Sestrin2 and Sestrin3 protect spermatogenesis against heat-induced meiotic defects
Biol Reprod. 2024 Mar 22:ioae042. doi: 10.1093/biolre/ioae042. Online ahead of print.ABSTRACTHeat stress (HS) induces testicular oxidative stress, impairs spermatogenesis, and increases the risk of male infertility. Recent studies have highlighted the antioxidative properties of the Sestrins family in reducing cellular oxidative damage. However, the role of Sestrins (Sestrin1, 2, and 3) in the testicular response to heat stress remains unclear. Here, we found that Sestrin 2 and 3 were highly expressed in the testis relative to Sestrin1. Then, the Sestrin2-/- and Sestrin3-/- mice were generated by CRISPR/Cas9 to investigate...
Source: Biology of Reproduction - March 22, 2024 Category: Reproduction Medicine Authors: Wenhui Chen Mengchen Wang Huan Wang Yuqing Jiang Jing Zhu Xinxin Zeng Huihui Xie Qingling Yang Yingpu Sun Source Type: research
Interleukin-6 supplementation improves bovine conceptus elongation and transcriptomic indicators of developmental competence
Biol Reprod. 2024 Mar 22:ioae045. doi: 10.1093/biolre/ioae045. Online ahead of print.ABSTRACTA high incidence of pregnancy failures occurs in cattle during the second week of pregnancy as blastocysts transition into an elongated conceptus. This work explored whether interleukin-6 (IL6) supplementation during in vitro embryo production would improve subsequent conceptus development. Bovine embryos were treated with 0 or 100 ng/mL recombinant bovine IL6 beginning on day 5 post-fertilization. At day 7.5 post-fertilization, blastocysts were transferred into estrus synchronized beef cows (n = 5 recipients/treatment, 10 embryos/...
Source: Biology of Reproduction - March 22, 2024 Category: Reproduction Medicine Authors: Savannah L Speckhart Mary A Oliver Jessica A Keane Nicholas W Dias Vitor R G Mercadante Fernando H Biase Alan D Ealy Source Type: research
Pig conceptuses utilize extracellular vesicles for IFNG-mediated paracrine communication with the endometrium
Biol Reprod. 2024 Mar 19:ioae044. doi: 10.1093/biolre/ioae044. Online ahead of print.ABSTRACTInterferon-gamma (IFNG) is a pro-inflammatory cytokine secreted by the porcine conceptus (embryo and extra-embryonic membranes) during the peri-implantation period of pregnancy. IFNG modifies the endometrial inflammatory immune response and is required for the implantation and survival of the conceptus. It is not known how IFNG from the conceptus trophectoderm is transported across the endometrial luminal epithelium (LE). In the present study, immunofluorescence analyses detected immunoreactive IFNG protein in both the trophectoder...
Source: Biology of Reproduction - March 19, 2024 Category: Reproduction Medicine Authors: Joe W Cain Heewon Seo Katie Bumgardner Carli Lefevre Robert C Burghardt Fuller W Bazer Gregory A Johnson Source Type: research
Interleukin-10: a novel metabolic inducer of macrophage differentiation and subsequently contributing to improved pregnancy outcomes of mice by orchestrating oxidative phosphorylation metabolism
In this study, we mainly investigate the metabolic changes involved in IL-10 generated macrophages from monocytes using in vitro models. We demonstrate that exposure of monocytes (either peripheral or THP-1) to IL-10 altered the phenotype and function of resultant macrophages that is linked with OXPHOS changes. IL-10 enhanced the mitochondrial complex I and III activity of THP-1 cell-differentiated macrophages and increased the mitochondrial membrane potential, intracellular adenosine triphosphate, and reactive oxygen species levels. OXPHOS blockage with oligomycin changed the cell morphology of IL-10-generated macrophages...
Source: Biology of Reproduction - March 19, 2024 Category: Reproduction Medicine Authors: Huan Wang Liling Wang Guangshun Gong Xinxiu Lin Jing Luo Chunyan Liu Gil Mor Aihua Liao Source Type: research
Metabolic pathways for glucose and fructose: I synthesis and metabolism of fructose by ovine conceptuses
This study characterized key enzymes involved in fructose synthesis and metabolism by ovine conceptuses throughout pregnancy. Day 17 conceptuses expressed mRNAs for the polyol pathway (SORD and AKR1B1) and glucose and fructose metabolism (HK1, HK2, G6PD, OGT, and FBP), but not those required for gluconeogenesis (G6Pase or PCK). Ovine placentomes also expressed mRNAs for SORD, AKR1B1, HK1, and OGT. Fructose can be metabolized via the ketohexokinase (KHK) pathway and isoforms, KHK-A and KHK-C, were expressed in ovine conceptuses from Day 16 of pregnancy and placentomes during pregnancy in a cell specific manner: KHK-A protei...
Source: Biology of Reproduction - March 19, 2024 Category: Reproduction Medicine Authors: Robyn M Moses Claire Stenhouse Katherine M Halloran Nirvay Sah Emily C Hoskins Shannon E Washburn Gregory A Johnson Guoyao Wu Fuller W Bazer Source Type: research
Pig conceptuses utilize extracellular vesicles for IFNG-mediated paracrine communication with the endometrium
Biol Reprod. 2024 Mar 19:ioae044. doi: 10.1093/biolre/ioae044. Online ahead of print.ABSTRACTInterferon-gamma (IFNG) is a pro-inflammatory cytokine secreted by the porcine conceptus (embryo and extra-embryonic membranes) during the peri-implantation period of pregnancy. IFNG modifies the endometrial inflammatory immune response and is required for the implantation and survival of the conceptus. It is not known how IFNG from the conceptus trophectoderm is transported across the endometrial luminal epithelium (LE). In the present study, immunofluorescence analyses detected immunoreactive IFNG protein in both the trophectoder...
Source: Biology of Reproduction - March 19, 2024 Category: Reproduction Medicine Authors: Joe W Cain Heewon Seo Katie Bumgardner Carli Lefevre Robert C Burghardt Fuller W Bazer Gregory A Johnson Source Type: research
Interleukin-10: a novel metabolic inducer of macrophage differentiation and subsequently contributing to improved pregnancy outcomes of mice by orchestrating oxidative phosphorylation metabolism
In this study, we mainly investigate the metabolic changes involved in IL-10 generated macrophages from monocytes using in vitro models. We demonstrate that exposure of monocytes (either peripheral or THP-1) to IL-10 altered the phenotype and function of resultant macrophages that is linked with OXPHOS changes. IL-10 enhanced the mitochondrial complex I and III activity of THP-1 cell-differentiated macrophages and increased the mitochondrial membrane potential, intracellular adenosine triphosphate, and reactive oxygen species levels. OXPHOS blockage with oligomycin changed the cell morphology of IL-10-generated macrophages...
Source: Biology of Reproduction - March 19, 2024 Category: Reproduction Medicine Authors: Huan Wang Liling Wang Guangshun Gong Xinxiu Lin Jing Luo Chunyan Liu Gil Mor Aihua Liao Source Type: research
Metabolic pathways for glucose and fructose: I synthesis and metabolism of fructose by ovine conceptuses
This study characterized key enzymes involved in fructose synthesis and metabolism by ovine conceptuses throughout pregnancy. Day 17 conceptuses expressed mRNAs for the polyol pathway (SORD and AKR1B1) and glucose and fructose metabolism (HK1, HK2, G6PD, OGT, and FBP), but not those required for gluconeogenesis (G6Pase or PCK). Ovine placentomes also expressed mRNAs for SORD, AKR1B1, HK1, and OGT. Fructose can be metabolized via the ketohexokinase (KHK) pathway and isoforms, KHK-A and KHK-C, were expressed in ovine conceptuses from Day 16 of pregnancy and placentomes during pregnancy in a cell specific manner: KHK-A protei...
Source: Biology of Reproduction - March 19, 2024 Category: Reproduction Medicine Authors: Robyn M Moses Claire Stenhouse Katherine M Halloran Nirvay Sah Emily C Hoskins Shannon E Washburn Gregory A Johnson Guoyao Wu Fuller W Bazer Source Type: research
Pig conceptuses utilize extracellular vesicles for IFNG-mediated paracrine communication with the endometrium
Biol Reprod. 2024 Mar 19:ioae044. doi: 10.1093/biolre/ioae044. Online ahead of print.ABSTRACTInterferon-gamma (IFNG) is a pro-inflammatory cytokine secreted by the porcine conceptus (embryo and extra-embryonic membranes) during the peri-implantation period of pregnancy. IFNG modifies the endometrial inflammatory immune response and is required for the implantation and survival of the conceptus. It is not known how IFNG from the conceptus trophectoderm is transported across the endometrial luminal epithelium (LE). In the present study, immunofluorescence analyses detected immunoreactive IFNG protein in both the trophectoder...
Source: Biology of Reproduction - March 19, 2024 Category: Reproduction Medicine Authors: Joe W Cain Heewon Seo Katie Bumgardner Carli Lefevre Robert C Burghardt Fuller W Bazer Gregory A Johnson Source Type: research
Interleukin-10: a novel metabolic inducer of macrophage differentiation and subsequently contributing to improved pregnancy outcomes of mice by orchestrating oxidative phosphorylation metabolism
In this study, we mainly investigate the metabolic changes involved in IL-10 generated macrophages from monocytes using in vitro models. We demonstrate that exposure of monocytes (either peripheral or THP-1) to IL-10 altered the phenotype and function of resultant macrophages that is linked with OXPHOS changes. IL-10 enhanced the mitochondrial complex I and III activity of THP-1 cell-differentiated macrophages and increased the mitochondrial membrane potential, intracellular adenosine triphosphate, and reactive oxygen species levels. OXPHOS blockage with oligomycin changed the cell morphology of IL-10-generated macrophages...
Source: Biology of Reproduction - March 19, 2024 Category: Reproduction Medicine Authors: Huan Wang Liling Wang Guangshun Gong Xinxiu Lin Jing Luo Chunyan Liu Gil Mor Aihua Liao Source Type: research
Metabolic pathways for glucose and fructose: I synthesis and metabolism of fructose by ovine conceptuses
This study characterized key enzymes involved in fructose synthesis and metabolism by ovine conceptuses throughout pregnancy. Day 17 conceptuses expressed mRNAs for the polyol pathway (SORD and AKR1B1) and glucose and fructose metabolism (HK1, HK2, G6PD, OGT, and FBP), but not those required for gluconeogenesis (G6Pase or PCK). Ovine placentomes also expressed mRNAs for SORD, AKR1B1, HK1, and OGT. Fructose can be metabolized via the ketohexokinase (KHK) pathway and isoforms, KHK-A and KHK-C, were expressed in ovine conceptuses from Day 16 of pregnancy and placentomes during pregnancy in a cell specific manner: KHK-A protei...
Source: Biology of Reproduction - March 19, 2024 Category: Reproduction Medicine Authors: Robyn M Moses Claire Stenhouse Katherine M Halloran Nirvay Sah Emily C Hoskins Shannon E Washburn Gregory A Johnson Guoyao Wu Fuller W Bazer Source Type: research
Pig conceptuses utilize extracellular vesicles for IFNG-mediated paracrine communication with the endometrium
Biol Reprod. 2024 Mar 19:ioae044. doi: 10.1093/biolre/ioae044. Online ahead of print.ABSTRACTInterferon-gamma (IFNG) is a pro-inflammatory cytokine secreted by the porcine conceptus (embryo and extra-embryonic membranes) during the peri-implantation period of pregnancy. IFNG modifies the endometrial inflammatory immune response and is required for the implantation and survival of the conceptus. It is not known how IFNG from the conceptus trophectoderm is transported across the endometrial luminal epithelium (LE). In the present study, immunofluorescence analyses detected immunoreactive IFNG protein in both the trophectoder...
Source: Biology of Reproduction - March 19, 2024 Category: Reproduction Medicine Authors: Joe W Cain Heewon Seo Katie Bumgardner Carli Lefevre Robert C Burghardt Fuller W Bazer Gregory A Johnson Source Type: research
Interleukin-10: a novel metabolic inducer of macrophage differentiation and subsequently contributing to improved pregnancy outcomes of mice by orchestrating oxidative phosphorylation metabolism
In this study, we mainly investigate the metabolic changes involved in IL-10 generated macrophages from monocytes using in vitro models. We demonstrate that exposure of monocytes (either peripheral or THP-1) to IL-10 altered the phenotype and function of resultant macrophages that is linked with OXPHOS changes. IL-10 enhanced the mitochondrial complex I and III activity of THP-1 cell-differentiated macrophages and increased the mitochondrial membrane potential, intracellular adenosine triphosphate, and reactive oxygen species levels. OXPHOS blockage with oligomycin changed the cell morphology of IL-10-generated macrophages...
Source: Biology of Reproduction - March 19, 2024 Category: Reproduction Medicine Authors: Huan Wang Liling Wang Guangshun Gong Xinxiu Lin Jing Luo Chunyan Liu Gil Mor Aihua Liao Source Type: research
Metabolic pathways for glucose and fructose: I synthesis and metabolism of fructose by ovine conceptuses
This study characterized key enzymes involved in fructose synthesis and metabolism by ovine conceptuses throughout pregnancy. Day 17 conceptuses expressed mRNAs for the polyol pathway (SORD and AKR1B1) and glucose and fructose metabolism (HK1, HK2, G6PD, OGT, and FBP), but not those required for gluconeogenesis (G6Pase or PCK). Ovine placentomes also expressed mRNAs for SORD, AKR1B1, HK1, and OGT. Fructose can be metabolized via the ketohexokinase (KHK) pathway and isoforms, KHK-A and KHK-C, were expressed in ovine conceptuses from Day 16 of pregnancy and placentomes during pregnancy in a cell specific manner: KHK-A protei...
Source: Biology of Reproduction - March 19, 2024 Category: Reproduction Medicine Authors: Robyn M Moses Claire Stenhouse Katherine M Halloran Nirvay Sah Emily C Hoskins Shannon E Washburn Gregory A Johnson Guoyao Wu Fuller W Bazer Source Type: research
Cellular senescence in reproduction: a two-edged sword †
Biol Reprod. 2024 Mar 13:ioae025. doi: 10.1093/biolre/ioae025. Online ahead of print.ABSTRACTCellular senescence (CS) is the state when cells are no longer capable to divide even after stimulation with grown factors. Cells that begin to undergo CS stop in the cell cycle and enter a suspended state without committing to programmed cell death. These cells assume a specific phenotype and influence their microenvironment by secreting molecules and extracellular vesicles that are part of the so-called senescent cell-associated secretory phenotype (SASP). Cellular senescence is intertwined with physiological and pathological con...
Source: Biology of Reproduction - March 14, 2024 Category: Reproduction Medicine Authors: Luiza Pretto Eduarda Nabinger Eduardo Cremonesi Filippi-Chiela Lucas Rosa Fraga Source Type: research
