An interview with Dr David Albertini
Biol Reprod. 2023 Sep 6:ioad115. doi: 10.1093/biolre/ioad115. Online ahead of print.NO ABSTRACTPMID:37738136 | DOI:10.1093/biolre/ioad115 (Source: Biology of Reproduction)
Source: Biology of Reproduction - September 22, 2023 Category: Reproduction Medicine Authors: T Rajendra Kumar Source Type: research
Polystyrene micro- and nanoplastics cause placental dysfunction in mice1
In this study, we investigated the placental and fetal hemodynamic responses to plastics exposure in mice using high-frequency ultrasound. Healthy, pregnant CD-1 dams were given either 106 ng/L of 5 μm polystyrene MPs or 106 ng/L of 50 nm polystyrene NPs in drinking water throughout gestation and compared with controls. Maternal exposure to both MPs and NPs resulted in evidence of placental dysfunction that was highly dependent on the particle size. The umbilical artery blood flow increased by 48% in the MP-exposed group and decreased by 25% in the NP-exposed group compared to controls (p < 0.05). The MP- and NP-expose...
Source: Biology of Reproduction - September 19, 2023 Category: Reproduction Medicine Authors: Katherine C Dibbon Grace V Mercer Alexandre S Maekawa Jenna Hanrahan Katherine L Steeves Lauren C M Ringer Andr é J Simpson Myrna J Simpson Ahmet A Baschat John C Kingdom Christopher K Macgowan John G Sled Karl J Jobst Lindsay S Cahill Source Type: research
Placental insufficiency and heavier placentas in sheep after suppressing CXCL12/CXCR4 signaling during implantation
The objectives were to determine if 1) suppressing CXCL12/CXCR4 during implantation results in reduced fetal and placental growth and development and 2) if varying the amount of time CXCL12/CXCR4 is suppressed impacts fetal/placental development. Fetal weights were similar, however greater placental weight and placentome numbers occurred when CXCL12/CXCR4 was suppressed for 14 days. In caruncles, greater abundance of fibroblast growth factor 2 (FGF2), vascular endothelial growth factor (VEGF-A), VEGF-A receptor 1 (FLT-1), and placental growth factor (PLGF) were observed after suppressing CXCL12/CXCR4. Similar results occur...
Source: Biology of Reproduction - September 19, 2023 Category: Reproduction Medicine Authors: R L Ashley E M Trigo J M Ervin Source Type: research
SMAD signaling pathway is disrupted by BPA via the AMH receptor in bovine granulosa cells
Biol Reprod. 2023 Sep 19:ioad125. doi: 10.1093/biolre/ioad125. Online ahead of print.ABSTRACTSignificant events that determine oocyte competence occur during follicular growth and oocyte maturation. The Anti-Mullerian hormone (AMH), a positive predictor of fertility, has been shown to be affected by exposure to endocrine disrupting compounds (EDCs), such as bisphenol A (BPA) and S (BPS). However, the interaction between bisphenols and SMAD proteins, mediators of the AMH pathway, has not yet been elucidated. AMH receptor (AMHRII) and downstream SMADs expression was investigated in bovine granulosa cells treated with BPA, BP...
Source: Biology of Reproduction - September 19, 2023 Category: Reproduction Medicine Authors: Rushi H Patel Vivien B Truong Reem Sabry Julianna E Acosta Kiera McCahill Laura A Favetta Source Type: research
Polystyrene micro- and nanoplastics cause placental dysfunction in mice1
In this study, we investigated the placental and fetal hemodynamic responses to plastics exposure in mice using high-frequency ultrasound. Healthy, pregnant CD-1 dams were given either 106 ng/L of 5 μm polystyrene MPs or 106 ng/L of 50 nm polystyrene NPs in drinking water throughout gestation and compared with controls. Maternal exposure to both MPs and NPs resulted in evidence of placental dysfunction that was highly dependent on the particle size. The umbilical artery blood flow increased by 48% in the MP-exposed group and decreased by 25% in the NP-exposed group compared to controls (p < 0.05). The MP- and NP-expose...
Source: Biology of Reproduction - September 19, 2023 Category: Reproduction Medicine Authors: Katherine C Dibbon Grace V Mercer Alexandre S Maekawa Jenna Hanrahan Katherine L Steeves Lauren C M Ringer Andr é J Simpson Myrna J Simpson Ahmet A Baschat John C Kingdom Christopher K Macgowan John G Sled Karl J Jobst Lindsay S Cahill Source Type: research
Placental insufficiency and heavier placentas in sheep after suppressing CXCL12/CXCR4 signaling during implantation
The objectives were to determine if 1) suppressing CXCL12/CXCR4 during implantation results in reduced fetal and placental growth and development and 2) if varying the amount of time CXCL12/CXCR4 is suppressed impacts fetal/placental development. Fetal weights were similar, however greater placental weight and placentome numbers occurred when CXCL12/CXCR4 was suppressed for 14 days. In caruncles, greater abundance of fibroblast growth factor 2 (FGF2), vascular endothelial growth factor (VEGF-A), VEGF-A receptor 1 (FLT-1), and placental growth factor (PLGF) were observed after suppressing CXCL12/CXCR4. Similar results occur...
Source: Biology of Reproduction - September 19, 2023 Category: Reproduction Medicine Authors: R L Ashley E M Trigo J M Ervin Source Type: research
SMAD signaling pathway is disrupted by BPA via the AMH receptor in bovine granulosa cells
Biol Reprod. 2023 Sep 19:ioad125. doi: 10.1093/biolre/ioad125. Online ahead of print.ABSTRACTSignificant events that determine oocyte competence occur during follicular growth and oocyte maturation. The Anti-Mullerian hormone (AMH), a positive predictor of fertility, has been shown to be affected by exposure to endocrine disrupting compounds (EDCs), such as bisphenol A (BPA) and S (BPS). However, the interaction between bisphenols and SMAD proteins, mediators of the AMH pathway, has not yet been elucidated. AMH receptor (AMHRII) and downstream SMADs expression was investigated in bovine granulosa cells treated with BPA, BP...
Source: Biology of Reproduction - September 19, 2023 Category: Reproduction Medicine Authors: Rushi H Patel Vivien B Truong Reem Sabry Julianna E Acosta Kiera McCahill Laura A Favetta Source Type: research
Polystyrene micro- and nanoplastics cause placental dysfunction in mice1
In this study, we investigated the placental and fetal hemodynamic responses to plastics exposure in mice using high-frequency ultrasound. Healthy, pregnant CD-1 dams were given either 106 ng/L of 5 μm polystyrene MPs or 106 ng/L of 50 nm polystyrene NPs in drinking water throughout gestation and compared with controls. Maternal exposure to both MPs and NPs resulted in evidence of placental dysfunction that was highly dependent on the particle size. The umbilical artery blood flow increased by 48% in the MP-exposed group and decreased by 25% in the NP-exposed group compared to controls (p < 0.05). The MP- and NP-expose...
Source: Biology of Reproduction - September 19, 2023 Category: Reproduction Medicine Authors: Katherine C Dibbon Grace V Mercer Alexandre S Maekawa Jenna Hanrahan Katherine L Steeves Lauren C M Ringer Andr é J Simpson Myrna J Simpson Ahmet A Baschat John C Kingdom Christopher K Macgowan John G Sled Karl J Jobst Lindsay S Cahill Source Type: research
Placental insufficiency and heavier placentas in sheep after suppressing CXCL12/CXCR4 signaling during implantation
The objectives were to determine if 1) suppressing CXCL12/CXCR4 during implantation results in reduced fetal and placental growth and development and 2) if varying the amount of time CXCL12/CXCR4 is suppressed impacts fetal/placental development. Fetal weights were similar, however greater placental weight and placentome numbers occurred when CXCL12/CXCR4 was suppressed for 14 days. In caruncles, greater abundance of fibroblast growth factor 2 (FGF2), vascular endothelial growth factor (VEGF-A), VEGF-A receptor 1 (FLT-1), and placental growth factor (PLGF) were observed after suppressing CXCL12/CXCR4. Similar results occur...
Source: Biology of Reproduction - September 19, 2023 Category: Reproduction Medicine Authors: R L Ashley E M Trigo J M Ervin Source Type: research
SMAD signaling pathway is disrupted by BPA via the AMH receptor in bovine granulosa cells
Biol Reprod. 2023 Sep 19:ioad125. doi: 10.1093/biolre/ioad125. Online ahead of print.ABSTRACTSignificant events that determine oocyte competence occur during follicular growth and oocyte maturation. The Anti-Mullerian hormone (AMH), a positive predictor of fertility, has been shown to be affected by exposure to endocrine disrupting compounds (EDCs), such as bisphenol A (BPA) and S (BPS). However, the interaction between bisphenols and SMAD proteins, mediators of the AMH pathway, has not yet been elucidated. AMH receptor (AMHRII) and downstream SMADs expression was investigated in bovine granulosa cells treated with BPA, BP...
Source: Biology of Reproduction - September 19, 2023 Category: Reproduction Medicine Authors: Rushi H Patel Vivien B Truong Reem Sabry Julianna E Acosta Kiera McCahill Laura A Favetta Source Type: research
Involvement of cathepsin L in the degradation and degeneration of postovulatory follicle of the medaka ovary
Biol Reprod. 2023 Sep 15:ioad116. doi: 10.1093/biolre/ioad116. Online ahead of print.ABSTRACTCathepsin L (CTSL) plays physiological and pathological roles in immune responses, cancer, metamorphosis, and oogenesis in several species. However, the function of Ctsl in medaka ovaries remains unclear. Therefore, here, we examined the physiological functions of Ctsl in the medaka ovaries. Ctsl mRNA transcripts and proteins were found to be constitutively expressed in the ovaries of Oryzias latipes over a 24-h spawning cycle. Expression was localized within the oocyte cytoplasm of growing follicles and the follicle layer of preov...
Source: Biology of Reproduction - September 15, 2023 Category: Reproduction Medicine Authors: Yoshiko Kondo Sanath Rajapakse Katsueki Ogiwara Source Type: research
Involvement of cathepsin L in the degradation and degeneration of postovulatory follicle of the medaka ovary
Biol Reprod. 2023 Sep 15:ioad116. doi: 10.1093/biolre/ioad116. Online ahead of print.ABSTRACTCathepsin L (CTSL) plays physiological and pathological roles in immune responses, cancer, metamorphosis, and oogenesis in several species. However, the function of Ctsl in medaka ovaries remains unclear. Therefore, here, we examined the physiological functions of Ctsl in the medaka ovaries. Ctsl mRNA transcripts and proteins were found to be constitutively expressed in the ovaries of Oryzias latipes over a 24-h spawning cycle. Expression was localized within the oocyte cytoplasm of growing follicles and the follicle layer of preov...
Source: Biology of Reproduction - September 15, 2023 Category: Reproduction Medicine Authors: Yoshiko Kondo Sanath Rajapakse Katsueki Ogiwara Source Type: research
Involvement of cathepsin L in the degradation and degeneration of postovulatory follicle of the medaka ovary
Biol Reprod. 2023 Sep 15:ioad116. doi: 10.1093/biolre/ioad116. Online ahead of print.ABSTRACTCathepsin L (CTSL) plays physiological and pathological roles in immune responses, cancer, metamorphosis, and oogenesis in several species. However, the function of Ctsl in medaka ovaries remains unclear. Therefore, here, we examined the physiological functions of Ctsl in the medaka ovaries. Ctsl mRNA transcripts and proteins were found to be constitutively expressed in the ovaries of Oryzias latipes over a 24-h spawning cycle. Expression was localized within the oocyte cytoplasm of growing follicles and the follicle layer of preov...
Source: Biology of Reproduction - September 15, 2023 Category: Reproduction Medicine Authors: Yoshiko Kondo Sanath Rajapakse Katsueki Ogiwara Source Type: research
Involvement of cathepsin L in the degradation and degeneration of postovulatory follicle of the medaka ovary
Biol Reprod. 2023 Sep 15:ioad116. doi: 10.1093/biolre/ioad116. Online ahead of print.ABSTRACTCathepsin L (CTSL) plays physiological and pathological roles in immune responses, cancer, metamorphosis, and oogenesis in several species. However, the function of Ctsl in medaka ovaries remains unclear. Therefore, here, we examined the physiological functions of Ctsl in the medaka ovaries. Ctsl mRNA transcripts and proteins were found to be constitutively expressed in the ovaries of Oryzias latipes over a 24-h spawning cycle. Expression was localized within the oocyte cytoplasm of growing follicles and the follicle layer of preov...
Source: Biology of Reproduction - September 15, 2023 Category: Reproduction Medicine Authors: Yoshiko Kondo Sanath Rajapakse Katsueki Ogiwara Source Type: research
Single cell insights into development of the bovine placenta †
Biol Reprod. 2023 Sep 14:ioad123. doi: 10.1093/biolre/ioad123. Online ahead of print.ABSTRACTA central determinant of pregnancy success is proper development of the conceptus (embryo/fetus and associated extraembryonic membranes including the placenta). Although the gross morphology and histology of the bovine placenta has been well studied, the cellular and molecular mechanisms regulating placenta development and trophoblast differentiation and function remain essentially undefined. Here single cell transcriptome (scRNA-seq) analysis was performed on the day 17 bovine conceptus and chorion of day 24, 30 and 50 conceptuses...
Source: Biology of Reproduction - September 14, 2023 Category: Reproduction Medicine Authors: Kimberly M Davenport Eleanore V O'Neil M Sofia Ortega Amanda Patterson Andrew M Kelleher Wesley C Warren Thomas E Spencer Source Type: research
