PSL Chemical Biology Symposia: Recent Progress in Ferroptosis
Chembiochem. 2024 Mar 26:e202400211. doi: 10.1002/cbic.202400211. Online ahead of print.ABSTRACTThis symposium is the 5th PSL (Paris Sciences & Lettres) Chemical Biology meeting (2015, 2016, 2019, 2023, 2024) held at Institut Curie. This initiative originally started at Institut de Chimie des Substances Naturelles (ICSN) in Gif-sur-Yvette, with a strong focus on chemistry. It was then continued at the Institut Curie (2015) covering a larger scope, before becoming the official PSL Chemical Biology meeting. This latest edition hosted around 150 participants and was focused on the burgeoning field of ferroptosis, its mech...
Source: Chembiochem - March 26, 2024 Category: Biochemistry Authors: Arthur Pereira Leeroy Baron Romain Bucci Marina Plays Giulia Bonasegale Armel Picard-Bernes Manuel Bibrowski Nolwenn Morris Sacha Marynberg Fabien Sindikubwabo Tatiana Ca ñeque Sebastian M üller Ludovic Colombeau St éphanie Solier Yannick Bono Christin Source Type: research
Cysteine-independent CRISPR-associated protein labeling for presentation and co-delivery of molecules toward genetic and epigenetic regulations
We report a method that does not require cysteine residues for small molecule presentation on the CRISPR-associated protein SpCas9 for in vitro protein detection, probing cellular protein expression, and nuclear co-delivery of molecules in mammalian cells. We repurposed a simple protein purification tag His6 peptide for non-covalent labeling of molecules on the CRISPR enzyme SpCas9. The small molecule labeling enabled us to detect SpCas9 in a biochemical assay. We demonstrate that small molecule labeling can be utilized for probing bacterial protein expression in realtime. Furthermore, we coupled SpCas9's nuclear-targeting...
Source: Chembiochem - March 26, 2024 Category: Biochemistry Authors: Sadiya Tanga Arpita Hota Arkadeep Karmakar Paramita Banerjee Basudeb Maji Source Type: research
Structure-Based Site-Directed Mutagenesis of Hydroxynitrile Lyase from Cyanogenic Millipede, Oxidus gracilis for hydrocyanation and Henry reactions
Chembiochem. 2024 Mar 25:e202400118. doi: 10.1002/cbic.202400118. Online ahead of print.ABSTRACTHydroxynitrile lyase (HNL) from cyanogenic millipede Oxidus gracillis (OgraHNL) has been discovered as a crucial enzyme involving in the cyanogenesis pathway. Here, the crystal structures of OgraHNL complexed with sulfate, benzaldehyde (BA), (R)-Man, (R)-2-Cl-Man, and acetone cyanohydrin (ACN) were solved at 1.6, 1.7, 2.3, 2.1, and 2.0 Å resolutions, respectively. The structure of OgraHNL revealed that it belongs to the lipocalin superfamily. Based on the structure, positive mutants have been designed to further improve the cat...
Source: Chembiochem - March 25, 2024 Category: Biochemistry Authors: Siriporn Chaikaew Yukio Watanabe Daijun Zheng Fumihiro Motojima Takuya Yamaguchi Yasuhisa Asano Source Type: research
Structure-Based Site-Directed Mutagenesis of Hydroxynitrile Lyase from Cyanogenic Millipede, Oxidus gracilis for hydrocyanation and Henry reactions
Chembiochem. 2024 Mar 25:e202400118. doi: 10.1002/cbic.202400118. Online ahead of print.ABSTRACTHydroxynitrile lyase (HNL) from cyanogenic millipede Oxidus gracillis (OgraHNL) has been discovered as a crucial enzyme involving in the cyanogenesis pathway. Here, the crystal structures of OgraHNL complexed with sulfate, benzaldehyde (BA), (R)-Man, (R)-2-Cl-Man, and acetone cyanohydrin (ACN) were solved at 1.6, 1.7, 2.3, 2.1, and 2.0 Å resolutions, respectively. The structure of OgraHNL revealed that it belongs to the lipocalin superfamily. Based on the structure, positive mutants have been designed to further improve the cat...
Source: Chembiochem - March 25, 2024 Category: Biochemistry Authors: Siriporn Chaikaew Yukio Watanabe Daijun Zheng Fumihiro Motojima Takuya Yamaguchi Yasuhisa Asano Source Type: research
Climbing into their Skin to Understand Contextual Protein-Protein Associations and Localizations: Functional Investigations in Transgenic Live Model Organisms
Chembiochem. 2024 Mar 21:e202400005. doi: 10.1002/cbic.202400005. Online ahead of print.ABSTRACTBorrowing some quotes from Harper Lee's novel "To Kill A Mockingbird" to help frame our manuscript, we discuss methods to profile local proteomes. We initially focus on chemical biology regimens that function in live organisms and use reactive biotin species for this purpose. We then consider ways to add new dimensions to these experimental regimens, principally by releasing less reactive (i. e., more selective) (preter)natural electrophiles. Although electrophile release methods may have lower resolution and label fewer protein...
Source: Chembiochem - March 21, 2024 Category: Biochemistry Authors: Marcus J C Long Yimon Aye Source Type: research
Climbing into their Skin to Understand Contextual Protein-Protein Associations and Localizations: Functional Investigations in Transgenic Live Model Organisms
Chembiochem. 2024 Mar 21:e202400005. doi: 10.1002/cbic.202400005. Online ahead of print.ABSTRACTBorrowing some quotes from Harper Lee's novel "To Kill A Mockingbird" to help frame our manuscript, we discuss methods to profile local proteomes. We initially focus on chemical biology regimens that function in live organisms and use reactive biotin species for this purpose. We then consider ways to add new dimensions to these experimental regimens, principally by releasing less reactive (i. e., more selective) (preter)natural electrophiles. Although electrophile release methods may have lower resolution and label fewer protein...
Source: Chembiochem - March 21, 2024 Category: Biochemistry Authors: Marcus J C Long Yimon Aye Source Type: research
Climbing into their Skin to Understand Contextual Protein-Protein Associations and Localizations: Functional Investigations in Transgenic Live Model Organisms
Chembiochem. 2024 Mar 21:e202400005. doi: 10.1002/cbic.202400005. Online ahead of print.ABSTRACTBorrowing some quotes from Harper Lee's novel "To Kill A Mockingbird" to help frame our manuscript, we discuss methods to profile local proteomes. We initially focus on chemical biology regimens that function in live organisms and use reactive biotin species for this purpose. We then consider ways to add new dimensions to these experimental regimens, principally by releasing less reactive (i. e., more selective) (preter)natural electrophiles. Although electrophile release methods may have lower resolution and label fewer protein...
Source: Chembiochem - March 21, 2024 Category: Biochemistry Authors: Marcus J C Long Yimon Aye Source Type: research
Engineering the activity of a newly identified arylalkylamine N-acetyltransferase in the acetylation of 5-hydroxytryptamine
This study broadens the repertoire of AANAT for the efficient biosynthesis of melatonin.PMID:38504591 | DOI:10.1002/cbic.202400069 (Source: Chembiochem)
Source: Chembiochem - March 20, 2024 Category: Biochemistry Authors: Qing Wang Congcong Li Bo Yuan Aiqun Yu Ge Qu Zhoutong Sun Source Type: research
Stereoselective synthesis of the Gal- α-(1→3)-Gal-β-(1→3)-GlcNAc trisaccharide: a new ligand for DCAR and Mincle C-type lectin receptors
Chembiochem. 2024 Mar 20:e202400026. doi: 10.1002/cbic.202400026. Online ahead of print.ABSTRACTIn this work, we have discovered that the Gal-α-(1→3)-Gal-β-(1→3)-GlcNAc trisaccharide, a fragment of the B antigen Type-1, is a new ligand of two C-type lectin receptors (CLRs) i.e. DCAR and Mincle which are key players in different types of autoimmune diseases. Accordingly, we report here on a straightforward methodology to access pure Gal-α-(1→3)-Gal-β-(1→3)-GlcNAc trisaccharide. A spacer with a terminal primary amine group was included to the reducing end of the GlcNAc residue thus ensuring the further functional...
Source: Chembiochem - March 20, 2024 Category: Biochemistry Authors: Jacopo Tricomi Mike Aoun Bingze Xu Rikard Holmdahl Barbara Richichi Source Type: research
Engineering the activity of a newly identified arylalkylamine N-acetyltransferase in the acetylation of 5-hydroxytryptamine
This study broadens the repertoire of AANAT for the efficient biosynthesis of melatonin.PMID:38504591 | DOI:10.1002/cbic.202400069 (Source: Chembiochem)
Source: Chembiochem - March 20, 2024 Category: Biochemistry Authors: Qing Wang Congcong Li Bo Yuan Aiqun Yu Ge Qu Zhoutong Sun Source Type: research
Stereoselective synthesis of the Gal- α-(1→3)-Gal-β-(1→3)-GlcNAc trisaccharide: a new ligand for DCAR and Mincle C-type lectin receptors
Chembiochem. 2024 Mar 20:e202400026. doi: 10.1002/cbic.202400026. Online ahead of print.ABSTRACTIn this work, we have discovered that the Gal-α-(1→3)-Gal-β-(1→3)-GlcNAc trisaccharide, a fragment of the B antigen Type-1, is a new ligand of two C-type lectin receptors (CLRs) i.e. DCAR and Mincle which are key players in different types of autoimmune diseases. Accordingly, we report here on a straightforward methodology to access pure Gal-α-(1→3)-Gal-β-(1→3)-GlcNAc trisaccharide. A spacer with a terminal primary amine group was included to the reducing end of the GlcNAc residue thus ensuring the further functional...
Source: Chembiochem - March 20, 2024 Category: Biochemistry Authors: Jacopo Tricomi Mike Aoun Bingze Xu Rikard Holmdahl Barbara Richichi Source Type: research
Synthesis of Pharmaceutically Relevant Arylamines Enabled by a Nitroreductase from Bacillus tequilensis
In this study, we assessed a novel NR from Bacillus tequilensis, named BtNR, for the synthesis of pharmaceutically relevant arylamines, including valuable synthons used in the manufacture of blockbuster drugs such as vismodegib, sonidegib, linezolid and sildenafil. After optimizing the enzymatic reaction conditions, high conversion of nitroaromatics to arylamines (up to 97%) and good product yields (up to 56%) were achieved. Our results indicate that BtNR has a broad substrate scope, including bulky nitro benzenes, nitro pyrazoles and nitro pyridines. Hence, BtNR is an interesting biocatalyst for the synthesis of pharmaceu...
Source: Chembiochem - March 19, 2024 Category: Biochemistry Authors: Sara Russo Alejandro Prats Luj án Marco Fraaije Gerrit J Poelarends Source Type: research
Synthesis of Pharmaceutically Relevant Arylamines Enabled by a Nitroreductase from Bacillus tequilensis
In this study, we assessed a novel NR from Bacillus tequilensis, named BtNR, for the synthesis of pharmaceutically relevant arylamines, including valuable synthons used in the manufacture of blockbuster drugs such as vismodegib, sonidegib, linezolid and sildenafil. After optimizing the enzymatic reaction conditions, high conversion of nitroaromatics to arylamines (up to 97%) and good product yields (up to 56%) were achieved. Our results indicate that BtNR has a broad substrate scope, including bulky nitro benzenes, nitro pyrazoles and nitro pyridines. Hence, BtNR is an interesting biocatalyst for the synthesis of pharmaceu...
Source: Chembiochem - March 19, 2024 Category: Biochemistry Authors: Sara Russo Alejandro Prats Luj án Marco Fraaije Gerrit J Poelarends Source Type: research
Natural Products That Contain Higher Homologated Amino Acids
Chembiochem. 2024 Mar 15:e202300822. doi: 10.1002/cbic.202300822. Online ahead of print.ABSTRACTThis review focuses on discussing natural products (NPs) that contain higher homologs of amino acids (homoAAs) in the structure as well as the proposed and characterized biosynthesis of these non-proteinogenic amino acids. Homologation of amino acids includes the insertion of a methylene group into its side chain. It is not a very common modification found in NP biosynthesis as approximately 450 homoAA-containing NPs have been isolated from four bacterial phyla (Cyanobacteria, Actinomycetota, Myxococcota, and Pseudomonadota), tw...
Source: Chembiochem - March 15, 2024 Category: Biochemistry Authors: Skyler L Owens Shopno R Ahmed Rebecca M Lang Laura E Stewart Shogo Mori Source Type: research
Dual-Emissive Iridium(III) Complexes and Their Applications in Biological Sensing and Imaging
Chembiochem. 2024 Mar 15:e202400094. doi: 10.1002/cbic.202400094. Online ahead of print.ABSTRACTPhosphorescent iridium(III) complexes are widely recognized for their unique properties in the excited triplet state, making them crucial for various applications including biological sensing and imaging. Most of these complexes display single phosphorescence emission from the lowest-lying triplet state after undergoing highly efficient intersystem crossing (ISC) and ultrafast internal conversion (IC) processes. However, in cases where these excited-state processes are restricted, the less common phenomenon of dual emission has ...
Source: Chembiochem - March 15, 2024 Category: Biochemistry Authors: Zhipeng Ruan Jun Yang Yonghua Li Kenneth Yin Zhang Source Type: research
