The pathogenesis of endometriosis and adenomyosis: insights from single-cell RNA sequencing
Biol Reprod. 2024 Feb 22:ioae032. doi: 10.1093/biolre/ioae032. Online ahead of print.ABSTRACTEndometriosis and adenomyosis are two similar gynecological diseases that are characterized by ectopic implantation and the growth of the endometrial tissue. Previous studies have reported that they share a common pathophysiology in some respects, such as a similar cellular composition and resistance to the progestogen of lesions, but their underlying mechanisms remain elusive. Emerging single-cell RNA sequencing (scRNA-seq) technologies allow for the dissection of single-cell transcriptome mapping to reveal the etiology of disease...
Source: Biology of Reproduction - February 22, 2024 Category: Reproduction Medicine Authors: Qiutong Li Jinghua Shi Dai Yi Xiaoyan Li Zhiyue Gu Hailan Yan Jinhua Leng Source Type: research
The pathogenesis of endometriosis and adenomyosis: insights from single-cell RNA sequencing
Biol Reprod. 2024 Feb 22:ioae032. doi: 10.1093/biolre/ioae032. Online ahead of print.ABSTRACTEndometriosis and adenomyosis are two similar gynecological diseases that are characterized by ectopic implantation and the growth of the endometrial tissue. Previous studies have reported that they share a common pathophysiology in some respects, such as a similar cellular composition and resistance to the progestogen of lesions, but their underlying mechanisms remain elusive. Emerging single-cell RNA sequencing (scRNA-seq) technologies allow for the dissection of single-cell transcriptome mapping to reveal the etiology of disease...
Source: Biology of Reproduction - February 22, 2024 Category: Reproduction Medicine Authors: Qiutong Li Jinghua Shi Dai Yi Xiaoyan Li Zhiyue Gu Hailan Yan Jinhua Leng Source Type: research
Prenatal exposure to DEHP and high-fat diet synergistically disrupts gonadal function in male mice
In this study, we tested the hypothesis that prenatal exposure to a physiologically relevant dose of DEHP in conjunction with HF diet synergistically impacts reproductive function and fertility in male offspring. Female mice were fed a control or HF diet 7 days prior to mating and until their litters were weaned on postnatal day 21. Pregnant dams were exposed to DEHP (20 μg/kg/day) or vehicle from gestational day 10.5 until birth. The male offspring's gross phenotype, sperm quality, serum hormonal levels, testicular histopathology, and testicular gene expression pattern were analyzed. Male mice born to dams exposed to DEH...
Source: Biology of Reproduction - February 21, 2024 Category: Reproduction Medicine Authors: Radwa Barakat Po-Ching Patrick Lin Mary Bunnell Ji-Eun Oh Saniya Rattan Cyrus Arnieri Jodi A Flaws CheMyong J Ko Source Type: research
IGFBP1a is a nutrient deficient response factor that can inhibit fish reproduction through the hypothalamus-pituitary-ovary axis †
Biol Reprod. 2024 Feb 19:ioae009. doi: 10.1093/biolre/ioae009. Online ahead of print.ABSTRACTReproduction is a high energy consuming process, so long-term malnutrition can significantly inhibit gonadal development. However, little is known about the molecular mechanism by which fasting inhibits reproduction. Our present study found that fasting could dramatically induce insulin-like growth factor binding protein 1 (IGFBP1) expression in the liver, hypothalamus, pituitary and ovaries of grass carp. In addition, IGFBP1a in the hypothalamus-pituitary-gonad axis could inhibit the development of gonads. These results indicated ...
Source: Biology of Reproduction - February 20, 2024 Category: Reproduction Medicine Authors: Wei Li Hangyu Li Qiongyao Hu Linlin Wang Zhan Yin Guangfu Hu Source Type: research
Inefficient Sox9 upregulation and absence of Rspo1 repression lead to sex reversal in the B6.XY TIR mouse gonad
Biol Reprod. 2024 Feb 20:ioae018. doi: 10.1093/biolre/ioae018. Online ahead of print.ABSTRACTSry on the Y-chromosome upregulates Sox9, which in turn upregulates a set of genes such as Fgf9 to initiate testicular differentiation in the XY gonad. In the absence of Sry expression, genes such as Rspo1, Foxl2, and Runx1 support ovarian differentiation in the XX gonad. These two pathways antagonize each other to ensure the development of only one gonadal sex in normal development. In the B6.YTIR mouse, carrying the YTIR-chromosome on the B6 genetic background, Sry is expressed in a comparable manner with that in the B6.XY mouse,...
Source: Biology of Reproduction - February 20, 2024 Category: Reproduction Medicine Authors: Jiangqin Cao Fatima El Mansouri Sofia Reynoso Zongping Liu Jiaqiao Zhu Teruko Taketo Source Type: research
IGFBP1a is a nutrient deficient response factor that can inhibit fish reproduction through the hypothalamus-pituitary-ovary axis †
Biol Reprod. 2024 Feb 19:ioae009. doi: 10.1093/biolre/ioae009. Online ahead of print.ABSTRACTReproduction is a high energy consuming process, so long-term malnutrition can significantly inhibit gonadal development. However, little is known about the molecular mechanism by which fasting inhibits reproduction. Our present study found that fasting could dramatically induce insulin-like growth factor binding protein 1 (IGFBP1) expression in the liver, hypothalamus, pituitary and ovaries of grass carp. In addition, IGFBP1a in the hypothalamus-pituitary-gonad axis could inhibit the development of gonads. These results indicated ...
Source: Biology of Reproduction - February 20, 2024 Category: Reproduction Medicine Authors: Wei Li Hangyu Li Qiongyao Hu Linlin Wang Zhan Yin Guangfu Hu Source Type: research
Inefficient Sox9 upregulation and absence of Rspo1 repression lead to sex reversal in the B6.XY TIR mouse gonad
Biol Reprod. 2024 Feb 20:ioae018. doi: 10.1093/biolre/ioae018. Online ahead of print.ABSTRACTSry on the Y-chromosome upregulates Sox9, which in turn upregulates a set of genes such as Fgf9 to initiate testicular differentiation in the XY gonad. In the absence of Sry expression, genes such as Rspo1, Foxl2, and Runx1 support ovarian differentiation in the XX gonad. These two pathways antagonize each other to ensure the development of only one gonadal sex in normal development. In the B6.YTIR mouse, carrying the YTIR-chromosome on the B6 genetic background, Sry is expressed in a comparable manner with that in the B6.XY mouse,...
Source: Biology of Reproduction - February 20, 2024 Category: Reproduction Medicine Authors: Jiangqin Cao Fatima El Mansouri Sofia Reynoso Zongping Liu Jiaqiao Zhu Teruko Taketo Source Type: research
Membrane Inflammasome activation by Choriodecidual Ureaplasma parvum infection without intra-amniotic infection in an NHP model
Biol Reprod. 2024 Feb 9:ioae027. doi: 10.1093/biolre/ioae027. Online ahead of print.ABSTRACTIntrauterine infection is a significant cause of preterm labor and neonatal morbidity and mortality. Ureaplasma parvum is the microorganism most commonly isolated from cases of preterm birth and preterm premature rupture of membranes (pPROM). However, the mechanisms during the early stages of ascending reproductive tract infection remain poorly understood. To examine inflammation in fetal (chorioamnionic) membranes in response to U. parvum infection, we utilized a non-human primate (NHP) model of choriodecidual infection. Eight chro...
Source: Biology of Reproduction - February 9, 2024 Category: Reproduction Medicine Authors: Sudeshna Tripathy Irina Burd Meredith A Kelleher Source Type: research
Membrane Inflammasome activation by Choriodecidual Ureaplasma parvum infection without intra-amniotic infection in an NHP model
Biol Reprod. 2024 Feb 9:ioae027. doi: 10.1093/biolre/ioae027. Online ahead of print.ABSTRACTIntrauterine infection is a significant cause of preterm labor and neonatal morbidity and mortality. Ureaplasma parvum is the microorganism most commonly isolated from cases of preterm birth and preterm premature rupture of membranes (pPROM). However, the mechanisms during the early stages of ascending reproductive tract infection remain poorly understood. To examine inflammation in fetal (chorioamnionic) membranes in response to U. parvum infection, we utilized a non-human primate (NHP) model of choriodecidual infection. Eight chro...
Source: Biology of Reproduction - February 9, 2024 Category: Reproduction Medicine Authors: Sudeshna Tripathy Irina Burd Meredith A Kelleher Source Type: research
Membrane Inflammasome activation by Choriodecidual Ureaplasma parvum infection without intra-amniotic infection in an NHP model
Biol Reprod. 2024 Feb 9:ioae027. doi: 10.1093/biolre/ioae027. Online ahead of print.ABSTRACTIntrauterine infection is a significant cause of preterm labor and neonatal morbidity and mortality. Ureaplasma parvum is the microorganism most commonly isolated from cases of preterm birth and preterm premature rupture of membranes (pPROM). However, the mechanisms during the early stages of ascending reproductive tract infection remain poorly understood. To examine inflammation in fetal (chorioamnionic) membranes in response to U. parvum infection, we utilized a non-human primate (NHP) model of choriodecidual infection. Eight chro...
Source: Biology of Reproduction - February 9, 2024 Category: Reproduction Medicine Authors: Sudeshna Tripathy Irina Burd Meredith A Kelleher Source Type: research
Membrane Inflammasome activation by Choriodecidual Ureaplasma parvum infection without intra-amniotic infection in an NHP model
Biol Reprod. 2024 Feb 9:ioae027. doi: 10.1093/biolre/ioae027. Online ahead of print.ABSTRACTIntrauterine infection is a significant cause of preterm labor and neonatal morbidity and mortality. Ureaplasma parvum is the microorganism most commonly isolated from cases of preterm birth and preterm premature rupture of membranes (pPROM). However, the mechanisms during the early stages of ascending reproductive tract infection remain poorly understood. To examine inflammation in fetal (chorioamnionic) membranes in response to U. parvum infection, we utilized a non-human primate (NHP) model of choriodecidual infection. Eight chro...
Source: Biology of Reproduction - February 9, 2024 Category: Reproduction Medicine Authors: Sudeshna Tripathy Irina Burd Meredith A Kelleher Source Type: research
Membrane Inflammasome activation by Choriodecidual Ureaplasma parvum infection without intra-amniotic infection in an NHP model
Biol Reprod. 2024 Feb 9:ioae027. doi: 10.1093/biolre/ioae027. Online ahead of print.ABSTRACTIntrauterine infection is a significant cause of preterm labor and neonatal morbidity and mortality. Ureaplasma parvum is the microorganism most commonly isolated from cases of preterm birth and preterm premature rupture of membranes (pPROM). However, the mechanisms during the early stages of ascending reproductive tract infection remain poorly understood. To examine inflammation in fetal (chorioamnionic) membranes in response to U. parvum infection, we utilized a non-human primate (NHP) model of choriodecidual infection. Eight chro...
Source: Biology of Reproduction - February 9, 2024 Category: Reproduction Medicine Authors: Sudeshna Tripathy Irina Burd Meredith A Kelleher Source Type: research
Establishment and comparison of human term placenta-derived trophoblast cells
In this study, we describe the development of a human-term placenta-derived cytotrophoblast immortalized cell line (hPTCCTB) derived from the basal plate. hPTCCTB cells are comparable to their primary cells of origin in terms of morphology, marker expression, and functional responses. We demonstrate that these can transform into syncytiotrophoblast and extravillous trophoblasts. We also compared the hPTCCTB cells to immortalized chorionic trophoblasts (hFM-CTC), trophoblasts of the chorionic plate, and BeWo cells, choriocarcinoma cell lines of conventional use. hPTCCTB and hFM-CTCs displayed more similarity to each other t...
Source: Biology of Reproduction - February 8, 2024 Category: Reproduction Medicine Authors: Manuel S Vidal Enkhtuya Radnaa Natasha Vora Kamil Khanipov Cristina Antich Marc Ferrer Rheanna Urrabaz-Garza Jeena E Jacob Ramkumar Menon Source Type: research
Bta-miR-301a targets ACVR1 to influence cleavage time and blastocyst formation rate of early embryos in cattle
In this study, bioinformatics, dual luciferase reporter assays, rescue experiments and gain- and loss-of-function experiments indicated that ACVR1 is the target gene of bta-miR-301a in early bovine embryos. By microinjecting bta-miR-301a mimic into embryos of parthenogenetic or somatic cell nuclear transfer, we observed that bta-miR-301a prolonged the first cleavage time of the embryos and increased the blastocyst formation rate. Thus, this study provides preliminary evidence that bta-miR-301a influences remodeling of the microfilament skeleton, prolongs the first cleavage time, and improves the developmental competence of...
Source: Biology of Reproduction - February 6, 2024 Category: Reproduction Medicine Authors: Heqiang Li Jing Gao Jingyi Wang Baobao Zhao Xiaonan Ma Bihan Zhang Changrong Lv Hailian Qiao Yongsheng Wang Suzhu Qing Source Type: research
Cyclophosphamide activates ferroptosis induced dysfunction of Leydig cells via SMAD2 pathway
In this study, The level of ferroptosis in the mouse model of testicular Leydig cell dysfunction induced by CP was significantly increased and further affected testosterone synthesis. The ferroptosis inhibitors ferrostatin-1and iron chelator deferoxamine can improve injury induced by CP. The results of immunohistochemistry showed that ferrostatin-1 and deferoxamine could improve the structural disorder of seminiferous tubules and the decrease of the number of Leydig cells in testicular tissue induced by CP. Immunofluorescence and western blot confirmed that ferrostatin-1and deferoxamine could improve the expression of key ...
Source: Biology of Reproduction - February 6, 2024 Category: Reproduction Medicine Authors: Senlin Liao Cun Wei Guanyang Wei Haoyu Liang Fan Peng Lei Zhao Ziguang Li Cundong Liu Qizhao Zhou Source Type: research
