Synthesis of new bodipy hydrazide fluorescent probes for the detection of carbonylated proteins generated by oxidative stress

We describe the design and the synthetic pathway of new BODIPY fluorescent probes functionalized with hydrazide function for protein carbonyl labeling to improve existing methodologies such as 2D-Oxi electrophoresis. Hydrazide BODIPY analogues show very good fluorescent properties such as NIR emission up to 633 nm and quantum yield up to 0.88. These new probes were validated for the detection and quantification of carbonylated proteins with 2D-Oxi electrophoresis using mouse muscle protein extracts, as well as both flow cytometry and microscopy using oxidant stressed C2C12 cells.PMID:38695553 | DOI:10.1002/cbic.202400093

Source: Chembiochem - May 2, 2024 Category: Biochemistry Authors: Anthony Nina Diogo Janek Hyzewicz Marie-Paule Hamon Jeremy Fort é Serge Thorimbert Bertrand Friguet Candice Botuha Source Type: research