A cell-free multi-enzyme cascade for the synthesis of CDP-glycerol
Chembiochem. 2023 Aug 14:e202300463. doi: 10.1002/cbic.202300463. Online ahead of print.ABSTRACTCDP-glycerol is a nucleotide-diphosphate-activated version of glycerol. In nature, it is required for the biosynthesis of teichoic acid in Gram-positive bacteria, which is an appealing target epitope for the development of new vaccines. Here, a cell-free multi-enzyme cascade was developed to synthetize nucleotide-activated glycerol from the inexpensive and readily available substrates cytidine and glycerol. The cascade comprises five recombinant enzymes expressed in E. coli that were purified by immobilized metal affinity chroma...
Source: Chembiochem - August 14, 2023 Category: Biochemistry Authors: E Alberto Alcal á-Orozco Valerian Grote Timm Fiebig Steffen Klamt Udo Reichl Thomas F T Rexer Source Type: research
A cell-free multi-enzyme cascade for the synthesis of CDP-glycerol
Chembiochem. 2023 Aug 14:e202300463. doi: 10.1002/cbic.202300463. Online ahead of print.ABSTRACTCDP-glycerol is a nucleotide-diphosphate-activated version of glycerol. In nature, it is required for the biosynthesis of teichoic acid in Gram-positive bacteria, which is an appealing target epitope for the development of new vaccines. Here, a cell-free multi-enzyme cascade was developed to synthetize nucleotide-activated glycerol from the inexpensive and readily available substrates cytidine and glycerol. The cascade comprises five recombinant enzymes expressed in E. coli that were purified by immobilized metal affinity chroma...
Source: Chembiochem - August 14, 2023 Category: Biochemistry Authors: E Alberto Alcal á-Orozco Valerian Grote Timm Fiebig Steffen Klamt Udo Reichl Thomas F T Rexer Source Type: research
IJERPH, Vol. 20, Pages 6551: Safety Concerns Related to the Simultaneous Use of Prescription or Over-the-Counter Medications and Herbal Medicinal Products: Survey Results among Latvian Citizens
Ardava
The use of herbal medicines is increasing worldwide. While the safety profile of many herbal medicines is promising, the data in the literature show important interactions with conventional drugs that can expose individual patients to high risk. The aim of this study was to investigate the experience of the use of herbal medicines and preparations and the risks of interactions between herbal and conventional medicines among Latvian citizens. Data were collected between 2019 and 2021 using a structured questionnaire designed for pharmacy customers in Latvia. Electronic databases such as Drugs.com, Medscape, and ...
Source: International Journal of Environmental Research and Public Health - August 9, 2023 Category: Environmental Health Authors: Inga Sile Renate Teterovska Oskars Onzevs Elita Ardava Tags: Article Source Type: research
Complementary and alternative treatments for insomnia disorder: a systematic umbrella review
J Sleep Res. 2023 Aug 1:e13979. doi: 10.1111/jsr.13979. Online ahead of print.ABSTRACTInsomnia is a common disorder and cognitive behavioural therapy for insomnia (CBT-I) is recommended as first-line treatment. However, CBT-I is not widely distributed and infrequently available while medication is not indicated for long-term use. To close this evident gap in supply, alternative treatments could be utilised. High-quality research on this topic is scarce, and there is currently no comprehensive publication on the effectiveness of alternative treatments. To address this pressing question, we systematically summarised the exis...
Source: Journal of Sleep Research - August 1, 2023 Category: Sleep Medicine Authors: Johanna Ell Sarah R Schmid Fee Benz Lukas Spille Source Type: research
Complementary and alternative treatments for insomnia disorder: a systematic umbrella review
J Sleep Res. 2023 Aug 1:e13979. doi: 10.1111/jsr.13979. Online ahead of print.ABSTRACTInsomnia is a common disorder and cognitive behavioural therapy for insomnia (CBT-I) is recommended as first-line treatment. However, CBT-I is not widely distributed and infrequently available while medication is not indicated for long-term use. To close this evident gap in supply, alternative treatments could be utilised. High-quality research on this topic is scarce, and there is currently no comprehensive publication on the effectiveness of alternative treatments. To address this pressing question, we systematically summarised the exis...
Source: Journal of Sleep Research - August 1, 2023 Category: Sleep Medicine Authors: Johanna Ell Sarah R Schmid Fee Benz Lukas Spille Source Type: research
Molecules, Vol. 28, Pages 5726: Tag and Snag: A New Platform for Bioactive Natural Product Screening from Mixtures
Wenjun Zhang
Natural products provide an unparalleled diversity of small molecules to fuel drug screening efforts, but deconvoluting the pharmacological activity of natural product mixtures to identify key bioactive compounds remains a vexing and labor-intensive process. Therefore, we have developed a new platform to probe the non-specific pharmacological potential of compounds present in common dietary supplements via shotgun derivatization with isotopically labeled propanoic acid, a live cell affinity assay, which was used to selectively recognize the population of compounds which bind tightly to HeLa cells in cul...
Source: Molecules - July 28, 2023 Category: Chemistry Authors: Jeremy Seidel Yongle Du Rohin Devanathan Richard Law Zhijuan Hu Nicholas A. Zill Anthony T. Iavarone Wenjun Zhang Tags: Article Source Type: research
The impact of competing stroke etiologies in patients with atrial fibrillation
CONCLUSION: In stroke patients with AF, causes other than cardioembolism as competing etiologies were common in index or recurrent IS. Concomitant presence of large-artery-atherosclerosis seems to indicate an increased risk for recurrences suggesting that stroke preventive means might be more effective if they also address competing stroke etiologies in AF-stroke patients.CLINICAL TRIAL REGISTRATION: NCT03826927.PMID:37401394 | DOI:10.1177/23969873231185220 (Source: Atherosclerosis)
Source: Atherosclerosis - July 4, 2023 Category: Cardiology Authors: Annaelle Zietz Alexandros A Polymeris Fabrice Helfenstein Sabine Schaedelin Lisa Hert Benjamin Wagner David J Seiffge Christopher Traenka Valerian L Altersberger Tolga Dittrich Josefin Kaufmann Flavia Ravanelli Joachim Fladt Urs Fisch Sebastian Thilemann Source Type: research
The impact of competing stroke etiologies in patients with atrial fibrillation
CONCLUSION: In stroke patients with AF, causes other than cardioembolism as competing etiologies were common in index or recurrent IS. Concomitant presence of large-artery-atherosclerosis seems to indicate an increased risk for recurrences suggesting that stroke preventive means might be more effective if they also address competing stroke etiologies in AF-stroke patients.CLINICAL TRIAL REGISTRATION: NCT03826927.PMID:37401394 | DOI:10.1177/23969873231185220 (Source: Atherosclerosis)
Source: Atherosclerosis - July 4, 2023 Category: Cardiology Authors: Annaelle Zietz Alexandros A Polymeris Fabrice Helfenstein Sabine Schaedelin Lisa Hert Benjamin Wagner David J Seiffge Christopher Traenka Valerian L Altersberger Tolga Dittrich Josefin Kaufmann Flavia Ravanelli Joachim Fladt Urs Fisch Sebastian Thilemann Source Type: research
Plant virus movement proteins originated from jelly-roll capsid proteins
by Anamarija Butkovic, Valerian V. Dolja, Eugene V. Koonin, Mart Krupovic
Numerous, diverse plant viruses encode movement proteins (MPs) that aid the virus movement through plasmodesmata, the plant intercellular channels. MPs are essential for virus spread and propagation in distal tissues, and several unrelated MPs have been identified. The 30K superfamily of MPs (named after the molecular mass of tobacco mosaic virus (TMV) MP, the classical model of plant virology) is the largest and most diverse MP variety, represented in 16 virus families, but its evolutionary origin remained obscure. Here, we show that the core struc...
Source: PLoS Biology: Archived Table of Contents - June 15, 2023 Category: Biology Authors: Anamarija Butkovic Source Type: research
Impact, cost-effectiveness, and budget implications of HPV vaccination in Kenya: A modelling study
CONCLUSION: HPV vaccination for girls is highly cost-effective in Kenya. Compared to GARDASIL-4, alternative products could provide similar or greater health benefits at lower net costs. Substantial government funding will be required to reach and sustain coverage targets as Kenya graduates from Gavi support. A single dose strategy is likely to have similar benefits for less cost.PMID:37296015 | DOI:10.1016/j.vaccine.2023.05.019 (Source: Vaccine)
Source: Vaccine - June 9, 2023 Category: Allergy & Immunology Authors: Valerian Mwenda Rose Jalang'o Christine Miano Joan-Paula Bor Mary Nyangasi Lucy Mecca Vincent Were Edward Kariithi Clint Pecenka Anne Schuind Kaja Abbas Andrew Clark Source Type: research
Impact, cost-effectiveness, and budget implications of HPV vaccination in Kenya: A modelling study
CONCLUSION: HPV vaccination for girls is highly cost-effective in Kenya. Compared to GARDASIL-4, alternative products could provide similar or greater health benefits at lower net costs. Substantial government funding will be required to reach and sustain coverage targets as Kenya graduates from Gavi support. A single dose strategy is likely to have similar benefits for less cost.PMID:37296015 | DOI:10.1016/j.vaccine.2023.05.019 (Source: Vaccine)
Source: Vaccine - June 9, 2023 Category: Allergy & Immunology Authors: Valerian Mwenda Rose Jalang'o Christine Miano Joan-Paula Bor Mary Nyangasi Lucy Mecca Vincent Were Edward Kariithi Clint Pecenka Anne Schuind Kaja Abbas Andrew Clark Source Type: research
Integrative medicine, naturopathic and complementary medicine in the treatment of fibromyalgia syndrome : An overview against the background of clinical experiences from routine treatment
Schmerz. 2023 May 22. doi: 10.1007/s00482-023-00716-7. Online ahead of print.ABSTRACTFibromyalgia syndrome is classified as a functional somatic syndrome. There are typical but not clearly defined symptom clusters, such as chronic widespread pain, non-restorative sleep, and a tendency to physical and/or mental exhaustion. The core principle of treatment in the S3 guidelines is a multimodal treatment, especially for severe forms of the disease. Complementary, naturopathic and integrative forms of treatment have an established place in the guidelines. Strong treatment recommendations with a high consensus exist for endurance...
Source: Schmerz - May 22, 2023 Category: Anesthesiology Authors: Andrea Langhorst Jost Langhorst Source Type: research
Integrative medicine, naturopathic and complementary medicine in the treatment of fibromyalgia syndrome : An overview against the background of clinical experiences from routine treatment
Schmerz. 2023 May 22. doi: 10.1007/s00482-023-00716-7. Online ahead of print.ABSTRACTFibromyalgia syndrome is classified as a functional somatic syndrome. There are typical but not clearly defined symptom clusters, such as chronic widespread pain, non-restorative sleep, and a tendency to physical and/or mental exhaustion. The core principle of treatment in the S3 guidelines is a multimodal treatment, especially for severe forms of the disease. Complementary, naturopathic and integrative forms of treatment have an established place in the guidelines. Strong treatment recommendations with a high consensus exist for endurance...
Source: Schmerz - May 22, 2023 Category: Anesthesiology Authors: Andrea Langhorst Jost Langhorst Source Type: research
Integrative medicine, naturopathic and complementary medicine in the treatment of fibromyalgia syndrome : An overview against the background of clinical experiences from routine treatment
Schmerz. 2023 May 22. doi: 10.1007/s00482-023-00716-7. Online ahead of print.ABSTRACTFibromyalgia syndrome is classified as a functional somatic syndrome. There are typical but not clearly defined symptom clusters, such as chronic widespread pain, non-restorative sleep, and a tendency to physical and/or mental exhaustion. The core principle of treatment in the S3 guidelines is a multimodal treatment, especially for severe forms of the disease. Complementary, naturopathic and integrative forms of treatment have an established place in the guidelines. Strong treatment recommendations with a high consensus exist for endurance...
Source: Schmerz - May 22, 2023 Category: Anesthesiology Authors: Andrea Langhorst Jost Langhorst Source Type: research
Integrative medicine, naturopathic and complementary medicine in the treatment of fibromyalgia syndrome : An overview against the background of clinical experiences from routine treatment
Schmerz. 2023 May 22. doi: 10.1007/s00482-023-00716-7. Online ahead of print.ABSTRACTFibromyalgia syndrome is classified as a functional somatic syndrome. There are typical but not clearly defined symptom clusters, such as chronic widespread pain, non-restorative sleep, and a tendency to physical and/or mental exhaustion. The core principle of treatment in the S3 guidelines is a multimodal treatment, especially for severe forms of the disease. Complementary, naturopathic and integrative forms of treatment have an established place in the guidelines. Strong treatment recommendations with a high consensus exist for endurance...
Source: Schmerz - May 22, 2023 Category: Anesthesiology Authors: Andrea Langhorst Jost Langhorst Source Type: research
