"Exp Ther Med"[ta]; +102 new citations
102 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: "Exp Ther Med"[ta] These pubmed results were generated on 2019/04/17PubMed comprises more than millions of citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites. (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - April 17, 2019 Category: General Medicine Tags: Report Source Type: research

Aberrant expression and high-frequency mutations of SHARPIN in nonmelanoma skin cancer.
Authors: Zheng Y, Yang Y, Wang J, Liang Y Abstract Squamous cell carcinoma (SCC) and basal cell carcinoma (BCC) have exhibited a marked increase in incidence in previous decades and are the most common malignancies in Caucasian populations. Src homology 3 and multiple ankyrin repeat domains protein-associated RH domain-interacting protein (SHARPIN) has been identified as a commonly overexpressed proto-oncogene in several types of visceral cancer. However, to the best of our knowledge, the functions of SHARPIN in nonmelanoma skin cancer (NMSC) have not been described. The present study aimed to investigate the expre...
Source: Experimental and Therapeutic Medicine - April 4, 2019 Category: General Medicine Tags: Exp Ther Med Source Type: research

A novel technique for the surgical management of intraocular lens-capsular bag-capsular tension ring complex dislocation.
Authors: Guo L, Wang C, Yang X Abstract The present study describes a surgical technique for the management of complex dislocation and subluxation of the intraocular lens (IOL)-capsular bag (CB)-capsular tension ring (CTR) complex. During the procedure, one double-curved suture was inserted through the sclera into the posterior chamber, passing through the anterior capsules and then into the anterior chamber. The suture lines were pulled out from above and below the IOL-CB-CTR complex and a knot was tied 1 mm away from the corneoscleral incision. The IOL-CB-CTR complex was repositioned by adjusting the suture lengt...
Source: Experimental and Therapeutic Medicine - April 4, 2019 Category: General Medicine Tags: Exp Ther Med Source Type: research

An Okinawan-based Nordic diet improves glucose and lipid metabolism in health and type 2 diabetes, in alignment with changes in the endocrine profile, whereas zonulin levels are elevated.
Authors: Ohlsson B Abstract The Okinawan-based Nordic diet has been developed to improve glucose metabolism. The aim of the present study was to summarize all anthropometric, subjective, and biochemical findings obtained following two different studies investigating this diet. The diet was administered i) as a single breakfast to healthy volunteers and ii) as a 12-week dietary intervention to patients with type 2 diabetes. The degree of satiety, sweet cravings, gastrointestinal symptoms, and health-related quality of life were estimated. Weight and blood pressures of participants were measured, and analyses includi...
Source: Experimental and Therapeutic Medicine - April 4, 2019 Category: General Medicine Tags: Exp Ther Med Source Type: research

Abnormal sub-pathways competitively regulated by lncRNAs contribute to postmenopausal osteoporosis.
Authors: Guan BG, Cai XX Abstract Abnormal sub-pathways competitively regulated by long non-coding RNAs (lncRNAs) for postmenopausal osteoporosis (PO) based on integration of lncRNA-mRNA expression data and pathway network topologies were investigated. Interesting lncRNA-mRNA pairs were selected by Pearsons correlation coefficient (PCC) algorithm on the basis of lncRNA-miRNA and miRNA-mRNA interactions and gene expression profiles. Then, lncRNAs in interesting pairs were embedded into pathway graphs as signature nodes by linking to their regulated-mRNAs, and lncRNA competitively regulated pathways (LCRPs) were gain...
Source: Experimental and Therapeutic Medicine - April 4, 2019 Category: General Medicine Tags: Exp Ther Med Source Type: research

Development of a poly-L-lactide salivary duct stent.
Authors: Ding YF, Tseng H, Su CH, Tai CC, Wu YY, Haung CJ, Lee CS, Hung SH Abstract Obstructive salivary gland diseases are common conditions that arise following the disruption of the secretary ductal system and usually results in the swelling and pain of the affected gland(s). There has been an increase in the use of sialendoscopy for the treatment and diagnosis of obstructive salivary gland infection. If damage occurs to a duct or papilla following sialendoscopy, a stent may be necessary to prevent restenosis and for maintaining the salivary duct open after complete sialendoscopy. Currently, there are only non-b...
Source: Experimental and Therapeutic Medicine - April 4, 2019 Category: General Medicine Tags: Exp Ther Med Source Type: research

Preservation of alveolar ridge after tooth extraction with hypoxia-inducible factor-1 α protein in a dog model.
Preservation of alveolar ridge after tooth extraction with hypoxia-inducible factor-1α protein in a dog model. Exp Ther Med. 2019 Apr;17(4):2913-2920 Authors: Tan L, Zhang Y, Huang Y, Luo Y, Liu Y Abstract Hypoxia-inducible factor (HIF)-1α protein, which is upregulated by hypoxia, serves an important role in angiogenesis during osteogenesis. The aim of the present study was to investigate the effect of HIF-1α on alveolar ridge preservation in a dog tooth extraction model. Six beagle dogs were used in the present study. The second and fourth premolar teeth of the lower jaws on both sid...
Source: Experimental and Therapeutic Medicine - April 4, 2019 Category: General Medicine Tags: Exp Ther Med Source Type: research

Successful application of next-generation sequencing for pre-natal diagnosis in a pedigree with chronic granulomatosis disease.
Authors: Peng F, Zhong L, Zhang B, Zou R, Nie S, Tian X, Deng S, He X Abstract The present study describes the successful application of next-generation sequencing (NGS) in the clinical diagnosis, pathogenic gene identification, treatment and pre-natal diagnosis in a pedigree with chronic granulomatosis disease (CGD). A 36-day-old infant, born to non-consanguineous Chinese parents, was admitted to hospital due to a neck lump for 10 days. A blood sample was collected for NGS to identify the molecular etiology. Sanger sequencing was performed for the patient and his relatives, including the parents. Amniotic fluid ex...
Source: Experimental and Therapeutic Medicine - April 4, 2019 Category: General Medicine Tags: Exp Ther Med Source Type: research

TIPE2 suppresses atherosclerosis by exerting a protective effect on macrophages via the inhibition of the Akt signaling pathway.
Authors: Li D, Tan Y Abstract Macrophage apoptosis and inflammation serve pivotal roles in the occurrence of atherosclerosis. However, the detailed underlying mechanism of macrophage action during atherosclerosis is poorly understood. Tumor necrosis factor-α-induced protein 8-like 2 (TIPE2) is a well-known negative regulator of the immune response. The current study assessed the association between TIPE2 and apoptosis-associated molecules in macrophages during atherosclerosis, as well as the role of TIPE2 in macrophage inflammation. RAW264.7 macrophages were subsequently transfected with a TIPE2 expression pl...
Source: Experimental and Therapeutic Medicine - April 4, 2019 Category: General Medicine Tags: Exp Ther Med Source Type: research

miR-204-5p promotes diabetic retinopathy development via downregulation of microtubule-associated protein 1 light chain 3.
Authors: Mao XB, Cheng YH, Xu YY Abstract Diabetic retinopathy (DR) is a chronic and progressive complication of diabetes mellitus. DR impairs sight due to neuronal and vascular dysfunction in the retina. It is critical to investigate the pathogenesis of DR to develop effective treatment. In the present study, a streptozotocin (STZ)-induced diabetic rat model was constructed and the expression of microRNA (miR)-204-5p and vascular endothelial growth factor (VEGF) were determined. Immunohistochemistry, reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blotting were employed to detect...
Source: Experimental and Therapeutic Medicine - April 4, 2019 Category: General Medicine Tags: Exp Ther Med Source Type: research

Proteasome inhibitor MG132 inhibits the process of renal interstitial fibrosis.
In conclusion, MG132 reduced mRNA and protein expression of fibrosis-associated factors. It can successfully inhibit the inflammatory reaction induced by TGF-β via the Smad signaling pathway. These results indicate that MG132 appears to have a potent effect in counteracting renal fibrosis. MG132 may be applied in the treatment of patients with chronic kidney disease. PMID: 30936965 [PubMed] (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - April 4, 2019 Category: General Medicine Tags: Exp Ther Med Source Type: research

Changes of Th17/Treg ratio in the transition of chronic hepatitis B to liver cirrhosis and correlations with liver function and inflammation.
Authors: Mou H, Wu S, Zhao G, Wang J Abstract The changes in ratio of T helper 17 cells (Th17) to Treg cells in the transition of chronic hepatitis B (CHB) to liver cirrhosis, and the correlations with liver function and inflammation were investigated. A total of 20 normal subjects (control group), 35 CHB patients (CHB group) and 40 post-hepatitis liver cirrhosis patients (liver cirrhosis group) were enrolled into this study. Liver function was measured through the levels of alanine aminotransferase (ALT) and aspartic transaminase (AST), and the hematoxylin and eosin (H&E) staining method was used to detect the...
Source: Experimental and Therapeutic Medicine - April 4, 2019 Category: General Medicine Tags: Exp Ther Med Source Type: research

Downregulation of nucleolar and spindle-associated protein 1 expression suppresses liver cancer cell function.
Authors: Wang Y, Ju L, Xiao F, Liu H, Luo X, Chen L, Lu Z, Bian Z Abstract The aim of the present study was to determine the role of nucleolar and spindle-associated protein 1 (NuSAP1) in human liver cancer. NuSAP1 expression was determined by reverse transcription-quantitative polymerase chain reaction (RT-qPCR), western blotting and immunohistochemistry in hepatocellular carcinoma (HCC) and adjacent tissues. The expression of NuSAP1 gene was detected by RT-qPCR in liver cancer cell lines. Expression information for NuSAP1 was determined using the UALCAN and Oncomine databases. The Kaplan-Meier plotter and The Can...
Source: Experimental and Therapeutic Medicine - April 4, 2019 Category: General Medicine Tags: Exp Ther Med Source Type: research

Effects of intrahepatic cholestasis of pregnancy on hepatic function, changes of inflammatory cytokines and fetal outcomes.
Authors: Wang L, Lu Z, Zhou X, Ding Y, Guan L Abstract The effects of intrahepatic cholestasis of pregnancy (ICP) on hepatic function, changes of inflammatory cytokines and fetal outcomes were studied. In total, 663 pregnant women admitted to Daqing Longnan Hospital from July 2016 to December 2017 were selected. There were, 40 cases with ICP enrolled in the observation group, and 40 normal pregnant women were recruited in the normal group. They were also grouped according to hepatic function and inflammatory cytokines, with 40 cases in each group. Neonatal Apgar scores were recorded. The correlations of serum choly...
Source: Experimental and Therapeutic Medicine - April 4, 2019 Category: General Medicine Tags: Exp Ther Med Source Type: research

A prospective study on peptide mapping of human fatigue saliva markers based on magnetic beads.
Authors: Xu Y, Xiao D, Zhang H, He L, Gu Y, Peng X, Gao X, Liu Z, Zhang J Abstract In order to explore convenient and stable fatigue markers, we studied various high-molecular-weight peptide fragments under fatigue state and non-fatigue state in the saliva using time of flight mass spectrometry. The saliva samples were collected from 10 healthy volunteers that were in the condition of fatigue and non-fatigue, respectively. Moreover, the time of flight mass spectrometry was conducted using two kinds of sample treatment methods, the magnetic beads enrichment (MB) and direct detection of stock solution. This was follo...
Source: Experimental and Therapeutic Medicine - April 4, 2019 Category: General Medicine Tags: Exp Ther Med Source Type: research

Peptide P7 inhibits the bFGF-stimulated proliferation and invasion of SKOV3 cells.
In conclusion, the present results suggested that P7, which, at least in part, acts through inhibition of bFGF, may have a potential therapeutic application in epithelial ovarian cancer. PMID: 30936970 [PubMed] (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - April 4, 2019 Category: General Medicine Tags: Exp Ther Med Source Type: research

Effects of berberine on glucose-lipid metabolism, inflammatory factors and insulin resistance in patients with metabolic syndrome.
Authors: Cao C, Su M Abstract Effects of berberine on glucose-lipid metabolism, inflammatory factors and insulin resistance in patients with metabolic syndrome were investigated. Eighty patients with metabolic syndrome treated in Linyi Central Hospital from January 2017 to December 2017 were selected and divided into control group (n=40) and observation group (n=40). Patients in control group were treated with regular therapy using the Western medicine and drugs, while those in observation group, based on the treatment in control group, were treated with berberine. Changes in relevant indexes to blood glucose and l...
Source: Experimental and Therapeutic Medicine - April 4, 2019 Category: General Medicine Tags: Exp Ther Med Source Type: research

Cyclosporin population pharmacokinetics in pediatric refractory nephrotic syndrome based on real-world studies: Effects of body weight and spirolactone administration.
In conclusion, in the present study, a cyclosporin PPK model for patients with PRNS was successfully constructed, and the presence of a clinically significant interaction between spirolactone and cyclosporin in PRNS patients was determined based on real-world studies, indicating that concomitant medication with spirolactone was able to reduce cyclosporin clearance in the patients with PRNS. PMID: 30936972 [PubMed] (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - April 4, 2019 Category: General Medicine Tags: Exp Ther Med Source Type: research

High throughput analysis to identify key gene molecules that inhibit adipogenic differentiation and promote osteogenic differentiation of human mesenchymal stem cells.
Authors: Peng WX, Gao CH, Huang GB Abstract The present study investigated the key genes, which cause switch from adipogenic to osteogenic differentiation of human mesenchymal stem cells (hMSCs). The transcriptomic profile of hMSCs samples were collected from Array Express database. Differential expression network was constructed by calculating the Pearson's correlation coefficient and ranked according to their topological features. The top 5% genes with degree ≥2 were selected as ego genes. Following the KEGG pathway enrichment analysis and the relevant miRNAs prediction, the miRNA-mRNA-pathway networks were co...
Source: Experimental and Therapeutic Medicine - April 4, 2019 Category: General Medicine Tags: Exp Ther Med Source Type: research

Constructing differential co-expression network to predict key pathways for myocardial infarction.
In conclusion, the optimal pathways (MAPK signaling pathway, cytokine-cytokine receptor interaction, and calcium signaling pathway) might play important roles in the progression of UA/MI. PMID: 30936974 [PubMed] (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - April 4, 2019 Category: General Medicine Tags: Exp Ther Med Source Type: research

Identification of lncRNA competitively regulated subpathways in myocardial infarction.
Authors: Wu X, Sun L, Wang Z Abstract The functions of long non-coding RNAs (lncRNAs) in myocardial infarction (MI) remain largely unknown. Thus, we used the subp athway-LINCE method to characterize the potential roles of lncRNAs in MI. Candidate lncRNA-mRNA interactions were obtained from miRNA-mRNA interactions and lncRNA-miRNA interactions. Then the lncRNA and mRNA co-expression relationship pairs (LncGenePairs) were screened from the lncRNAs and mRNA intersections, which were extracted through candidate lncRNA-mRNA interactions and sample gene expression profiles. The lncRNAs in LncGenePairs were embedded into ...
Source: Experimental and Therapeutic Medicine - April 4, 2019 Category: General Medicine Tags: Exp Ther Med Source Type: research

Analysis of thrombelastogram-guided medication in patients with coronary heart disease after percutaneous coronary intervention.
Authors: Li Y, Chang H, Ni L, Xue P, Li C, Yuan L, Cui H, Yu C Abstract Effects of thrombelastogram-guided (TEG-guided) clopidogrel and aspirin on major adverse cardiovascular events (MACE) after percutaneous coronary intervention (PCI) were investigated. A retrospective analysis was performed on 203 patients undergoing PCI interventional therapy in the Cardiovascular Medicine of Weihai Central Hospital from February 2015 to September 2016. The patients were treated with clopidogrel and aspirin for anti-thrombus therapy. Among them, 104 patients who had TEG detection of anticoagulant effects for guiding medication ...
Source: Experimental and Therapeutic Medicine - April 4, 2019 Category: General Medicine Tags: Exp Ther Med Source Type: research

Effect of prealbumin level on mortality in heatstroke patients.
This study investigated whether serum prealbumin level is associated with mortality in heatstroke patients. A retrospective study of 102 heatstroke patients admitted to the ICU in the Affiliated Changzhou No. 2 People's Hospital of Nanjing Medical University from June 2010 to November 2017 was performed they were divided into normal serum prealbumin group (n=79) and low prealbumin group (n=23) according to the difference in PA expression. The clinical data, laboratory inspection data, invasive positive pressure ventilation (IPPV), co-infection, shock and length of ICU stay during the ICU were compared between the two group...
Source: Experimental and Therapeutic Medicine - April 4, 2019 Category: General Medicine Tags: Exp Ther Med Source Type: research

Therapeutic effect of resveratrol on mice with depression.
Authors: Gu Z, Chu L, Han Y Abstract The effect and mechanism of resveratrol on depression-like behavior in mice with depression were investigated. A mouse model of depression was established by social isolation combined with chronic unpredictable stress. The mice were randomly divided into the control group, the model group, the low dose group, the medium dose group and the high dose group. The rats in the low, medium and high dose groups were intraperitoneally injected with resveratrol 10, 20 and 30 mg/kg, respectively. The control and model groups were intraperitoneally injected with an equal volume of normal sa...
Source: Experimental and Therapeutic Medicine - April 4, 2019 Category: General Medicine Tags: Exp Ther Med Source Type: research

Expression and clinical significance of 5-HT and 5-HT3R in the intestinal mucosa of patient with diarrhea-type irritable bowel syndrome.
Authors: Fu R, Chen M, Chen Y, Mao G, Liu S Abstract Expression levels and clinical significance of 5-HT and 5-HT3R in the intestinal mucosa tissue of patients with diarrhea-type irritable bowel syndrome (D-IBS) were investigated. A retrospective analysis was performed on 46 tissue specimens (observation group) of the intestinal mucosa of patients with D-IBS, who were diagnosed in the Tongde Hospital of Zhejiang Province and received colonoscopy from March 2016 to December 2017, and 18 tissue specimens (control group) of the intestinal mucosa of healthy subjects who received physical examinations. The expression le...
Source: Experimental and Therapeutic Medicine - April 4, 2019 Category: General Medicine Tags: Exp Ther Med Source Type: research

Association between gallstone and cardio-cerebrovascular disease: Systematic review and meta-analysis.
In conclusion, the presence of GD was associated with an increased risk of CVD incidence and prevalence. The association may be influenced by age and sex. These findings suggest that individuals identified with cardio-cerebrovascular disease should be evaluated for GD. PMID: 30936980 [PubMed] (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - April 4, 2019 Category: General Medicine Tags: Exp Ther Med Source Type: research

Loss of interleukin-6 enhances the inflammatory response associated with hyperoxia-induced lung injury in neonatal mice.
Authors: Li H, Wang G, Lin S, Wang C, Zha J Abstract In bronchopulmonary dysplasia (BPD), decreased angiogenesis and alveolarization is associated with pulmonary cell death and inflammation. It is commonly observed in premature infants who required mechanical ventilation and oxygen therapy. Since enhanced interleukin-6 (IL-6) expression has been reported in infants with BPD, it was hypothesized that a decrease in IL-6 may enhance lung inflammation and decrease hyperoxia-induced neonatal lung injury in mice. In the current study, newborn wild-type (WT) and IL-6 null mice were treated with 85% O2 (hyperoxia) or 21% O...
Source: Experimental and Therapeutic Medicine - April 4, 2019 Category: General Medicine Tags: Exp Ther Med Source Type: research

miR-29a/b cluster suppresses high glucose-induced endothelial-mesenchymal transition in human retinal microvascular endothelial cells by targeting Notch2.
Authors: Zhang J, Zeng Y, Chen J, Cai D, Chen C, Zhang S, Chen Z Abstract Several studies have previously reported that endothelial cells contributed to pathological fibrosis in proliferative diabetic retinopathy (PDR) through endothelial-mesenchymal transition (EndMT); however, the precise mechanism of this interaction has not been completely elucidated. The present study investigated the expression of microRNA (miR)-29a/b cluster in human retinal microvascular endothelial cells (HRMECs) and examined its functional role in high glucose (HG)-induced EndMT. HRMECs were exposed to glucose at concentrations of 5, 15, ...
Source: Experimental and Therapeutic Medicine - April 4, 2019 Category: General Medicine Tags: Exp Ther Med Source Type: research

NL1 expression level in Nrx1 β and the excitability of PV interneurons in mice with POCD.
NL1 expression level in Nrx1β and the excitability of PV interneurons in mice with POCD. Exp Ther Med. 2019 Apr;17(4):3117-3123 Authors: Tian M, Zhou Y, Qiu L, Liu F, Jin L, Li W, Zhang L Abstract Effects of NL1 on the expression level of neurexin-1β (Nrx1β) and the excitability of parvalbumin (PV) interneurons in mice with postoperative cognitive dysfunction (POCD) were explored. Eighty 10-month-old C57BL/6 male mice were randomly divided into four groups: control group, control+empty vector group (Control+EV), anesthesia surgery+empty vector group (POCD+EV group) and anesthesia+NL1 ove...
Source: Experimental and Therapeutic Medicine - April 4, 2019 Category: General Medicine Tags: Exp Ther Med Source Type: research

Value of MRI enhanced FLAIR sequence examination combined with CSF TNF- α detection in the early diagnosis of neonatal purulent meningitis.
This study aimed to investigate the value of MRI enhanced fluid-attenuated inversion recovery (FLAIR) combined with TNF-α in cerebrospinal fluid in the diagnosis of neonatal purulent meningitis. Fifty neonates with purulent meningitis were randomly selected in the purulency group, 50 patients with viral meningitis (VM) in the virus group and 50 neonates without purulent meningitis in the no meningitis group. All neonates were selected from April 2015 to May 2018 in Women and Children's Hospital (Linyi, China). Clinical baseline data of the three groups were compared, and logistic regression analysis was conducted to ...
Source: Experimental and Therapeutic Medicine - April 4, 2019 Category: General Medicine Tags: Exp Ther Med Source Type: research

Correlation of miR-21 and BNP with pregnancy-induced hypertension complicated with heart failure and the diagnostic value.
Authors: Kan C, Cao J, Hou J, Jing X, Zhu Y, Zhang J, Guo Y, Chen X Abstract Correlation of miR-21 and B-type natriuretic peptide (BNP) with pregnancy-induced hypertension (PIH) complicated with heart failure and the diagnostic value was investigated. Sixty patients with PIH complicated with heart failure admitted to Affiliated Hospital of Chengde Medical University from July 2016 to July 2017 were enrolled as the experimental group, and 35 normal pregnant women as the control group. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) method was used to determine the expression level of plasma mi...
Source: Experimental and Therapeutic Medicine - April 4, 2019 Category: General Medicine Tags: Exp Ther Med Source Type: research

Pretreatment with neuregulin-1 improves cardiac electrophysiological properties in a rat model of myocardial infarction.
Authors: Rao P, Liu Z, Duan H, Dang S, Li H, Zhong L, Wang X, Wang L, Wang X Abstract Neuregulin-1 (NRG-1) is considered to be a potential therapeutic agent for cardiovascular diseases due to its diverse protective effects. The aim of the present study was to investigate the effect of NRG-1 on cardiac electrophysiology in rats with myocardial infarction (MI). The rats were randomly divided into three groups: The sham operation group (SO; n=8); MI group (n=8); and the MI with recombinant human NRG (rhNRG)-1 administration group (NRG-1 group; 10 µg/kg; n=8). A rat MI model was established via ligation of the le...
Source: Experimental and Therapeutic Medicine - April 4, 2019 Category: General Medicine Tags: Exp Ther Med Source Type: research

PDCD4 expression in coronary atherosclerosis rat models and its mechanism.
This study investigated the expression of programmed cell death protein 4 (PDCD4) in rat models of coronary atherosclerosis (AS) and analyzed its role and mechanism. A total of 80 Wistar rats were selected and divided into the control group (n=40) and research group (n=40) according to the principle of similar body weight, of which coronary AS models were established in rats in the research group. PDCD4 expression in coronary artery tissues was detected using western blotting, and the expression of interleukin-6 (IL-6) and IL-8 in the coronary artery tissues were measured by means of reverse transcription-polymerase chain ...
Source: Experimental and Therapeutic Medicine - April 4, 2019 Category: General Medicine Tags: Exp Ther Med Source Type: research

Prioritization of candidate metabolites for postmenopausal osteoporosis using multi-omics composite network.
Authors: Zhang C, Wang Y, Zhang CL, Wu HR Abstract Risk metabolites of postmenopausal osteoporosis (PO) were explored to offer a theoretical basis for future therapy. The data E-GEOD-7429 were downloaded from ArrayExpress database. In total 20 samples deprived from postmenopausal women having low or high bone mineral density (BMD) were covered in this expression profile. After screening of differentially expressed genes (DEGs), gene-gene network was constructed taking the intersection between the DEGs and genes in the seed protein-protein interaction network. Then, the other five networks were established, includin...
Source: Experimental and Therapeutic Medicine - April 4, 2019 Category: General Medicine Tags: Exp Ther Med Source Type: research

Sub-pathway based approach to systematically track candidate sub-pathway biomarkers for heart failure.
Authors: Han DM Abstract Identification of potential novel biomarkers for heart failure was undertaken using a sub-pathway based method. To realize this goal, heart failure-relevant dataset, reference pathways, and lncRNA-miRNA-mRNA interactions were firstly recruited. Secondly, the informative pathways were extracted relying on KEGG pathways and the mRNAs in the PCC-weighted lncRNA-mRNA interactions. Thirdly, lncRNA-regulated sub-pathways were dissected after construction of condition-specific lncRNA competitively regulated pathways (LCRP). To detect crucial heart failure-relevant lncRNAs, degree analysis was cond...
Source: Experimental and Therapeutic Medicine - April 4, 2019 Category: General Medicine Tags: Exp Ther Med Source Type: research

Dynamic changes and clinical significance of LXA4 in the perioperative period of cardiopulmonary bypass.
Authors: Wu M, Feng Z, Liao J, Zhou H, Wang J Abstract Dynamic changes in lipoxin A4 (LXA4) in child patients with congenital heart disease (CHD), in the perioperative period of cardiopulmonary bypass (CPB) were studied. Peripheral blood was collected from 16 child patients (CPB group) before operation (Tc), after operation (T0), at 1 day after operation (T1), at 3 days after operation (T3), and at 7 days after operation (T7); and from 17 children with no CHD (control group). The level of LXA4 in peripheral blood was detected via enzyme-linked immunosorbent assay (ELISA). Clinical data of the child patients were co...
Source: Experimental and Therapeutic Medicine - April 4, 2019 Category: General Medicine Tags: Exp Ther Med Source Type: research

Expression of inositol-requiring enzyme 1 β is downregulated in azoxymethane/dextran sulfate sodium-induced mouse colonic tumors.
In conclusion, the downregulation of IRE1β and MUC2 may reduce the ability of colon tissues to resist inflammation, thus promoting the occurrence and development of colonic tumors. PMID: 30936991 [PubMed] (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - April 4, 2019 Category: General Medicine Tags: Exp Ther Med Source Type: research

The risk factors of portal vein thrombosis in patients with liver cirrhosis.
This study was designed to identify and assess risk factors for portal vein thrombosis (PVT) in patients with cirrhosis. A total of 98 cirrhosis patients with PVT were identified and 101 cirrhosis patients without PVT were chosen as the control group in this retrospective study. Several variables were measured and the two groups PVT and non-PVT were compared statistically. PVT was identified in 98 patients (10%). Significant differences in hematocrit, international normalized ratio, albumin, bilirubin and glucose were determined between the groups (P0.05). PMID: 30936992 [PubMed] (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - April 4, 2019 Category: General Medicine Tags: Exp Ther Med Source Type: research

Tip60-siRNA regulates ABCE1 acetylation to suppress lung cancer growth via activation of the apoptotic signaling pathway.
Authors: Liang Z, Yu Q, Ji H, Tian D Abstract Lung cancer is a leading cause of cancer-associated mortality and morbidity worldwide. Previous studies have suggested that ATP-binding cassette transporter E1 (ABCE1) acetylation is upregulated in the tissues and cells of lung cancer and is associated with the prognosis of patients with lung cancer. The aim of the present study was to investigate the association between Tat interactive protein 60 kDa (Tip60) expression and ABCE1 acetylation, and the effect of Tip60 on the biological functions of A549 lung carcinoma cells. The expression levels of Tip60 and ABCE1 acetyl...
Source: Experimental and Therapeutic Medicine - April 4, 2019 Category: General Medicine Tags: Exp Ther Med Source Type: research

LncRNA PRINS is involved in the development of nephropathy in patients with diabetes via interaction with Smad7.
Authors: Jiao H, Xie D, Qiao Y Abstract Long non-coding RNA psoriasis-susceptibilityrelated RNA gene induced by stress (PRINS) is known to be involved in kidney ischemia reperfusion injury. The aim of the current study was to investigate the potential role of PRINS in diabetic nephropathy. The relative mRNA expression level of PRINS and SMAD family member 7 (Smad7) was examined in patients with diabetes, including patients without obvious complications (n=43), patients with diabetic nephropathy (n=33), diabetic retinopathy (n=37), diabetic cardiomyopathy (n=29), diabetic lung disease (n=38) and healthy controls (n=...
Source: Experimental and Therapeutic Medicine - April 4, 2019 Category: General Medicine Tags: Exp Ther Med Source Type: research

Analysis of promoter methylation and epigenetic regulation of miR-32 in colorectal cancer cells.
Authors: Wu W, Ye S, Tan W, Zhou Y, Quan J Abstract MicroRNA-32 (miR-32) is upregulated in colorectal cancer (CRC) tissues; its overexpression leads to increased cell proliferation, migration and invasion, as well as reduced apoptosis of CRC cells, at least partly by inhibiting the target gene phosphatase and tensin homolog. However, the mechanisms of its upregulation have remained elusive. In the present study, the effects of methylation and acetylation on the expression of miR-32 were investigated. The promoter methylation status of miR-32 in the CRC cell lines HT-29 and HCT-116 and the normal colonic epithelial ...
Source: Experimental and Therapeutic Medicine - April 4, 2019 Category: General Medicine Tags: Exp Ther Med Source Type: research

Resveratrol downregulates the TLR4 signaling pathway to reduce brain damage in a rat model of focal cerebral ischemia.
In conclusion, the present data suggest that resveratrol reduces inflammation, BBB disruption and brain damage in rats following focal cerebral ischemia. Additionally, the neuroprotective effects of resveratrol against cerebral ischemia may be associated with downregulation of the TLR4 pathway. PMID: 30936996 [PubMed] (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - April 4, 2019 Category: General Medicine Tags: Exp Ther Med Source Type: research

Efficiency evaluation and safety monitoring of tailored rapid potassium supplementation strategy for fatal severe hypokalemia.
In conclusion, these results demonstrate that the tailored rapid potassium supplementation strategy shortened the time of hypokalemia and is a safe and better treatment option to remedy life-threatening arrhythmia caused by severe hypokalemia with a high success rate. PMID: 30936997 [PubMed] (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - April 4, 2019 Category: General Medicine Tags: Exp Ther Med Source Type: research

Decreased expression of microRNA-30b promotes the development of pulpitis by upregulating the expression of interleukin-6 receptor.
Authors: Zhang N, Zhang Q, Yang W, Miao L, Wang N, Wei S, Ge J, Li X, Wu J Abstract The present study aimed to examine the expression of interleukin-6 receptor (IL-6R) mRNA and protein in pulp tissues, blood and saliva from patients with pulpitis. It also investigated the association between IL-6R and microRNA (miR)-30b, as well as their effects on pulpitis. A total of 28 patients with pulpitis were recruited into the experimental group and 16 subjects with no pulpitis who also underwent tooth extraction were recruited into the control group. Pulp tissues, plasma and saliva were collected from all participants. Rev...
Source: Experimental and Therapeutic Medicine - April 4, 2019 Category: General Medicine Tags: Exp Ther Med Source Type: research

miRNA-155 expression and role in pathogenesis in spinal tuberculosis-induced intervertebral disc destruction.
Authors: Yang C, Shi Z, Hu J, Wei R, Yue G, Zhou D Abstract The current study aimed to investigate microRNA-155 (miR-155) expression in spinal tuberculosis-induced intervertebral disc destruction and its regulatory role in disease pathogenesis. A total of 26 patients with intervertebral disc destruction induced by spinal tuberculosis and 31 healthy individuals were included. Reverse transcription-quantitative polymerase chain reactions, western blot analysis and ELISA were performed to detect mRNA and protein expression levels. A bioinformatics analysis was applied to predict the upstream regulator of matrix metall...
Source: Experimental and Therapeutic Medicine - April 4, 2019 Category: General Medicine Tags: Exp Ther Med Source Type: research

Association of toll-like receptor 9 expression with prognosis of systemic lupus erythematosus.
Authors: Yuan Y, Zhao L, Ye Z, Ma H, Wang X, Jiang Z Abstract The current study assessed the association between toll-like receptor 9 (TLR9) and systemic lupus erythematosus (SLE) and subsequently determined the predictive value of TLR9 in assessing the prognosis of SLE. A total of 90 newly diagnosed patients with SLE and 49 healthy control subjects were enrolled in the current study. The expression of TLR9 mRNA was measured in whole blood samples from patients and controls. All patients were followed up for ≥2 years and their clinical parameters were recorded. After 2 years, 30 patients were randomly chosen fro...
Source: Experimental and Therapeutic Medicine - April 4, 2019 Category: General Medicine Tags: Exp Ther Med Source Type: research

Carnosine improves diabetic retinopathy via the MAPK/ERK pathway.
Authors: Guo Y, Guo C, Ha W, Ding Z Abstract Diabetic retinopathy (DR) is one of the most common causes of blindness in developed countries. Due to its asymptomatic onset and progressive disease course, DR is typically diagnosed at a late stage when treatment options are limited and therefore often results in irreversible blindness. Studies have demonstrated that carnosine may prevent and treat DR. In a previous study, the positive effect of carnosine on DR was determined and it was revealed that there may be an association between carnosine and the mitogen-activated protein kinase (MAPK)/extracellular signal relat...
Source: Experimental and Therapeutic Medicine - April 3, 2019 Category: General Medicine Tags: Exp Ther Med Source Type: research

Prognostic value of the mRNA expression of members of the HSP90 family in non-small cell lung cancer.
Authors: Liu K, Kang M, Li J, Qin W, Wang R Abstract The aim of the present study was to investigate the potential prognostic value of members of the heat shock protein (HSP)90 family in non-small cell lung cancer (NSCLC) patients. The mRNA expression profiles of 1,926 NSCLC patients, which was available from the Kaplan-Meier plotter database, were included in the study. High expression of HSP90AA1 mRNA was significantly associated with a poorer rate of overall survival (OS) for all NSCLC patients [hazard ratio (HR), 1.21; 95% confidence interval (CI): 1.06-1.37; P=0.004], as well as for patients with adenocarcinom...
Source: Experimental and Therapeutic Medicine - April 3, 2019 Category: General Medicine Tags: Exp Ther Med Source Type: research

Protective effect of ethyl vanillin against A β-induced neurotoxicity in PC12 cells via the reduction of oxidative stress and apoptosis.
Protective effect of ethyl vanillin against Aβ-induced neurotoxicity in PC12 cells via the reduction of oxidative stress and apoptosis. Exp Ther Med. 2019 Apr;17(4):2666-2674 Authors: Zhong L, Tong Y, Chuan J, Bai L, Shi J, Zhu Y Abstract Increased aggregation of β-amyloid (Aβ) peptides induces oxidative stress, which is considered a major contributor in the development of Alzheimer's disease (AD). Prevention of Aβ-induced neurotoxicity is proposed as a possible modality for treatment of AD. The present study aimed to elucidate possible effects of ethyl vanillin (EVA), an analog of ...
Source: Experimental and Therapeutic Medicine - April 3, 2019 Category: General Medicine Tags: Exp Ther Med Source Type: research

Utility of brain CT for predicting delayed encephalopathy after acute carbon monoxide poisoning.
This study identified computed tomography (CT) findings in the prediction of DEACMP development. Adults with CO poisoning admitted to Beijing Chaoyang Hospital, Shenyang Ninth People's Hospital, Shanxi Second People's Hospital and Shandong Provincial Hospital in China from January 2013 to January 2016 were retrospectively analyzed. Baseline demographic and clinical characteristics were extracted from the medical records. Brain CT imaging was carried out within 24 h of admission, and an integrated CT score was calculated to semi-quantify lesion severity. Patients were followed-up for 6 months. Baseline characteristics and C...
Source: Experimental and Therapeutic Medicine - April 3, 2019 Category: General Medicine Tags: Exp Ther Med Source Type: research