LncRNA SNHG11 aggravates cell proliferation and migration in triple-negative breast cancer via sponging miR-2355-5p and targeting CBX5
Exp Ther Med. 2021 Aug;22(2):892. doi: 10.3892/etm.2021.10324. Epub 2021 Jun 18.ABSTRACTTriple-negative breast cancer (TNBC) is one of the most common malignances worldwide. Concurrently, the incidence of TNBC has continued to rise in recent years. It is reported that long non-coding RNAs (lncRNAs) are involved in biological processes in numerous cancers including TNBC. Small nucleolar RNA host gene 11 (SNHG11) has already been studied and reported in some cancers. However, the role of SNHG11 in TNBC remains unknown. RT-qPCR was used to measure gene expression in the current study. CCK-8, colony formation, flow cytometry, ...
Source: Experimental and Therapeutic Medicine - July 14, 2021 Category: General Medicine Authors: Lei Yu Wei Zhang Peng Wang Qi Zhang Aihua Cong Xiuyuan Yang Kai Sang Source Type: research

Sevoflurane exerts improved protective effects than propofol on hypoxia-reoxygenation injury by regulating the microRNA-221-5p/ADAM8 axis in cardiomyocytes
Exp Ther Med. 2021 Aug;22(2):893. doi: 10.3892/etm.2021.10325. Epub 2021 Jun 18.ABSTRACTMyocardial ischemia-reperfusion (I/R) injury is a leading cause of heart disease and death. Decreasing the detrimental effect of I/R remains an urgent issue in clinical practice. The present study examined the interaction of the anesthetics (sevoflurane and propofol), ADAM8, and microRNA (miR)-221-5p in myocardial tissue protection in the hypoxia-reoxygenation (H/R) model. H9C2 cells were cultured and subjected to H/R stimulation for further verifications in vitro. Reverse transcription-quantitative PCR or western blotting was performed...
Source: Experimental and Therapeutic Medicine - July 14, 2021 Category: General Medicine Authors: Dan Xie Huifei Deng Hao Feng Source Type: research

Effect of fibulin-5 on aldosterone-induced apoptosis in human ascending aortic smooth muscle cells
Exp Ther Med. 2021 Aug;22(2):896. doi: 10.3892/etm.2021.10328. Epub 2021 Jun 23.ABSTRACTThe aim of the current study was to investigate the effect of aldosterone on apoptosis in human aortic smooth muscle cells (HA-VSMC) and to determine the role of fibulin-5 in the aldosterone-induced apoptosis of HA-VSMC cells. Through the construction of a fibulin-5 eukaryotic overexpression vector and a short hairpin RNA interference plasmid, fibulin-5 was overexpressed and silenced, respectively. The role of fibulin-5 in the aldosterone-induced apoptosis of HA-VSMC was subsequently determined. The overexpression of fibulin-5 inhibited...
Source: Experimental and Therapeutic Medicine - July 14, 2021 Category: General Medicine Authors: Yongji Yan Xujie Liu Xuanchen Li Xuan Zhou Chaozhi Yang Jintao Tian Jun Pu Xiaoqun Niu Source Type: research

BMP-2 alleviates heart failure with type 2 diabetes mellitus and doxorubicin-induced AC16 cell injury by inhibiting NLRP3 inflammasome-mediated pyroptosis
Exp Ther Med. 2021 Aug;22(2):897. doi: 10.3892/etm.2021.10329. Epub 2021 Jun 23.ABSTRACTChronic heart failure (CHF) and diabetes mellitus are associated with morbidity and mortality. CHF and diabetes generally simultaneously occur, resulting in adverse outcomes. Diabetes complicates cardiomyopathy and exacerbates heart failure conditions. An increase in natriuretic peptides, including atrial natriuretic peptide (ANP), and another endsogenously generated peptide, brain natriuretic peptide (BNP), serves an essential role in CHF. The aim of this study was to explore the molecular regulation between bone morphogenetic protein-...
Source: Experimental and Therapeutic Medicine - July 14, 2021 Category: General Medicine Authors: Jia-Mei Zhang Rui-Qun Yu Feng-Zhu Wu Liang Qiao Xiao-Rong Wu Ying-Jie Fu Yue-Feng Liang Yu Pang Chun-Yi Xie Source Type: research

RNA-binding protein CELF2 inhibits breast cancer cell invasion and angiogenesis by downregulating NFATc1
In conclusion, the findings of the present study demonstrated that CELF2 may inhibit breast cancer cell invasion and angiogenesis by downregulating NFATc1.PMID:34257711 | PMC:PMC8243341 | DOI:10.3892/etm.2021.10330 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - July 14, 2021 Category: General Medicine Authors: Limin Zhou Xiju Xie Source Type: research

Zhike pingchuan granules improve bronchial asthma by regulating the IL-6/JAK2/STAT3 pathway
In conclusion, ZKPC improved bronchial asthma by suppressing the IL-6/JAK2/STAT3 pathway.PMID:34257712 | PMC:PMC8243345 | DOI:10.3892/etm.2021.10331 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - July 14, 2021 Category: General Medicine Authors: Yumei Ren Yuling Liu Shouchuan Wang Zhen Lei Yongbin Yan Xutao Guan Jianghong Hou Shan Zhu Haijun Shan Xinlei Tian Quan Wang Caihong Cao Yingying Zhang Yunfeng Ma Source Type: research

At elevated temperatures, heat shock protein genes show altered ratios of different RNAs and expression of new RNAs, including several novel HSPB1 mRNAs encoding HSP27 protein isoforms
Exp Ther Med. 2021 Aug;22(2):900. doi: 10.3892/etm.2021.10332. Epub 2021 Jun 24.ABSTRACTHeat shock proteins (HSP) serve as chaperones to maintain the physiological conformation and function of numerous cellular proteins when the ambient temperature is increased. To determine how accurate the general assumption that HSP gene expression is increased in febrile situations is, the RNA levels of the HSF1 (heat shock transcription factor 1) gene and certain HSP genes were determined in three cell lines cultured at 37˚C or 39˚C for three days. At 39˚C, the expression of HSF1, HSPB1, HSP90AA1 and HSP70A1L genes demonstrated com...
Source: Experimental and Therapeutic Medicine - July 14, 2021 Category: General Medicine Authors: Xia Gao Keyin Zhang Haiyan Zhou Lucas Zellmer Chengfu Yuan Hai Huang Dezhong Joshua Liao Source Type: research

Clinical impact of copy number variation changes in bladder cancer samples
Exp Ther Med. 2021 Aug;22(2):901. doi: 10.3892/etm.2021.10333. Epub 2021 Jun 24.ABSTRACTThe aim of the present study was to detect copy number variations (CNVs) related to tumour progression and metastasis of urothelial carcinoma through whole-genome scanning. A total of 30 bladder cancer samples staged from pTa to pT4 were included in the study. DNA was extracted from freshly frozen tissue via standard phenol-chloroform extraction and CNV analysis was performed on two alternative platforms (CytoChip Oligo aCGH, 4x44K and Infinium OncoArray-500K BeadChip; Illumina, Inc.). Data were analysed with BlueFuse Multi software and...
Source: Experimental and Therapeutic Medicine - July 14, 2021 Category: General Medicine Authors: Victoria Spasova Boris Mladenov Simeon Rangelov Zora Hammoudeh Desislava Nesheva Dimitar Serbezov Rada Staneva Savina Hadjidekova Mihail Ganev Lubomir Balabanski Radoslava Vazharova Chavdar Slavov Draga Toncheva Olga Antonova Source Type: research

< em > RUNX1 < /em > gene expression changes in the placentas of women smokers
The objective of the current study was to evaluate the impact of exposure to cigarette smoke on the gene expression of STAT1, T-BET, GATA3, IL-4, RUNX1 and RUNX3 during the gestation period, and to determine whether the expression levels of these genes are associated with changes in global methylation. STAT1, GATA3, RUNX1 and RUNX3 protein and mRNA expression levels in the placental tissue of women smokers and non-smoking women were determined via immunohistochemistry and quantitative PCR (qPCR) respectively. Additionally, T-BET and IL-4 mRNA expression levels were determined by qPCR. On the other hand, global methylation ...
Source: Experimental and Therapeutic Medicine - July 14, 2021 Category: General Medicine Authors: Litzy Gisella Bermudez Ithzayana Madariaga Maria Isabel Zu ñiga Mercedes Olaya Alejandra Ca ñas Luz-Stella Rodriguez Olga Maria Moreno Adriana Rojas Source Type: research

Lipoblastoma: Diagnosis and surgical considerations
Exp Ther Med. 2021 Aug;22(2):903. doi: 10.3892/etm.2021.10335. Epub 2021 Jun 24.ABSTRACTLipoblastoma (LB) and lipoblastomatosis (LBS) are uncommon benign mesenchymal tumors of embryonal fat, occurring almost exclusively in infancy and early childhood. These fast-growing tumors have an excellent prognosis if properly treated. Eight consecutive children having pathologically demonstrated LB treated by the same surgical team were retrospectively reviewed. There were 5 boys and 3 girls between 7 to 36 months (median age 22 months). The localization of the tumors was on the thigh (1 case), abdomen (2 cases), axillary and pector...
Source: Experimental and Therapeutic Medicine - July 14, 2021 Category: General Medicine Authors: Radu-Iulian Sp ătaru C ătălin Cîrstoveanu Dan-Alexandru Iozsa Augustina Enculescu Lumini ța Florentina Tomescu Drago ș Șerban Source Type: research

Macrophage activation syndrome: A diagnostic challenge (Review)
Exp Ther Med. 2021 Aug;22(2):904. doi: 10.3892/etm.2021.10336. Epub 2021 Jun 24.ABSTRACTMacrophage activation syndrome (MAS) represents an acute and severe inflammatory syndrome, idiopathic (primary) or secondary to infections, rheumatic diseases, malignancies, or drugs. MAS is underdiagnosed, being confused with sepsis, adverse effects of anti-arthritic drugs or exacerbated symptoms of evolving rheumatologic or infectious diseases. Because of the late diagnosis, most patients do not benefit from effective therapy, leading to death. Elucidation of valid early diagnostic criteria of MAS would be a particularly important ste...
Source: Experimental and Therapeutic Medicine - July 14, 2021 Category: General Medicine Authors: Anca Bojan Andrada Parvu Iulia-Andrea Zsoldos Tunde Torok Anca Daniela Farcas Source Type: research

lncRNA TUG1 regulates human pulmonary microvascular endothelial cell apoptosis via sponging of the miR-9a-5p/BCL2L11 axis in chronic obstructive pulmonary disease
Exp Ther Med. 2021 Aug;22(2):906. doi: 10.3892/etm.2021.10338. Epub 2021 Jun 25.ABSTRACTThe aim of the present study was to investigate the function of long non-coding RNA taurine-upregulated gene 1 (lncRNA TUG1) in chronic obstructive pulmonary disease and further assess the underlying molecular mechanisms. Flow cytometry analysis was performed to detect cell apoptosis of human pulmonary microvascular endothelial cells (HPMECs) treated with 1% cigarette smoke extract (CSE). The activity of caspase-3 was measured using a Caspase-3 Activity assay kit and the protein expression of cleaved caspase-3, caspase-3 and Bcl-2 like ...
Source: Experimental and Therapeutic Medicine - July 14, 2021 Category: General Medicine Authors: Xichen Chen Mao Mao Ying Shen Xueyong Jiang Zhifei Yin Source Type: research

First Chinese patient with mental retardation-40 due to a < em > de novo < /em > CHAMP1 frameshift mutation: Case report and literature review
Exp Ther Med. 2021 Aug;22(2):907. doi: 10.3892/etm.2021.10339. Epub 2021 Jun 25.ABSTRACTMental retardation-40 (MRD40) is a rare autosomal dominant neurodevelopmental disorder with a poor prognosis that is caused by a heterozygous mutation in chromosome alignment maintaining phosphoprotein 1 (CHAMP1). It was previously considered a non-syndromic disease due to the lack of specific external features. Only limited international reports describing CHAMP1 mutations are currently available. The present case study was the first to report on a Chinese patient with MRD40. The patient presented with severe global development delay w...
Source: Experimental and Therapeutic Medicine - July 14, 2021 Category: General Medicine Authors: Yan Dong Xiaoyi Shi Kaixian Du Ruijuan Xu Tianming Jia Jun Wang Lijun Wang Rui Han Source Type: research

Cannabinoid receptor 2 deficiency enhances isoflurane-induced spatial cognitive impairment in adult mice by affecting neuroinflammation, neurogenesis and neuroplasticity
Exp Ther Med. 2021 Aug;22(2):908. doi: 10.3892/etm.2021.10340. Epub 2021 Jun 25.ABSTRACTIsoflurane (Iso) is a commonly used inhalational anesthetic and is associated with the incidence of postoperative cognitive dysfunction (POCD). Cannabinoid receptor 2 (CB2R) was previously reported to have a promising neuroprotective function in cases of POCD, but the specific mechanisms have remained to be fully explored. The aim of the present study was to investigate the effect of CB2R deficiency on spatial cognitive performance in adult mice exposed to Iso. A total of 20 adult CB2R knockout (KO) and 20 wild-type (WT) mice were expos...
Source: Experimental and Therapeutic Medicine - July 14, 2021 Category: General Medicine Authors: Chao Li Jingpu Shi Jiaguang Sun Yuanyuan Shi Huiqun Jia Source Type: research

LncRNA SNHG11 aggravates cell proliferation and migration in triple-negative breast cancer via sponging miR-2355-5p and targeting CBX5
Exp Ther Med. 2021 Aug;22(2):892. doi: 10.3892/etm.2021.10324. Epub 2021 Jun 18.ABSTRACTTriple-negative breast cancer (TNBC) is one of the most common malignances worldwide. Concurrently, the incidence of TNBC has continued to rise in recent years. It is reported that long non-coding RNAs (lncRNAs) are involved in biological processes in numerous cancers including TNBC. Small nucleolar RNA host gene 11 (SNHG11) has already been studied and reported in some cancers. However, the role of SNHG11 in TNBC remains unknown. RT-qPCR was used to measure gene expression in the current study. CCK-8, colony formation, flow cytometry, ...
Source: Experimental and Therapeutic Medicine - July 14, 2021 Category: General Medicine Authors: Lei Yu Wei Zhang Peng Wang Qi Zhang Aihua Cong Xiuyuan Yang Kai Sang Source Type: research

Sevoflurane exerts improved protective effects than propofol on hypoxia-reoxygenation injury by regulating the microRNA-221-5p/ADAM8 axis in cardiomyocytes
Exp Ther Med. 2021 Aug;22(2):893. doi: 10.3892/etm.2021.10325. Epub 2021 Jun 18.ABSTRACTMyocardial ischemia-reperfusion (I/R) injury is a leading cause of heart disease and death. Decreasing the detrimental effect of I/R remains an urgent issue in clinical practice. The present study examined the interaction of the anesthetics (sevoflurane and propofol), ADAM8, and microRNA (miR)-221-5p in myocardial tissue protection in the hypoxia-reoxygenation (H/R) model. H9C2 cells were cultured and subjected to H/R stimulation for further verifications in vitro. Reverse transcription-quantitative PCR or western blotting was performed...
Source: Experimental and Therapeutic Medicine - July 14, 2021 Category: General Medicine Authors: Dan Xie Huifei Deng Hao Feng Source Type: research

Effect of fibulin-5 on aldosterone-induced apoptosis in human ascending aortic smooth muscle cells
Exp Ther Med. 2021 Aug;22(2):896. doi: 10.3892/etm.2021.10328. Epub 2021 Jun 23.ABSTRACTThe aim of the current study was to investigate the effect of aldosterone on apoptosis in human aortic smooth muscle cells (HA-VSMC) and to determine the role of fibulin-5 in the aldosterone-induced apoptosis of HA-VSMC cells. Through the construction of a fibulin-5 eukaryotic overexpression vector and a short hairpin RNA interference plasmid, fibulin-5 was overexpressed and silenced, respectively. The role of fibulin-5 in the aldosterone-induced apoptosis of HA-VSMC was subsequently determined. The overexpression of fibulin-5 inhibited...
Source: Experimental and Therapeutic Medicine - July 14, 2021 Category: General Medicine Authors: Yongji Yan Xujie Liu Xuanchen Li Xuan Zhou Chaozhi Yang Jintao Tian Jun Pu Xiaoqun Niu Source Type: research

BMP-2 alleviates heart failure with type 2 diabetes mellitus and doxorubicin-induced AC16 cell injury by inhibiting NLRP3 inflammasome-mediated pyroptosis
Exp Ther Med. 2021 Aug;22(2):897. doi: 10.3892/etm.2021.10329. Epub 2021 Jun 23.ABSTRACTChronic heart failure (CHF) and diabetes mellitus are associated with morbidity and mortality. CHF and diabetes generally simultaneously occur, resulting in adverse outcomes. Diabetes complicates cardiomyopathy and exacerbates heart failure conditions. An increase in natriuretic peptides, including atrial natriuretic peptide (ANP), and another endsogenously generated peptide, brain natriuretic peptide (BNP), serves an essential role in CHF. The aim of this study was to explore the molecular regulation between bone morphogenetic protein-...
Source: Experimental and Therapeutic Medicine - July 14, 2021 Category: General Medicine Authors: Jia-Mei Zhang Rui-Qun Yu Feng-Zhu Wu Liang Qiao Xiao-Rong Wu Ying-Jie Fu Yue-Feng Liang Yu Pang Chun-Yi Xie Source Type: research

RNA-binding protein CELF2 inhibits breast cancer cell invasion and angiogenesis by downregulating NFATc1
In conclusion, the findings of the present study demonstrated that CELF2 may inhibit breast cancer cell invasion and angiogenesis by downregulating NFATc1.PMID:34257711 | PMC:PMC8243341 | DOI:10.3892/etm.2021.10330 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - July 14, 2021 Category: General Medicine Authors: Limin Zhou Xiju Xie Source Type: research

Zhike pingchuan granules improve bronchial asthma by regulating the IL-6/JAK2/STAT3 pathway
In conclusion, ZKPC improved bronchial asthma by suppressing the IL-6/JAK2/STAT3 pathway.PMID:34257712 | PMC:PMC8243345 | DOI:10.3892/etm.2021.10331 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - July 14, 2021 Category: General Medicine Authors: Yumei Ren Yuling Liu Shouchuan Wang Zhen Lei Yongbin Yan Xutao Guan Jianghong Hou Shan Zhu Haijun Shan Xinlei Tian Quan Wang Caihong Cao Yingying Zhang Yunfeng Ma Source Type: research

At elevated temperatures, heat shock protein genes show altered ratios of different RNAs and expression of new RNAs, including several novel HSPB1 mRNAs encoding HSP27 protein isoforms
Exp Ther Med. 2021 Aug;22(2):900. doi: 10.3892/etm.2021.10332. Epub 2021 Jun 24.ABSTRACTHeat shock proteins (HSP) serve as chaperones to maintain the physiological conformation and function of numerous cellular proteins when the ambient temperature is increased. To determine how accurate the general assumption that HSP gene expression is increased in febrile situations is, the RNA levels of the HSF1 (heat shock transcription factor 1) gene and certain HSP genes were determined in three cell lines cultured at 37˚C or 39˚C for three days. At 39˚C, the expression of HSF1, HSPB1, HSP90AA1 and HSP70A1L genes demonstrated com...
Source: Experimental and Therapeutic Medicine - July 14, 2021 Category: General Medicine Authors: Xia Gao Keyin Zhang Haiyan Zhou Lucas Zellmer Chengfu Yuan Hai Huang Dezhong Joshua Liao Source Type: research

Clinical impact of copy number variation changes in bladder cancer samples
Exp Ther Med. 2021 Aug;22(2):901. doi: 10.3892/etm.2021.10333. Epub 2021 Jun 24.ABSTRACTThe aim of the present study was to detect copy number variations (CNVs) related to tumour progression and metastasis of urothelial carcinoma through whole-genome scanning. A total of 30 bladder cancer samples staged from pTa to pT4 were included in the study. DNA was extracted from freshly frozen tissue via standard phenol-chloroform extraction and CNV analysis was performed on two alternative platforms (CytoChip Oligo aCGH, 4x44K and Infinium OncoArray-500K BeadChip; Illumina, Inc.). Data were analysed with BlueFuse Multi software and...
Source: Experimental and Therapeutic Medicine - July 14, 2021 Category: General Medicine Authors: Victoria Spasova Boris Mladenov Simeon Rangelov Zora Hammoudeh Desislava Nesheva Dimitar Serbezov Rada Staneva Savina Hadjidekova Mihail Ganev Lubomir Balabanski Radoslava Vazharova Chavdar Slavov Draga Toncheva Olga Antonova Source Type: research

< em > RUNX1 < /em > gene expression changes in the placentas of women smokers
The objective of the current study was to evaluate the impact of exposure to cigarette smoke on the gene expression of STAT1, T-BET, GATA3, IL-4, RUNX1 and RUNX3 during the gestation period, and to determine whether the expression levels of these genes are associated with changes in global methylation. STAT1, GATA3, RUNX1 and RUNX3 protein and mRNA expression levels in the placental tissue of women smokers and non-smoking women were determined via immunohistochemistry and quantitative PCR (qPCR) respectively. Additionally, T-BET and IL-4 mRNA expression levels were determined by qPCR. On the other hand, global methylation ...
Source: Experimental and Therapeutic Medicine - July 14, 2021 Category: General Medicine Authors: Litzy Gisella Bermudez Ithzayana Madariaga Maria Isabel Zu ñiga Mercedes Olaya Alejandra Ca ñas Luz-Stella Rodriguez Olga Maria Moreno Adriana Rojas Source Type: research

Lipoblastoma: Diagnosis and surgical considerations
Exp Ther Med. 2021 Aug;22(2):903. doi: 10.3892/etm.2021.10335. Epub 2021 Jun 24.ABSTRACTLipoblastoma (LB) and lipoblastomatosis (LBS) are uncommon benign mesenchymal tumors of embryonal fat, occurring almost exclusively in infancy and early childhood. These fast-growing tumors have an excellent prognosis if properly treated. Eight consecutive children having pathologically demonstrated LB treated by the same surgical team were retrospectively reviewed. There were 5 boys and 3 girls between 7 to 36 months (median age 22 months). The localization of the tumors was on the thigh (1 case), abdomen (2 cases), axillary and pector...
Source: Experimental and Therapeutic Medicine - July 14, 2021 Category: General Medicine Authors: Radu-Iulian Sp ătaru C ătălin Cîrstoveanu Dan-Alexandru Iozsa Augustina Enculescu Lumini ța Florentina Tomescu Drago ș Șerban Source Type: research

Macrophage activation syndrome: A diagnostic challenge (Review)
Exp Ther Med. 2021 Aug;22(2):904. doi: 10.3892/etm.2021.10336. Epub 2021 Jun 24.ABSTRACTMacrophage activation syndrome (MAS) represents an acute and severe inflammatory syndrome, idiopathic (primary) or secondary to infections, rheumatic diseases, malignancies, or drugs. MAS is underdiagnosed, being confused with sepsis, adverse effects of anti-arthritic drugs or exacerbated symptoms of evolving rheumatologic or infectious diseases. Because of the late diagnosis, most patients do not benefit from effective therapy, leading to death. Elucidation of valid early diagnostic criteria of MAS would be a particularly important ste...
Source: Experimental and Therapeutic Medicine - July 14, 2021 Category: General Medicine Authors: Anca Bojan Andrada Parvu Iulia-Andrea Zsoldos Tunde Torok Anca Daniela Farcas Source Type: research

lncRNA TUG1 regulates human pulmonary microvascular endothelial cell apoptosis via sponging of the miR-9a-5p/BCL2L11 axis in chronic obstructive pulmonary disease
Exp Ther Med. 2021 Aug;22(2):906. doi: 10.3892/etm.2021.10338. Epub 2021 Jun 25.ABSTRACTThe aim of the present study was to investigate the function of long non-coding RNA taurine-upregulated gene 1 (lncRNA TUG1) in chronic obstructive pulmonary disease and further assess the underlying molecular mechanisms. Flow cytometry analysis was performed to detect cell apoptosis of human pulmonary microvascular endothelial cells (HPMECs) treated with 1% cigarette smoke extract (CSE). The activity of caspase-3 was measured using a Caspase-3 Activity assay kit and the protein expression of cleaved caspase-3, caspase-3 and Bcl-2 like ...
Source: Experimental and Therapeutic Medicine - July 14, 2021 Category: General Medicine Authors: Xichen Chen Mao Mao Ying Shen Xueyong Jiang Zhifei Yin Source Type: research

First Chinese patient with mental retardation-40 due to a < em > de novo < /em > CHAMP1 frameshift mutation: Case report and literature review
Exp Ther Med. 2021 Aug;22(2):907. doi: 10.3892/etm.2021.10339. Epub 2021 Jun 25.ABSTRACTMental retardation-40 (MRD40) is a rare autosomal dominant neurodevelopmental disorder with a poor prognosis that is caused by a heterozygous mutation in chromosome alignment maintaining phosphoprotein 1 (CHAMP1). It was previously considered a non-syndromic disease due to the lack of specific external features. Only limited international reports describing CHAMP1 mutations are currently available. The present case study was the first to report on a Chinese patient with MRD40. The patient presented with severe global development delay w...
Source: Experimental and Therapeutic Medicine - July 14, 2021 Category: General Medicine Authors: Yan Dong Xiaoyi Shi Kaixian Du Ruijuan Xu Tianming Jia Jun Wang Lijun Wang Rui Han Source Type: research

Cannabinoid receptor 2 deficiency enhances isoflurane-induced spatial cognitive impairment in adult mice by affecting neuroinflammation, neurogenesis and neuroplasticity
Exp Ther Med. 2021 Aug;22(2):908. doi: 10.3892/etm.2021.10340. Epub 2021 Jun 25.ABSTRACTIsoflurane (Iso) is a commonly used inhalational anesthetic and is associated with the incidence of postoperative cognitive dysfunction (POCD). Cannabinoid receptor 2 (CB2R) was previously reported to have a promising neuroprotective function in cases of POCD, but the specific mechanisms have remained to be fully explored. The aim of the present study was to investigate the effect of CB2R deficiency on spatial cognitive performance in adult mice exposed to Iso. A total of 20 adult CB2R knockout (KO) and 20 wild-type (WT) mice were expos...
Source: Experimental and Therapeutic Medicine - July 14, 2021 Category: General Medicine Authors: Chao Li Jingpu Shi Jiaguang Sun Yuanyuan Shi Huiqun Jia Source Type: research

LncRNA SNHG11 aggravates cell proliferation and migration in triple-negative breast cancer via sponging miR-2355-5p and targeting CBX5
Exp Ther Med. 2021 Aug;22(2):892. doi: 10.3892/etm.2021.10324. Epub 2021 Jun 18.ABSTRACTTriple-negative breast cancer (TNBC) is one of the most common malignances worldwide. Concurrently, the incidence of TNBC has continued to rise in recent years. It is reported that long non-coding RNAs (lncRNAs) are involved in biological processes in numerous cancers including TNBC. Small nucleolar RNA host gene 11 (SNHG11) has already been studied and reported in some cancers. However, the role of SNHG11 in TNBC remains unknown. RT-qPCR was used to measure gene expression in the current study. CCK-8, colony formation, flow cytometry, ...
Source: Experimental and Therapeutic Medicine - July 14, 2021 Category: General Medicine Authors: Lei Yu Wei Zhang Peng Wang Qi Zhang Aihua Cong Xiuyuan Yang Kai Sang Source Type: research

Sevoflurane exerts improved protective effects than propofol on hypoxia-reoxygenation injury by regulating the microRNA-221-5p/ADAM8 axis in cardiomyocytes
Exp Ther Med. 2021 Aug;22(2):893. doi: 10.3892/etm.2021.10325. Epub 2021 Jun 18.ABSTRACTMyocardial ischemia-reperfusion (I/R) injury is a leading cause of heart disease and death. Decreasing the detrimental effect of I/R remains an urgent issue in clinical practice. The present study examined the interaction of the anesthetics (sevoflurane and propofol), ADAM8, and microRNA (miR)-221-5p in myocardial tissue protection in the hypoxia-reoxygenation (H/R) model. H9C2 cells were cultured and subjected to H/R stimulation for further verifications in vitro. Reverse transcription-quantitative PCR or western blotting was performed...
Source: Experimental and Therapeutic Medicine - July 14, 2021 Category: General Medicine Authors: Dan Xie Huifei Deng Hao Feng Source Type: research

Effect of fibulin-5 on aldosterone-induced apoptosis in human ascending aortic smooth muscle cells
Exp Ther Med. 2021 Aug;22(2):896. doi: 10.3892/etm.2021.10328. Epub 2021 Jun 23.ABSTRACTThe aim of the current study was to investigate the effect of aldosterone on apoptosis in human aortic smooth muscle cells (HA-VSMC) and to determine the role of fibulin-5 in the aldosterone-induced apoptosis of HA-VSMC cells. Through the construction of a fibulin-5 eukaryotic overexpression vector and a short hairpin RNA interference plasmid, fibulin-5 was overexpressed and silenced, respectively. The role of fibulin-5 in the aldosterone-induced apoptosis of HA-VSMC was subsequently determined. The overexpression of fibulin-5 inhibited...
Source: Experimental and Therapeutic Medicine - July 14, 2021 Category: General Medicine Authors: Yongji Yan Xujie Liu Xuanchen Li Xuan Zhou Chaozhi Yang Jintao Tian Jun Pu Xiaoqun Niu Source Type: research

BMP-2 alleviates heart failure with type 2 diabetes mellitus and doxorubicin-induced AC16 cell injury by inhibiting NLRP3 inflammasome-mediated pyroptosis
Exp Ther Med. 2021 Aug;22(2):897. doi: 10.3892/etm.2021.10329. Epub 2021 Jun 23.ABSTRACTChronic heart failure (CHF) and diabetes mellitus are associated with morbidity and mortality. CHF and diabetes generally simultaneously occur, resulting in adverse outcomes. Diabetes complicates cardiomyopathy and exacerbates heart failure conditions. An increase in natriuretic peptides, including atrial natriuretic peptide (ANP), and another endsogenously generated peptide, brain natriuretic peptide (BNP), serves an essential role in CHF. The aim of this study was to explore the molecular regulation between bone morphogenetic protein-...
Source: Experimental and Therapeutic Medicine - July 14, 2021 Category: General Medicine Authors: Jia-Mei Zhang Rui-Qun Yu Feng-Zhu Wu Liang Qiao Xiao-Rong Wu Ying-Jie Fu Yue-Feng Liang Yu Pang Chun-Yi Xie Source Type: research

RNA-binding protein CELF2 inhibits breast cancer cell invasion and angiogenesis by downregulating NFATc1
In conclusion, the findings of the present study demonstrated that CELF2 may inhibit breast cancer cell invasion and angiogenesis by downregulating NFATc1.PMID:34257711 | PMC:PMC8243341 | DOI:10.3892/etm.2021.10330 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - July 14, 2021 Category: General Medicine Authors: Limin Zhou Xiju Xie Source Type: research

Zhike pingchuan granules improve bronchial asthma by regulating the IL-6/JAK2/STAT3 pathway
In conclusion, ZKPC improved bronchial asthma by suppressing the IL-6/JAK2/STAT3 pathway.PMID:34257712 | PMC:PMC8243345 | DOI:10.3892/etm.2021.10331 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - July 14, 2021 Category: General Medicine Authors: Yumei Ren Yuling Liu Shouchuan Wang Zhen Lei Yongbin Yan Xutao Guan Jianghong Hou Shan Zhu Haijun Shan Xinlei Tian Quan Wang Caihong Cao Yingying Zhang Yunfeng Ma Source Type: research

At elevated temperatures, heat shock protein genes show altered ratios of different RNAs and expression of new RNAs, including several novel HSPB1 mRNAs encoding HSP27 protein isoforms
Exp Ther Med. 2021 Aug;22(2):900. doi: 10.3892/etm.2021.10332. Epub 2021 Jun 24.ABSTRACTHeat shock proteins (HSP) serve as chaperones to maintain the physiological conformation and function of numerous cellular proteins when the ambient temperature is increased. To determine how accurate the general assumption that HSP gene expression is increased in febrile situations is, the RNA levels of the HSF1 (heat shock transcription factor 1) gene and certain HSP genes were determined in three cell lines cultured at 37˚C or 39˚C for three days. At 39˚C, the expression of HSF1, HSPB1, HSP90AA1 and HSP70A1L genes demonstrated com...
Source: Experimental and Therapeutic Medicine - July 14, 2021 Category: General Medicine Authors: Xia Gao Keyin Zhang Haiyan Zhou Lucas Zellmer Chengfu Yuan Hai Huang Dezhong Joshua Liao Source Type: research

Clinical impact of copy number variation changes in bladder cancer samples
Exp Ther Med. 2021 Aug;22(2):901. doi: 10.3892/etm.2021.10333. Epub 2021 Jun 24.ABSTRACTThe aim of the present study was to detect copy number variations (CNVs) related to tumour progression and metastasis of urothelial carcinoma through whole-genome scanning. A total of 30 bladder cancer samples staged from pTa to pT4 were included in the study. DNA was extracted from freshly frozen tissue via standard phenol-chloroform extraction and CNV analysis was performed on two alternative platforms (CytoChip Oligo aCGH, 4x44K and Infinium OncoArray-500K BeadChip; Illumina, Inc.). Data were analysed with BlueFuse Multi software and...
Source: Experimental and Therapeutic Medicine - July 14, 2021 Category: General Medicine Authors: Victoria Spasova Boris Mladenov Simeon Rangelov Zora Hammoudeh Desislava Nesheva Dimitar Serbezov Rada Staneva Savina Hadjidekova Mihail Ganev Lubomir Balabanski Radoslava Vazharova Chavdar Slavov Draga Toncheva Olga Antonova Source Type: research

< em > RUNX1 < /em > gene expression changes in the placentas of women smokers
The objective of the current study was to evaluate the impact of exposure to cigarette smoke on the gene expression of STAT1, T-BET, GATA3, IL-4, RUNX1 and RUNX3 during the gestation period, and to determine whether the expression levels of these genes are associated with changes in global methylation. STAT1, GATA3, RUNX1 and RUNX3 protein and mRNA expression levels in the placental tissue of women smokers and non-smoking women were determined via immunohistochemistry and quantitative PCR (qPCR) respectively. Additionally, T-BET and IL-4 mRNA expression levels were determined by qPCR. On the other hand, global methylation ...
Source: Experimental and Therapeutic Medicine - July 14, 2021 Category: General Medicine Authors: Litzy Gisella Bermudez Ithzayana Madariaga Maria Isabel Zu ñiga Mercedes Olaya Alejandra Ca ñas Luz-Stella Rodriguez Olga Maria Moreno Adriana Rojas Source Type: research

Lipoblastoma: Diagnosis and surgical considerations
Exp Ther Med. 2021 Aug;22(2):903. doi: 10.3892/etm.2021.10335. Epub 2021 Jun 24.ABSTRACTLipoblastoma (LB) and lipoblastomatosis (LBS) are uncommon benign mesenchymal tumors of embryonal fat, occurring almost exclusively in infancy and early childhood. These fast-growing tumors have an excellent prognosis if properly treated. Eight consecutive children having pathologically demonstrated LB treated by the same surgical team were retrospectively reviewed. There were 5 boys and 3 girls between 7 to 36 months (median age 22 months). The localization of the tumors was on the thigh (1 case), abdomen (2 cases), axillary and pector...
Source: Experimental and Therapeutic Medicine - July 14, 2021 Category: General Medicine Authors: Radu-Iulian Sp ătaru C ătălin Cîrstoveanu Dan-Alexandru Iozsa Augustina Enculescu Lumini ța Florentina Tomescu Drago ș Șerban Source Type: research

Macrophage activation syndrome: A diagnostic challenge (Review)
Exp Ther Med. 2021 Aug;22(2):904. doi: 10.3892/etm.2021.10336. Epub 2021 Jun 24.ABSTRACTMacrophage activation syndrome (MAS) represents an acute and severe inflammatory syndrome, idiopathic (primary) or secondary to infections, rheumatic diseases, malignancies, or drugs. MAS is underdiagnosed, being confused with sepsis, adverse effects of anti-arthritic drugs or exacerbated symptoms of evolving rheumatologic or infectious diseases. Because of the late diagnosis, most patients do not benefit from effective therapy, leading to death. Elucidation of valid early diagnostic criteria of MAS would be a particularly important ste...
Source: Experimental and Therapeutic Medicine - July 14, 2021 Category: General Medicine Authors: Anca Bojan Andrada Parvu Iulia-Andrea Zsoldos Tunde Torok Anca Daniela Farcas Source Type: research

lncRNA TUG1 regulates human pulmonary microvascular endothelial cell apoptosis via sponging of the miR-9a-5p/BCL2L11 axis in chronic obstructive pulmonary disease
Exp Ther Med. 2021 Aug;22(2):906. doi: 10.3892/etm.2021.10338. Epub 2021 Jun 25.ABSTRACTThe aim of the present study was to investigate the function of long non-coding RNA taurine-upregulated gene 1 (lncRNA TUG1) in chronic obstructive pulmonary disease and further assess the underlying molecular mechanisms. Flow cytometry analysis was performed to detect cell apoptosis of human pulmonary microvascular endothelial cells (HPMECs) treated with 1% cigarette smoke extract (CSE). The activity of caspase-3 was measured using a Caspase-3 Activity assay kit and the protein expression of cleaved caspase-3, caspase-3 and Bcl-2 like ...
Source: Experimental and Therapeutic Medicine - July 14, 2021 Category: General Medicine Authors: Xichen Chen Mao Mao Ying Shen Xueyong Jiang Zhifei Yin Source Type: research

First Chinese patient with mental retardation-40 due to a < em > de novo < /em > CHAMP1 frameshift mutation: Case report and literature review
Exp Ther Med. 2021 Aug;22(2):907. doi: 10.3892/etm.2021.10339. Epub 2021 Jun 25.ABSTRACTMental retardation-40 (MRD40) is a rare autosomal dominant neurodevelopmental disorder with a poor prognosis that is caused by a heterozygous mutation in chromosome alignment maintaining phosphoprotein 1 (CHAMP1). It was previously considered a non-syndromic disease due to the lack of specific external features. Only limited international reports describing CHAMP1 mutations are currently available. The present case study was the first to report on a Chinese patient with MRD40. The patient presented with severe global development delay w...
Source: Experimental and Therapeutic Medicine - July 14, 2021 Category: General Medicine Authors: Yan Dong Xiaoyi Shi Kaixian Du Ruijuan Xu Tianming Jia Jun Wang Lijun Wang Rui Han Source Type: research

Cannabinoid receptor 2 deficiency enhances isoflurane-induced spatial cognitive impairment in adult mice by affecting neuroinflammation, neurogenesis and neuroplasticity
Exp Ther Med. 2021 Aug;22(2):908. doi: 10.3892/etm.2021.10340. Epub 2021 Jun 25.ABSTRACTIsoflurane (Iso) is a commonly used inhalational anesthetic and is associated with the incidence of postoperative cognitive dysfunction (POCD). Cannabinoid receptor 2 (CB2R) was previously reported to have a promising neuroprotective function in cases of POCD, but the specific mechanisms have remained to be fully explored. The aim of the present study was to investigate the effect of CB2R deficiency on spatial cognitive performance in adult mice exposed to Iso. A total of 20 adult CB2R knockout (KO) and 20 wild-type (WT) mice were expos...
Source: Experimental and Therapeutic Medicine - July 14, 2021 Category: General Medicine Authors: Chao Li Jingpu Shi Jiaguang Sun Yuanyuan Shi Huiqun Jia Source Type: research

LncRNA SNHG11 aggravates cell proliferation and migration in triple-negative breast cancer via sponging miR-2355-5p and targeting CBX5
Exp Ther Med. 2021 Aug;22(2):892. doi: 10.3892/etm.2021.10324. Epub 2021 Jun 18.ABSTRACTTriple-negative breast cancer (TNBC) is one of the most common malignances worldwide. Concurrently, the incidence of TNBC has continued to rise in recent years. It is reported that long non-coding RNAs (lncRNAs) are involved in biological processes in numerous cancers including TNBC. Small nucleolar RNA host gene 11 (SNHG11) has already been studied and reported in some cancers. However, the role of SNHG11 in TNBC remains unknown. RT-qPCR was used to measure gene expression in the current study. CCK-8, colony formation, flow cytometry, ...
Source: Experimental and Therapeutic Medicine - July 14, 2021 Category: General Medicine Authors: Lei Yu Wei Zhang Peng Wang Qi Zhang Aihua Cong Xiuyuan Yang Kai Sang Source Type: research

Sevoflurane exerts improved protective effects than propofol on hypoxia-reoxygenation injury by regulating the microRNA-221-5p/ADAM8 axis in cardiomyocytes
Exp Ther Med. 2021 Aug;22(2):893. doi: 10.3892/etm.2021.10325. Epub 2021 Jun 18.ABSTRACTMyocardial ischemia-reperfusion (I/R) injury is a leading cause of heart disease and death. Decreasing the detrimental effect of I/R remains an urgent issue in clinical practice. The present study examined the interaction of the anesthetics (sevoflurane and propofol), ADAM8, and microRNA (miR)-221-5p in myocardial tissue protection in the hypoxia-reoxygenation (H/R) model. H9C2 cells were cultured and subjected to H/R stimulation for further verifications in vitro. Reverse transcription-quantitative PCR or western blotting was performed...
Source: Experimental and Therapeutic Medicine - July 14, 2021 Category: General Medicine Authors: Dan Xie Huifei Deng Hao Feng Source Type: research

Effect of fibulin-5 on aldosterone-induced apoptosis in human ascending aortic smooth muscle cells
Exp Ther Med. 2021 Aug;22(2):896. doi: 10.3892/etm.2021.10328. Epub 2021 Jun 23.ABSTRACTThe aim of the current study was to investigate the effect of aldosterone on apoptosis in human aortic smooth muscle cells (HA-VSMC) and to determine the role of fibulin-5 in the aldosterone-induced apoptosis of HA-VSMC cells. Through the construction of a fibulin-5 eukaryotic overexpression vector and a short hairpin RNA interference plasmid, fibulin-5 was overexpressed and silenced, respectively. The role of fibulin-5 in the aldosterone-induced apoptosis of HA-VSMC was subsequently determined. The overexpression of fibulin-5 inhibited...
Source: Experimental and Therapeutic Medicine - July 14, 2021 Category: General Medicine Authors: Yongji Yan Xujie Liu Xuanchen Li Xuan Zhou Chaozhi Yang Jintao Tian Jun Pu Xiaoqun Niu Source Type: research

BMP-2 alleviates heart failure with type 2 diabetes mellitus and doxorubicin-induced AC16 cell injury by inhibiting NLRP3 inflammasome-mediated pyroptosis
Exp Ther Med. 2021 Aug;22(2):897. doi: 10.3892/etm.2021.10329. Epub 2021 Jun 23.ABSTRACTChronic heart failure (CHF) and diabetes mellitus are associated with morbidity and mortality. CHF and diabetes generally simultaneously occur, resulting in adverse outcomes. Diabetes complicates cardiomyopathy and exacerbates heart failure conditions. An increase in natriuretic peptides, including atrial natriuretic peptide (ANP), and another endsogenously generated peptide, brain natriuretic peptide (BNP), serves an essential role in CHF. The aim of this study was to explore the molecular regulation between bone morphogenetic protein-...
Source: Experimental and Therapeutic Medicine - July 14, 2021 Category: General Medicine Authors: Jia-Mei Zhang Rui-Qun Yu Feng-Zhu Wu Liang Qiao Xiao-Rong Wu Ying-Jie Fu Yue-Feng Liang Yu Pang Chun-Yi Xie Source Type: research

RNA-binding protein CELF2 inhibits breast cancer cell invasion and angiogenesis by downregulating NFATc1
In conclusion, the findings of the present study demonstrated that CELF2 may inhibit breast cancer cell invasion and angiogenesis by downregulating NFATc1.PMID:34257711 | PMC:PMC8243341 | DOI:10.3892/etm.2021.10330 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - July 14, 2021 Category: General Medicine Authors: Limin Zhou Xiju Xie Source Type: research

Zhike pingchuan granules improve bronchial asthma by regulating the IL-6/JAK2/STAT3 pathway
In conclusion, ZKPC improved bronchial asthma by suppressing the IL-6/JAK2/STAT3 pathway.PMID:34257712 | PMC:PMC8243345 | DOI:10.3892/etm.2021.10331 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - July 14, 2021 Category: General Medicine Authors: Yumei Ren Yuling Liu Shouchuan Wang Zhen Lei Yongbin Yan Xutao Guan Jianghong Hou Shan Zhu Haijun Shan Xinlei Tian Quan Wang Caihong Cao Yingying Zhang Yunfeng Ma Source Type: research

At elevated temperatures, heat shock protein genes show altered ratios of different RNAs and expression of new RNAs, including several novel HSPB1 mRNAs encoding HSP27 protein isoforms
Exp Ther Med. 2021 Aug;22(2):900. doi: 10.3892/etm.2021.10332. Epub 2021 Jun 24.ABSTRACTHeat shock proteins (HSP) serve as chaperones to maintain the physiological conformation and function of numerous cellular proteins when the ambient temperature is increased. To determine how accurate the general assumption that HSP gene expression is increased in febrile situations is, the RNA levels of the HSF1 (heat shock transcription factor 1) gene and certain HSP genes were determined in three cell lines cultured at 37˚C or 39˚C for three days. At 39˚C, the expression of HSF1, HSPB1, HSP90AA1 and HSP70A1L genes demonstrated com...
Source: Experimental and Therapeutic Medicine - July 14, 2021 Category: General Medicine Authors: Xia Gao Keyin Zhang Haiyan Zhou Lucas Zellmer Chengfu Yuan Hai Huang Dezhong Joshua Liao Source Type: research

Clinical impact of copy number variation changes in bladder cancer samples
Exp Ther Med. 2021 Aug;22(2):901. doi: 10.3892/etm.2021.10333. Epub 2021 Jun 24.ABSTRACTThe aim of the present study was to detect copy number variations (CNVs) related to tumour progression and metastasis of urothelial carcinoma through whole-genome scanning. A total of 30 bladder cancer samples staged from pTa to pT4 were included in the study. DNA was extracted from freshly frozen tissue via standard phenol-chloroform extraction and CNV analysis was performed on two alternative platforms (CytoChip Oligo aCGH, 4x44K and Infinium OncoArray-500K BeadChip; Illumina, Inc.). Data were analysed with BlueFuse Multi software and...
Source: Experimental and Therapeutic Medicine - July 14, 2021 Category: General Medicine Authors: Victoria Spasova Boris Mladenov Simeon Rangelov Zora Hammoudeh Desislava Nesheva Dimitar Serbezov Rada Staneva Savina Hadjidekova Mihail Ganev Lubomir Balabanski Radoslava Vazharova Chavdar Slavov Draga Toncheva Olga Antonova Source Type: research