Effect of androgens on Sertoli cell maturation in human testis from birth to puberty
ConclusionsAndrogen action on Sertoli cells is required for onset of spermatogenesis and premature androgen secretion by LCT can induce spermatogenesis in the vicinity of the tumor. AR ir appeared earlier than onset of spermatogenesis, with large interindividual variability. The timing and mechanisms of Sertoli cell responsiveness to androgens are important issues for understanding the induction of spermatogenesis at puberty. (Source: Basic and Clinical Andrology)
Source: Basic and Clinical Andrology - December 14, 2021 Category: Urology & Nephrology Source Type: research
In vitro effects of cannabidiol and its main metabolites in mouse and human Sertoli cells
In this study, we used TM4, an immortalized mouse Sertoli cell line, and primary human Sertoli cells to evaluate the toxicities of CBD and its main metabolites, 7-carboxy-CBD and 7-hydroxy-CBD. CBD induced concentration- and time-dependent cytotoxicity in mouse and human Sertoli cells, which mainly resulted from the inhibition of the G1/S-phase cell cycle transition. CBD also inhibited DNA synthesis and downregulated key cell cycle proteins. Moreover, CBD reduced the mRNA and protein levels of a functional marker, Wilms' tumor 1. Similar to CBD, 7-carboxy-CBD and 7-hydroxy-CBD inhibited cellular proliferation and decreased...
Source: Food and Chemical Toxicology - December 6, 2021 Category: Food Science Authors: Yuxi Li Qiangen Wu Xilin Li Linda S Von Tungeln Frederick A Beland Dayton Petibone Lei Guo Patrick Cournoyer Supratim Choudhuri Si Chen Source Type: research
In vitro effects of cannabidiol and its main metabolites in mouse and human Sertoli cells
In this study, we used TM4, an immortalized mouse Sertoli cell line, and primary human Sertoli cells to evaluate the toxicities of CBD and its main metabolites, 7-carboxy-CBD and 7-hydroxy-CBD. CBD induced concentration- and time-dependent cytotoxicity in mouse and human Sertoli cells, which mainly resulted from the inhibition of the G1/S-phase cell cycle transition. CBD also inhibited DNA synthesis and downregulated key cell cycle proteins. Moreover, CBD reduced the mRNA and protein levels of a functional marker, Wilms' tumor 1. Similar to CBD, 7-carboxy-CBD and 7-hydroxy-CBD inhibited cellular proliferation and decreased...
Source: Food and Chemical Toxicology - December 6, 2021 Category: Food Science Authors: Yuxi Li Qiangen Wu Xilin Li Linda S Von Tungeln Frederick A Beland Dayton Petibone Lei Guo Patrick Cournoyer Supratim Choudhuri Si Chen Source Type: research
In vitro effects of cannabidiol and its main metabolites in mouse and human Sertoli cells
In this study, we used TM4, an immortalized mouse Sertoli cell line, and primary human Sertoli cells to evaluate the toxicities of CBD and its main metabolites, 7-carboxy-CBD and 7-hydroxy-CBD. CBD induced concentration- and time-dependent cytotoxicity in mouse and human Sertoli cells, which mainly resulted from the inhibition of the G1/S-phase cell cycle transition. CBD also inhibited DNA synthesis and downregulated key cell cycle proteins. Moreover, CBD reduced the mRNA and protein levels of a functional marker, Wilms' tumor 1. Similar to CBD, 7-carboxy-CBD and 7-hydroxy-CBD inhibited cellular proliferation and decreased...
Source: Food and Chemical Toxicology - December 6, 2021 Category: Food Science Authors: Yuxi Li Qiangen Wu Xilin Li Linda S Von Tungeln Frederick A Beland Dayton Petibone Lei Guo Patrick Cournoyer Supratim Choudhuri Si Chen Source Type: research
Protein kinase A drives paracrine crisis and WNT4-dependent testis tumor in Carney complex
Large-cell calcifying Sertoli cell tumors (LCCSCTs) are among the most frequent lesions occurring in male Carney complex (CNC) patients. Although they constitute a key diagnostic criterion for this rare multiple neoplasia syndrome resulting from inactivating mutations of the tumor suppressor PRKAR1A, leading to unrepressed PKA activity, LCCSCT pathogenesis and origin remain elusive. Mouse models targeting Prkar1a inactivation in all somatic populations or separately in each cell type were generated to decipher the molecular and paracrine networks involved in the induction of CNC testis lesions. We demonstrate that the Prka...
Source: Journal of Clinical Investigation - December 1, 2021 Category: Biomedical Science Authors: Cyril Djari, Isabelle Sahut-Barnola, Amandine Septier, Ingrid Plotton, Nathanaëlle Montanier, Damien Dufour, Adrien Levasseur, James Wilmouth Jr., Jean-Christophe Pointud, Fabio R. Faucz, Crystal Kamilaris, Antoine-Guy Lopez, Florian Guillou, Amanda Swai Source Type: research
The relationship between testicular tumour characteristics and azoospermia: a systematic review
Int J Impot Res. 2021 Nov 6. doi: 10.1038/s41443-021-00492-x. Online ahead of print.ABSTRACTSubfertility is a risk factor for testicular cancers (TT), and conversely, TT may induce subfertility due to local and regional toxic effects. We aimed to identify the association between TT characteristics and pre-orchidectomy azoospermia. A systematic review of the literature was performed according to the PRISMA checklist. Overall, eight non-randomised studies involving 469 men with TT (azoospermia, n = 57; no azoospermia n = 412) were included in the qualitative analysis. Bilateral TT (12.3% vs 2.9% in non-azoospermia), non-semi...
Source: Cell Research - November 7, 2021 Category: Cytology Authors: Karl H Pang Nadir I Osman Asif Muneer Hussain M Alnajjar Source Type: research
Peutz-Jeghers syndrome: Skin manifestations and endocrine anomalies (Review)
Exp Ther Med. 2021 Dec;22(6):1387. doi: 10.3892/etm.2021.10823. Epub 2021 Sep 29.ABSTRACTPeutz-Jeghers syndrome (PJS), a rare autosomal dominant serine/threonine kinase 11 (STK11)/ liver kinase B1 (LKB1) gene-related genodermatosis, is characterized by oral hyperpigmentation (OHP); multiple gastro-intestinal mucosal benign hamartomatous polyps causing local bleeding, occlusion, intussusception, post-resection small bowel syndrome, associated increased risk of small intestinal cancer (incidence during the third decade); and 76% cumulative higher risk than the global population of developing non-gastrointestinal tumors (fema...
Source: Experimental and Therapeutic Medicine - October 15, 2021 Category: General Medicine Authors: Florica Sandru Aida Petca Mihai Cristian Dumitrascu Razvan-Cosmin Petca Mara Carsote Source Type: research
Dermatological and endocrine elements in Carney complex (Review)
Exp Ther Med. 2021 Nov;22(5):1313. doi: 10.3892/etm.2021.10748. Epub 2021 Sep 17.ABSTRACTCarney complex (CΝC) is a very rare, autosomal dominant, hereditary syndrome. Seventy percent of individuals with CNC have germline inactivating or deleting mutations of the CNC1 gene [currently known as protein kinase cAMP-dependent type I regulatory subunit α (PRKAR1A), located at the 17q22-24 chromosome level], with 30% of cases presenting with phosphodiesterase gene mutations. A member of the lentiginosis family, dermatological features include: skin pigmentation, cutaneous/mucosal myxomas, usually diagnosed by the age of 20 year...
Source: Experimental and Therapeutic Medicine - October 11, 2021 Category: General Medicine Authors: Florica Sandru Mihai Cristian Dumitrascu Aida Petca Mara Carsote Razvan-Cosmin Petca Diana Loreta Paun Source Type: research
Changes in histology, protein expression, and autophagy in dairy goat testes during non-breeding season
In this study, adult male Guanzhong dairy goats (10-12 months old) were used. Testis size, semen quality, hormone level, apoptosis of germ cells, and autophagy of Sertoli cells were analyzed in dairy goats during the breeding (October) and non-breeding (April) seasons. We found that, during the non-breeding season for dairy goats, semen quality, follicle-stimulating hormone (FSH) levels, and testosterone levels were reduced, and the number of apoptotic germ cells increased. The proliferation with decrease activity of germ cells in dairy goat during the non-breeding season was significantly affected. However, the testis siz...
Source: Biology of Reproduction - September 1, 2021 Category: Reproduction Medicine Authors: Huaming Xi Fa Ren Yu Li Yeqing Du Liqiang Wang Jianhong Hu Source Type: research
Changes in histology, protein expression, and autophagy in dairy goat testes during non-breeding season
In this study, adult male Guanzhong dairy goats (10-12 months old) were used. Testis size, semen quality, hormone level, apoptosis of germ cells, and autophagy of Sertoli cells were analyzed in dairy goats during the breeding (October) and non-breeding (April) seasons. We found that, during the non-breeding season for dairy goats, semen quality, follicle-stimulating hormone (FSH) levels, and testosterone levels were reduced, and the number of apoptotic germ cells increased. The proliferation with decrease activity of germ cells in dairy goat during the non-breeding season was significantly affected. However, the testis siz...
Source: Reproductive Biology - September 1, 2021 Category: Reproduction Medicine Authors: Huaming Xi Fa Ren Yu Li Yeqing Du Liqiang Wang Jianhong Hu Source Type: research
microRNAs in the pathogenesis of non-obstructive azoospermia: the underlying mechanisms and therapeutic potentials
Syst Biol Reprod Med. 2021 Aug 6:1-17. doi: 10.1080/19396368.2021.1951890. Online ahead of print.ABSTRACTmiRNAs are involved in different biological processes, including proliferation, differentiation, and apoptosis. Interestingly, 38% of the X chromosome-linked miRNAs are testis-specific and have crucial roles in regulating the renewal and cell cycle of spermatogonial stem cells. Previous studies demonstrated that abnormal expression of spermatogenesis-related miRNAs could lead to nonobstructive azoospermia (NOA). Moreover, differential miRNAs expression in seminal plasma of NOA patients has been reported compared to norm...
Source: Systems Biology in Reproductive Medicine - August 6, 2021 Category: Reproduction Medicine Authors: Yeganeh Rastgar Rezaei Reza Zarezadeh Saba Nikanfar Hajar Oghbaei Nahideh Nazdikbin Zahra Bahrami-Asl Nosratollah Zarghami Yadollah Ahmadi Amir Fattahi Mohammad Nouri Ralf Dittrich Source Type: research
