Effects of non-inherited ancestral genotypes on offspring phenotypes
Biol Reprod. 2021 Jun 22:ioab120. doi: 10.1093/biolre/ioab120. Online ahead of print.ABSTRACTIt is well established that environmental exposures can modify the profile of heritable factors in an individual's germ cells, ultimately affecting the inheritance of phenotypes in descendants. Similar to exposures, an ancestor's genotype can also affect the inheritance of phenotypes across generations, sometimes in offspring who do not inherit the genetic aberration. This can occur via a variety of prenatal, in utero, or postnatal mechanisms. In this review, we discuss the evidence for this process in mammals, with a focus on exam...
Source: Biology of Reproduction - June 23, 2021 Category: Reproduction Medicine Authors: Sean M Cullen Nora Hassan Matthew Smith-Raska Source Type: research

Evidence for a shift in placental HLA-G allelic dominance and the HLA-G isoform profile during a healthy pregnancy and pre-eclampsia
Biol Reprod. 2021 Jun 22:ioab121. doi: 10.1093/biolre/ioab121. Online ahead of print.ABSTRACTHuman leukocyte antigen (HLA)-G is a non-classical class Ib major expressed by placental trophoblast cells plays a central role in establishing tolerance to the semi-allogeneic fetus and in placentation. HLA-G exists in different soluble or membrane-bound isoforms. Pre-eclampsia, a major cause of fetal and maternal morbidity and mortality, has been linked to insufficient placentation and an altered immune response in pregnancy, including altered HLA-G expression. The 14 bp insertion/deletion polymorphism in the 3' untranslated regi...
Source: Biology of Reproduction - June 23, 2021 Category: Reproduction Medicine Authors: Gry Persson Christina Seefeldt St æhr Freja Syrach Klok Morten Lebech Thomas Vauvert F Hviid Source Type: research

State of ART in the COVID-19 era and consequences on human reproductive system
Biol Reprod. 2021 Jun 22:ioab122. doi: 10.1093/biolre/ioab122. Online ahead of print.ABSTRACTThe outbreak of the COVID-19 virus has created havoc on the socio-economic aspect of the world. With billions of lives affected by this wrecking pandemic, global fertility services were also not left untouched by its impact. The possibility of sexual transmission of SARS-CoV-2 virus, its impact on male and female fertility, pregnancy, its potential teratogenic effect, and handling of gametes in the clinical laboratories were major concerns among reproductive medicine specialists which led down all the reproductive health services i...
Source: Biology of Reproduction - June 23, 2021 Category: Reproduction Medicine Authors: Anadeep Chandi Neelam Jain Source Type: research

Low CLOCK and CRY2 in 2nd trimester human maternal blood and risk of preterm birth: A nested case-control study
We examined the association of 10 core circadian transcripts in maternal blood with spontaneous PTB (sPTB) vs term births using a nested case-control study design. We used a public gene expression dataset (GSE59491), which was nested within the All Our Babies (AOB) study cohort in Canada. Maternal blood was sampled in trimesters 2-3 from women with sPTB (n = 51) and term births (n = 106), matched for 5 demographic variables. In 2nd trimester maternal blood, only CLOCK and CRY2 transcripts were significantly lower in sPTB vs term (p = 0.02 ~ 0.03, FDR
Source: Biology of Reproduction - June 18, 2021 Category: Reproduction Medicine Authors: Guoli Zhou Thu V Duong Eric P Kasten Hanne M Hoffmann Source Type: research

Low CLOCK and CRY2 in 2nd trimester human maternal blood and risk of preterm birth: A nested case-control study
We examined the association of 10 core circadian transcripts in maternal blood with spontaneous PTB (sPTB) vs term births using a nested case-control study design. We used a public gene expression dataset (GSE59491), which was nested within the All Our Babies (AOB) study cohort in Canada. Maternal blood was sampled in trimesters 2-3 from women with sPTB (n = 51) and term births (n = 106), matched for 5 demographic variables. In 2nd trimester maternal blood, only CLOCK and CRY2 transcripts were significantly lower in sPTB vs term (p = 0.02 ~ 0.03, FDR
Source: Biology of Reproduction - June 18, 2021 Category: Reproduction Medicine Authors: Guoli Zhou Thu V Duong Eric P Kasten Hanne M Hoffmann Source Type: research

Low CLOCK and CRY2 in 2nd trimester human maternal blood and risk of preterm birth: A nested case-control study
We examined the association of 10 core circadian transcripts in maternal blood with spontaneous PTB (sPTB) vs term births using a nested case-control study design. We used a public gene expression dataset (GSE59491), which was nested within the All Our Babies (AOB) study cohort in Canada. Maternal blood was sampled in trimesters 2-3 from women with sPTB (n = 51) and term births (n = 106), matched for 5 demographic variables. In 2nd trimester maternal blood, only CLOCK and CRY2 transcripts were significantly lower in sPTB vs term (p = 0.02 ~ 0.03, FDR
Source: Biology of Reproduction - June 18, 2021 Category: Reproduction Medicine Authors: Guoli Zhou Thu V Duong Eric P Kasten Hanne M Hoffmann Source Type: research

Low CLOCK and CRY2 in 2nd trimester human maternal blood and risk of preterm birth: A nested case-control study
We examined the association of 10 core circadian transcripts in maternal blood with spontaneous PTB (sPTB) vs term births using a nested case-control study design. We used a public gene expression dataset (GSE59491), which was nested within the All Our Babies (AOB) study cohort in Canada. Maternal blood was sampled in trimesters 2-3 from women with sPTB (n = 51) and term births (n = 106), matched for 5 demographic variables. In 2nd trimester maternal blood, only CLOCK and CRY2 transcripts were significantly lower in sPTB vs term (p = 0.02 ~ 0.03, FDR
Source: Biology of Reproduction - June 18, 2021 Category: Reproduction Medicine Authors: Guoli Zhou Thu V Duong Eric P Kasten Hanne M Hoffmann Source Type: research

Low CLOCK and CRY2 in 2nd trimester human maternal blood and risk of preterm birth: A nested case-control study
We examined the association of 10 core circadian transcripts in maternal blood with spontaneous PTB (sPTB) vs term births using a nested case-control study design. We used a public gene expression dataset (GSE59491), which was nested within the All Our Babies (AOB) study cohort in Canada. Maternal blood was sampled in trimesters 2-3 from women with sPTB (n = 51) and term births (n = 106), matched for 5 demographic variables. In 2nd trimester maternal blood, only CLOCK and CRY2 transcripts were significantly lower in sPTB vs term (p = 0.02 ~ 0.03, FDR
Source: Biology of Reproduction - June 18, 2021 Category: Reproduction Medicine Authors: Guoli Zhou Thu V Duong Eric P Kasten Hanne M Hoffmann Source Type: research

The molecular mechanisms underlying acrosome biogenesis elucidated by gene-manipulated mice
Biol Reprod. 2021 Jun 15:ioab117. doi: 10.1093/biolre/ioab117. Online ahead of print.ABSTRACTSexual reproduction requires the fusion of two gametes in a multistep and multifactorial process termed fertilization. One of the main steps that ensures successful fertilization is acrosome reaction. The acrosome, a special kind of organelle with a cap-like structure that covers the anterior portion of sperm head, plays a key role in the process. Acrosome biogenesis begins with the initial stage of spermatid development, and it is typically divided into four successive phases: the Golgi phase, cap phase, acrosome phase, and matura...
Source: Biology of Reproduction - June 16, 2021 Category: Reproduction Medicine Authors: Wenfeng Xiong Chunling Shen Zhugang Wang Source Type: research

Metabolo-epigenetics: The interplay of metabolism and epigenetics during early germ cells development
Biol Reprod. 2021 Jun 16:ioab118. doi: 10.1093/biolre/ioab118. Online ahead of print.ABSTRACTMetabolites control epigenetic mechanisms and, conversly, cell metabolism is regulated at the epigenetic level in response to changes in the cellular environnement. In recent years, this metabolo-epigenetic control of gene expression has been implicated in the regulation of multiple stages of embryonic development. The developmental potency of stem cells and their embryonic counterparts is directly determined by metabolic rewiring. Here, we review the current knowledge on the interplay between epigenetics and metabolism in the spec...
Source: Biology of Reproduction - June 16, 2021 Category: Reproduction Medicine Authors: Roxane Verdikt Patrick Allard Source Type: research

The molecular mechanisms underlying acrosome biogenesis elucidated by gene-manipulated mice
Biol Reprod. 2021 Jun 15:ioab117. doi: 10.1093/biolre/ioab117. Online ahead of print.ABSTRACTSexual reproduction requires the fusion of two gametes in a multistep and multifactorial process termed fertilization. One of the main steps that ensures successful fertilization is acrosome reaction. The acrosome, a special kind of organelle with a cap-like structure that covers the anterior portion of sperm head, plays a key role in the process. Acrosome biogenesis begins with the initial stage of spermatid development, and it is typically divided into four successive phases: the Golgi phase, cap phase, acrosome phase, and matura...
Source: Biology of Reproduction - June 16, 2021 Category: Reproduction Medicine Authors: Wenfeng Xiong Chunling Shen Zhugang Wang Source Type: research

Metabolo-epigenetics: The interplay of metabolism and epigenetics during early germ cells development
Biol Reprod. 2021 Jun 16:ioab118. doi: 10.1093/biolre/ioab118. Online ahead of print.ABSTRACTMetabolites control epigenetic mechanisms and, conversly, cell metabolism is regulated at the epigenetic level in response to changes in the cellular environnement. In recent years, this metabolo-epigenetic control of gene expression has been implicated in the regulation of multiple stages of embryonic development. The developmental potency of stem cells and their embryonic counterparts is directly determined by metabolic rewiring. Here, we review the current knowledge on the interplay between epigenetics and metabolism in the spec...
Source: Biology of Reproduction - June 16, 2021 Category: Reproduction Medicine Authors: Roxane Verdikt Patrick Allard Source Type: research

Heritable consequences of paternal nicotine exposure: From phenomena to mechanisms
Biol Reprod. 2021 Jun 14:ioab116. doi: 10.1093/biolre/ioab116. Online ahead of print.ABSTRACTOur understanding of the interactions between genetic and environmental factors in shaping behavioral phenotypes has expanded to include environment-induced epigenetic modifications and the intriguing possibility of their association with heritable behavioral phenotypes. The molecular basis of heritability of phenotypes arising from environment-induced epigenetic modifications is not well defined yet. However, phenomenological evidence in favor of it is accumulating rapidly. The resurgence of interest has led to focus on epigenetic...
Source: Biology of Reproduction - June 14, 2021 Category: Reproduction Medicine Authors: Deirdre M McCarthy Pradeep G Bhide Source Type: research

Heritable consequences of paternal nicotine exposure: From phenomena to mechanisms
Biol Reprod. 2021 Jun 14:ioab116. doi: 10.1093/biolre/ioab116. Online ahead of print.ABSTRACTOur understanding of the interactions between genetic and environmental factors in shaping behavioral phenotypes has expanded to include environment-induced epigenetic modifications and the intriguing possibility of their association with heritable behavioral phenotypes. The molecular basis of heritability of phenotypes arising from environment-induced epigenetic modifications is not well defined yet. However, phenomenological evidence in favor of it is accumulating rapidly. The resurgence of interest has led to focus on epigenetic...
Source: Biology of Reproduction - June 14, 2021 Category: Reproduction Medicine Authors: Deirdre M McCarthy Pradeep G Bhide Source Type: research

Germ cells of the mammalian female: A limited or renewable resource?
We describe and compare these conflicting studies with the aim of understanding how they came to opposing conclusions. We first consider studies that, by examining marker-gene expression, the fate of genetically marked cells, and consequences of depleting the oocyte population, addressed whether ovaries of post-natal females contain oogonial stem cells (OSC) that give rise to new oocytes. We next discuss whether ovaries contain cells that, even if inactive under physiological conditions, nonetheless possess OSC properties that can be revealed through cell-culture. We then examine studies of whether cells harvested after lo...
Source: Biology of Reproduction - June 11, 2021 Category: Reproduction Medicine Authors: Mathilde Hainaut Hugh J Clarke Source Type: research

Germ cells of the mammalian female: A limited or renewable resource?
We describe and compare these conflicting studies with the aim of understanding how they came to opposing conclusions. We first consider studies that, by examining marker-gene expression, the fate of genetically marked cells, and consequences of depleting the oocyte population, addressed whether ovaries of post-natal females contain oogonial stem cells (OSC) that give rise to new oocytes. We next discuss whether ovaries contain cells that, even if inactive under physiological conditions, nonetheless possess OSC properties that can be revealed through cell-culture. We then examine studies of whether cells harvested after lo...
Source: Biology of Reproduction - June 11, 2021 Category: Reproduction Medicine Authors: Mathilde Hainaut Hugh J Clarke Source Type: research

Germ cells of the mammalian female: A limited or renewable resource?
We describe and compare these conflicting studies with the aim of understanding how they came to opposing conclusions. We first consider studies that, by examining marker-gene expression, the fate of genetically marked cells, and consequences of depleting the oocyte population, addressed whether ovaries of post-natal females contain oogonial stem cells (OSC) that give rise to new oocytes. We next discuss whether ovaries contain cells that, even if inactive under physiological conditions, nonetheless possess OSC properties that can be revealed through cell-culture. We then examine studies of whether cells harvested after lo...
Source: Biology of Reproduction - June 11, 2021 Category: Reproduction Medicine Authors: Mathilde Hainaut Hugh J Clarke Source Type: research

Self-renewal and differentiation of rat Epididymal basal cells using a novel in vitro organoid model
The objectives of this study were to assess the ability of single basal cells to develop organoids and to assess their ability to self-renew and differentiate in vitro. We isolated basal cells from the rat epididymis and established 3-dimensional cell cultures from the basal and non-basal cell fractions. Organoids were formed by single adult epididymal basal cells. Organoids were dissociated into single basal cells which were able to reform new organoids, and were maintained over 10 generations. Long-term culture of organoids revealed that these cells could differentiated into cells expressing the principal cell markers aq...
Source: Biology of Reproduction - June 9, 2021 Category: Reproduction Medicine Authors: Laurie Pinel Daniel G Cyr Source Type: research

A high level of KLF12 causes folic acid-resistant neural tube defects by activating the Shh signalling pathway in mice
Biol Reprod. 2021 Jun 8:ioab111. doi: 10.1093/biolre/ioab111. Online ahead of print.ABSTRACTAlthough adequate periconceptional folic acid (FA) supplementation has reduced the occurrence of pregnancies affected by neural tube defects (NTDs), the mechanisms underlying FA-resistant NTDs are poorly understood, and thus NTDs still remain a global public health concern. A high level of Krüppel-like factor 12 (KLF12) exerts deleterious effects on heath in most cases, but evidence for its roles in development has not been published. We observed KLF12-overexpressing mice showed disturbed neural tube development. KLF12-overexpr...
Source: Biology of Reproduction - June 9, 2021 Category: Reproduction Medicine Authors: Yang Liu Qiong Yuan Zhilong Wang Lijun Ding Na Kong Jingyu Liu Yali Hu Yang Zhang Chaojun Li Guijun Yan Yue Jiang Haixiang Sun Source Type: research

Temporal and spatial expression of adrenomedullin and its receptors in the porcine uterus and peri-implantation conceptuses
This study determined abundances of ADM in uterine luminal fluid, and the patterns of expression of ADM and its receptor components (CALCRL, RAMP2, RAMP3, and ACKR3) in uteri from cyclic and pregnant gilts, as well as conceptuses (embryonic/fetus and its extra-embryonic membranes) during the peri-implantation period of pregnancy. Total recoverable ADM was greater in the uterine fluid of pregnant compared with cyclic gilts between Days 10 and 16 post-estrus, and was from uterine luminal epithelial (LE) and conceptus trophectoderm (Tr) cells. Uterine expression of CALCRL, RAMP2, and ACKR3 were affected by day (P
Source: Biology of Reproduction - June 9, 2021 Category: Reproduction Medicine Authors: Sudikshya Paudel Bangmin Liu Magdalina J Cummings Kelsey E Quinn Fuller W Bazer Kathleen M Caron Xiaoqiu Wang Source Type: research

Loss of Basigin expression in uterine cells leads to subfertility in female mice
Biol Reprod. 2021 Jun 9:ioab109. doi: 10.1093/biolre/ioab109. Online ahead of print.ABSTRACTBasigin (BSG) is a transmembrane glycoprotein involved in cell proliferation, angiogenesis and tissue remodeling. BSG has been shown to be essential for male and female reproduction although little is known about its role in normal uterine function. To study the potential function of BSG in the female reproductive tract, we generated mice with conditional knockout of Bsg in uterine cells using progesterone receptor-Cre and hypothesized that BSG is required for normal pregnancy in mice. Fertility study data showed that the conditiona...
Source: Biology of Reproduction - June 9, 2021 Category: Reproduction Medicine Authors: Kailiang Li Quanxi Li Shah Tauseef Bashir Brent M Bany Romana A Nowak Source Type: research

Self-renewal and differentiation of rat Epididymal basal cells using a novel in vitro organoid model
The objectives of this study were to assess the ability of single basal cells to develop organoids and to assess their ability to self-renew and differentiate in vitro. We isolated basal cells from the rat epididymis and established 3-dimensional cell cultures from the basal and non-basal cell fractions. Organoids were formed by single adult epididymal basal cells. Organoids were dissociated into single basal cells which were able to reform new organoids, and were maintained over 10 generations. Long-term culture of organoids revealed that these cells could differentiated into cells expressing the principal cell markers aq...
Source: Biology of Reproduction - June 9, 2021 Category: Reproduction Medicine Authors: Laurie Pinel Daniel G Cyr Source Type: research

A high level of KLF12 causes folic acid-resistant neural tube defects by activating the Shh signalling pathway in mice
Biol Reprod. 2021 Jun 8:ioab111. doi: 10.1093/biolre/ioab111. Online ahead of print.ABSTRACTAlthough adequate periconceptional folic acid (FA) supplementation has reduced the occurrence of pregnancies affected by neural tube defects (NTDs), the mechanisms underlying FA-resistant NTDs are poorly understood, and thus NTDs still remain a global public health concern. A high level of Krüppel-like factor 12 (KLF12) exerts deleterious effects on heath in most cases, but evidence for its roles in development has not been published. We observed KLF12-overexpressing mice showed disturbed neural tube development. KLF12-overexpr...
Source: Biology of Reproduction - June 9, 2021 Category: Reproduction Medicine Authors: Yang Liu Qiong Yuan Zhilong Wang Lijun Ding Na Kong Jingyu Liu Yali Hu Yang Zhang Chaojun Li Guijun Yan Yue Jiang Haixiang Sun Source Type: research

Temporal and spatial expression of adrenomedullin and its receptors in the porcine uterus and peri-implantation conceptuses
This study determined abundances of ADM in uterine luminal fluid, and the patterns of expression of ADM and its receptor components (CALCRL, RAMP2, RAMP3, and ACKR3) in uteri from cyclic and pregnant gilts, as well as conceptuses (embryonic/fetus and its extra-embryonic membranes) during the peri-implantation period of pregnancy. Total recoverable ADM was greater in the uterine fluid of pregnant compared with cyclic gilts between Days 10 and 16 post-estrus, and was from uterine luminal epithelial (LE) and conceptus trophectoderm (Tr) cells. Uterine expression of CALCRL, RAMP2, and ACKR3 were affected by day (P
Source: Biology of Reproduction - June 9, 2021 Category: Reproduction Medicine Authors: Sudikshya Paudel Bangmin Liu Magdalina J Cummings Kelsey E Quinn Fuller W Bazer Kathleen M Caron Xiaoqiu Wang Source Type: research

Loss of Basigin expression in uterine cells leads to subfertility in female mice
Biol Reprod. 2021 Jun 9:ioab109. doi: 10.1093/biolre/ioab109. Online ahead of print.ABSTRACTBasigin (BSG) is a transmembrane glycoprotein involved in cell proliferation, angiogenesis and tissue remodeling. BSG has been shown to be essential for male and female reproduction although little is known about its role in normal uterine function. To study the potential function of BSG in the female reproductive tract, we generated mice with conditional knockout of Bsg in uterine cells using progesterone receptor-Cre and hypothesized that BSG is required for normal pregnancy in mice. Fertility study data showed that the conditiona...
Source: Biology of Reproduction - June 9, 2021 Category: Reproduction Medicine Authors: Kailiang Li Quanxi Li Shah Tauseef Bashir Brent M Bany Romana A Nowak Source Type: research

Self-renewal and differentiation of rat Epididymal basal cells using a novel in vitro organoid model
The objectives of this study were to assess the ability of single basal cells to develop organoids and to assess their ability to self-renew and differentiate in vitro. We isolated basal cells from the rat epididymis and established 3-dimensional cell cultures from the basal and non-basal cell fractions. Organoids were formed by single adult epididymal basal cells. Organoids were dissociated into single basal cells which were able to reform new organoids, and were maintained over 10 generations. Long-term culture of organoids revealed that these cells could differentiated into cells expressing the principal cell markers aq...
Source: Biology of Reproduction - June 9, 2021 Category: Reproduction Medicine Authors: Laurie Pinel Daniel G Cyr Source Type: research

A high level of KLF12 causes folic acid-resistant neural tube defects by activating the Shh signalling pathway in mice
Biol Reprod. 2021 Jun 8:ioab111. doi: 10.1093/biolre/ioab111. Online ahead of print.ABSTRACTAlthough adequate periconceptional folic acid (FA) supplementation has reduced the occurrence of pregnancies affected by neural tube defects (NTDs), the mechanisms underlying FA-resistant NTDs are poorly understood, and thus NTDs still remain a global public health concern. A high level of Krüppel-like factor 12 (KLF12) exerts deleterious effects on heath in most cases, but evidence for its roles in development has not been published. We observed KLF12-overexpressing mice showed disturbed neural tube development. KLF12-overexpr...
Source: Biology of Reproduction - June 9, 2021 Category: Reproduction Medicine Authors: Yang Liu Qiong Yuan Zhilong Wang Lijun Ding Na Kong Jingyu Liu Yali Hu Yang Zhang Chaojun Li Guijun Yan Yue Jiang Haixiang Sun Source Type: research

Temporal and spatial expression of adrenomedullin and its receptors in the porcine uterus and peri-implantation conceptuses
This study determined abundances of ADM in uterine luminal fluid, and the patterns of expression of ADM and its receptor components (CALCRL, RAMP2, RAMP3, and ACKR3) in uteri from cyclic and pregnant gilts, as well as conceptuses (embryonic/fetus and its extra-embryonic membranes) during the peri-implantation period of pregnancy. Total recoverable ADM was greater in the uterine fluid of pregnant compared with cyclic gilts between Days 10 and 16 post-estrus, and was from uterine luminal epithelial (LE) and conceptus trophectoderm (Tr) cells. Uterine expression of CALCRL, RAMP2, and ACKR3 were affected by day (P
Source: Biology of Reproduction - June 9, 2021 Category: Reproduction Medicine Authors: Sudikshya Paudel Bangmin Liu Magdalina J Cummings Kelsey E Quinn Fuller W Bazer Kathleen M Caron Xiaoqiu Wang Source Type: research

Loss of Basigin expression in uterine cells leads to subfertility in female mice
Biol Reprod. 2021 Jun 9:ioab109. doi: 10.1093/biolre/ioab109. Online ahead of print.ABSTRACTBasigin (BSG) is a transmembrane glycoprotein involved in cell proliferation, angiogenesis and tissue remodeling. BSG has been shown to be essential for male and female reproduction although little is known about its role in normal uterine function. To study the potential function of BSG in the female reproductive tract, we generated mice with conditional knockout of Bsg in uterine cells using progesterone receptor-Cre and hypothesized that BSG is required for normal pregnancy in mice. Fertility study data showed that the conditiona...
Source: Biology of Reproduction - June 9, 2021 Category: Reproduction Medicine Authors: Kailiang Li Quanxi Li Shah Tauseef Bashir Brent M Bany Romana A Nowak Source Type: research

Aromatase and the three little paralogs
Biol Reprod. 2021 Jun 7:ioab112. doi: 10.1093/biolre/ioab112. Online ahead of print.ABSTRACTThe rapid progress of scientific knowledge is continuously built on established earlier findings. Integrating previous observations with more recent data can be challenging and occasionally well-established data is unfortunately overlooked. This may lead to unnecessary redundancy in research and, in the worst-case scenario, to misinterpretation of data. In the present contribution we would like to draw attention to such a circumstance, which has crept into several recent papers on expression and functional relevance of the porcine C...
Source: Biology of Reproduction - June 7, 2021 Category: Reproduction Medicine Authors: Jens Vanselow Alan J Conley Trish Berger Source Type: research

Aging and oxidative stress alter DNA repair mechanisms in male germ cells of superoxide dismutase-1 null mice
In this study, we evaluated the effects of aging and removing SOD1 (in male germ cells of SOD1-null (Sod1-/-) mice) in order to determine the potential mechanism(s) of DNA damage in these cells. Immunohistochemical analysis showed an increase in lipid peroxidation and DNA damage in the germ cells of aged wild-type (WT) and Sod1-/- mice of all age. Immunostaining of OGG1, a marker of base excision repair (BER), increased in aged WT and young Sod1-/- mice. In contrast, immunostaining intensity of LIGIV and RAD51, markers of non-homologous end-joining (NHEJ) and homologous recombination (HR), respectively, decreased in aged a...
Source: Biology of Reproduction - June 7, 2021 Category: Reproduction Medicine Authors: Paulina Nguyen-Powanda Bernard Robaire Source Type: research

Dynamics of small non-coding RNAs in bovine scNT embryos through the maternal-to-embryonic transition
The objective of this study was to examine dynamic changes in relative abundance of sncRNAs during the maternal-to embryonic transition (MET) in bovine embryos produced by scNT as compared to IVF by using RNA sequencing. When comparing populations of miRNA in scNT versus IVF embryos, only miR-2340, miR-345, and miR34a were differentially expressed in morulae, though many more miRNAs were differentially expressed when comparing across developmental stages. Also of interest, distinct populations of piwi-interacting like RNAs (pilRNAs) were identified in bovine embryos prior to and during embryonic genome activation (EGA) as ...
Source: Biology of Reproduction - June 4, 2021 Category: Reproduction Medicine Authors: Jocelyn M Cuthbert Stewart J Russell Irina A Polejaeva Qinggang Meng Kenneth L White Abby D Benninghoff Source Type: research

Iodoacetic acid affects Estrous Cyclicity, ovarian gene expression, and hormone levels in mice
In conclusion, IAA exposure alters estrous cyclicity, ovarian gene expression, and estradiol levels in mice.PMID:34086879 | DOI:10.1093/biolre/ioab108 (Source: Biology of Reproduction)
Source: Biology of Reproduction - June 4, 2021 Category: Reproduction Medicine Authors: Andressa Gonsioroski Daryl D Meling Liying Gao Michael J Plewa Jodi A Flaws Source Type: research

Dynamics of small non-coding RNAs in bovine scNT embryos through the maternal-to-embryonic transition
The objective of this study was to examine dynamic changes in relative abundance of sncRNAs during the maternal-to embryonic transition (MET) in bovine embryos produced by scNT as compared to IVF by using RNA sequencing. When comparing populations of miRNA in scNT versus IVF embryos, only miR-2340, miR-345, and miR34a were differentially expressed in morulae, though many more miRNAs were differentially expressed when comparing across developmental stages. Also of interest, distinct populations of piwi-interacting like RNAs (pilRNAs) were identified in bovine embryos prior to and during embryonic genome activation (EGA) as ...
Source: Biology of Reproduction - June 4, 2021 Category: Reproduction Medicine Authors: Jocelyn M Cuthbert Stewart J Russell Irina A Polejaeva Qinggang Meng Kenneth L White Abby D Benninghoff Source Type: research

Iodoacetic acid affects Estrous Cyclicity, ovarian gene expression, and hormone levels in mice
In conclusion, IAA exposure alters estrous cyclicity, ovarian gene expression, and estradiol levels in mice.PMID:34086879 | DOI:10.1093/biolre/ioab108 (Source: Biology of Reproduction)
Source: Biology of Reproduction - June 4, 2021 Category: Reproduction Medicine Authors: Andressa Gonsioroski Daryl D Meling Liying Gao Michael J Plewa Jodi A Flaws Source Type: research

Dynamics of small non-coding RNAs in bovine scNT embryos through the maternal-to-embryonic transition
The objective of this study was to examine dynamic changes in relative abundance of sncRNAs during the maternal-to embryonic transition (MET) in bovine embryos produced by scNT as compared to IVF by using RNA sequencing. When comparing populations of miRNA in scNT versus IVF embryos, only miR-2340, miR-345, and miR34a were differentially expressed in morulae, though many more miRNAs were differentially expressed when comparing across developmental stages. Also of interest, distinct populations of piwi-interacting like RNAs (pilRNAs) were identified in bovine embryos prior to and during embryonic genome activation (EGA) as ...
Source: Biology of Reproduction - June 4, 2021 Category: Reproduction Medicine Authors: Jocelyn M Cuthbert Stewart J Russell Irina A Polejaeva Qinggang Meng Kenneth L White Abby D Benninghoff Source Type: research

Iodoacetic acid affects Estrous Cyclicity, ovarian gene expression, and hormone levels in mice
In conclusion, IAA exposure alters estrous cyclicity, ovarian gene expression, and estradiol levels in mice.PMID:34086879 | DOI:10.1093/biolre/ioab108 (Source: Biology of Reproduction)
Source: Biology of Reproduction - June 4, 2021 Category: Reproduction Medicine Authors: Andressa Gonsioroski Daryl D Meling Liying Gao Michael J Plewa Jodi A Flaws Source Type: research

NAD+ deficiency and mitochondrial dysfunction in granulosa cells of women with polycystic ovary syndrome ‡
Biol Reprod. 2021 May 29:ioab078. doi: 10.1093/biolre/ioab078. Online ahead of print.ABSTRACTPolycystic ovary syndrome (PCOS) is a prevalent heterogeneous endocrine disorder characterized by ovulation dysfunction, androgen excess, ovarian polycystic changes, insulin resistance, and infertility. Although underlying mechanisms for PCOS are still unknown, inflammation and mitochondrial dysfunction in granulosa cells (GCs) of PCOS patients have been reported. Here, we found that Nicotinamide Adenine Dinucleotide (NAD+) levels in GCs of PCOS patients was significantly decreased when compared with controls. Also, we found that h...
Source: Biology of Reproduction - May 31, 2021 Category: Reproduction Medicine Authors: Yujiao Wang Qingling Yang Huan Wang Jing Zhu Luping Cong Hui Li Yingpu Sun Source Type: research

Sperm migration, selection, survival and fertilizing ability in the mammalian oviduct
Biol Reprod. 2021 May 31:ioab105. doi: 10.1093/biolre/ioab105. Online ahead of print.ABSTRACTIn vitro fertilization (IVF) gives rise to embryos in a number of mammalian species and is currently widely used for assisted reproduction in humans and for genetic purposes in cattle. However, the rate of polyspermy is generally higher in vitro than in vivo and IVF remains ineffective in some domestic species like pigs and horses, highlighting the importance of the female reproductive tract for gamete quality and fertilization. In this review, the way the female environment modulates sperm selective migration, survival and acquisi...
Source: Biology of Reproduction - May 31, 2021 Category: Reproduction Medicine Authors: Coline Mah é Aleksandra Maria Zlotkowska Karine Reynaud Guillaume Tsikis Pascal Mermillod Xavier Druart Jennifer Schoen Marie Saint-Dizier Source Type: research

Differential localization of serotoninergic system elements in human amniotic epithelial cells †
Biol Reprod. 2021 May 31:ioab106. doi: 10.1093/biolre/ioab106. Online ahead of print.ABSTRACTSerotonin or 5-hydroxytryptamine (5-HT) is a biogenic amine involved in regulating several functions, including development. However, its impact on human embryo development has been poorly studied. The present work investigated the expression and distribution of the main components of the serotoninergic system in human amniotic tissue and in human amniotic epithelial cells (hAEC) in vitro, as an alternative model of early human embryo development. Amniotic membranes from full-term healthy pregnancies were used. Human amnion tissue ...
Source: Biology of Reproduction - May 31, 2021 Category: Reproduction Medicine Authors: Jessica Romero-Reyes Edgar Ricardo V ázquez-Martínez Daniel Bahena-Alvarez Jessica L ópez-Jiménez Anayansi Molina-Hern ández Ignacio Camacho-Arroyo N éstor Fabián Díaz Source Type: research

Sertoli cell replacement in explanted mouse testis tissue supporting host spermatogenesis
In this study, we developed an in vitro Sertoli cell replacement method by combining an organ culture technique and a toxin receptor-mediated cell knockout (Treck) system. We used Amh- diphtheria toxin receptor (DTR) transgenic mice, whose Sertoli cells specifically express human DTR, which renders them sensitive to diphtheria toxin (DT). An immature Amh-DTR testis was transplanted with donor testis cells followed by culturing in a medium containing DT. This procedure successfully replaced the original Sertoli cells with the transplanted Sertoli cells, and spermatogenesis originating from resident germ cells was confirmed....
Source: Biology of Reproduction - May 31, 2021 Category: Reproduction Medicine Authors: Kazusa Higuch Takafumi Matsumura Haruhiko Akiyama Yoshiakira Kanai Takehiko Ogawa Takuya Sato Source Type: research

NAD+ deficiency and mitochondrial dysfunction in granulosa cells of women with polycystic ovary syndrome ‡
Biol Reprod. 2021 May 29:ioab078. doi: 10.1093/biolre/ioab078. Online ahead of print.ABSTRACTPolycystic ovary syndrome (PCOS) is a prevalent heterogeneous endocrine disorder characterized by ovulation dysfunction, androgen excess, ovarian polycystic changes, insulin resistance, and infertility. Although underlying mechanisms for PCOS are still unknown, inflammation and mitochondrial dysfunction in granulosa cells (GCs) of PCOS patients have been reported. Here, we found that Nicotinamide Adenine Dinucleotide (NAD+) levels in GCs of PCOS patients was significantly decreased when compared with controls. Also, we found that h...
Source: Biology of Reproduction - May 31, 2021 Category: Reproduction Medicine Authors: Yujiao Wang Qingling Yang Huan Wang Jing Zhu Luping Cong Hui Li Yingpu Sun Source Type: research

Sperm migration, selection, survival and fertilizing ability in the mammalian oviduct
Biol Reprod. 2021 May 31:ioab105. doi: 10.1093/biolre/ioab105. Online ahead of print.ABSTRACTIn vitro fertilization (IVF) gives rise to embryos in a number of mammalian species and is currently widely used for assisted reproduction in humans and for genetic purposes in cattle. However, the rate of polyspermy is generally higher in vitro than in vivo and IVF remains ineffective in some domestic species like pigs and horses, highlighting the importance of the female reproductive tract for gamete quality and fertilization. In this review, the way the female environment modulates sperm selective migration, survival and acquisi...
Source: Biology of Reproduction - May 31, 2021 Category: Reproduction Medicine Authors: Coline Mah é Aleksandra Maria Zlotkowska Karine Reynaud Guillaume Tsikis Pascal Mermillod Xavier Druart Jennifer Schoen Marie Saint-Dizier Source Type: research

Differential localization of serotoninergic system elements in human amniotic epithelial cells †
Biol Reprod. 2021 May 31:ioab106. doi: 10.1093/biolre/ioab106. Online ahead of print.ABSTRACTSerotonin or 5-hydroxytryptamine (5-HT) is a biogenic amine involved in regulating several functions, including development. However, its impact on human embryo development has been poorly studied. The present work investigated the expression and distribution of the main components of the serotoninergic system in human amniotic tissue and in human amniotic epithelial cells (hAEC) in vitro, as an alternative model of early human embryo development. Amniotic membranes from full-term healthy pregnancies were used. Human amnion tissue ...
Source: Biology of Reproduction - May 31, 2021 Category: Reproduction Medicine Authors: Jessica Romero-Reyes Edgar Ricardo V ázquez-Martínez Daniel Bahena-Alvarez Jessica L ópez-Jiménez Anayansi Molina-Hern ández Ignacio Camacho-Arroyo N éstor Fabián Díaz Source Type: research

Sertoli cell replacement in explanted mouse testis tissue supporting host spermatogenesis
In this study, we developed an in vitro Sertoli cell replacement method by combining an organ culture technique and a toxin receptor-mediated cell knockout (Treck) system. We used Amh- diphtheria toxin receptor (DTR) transgenic mice, whose Sertoli cells specifically express human DTR, which renders them sensitive to diphtheria toxin (DT). An immature Amh-DTR testis was transplanted with donor testis cells followed by culturing in a medium containing DT. This procedure successfully replaced the original Sertoli cells with the transplanted Sertoli cells, and spermatogenesis originating from resident germ cells was confirmed....
Source: Biology of Reproduction - May 31, 2021 Category: Reproduction Medicine Authors: Kazusa Higuch Takafumi Matsumura Haruhiko Akiyama Yoshiakira Kanai Takehiko Ogawa Takuya Sato Source Type: research

NAD+ deficiency and mitochondrial dysfunction in granulosa cells of women with polycystic ovary syndrome ‡
Biol Reprod. 2021 May 29:ioab078. doi: 10.1093/biolre/ioab078. Online ahead of print.ABSTRACTPolycystic ovary syndrome (PCOS) is a prevalent heterogeneous endocrine disorder characterized by ovulation dysfunction, androgen excess, ovarian polycystic changes, insulin resistance, and infertility. Although underlying mechanisms for PCOS are still unknown, inflammation and mitochondrial dysfunction in granulosa cells (GCs) of PCOS patients have been reported. Here, we found that Nicotinamide Adenine Dinucleotide (NAD+) levels in GCs of PCOS patients was significantly decreased when compared with controls. Also, we found that h...
Source: Biology of Reproduction - May 31, 2021 Category: Reproduction Medicine Authors: Yujiao Wang Qingling Yang Huan Wang Jing Zhu Luping Cong Hui Li Yingpu Sun Source Type: research

Sperm migration, selection, survival and fertilizing ability in the mammalian oviduct
Biol Reprod. 2021 May 31:ioab105. doi: 10.1093/biolre/ioab105. Online ahead of print.ABSTRACTIn vitro fertilization (IVF) gives rise to embryos in a number of mammalian species and is currently widely used for assisted reproduction in humans and for genetic purposes in cattle. However, the rate of polyspermy is generally higher in vitro than in vivo and IVF remains ineffective in some domestic species like pigs and horses, highlighting the importance of the female reproductive tract for gamete quality and fertilization. In this review, the way the female environment modulates sperm selective migration, survival and acquisi...
Source: Biology of Reproduction - May 31, 2021 Category: Reproduction Medicine Authors: Coline Mah é Aleksandra Maria Zlotkowska Karine Reynaud Guillaume Tsikis Pascal Mermillod Xavier Druart Jennifer Schoen Marie Saint-Dizier Source Type: research

Differential localization of serotoninergic system elements in human amniotic epithelial cells †
Biol Reprod. 2021 May 31:ioab106. doi: 10.1093/biolre/ioab106. Online ahead of print.ABSTRACTSerotonin or 5-hydroxytryptamine (5-HT) is a biogenic amine involved in regulating several functions, including development. However, its impact on human embryo development has been poorly studied. The present work investigated the expression and distribution of the main components of the serotoninergic system in human amniotic tissue and in human amniotic epithelial cells (hAEC) in vitro, as an alternative model of early human embryo development. Amniotic membranes from full-term healthy pregnancies were used. Human amnion tissue ...
Source: Biology of Reproduction - May 31, 2021 Category: Reproduction Medicine Authors: Jessica Romero-Reyes Edgar Ricardo V ázquez-Martínez Daniel Bahena-Alvarez Jessica L ópez-Jiménez Anayansi Molina-Hern ández Ignacio Camacho-Arroyo N éstor Fabián Díaz Source Type: research

Sertoli cell replacement in explanted mouse testis tissue supporting host spermatogenesis
In this study, we developed an in vitro Sertoli cell replacement method by combining an organ culture technique and a toxin receptor-mediated cell knockout (Treck) system. We used Amh- diphtheria toxin receptor (DTR) transgenic mice, whose Sertoli cells specifically express human DTR, which renders them sensitive to diphtheria toxin (DT). An immature Amh-DTR testis was transplanted with donor testis cells followed by culturing in a medium containing DT. This procedure successfully replaced the original Sertoli cells with the transplanted Sertoli cells, and spermatogenesis originating from resident germ cells was confirmed....
Source: Biology of Reproduction - May 31, 2021 Category: Reproduction Medicine Authors: Kazusa Higuch Takafumi Matsumura Haruhiko Akiyama Yoshiakira Kanai Takehiko Ogawa Takuya Sato Source Type: research

NAD+ deficiency and mitochondrial dysfunction in granulosa cells of women with polycystic ovary syndrome ‡
Biol Reprod. 2021 May 29:ioab078. doi: 10.1093/biolre/ioab078. Online ahead of print.ABSTRACTPolycystic ovary syndrome (PCOS) is a prevalent heterogeneous endocrine disorder characterized by ovulation dysfunction, androgen excess, ovarian polycystic changes, insulin resistance, and infertility. Although underlying mechanisms for PCOS are still unknown, inflammation and mitochondrial dysfunction in granulosa cells (GCs) of PCOS patients have been reported. Here, we found that Nicotinamide Adenine Dinucleotide (NAD+) levels in GCs of PCOS patients was significantly decreased when compared with controls. Also, we found that h...
Source: Biology of Reproduction - May 31, 2021 Category: Reproduction Medicine Authors: Yujiao Wang Qingling Yang Huan Wang Jing Zhu Luping Cong Hui Li Yingpu Sun Source Type: research