Illuminating the Path to Enhanced Bioimaging by Phosphole-based Fluorophores
In this study, we introduce phosphole-based fluorophores that surpass existing options like dansyl chloride. The reactive S-Cl bond in chlorosulfonylimino-5-phenylphosphole derivatives allows rapid and direct coupling to peptides making the fluorophores easily introducible to peptides. This coupling process occurs under mild conditions, demonstrated for [F7 ,P34 ]-NPY and its shorter analogues. Peptides linked with our fluorophores exhibit similar receptor activation to the control peptide, while maintaining high stability and low toxicity, making them ideal biolabeling reagents. In fluorescence microscopy experiments, the...
Source: Chembiochem - January 11, 2024 Category: Biochemistry Authors: Mareike Schenk Nils K önig Evamarie Hey-Hawkins Annette G Beck-Sickinger Source Type: research
Design and Evaluation of PROTACs Targeting Acyl Protein Thioesterase 1
Chembiochem. 2024 Jan 9:e202300736. doi: 10.1002/cbic.202300736. Online ahead of print.ABSTRACTPROTAC linker design remains mostly an empirical task. We employed the PRosettaC computational software in the design of sulfonyl-fluoride-based PROTACs targeting acyl protein thioesterase 1 (APT1). The software efficiently generated ternary complex models from empirically-designed PROTACs and suggested alkyl linkers to be the preferred type of linker to target APT1. Western blotting analysis revealed efficient degradation of APT1 and activity-based protein profiling showed remarkable selectivity of an alkyl linker-based PROTAC a...
Source: Chembiochem - January 10, 2024 Category: Biochemistry Authors: Lu ís A R Carvalho B árbara B Sousa Daniel Zaidman Hannah Kiely-Collins Gon çalo J L Bernardes Source Type: research
In-Bridge Stereochemistry: A Determinant of Stapled Peptide Conformation and Activity
Chembiochem. 2024 Jan 8:e202300747. doi: 10.1002/cbic.202300747. Online ahead of print.ABSTRACTPeptide side chain stapling has been proven to be an effective strategy for fine-tuning peptide properties. This innovative approach leads to the creation of stapled peptides characterized by stabilized α-helical conformations, enhanced protein-binding affinity, improved cell permeability, superior enzymatic stability, and numerous other advantages. Extensive research has explored the impact of various stapling bridges on the properties of these peptides, with limited investigation into the influence of bridge chirality, until v...
Source: Chembiochem - January 9, 2024 Category: Biochemistry Authors: Jingyi Zhang Suwei Dong Source Type: research
In-Bridge Stereochemistry: A Determinant of Stapled Peptide Conformation and Activity
Chembiochem. 2024 Jan 8:e202300747. doi: 10.1002/cbic.202300747. Online ahead of print.ABSTRACTPeptide side chain stapling has been proven to be an effective strategy for fine-tuning peptide properties. This innovative approach leads to the creation of stapled peptides characterized by stabilized α-helical conformations, enhanced protein-binding affinity, improved cell permeability, superior enzymatic stability, and numerous other advantages. Extensive research has explored the impact of various stapling bridges on the properties of these peptides, with limited investigation into the influence of bridge chirality, until v...
Source: Chembiochem - January 9, 2024 Category: Biochemistry Authors: Jingyi Zhang Suwei Dong Source Type: research
A facile and general tandem oligonucleotide synthesis methodology for DNA and RNA
Chembiochem. 2024 Jan 5:e202300870. doi: 10.1002/cbic.202300870. Online ahead of print.ABSTRACTTandem oligonucleotide synthesis (TOS) is an attractive strategy to increase automated oligonucleotide synthesis efficiency. TOS is accomplished via the introduction of an immolative linker within a single sequence composed of multiple oligonucleotide fragments. Here, we report the use of a commercially available building block, typically utilized for the chemical phosphorylation of DNA/RNA oligomers, to perform TOS. We show that the 2,2'-sulfonyldiethylene linker is efficiently self-immolated during the standard deprotection of ...
Source: Chembiochem - January 5, 2024 Category: Biochemistry Authors: Jagandeep S Saraya Derek O'Flaherty Source Type: research
Selection and Characterization of DNA Aptamers for Cytidine and Uridine
This study employs the library-immobilization capture-SELEX technique to isolate aptamers capable of selectively binding to cytidine and uridine. First, a selection was performed using a mixture of cytidine and uridine as the target. This selection led to the isolation of a highly selective aptamer for cytidine with a dissociation constant (Kd ) of 0.9 μM as determined by isothermal titration calorimetry (ITC). In addition, a dual-recognition aptamer was also discovered, which exhibited selective binding to both cytidine and uridine. Subsequently, a separate selection was carried out using uridine as the sole target, and ...
Source: Chembiochem - January 5, 2024 Category: Biochemistry Authors: Lide Gu Jiajie Zheng Yao Zhang Deli Wang Juewen Liu Source Type: research
A facile and general tandem oligonucleotide synthesis methodology for DNA and RNA
Chembiochem. 2024 Jan 5:e202300870. doi: 10.1002/cbic.202300870. Online ahead of print.ABSTRACTTandem oligonucleotide synthesis (TOS) is an attractive strategy to increase automated oligonucleotide synthesis efficiency. TOS is accomplished via the introduction of an immolative linker within a single sequence composed of multiple oligonucleotide fragments. Here, we report the use of a commercially available building block, typically utilized for the chemical phosphorylation of DNA/RNA oligomers, to perform TOS. We show that the 2,2'-sulfonyldiethylene linker is efficiently self-immolated during the standard deprotection of ...
Source: Chembiochem - January 5, 2024 Category: Biochemistry Authors: Jagandeep S Saraya Derek O'Flaherty Source Type: research
Selection and Characterization of DNA Aptamers for Cytidine and Uridine
This study employs the library-immobilization capture-SELEX technique to isolate aptamers capable of selectively binding to cytidine and uridine. First, a selection was performed using a mixture of cytidine and uridine as the target. This selection led to the isolation of a highly selective aptamer for cytidine with a dissociation constant (Kd ) of 0.9 μM as determined by isothermal titration calorimetry (ITC). In addition, a dual-recognition aptamer was also discovered, which exhibited selective binding to both cytidine and uridine. Subsequently, a separate selection was carried out using uridine as the sole target, and ...
Source: Chembiochem - January 5, 2024 Category: Biochemistry Authors: Lide Gu Jiajie Zheng Yao Zhang Deli Wang Juewen Liu Source Type: research
A facile and general tandem oligonucleotide synthesis methodology for DNA and RNA
Chembiochem. 2024 Jan 5:e202300870. doi: 10.1002/cbic.202300870. Online ahead of print.ABSTRACTTandem oligonucleotide synthesis (TOS) is an attractive strategy to increase automated oligonucleotide synthesis efficiency. TOS is accomplished via the introduction of an immolative linker within a single sequence composed of multiple oligonucleotide fragments. Here, we report the use of a commercially available building block, typically utilized for the chemical phosphorylation of DNA/RNA oligomers, to perform TOS. We show that the 2,2'-sulfonyldiethylene linker is efficiently self-immolated during the standard deprotection of ...
Source: Chembiochem - January 5, 2024 Category: Biochemistry Authors: Jagandeep S Saraya Derek O'Flaherty Source Type: research
Selection and Characterization of DNA Aptamers for Cytidine and Uridine
This study employs the library-immobilization capture-SELEX technique to isolate aptamers capable of selectively binding to cytidine and uridine. First, a selection was performed using a mixture of cytidine and uridine as the target. This selection led to the isolation of a highly selective aptamer for cytidine with a dissociation constant (Kd ) of 0.9 μM as determined by isothermal titration calorimetry (ITC). In addition, a dual-recognition aptamer was also discovered, which exhibited selective binding to both cytidine and uridine. Subsequently, a separate selection was carried out using uridine as the sole target, and ...
Source: Chembiochem - January 5, 2024 Category: Biochemistry Authors: Lide Gu Jiajie Zheng Yao Zhang Deli Wang Juewen Liu Source Type: research
A facile and general tandem oligonucleotide synthesis methodology for DNA and RNA
Chembiochem. 2024 Jan 5:e202300870. doi: 10.1002/cbic.202300870. Online ahead of print.ABSTRACTTandem oligonucleotide synthesis (TOS) is an attractive strategy to increase automated oligonucleotide synthesis efficiency. TOS is accomplished via the introduction of an immolative linker within a single sequence composed of multiple oligonucleotide fragments. Here, we report the use of a commercially available building block, typically utilized for the chemical phosphorylation of DNA/RNA oligomers, to perform TOS. We show that the 2,2'-sulfonyldiethylene linker is efficiently self-immolated during the standard deprotection of ...
Source: Chembiochem - January 5, 2024 Category: Biochemistry Authors: Jagandeep S Saraya Derek O'Flaherty Source Type: research
Selection and Characterization of DNA Aptamers for Cytidine and Uridine
This study employs the library-immobilization capture-SELEX technique to isolate aptamers capable of selectively binding to cytidine and uridine. First, a selection was performed using a mixture of cytidine and uridine as the target. This selection led to the isolation of a highly selective aptamer for cytidine with a dissociation constant (Kd ) of 0.9 μM as determined by isothermal titration calorimetry (ITC). In addition, a dual-recognition aptamer was also discovered, which exhibited selective binding to both cytidine and uridine. Subsequently, a separate selection was carried out using uridine as the sole target, and ...
Source: Chembiochem - January 5, 2024 Category: Biochemistry Authors: Lide Gu Jiajie Zheng Yao Zhang Deli Wang Juewen Liu Source Type: research
Solvent-free synthesis of hydrophobic and amphiphilic esters using a chemically modified lipase from Thermomyces lanuginosus: a comparative study with native and immobilized forms
Chembiochem. 2024 Jan 3:e202300843. doi: 10.1002/cbic.202300843. Online ahead of print.ABSTRACTUsing lipases to catalyze the synthesis of the most differentiated type of compounds remains one of the major challenges among scientists. Seeking more economic and advantageous catalysts is a current goal of green chemistry. In this work, we demonstrate the potential of a chemically modified form of lipase from Thermomyces lanuginosus (cmLTL) for the synthesis of both hydrophobic (heptyl heptanoate, heptyl octanoate, heptyl decanoate, decyl heptanoate, decyl octanoate and decyl decanoate) and amphiphilic (2-(2-ethoxyethoxy)ethyl...
Source: Chembiochem - January 3, 2024 Category: Biochemistry Authors: Catarina S Roque Mariana de Castro Tarsila G Castro Carla Silva Artur Cavaco-Paulo Jennifer Noro Source Type: research
Site-Specific Anchoring a Luminescent Tag in a Protein with Non-Emissive Iridium(III) Complex
Chembiochem. 2024 Jan 3:e202300798. doi: 10.1002/cbic.202300798. Online ahead of print.ABSTRACTSite-specific modification of proteins with synthetic fluorescent tag effectively improves the resolution of imaging, and such a labeling method with negligible three-dimensional structural perturbations and minimal impact on the biological functions of proteins is of high interest to dissect the high-resolution activities of biomolecules in complex systems. In this work, several non-emissive iridium(III) complexes [Ir(C^N)2(H2O)2]+OTF- (C^N denotes various cyclometalated ligands) were designed and synthesized. These complexes we...
Source: Chembiochem - January 3, 2024 Category: Biochemistry Authors: Shu-Li Guo Yu-Hao Xiao Bin-Bin Pan Xun-Cheng Su Source Type: research
Solvent-free synthesis of hydrophobic and amphiphilic esters using a chemically modified lipase from Thermomyces lanuginosus: a comparative study with native and immobilized forms
Chembiochem. 2024 Jan 3:e202300843. doi: 10.1002/cbic.202300843. Online ahead of print.ABSTRACTUsing lipases to catalyze the synthesis of the most differentiated type of compounds remains one of the major challenges among scientists. Seeking more economic and advantageous catalysts is a current goal of green chemistry. In this work, we demonstrate the potential of a chemically modified form of lipase from Thermomyces lanuginosus (cmLTL) for the synthesis of both hydrophobic (heptyl heptanoate, heptyl octanoate, heptyl decanoate, decyl heptanoate, decyl octanoate and decyl decanoate) and amphiphilic (2-(2-ethoxyethoxy)ethyl...
Source: Chembiochem - January 3, 2024 Category: Biochemistry Authors: Catarina S Roque Mariana de Castro Tarsila G Castro Carla Silva Artur Cavaco-Paulo Jennifer Noro Source Type: research
