Dysregulation of MTFR2, ATP5IF1 and BAK1 in Sertoli cells relates to idiopathic non-obstructive azoospermia via inhibiting mitochondrial fission and inducing mitochondrial dysfunction
In this study, we checked the morphology of Sertoli cell (SC) mitochondria in testis biopsies from patients with iNOA and patients with obstructive azoospermia (OA) who have normal spermiogenesis. The expression of 104 genes controlling mitochondria fission and fusion were analyzed in three gene expression datasets including a total of 60 patients with NOA. The levels of 7 candidate genes were detected in testis biopsies from 38 patients with iNOA and 24 patients with OA who have normal spermatogenesis by RT-qPCR. Cell viability, apoptosis, mitochondria membrane potential, ATP production, oxygen consumption and mitochondri...
Source: Biology of Reproduction - October 30, 2023 Category: Reproduction Medicine Authors: Lei Wei Zonggang Feng Qian Dou Li Tan Xinghua Zhao Bin Hao Source Type: research
Group B streptococcus induces cellular senescence in human amnion epithelial cells through a partial interleukin-1-mediated mechanism
Biol Reprod. 2023 Oct 30:ioad149. doi: 10.1093/biolre/ioad149. Online ahead of print.ABSTRACTGroup B streptococcus (GBS) infection is a significant public health concern associated with adverse pregnancy complications and increased neonatal mortality and morbidity. However, the mechanisms underlying the impact of GBS on the fetal membrane, the first line of defense against pathogens, are not fully understood. Here, we propose that GBS induces senescence and inflammatory factors (IL-6 and IL-8) in the fetal membrane through interleukin-1 (IL-1). Utilizing the existing transcriptomic data on GBS-exposed human fetal membrane,...
Source: Biology of Reproduction - October 30, 2023 Category: Reproduction Medicine Authors: Hae-Ryung Park Kelly A Hogan Sean M Harris Mark C Chames Rita Loch-Caruso Source Type: research
Extracellular vesicles from mouse trophoblast cells: effects on neural progenitor cells and potential participants in the placenta-brain Axis
Biol Reprod. 2023 Oct 26:ioad146. doi: 10.1093/biolre/ioad146. Online ahead of print.ABSTRACTThe fetal brain of the mouse is thought to be dependent upon the placenta as a source of serotonin (5-hydroxytryptamine; 5-HT) and other factors. How factors reach the developing brain remains uncertain but are postulated here to be part of the cargo carried by placental extracellular vesicles (EV). We have analyzed the protein, catecholamine, and small RNA content of EV from mouse trophoblast stem cells (TSC) and TSC differentiated into parietal trophoblast giant cells (pTGC), potential primary purveyors of 5-HT. We have examined ...
Source: Biology of Reproduction - October 26, 2023 Category: Reproduction Medicine Authors: Jessica A Kinkade Arun S Seetharam Shrikesh Sachdev Nathan J Bivens Brett S Phinney Gabriela Grigorean R Michael Roberts Geetu Tuteja Cheryl S Rosenfeld Source Type: research
Extracellular vesicles from mouse trophoblast cells: effects on neural progenitor cells and potential participants in the placenta-brain Axis
Biol Reprod. 2023 Oct 26:ioad146. doi: 10.1093/biolre/ioad146. Online ahead of print.ABSTRACTThe fetal brain of the mouse is thought to be dependent upon the placenta as a source of serotonin (5-hydroxytryptamine; 5-HT) and other factors. How factors reach the developing brain remains uncertain but are postulated here to be part of the cargo carried by placental extracellular vesicles (EV). We have analyzed the protein, catecholamine, and small RNA content of EV from mouse trophoblast stem cells (TSC) and TSC differentiated into parietal trophoblast giant cells (pTGC), potential primary purveyors of 5-HT. We have examined ...
Source: Biology of Reproduction - October 26, 2023 Category: Reproduction Medicine Authors: Jessica A Kinkade Arun S Seetharam Shrikesh Sachdev Nathan J Bivens Brett S Phinney Gabriela Grigorean R Michael Roberts Geetu Tuteja Cheryl S Rosenfeld Source Type: research
Extracellular vesicles from mouse trophoblast cells: effects on neural progenitor cells and potential participants in the placenta-brain Axis
Biol Reprod. 2023 Oct 26:ioad146. doi: 10.1093/biolre/ioad146. Online ahead of print.ABSTRACTThe fetal brain of the mouse is thought to be dependent upon the placenta as a source of serotonin (5-hydroxytryptamine; 5-HT) and other factors. How factors reach the developing brain remains uncertain but are postulated here to be part of the cargo carried by placental extracellular vesicles (EV). We have analyzed the protein, catecholamine, and small RNA content of EV from mouse trophoblast stem cells (TSC) and TSC differentiated into parietal trophoblast giant cells (pTGC), potential primary purveyors of 5-HT. We have examined ...
Source: Biology of Reproduction - October 26, 2023 Category: Reproduction Medicine Authors: Jessica A Kinkade Arun S Seetharam Shrikesh Sachdev Nathan J Bivens Brett S Phinney Gabriela Grigorean R Michael Roberts Geetu Tuteja Cheryl S Rosenfeld Source Type: research
Extracellular vesicles from mouse trophoblast cells: effects on neural progenitor cells and potential participants in the placenta-brain Axis
Biol Reprod. 2023 Oct 26:ioad146. doi: 10.1093/biolre/ioad146. Online ahead of print.ABSTRACTThe fetal brain of the mouse is thought to be dependent upon the placenta as a source of serotonin (5-hydroxytryptamine; 5-HT) and other factors. How factors reach the developing brain remains uncertain but are postulated here to be part of the cargo carried by placental extracellular vesicles (EV). We have analyzed the protein, catecholamine, and small RNA content of EV from mouse trophoblast stem cells (TSC) and TSC differentiated into parietal trophoblast giant cells (pTGC), potential primary purveyors of 5-HT. We have examined ...
Source: Biology of Reproduction - October 26, 2023 Category: Reproduction Medicine Authors: Jessica A Kinkade Arun S Seetharam Shrikesh Sachdev Nathan J Bivens Brett S Phinney Gabriela Grigorean R Michael Roberts Geetu Tuteja Cheryl S Rosenfeld Source Type: research
Clustering of multi tissue transcriptomes in gilts with normal cyclicity or delayed puberty reveals genes related to pubertal development
Biol Reprod. 2023 Oct 23:ioad145. doi: 10.1093/biolre/ioad145. Online ahead of print.ABSTRACTIn gilts, puberty is marked by standing estrus in the presence of a boar. Delayed puberty (failure to display pubertal estrus) is a major reason for gilt removal. To investigate the physiological determinants underlying delayed puberty in gilts, transcriptomic data from tissues relevant to estrus and puberty, such as mediobasal hypothalamus, anterior pituitary gland, ovarian cortex, olfactory bulb, amygdala, and hippocampus were obtained from age-matched delayed puberty (n = 8) and cyclic control gilts at follicular phase (n = 8) a...
Source: Biology of Reproduction - October 23, 2023 Category: Reproduction Medicine Authors: Hiruni R Wijesena Brittney N Keel Dan J Nonneman Robert A Cushman Clay A Lents Source Type: research
Clustering of multi tissue transcriptomes in gilts with normal cyclicity or delayed puberty reveals genes related to pubertal development
Biol Reprod. 2023 Oct 23:ioad145. doi: 10.1093/biolre/ioad145. Online ahead of print.ABSTRACTIn gilts, puberty is marked by standing estrus in the presence of a boar. Delayed puberty (failure to display pubertal estrus) is a major reason for gilt removal. To investigate the physiological determinants underlying delayed puberty in gilts, transcriptomic data from tissues relevant to estrus and puberty, such as mediobasal hypothalamus, anterior pituitary gland, ovarian cortex, olfactory bulb, amygdala, and hippocampus were obtained from age-matched delayed puberty (n = 8) and cyclic control gilts at follicular phase (n = 8) a...
Source: Biology of Reproduction - October 23, 2023 Category: Reproduction Medicine Authors: Hiruni R Wijesena Brittney N Keel Dan J Nonneman Robert A Cushman Clay A Lents Source Type: research
Clustering of multi tissue transcriptomes in gilts with normal cyclicity or delayed puberty reveals genes related to pubertal development
Biol Reprod. 2023 Oct 23:ioad145. doi: 10.1093/biolre/ioad145. Online ahead of print.ABSTRACTIn gilts, puberty is marked by standing estrus in the presence of a boar. Delayed puberty (failure to display pubertal estrus) is a major reason for gilt removal. To investigate the physiological determinants underlying delayed puberty in gilts, transcriptomic data from tissues relevant to estrus and puberty, such as mediobasal hypothalamus, anterior pituitary gland, ovarian cortex, olfactory bulb, amygdala, and hippocampus were obtained from age-matched delayed puberty (n = 8) and cyclic control gilts at follicular phase (n = 8) a...
Source: Biology of Reproduction - October 23, 2023 Category: Reproduction Medicine Authors: Hiruni R Wijesena Brittney N Keel Dan J Nonneman Robert A Cushman Clay A Lents Source Type: research
Clustering of multi tissue transcriptomes in gilts with normal cyclicity or delayed puberty reveals genes related to pubertal development
Biol Reprod. 2023 Oct 23:ioad145. doi: 10.1093/biolre/ioad145. Online ahead of print.ABSTRACTIn gilts, puberty is marked by standing estrus in the presence of a boar. Delayed puberty (failure to display pubertal estrus) is a major reason for gilt removal. To investigate the physiological determinants underlying delayed puberty in gilts, transcriptomic data from tissues relevant to estrus and puberty, such as mediobasal hypothalamus, anterior pituitary gland, ovarian cortex, olfactory bulb, amygdala, and hippocampus were obtained from age-matched delayed puberty (n = 8) and cyclic control gilts at follicular phase (n = 8) a...
Source: Biology of Reproduction - October 23, 2023 Category: Reproduction Medicine Authors: Hiruni R Wijesena Brittney N Keel Dan J Nonneman Robert A Cushman Clay A Lents Source Type: research
KDM6A/UTX promotes spermatogenic gene expression across generations and is not required for male fertility
Biol Reprod. 2023 Oct 20:ioad141. doi: 10.1093/biolre/ioad141. Online ahead of print.ABSTRACTPaternal chromatin undergoes extensive structural and epigenetic changes during mammalian spermatogenesis, producing sperm with an epigenome optimized for the transition to embryogenesis. Lysine demethylase 6a (KDM6A, also called UTX) promotes gene activation in part via demethylation of H3K27me3, a developmentally important repressive modification abundant throughout the epigenome of spermatogenic cells and sperm. We previously demonstrated increased cancer risk in genetically wild type mice derived from a paternal germ line lacki...
Source: Biology of Reproduction - October 20, 2023 Category: Reproduction Medicine Authors: Benjamin William Walters Shannon R Rainsford Rachel A Heuer Nicolas Dias Xiaofang Huang Dirk de Rooij Bluma J Lesch Source Type: research
KDM6A/UTX promotes spermatogenic gene expression across generations and is not required for male fertility
Biol Reprod. 2023 Oct 20:ioad141. doi: 10.1093/biolre/ioad141. Online ahead of print.ABSTRACTPaternal chromatin undergoes extensive structural and epigenetic changes during mammalian spermatogenesis, producing sperm with an epigenome optimized for the transition to embryogenesis. Lysine demethylase 6a (KDM6A, also called UTX) promotes gene activation in part via demethylation of H3K27me3, a developmentally important repressive modification abundant throughout the epigenome of spermatogenic cells and sperm. We previously demonstrated increased cancer risk in genetically wild type mice derived from a paternal germ line lacki...
Source: Biology of Reproduction - October 20, 2023 Category: Reproduction Medicine Authors: Benjamin William Walters Shannon R Rainsford Rachel A Heuer Nicolas Dias Xiaofang Huang Dirk de Rooij Bluma J Lesch Source Type: research
KDM6A/UTX promotes spermatogenic gene expression across generations and is not required for male fertility
Biol Reprod. 2023 Oct 20:ioad141. doi: 10.1093/biolre/ioad141. Online ahead of print.ABSTRACTPaternal chromatin undergoes extensive structural and epigenetic changes during mammalian spermatogenesis, producing sperm with an epigenome optimized for the transition to embryogenesis. Lysine demethylase 6a (KDM6A, also called UTX) promotes gene activation in part via demethylation of H3K27me3, a developmentally important repressive modification abundant throughout the epigenome of spermatogenic cells and sperm. We previously demonstrated increased cancer risk in genetically wild type mice derived from a paternal germ line lacki...
Source: Biology of Reproduction - October 20, 2023 Category: Reproduction Medicine Authors: Benjamin William Walters Shannon R Rainsford Rachel A Heuer Nicolas Dias Xiaofang Huang Dirk de Rooij Bluma J Lesch Source Type: research
Altered histone abundance as a mode of ovotoxicity during 7,12-dimethylbenz[a]anthracene exposure with additive influence of obesity
The objective of this study was to identify ovarian proteomic and histone modifications induced by DMBA exposure and to examine any influence of obesity thereon. Post-pubertal wildtype (KK.Cg-a/a; lean) and agouti (KK.Cg-Ay/J; obese) female mice, were exposed to either corn oil (CT) or DMBA (1 mg/kg) for 7d via intraperitoneal injection (n = 10/treatment). Total ovarian proteome analysis (LC-MS/MS) determined that obesity altered a total of 225 proteins (P < 0.05) and histone 3 was the second least abundant protein (FC = -5.98, P < 0.05). Histone 4 was decreased by 3.33-fold whereas histone variant H3.3 was decreased...
Source: Biology of Reproduction - October 19, 2023 Category: Reproduction Medicine Authors: Jaspreet K Rishi Kelsey Timme Hunter E White Karl C Kerns Aileen F Keating Source Type: research
Luteinizing hormone stimulates ingression of mural granulosa cells within the mouse preovulatory follicle
Biol Reprod. 2023 Oct 17:ioad142. doi: 10.1093/biolre/ioad142. Online ahead of print.ABSTRACTLuteinizing hormone (LH) induces ovulation by acting on its receptors in the mural granulosa cells that surround a mammalian oocyte in an ovarian follicle. However, much remains unknown about how activation of the LH receptor modifies the structure of the follicle such that the oocyte is released and the follicle remnants are transformed into the corpus luteum. The present study shows that the preovulatory surge of LH stimulates LH receptor-expressing granulosa cells, initially located almost entirely in the outer layers of the mur...
Source: Biology of Reproduction - October 17, 2023 Category: Reproduction Medicine Authors: Corie M Owen Laurinda A Jaffe Source Type: research
