feedback loops to inhibit apoptosis and cell cycle arrest. Wip1 serves a major role in tumorigenesis, progression, invasion, distant metastasis and apoptosis in various types of human c...
Source: Oncology Letters - September 30, 2017 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Effect of etoposide-induced alteration of the Mdm2-Rb signaling pathway on cellular senescence in A549 lung adenocarcinoma cells.
Authors: Dai W, Jiang Y, Chen K, Qiu J, Sun J, Zhang W, Zhou X, Huang N, Li Y, Li W Abstract The present study aimed to investigate the effect of various concentrations of etoposide (VP-16) on the E3 ubiquitin-protein ligase Mdm2 (Mdm2)-retinoblastoma (Rb) signaling pathway in the cellular senescence of A549 lung adenocarcinoma cells. A549 cells were randomly divided into the following four groups: Control group (no treatment), group 1 (1 µmol/l VP-16), group 2 (5 µmol/l VP-16) and group 3 (25 µmol/l VP-16). Each group was cultured for 48 h after treatment prior to observation of the alterations t...
Source: Oncology Letters - September 30, 2017 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Inhibition of Hes1 enhances lapatinib sensitivity in gastric cancer sphere-forming cells.
Authors: Li L, Li Y, Wang L, Wu Z, Ma H, Shao J, Li D, Yu H, Nian W, Wang D Abstract It has been considered that the neurogenic locus notch homolog protein (Notch) signaling pathway serves an essential role in cellular differentiation, proliferation and apoptosis. However, the function of the Notch signaling pathway in gastric cancer stem cells (GCSCs) and epidermal growth factor receptor-tyrosine kinase inhibitor (EGFR-TKI) sensitivity remains unclear. The present study aimed to delineate the role of the Notch1 signaling pathway in GCSCs and lapatinib sensitivity. Sphere-forming cells were separated from human gas...
Source: Oncology Letters - September 30, 2017 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Relationship of the expression levels of XIAP and p53 genes in hepatocellular carcinoma and the prognosis of patients.
In this study, we measured mRNA and protein expression levels of X-linked inhibitor of apoptosis protein (XIAP) and p53 in hepatocellular carcinoma (HCC) and analyzed their relationships to clinicopathological parameters and the prognosis of the patients. Samples were obtained from tumors and tumor-adjacent normal tissues from 70 patients with HCC who were hospitalized in Weifang People's Hospital from January 2009 to December 2011. Quantitative polymerase chain reaction (qPCR) and immunohistochemistry (IHC) were used to detect the mRNA and protein expression levels, respectively. The clinical data of patients who were fol...
Source: Oncology Letters - September 30, 2017 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Long non-coding RNA MEG3 inhibits the proliferation and metastasis of oral squamous cell carcinoma by regulating the WNT/ β-catenin signaling pathway.
This study aimed to investigate how long non-coding RNA (lncRNA) maternally expressed gene 3 (MEG3) inhibits the growth and metastasis of oral squamous cell carcinoma (OSCC) by regulating WNT/β-catenin signaling pathway in order to explore the antitumor effect of MEG3 and to provide a potential molecular target for the treatment of OSCC. The RT-qPCR technique was used to quantitatively analyze the expression of MEG3 in cancer and adjacent tissues collected from the patients after surgery. Using the Lipofectamine method, the MEG3 overexpression vector and the siRNA interference vector were constructed and transfected i...
Source: Oncology Letters - September 30, 2017 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Intensity-modulated radiotherapy combined with iodine-125 seed implantation in non-central recurrence of cervical cancer: A case report and literature review.
Authors: Feng H, Hu Y, Jin P, Meng X, Chen Y, Zhang H Abstract Recurrent cervical cancer is a clinically complex disease that is difficult to treat. There are numerous treatment options, but the results achieved by each are poor. External-beam radiation therapy of the pelvic lymph drainage area, in combination with intracavitary afterloading or the interstitial implantation of a radiation source (i.e., brachytherapy), are the current standard radiotherapy regimens used in high-risk clinical targets. However, there are few reports concerning the use of iodine-125 ((125)I) seed implantation brachytherapy in recurrent...
Source: Oncology Letters - September 30, 2017 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Mode of action of pertuzumab in combination with trastuzumab plus docetaxel therapy in a HER2-positive breast cancer xenograft model.
In this study, we investigated the mechanism of action of the triple-drug combination therapy in vivo. For this purpose, we established a mouse xenograft model using KPL-4, a HER2-positive human breast cancer cell line, in which the triple-drug combination treatment dramatically induced tumor regression compared with double-drug combinations (trastuzumab plus docetaxel, pertuzumab plus docetaxel, or pertuzumab plus trastuzumab). Four days after the triple-drug treatment was started, strong reduction in the phosphorylation of HER2, epidermal growth factor receptor (EGFR), HER3, extracellular signal-regulated kinase (ERK), a...
Source: Oncology Letters - September 30, 2017 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Elacridar, a third-generation ABCB1 inhibitor, overcomes resistance to docetaxel in non-small cell lung cancer.
Authors: Chen H, Shien K, Suzawa K, Tsukuda K, Tomida S, Sato H, Torigoe H, Watanabe M, Namba K, Yamamoto H, Soh J, Asano H, Miyoshi S, Toyooka S Abstract Docetaxel is a third-generation chemotherapeutic drug that is widely used in the treatment of patients with non-small cell lung cancer (NSCLC). However, the majority of patients with NSCLC eventually acquire resistance to the treatment. In the present study, the mechanism of acquired resistance to docetaxel treatment in lung cancer cells was investigated. The three NSCLC cell lines, H1299 with wild-type epidermal growth factor receptor (EGFR), EGFR-mutant HCC4006...
Source: Oncology Letters - September 30, 2017 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Assessment of bone metabolism and biomechanical properties of the femur, following treatment with anastrozole and letrozole in an experimental model of menopause.
Authors: Boutas I, Pergialiotis V, Salakos N, Korou LM, Mitousoudis A, Kalampokas E, Deligeoroglou E, Gregoriou O, Perrea DN, Creatsas G, Kourkoulis S Abstract The aim of the present study was to investigate the impact of anastrozole and letrozole supplementation following surgically induced menopause on bone metabolism and biomechanical properties. A total of 45 Wistar rats underwent ovariectomy and were then randomly allocated to receive no treatment, anastrozole or letrozole. At 2 and 4 months following the initiation of the present study, the serum levels of osteoprotegerin (OPG) and receptor activator of nucle...
Source: Oncology Letters - September 26, 2017 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

A large Chinese pedigree of multiple endocrine neoplasia type 2A with a novel C634Y/D707E germline mutation in RET exon 11.
Authors: Lu F, Chen X, Bai Y, Feng Y, Wu J Abstract The present study identified the clinical features of the largest multiple endocrine neoplasia type 2 (MEN2) A pedigree from China, with a novel double missense rearranged during transfection (RET) mutation (C634Y/D707E). To the best of our knowledge, the D707E mutation has not been identified to date. In the present study, a total of 101 family members who originated from a large pedigree (134 members in total) underwent RET mutation screening by next-generation sequencing and polymerase chain reaction (PCR) amplification, followed by direct bidirectional DNA seq...
Source: Oncology Letters - September 26, 2017 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Role of liver ICAM-1 in metastasis.
Authors: Benedicto A, Romayor I, Arteta B Abstract Intercellular adhesion molecule (ICAM)-1, is a transmembrane glycoprotein of the immunoglobulin (Ig)-like superfamily, consisting of five extracellular Ig-like domains, a transmembrane domain and a short cytoplasmic tail. ICAM-1 is expressed in various cell types, including endothelial cells and leukocytes, and is involved in several physiological processes. Furthermore, it has additionally been reported to be expressed in various cancer cells, including melanoma, colorectal cancer and lymphoma. The majority of studies to date have focused on the expression of the ...
Source: Oncology Letters - September 26, 2017 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Metastasis-associated in colon cancer 1: A promising biomarker for the metastasis and prognosis of colorectal cancer.
Authors: Li H, Chen YX, Wen JG, Zhou HH Abstract Colorectal cancer (CRC) is the fourth most frequent type of malignancy in the world. Metastasis accounts for>90% mortalities in patients with CRC. The metastasis-associated in colon cancer 1 (MACC1) gene has been identified as a novel biomarker for the prediction of metastasis and disease prognosis, particularly for patients with early-stage disease. Previous clinical studies demonstrated that MACC1 expression and polymorphisms in CRC tissues were indicators of metastasis, and that circulating transcripts in plasma were also significantly associated with the survi...
Source: Oncology Letters - September 26, 2017 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Surgical management of pelvic Ewing's sarcoma in children and adolescents.
Authors: Fan H, Guo Z, Fu J, Li X, Li J, Wang Z Abstract The present study describes a novel surgical strategy used to treat immature pelvic Ewing's sarcoma (ES), one made possible owing to the intrinsic structure of the skeletally immature pelvis. A total of 12 children and adolescents with open triradiate cartilage received limb-salvage surgeries following a diagnosis of pelvic ES. In total, 3 patients with iliac lesions (2 lesions with extension into the sacrum) received surgical tumor excisions and allograft reconstructions. Another 8 patients with periacetabular lesions received trans-acetabular osteotomies an...
Source: Oncology Letters - September 26, 2017 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Influence of chronic inflammation on Bcl-2 and PCNA expression in prostate needle biopsy specimens.
Authors: Glover M, Soni S, Ren Q, Maclennan GT, Fu P, Gupta S Abstract The association between inflammation and cancer has been established in certain forms of human malignancies; however, its role in prostate cancer remains unclear. The present study investigates a possible association between chronic inflammation and the development of epithelial neoplasia in the prostate. Needle biopsy specimens were obtained from patients with serum prostate-specific antigen levels>4 ng/ml, evaluated for morphological findings, and immunostained for Bcl-2 and proliferating cell nuclear antigen (PCNA). Bcl-2 is a survival pro...
Source: Oncology Letters - September 26, 2017 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

miR-590 promotes the proliferation of HUMSCs and induces ECM synthesis by targeting Smad7.
Authors: Liu T, Wu Y, Huang T, Zhang X, Cai Y Abstract MicroRNA (miR)-590 has been established to be a promoter of cell proliferation, migration and invasion, and an inhibitor of apoptosis in numerous cancer cell lines. However, its effects on non-cancer cells remain to be elucidated. miR-590 was transfected into human umbilical cord mesenchymal stem cells (HUMSCs), and the cell proliferation rate was determined using a Cell-Light 5-ethynyl-20-deoxyuridine Apollo 567 kit and the presence of extracellular matrix (ECM) proteins were detected using western blot analysis and immunofluorescence microscopy. Using bioinfo...
Source: Oncology Letters - September 26, 2017 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Khat promotes human breast cancer MDA-MB-231 cell apoptosis via mitochondria and MAPK-associated pathways.
Authors: Lu Y, Li Y, Xiang M, Zhou J, Chen J Abstract Khat (Catha edulis Forsk) is a flowering evergreen plant in Eastern Africa and Southwestern Arabia. Consumption of Khat has been associated with the development of oral cancer, but its mechanism of action on the molecular level remains unclear. The present study demonstrated the cytotoxic effect of khat extracts on the human breast cancer cell line MDA-MB-231. Trypan blue exclusion assays, flow cytometry, fluorescent and electron microscopy, as well as western blotting were used to analyze the effects of Khat on the cell viability of breast cancer cells, express...
Source: Oncology Letters - September 26, 2017 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

High expression of GP73 in primary hepatocellular carcinoma and its function in the assessment of transcatheter arterial chemoembolization.
Authors: Ai N, Liu W, Li ZG, Ji H, Li B, Yang G Abstract Golgi glycoprotein 73 (GP73) is a type II Golgi transmembrane protein and a potential novel marker for the diagnosis of primary hepatocellular carcinoma (PHC). However, its reliability as a serum marker for the diagnosis of PHC following transcatheter arterial chemoembolization (TACE) remains unknown. The aim of the present study was to evaluate the diagnostic value of serum GP73 levels in patients with PHC, to determine its diagnostic accuracy in patients with PHC following TACE. Reverse transcription-quantitative polymerase chain reaction analysis was used ...
Source: Oncology Letters - September 26, 2017 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Identification of candidate biomarkers for epithelial ovarian cancer metastasis using microarray data.
Authors: Li S, Li H, Xu Y, Lv X Abstract Epithelial ovarian cancer (EOC) is a common cancer in women worldwide. The present study assessed effective biomarkers for the prognosis of EOC metastasis. The GSE30587 dataset, containing 9 EOC primary tumor samples and 9 matched omental metastasis samples, was analyzed. Following normalization, the differentially expressed genes (DEGs) between these samples were identified using the limma package for R. Subsequently, pathway enrichment analysis was performed using ClueGO, and a protein-protein interaction (PPI) network was constructed using the Search Tool for the Retrieva...
Source: Oncology Letters - September 26, 2017 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Screening the key genes of hepatocellular adenoma via microarray analysis of DNA expression and methylation profiles.
Authors: Liu D, Liu P, Cao L, Zhang Q, Chen Y Abstract The aim of the present study was to identify the biomarkers involved in the development of hepatocellular adenoma (HCA) through integrated analysis of gene expression and methylation microarray. The microarray dataset GSE7473, containing HNF1α-mutated HCA and their corresponding non-tumor livers, 5 HNF1α-mutated HCA and 4 non-related non-tumor livers, was downloaded from the Gene Expression Omnibus (GEO) database. The DNA methylation profile GSE43091, consisting of 50 HCA and 4 normal liver tissues, was also downloaded from the GEO database. Differe...
Source: Oncology Letters - September 26, 2017 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Hepatectomy for spontaneous rupture of hepatocellular carcinoma without portal triad clamping.
Authors: Wang ZM, Lu XS, Qiu F Abstract Hepatectomy without portal triad clamping may decrease the incidence of liver injury; however, the effects of hepatectomy without portal triad clamping in the treatment of spontaneous rupture of hepatocellular carcinoma (SRHCC) remain unclear. The aims of the present study were to evaluate the therapeutic value of hepatectomy without portal triad clamping in the treatment of patients with SRHCC. The present study retrospectively reviewed patients with SRHCC who received hepatectomy without portal triad clamping (non-clamping group) and the therapeutic efficacy was compared wi...
Source: Oncology Letters - September 26, 2017 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Total en bloc spondylectomy of the eleventh thoracic vertebra following denosumab therapy for the treatment of a giant cell tumor.
Authors: Inoue G, Imura T, Miyagi M, Saito W, Tazawa R, Nakazawa T, Takaso M Abstract Although denosumab has been reported to induce effective clinical results with respect to tumor shrinkage in a short-term follow-up clinical study, total spondylectomy is recognized as the treatment of choice for eradicating giant cell tumors (GCTs) of the spine. The present study reports the case involving a GCT in the 11th thoracic vertebra complicated by idiopathic scoliosis and treated using total en bloc spondylectomy (TES) with preoperative denosumab therapy. A 35-year-old woman received preoperative denosumab therapy for 8 ...
Source: Oncology Letters - September 26, 2017 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Expression of chicken ovalbumin upstream promoter-transcription factor II and liver X receptor as prognostic indicators for human colorectal cancer.
Authors: Yun SH, Park MG, Kim YM, Roh MS, Park JI Abstract Cholesterol increases the risk of colorectal cancer. Liver X receptor (LXR), retinoid X receptor (RXR)α and sterol regulatory element binding protein (SREBP)-1c are transcriptional regulators of lipid metabolism. Chicken ovalbumin upstream promoter-transcription factor II (COUP-TFII) serves an essential role in angiogenesis and development, but its role in cancer is controversial. The expression of COUP-TFII, LXR, RXRα and SREBP-1c in colorectal cancer, as well as their association with clinicopathologic features, was assessed, and their utility...
Source: Oncology Letters - September 26, 2017 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Downregulation of LRIG2 expression inhibits angiogenesis of glioma via EGFR/VEGF-A pathway.
Authors: Yang HK, Chen H, Mao F, Xiao QG, Xie RF, Lei T Abstract Active angiogenesis is the basic pathological feature of glioma. Tumor angiogenesis is involved in vascular endothelial cell migration to the tumor tissue and in the formation of tube-like structures. The present study aimed to investigate the role of leucine-rich repeats and immunoglobulin-like domains 2 (LRIG2) in glioma angiogenesis. Glioma (n=50) and normal brain (n=20) tissue samples were collected from patients to detect the expression of LRIG2, epidermal growth factor receptor (EGFR), vascular endothelial growth factor A (VEGF-A), and cluster o...
Source: Oncology Letters - September 26, 2017 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Wound fluids affect miR-21, miR-155 and miR-221 expression in breast cancer cell lines, and this effect is partially abrogated by intraoperative radiation therapy treatment.
Authors: Zaleska K, Przybyła A, Kulcenty K, Wichtowski M, Mackiewicz A, Suchorska W, Murawa D Abstract Breast cancer is the most common malignant disease occurring in women. Conservative breast cancer surgery followed by radiation therapy is currently the standard treatment for this type of cancer. The majority of metastases occur within the scar, which initiated a series of studies. As a result, clinical trials aimed to assess whether localized radiotherapy, as intraoperative radiotherapy (IORT), may more effective in inhibiting the formation of local recurrence compared with the standard postoperative whole brea...
Source: Oncology Letters - September 26, 2017 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Microplate magnetic chemiluminescence immunoassay for detecting urinary survivin in bladder cancer.
Authors: Chang Y, Xu J, Zhang Q Abstract Survivin is a tumor marker for bladder cancer; however the role of urinary survivin levels has not been fully elucidated due to the limitations of current detection methods. Based on two survivin-specific monoclonal antibodies (McAbs) already confirmed through enzyme linked immunosorbent assays, the present study aimed to establish a microplate magnetic chemiluminescence immunoassay (CLIA) for the detection of urinary survivin levels and evaluate its application for the diagnosis of patients with bladder cancer. Horseradish peroxidase and biotin conjugates were used to label...
Source: Oncology Letters - September 26, 2017 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Effect of Hospital to Home nutrition management model on postoperative clinical outcomes of patients with laryngeal carcinoma.
Authors: Song G, Liu H Abstract The purpose of this investigation was to determine the effectiveness of a Hospital to Home (H2H) nutrition management model on the postoperative clinical outcomes of patients with laryngeal carcinoma. Eighty-six patients with laryngeal carcinoma were randomly divided into an observation (H2H nutrition management) or control group (routine nutrition management). The ratios of urine lactulose to mannitol (L/M) in serum orexin-A levels of patients in both groups on the 1st postoperative day were significantly higher than before surgery; these levels decreased from the 7th and 14th posto...
Source: Oncology Letters - September 26, 2017 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Relationship between the expression of CD133, HIF-1 α, VEGF and the proliferation and apoptosis in hypoxic human prostate cancer cells.
This study measured the levels of expression of CD133, hypoxia-inducible factor (HIF)-1α and vascular endothelial growth factor (VEGF) in human prostate cancer cells grown under hypoxic and non-hypoxic conditions to compare the values to resulting amounts of proliferation and apoptosis in the cells. Human prostate cancer cell line LNCaP cells were routinely thawed, cultured and passaged. Actively growing cells were divided into batches. Cells in the control group were grown under 5% CO2 + 20% O2, and those in the hypoxia group were grown under 5% CO2 + 1% O2. The experiments were performed after 12, 24 and 72 h under...
Source: Oncology Letters - September 26, 2017 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Metastatic gastric cancer from breast carcinoma: A report of 78 cases.
In conclusion, physicians must be vigilant when patients with breast cancer history present with gastrointestinal symptoms, despite gastric metastasis from breast cancer being rare. An appropriate systemic therapeutic strategy that includes hormonal therapy may be beneficial for this group of patients. PMID: 28943914 [PubMed] (Source: Oncology Letters)
Source: Oncology Letters - September 26, 2017 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Zoledronic acid inhibits infiltration of tumor-associated macrophages and angiogenesis following transcatheter arterial chemoembolization in rat hepatocellular carcinoma models.
In conclusion, ZA treatment enhanced the effects of TACE through inhibiting TAM infiltration and tumor angiogenesis in rat HCC models. PMID: 28943915 [PubMed] (Source: Oncology Letters)
Source: Oncology Letters - September 26, 2017 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Blockade of sonic hedgehog signaling decreases viability and induces apoptosis in retinoblastoma cells: The key role of the PI3K/Akt pathway.
Authors: Song Z, Du Y, Tao Y Abstract Retinoblastoma (RB) is the most common type of malignant intraocular cancer in teenagers. One of the proteins abnormally expressed during oncogenesis of RB is sonic hedgehog (SHH), which possesses the capability to selectively activate transcription factors of different genes. However, the detailed function of SHH in RB remains unknown. Thus, the present study sought to investigate the role of SHH in the development of RB. The human RB WERI-Rb-1 cell line was used as an in vitro model for the knockdown of SHH by a specific short hairpin RNA (shRNA). To assess the effect of SHH ...
Source: Oncology Letters - September 26, 2017 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Mechanisms of CXCR7 induction in malignant melanoma development.
Authors: Li XJ, Liu P, Tian WW, Li ZF, Liu BG, Sun JF Abstract Malignant melanoma (MM) is a highly malignant skin tumor. The mechanism of MM pathogenesis and its signaling pathways are not well characterized. C-X-C chemokine receptor type 7 (CXCR7) has been reported to regulate cancer cell invasion. The present study sought to investigate the effects of CXCR7 on MM development. First, CXCR7 expression levels were assessed in the skin tumor tissue of patients with MM. Then, CXCR7 small hairpin RNA was used in M14 melanoma cells in a Transwell culture model and in a transplanted mouse model to test the effects of CXC...
Source: Oncology Letters - September 26, 2017 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Effects of silenced PAR-2 on cell proliferation, invasion and metastasis of esophageal cancer.
Authors: Chen J, Xie L, Zheng Y, Liu C Abstract The present study aimed to investigate the effect of protease-activated receptor 2 (PAR-2) on cell proliferation, invasion and metastasis in the esophageal EC109 cell line. Two short hairpin RNA (shRNA) expression plasmids were constructed based on the PAR-2 mRNA sequence in humans, and they were transfected into the EC109 esophageal cancer cell line, and the stable interference cell line (shRNA-PAR-2 EC109) was obtained by puromycin selection. Following transfection of PAR-2 shRNA-1, PAR-2 expression was significantly downregulated in mRNA level and protein level in EC109 cells (P
Source: Oncology Letters - September 26, 2017 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

BRAF(V600E) mutation is not associated with central lymph node metastasis in all patients with papillary thyroid cancer: Different histological subtypes and preoperative lymph node status should be taken into account.
In conclusion, BRAF(V600E) was an independent risk factor for central LNM overall in patients with PTC and in patients with CVPTC, particularly in patients with cN0 CVPTC. However, BRAF(V600E) was not an independent risk factor for patients with FVPTC and AVPTC. Therefore, BRAF(V600E) provides varied clinical significance in different histological subtypes and preoperative central lymph node status. PMID: 28943919 [PubMed] (Source: Oncology Letters)
Source: Oncology Letters - September 26, 2017 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Clinical predictors of pathological complete response to neoadjuvant chemotherapy in triple-negative breast cancer.
Authors: Nakashoji A, Matsui A, Nagayama A, Iwata Y, Sasahara M, Murata Y Abstract The response of triple-negative breast cancer (TNBC) to chemotherapy is heterogeneous; particular subtype classifications based on mRNA gene expression analysis have been demonstrated to be associated with a pathological complete response (pCR). The aim of the present study was to investigate additional clinical and pathological characteristics associated with pCR status. The pathological and clinical characteristics of 40 TNBC patients who underwent neoadjuvant chemotherapy followed by surgery were retrospectively analyzed by dividi...
Source: Oncology Letters - September 26, 2017 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Safety and feasibility of laparoscopic intersphincteric resection for a lower rectal tumor.
Authors: Matsuhashi N, Takahashi T, Tanahashi T, Matsui S, Imai H, Tanaka Y, Yamaguchi K, Osada S, Yoshida K Abstract The aim of the present study was to evaluate the short-term surgical outcomes of laparoscopic intersphincteric resection (ISR) for a lower rectal tumor in comparison with a case-control series of patients undergoing open ISR. Quality of life factors and anal function were also evaluated. Between July 2008 and April 2013, 103 patients with lower rectal cancer underwent laparoscopic surgery at the Surgical Oncology Department of Gifu University School of Medicine. A total of 25 patients with lower rec...
Source: Oncology Letters - September 26, 2017 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Obesity accelerates murine gastric cancer growth by modulating the Sirt1/YAP pathway.
Authors: Li HJ, Fu JK, Che XM, Fan L, Zhang Y, Bai E Abstract A previous study from our group using an in vivo model demonstrated that diet induced-obesity increases the risk of gastric cancer and may prompt its growth. However, the molecular mechanisms underlying this association remain unclear and require further investigation. The aim of the present study was to investigate the potential molecular mechanisms through which obesity affects gastric cancer growth. In a subcutaneous mouse model, tumors were significantly larger in obese mice compared with non-obese and lean mice. In addition, markedly increased level...
Source: Oncology Letters - September 26, 2017 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Specific expression of proton-coupled oligopeptide transporter 1 in primary hepatocarcinoma-a novel strategy for tumor-targeted therapy.
In conclusion, our findings provided the novel information on the expression and function of PEPT1 in human hepatocarcinoma and expanded the potential values for tumor specific drug delivery. PMID: 28943923 [PubMed] (Source: Oncology Letters)
Source: Oncology Letters - September 26, 2017 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Network-based analysis of the molecular mechanisms of multiple myeloma and monoclonal gammopathy of undetermined significance.
Authors: Liu Z, Huang J, Zhong Q, She Y, Ou R, Li C, Chen R, Yao M, Zhang Q, Liu S Abstract The present study aimed to reveal the molecular mechanisms of multiple myeloma (MM) and monoclonal gammopathy of undetermined significance (MGUS). This was a secondary study on microarray dataset GSE80608, downloaded from the Gene Expression Omnibus database, which included 10 control samples, 10 MGUS samples and 10 MM samples. Differentially expressed genes (DEGs) were identified between control and MGUS samples, and between control and MM samples. A protein-protein interaction (PPI) network was built for studying the inter...
Source: Oncology Letters - September 26, 2017 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Genomic and immunohistochemical characterisation of a lacrimal gland oncocytoma and review of literature.
Authors: Mikkelsen LH, Andreasen S, Melchior LC, Persson M, Andersen JD, Pereira V, Toft PB, Morling N, Stenman G, Heegaard S Abstract The aim of the present study was to report the genetic and immunohistochemical profile of a rare case of lacrimal gland oncocytoma. A 20-year-old male underwent magnetic resonance imaging (MRI) due to viral encephalitis. Notably, the MRI revealed a multicystic tumor in the left lacrimal gland. A lateral orbitotomy was performed and the tumor was completely excised. Four months following surgery, the patient was free of symptoms. Histopathologically, the tumor was composed of large, ...
Source: Oncology Letters - September 26, 2017 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Combination of galectin-3, CK19 and HBME-1 immunostaining improves the diagnosis of thyroid cancer.
Authors: Arcolia V, Journe F, Renaud F, Leteurtre E, Gabius HJ, Remmelink M, Saussez S Abstract Currently, fine-needle aspiration is the most frequently used pre-operative technique for diagnosis of malignant thyroid tumors, however, pathologists are unable to reach efficient and accurate differential diagnoses between benign and malignant thyroid nodules. To aid in resolving this issue, immunohistochemistry for galectins (gal)-1, -3, -7, -8, cytokeratin 19 (CK19), Hector Battifora Mesothelial Epitope-1 (HBME-1) and thyroid peroxidase (TPO) was performed on two tissue microarrays composed of 66 follicular adenomas ...
Source: Oncology Letters - September 26, 2017 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Measurement of exhaled nitric oxide and serum surfactant protein D levels for monitoring radiation pneumonitis following thoracic radiotherapy.
In conclusion, obtaining the eNO ratio was a useful RP monitoring tool but did not predict RP occurrence in the present setting, whereas serum SP-D level may be a potential predictor for the detection of RP risk. PMID: 28943927 [PubMed] (Source: Oncology Letters)
Source: Oncology Letters - September 26, 2017 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

RecQ helicase BLM regulates prostate cancer cell proliferation and apoptosis.
Authors: Qian X, Feng S, Xie D, Feng D, Jiang Y, Zhang X Abstract Prostate cancer (PCa) is a common malignant tumor and the second leading cause of morbidity and mortality in men worldwide. Considering the prevalence and effects of PCa in males, an understanding of the molecular mechanisms underlying PCa tumorigenesis are essential and may provide novel therapeutic strategies for treating PCa. Bloom syndrome protein (BLM) is a member of the RecQ helicase family. The major function of BLM is to uncoil the double-stranded DNA structure. It has previously been demonstrated that BLM acts as a 'genome caretaker', and dy...
Source: Oncology Letters - September 26, 2017 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Contrast-enhanced multiple-phase imaging features of intrahepatic mass-forming cholangiocarcinoma and hepatocellular carcinoma with cirrhosis: A comparative study.
Authors: Chen Y, Pan Y, Shen KR, Zhu XL, Lu CY, Li QH, Han SG, Fu YB, Xu XF, Yu RS Abstract The intrahepatic mass-forming cholangiocarcinoma (IMCC) is frequently misdiagnosed as hepatocellular carcinoma (HCC) in patients with cirrhosis, by numerous radiologists and clinical doctors, which results in the incorrect therapeutic treatment. A retrospective case-control study was conducted, and the contrast-enhanced multiple-phase (CEMP) computed tomography (CT) and magnetic resonance imaging (MRI) findings of 22 pathologically confirmed IMCC patients and 22 HCC controls with underlying liver cirrhosis were analyzed at t...
Source: Oncology Letters - September 26, 2017 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Downregulation of cytochrome c oxidase 1 induced radioresistance in esophageal squamous cell carcinoma.
Authors: Takesue T, Kawakubo H, Hayashida T, Tsutsui M, Miyao K, Fukuda K, Nakamura R, Takahashi T, Wada N, Takeuchi H, Kitagawa Y Abstract Comprehensive gene screening with transposons is a novel procedure for the systematic identification of resistant genes. The present study aimed to use this technique to identify candidate radioresistant genes in esophageal squamous cell carcinoma. A transposon is a base sequence that can translocate to another location in the genome at random. By inserting the cytomegalovirus promotor as a transcriptional activator in the transposon, the following gene in the new location beco...
Source: Oncology Letters - September 26, 2017 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

A missing link between RON expression and oncological outcomes in resected left-sided pancreatic cancer.
Authors: Han DH, Kang CM, Lee SW, Hwang HK, Lee WJ Abstract Alteration and activation of recepteur d'origine nantais (RON) expression is known to be associated with cancer progression and decreased survival in various types of human cancer, including pancreatic cancer. Therefore, in the present study, RON expression levels were determined in resected left-sided pancreatic cancer to evaluate the potential oncological role of RON in the clinical setting of distal pancreatic cancer. From January 2005 to December 2011, a total of 57 patients underwent radical distal pancreatectomy for left-sided pancreatic cancer. Duct...
Source: Oncology Letters - September 26, 2017 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research