Melatonin-pretreated HucMSCs improved endometrium regeneration and fertility recovery through macrophage immunomodulation in rats with intrauterine adhesions
This study aimed to investigate whether melatonin pretreatment enhances the efficacy of human umbilical cord mesenchymal stem cells (HucMSCs) in IUA treatment in rats. First, we explored the effect of melatonin on the biological activity of HucMSCs in vitro through a macrophage co-culture system, CCK-8, EdU, flow cytometry, immunofluorescence staining, and qRT-PCR. Subsequently, we established the IUA rat model and tracked the distribution of HucMSCs in this model. In addition, we observed the number of M1 and M2 macrophages through immunofluorescence staining and detected the levels of inflammatory cytokines. Four weeks a...
Source: Biology of Reproduction - September 6, 2023 Category: Reproduction Medicine Authors: Weili Qin Jiawei Wang Qianwen Hu Rongyan Qin Nana Ma Fengque Zheng Wencai Tian Jinghang Jiang Ting Li Yufu Jin Ming Liao Aiping Qin Source Type: research

Novel Progestogenic androgens for male contraception: design, synthesis and activity of C7 α-substituted testosterone
Biol Reprod. 2023 Sep 5:ioad111. doi: 10.1093/biolre/ioad111. Online ahead of print.ABSTRACTMale contraceptive development has included use of testosterone (T) with or without a progestin or the use of a single molecule such as progestogenic androgens (PA) for suppression of testicular T production. Expanding upon the vast amount of data accumulated from nortestosterone (NT), NT analogs, and their prodrugs, a new series of progestogenic androgens, the C7 methyl and ethyl α-substituted T analogs 7α-Methyltestosterone (7α-MT) and 7α-Ethyltestosterone (7α-ET), respectively, were hypothesized and designed to have superior...
Source: Biology of Reproduction - September 5, 2023 Category: Reproduction Medicine Authors: Min S Lee Deborah I Bunin Anna M Furimsky Donna Nguyen Toufan Parman Kyuri Kim Linda Rausch Ming-Teh Lin Pranab Gupta Jill E Brown Jeffrey M Kroopnick Diana L Blithe Source Type: research

Relationship of quantitative RT-PCR to RNAseq transcriptome identifies mouse preimplantation embryo reference genes †
Biol Reprod. 2023 Sep 5:ioad107. doi: 10.1093/biolre/ioad107. Online ahead of print.ABSTRACTNumerous reference genes for use with RT-qPCR have been proposed and are commonly used for oocytes, eggs, and preimplantation embryos. However, none of these are actually suitable as reference genes due to their large variations in expression between developmental stages. To address this, we produced a standardized and merged RNAseq data set by combining multiple publicly available RNAseq data sets that spanned mouse GV oocytes, MII eggs, and 1-cell, 2-cell, 4-cell, 8-cell, morula, and blastocyst stage embryos to identify transcript...
Source: Biology of Reproduction - September 5, 2023 Category: Reproduction Medicine Authors: Allison K Tscherner Taylor McClatchie Angus D Macaulay Jay M Baltz Source Type: research

Research progress of the Fanconi anemia pathway and premature ovarian insufficiency
Biol Reprod. 2023 Sep 5:ioad110. doi: 10.1093/biolre/ioad110. Online ahead of print.ABSTRACTThe Fanconi anemia (FA) pathway is a key pathway involved in the repair of DNA interstrand crosslinking (ICL) damage, which chiefly includes the following four modules: lesion recognition, FA core complex recruitment, FANCD2-FANCI complex monoubiquitination, and downstream events (nucleolytic incision, translesion synthesis, and homologous recombination). Mutations or deletions of multiple FA genes in this pathway can damage the ICL repair pathway and disrupt primordial germ cell development and oocyte meiosis, thereby leading to ab...
Source: Biology of Reproduction - September 5, 2023 Category: Reproduction Medicine Authors: Jingyu Zhao Yixin Zhang Wenbo Li Mengmeng Yao Chuqi Liu Zihan Zhang Caiqin Wang Xiaomei Wang Kai Meng Source Type: research

The role of PGRMC2 in the trophoblast invasion
In conclusion, PGRMC2 plays a role in placentation by promoting cell proliferation, invasion, and angiogenesis in EVTs via activation of HIF1α signaling. We thus identified a new function of PGRMC2 and provide insights on understanding the mechanisms of trophoblast invasion.PMID:37665239 | DOI:10.1093/biolre/ioad109 (Source: Biology of Reproduction)
Source: Biology of Reproduction - September 4, 2023 Category: Reproduction Medicine Authors: Tae Yokouchi-Konishi Yongjie Liu Liping Feng Source Type: research