IGFBP1a is a nutrient deficient response factor that can inhibit fish reproduction through the hypothalamus-pituitary-ovary axis †
Biol Reprod. 2024 Feb 19:ioae009. doi: 10.1093/biolre/ioae009. Online ahead of print.ABSTRACTReproduction is a high energy consuming process, so long-term malnutrition can significantly inhibit gonadal development. However, little is known about the molecular mechanism by which fasting inhibits reproduction. Our present study found that fasting could dramatically induce insulin-like growth factor binding protein 1 (IGFBP1) expression in the liver, hypothalamus, pituitary and ovaries of grass carp. In addition, IGFBP1a in the hypothalamus-pituitary-gonad axis could inhibit the development of gonads. These results indicated ...
Source: Biology of Reproduction - February 20, 2024 Category: Reproduction Medicine Authors: Wei Li Hangyu Li Qiongyao Hu Linlin Wang Zhan Yin Guangfu Hu Source Type: research
Inefficient Sox9 upregulation and absence of Rspo1 repression lead to sex reversal in the B6.XY TIR mouse gonad
Biol Reprod. 2024 Feb 20:ioae018. doi: 10.1093/biolre/ioae018. Online ahead of print.ABSTRACTSry on the Y-chromosome upregulates Sox9, which in turn upregulates a set of genes such as Fgf9 to initiate testicular differentiation in the XY gonad. In the absence of Sry expression, genes such as Rspo1, Foxl2, and Runx1 support ovarian differentiation in the XX gonad. These two pathways antagonize each other to ensure the development of only one gonadal sex in normal development. In the B6.YTIR mouse, carrying the YTIR-chromosome on the B6 genetic background, Sry is expressed in a comparable manner with that in the B6.XY mouse,...
Source: Biology of Reproduction - February 20, 2024 Category: Reproduction Medicine Authors: Jiangqin Cao Fatima El Mansouri Sofia Reynoso Zongping Liu Jiaqiao Zhu Teruko Taketo Source Type: research
IGFBP1a is a nutrient deficient response factor that can inhibit fish reproduction through the hypothalamus-pituitary-ovary axis †
Biol Reprod. 2024 Feb 19:ioae009. doi: 10.1093/biolre/ioae009. Online ahead of print.ABSTRACTReproduction is a high energy consuming process, so long-term malnutrition can significantly inhibit gonadal development. However, little is known about the molecular mechanism by which fasting inhibits reproduction. Our present study found that fasting could dramatically induce insulin-like growth factor binding protein 1 (IGFBP1) expression in the liver, hypothalamus, pituitary and ovaries of grass carp. In addition, IGFBP1a in the hypothalamus-pituitary-gonad axis could inhibit the development of gonads. These results indicated ...
Source: Biology of Reproduction - February 20, 2024 Category: Reproduction Medicine Authors: Wei Li Hangyu Li Qiongyao Hu Linlin Wang Zhan Yin Guangfu Hu Source Type: research
Inefficient Sox9 upregulation and absence of Rspo1 repression lead to sex reversal in the B6.XY TIR mouse gonad
Biol Reprod. 2024 Feb 20:ioae018. doi: 10.1093/biolre/ioae018. Online ahead of print.ABSTRACTSry on the Y-chromosome upregulates Sox9, which in turn upregulates a set of genes such as Fgf9 to initiate testicular differentiation in the XY gonad. In the absence of Sry expression, genes such as Rspo1, Foxl2, and Runx1 support ovarian differentiation in the XX gonad. These two pathways antagonize each other to ensure the development of only one gonadal sex in normal development. In the B6.YTIR mouse, carrying the YTIR-chromosome on the B6 genetic background, Sry is expressed in a comparable manner with that in the B6.XY mouse,...
Source: Biology of Reproduction - February 20, 2024 Category: Reproduction Medicine Authors: Jiangqin Cao Fatima El Mansouri Sofia Reynoso Zongping Liu Jiaqiao Zhu Teruko Taketo Source Type: research
Membrane Inflammasome activation by Choriodecidual Ureaplasma parvum infection without intra-amniotic infection in an NHP model
Biol Reprod. 2024 Feb 9:ioae027. doi: 10.1093/biolre/ioae027. Online ahead of print.ABSTRACTIntrauterine infection is a significant cause of preterm labor and neonatal morbidity and mortality. Ureaplasma parvum is the microorganism most commonly isolated from cases of preterm birth and preterm premature rupture of membranes (pPROM). However, the mechanisms during the early stages of ascending reproductive tract infection remain poorly understood. To examine inflammation in fetal (chorioamnionic) membranes in response to U. parvum infection, we utilized a non-human primate (NHP) model of choriodecidual infection. Eight chro...
Source: Biology of Reproduction - February 9, 2024 Category: Reproduction Medicine Authors: Sudeshna Tripathy Irina Burd Meredith A Kelleher Source Type: research
Membrane Inflammasome activation by Choriodecidual Ureaplasma parvum infection without intra-amniotic infection in an NHP model
Biol Reprod. 2024 Feb 9:ioae027. doi: 10.1093/biolre/ioae027. Online ahead of print.ABSTRACTIntrauterine infection is a significant cause of preterm labor and neonatal morbidity and mortality. Ureaplasma parvum is the microorganism most commonly isolated from cases of preterm birth and preterm premature rupture of membranes (pPROM). However, the mechanisms during the early stages of ascending reproductive tract infection remain poorly understood. To examine inflammation in fetal (chorioamnionic) membranes in response to U. parvum infection, we utilized a non-human primate (NHP) model of choriodecidual infection. Eight chro...
Source: Biology of Reproduction - February 9, 2024 Category: Reproduction Medicine Authors: Sudeshna Tripathy Irina Burd Meredith A Kelleher Source Type: research
Membrane Inflammasome activation by Choriodecidual Ureaplasma parvum infection without intra-amniotic infection in an NHP model
Biol Reprod. 2024 Feb 9:ioae027. doi: 10.1093/biolre/ioae027. Online ahead of print.ABSTRACTIntrauterine infection is a significant cause of preterm labor and neonatal morbidity and mortality. Ureaplasma parvum is the microorganism most commonly isolated from cases of preterm birth and preterm premature rupture of membranes (pPROM). However, the mechanisms during the early stages of ascending reproductive tract infection remain poorly understood. To examine inflammation in fetal (chorioamnionic) membranes in response to U. parvum infection, we utilized a non-human primate (NHP) model of choriodecidual infection. Eight chro...
Source: Biology of Reproduction - February 9, 2024 Category: Reproduction Medicine Authors: Sudeshna Tripathy Irina Burd Meredith A Kelleher Source Type: research
Membrane Inflammasome activation by Choriodecidual Ureaplasma parvum infection without intra-amniotic infection in an NHP model
Biol Reprod. 2024 Feb 9:ioae027. doi: 10.1093/biolre/ioae027. Online ahead of print.ABSTRACTIntrauterine infection is a significant cause of preterm labor and neonatal morbidity and mortality. Ureaplasma parvum is the microorganism most commonly isolated from cases of preterm birth and preterm premature rupture of membranes (pPROM). However, the mechanisms during the early stages of ascending reproductive tract infection remain poorly understood. To examine inflammation in fetal (chorioamnionic) membranes in response to U. parvum infection, we utilized a non-human primate (NHP) model of choriodecidual infection. Eight chro...
Source: Biology of Reproduction - February 9, 2024 Category: Reproduction Medicine Authors: Sudeshna Tripathy Irina Burd Meredith A Kelleher Source Type: research
Membrane Inflammasome activation by Choriodecidual Ureaplasma parvum infection without intra-amniotic infection in an NHP model
Biol Reprod. 2024 Feb 9:ioae027. doi: 10.1093/biolre/ioae027. Online ahead of print.ABSTRACTIntrauterine infection is a significant cause of preterm labor and neonatal morbidity and mortality. Ureaplasma parvum is the microorganism most commonly isolated from cases of preterm birth and preterm premature rupture of membranes (pPROM). However, the mechanisms during the early stages of ascending reproductive tract infection remain poorly understood. To examine inflammation in fetal (chorioamnionic) membranes in response to U. parvum infection, we utilized a non-human primate (NHP) model of choriodecidual infection. Eight chro...
Source: Biology of Reproduction - February 9, 2024 Category: Reproduction Medicine Authors: Sudeshna Tripathy Irina Burd Meredith A Kelleher Source Type: research
Establishment and comparison of human term placenta-derived trophoblast cells
In this study, we describe the development of a human-term placenta-derived cytotrophoblast immortalized cell line (hPTCCTB) derived from the basal plate. hPTCCTB cells are comparable to their primary cells of origin in terms of morphology, marker expression, and functional responses. We demonstrate that these can transform into syncytiotrophoblast and extravillous trophoblasts. We also compared the hPTCCTB cells to immortalized chorionic trophoblasts (hFM-CTC), trophoblasts of the chorionic plate, and BeWo cells, choriocarcinoma cell lines of conventional use. hPTCCTB and hFM-CTCs displayed more similarity to each other t...
Source: Biology of Reproduction - February 8, 2024 Category: Reproduction Medicine Authors: Manuel S Vidal Enkhtuya Radnaa Natasha Vora Kamil Khanipov Cristina Antich Marc Ferrer Rheanna Urrabaz-Garza Jeena E Jacob Ramkumar Menon Source Type: research
Bta-miR-301a targets ACVR1 to influence cleavage time and blastocyst formation rate of early embryos in cattle
In this study, bioinformatics, dual luciferase reporter assays, rescue experiments and gain- and loss-of-function experiments indicated that ACVR1 is the target gene of bta-miR-301a in early bovine embryos. By microinjecting bta-miR-301a mimic into embryos of parthenogenetic or somatic cell nuclear transfer, we observed that bta-miR-301a prolonged the first cleavage time of the embryos and increased the blastocyst formation rate. Thus, this study provides preliminary evidence that bta-miR-301a influences remodeling of the microfilament skeleton, prolongs the first cleavage time, and improves the developmental competence of...
Source: Biology of Reproduction - February 6, 2024 Category: Reproduction Medicine Authors: Heqiang Li Jing Gao Jingyi Wang Baobao Zhao Xiaonan Ma Bihan Zhang Changrong Lv Hailian Qiao Yongsheng Wang Suzhu Qing Source Type: research
Cyclophosphamide activates ferroptosis induced dysfunction of Leydig cells via SMAD2 pathway
In this study, The level of ferroptosis in the mouse model of testicular Leydig cell dysfunction induced by CP was significantly increased and further affected testosterone synthesis. The ferroptosis inhibitors ferrostatin-1and iron chelator deferoxamine can improve injury induced by CP. The results of immunohistochemistry showed that ferrostatin-1 and deferoxamine could improve the structural disorder of seminiferous tubules and the decrease of the number of Leydig cells in testicular tissue induced by CP. Immunofluorescence and western blot confirmed that ferrostatin-1and deferoxamine could improve the expression of key ...
Source: Biology of Reproduction - February 6, 2024 Category: Reproduction Medicine Authors: Senlin Liao Cun Wei Guanyang Wei Haoyu Liang Fan Peng Lei Zhao Ziguang Li Cundong Liu Qizhao Zhou Source Type: research
New uses for an old technique: live imaging on the slice organ culture to study reproductive processes
Biol Reprod. 2024 Feb 5:ioae023. doi: 10.1093/biolre/ioae023. Online ahead of print.ABSTRACTReproductive processes are dynamic and involve extensive morphological remodeling and cell-cell interactions. Live imaging of organs enhances our understanding of how biological processes occur in real time. Slice culture is a type of organ culture where thick slices are collected from an organ and cultured for several days. Slice culture is a useful and easy-to-implement technique for live imaging of reproductive events at cellular resolution. Here we describe a pipeline of live imaging on slice culture to visualize the process of ...
Source: Biology of Reproduction - February 5, 2024 Category: Reproduction Medicine Authors: Ciro Maurizio Amato Humphrey Hung-Chang Yao Source Type: research
An interview with Dr. Daniel Johnston
Biol Reprod. 2024 Feb 1:ioae021. doi: 10.1093/biolre/ioae021. Online ahead of print.NO ABSTRACTPMID:38302090 | DOI:10.1093/biolre/ioae021 (Source: Biology of Reproduction)
Source: Biology of Reproduction - February 1, 2024 Category: Reproduction Medicine Authors: T Rajendra Kumar Source Type: research
Foxp2 deficiency impairs reproduction by modulating the hypothalamic-pituitary-gonadal axis in zebrafish
Biol Reprod. 2024 Jan 30:ioae019. doi: 10.1093/biolre/ioae019. Online ahead of print.ABSTRACTFOXP2 was initially characterized as a transcription factor linked to speech and language disorders. Single-cell RNA sequencing (scRNA-seq) reveals that Foxp2 is enriched in the gonadotrope cluster of the pituitary gland and colocalized with the hormones LHB and FSHB in chickens and mice, implying that FOXP2 might be associated with reproduction in vertebrates. Herein, we investigated the roles of foxp2 in reproduction in a Foxp2-deficient zebrafish model. The results indicated that the loss of Foxp2 inhibits courtship behavior in ...
Source: Biology of Reproduction - January 30, 2024 Category: Reproduction Medicine Authors: Maya Wang Juanjuan Luo Lu Dai Meilan Feng Xiaoqian Cao Jiannan Zhang Yiping Wan Xiaojun Yang Yajun Wang Source Type: research
