Single-cell RNA sequencing technology in human spermatogenesis: Progresses and perspectives
Mol Cell Biochem. 2023 Sep 2. doi: 10.1007/s11010-023-04840-x. Online ahead of print.ABSTRACTSpermatogenesis, a key part of the spermiation process, is regulated by a combination of key cells, such as primordial germ cells, spermatogonial stem cells, and somatic cells, such as Sertoli cells. Abnormal spermatogenesis can lead to azoospermia, testicular tumors, and other diseases related to male infertility. The application of single-cell RNA sequencing (scRNA-seq) technology in male reproduction is gradually increasing with its unique insight into deep mining and analysis. The data cover different periods of neonatal, prepu...
Source: Molecular and Cellular Biochemistry - September 2, 2023 Category: Biochemistry Authors: Hanbo Jia Wei Wang Zhaowen Zhou Zhiyi Chen Zijun Lan Hao Bo Liqing Fan Source Type: research
Single-cell RNA sequencing technology in human spermatogenesis: Progresses and perspectives
Mol Cell Biochem. 2023 Sep 2. doi: 10.1007/s11010-023-04840-x. Online ahead of print.ABSTRACTSpermatogenesis, a key part of the spermiation process, is regulated by a combination of key cells, such as primordial germ cells, spermatogonial stem cells, and somatic cells, such as Sertoli cells. Abnormal spermatogenesis can lead to azoospermia, testicular tumors, and other diseases related to male infertility. The application of single-cell RNA sequencing (scRNA-seq) technology in male reproduction is gradually increasing with its unique insight into deep mining and analysis. The data cover different periods of neonatal, prepu...
Source: Molecular and Cellular Biochemistry - September 2, 2023 Category: Biochemistry Authors: Hanbo Jia Wei Wang Zhaowen Zhou Zhiyi Chen Zijun Lan Hao Bo Liqing Fan Source Type: research
Single-cell RNA sequencing technology in human spermatogenesis: Progresses and perspectives
Mol Cell Biochem. 2023 Sep 2. doi: 10.1007/s11010-023-04840-x. Online ahead of print.ABSTRACTSpermatogenesis, a key part of the spermiation process, is regulated by a combination of key cells, such as primordial germ cells, spermatogonial stem cells, and somatic cells, such as Sertoli cells. Abnormal spermatogenesis can lead to azoospermia, testicular tumors, and other diseases related to male infertility. The application of single-cell RNA sequencing (scRNA-seq) technology in male reproduction is gradually increasing with its unique insight into deep mining and analysis. The data cover different periods of neonatal, prepu...
Source: Molecular and Cellular Biochemistry - September 2, 2023 Category: Biochemistry Authors: Hanbo Jia Wei Wang Zhaowen Zhou Zhiyi Chen Zijun Lan Hao Bo Liqing Fan Source Type: research
First report of primary testicular leiomyosarcoma in two dogs
CONCLUSIONS: To the authors knowledge these are the first cases of primary testicular sarcoma reported in the canine species. However, even rare, these tumours deserve to be considered in routine diagnosis when a testicular spindle cell tumour is observed. The immunohistochemical panel applied was useful to distinguish the present tumours from undifferentiated Sertoli cell tumours confirming the diagnosis of leiomyosarcoma.PMID:37525233 | DOI:10.1186/s12917-023-03658-5 (Source: Cell Research)
Source: Cell Research - July 31, 2023 Category: Cytology Authors: Rafa ł Ciaputa Eleonora Brambilla Francesco Godizzi Stanis ław Dzimira Kacper Żebrowski Ma łgorzata Kandefer-Gola Marcin Nowak Valeria Grieco Source Type: research
Gastrodin protects porcine sertoli cells from zearalenone-induced abnormal secretion of glial cell line-derived neurotrophic factor through the NOTCH signaling pathway
In conclusion, the present study demonstrates that ZEA disrupts pSSCs self-renewal by affecting the function of porcine Sertoli cell, and highlights the protective mechanism of GAS through the regulation of the NOTCH signaling pathway. These findings may offer a novel strategy for alleviating ZEA-induced male reproductive dysfunction in animal production.PMID:37285694 | DOI:10.1016/j.repbio.2023.100781 (Source: Reproductive Biology)
Source: Reproductive Biology - June 7, 2023 Category: Reproduction Medicine Authors: Yulu Cao Jingjing Wang Xiaoxiao Li Biyun Liu Chongjun Li Yijin Sun Kang Zou Source Type: research
Gastrodin protects porcine sertoli cells from zearalenone-induced abnormal secretion of glial cell line-derived neurotrophic factor through the NOTCH signaling pathway
In conclusion, the present study demonstrates that ZEA disrupts pSSCs self-renewal by affecting the function of porcine Sertoli cell, and highlights the protective mechanism of GAS through the regulation of the NOTCH signaling pathway. These findings may offer a novel strategy for alleviating ZEA-induced male reproductive dysfunction in animal production.PMID:37285694 | DOI:10.1016/j.repbio.2023.100781 (Source: Reproductive Biology)
Source: Reproductive Biology - June 7, 2023 Category: Reproduction Medicine Authors: Yulu Cao Jingjing Wang Xiaoxiao Li Biyun Liu Chongjun Li Yijin Sun Kang Zou Source Type: research
The sclerosing sertoli cell tumor of the testis: a case report
Testicular Sertoli cell tumor (SCT) is very rare sex cord-gonadal stromal tumor, and sclerosing SCT (SSCT) is even rarer. So far, no more than 50 cases of SSCT have been reported. 80% of SSCTs are less than 2 ... (Source: Diagnostic Pathology)
Source: Diagnostic Pathology - May 15, 2023 Category: Pathology Authors: Xueyao Tang, Yifan Hu, Hong Zhou and Yang Zhou Tags: Case Report Source Type: research
A single-nucleus transcriptomic atlas of primate testicular aging reveals exhaustion of the spermatogonial stem cell reservoir and loss of Sertoli cell homeostasis
Protein Cell. 2022 Nov 18:pwac057. doi: 10.1093/procel/pwac057. Online ahead of print.ABSTRACTThe testis is pivotal for male reproduction, and its progressive functional decline in aging is associated with infertility. However, the regulatory mechanism underlying primate testicular aging remains largely elusive. Here, we resolve the aging-related cellular and molecular alterations of primate testicular aging by establishing a single-nucleus transcriptomic atlas. Gene-expression patterns along the spermatogenesis trajectory revealed molecular programs associated with attrition of spermatogonial stem cell reservoir, disturbe...
Source: Protein and Cell - March 17, 2023 Category: Cytology Authors: Daoyuan Huang Yuesheng Zuo Chen Zhang Guoqiang Sun Ying Jing Jinghui Lei Shuai Ma Shuhui Sun Huifen Lu Yusheng Cai Weiqi Zhang Fei Gao Andy Peng Xiang Juan Carlos Izpisua Belmonte Guang-Hui Liu Jing Qu Si Wang Source Type: research
Testicular impairment in Primary Adrenal Insufficiency caused by Nicotinamide Nucleotide Transhydrogenase (NNT) deficiency - a case report: implication of oxidative stress and importance of fertility preservation
ConclusionThis case report provides, for the first time, direct evidence of complete germ line loss in an azoospermic man with NNT deficiency. Additional data further support the hypothesis of a determinant role of oxidative cellular damage due to reactive oxygen species (ROS) imbalance in the severe gonadal impairment observed in this NNT-deficient patient. Early and regular evaluation of gonadal function should be performed in patients with PAI, especially with NNT deficiency, as soon as the patients reach puberty. Fertility preservation options should then be provided in early adulthood for these patients. (Source: Basi...
Source: Basic and Clinical Andrology - March 14, 2023 Category: Urology & Nephrology Source Type: research
Analysis of connexin 43, connexin 45 and N-cadherin in the human sertoli cell line FS1 and the human seminoma-like cell line TCam-2 in comparison with human testicular biopsies
Germ cell tumors are relatively common in young men. They derive from a non-invasive precursor, called germ cell neoplasia in situ, but the exact pathogenesis is still unknown. Thus, further understanding prov... (Source: BMC Cancer)
Source: BMC Cancer - March 10, 2023 Category: Cancer & Oncology Authors: Birte Schulz, Val érie Schumacher, Anaclet Ngezahayo, Daniela Maier-Begandt, Nadine Schadzek, Jochen Wilhelm, Wolfgang Weidner, Adrian Pilatz, Daniela Fietz, Sabine Kliesch, Nadine Schnepel, Nina Hambruch, Kristina Rode, Marion Langeheine and Ralph Brehm Tags: Research Source Type: research
Utilization of NKX3.1, P501S, Prostate-Specific Antigen, and Steroidogenic Factor 1 to Distinguish Malignant Leydig Cell Tumor From Metastatic Prostatic Adenocarcinoma to the Testis
CONCLUSIONS.—: The diagnosis of malignant Leydig cell tumor and its distinction from metastatic adenocarcinoma to the testis can be made immunohistochemically on the basis of SF-1 positivity and negativity for NKX3.1.PMID:36881770 | DOI:10.5858/arpa.2022-0424-OA (Source: Archives of Pathology and Laboratory Medicine)
Source: Archives of Pathology and Laboratory Medicine - March 7, 2023 Category: Laboratory Medicine Authors: Eric Erak Thomas M Ulbright Jonathan Epstein Source Type: research
Utilization of NKX3.1, P501S, Prostate-Specific Antigen, and Steroidogenic Factor 1 to Distinguish Malignant Leydig Cell Tumor From Metastatic Prostatic Adenocarcinoma to the Testis
CONCLUSIONS.—: The diagnosis of malignant Leydig cell tumor and its distinction from metastatic adenocarcinoma to the testis can be made immunohistochemically on the basis of SF-1 positivity and negativity for NKX3.1.PMID:36881770 | DOI:10.5858/arpa.2022-0424-OA (Source: Archives of Pathology and Laboratory Medicine)
Source: Archives of Pathology and Laboratory Medicine - March 7, 2023 Category: Laboratory Medicine Authors: Eric Erak Thomas M Ulbright Jonathan Epstein Source Type: research
Utilization of NKX3.1, P501S, Prostate-Specific Antigen, and Steroidogenic Factor 1 to Distinguish Malignant Leydig Cell Tumor From Metastatic Prostatic Adenocarcinoma to the Testis
CONCLUSIONS.—: The diagnosis of malignant Leydig cell tumor and its distinction from metastatic adenocarcinoma to the testis can be made immunohistochemically on the basis of SF-1 positivity and negativity for NKX3.1.PMID:36881770 | DOI:10.5858/arpa.2022-0424-OA (Source: Archives of Pathology and Laboratory Medicine)
Source: Archives of Pathology and Laboratory Medicine - March 7, 2023 Category: Laboratory Medicine Authors: Eric Erak Thomas M Ulbright Jonathan Epstein Source Type: research
Streptococcus canis prostatitis and endocarditis with thromboembolism in a dog with sertoli cell tumour in a cryptic testis and prostatic squamous metaplasia
We describe an unusual case of prostatitis caused byStreptococcus canis evolving to endocarditis and splenic, renal, and cerebral thromboembolism in a dog, associated with a Sertoli cell tumour in a cryptic testis and diffuse prostatic squamous metaplasia. A nine-year-old, intact male, mixed-breed dog was presented to a veterinary teaching hospital with abdominal pain and prostration. Physical examination and abdominal ultrasonography revealed an atrophic right testicle located in the subcutaneous tissue. The left testicle was in the abdominal cavity with increased dimensions and irregular contours. Complete blood count an...
Source: Veterinary Research Communications - January 31, 2023 Category: Veterinary Research Source Type: research
