Corrigendum to: Association of polymorphisms in MALAT1 with the risk of endometriosis in Southern Chinese women
Biol Reprod. 2021 Feb 26:ioab030. doi: 10.1093/biolre/ioab030. Online ahead of print.NO ABSTRACTPMID:33636724 | DOI:10.1093/biolre/ioab030 (Source: Biology of Reproduction)
Source: Biology of Reproduction - February 26, 2021 Category: Reproduction Medicine Authors: Guange Chen Mingyao Zhang Zongwen Liang Sailing Chen Feng Chen Jiawei Zhu Manman Zhao Chaoyi Xu Jing He Wenfeng Hua Ping Duan Source Type: research

Corrigendum to: Association of polymorphisms in MALAT1 with the risk of endometriosis in Southern Chinese women
Biol Reprod. 2021 Feb 26:ioab030. doi: 10.1093/biolre/ioab030. Online ahead of print.NO ABSTRACTPMID:33636724 | DOI:10.1093/biolre/ioab030 (Source: Biology of Reproduction)
Source: Biology of Reproduction - February 26, 2021 Category: Reproduction Medicine Authors: Guange Chen Mingyao Zhang Zongwen Liang Sailing Chen Feng Chen Jiawei Zhu Manman Zhao Chaoyi Xu Jing He Wenfeng Hua Ping Duan Source Type: research

Corrigendum to: Association of polymorphisms in MALAT1 with the risk of endometriosis in Southern Chinese women
Biol Reprod. 2021 Feb 26:ioab030. doi: 10.1093/biolre/ioab030. Online ahead of print.NO ABSTRACTPMID:33636724 | DOI:10.1093/biolre/ioab030 (Source: Biology of Reproduction)
Source: Biology of Reproduction - February 26, 2021 Category: Reproduction Medicine Authors: Guange Chen Mingyao Zhang Zongwen Liang Sailing Chen Feng Chen Jiawei Zhu Manman Zhao Chaoyi Xu Jing He Wenfeng Hua Ping Duan Source Type: research

Corrigendum to: Association of polymorphisms in MALAT1 with the risk of endometriosis in Southern Chinese women
Biol Reprod. 2021 Feb 26:ioab030. doi: 10.1093/biolre/ioab030. Online ahead of print.NO ABSTRACTPMID:33636724 | DOI:10.1093/biolre/ioab030 (Source: Biology of Reproduction)
Source: Biology of Reproduction - February 26, 2021 Category: Reproduction Medicine Authors: Guange Chen Mingyao Zhang Zongwen Liang Sailing Chen Feng Chen Jiawei Zhu Manman Zhao Chaoyi Xu Jing He Wenfeng Hua Ping Duan Source Type: research

Mutant Zp1 impedes incorporation of ZP3 and ZP4 in the zona pellucida, resulting in zona absence and female infertility in rats
Biol Reprod. 2021 Feb 24:ioab025. doi: 10.1093/biolre/ioab025. Online ahead of print.ABSTRACTThe zona pellucida (ZP) plays vital roles in reproductive processes including oogenesis, fertilization and preimplantation development. Both human and rat ZP consist of four glycoproteins, called ZP1, ZP2, ZP3 and ZP4. Our previous research reported a novel Zp1 mutation in cases of human infertility, associated with an abnormal phenotype involving the absence of the zona pellucida. Here, we developed a homologous rat strain to investigate the pathogenic effect. The ovaries of homozygous (Zp1MT/MT) females possessed both growing and...
Source: Biology of Reproduction - February 24, 2021 Category: Reproduction Medicine Authors: Chao Lv Hua-Lin Huang Da-Jing Yi Tian-Liu Peng Hang-Jing Tan Ru-Ping Quan Hong-Wen Deng Hong-Mei Xiao Source Type: research

Soluble fms-like tyrosine kinase-1 and angiotensin2 target CALCB family peptides in maternal vascular smooth muscle cells in pregnancy
Biol Reprod. 2021 Feb 24:ioab026. doi: 10.1093/biolre/ioab026. Online ahead of print.ABSTRACTEffect of fms-like tyrosine kinase (sFLT-1) and angiotensin2 (Ang2) on the function of calcitonin gene-related peptide (CALCB), adrenomedullin (ADM) and adrenomedullin2 (ADM2) in vascular smooth muscle cell, and the effect of preeclampsia (PE) on the sensitivity of omental artery (OA) for these peptides is not known in human pregnancy.OBJECTIVE: Identify the effect of sFLT-1 and Ang2 on the function of CALCB family peptides in OA smooth muscle cells (OASMC) and assess the sensitivity of OA for these peptides in PE and normotensive ...
Source: Biology of Reproduction - February 24, 2021 Category: Reproduction Medicine Authors: Madhu Chauhan Ancizar Betancourt Meena Balakrishnan Akansha Mishra Karin Fox Michael Belfort Chandra Yallampalli Source Type: research

Carryover effect of atrazine and its metabolite-from treated bovine spermatozoa to the embryo's transcriptome
Biol Reprod. 2021 Feb 24:ioab027. doi: 10.1093/biolre/ioab027. Online ahead of print.ABSTRACTAtrazine (ATZ) is an extensively used herbicide and ubiquitous environmental contaminant. ATZ and its metabolite, diaminochlorotriazine (DACT), cause several cellular and functional alterations in spermatozoa. We aimed to examine the effect of ATZ/DACT on spermatozoon DNA integrity, fertilization competence, embryonic development and transcriptome profile of in-vitro-produced embryos derived from fertilization with pre-exposed sperm. Bovine spermatozoa exposed to ATZ (0.1 or 1 μM) or DACT (1 or 10 μM) during in-vitro capacita...
Source: Biology of Reproduction - February 24, 2021 Category: Reproduction Medicine Authors: A Komsky-Elbaz D Kalo Z Roth Source Type: research

Cyclic AMP links luteinizing hormone signaling to dephosphorylation and inactivation of the NPR2 guanylyl cyclase in ovarian follicles
Biol Reprod. 2021 Feb 24:ioab029. doi: 10.1093/biolre/ioab029. Online ahead of print.NO ABSTRACTPMID:33624759 | DOI:10.1093/biolre/ioab029 (Source: Biology of Reproduction)
Source: Biology of Reproduction - February 24, 2021 Category: Reproduction Medicine Authors: Jeremy R Egbert Jerid W Robinson Tracy F Uliasz Lincoln R Potter Laurinda A Jaffe Source Type: research

Novel Mineral Regulatory Pathways in Ovine Pregnancy: I. Phosphate, Klotho Signaling, and Sodium Dependent Phosphate Transporters
This study aimed to identify phosphate regulatory pathways in ovine endometria and placentae throughout gestation. Suffolk ewes were bred with fertile rams upon visual detection of estrus (Day 0). On Days 9, 12, 17, 30, 70, 90, 110, and 125 of pregnancy (n = 3-14/Day), ewes were euthanized and hysterectomized. Phosphate abundance varied across gestational days in uterine flushings, allantoic fluid, and homogenized endometria and placentae (P
Source: Biology of Reproduction - February 24, 2021 Category: Reproduction Medicine Authors: Claire Stenhouse Katherine M Halloran Makenzie G Newton Dana Gaddy Larry J Suva Fuller W Bazer Source Type: research

Mutant Zp1 impedes incorporation of ZP3 and ZP4 in the zona pellucida, resulting in zona absence and female infertility in rats
Biol Reprod. 2021 Feb 24:ioab025. doi: 10.1093/biolre/ioab025. Online ahead of print.ABSTRACTThe zona pellucida (ZP) plays vital roles in reproductive processes including oogenesis, fertilization and preimplantation development. Both human and rat ZP consist of four glycoproteins, called ZP1, ZP2, ZP3 and ZP4. Our previous research reported a novel Zp1 mutation in cases of human infertility, associated with an abnormal phenotype involving the absence of the zona pellucida. Here, we developed a homologous rat strain to investigate the pathogenic effect. The ovaries of homozygous (Zp1MT/MT) females possessed both growing and...
Source: Biology of Reproduction - February 24, 2021 Category: Reproduction Medicine Authors: Chao Lv Hua-Lin Huang Da-Jing Yi Tian-Liu Peng Hang-Jing Tan Ru-Ping Quan Hong-Wen Deng Hong-Mei Xiao Source Type: research

Soluble fms-like tyrosine kinase-1 and angiotensin2 target CALCB family peptides in maternal vascular smooth muscle cells in pregnancy
Biol Reprod. 2021 Feb 24:ioab026. doi: 10.1093/biolre/ioab026. Online ahead of print.ABSTRACTEffect of fms-like tyrosine kinase (sFLT-1) and angiotensin2 (Ang2) on the function of calcitonin gene-related peptide (CALCB), adrenomedullin (ADM) and adrenomedullin2 (ADM2) in vascular smooth muscle cell, and the effect of preeclampsia (PE) on the sensitivity of omental artery (OA) for these peptides is not known in human pregnancy.OBJECTIVE: Identify the effect of sFLT-1 and Ang2 on the function of CALCB family peptides in OA smooth muscle cells (OASMC) and assess the sensitivity of OA for these peptides in PE and normotensive ...
Source: Biology of Reproduction - February 24, 2021 Category: Reproduction Medicine Authors: Madhu Chauhan Ancizar Betancourt Meena Balakrishnan Akansha Mishra Karin Fox Michael Belfort Chandra Yallampalli Source Type: research

Carryover effect of atrazine and its metabolite-from treated bovine spermatozoa to the embryo's transcriptome
Biol Reprod. 2021 Feb 24:ioab027. doi: 10.1093/biolre/ioab027. Online ahead of print.ABSTRACTAtrazine (ATZ) is an extensively used herbicide and ubiquitous environmental contaminant. ATZ and its metabolite, diaminochlorotriazine (DACT), cause several cellular and functional alterations in spermatozoa. We aimed to examine the effect of ATZ/DACT on spermatozoon DNA integrity, fertilization competence, embryonic development and transcriptome profile of in-vitro-produced embryos derived from fertilization with pre-exposed sperm. Bovine spermatozoa exposed to ATZ (0.1 or 1 μM) or DACT (1 or 10 μM) during in-vitro capacita...
Source: Biology of Reproduction - February 24, 2021 Category: Reproduction Medicine Authors: A Komsky-Elbaz D Kalo Z Roth Source Type: research

Cyclic AMP links luteinizing hormone signaling to dephosphorylation and inactivation of the NPR2 guanylyl cyclase in ovarian follicles
Biol Reprod. 2021 Feb 24:ioab029. doi: 10.1093/biolre/ioab029. Online ahead of print.NO ABSTRACTPMID:33624759 | DOI:10.1093/biolre/ioab029 (Source: Biology of Reproduction)
Source: Biology of Reproduction - February 24, 2021 Category: Reproduction Medicine Authors: Jeremy R Egbert Jerid W Robinson Tracy F Uliasz Lincoln R Potter Laurinda A Jaffe Source Type: research

Novel Mineral Regulatory Pathways in Ovine Pregnancy: I. Phosphate, Klotho Signaling, and Sodium Dependent Phosphate Transporters
This study aimed to identify phosphate regulatory pathways in ovine endometria and placentae throughout gestation. Suffolk ewes were bred with fertile rams upon visual detection of estrus (Day 0). On Days 9, 12, 17, 30, 70, 90, 110, and 125 of pregnancy (n = 3-14/Day), ewes were euthanized and hysterectomized. Phosphate abundance varied across gestational days in uterine flushings, allantoic fluid, and homogenized endometria and placentae (P
Source: Biology of Reproduction - February 24, 2021 Category: Reproduction Medicine Authors: Claire Stenhouse Katherine M Halloran Makenzie G Newton Dana Gaddy Larry J Suva Fuller W Bazer Source Type: research

Role of Zinc in Female Reproduction
Biol Reprod. 2021 Feb 17:ioab023. doi: 10.1093/biolre/ioab023. Online ahead of print.ABSTRACTZinc is a critical component in a number of conserved processes that regulate female germ cell growth, fertility, and pregnancy. During follicle development a sufficient intracellular concentration of zinc in the oocyte maintains meiotic arrest at prophase I until the germ cell is ready to undergo maturation. An adequate supply of zinc is necessary for the oocyte to form a fertilization-competent egg as dietary zinc deficiency (ZD) or chelation of zinc disrupts maturation and reduces oocyte quality. Following sperm fusion to the eg...
Source: Biology of Reproduction - February 18, 2021 Category: Reproduction Medicine Authors: T B Garner J M Hester A Carothers Francisco J Diaz Source Type: research

Role of Zinc in Female Reproduction
Biol Reprod. 2021 Feb 17:ioab023. doi: 10.1093/biolre/ioab023. Online ahead of print.ABSTRACTZinc is a critical component in a number of conserved processes that regulate female germ cell growth, fertility, and pregnancy. During follicle development a sufficient intracellular concentration of zinc in the oocyte maintains meiotic arrest at prophase I until the germ cell is ready to undergo maturation. An adequate supply of zinc is necessary for the oocyte to form a fertilization-competent egg as dietary zinc deficiency (ZD) or chelation of zinc disrupts maturation and reduces oocyte quality. Following sperm fusion to the eg...
Source: Biology of Reproduction - February 18, 2021 Category: Reproduction Medicine Authors: T B Garner J M Hester A Carothers Francisco J Diaz Source Type: research

Role of Zinc in Female Reproduction.
Abstract Zinc is a critical component in a number of conserved processes that regulate female germ cell growth, fertility, and pregnancy. During follicle development a sufficient intracellular concentration of zinc in the oocyte maintains meiotic arrest at prophase I until the germ cell is ready to undergo maturation. An adequate supply of zinc is necessary for the oocyte to form a fertilization-competent egg as dietary zinc deficiency (ZD) or chelation of zinc disrupts maturation and reduces oocyte quality. Following sperm fusion to the egg to initiate the acrosomal reaction, a quick release of zinc, known as the...
Source: Biology of Reproduction - February 17, 2021 Category: Reproduction Medicine Authors: Garner TB, Hester JM, Carothers A, Diaz FJ Tags: Biol Reprod Source Type: research

Conceptus metabolomic profiling reveals stage-specific phenotypes leading up to pregnancy recognition in cattle.
gan P Abstract Reproductive efficiency in livestock is a major driver of sustainable food production. The poorly-understood process of ruminant conceptus elongation (a) prerequisites maternal pregnancy recognition, (b) is essential to successful pregnancy establishment, and (c) coincides with a period of significant conceptus mortality. Conceptuses at five key developmental stages between Days 8-16 were recovered and cultured in vitro for 6 h prior to conditioned media analysis by untargeted ultrahigh-performance liquid chromatography tandem mass spectroscopy. This global temporal biochemical interrogation of...
Source: Biology of Reproduction - February 16, 2021 Category: Reproduction Medicine Authors: Simintiras CA, Sánchez JM, McDonald M, O'Callaghan E, Aburima AA, Lonergan P Tags: Biol Reprod Source Type: research

METTL3-mediated m6A methylation negatively modulates autophagy to support porcine blastocyst development.
Abstract N6-methyladenosine (m6A) catalyzed by METTL3 regulates the maternal-to-zygotic transition in zebrafish and mice. However, the role and mechanism of METTL3-mediated m6A methylation in blastocyst development remains unclear. Here, we show that METTL3-mediated m6A methylation sustains porcine blastocyst development via negatively modulating autophagy. We found that reduced m6A levels triggered by METTL3 knockdown caused embryonic arrest during morula-blastocyst transition and developmental defects in trophectoderm cells. Intriguingly, overexpression of METTL3 in early embryos resulted in increased m6A levels...
Source: Biology of Reproduction - February 16, 2021 Category: Reproduction Medicine Authors: Cao Z, Zhang L, Hong R, Li Y, Wang Y, Qi X, Ning W, Gao D, Xu T, Ma Y, Yu T, Knott JG, Sathanawongs A, Zhang Y Tags: Biol Reprod Source Type: research

L-amino acid oxidase 1 in sperm is associated with reproductive performance in male mice and bulls.
Abstract Sperm quality is an important indicator of male fertility, and a suitable biomarker enables the selection of high-quality spermatozoa. We previously found that L-amino acid oxidase encoded by the Lao1 gene, exerts biological roles in the mammary gland and brain by converting specific L-amino acids into keto acids, ammonia, and hydrogen peroxide (H2O2). Here, we describe the role of Lao1 in male reproduction. Lao1-deficient (Lao1-/-) male mice generated fewer pregnant embryos and pups as well as lower ratios of fertilized oocytes even ovulated number was not different, suggesting that male subfertility cau...
Source: Biology of Reproduction - February 16, 2021 Category: Reproduction Medicine Authors: Zhang H, Liu H, Kataoka S, Kinukawa M, Uchiyama K, Kambe J, Watanabe G, Jin W, Nagaoka K Tags: Biol Reprod Source Type: research

Maternal Cripto is required for proper uterine decidualization and peri-implantation uterine remodeling.
Abstract Cripto encodes for a cell surface receptor whose role in embryonic development and stem cell maintenance has been studied. Cripto mRNA and protein have been detected in the human uterus at all stages of the menstrual cycle. To date, there is not much known about Cripto's role in female reproduction. As Cripto null Knockout (KO) is embryonic lethal, we created a conditional KO (cKO) mouse model in which Cripto is deleted only in the reproductive tissues using a Cre-loxP system. Pregnancy rate and number of pups per litter were evaluated as general fertility indices. We observed a significant decrease in pr...
Source: Biology of Reproduction - February 16, 2021 Category: Reproduction Medicine Authors: Shafiei S, Farah O, Dufort D Tags: Biol Reprod Source Type: research

Melatonergic systems of AANAT, melatonin, and its receptor MT2 in the corpus luteum are essential for reproductive success in mammals †.
Melatonergic systems of AANAT, melatonin, and its receptor MT2 in the corpus luteum are essential for reproductive success in mammals†. Biol Reprod. 2021 Feb 11;104(2):430-444 Authors: Wang J, Zhu T, Ma X, Wang Y, Liu J, Li G, Liu Y, Ji P, Zhang Z, Zhang L, Liu G Abstract Corpus luteum (CL) plays a critical role in mammalian reproductive physiology. Its dysfunction will lead to infertility or habitual abortion. In the current study, by use of melatonin specific membrane receptor 2 (MT2) knocking out (KO) mice model combined with RNA-Seq, immunohistochemistry, and immunofluorescence analyses, th...
Source: Biology of Reproduction - February 11, 2021 Category: Reproduction Medicine Authors: Wang J, Zhu T, Ma X, Wang Y, Liu J, Li G, Liu Y, Ji P, Zhang Z, Zhang L, Liu G Tags: Biol Reprod Source Type: research

Evidence and manipulation of O-GlcNAcylation in granulosa cells of bovine antral follicles.
Abstract Glucose is a preferred energy substrate for metabolism by bovine granulosa cells (GCs). O-linked N-acetylglucosaminylation (O-GlcNAcylation), is a product of glucose metabolism that occurs as the hexosamine biosynthesis pathway (HBP) shunts O-GlcNAc sugars to serine and threonine residues of proteins. O-GlcNAcylation through the HBP is considered a nutrient sensing mechanism that regulates many cellular processes. Yet little is known of its importance in GCs. Here, O-GlcNAcylation in GCs and its effects on GC proliferation were determined. Bovine ovaries from an abattoir, staged to the mid-to-late estrous...
Source: Biology of Reproduction - February 8, 2021 Category: Reproduction Medicine Authors: Maucieri AM, Townson DH Tags: Biol Reprod Source Type: research

Activation of bovine oocytes by protein synthesis inhibitors: New findings on the role of MPF/MAPKs †.
Activation of bovine oocytes by protein synthesis inhibitors: New findings on the role of MPF/MAPKs†. Biol Reprod. 2021 Feb 08;: Authors: Valencia C, Pérez FA, Matus C, Felmer R, Arias ME Abstract The present study evaluated the mechanism by which protein synthesis inhibitors activate bovine oocytes. The aim was to analyze the dynamics of MPF and MAPKs. MII oocytes were activated with ionomycin (Io), ionomycin+anisomycin (ANY) and ionomycin+cycloheximide (CHX) and by in vitro fertilization (IVF). The expression of cyclin B1, p-CDK1, p-ERK1/2, p-JNK and p-P38 were evaluated by immunodetec...
Source: Biology of Reproduction - February 8, 2021 Category: Reproduction Medicine Authors: Valencia C, Pérez FA, Matus C, Felmer R, Arias ME Tags: Biol Reprod Source Type: research

Current Progress, Challenges, and Future Prospects of Testis Organoids.
This article briefly reviews the current progress in the development of scaffold-based testis organoids while focusing on the scaffolding biomaterials (hydrogels), cell sources, and scaffolding approaches. Key challenges in current organoid studies are also discussed along with recommendations for future research. PMID: 33550399 [PubMed - as supplied by publisher] (Source: Biology of Reproduction)
Source: Biology of Reproduction - February 8, 2021 Category: Reproduction Medicine Authors: Cham TC, Chen X, Honaramooz A Tags: Biol Reprod Source Type: research

Impaired decidualization of human endometrial stromal cells from women with adenomyosis.
Abstract Differentiation of endometrial stromal cells (ESCs) into secretory decidualized cells (dESCs) is essential for embryo implantation. Adenomyosis is a common benign gynecological disease that causes infertility. However, whether adenomyosis affects decidualization of human endometrial stromal cells is elusive. Primary eutopic ESCs were obtained from patients with adenomyosis (n = 9) and women with non-endometrial diseases (n = 12). We determined the capacity of decidualization of human ESCs by qRT-PCR, Edu proliferation assay, cytokine array and ELISA assay. We found that the expression ...
Source: Biology of Reproduction - February 2, 2021 Category: Reproduction Medicine Authors: Peng Y, Jin Z, Liu H, Xu C Tags: Biol Reprod Source Type: research

Sphingosine-1-phosphate and its mimetic FTY720 do not protect against radiation-induced ovarian fibrosis in the nonhuman primate.
Abstract Oocytes are highly radiosensitive, so agents that prevent radiation-induced ovarian follicle destruction are important fertility preservation strategies. A previous study in rhesus macaques demonstrated that ovarian treatment with anti-apoptotic agents, sphingosine-1-phosphate (S1P) and FTY720, its long-acting mimetic, preserved follicles following a single dose of 15 Gy X-ray radiation, and live offspring were obtained from FTY720-treated animals. However, it is unknown whether these anti-apoptotic agents also protected the ovarian stroma from late effects of radiation, including vascular damage and...
Source: Biology of Reproduction - February 2, 2021 Category: Reproduction Medicine Authors: Amargant F, Manuel SL, Larmore MJ, Johnson BW, Lawson M, Pritchard MT, Zelinski MB, Duncan FE Tags: Biol Reprod Source Type: research

Rbm46, a novel germ cell specific factor, modulates meiotic progression and spermatogenesis.
Abstract nesis but few has been functionally verified. Here, we identified one member, Rbm46 and investigated its expression and role during zebrafish reproduction. We found that is maternally provided and specifically expressed in the germ cells of gonadal tissues using in situ hybridization, RT-PCR and qRT-PCR. Utilizing TALEN (transcription activator-like effector nuclease) techniques, two independent rbm46 mutant zebrafish lines were generated. Specific disruption of rbm46 resulted in masculinization and infertility in the mutants. Although the spermatogonia appeared grossly normal, spermatogenesis was impaire...
Source: Biology of Reproduction - February 2, 2021 Category: Reproduction Medicine Authors: Dai X, Cheng X, Huang J, Gao Y, Wang D, Feng Z, Zhai G, Lou Q, He J, Wang Z, Yin Z Tags: Biol Reprod Source Type: research

Effects of the endocrine disruptor vinclozolin in male reproduction: a systematic review and meta-analysis.
eia S Abstract Endocrine disrupting chemicals (EDCs) have become an issue of scientific and public discussion. Vinclozolin (VNZ) is a fungicide that competitively antagonizes the binding of natural androgens to their receptor, disturbing the function of tissues that are sensitive to these hormones, as is the case of the male reproductive organs. A systematic review with meta-analyses of rodent studies was conducted to answer the following question: does exposure to VNZ affect sperm parameters and testicular/epididymal weight? The methodology was prespecified according to the Cochrane Handbook for Systematic Review...
Source: Biology of Reproduction - February 2, 2021 Category: Reproduction Medicine Authors: Feijó M, Martins RVL, Socorro S, Pereira L, Correia S Tags: Biol Reprod Source Type: research

Regulation of gene expression in the bovine blastocyst by CSF2 is disrupted by CRISPR/Cas9-mediated deletion of CSF2RA.
, Hansen PJ Abstract Colony stimulating factor 2 (CSF2) functions in the reproductive tract to modulate function of the preimplantation embryo. The β subunit of the CSF2 receptor (CSF2RB) is not expressed in the embryo and signal transduction is therefore different than for myeloid cells where the receptor is composed of α (CSF2RA) and β subunits. Here, we produced embryos in which exons 5 and 6 of CSF2RA were disrupted using the CRISPR/Cas 9 system to test whether CSF2RA signaling was essential for actions of CSF2 in the bovine embryo. Wildtype and CSF2RA knockout embryos were treated with 10 ...
Source: Biology of Reproduction - February 2, 2021 Category: Reproduction Medicine Authors: Xiao Y, Uh K, Negrón-Pérez VM, Haines H, Lee K, Hansen PJ Tags: Biol Reprod Source Type: research

Dynamic erectile responses of a novel penile organ model utilizing two photon excitation microscopy (TPEM) †.
Dynamic erectile responses of a novel penile organ model utilizing two photon excitation microscopy (TPEM)†. Biol Reprod. 2021 Jan 28;: Authors: Hashimoto D, Hirashima T, Yamamura H, Kataoka T, Fujimoto K, Hyuga T, Yoshiki A, Kimura K, Kuroki S, Tachibana M, Suzuki K, Yamamoto N, Morioka S, Sasaki T, Yamada G Abstract Male penis is required to become erect during copulation. In the upper (dorsal) part of penis, the erectile tissue termed corpus cavernosum (CC) plays fundamental roles for erection by regulating the inner blood flow. When blood flows into the CC, the microvascular complex termed ...
Source: Biology of Reproduction - January 28, 2021 Category: Reproduction Medicine Authors: Hashimoto D, Hirashima T, Yamamura H, Kataoka T, Fujimoto K, Hyuga T, Yoshiki A, Kimura K, Kuroki S, Tachibana M, Suzuki K, Yamamoto N, Morioka S, Sasaki T, Yamada G Tags: Biol Reprod Source Type: research

Environmentally relevant mixtures of phthalates and phthalate metabolites differentially alter the cell cycle and apoptosis in mouse neonatal ovaries †.
Environmentally relevant mixtures of phthalates and phthalate metabolites differentially alter the cell cycle and apoptosis in mouse neonatal ovaries†. Biol Reprod. 2021 Jan 28;: Authors: Warner GR, Meling DD, De La Torre KM, Wang K, Flaws JA Abstract Phthalates are a group of chemicals used as additives in various consumer products, medical equipment, and personal care products. Phthalates and their metabolites are consistently detected in humans, indicating widespread and continuous exposure to multiple phthalates. Thus, environmentally relevant mixtures of phthalates and phthalate metabolite...
Source: Biology of Reproduction - January 28, 2021 Category: Reproduction Medicine Authors: Warner GR, Meling DD, De La Torre KM, Wang K, Flaws JA Tags: Biol Reprod Source Type: research

Mitochondrial transfer from iPS cells rescues developmental potential of in vitro fertilized embryos from aging females †.
Mitochondrial transfer from iPS cells rescues developmental potential of in vitro fertilized embryos from aging females†. Biol Reprod. 2021 Jan 28;: Authors: Zhang C, Tao L, Yue Y, Ren L, Zhang Z, Wang X, Tian J, An L Abstract Conventional heterologous mitochondrial replacement therapy is clinically complicated by "tri-parental" ethical concerns and limited source of healthy donor oocytes or zygotes. Autologous mitochondrial transfer is a promising alternative in rescuing poor oocyte quality and impaired embryo developmental potential associated with mitochondrial disorders, including...
Source: Biology of Reproduction - January 28, 2021 Category: Reproduction Medicine Authors: Zhang C, Tao L, Yue Y, Ren L, Zhang Z, Wang X, Tian J, An L Tags: Biol Reprod Source Type: research

Glucose Metabolism Characterization During Mouse In Vitro Maturation Identifies Alterations In Cumulus Cells †.
Glucose Metabolism Characterization During Mouse In Vitro Maturation Identifies Alterations In Cumulus Cells†. Biol Reprod. 2021 Jan 22;: Authors: Akin N, von Mengden L, Herta AC, Billooye K, Leersum J, Cava-Cami B, Saucedo-Cuevas L, Klamt F, Smitz J, Anckaert E Abstract In vitro maturation (IVM) is an assisted reproduction technique with reduced hormone-related side effects. Several attempts to implement IVM in routine practice have failed, primarily due to its relatively low efficiency compared to conventional in vitro fertilization (IVF). Recently, capacitation (CAPA)-IVM, a novel two-step I...
Source: Biology of Reproduction - January 22, 2021 Category: Reproduction Medicine Authors: Akin N, von Mengden L, Herta AC, Billooye K, Leersum J, Cava-Cami B, Saucedo-Cuevas L, Klamt F, Smitz J, Anckaert E Tags: Biol Reprod Source Type: research

Male Fetal Sex Affects Uteroplacental Angiogenesis in Growth Restriction Mouse Model †.
Male Fetal Sex Affects Uteroplacental Angiogenesis in Growth Restriction Mouse Model†. Biol Reprod. 2021 Jan 18;: Authors: Hebert JF, Millar JA, Raghavan R, Romney A, Podrabsky JE, Rennie MY, Felker A, O'Tierney-Ginn P, Morita M, DuPriest EA, Morgan TK Abstract Abnormally increased angiotensin II activity related to maternal angiotensinogen (AGT) genetic variants, or aberrant receptor activation, is associated with small-for-gestational-age (SGA) babies and abnormal uterine spiral artery remodeling in humans. Our group studies a murine AGT gene titration transgenic (TG; 3-copies of the AGT gene...
Source: Biology of Reproduction - January 18, 2021 Category: Reproduction Medicine Authors: Hebert JF, Millar JA, Raghavan R, Romney A, Podrabsky JE, Rennie MY, Felker A, O'Tierney-Ginn P, Morita M, DuPriest EA, Morgan TK Tags: Biol Reprod Source Type: research

Metabolomic differences in blastocoel and uterine fluids collected in vivo by ultrasound biomicroscopy on rabbit embryos †.
Metabolomic differences in blastocoel and uterine fluids collected in vivo by ultrasound biomicroscopy on rabbit embryos†. Biol Reprod. 2021 Jan 18;: Authors: Calderari S, Daniel N, Mourier E, Richard C, Dahirel M, Lager F, Marchiol C, Renault G, Gatien J, Nadal-Desbarats L, Chavatte-Palmer P, Duranthon V Abstract The success of embryo development and implantation depends in part on the environment in which the embryo evolves. However, the composition of the uterine fluid surrounding the embryo in the peri-implantation period remains poorly studied. In this work, we aimed to develop a new strat...
Source: Biology of Reproduction - January 18, 2021 Category: Reproduction Medicine Authors: Calderari S, Daniel N, Mourier E, Richard C, Dahirel M, Lager F, Marchiol C, Renault G, Gatien J, Nadal-Desbarats L, Chavatte-Palmer P, Duranthon V Tags: Biol Reprod Source Type: research

Tex55 encodes a conserved putative A-kinase anchoring protein dispensable for male fertility in the mouse.
PMID: 33458765 [PubMed - as supplied by publisher] (Source: Biology of Reproduction)
Source: Biology of Reproduction - January 16, 2021 Category: Reproduction Medicine Authors: Jamin SP, Petit FG, Demini L, Primig M Tags: Biol Reprod Source Type: research

Gene-expression profile and postpartum transition of bovine endometrial side population cells †.
Gene-expression profile and postpartum transition of bovine endometrial side population cells†. Biol Reprod. 2021 Jan 13;: Authors: Tatebayashi R, Nakamura S, Minabe S, Furusawa T, Abe R, Kajisa M, Morita Y, Ohkura S, Kimura K, Matsuyama S Abstract The mechanism of bovine endometrial regeneration after parturition remains unclear. Here, we hypothesized that bovine endometrial stem/progenitor cells participate in the postpartum regeneration of the endometrium. Flow cytometry analysis identified the presence of side population (SP) cells among endometrial stromal cells. Endometrial SP cells were ...
Source: Biology of Reproduction - January 13, 2021 Category: Reproduction Medicine Authors: Tatebayashi R, Nakamura S, Minabe S, Furusawa T, Abe R, Kajisa M, Morita Y, Ohkura S, Kimura K, Matsuyama S Tags: Biol Reprod Source Type: research

Differences in the proteome of stallion spermatozoa explain stallion-to-stallion variability in sperm quality post thaw †.
Differences in the proteome of stallion spermatozoa explain stallion-to-stallion variability in sperm quality post thaw†. Biol Reprod. 2021 Jan 12;: Authors: Gaitskell-Phillips G, Martín-Cano FE, Ortiz-Rodríguez JM, Silva-Rodríguez A, Gil MC, Ortega-Ferrusola C, Peña FJ Abstract Although cryopreservation is becoming a common practice in modern equine breeding, there are still a number of unresolved issues limiting its use. One of these is the stallion to stallion variability in the post thaw quality of ejaculates. The identification of stallions and or ejaculates tha...
Source: Biology of Reproduction - January 12, 2021 Category: Reproduction Medicine Authors: Gaitskell-Phillips G, Martín-Cano FE, Ortiz-Rodríguez JM, Silva-Rodríguez A, Gil MC, Ortega-Ferrusola C, Peña FJ Tags: Biol Reprod Source Type: research

Endometrial Receptivity and Embryo Implantation in Carnivores-Commonalities and Differences with Other Mammalian Species.
Abstract Endometrial receptivity and embryo implantation processes are a major point of pregnancy failure in many mammalian species, including humans. Although reproductive biology in many carnivore species remains enigmatic, the few that have been studied so far are invaluable comparative models. The goals of this review are to (1) summarize current data on the mechanisms involved in uterine receptivity and embryo implantation in carnivores including commonalities and differences with other mammalian species and (2) identify research priorities to better understand a key phenomenon in a critical group of mammals....
Source: Biology of Reproduction - January 7, 2021 Category: Reproduction Medicine Authors: Paulson EE, Comizzoli P Tags: Biol Reprod Source Type: research

Toxicant Effects on Mammalian Oocyte Mitochondria.
Abstract Oocyte mitochondria are unique organelles that establish a founder population in primordial germ cells (PGCs). As the oocyte matures in the postnatal mammalian ovary during folliculogenesis it increases exponentially in volume, and the oocyte mitochondria population proliferates to about 100,000 mitochondria per healthy, mature murine oocyte. The health of the mature oocyte and subsequent embryo is highly dependent on the oocyte mitochondria. Mitochondria are especially sensitive to toxic insults, as they are a main source of reactive oxygen species (ROS), they contain their own DNA (mtDNA) that is unprot...
Source: Biology of Reproduction - January 7, 2021 Category: Reproduction Medicine Authors: Malott KF, Luderer U Tags: Biol Reprod Source Type: research

Protein Hormone Fragmentation in Intercellular Signaling: Hormones as Nested Information Systems †.
This study explores the hypothesis that protein hormones are nested information systems in which initial products of gene transcription, and their subsequent protein fragments, before and after secretion and initial target cell action, play additional physiological regulatory roles. The study produced four tools and key results: 1) a problem approach that proceeds, with examples and suggestions for in vivo organismal functional tests for peptide-protein interactions, from proteolytic breakdown prediction to models of hormone fragment modulation of protein-protein binding motifs in unrelated proteins; 2) a catalog of 461 kn...
Source: Biology of Reproduction - January 5, 2021 Category: Reproduction Medicine Authors: Campbell KL, Haspel N, Gath C, Kurniatash N, Nouduri Akkiraju I, Stuffers N, Vadher U Tags: Biol Reprod Source Type: research

Cytoplasmic maturation in human oocytes: an ultrastructural study †.
In conclusion, this work improves our understanding of human oocyte morphology, cytoplasmic maturation, and intracellular factors defining human egg quality. Although this analysis involved spare oocytes completing development in vitro, it provides essential insight into the enigmatic process by which human egg progenitors prepare for fertilization. PMID: 33404651 [PubMed - in process] (Source: Biology of Reproduction)
Source: Biology of Reproduction - January 4, 2021 Category: Reproduction Medicine Authors: Trebichalská Z, Kyjovská D, Kloudová S, Otevřel P, Hampl A, Holubcová Z Tags: Biol Reprod Source Type: research

A transgenic DND1GFP fusion allele reports in vivo expression and RNA binding targets in undifferentiated mouse germ cells.
We present imaging analysis of DND1GFP expression showing that DND1GFP expression is heterogeneous among male germ cells (MGCs) and female germ cells (FGCs). DND1GFP was detected in MGCs throughout fetal life but lost from FGCs at meiotic entry. In postnatal and adult testes, DND1GFP expression correlated with classic markers for the pre-meiotic spermatogonial population. Utilizing the GFP-tag for RNA immunoprecipitation (RIP) analysis in MGCs validated this transgenic as a tool for identifying in vivo transcript targets of DND1. The DND1GFP mouse line is a novel tool for isolation and analysis of embryonic and fetal germ ...
Source: Biology of Reproduction - January 4, 2021 Category: Reproduction Medicine Authors: Ruthig VA, Yokonishi T, Friedersdorf MB, Batchvarova S, Hardy J, Garness JA, Keene JD, Capel B Tags: Biol Reprod Source Type: research

Male-derived copulatory plugs enhance implantation success in female Mus musculus †.
Male-derived copulatory plugs enhance implantation success in female Mus musculus†. Biol Reprod. 2020 Dec 23;: Authors: Michael LS, Ghione C, Urness M, Hobbs A, Sweeney C, Dean MD Abstract Among a wide diversity of sexually reproducing species, male ejaculates coagulate to form what has been termed a copulatory plug. A number of functions have been attributed to copulatory plugs, including, but not limited to, the inhibition of female remating and the promotion of ejaculate movement. Here we demonstrate that copulatory plugs also influence the likelihood of implantation, which occurs roughly fo...
Source: Biology of Reproduction - December 23, 2020 Category: Reproduction Medicine Authors: Michael LS, Ghione C, Urness M, Hobbs A, Sweeney C, Dean MD Tags: Biol Reprod Source Type: research

Implantation failure and embryo loss contribute to subfertility in female mice mutant for chromatin remodeler Cecr2 †.
Implantation failure and embryo loss contribute to subfertility in female mice mutant for chromatin remodeler Cecr2†. Biol Reprod. 2020 Dec 22;: Authors: Norton KA, Niri F, Weatherill CB, Williams CE, Duong K, McDermid HE Abstract Defects in the maternal reproductive system that result in early pregnancy loss are important causes of human female infertility. A wide variety of biological processes are involved in implantation and establishment of a successful pregnancy. While chromatin remodelers have been shown to play an important role in many biological processes, our understanding of the rol...
Source: Biology of Reproduction - December 22, 2020 Category: Reproduction Medicine Authors: Norton KA, Niri F, Weatherill CB, Williams CE, Duong K, McDermid HE Tags: Biol Reprod Source Type: research

Synergistic action of estradiol and PGE2 on endometrial transcriptome in vivo resembles pregnancy effects better than estradiol alone †.
Synergistic action of estradiol and PGE2 on endometrial transcriptome in vivo resembles pregnancy effects better than estradiol alone†. Biol Reprod. 2020 Dec 22;: Authors: Kaczynski P, Bauersachs S, Goryszewska E, Baryla M, Waclawik A Abstract Successful pregnancy establishment in mammals depends on numerous interactions between embryos and the maternal organism. Estradiol-17β (E2) is the primary embryonic signal in the pig, and its importance has been questioned recently. However, E2 is not the only molecule of embryonic origin. In pigs, prostaglandin E2 (PGE2) is abundantly synthesized a...
Source: Biology of Reproduction - December 22, 2020 Category: Reproduction Medicine Authors: Kaczynski P, Bauersachs S, Goryszewska E, Baryla M, Waclawik A Tags: Biol Reprod Source Type: research

Developmental programming of the female reproductive system-a review.
Abstract Exposures to adverse conditions in utero can lead to permanent changes in the structure and function of key physiological systems in the developing fetus, increasing the risk of disease and premature aging in later postnatal life. When considering the systems that could be affected by an adverse gestational environment, the reproductive system of developing female offspring may be particularly important, as changes have the potential to alter both reproductive capacity of the first generation, as well as health of the second generation through changes in the oocyte. The aim of this review is to examine th...
Source: Biology of Reproduction - December 22, 2020 Category: Reproduction Medicine Authors: Yao S, Lopez-Tello J, Sferruzzi-Perri AN Tags: Biol Reprod Source Type: research

Pyometra and estrous cycle modulate the uterine expression of the Kisspeptin system and angiogenic and immune factors in cats.
This study suggests, for the first time, a functional relationship between the Kiss/Kiss1r system and angiogenic and immune mediators in the female genital tract. PMID: 33348359 [PubMed - as supplied by publisher] (Source: Biology of Reproduction)
Source: Biology of Reproduction - December 21, 2020 Category: Reproduction Medicine Authors: Santos LC, Anjos Cordeiro JM, Silva Santana L, Santana LR, Santos BR, Barbosa EM, Silva TQM, Corrêa JMX, Lavor MSL, Silva EB, Melo Ocarino N, Serakides R, Silva JF Tags: Biol Reprod Source Type: research

Insulin-like growth factor 2 is produced by antral follicles and promotes preantral follicle development in macaques.
Abstract Insulin-like growth factors (IGFs) are known for their involvement in endocrine and paracrine regulation of ovarian function. While IGF2 is the predominant circulating and intra-ovarian form of IGFs in primate species, the stage-specific follicular expression, action and regulation of IGF2 are not well defined. Therefore, experiments were conducted to investigate the follicular IGF production in response to steroid hormone regulation and the direct IGF actions on follicular development and function in vitro. Preantral follicles were isolated from rhesus macaque ovaries and cultured to the antral stage in ...
Source: Biology of Reproduction - December 21, 2020 Category: Reproduction Medicine Authors: Tkachenko OY, Wolf S, Lawson MS, Ting AY, Rodrigues JK, Xu F, Bishop CV, Stouffer RL, Xu J Tags: Biol Reprod Source Type: research