Amplification- and Enzyme-Free Magnetic Diagnostics Circuit for Whole-Genome Detection of SARS-CoV-2 RNA
Chembiochem. 2024 May 6:e202400251. doi: 10.1002/cbic.202400251. Online ahead of print.ABSTRACTPolymerase chain reaction (PCR) requires thermal cycling and enzymatic reactions for sequence amplification, hampering their applications in point-of-care (POC) settings. Magnetic bioassays based on magnetic particle spectroscopy (MPS) and magnetic nanoparticles (MNPs) are isothermal, wash-free, and can be quantitative. Realizing them amplification- and enzyme-free on a benchtop device, they will become irreplaceable for POC applications. Here we demonstrate a first-in-class magnetic signal amplification circuit (MAC) that enable...
Source: Chembiochem - May 6, 2024 Category: Biochemistry Authors: Enja Laureen R ösch Rebecca Sack Mohammad Suman Chowdhury Florian Wolgast Margarete Zaborski Frank Ludwig Meinhard Schilling Thilo Viereck Ulfert Rand Aidin Lak Source Type: research
Inhibition of RNA Phosphodiester Backbone Cleavage in the Presence of Organic Cations of Different Sizes
Chembiochem. 2024 May 6:e202400276. doi: 10.1002/cbic.202400276. Online ahead of print.ABSTRACTLiving cells contain various types of organic cations that may interact with nucleic acids. In order to understand the nucleic acid-binding properties of organic cations of different sizes, we investigated the ability of simple organic cations to inhibit the RNA phosphodiester bond cleavage promoted by Mg2+, Pb2+, and RNA-cleaving serum proteins. Kinetic analysis using chimeric DNA-RNA oligonucleotides showed that the cleavage at ribonucleotide sites was inhibited in the presence of monovalent cations comprising alkyl chains or b...
Source: Chembiochem - May 6, 2024 Category: Biochemistry Authors: Senri Yoshioka Asuka Doi Shu-Ichi Nakano Source Type: research
Probing Glycerolipid Metabolism using a Caged Clickable Glycerol-3-Phosphate Probe
In this study, we present the probe SATE-G3P-N3 as a novel tool for metabolic labeling of glycerolipids (GLs) to investigate lipid metabolism in yeast cells. By introducing a clickable azide handle onto the glycerol backbone, this probe enables general labeling of glycerolipids. Additionally, this probe contains a caged phosphate moiety at the glycerol sn-3 position to not only facilitate probe uptake by masking negative charge but also to bypass the phosphorylation step crucial for initiating phospholipid synthesis, thereby enhancing phospholipid labeling. The metabolic labeling activity of the probe was thoroughly assess...
Source: Chembiochem - May 5, 2024 Category: Biochemistry Authors: Jinchao Lou Christelle F Ancajas Yue Zhou Nicolas S Lane Todd B Reynolds Michael D Best Source Type: research
Oligonucleotide-Based Photoaffinity Probes: Chemical Tools and Applications for Protein Labeling
Chembiochem. 2024 May 4:e202400097. doi: 10.1002/cbic.202400097. Online ahead of print.ABSTRACTA variety of proteins interact with DNA and RNA, including polymerases, histones, ribosomes, transcription factors, and repair enzymes. However, the transient non-covalent nature of these interactions poses challenges for analysis. Introducing a covalent bond between proteins and DNA via photochemical activation of a photosensitive functional group introduced onto nucleic acids offers a means to stabilize these often weak interactions without significantly altering the binding interface. Consequently, photoactivatable oligonucleo...
Source: Chembiochem - May 4, 2024 Category: Biochemistry Authors: Carole Saintom é Oc éane Monfret Gilles Doisneau Dominique Guianvarc'h Source Type: research
Oligonucleotide-Based Photoaffinity Probes: Chemical Tools and Applications for Protein Labeling
Chembiochem. 2024 May 4:e202400097. doi: 10.1002/cbic.202400097. Online ahead of print.ABSTRACTA variety of proteins interact with DNA and RNA, including polymerases, histones, ribosomes, transcription factors, and repair enzymes. However, the transient non-covalent nature of these interactions poses challenges for analysis. Introducing a covalent bond between proteins and DNA via photochemical activation of a photosensitive functional group introduced onto nucleic acids offers a means to stabilize these often weak interactions without significantly altering the binding interface. Consequently, photoactivatable oligonucleo...
Source: Chembiochem - May 4, 2024 Category: Biochemistry Authors: Carole Saintom é Oc éane Monfret Gilles Doisneau Dominique Guianvarc'h Source Type: research
Multifunctional DNA Nanoflower Applied for High Specific Photodynamic Cancer Therapy in vivo
Chembiochem. 2024 May 3:e202400229. doi: 10.1002/cbic.202400229. Online ahead of print.ABSTRACTPhotodynamic therapy (PDT) is a newly emerged strategy for disease treatment. One challenge of the application of PDT drugs is the side-effect caused by the non-specificity of the photosensitive molecules. Most of the photosensitizers may invade not only the pathogenic cells but also the normal cells. In recent, people tried to use special cargoes to deliver the drugs into target cells. DNA nanoflowers (NFs) are a kind of newly-emerged nanomaterial which constructed through DNA rolling cycle amplification (RCA) reaction. It is re...
Source: Chembiochem - May 3, 2024 Category: Biochemistry Authors: Hao Zheng Xue-Nan Feng Xiang-Wan-Er Jin Zhi-Qi Dai Sha Lu Yun-Xi Cui De Kong Source Type: research
Functionalized DNA Origami-Enabled Detection of Biomarkers
Chembiochem. 2024 May 3:e202400227. doi: 10.1002/cbic.202400227. Online ahead of print.ABSTRACTBiomarkers are crucial physiological and pathological indicators in the host. Over the years, numerous detection methods have been developed for biomarkers, given their significant potential in various biological and biomedical applications. Among these, the detection system based on functionalized DNA origami has emerged as a promising approach due to its precise control over sensing modules, enabling sensitive, specific, and programmable biomarker detection. We summarize the advancements in biomarker detection using functionali...
Source: Chembiochem - May 3, 2024 Category: Biochemistry Authors: Caiqing Yuan Fei Zhou Zhihao Xu Dunkai Wu Pengfei Hou Donglei Yang Li Pan Pengfei Wang Source Type: research
Using LanM Enzymes to Modify Glycagon-Like Peptides 1 and 2 in E.coli
Chembiochem. 2024 May 3:e202400201. doi: 10.1002/cbic.202400201. Online ahead of print.ABSTRACTSelective modification of peptides is often exploited to improve pharmaceutically relevant properties of bioactive peptides like stability, circulation time, and potency. In Nature, natural products belonging to the class of ribosomally synthesized and post-translationally modified peptides (RiPPs) are known to install a number of highly attractive modifications with high selectivity. These modifications are installed by enzymes guided to the peptide by corresponding leader peptides that are removed as the last step of biosynthes...
Source: Chembiochem - May 3, 2024 Category: Biochemistry Authors: Camilla Kjeldgaard Larsen Peter Lindquist Mette Rosenkilde Alice Ravn Madsen Kim Haselmann Tine Glendorf Kjeld Olesen Anne Louise Bank Kodal Thomas T ørring Source Type: research
Multifunctional DNA Nanoflower Applied for High Specific Photodynamic Cancer Therapy in vivo
Chembiochem. 2024 May 3:e202400229. doi: 10.1002/cbic.202400229. Online ahead of print.ABSTRACTPhotodynamic therapy (PDT) is a newly emerged strategy for disease treatment. One challenge of the application of PDT drugs is the side-effect caused by the non-specificity of the photosensitive molecules. Most of the photosensitizers may invade not only the pathogenic cells but also the normal cells. In recent, people tried to use special cargoes to deliver the drugs into target cells. DNA nanoflowers (NFs) are a kind of newly-emerged nanomaterial which constructed through DNA rolling cycle amplification (RCA) reaction. It is re...
Source: Chembiochem - May 3, 2024 Category: Biochemistry Authors: Hao Zheng Xue-Nan Feng Xiang-Wan-Er Jin Zhi-Qi Dai Sha Lu Yun-Xi Cui De Kong Source Type: research
Functionalized DNA Origami-Enabled Detection of Biomarkers
Chembiochem. 2024 May 3:e202400227. doi: 10.1002/cbic.202400227. Online ahead of print.ABSTRACTBiomarkers are crucial physiological and pathological indicators in the host. Over the years, numerous detection methods have been developed for biomarkers, given their significant potential in various biological and biomedical applications. Among these, the detection system based on functionalized DNA origami has emerged as a promising approach due to its precise control over sensing modules, enabling sensitive, specific, and programmable biomarker detection. We summarize the advancements in biomarker detection using functionali...
Source: Chembiochem - May 3, 2024 Category: Biochemistry Authors: Caiqing Yuan Fei Zhou Zhihao Xu Dunkai Wu Pengfei Hou Donglei Yang Li Pan Pengfei Wang Source Type: research
Using LanM Enzymes to Modify Glycagon-Like Peptides 1 and 2 in E.coli
Chembiochem. 2024 May 3:e202400201. doi: 10.1002/cbic.202400201. Online ahead of print.ABSTRACTSelective modification of peptides is often exploited to improve pharmaceutically relevant properties of bioactive peptides like stability, circulation time, and potency. In Nature, natural products belonging to the class of ribosomally synthesized and post-translationally modified peptides (RiPPs) are known to install a number of highly attractive modifications with high selectivity. These modifications are installed by enzymes guided to the peptide by corresponding leader peptides that are removed as the last step of biosynthes...
Source: Chembiochem - May 3, 2024 Category: Biochemistry Authors: Camilla Kjeldgaard Larsen Peter Lindquist Mette Rosenkilde Alice Ravn Madsen Kim Haselmann Tine Glendorf Kjeld Olesen Anne Louise Bank Kodal Thomas T ørring Source Type: research
Synthesis of new bodipy hydrazide fluorescent probes for the detection of carbonylated proteins generated by oxidative stress
We describe the design and the synthetic pathway of new BODIPY fluorescent probes functionalized with hydrazide function for protein carbonyl labeling to improve existing methodologies such as 2D-Oxi electrophoresis. Hydrazide BODIPY analogues show very good fluorescent properties such as NIR emission up to 633 nm and quantum yield up to 0.88. These new probes were validated for the detection and quantification of carbonylated proteins with 2D-Oxi electrophoresis using mouse muscle protein extracts, as well as both flow cytometry and microscopy using oxidant stressed C2C12 cells.PMID:38695553 | DOI:10.1002/cbic.202400093 (Source: Chembiochem)
Source: Chembiochem - May 2, 2024 Category: Biochemistry Authors: Anthony Nina Diogo Janek Hyzewicz Marie-Paule Hamon Jeremy Fort é Serge Thorimbert Bertrand Friguet Candice Botuha Source Type: research
PET Ligands for Imaging Mutant Huntingtin Aggregates: A Case Study in Non-For-Profit Scientific Management
Chembiochem. 2024 May 2:e202400152. doi: 10.1002/cbic.202400152. Online ahead of print.ABSTRACTPositron emission tomography imaging of misfolded proteins with high-affinity and selective radioligands has played a vital role in expanding our knowledge of neurodegenerative diseases such as Parkinson's and Alzheimer's disease. The pathogenesis of Huntington's disease, a CAG trinucleotide repeat disorder, is similarly linked to the presence of protein fibrils formed from mutant huntingtin (mHTT) protein. Development of mHTT fibril-specific radioligands has been limited by the lack of structural knowledge around mHTT and a dear...
Source: Chembiochem - May 2, 2024 Category: Biochemistry Authors: Catherine G F Dickmann Selena Milicevic Sephton Roger A Barker Franklin I Aigbirhio Source Type: research
Improvement of heterologous soluble expression of L-amino acid oxidase using logistic regression
In this study, we present a new and more accurate model for predicting amino acids associated with an increased L-amino acid oxidase (LAO) solubility. Expressing LAO from Rhizoctonia solani in Escherichia coli and combining random mutagenesis and statistical logistic regression, we modified 108 amino acid residues by substituting hydrophobic amino acids with serine and hydrophilic amino acids with alanine. Our results indicated that specific mutations in Euclidean distance, glycine, methionine, and secondary structure increased LAO expression. Furthermore, repeated mutations were performed for LAO based on logistic regress...
Source: Chembiochem - May 2, 2024 Category: Biochemistry Authors: Ayuta Nakahara Zhengyu Su Mamoru Wakayama Masaki Nakamura Kazutoshi Sakakibara Daisuke Matsui Source Type: research
Synthesis of new bodipy hydrazide fluorescent probes for the detection of carbonylated proteins generated by oxidative stress
We describe the design and the synthetic pathway of new BODIPY fluorescent probes functionalized with hydrazide function for protein carbonyl labeling to improve existing methodologies such as 2D-Oxi electrophoresis. Hydrazide BODIPY analogues show very good fluorescent properties such as NIR emission up to 633 nm and quantum yield up to 0.88. These new probes were validated for the detection and quantification of carbonylated proteins with 2D-Oxi electrophoresis using mouse muscle protein extracts, as well as both flow cytometry and microscopy using oxidant stressed C2C12 cells.PMID:38695553 | DOI:10.1002/cbic.202400093 (Source: Chembiochem)
Source: Chembiochem - May 2, 2024 Category: Biochemistry Authors: Anthony Nina Diogo Janek Hyzewicz Marie-Paule Hamon Jeremy Fort é Serge Thorimbert Bertrand Friguet Candice Botuha Source Type: research
