Natural Products That Contain Higher Homologated Amino Acids
Chembiochem. 2024 Mar 15:e202300822. doi: 10.1002/cbic.202300822. Online ahead of print.ABSTRACTThis review focuses on discussing natural products (NPs) that contain higher homologs of amino acids (homoAAs) in the structure as well as the proposed and characterized biosynthesis of these non-proteinogenic amino acids. Homologation of amino acids includes the insertion of a methylene group into its side chain. It is not a very common modification found in NP biosynthesis as approximately 450 homoAA-containing NPs have been isolated from four bacterial phyla (Cyanobacteria, Actinomycetota, Myxococcota, and Pseudomonadota), tw...
Source: Chembiochem - March 15, 2024 Category: Biochemistry Authors: Skyler L Owens Shopno R Ahmed Rebecca M Lang Laura E Stewart Shogo Mori Source Type: research
Dual-Emissive Iridium(III) Complexes and Their Applications in Biological Sensing and Imaging
Chembiochem. 2024 Mar 15:e202400094. doi: 10.1002/cbic.202400094. Online ahead of print.ABSTRACTPhosphorescent iridium(III) complexes are widely recognized for their unique properties in the excited triplet state, making them crucial for various applications including biological sensing and imaging. Most of these complexes display single phosphorescence emission from the lowest-lying triplet state after undergoing highly efficient intersystem crossing (ISC) and ultrafast internal conversion (IC) processes. However, in cases where these excited-state processes are restricted, the less common phenomenon of dual emission has ...
Source: Chembiochem - March 15, 2024 Category: Biochemistry Authors: Zhipeng Ruan Jun Yang Yonghua Li Kenneth Yin Zhang Source Type: research
Natural Products That Contain Higher Homologated Amino Acids
Chembiochem. 2024 Mar 15:e202300822. doi: 10.1002/cbic.202300822. Online ahead of print.ABSTRACTThis review focuses on discussing natural products (NPs) that contain higher homologs of amino acids (homoAAs) in the structure as well as the proposed and characterized biosynthesis of these non-proteinogenic amino acids. Homologation of amino acids includes the insertion of a methylene group into its side chain. It is not a very common modification found in NP biosynthesis as approximately 450 homoAA-containing NPs have been isolated from four bacterial phyla (Cyanobacteria, Actinomycetota, Myxococcota, and Pseudomonadota), tw...
Source: Chembiochem - March 15, 2024 Category: Biochemistry Authors: Skyler L Owens Shopno R Ahmed Rebecca M Lang Laura E Stewart Shogo Mori Source Type: research
Dual-Emissive Iridium(III) Complexes and Their Applications in Biological Sensing and Imaging
Chembiochem. 2024 Mar 15:e202400094. doi: 10.1002/cbic.202400094. Online ahead of print.ABSTRACTPhosphorescent iridium(III) complexes are widely recognized for their unique properties in the excited triplet state, making them crucial for various applications including biological sensing and imaging. Most of these complexes display single phosphorescence emission from the lowest-lying triplet state after undergoing highly efficient intersystem crossing (ISC) and ultrafast internal conversion (IC) processes. However, in cases where these excited-state processes are restricted, the less common phenomenon of dual emission has ...
Source: Chembiochem - March 15, 2024 Category: Biochemistry Authors: Zhipeng Ruan Jun Yang Yonghua Li Kenneth Yin Zhang Source Type: research
Natural Products That Contain Higher Homologated Amino Acids
Chembiochem. 2024 Mar 15:e202300822. doi: 10.1002/cbic.202300822. Online ahead of print.ABSTRACTThis review focuses on discussing natural products (NPs) that contain higher homologs of amino acids (homoAAs) in the structure as well as the proposed and characterized biosynthesis of these non-proteinogenic amino acids. Homologation of amino acids includes the insertion of a methylene group into its side chain. It is not a very common modification found in NP biosynthesis as approximately 450 homoAA-containing NPs have been isolated from four bacterial phyla (Cyanobacteria, Actinomycetota, Myxococcota, and Pseudomonadota), tw...
Source: Chembiochem - March 15, 2024 Category: Biochemistry Authors: Skyler L Owens Shopno R Ahmed Rebecca M Lang Laura E Stewart Shogo Mori Source Type: research
Dual-Emissive Iridium(III) Complexes and Their Applications in Biological Sensing and Imaging
Chembiochem. 2024 Mar 15:e202400094. doi: 10.1002/cbic.202400094. Online ahead of print.ABSTRACTPhosphorescent iridium(III) complexes are widely recognized for their unique properties in the excited triplet state, making them crucial for various applications including biological sensing and imaging. Most of these complexes display single phosphorescence emission from the lowest-lying triplet state after undergoing highly efficient intersystem crossing (ISC) and ultrafast internal conversion (IC) processes. However, in cases where these excited-state processes are restricted, the less common phenomenon of dual emission has ...
Source: Chembiochem - March 15, 2024 Category: Biochemistry Authors: Zhipeng Ruan Jun Yang Yonghua Li Kenneth Yin Zhang Source Type: research
Molecular Driving Forces in the Self-Association of Silaffin Peptide R5 from MD Simulations
Chembiochem. 2024 Mar 14:e202300788. doi: 10.1002/cbic.202300788. Online ahead of print.ABSTRACTThe 19-residue silaffin-R5 peptide has been widely studied for its ability to precipitate uniform SiO2 particles through mild temperature and pH pathways, in the absence of any organic solvents. There is consensus that post-translational modification (PTM) of side chains has a large impact on the biomineralization process. Thus, it is imperative to understand the precise mechanisms that dictate the formation of SiO2 from R5 peptide, including the effects of PTM on peptide aggregation and peptide-surface adsorption. In this work,...
Source: Chembiochem - March 14, 2024 Category: Biochemistry Authors: Coco Moke Mao Janani Sampath Jim Pfaendtner Source Type: research
Monitoring Anthracycline Cancer Drug-Nucleosome Interaction by NMR using a Specific Isotope Labeling Approach for Nucleosomal DNA
We describe the synthesis of segmental one-strand 13C-thymidine labeled 601-DNA, the assignment of the methyl signals, and demonstrate its use to observe site-specific binding events in the nucleosome by aclarubicin, an anthracycline cancer drug that intercalates into the DNA minor grooves. Our results highlight intrinsic conformational heterogeneity in the 601 DNA sequence and show that aclarubicin binds an exposed AT-rich region near the DNA end. Overall, our data point to a model where the drug invades the nucleosome from the terminal ends inward, eventually resulting in histone eviction and nucleosome disruption.PMID:3...
Source: Chembiochem - March 13, 2024 Category: Biochemistry Authors: Clara L van Emmerik Vincenzo Lobbia Jacques Neefjes Frank H T Nelissen Hugo van Ingen Source Type: research
Novel Triazole-Containing "Dipeptides": Synthesis, Molecular Docking And Analgesic Activity Studies
Chembiochem. 2024 Mar 13:e202300837. doi: 10.1002/cbic.202300837. Online ahead of print.ABSTRACTDipeptides of a new structure based on β-triazolalanines and (L)-α-amino acids were synthesized and optimal conditions were developed that ensure both chemical and optical purity of the final products. Molecular docking was carried out and possible intermolecular interactions of dipeptides with potential targets were established. Based on these studies, the analgesic property of chosen dipeptides was studied and it was found that some compounds possess revealed antinociceptive activity in the tail-flick test.PMID:38477021 | DO...
Source: Chembiochem - March 13, 2024 Category: Biochemistry Authors: Tariel V Ghochikyan Arusyak G Zhamharyan Shushanik G Afrikyan Vardges R Frangyan Armen Galstyan Source Type: research
Research Progress on the Assembly of Large DNA Fragments
This article provides an overview of the development and current state of DNA assembly technology, with a focus on recent advancements in the assembly of large DNA fragments in Escherichia coli, Bacillus subtilis, and Saccharomyces cerevisiae. In particular, the methods and challenges associated with the assembly of large DNA fragment in different hosts are highlighted. The advancements in DNA assembly have the potential to facilitate the construction of customized genomes, giving us the ability to modify cellular functions and even create artificial life. It is also contributing to our ability to understand, predict, and ...
Source: Chembiochem - March 13, 2024 Category: Biochemistry Authors: Wei Tan Qing Miao Xuemei Jia Ying Liu Shuai Li Dayong Yang Source Type: research
Efficient Transferase Engineering for SAM Analog Synthesis from Iodoalkanes
Chembiochem. 2024 Mar 13:e202400079. doi: 10.1002/cbic.202400079. Online ahead of print.ABSTRACTS-Adenosyl-l-methionine (SAM) is an important cosubstrate in various biochemical processes, including selective methyl transfer reactions. Simple methods for the (re)generation of SAM analogs could expand the chemistry accessible with SAM-dependent transferases and go beyond methylation chemistry. Here we present an efficient enzyme engineering strategy to synthesize different SAM analogs from "off-the-shelf" iodoalkanes through enzymatic alkylation of S-adenosyl-l-homocysteine (SAH). This was achieved by mutating multiple hydro...
Source: Chembiochem - March 13, 2024 Category: Biochemistry Authors: Kai H Sch ülke Jana S Fr öse Alina Klein Marc Garcia-Borr às Stephan C Hammer Source Type: research
Porphyrin-BODIPY Dyad: Enhancing Photodynamic Inactivation via Antenna Effect
Chembiochem. 2024 Mar 13:e202400138. doi: 10.1002/cbic.202400138. Online ahead of print.ABSTRACTA porphyrin-BODIPY dyad (P-BDP) was obtained through covalent bonding, featuring a two-segment design comprising a light-harvesting antenna system connected to an energy acceptor unit. The absorption spectrum of P-BDP resulted from an overlap of the individual spectra of its constituent parts, with the fluorescence emission of the BODIPY unit experiencing significant quenching (96%) due to the presence of the porphyrin unit. Spectroscopic, computational, and redox investigations revealed a competition between photoinduced energy...
Source: Chembiochem - March 13, 2024 Category: Biochemistry Authors: Mar ía Eugenia Pérez Javier Esteban Durantini Sol Romina Martinez Andr és Matías Durantini Mar ía Elisa Milanesio Edgardo N Durantini Source Type: research
Monitoring Anthracycline Cancer Drug-Nucleosome Interaction by NMR using a Specific Isotope Labeling Approach for Nucleosomal DNA
We describe the synthesis of segmental one-strand 13C-thymidine labeled 601-DNA, the assignment of the methyl signals, and demonstrate its use to observe site-specific binding events in the nucleosome by aclarubicin, an anthracycline cancer drug that intercalates into the DNA minor grooves. Our results highlight intrinsic conformational heterogeneity in the 601 DNA sequence and show that aclarubicin binds an exposed AT-rich region near the DNA end. Overall, our data point to a model where the drug invades the nucleosome from the terminal ends inward, eventually resulting in histone eviction and nucleosome disruption.PMID:3...
Source: Chembiochem - March 13, 2024 Category: Biochemistry Authors: Clara L van Emmerik Vincenzo Lobbia Jacques Neefjes Frank H T Nelissen Hugo van Ingen Source Type: research
Novel Triazole-Containing "Dipeptides": Synthesis, Molecular Docking And Analgesic Activity Studies
Chembiochem. 2024 Mar 13:e202300837. doi: 10.1002/cbic.202300837. Online ahead of print.ABSTRACTDipeptides of a new structure based on β-triazolalanines and (L)-α-amino acids were synthesized and optimal conditions were developed that ensure both chemical and optical purity of the final products. Molecular docking was carried out and possible intermolecular interactions of dipeptides with potential targets were established. Based on these studies, the analgesic property of chosen dipeptides was studied and it was found that some compounds possess revealed antinociceptive activity in the tail-flick test.PMID:38477021 | DO...
Source: Chembiochem - March 13, 2024 Category: Biochemistry Authors: Tariel V Ghochikyan Arusyak G Zhamharyan Shushanik G Afrikyan Vardges R Frangyan Armen Galstyan Source Type: research
Research Progress on the Assembly of Large DNA Fragments
This article provides an overview of the development and current state of DNA assembly technology, with a focus on recent advancements in the assembly of large DNA fragments in Escherichia coli, Bacillus subtilis, and Saccharomyces cerevisiae. In particular, the methods and challenges associated with the assembly of large DNA fragment in different hosts are highlighted. The advancements in DNA assembly have the potential to facilitate the construction of customized genomes, giving us the ability to modify cellular functions and even create artificial life. It is also contributing to our ability to understand, predict, and ...
Source: Chembiochem - March 13, 2024 Category: Biochemistry Authors: Wei Tan Qing Miao Xuemei Jia Ying Liu Shuai Li Dayong Yang Source Type: research
