Association between ACE1 and missed abortion: ACE1 promotes H2O2-induced trophoblast cell injury in vitro †
Biol Reprod. 2024 Apr 1:ioae049. doi: 10.1093/biolre/ioae049. Online ahead of print.ABSTRACTThe aim of this study was to evaluate the role of angiotensin-converting enzyme 1 (ACE1) in H2O2-induced trophoblast cell injury and the potential molecular mechanisms. Oxidative stress was modeled by exposing HTR-8/SVneo cells to 200 μM H2O2. Western blot and real-time quantitative PCR methods were used to detect protein and mRNA expression level of ACE1 in chorionic villus tissue and trophoblast HTR-8/SVneo cell. Inhibition of ACE1 expression was achieved by transfection with small interfering RNA. Then flow cytometry, Cell Count...
Source: Biology of Reproduction - April 6, 2024 Category: Reproduction Medicine Authors: Yi-Dan Yan Wen-Xia Ji Na Zhao Jian-Juan Zhang Jie Du Ting Lu Wei-Qun Gu Source Type: research
Association between ACE1 and missed abortion: ACE1 promotes H2O2-induced trophoblast cell injury in vitro †
Biol Reprod. 2024 Apr 1:ioae049. doi: 10.1093/biolre/ioae049. Online ahead of print.ABSTRACTThe aim of this study was to evaluate the role of angiotensin-converting enzyme 1 (ACE1) in H2O2-induced trophoblast cell injury and the potential molecular mechanisms. Oxidative stress was modeled by exposing HTR-8/SVneo cells to 200 μM H2O2. Western blot and real-time quantitative PCR methods were used to detect protein and mRNA expression level of ACE1 in chorionic villus tissue and trophoblast HTR-8/SVneo cell. Inhibition of ACE1 expression was achieved by transfection with small interfering RNA. Then flow cytometry, Cell Count...
Source: Biology of Reproduction - April 6, 2024 Category: Reproduction Medicine Authors: Yi-Dan Yan Wen-Xia Ji Na Zhao Jian-Juan Zhang Jie Du Ting Lu Wei-Qun Gu Source Type: research
Association between ACE1 and missed abortion: ACE1 promotes H2O2-induced trophoblast cell injury in vitro †
Biol Reprod. 2024 Apr 1:ioae049. doi: 10.1093/biolre/ioae049. Online ahead of print.ABSTRACTThe aim of this study was to evaluate the role of angiotensin-converting enzyme 1 (ACE1) in H2O2-induced trophoblast cell injury and the potential molecular mechanisms. Oxidative stress was modeled by exposing HTR-8/SVneo cells to 200 μM H2O2. Western blot and real-time quantitative PCR methods were used to detect protein and mRNA expression level of ACE1 in chorionic villus tissue and trophoblast HTR-8/SVneo cell. Inhibition of ACE1 expression was achieved by transfection with small interfering RNA. Then flow cytometry, Cell Count...
Source: Biology of Reproduction - April 6, 2024 Category: Reproduction Medicine Authors: Yi-Dan Yan Wen-Xia Ji Na Zhao Jian-Juan Zhang Jie Du Ting Lu Wei-Qun Gu Source Type: research
Quantification of follicles in human ovarian tissue using image processing software and trained artificial intelligence
Biol Reprod. 2024 Mar 27:ioae048. doi: 10.1093/biolre/ioae048. Online ahead of print.ABSTRACTCancer survival rates in prepubertal girls and young women have risen in recent decades due to increasingly efficient treatments. However, many such treatments are gonadotoxic, causing premature ovarian insufficiency (POI), loss of fertility and ovarian endocrine function. Implantation of donor ovarian tissue encapsulated in immune-isolating capsules is a promising method to restore physiological endocrine function without immunosuppression or risk of reintroducing cancer cells harbored by the tissue. The success of this approach i...
Source: Biology of Reproduction - March 27, 2024 Category: Reproduction Medicine Authors: Gabrielle M Blevins Colleen L Flanagan Sridula S Kallakuri Owen M Meyer Likitha Nimmagadda James D Hatch Sydney A Shea Vasantha Padmanabhan Ariella Shikanov Source Type: research
Quantification of follicles in human ovarian tissue using image processing software and trained artificial intelligence
Biol Reprod. 2024 Mar 27:ioae048. doi: 10.1093/biolre/ioae048. Online ahead of print.ABSTRACTCancer survival rates in prepubertal girls and young women have risen in recent decades due to increasingly efficient treatments. However, many such treatments are gonadotoxic, causing premature ovarian insufficiency (POI), loss of fertility and ovarian endocrine function. Implantation of donor ovarian tissue encapsulated in immune-isolating capsules is a promising method to restore physiological endocrine function without immunosuppression or risk of reintroducing cancer cells harbored by the tissue. The success of this approach i...
Source: Biology of Reproduction - March 27, 2024 Category: Reproduction Medicine Authors: Gabrielle M Blevins Colleen L Flanagan Sridula S Kallakuri Owen M Meyer Likitha Nimmagadda James D Hatch Sydney A Shea Vasantha Padmanabhan Ariella Shikanov Source Type: research
Quantification of follicles in human ovarian tissue using image processing software and trained artificial intelligence
Biol Reprod. 2024 Mar 27:ioae048. doi: 10.1093/biolre/ioae048. Online ahead of print.ABSTRACTCancer survival rates in prepubertal girls and young women have risen in recent decades due to increasingly efficient treatments. However, many such treatments are gonadotoxic, causing premature ovarian insufficiency (POI), loss of fertility and ovarian endocrine function. Implantation of donor ovarian tissue encapsulated in immune-isolating capsules is a promising method to restore physiological endocrine function without immunosuppression or risk of reintroducing cancer cells harbored by the tissue. The success of this approach i...
Source: Biology of Reproduction - March 27, 2024 Category: Reproduction Medicine Authors: Gabrielle M Blevins Colleen L Flanagan Sridula S Kallakuri Owen M Meyer Likitha Nimmagadda James D Hatch Sydney A Shea Vasantha Padmanabhan Ariella Shikanov Source Type: research
Quantification of follicles in human ovarian tissue using image processing software and trained artificial intelligence
Biol Reprod. 2024 Mar 27:ioae048. doi: 10.1093/biolre/ioae048. Online ahead of print.ABSTRACTCancer survival rates in prepubertal girls and young women have risen in recent decades due to increasingly efficient treatments. However, many such treatments are gonadotoxic, causing premature ovarian insufficiency (POI), loss of fertility and ovarian endocrine function. Implantation of donor ovarian tissue encapsulated in immune-isolating capsules is a promising method to restore physiological endocrine function without immunosuppression or risk of reintroducing cancer cells harbored by the tissue. The success of this approach i...
Source: Biology of Reproduction - March 27, 2024 Category: Reproduction Medicine Authors: Gabrielle M Blevins Colleen L Flanagan Sridula S Kallakuri Owen M Meyer Likitha Nimmagadda James D Hatch Sydney A Shea Vasantha Padmanabhan Ariella Shikanov Source Type: research
Quantification of follicles in human ovarian tissue using image processing software and trained artificial intelligence
Biol Reprod. 2024 Mar 27:ioae048. doi: 10.1093/biolre/ioae048. Online ahead of print.ABSTRACTCancer survival rates in prepubertal girls and young women have risen in recent decades due to increasingly efficient treatments. However, many such treatments are gonadotoxic, causing premature ovarian insufficiency (POI), loss of fertility and ovarian endocrine function. Implantation of donor ovarian tissue encapsulated in immune-isolating capsules is a promising method to restore physiological endocrine function without immunosuppression or risk of reintroducing cancer cells harbored by the tissue. The success of this approach i...
Source: Biology of Reproduction - March 27, 2024 Category: Reproduction Medicine Authors: Gabrielle M Blevins Colleen L Flanagan Sridula S Kallakuri Owen M Meyer Likitha Nimmagadda James D Hatch Sydney A Shea Vasantha Padmanabhan Ariella Shikanov Source Type: research
Quantification of follicles in human ovarian tissue using image processing software and trained artificial intelligence
Biol Reprod. 2024 Mar 27:ioae048. doi: 10.1093/biolre/ioae048. Online ahead of print.ABSTRACTCancer survival rates in prepubertal girls and young women have risen in recent decades due to increasingly efficient treatments. However, many such treatments are gonadotoxic, causing premature ovarian insufficiency (POI), loss of fertility and ovarian endocrine function. Implantation of donor ovarian tissue encapsulated in immune-isolating capsules is a promising method to restore physiological endocrine function without immunosuppression or risk of reintroducing cancer cells harbored by the tissue. The success of this approach i...
Source: Biology of Reproduction - March 27, 2024 Category: Reproduction Medicine Authors: Gabrielle M Blevins Colleen L Flanagan Sridula S Kallakuri Owen M Meyer Likitha Nimmagadda James D Hatch Sydney A Shea Vasantha Padmanabhan Ariella Shikanov Source Type: research
Quantification of follicles in human ovarian tissue using image processing software and trained artificial intelligence
Biol Reprod. 2024 Mar 27:ioae048. doi: 10.1093/biolre/ioae048. Online ahead of print.ABSTRACTCancer survival rates in prepubertal girls and young women have risen in recent decades due to increasingly efficient treatments. However, many such treatments are gonadotoxic, causing premature ovarian insufficiency (POI), loss of fertility and ovarian endocrine function. Implantation of donor ovarian tissue encapsulated in immune-isolating capsules is a promising method to restore physiological endocrine function without immunosuppression or risk of reintroducing cancer cells harbored by the tissue. The success of this approach i...
Source: Biology of Reproduction - March 27, 2024 Category: Reproduction Medicine Authors: Gabrielle M Blevins Colleen L Flanagan Sridula S Kallakuri Owen M Meyer Likitha Nimmagadda James D Hatch Sydney A Shea Vasantha Padmanabhan Ariella Shikanov Source Type: research
Quantification of follicles in human ovarian tissue using image processing software and trained artificial intelligence
Biol Reprod. 2024 Mar 27:ioae048. doi: 10.1093/biolre/ioae048. Online ahead of print.ABSTRACTCancer survival rates in prepubertal girls and young women have risen in recent decades due to increasingly efficient treatments. However, many such treatments are gonadotoxic, causing premature ovarian insufficiency (POI), loss of fertility and ovarian endocrine function. Implantation of donor ovarian tissue encapsulated in immune-isolating capsules is a promising method to restore physiological endocrine function without immunosuppression or risk of reintroducing cancer cells harbored by the tissue. The success of this approach i...
Source: Biology of Reproduction - March 27, 2024 Category: Reproduction Medicine Authors: Gabrielle M Blevins Colleen L Flanagan Sridula S Kallakuri Owen M Meyer Likitha Nimmagadda James D Hatch Sydney A Shea Vasantha Padmanabhan Ariella Shikanov Source Type: research
Metabolic pathways of glucose and fructose: II spatiotemporal expression of genes involved in synthesis and transport of lactate in ovine conceptuses
This study characterized total lactate produced by ovine conceptuses throughout gestation, as well as expression of mRNAs and proteins involved in lactate metabolism. Lactate increased in abundance in the uterine lumen during the preimplantation period and was more abundant than pyruvate. The abundance of lactate in allantoic and amniotic fluids increased with advancing days of gestation and most abundant on Day 125 of pregnancy (P < 0.05). Lactate dehydrogenase (LDH) subunits A (converts pyruvate to lactate) and B (converts lactate to pyruvate) were expressed by conceptuses throughout gestation. Lactate is transported ...
Source: Biology of Reproduction - March 26, 2024 Category: Reproduction Medicine Authors: Robyn M Moses Claire Stenhouse Katherine M Halloran Nirvay Sah Makenzie G Newton Emily C Hoskins Shannon E Washburn Gregory A Johnson Guoyao Wu Fuller W Bazer Source Type: research
Clinical and gonadal transcriptome analysis of 38,XX disorder of sex development pigs
This study aims to investigate the phenotypic characteristics and molecular mechanisms underlying the reproductive and developmental abnormalities of 38,XX ovotestis-DSD (OT-DSD) and 38,XX testis-DSD (T-DSD) in pigs. Clinical and transcriptome sequencing analyses were performed on DSD and normal female pigs. Cytogenetic and SRY analyses confirmed that OT/T-DSD pigs exhibited a 38,XX karyotype and lacked the SRY gene. The DSD pigs had higher levels of follicle-stimulating hormone, luteinizing hormone, and progesterone, but lower testosterone levels when compared with normal male pigs. The reproductive organs of OT/T-DSD pig...
Source: Biology of Reproduction - March 26, 2024 Category: Reproduction Medicine Authors: Jinhua Wu Shuwen Tan Yi Zhou Haiquan Zhao Hui Yu Bingzhou Zhong Congying Yu Haoming Wang Yin Yang Hua Li Yugu Li Source Type: research
In Remembrance of a prolific reproductive biologist, editor of Biology of Reproduction, and enthusiastic supporter of young investigators †
Biol Reprod. 2024 Mar 25:ioae022. doi: 10.1093/biolre/ioae022. Online ahead of print.NO ABSTRACTPMID:38526867 | DOI:10.1093/biolre/ioae022 (Source: Biology of Reproduction)
Source: Biology of Reproduction - March 25, 2024 Category: Reproduction Medicine Authors: J Charles Eldridge Rajagopala Sridaran Darrell Brann Source Type: research
In Remembrance of a prolific reproductive biologist, editor of Biology of Reproduction, and enthusiastic supporter of young investigators †
Biol Reprod. 2024 Mar 25:ioae022. doi: 10.1093/biolre/ioae022. Online ahead of print.NO ABSTRACTPMID:38526867 | DOI:10.1093/biolre/ioae022 (Source: Biology of Reproduction)
Source: Biology of Reproduction - March 25, 2024 Category: Reproduction Medicine Authors: J Charles Eldridge Rajagopala Sridaran Darrell Brann Source Type: research
