IL-17A and GDF15 are able to induce epithelial-mesenchymal transition of lung epithelial cells in response to cigarette smoke.
Authors: Jiang G, Liu CT, Zhang WD Abstract Smoking is one of the primary causes of chronic obstructive pulmonary disease (COPD). Sustained active epithelial-mesenchymal transition (EMT) in COPD may explain the core pathophysiology of airway fibrosis and why lung cancer is so common among smokers. Interleukin (IL)-17A and growth/differentiation factor (GDF)15 have been reported to be biomarkers of COPD; however, the role of IL-17A and GDF15 in EMT remains unclear. The aim of the present study was to investigate the role of IL-17A and GDF15 in the pathogenesis of COPD. It was demonstrated that IL-17A and GDF15 are u...
Source: Experimental and Therapeutic Medicine - July 8, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Protective effects of nicorandil against cerebral injury in a swine cardiac arrest model.
Authors: Zhu F, Zhong X, Zhou Y, Hou Z, Hu H, Liang L, Chen J, Chen Q, Ji X, Shang D Abstract The present study investigated the effects of nicorandil on cerebral injury following cardiopulmonary resuscitation (CPR) in a swine model of cardiac arrest. CPR was performed on swine following 4 min induced ventricular fibrillation. Surviving animals were randomly divided into 3 groups: A nicorandil group (n=8), a control group (n=8) and a sham group (n=4). The sham group underwent the same surgical procedure to imitate cardiac arrest, but ventricular fibrillation was not induced. When the earliest observable return of s...
Source: Experimental and Therapeutic Medicine - July 8, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Smoking and drinking influence the advancing of ischemic stroke disease by targeting PTGS2 and TNFAIP3.
Authors: Miao Z, Guo M, Zhou S, Sun X, Wang F, Lu H, Cui Z Abstract In the present study, we explored the influence of cigarette smoking and alcohol drinking on gene expression level and related functions and pathways on the development of ischemic stroke (IS) disease. The gene expression profile of E-GEOD-22255 was obtained from 20 IS samples (7 patients without smoking or drinking history and 13 patients with smoking or drinking history) and 20 controls (9 normal controls without smoking or drinking history and 11 controls with smoking or drinking history). The correlation degree between gene expression and group...
Source: Experimental and Therapeutic Medicine - July 8, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Propofol improves the function of natural killer cells from the peripheral blood of patients with esophageal squamous cell carcinoma.
Authors: Zhou M, Dai J, Zhou Y, Wu J, Xu T, Zhou D, Wang X Abstract Postoperative immunosuppression is associated with the recurrence and metastasis of esophageal squamous cell carcinoma (ESCC). Propofol is a commonly used intravenous anesthetic and has been reported to be associated with immunosuppression; however, little is known about its effect on innate immune cells during the postoperative period in patients with ESCC. The aim of the present study was to investigate the effects of propofol on the phenotype and cytotoxicity of natural killer (NK) cells derived from the peripheral blood of patients with ESCC. T...
Source: Experimental and Therapeutic Medicine - July 8, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Establishment of a novel rat model of blast-related diffuse axonal injury.
Authors: Zhang JH, Gu JW, Li BC, Gao FB, Liao XM, Cui SJ Abstract Although studies concerning blast-related traumatic brain injury (bTBI) have demonstrated the significance of diffuse axonal injury (DAI), no standard models for this type of injury have been widely accepted. The present study investigated a mechanism of inducing DAI through real blast injury, which was achieved by performing instantaneous high-speed swinging of the rat head, thus establishing a stable animal model of blast DAI. Adult Sprague-Dawley rats weighing 150±10 g were randomly divided into experimental (n=16), control (n=10) and sham ...
Source: Experimental and Therapeutic Medicine - July 8, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

A cynomolgus monkey model of carotid atherosclerosis induced by puncturing and scratching of the carotid artery combined with a high-fat diet.
Authors: Zhang L, Zeng Y, Qi J, Xu Y, Zhang S, Zhou X, Ping R, Fu S Abstract Cardio-cerebrovascular disease is one of the three major causes of mortality in humans and constitutes a major socioeconomic burden. Carotid atherosclerosis (CAS) is a very common lesion of the arterial walls, which leads to narrowing of the arteries, in some cases occluding them entirely, increasing the risk of cardiovascular events. The aim of the present study was to evaluate a cynomolgus monkey model of carotid atherosclerosis (CAS) induced by puncturing and scratching combined with a high-fat diet. A total of 12 cynomolgus monkeys wer...
Source: Experimental and Therapeutic Medicine - July 8, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Early growth response factor-1 DNA enzyme 1 inhibits the formation of abdominal aortic aneurysm in rats.
Authors: Wang S, Dong H, Liu C, Xu G, Hu X, Fan Y, Chen L Abstract The aim of the present study was to characterize the effects of early growth response factor-1 DNA enzyme (EDRz) in a rat abdominal aortic aneurysm (AAA) model to determine the mechanism by which EDRz inhibits AAA and affects the formation of AAA by regulating the activity of matrix metalloproteinase (MMP)-2 and MMP-9. EDRz was transfected into the abdominal aorta of rats using the jetPRIME transfection reagent following infusion with elastase. Fluorescent microscopy, hematoxylin and eosin staining, ultrastructural analysis, reverse transcription-qu...
Source: Experimental and Therapeutic Medicine - July 8, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Identification of differential modules in ankylosing spondylitis using systemic module inference and the attract method.
In conclusion, the present study successfully identified one differential module for AS that may be a potential marker for AS target therapy and provide insights for future research on this disease. PMID: 29977361 [PubMed] (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - July 8, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Mechanism of melatonin combined with calcium carbonate on improving osteoporosis in aged rats.
Authors: Zhu F, Liu Z, Ren Y Abstract The effects of melatonin and calcium carbonate on aged rats with osteoporosis (OP) were assessed. Forty female Sprague-Dawley (SD) rats aged 15 months were randomly divided into a model group (group OP), melatonin group (group M), calcium carbonate group (group Ca) and melatonin combined with calcium carbonate group (group M+Ca), while 10 rats aged 3 months were set as the control group (group NC). The changes of bone density and bone mineral level of lumbar vertebra and bilateral femur in rats of each group were observed. The levels of serum calcium, phosphorus, superoxide dis...
Source: Experimental and Therapeutic Medicine - July 8, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Glycerol fructose combined with vitamin B6 is beneficial to postoperative recovery of patients with cerebral aneurysm.
Authors: Chen X, Lei T Abstract The aim of the present study is to investigate whether glycerol fructose combined with vitamin B6 is beneficial to the postoperative recovery of patients with cerebral aneurysm (CA). A total of 134 patients receiving embolization of CA in the Central Hospital of Wuhan between February, 2013 and June, 2015 and were divided into observation and control groups according to the random number table method, with 67 cases in each group. The control was given vitamin B6 routine treatment, while the observation group received glycerol fructose on the basis of treatment in the control group. T...
Source: Experimental and Therapeutic Medicine - July 8, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Serum STLT-1 and bilirubin levels in patients with acute coronary syndrome and correlation with prognosis.
Authors: Fu R, Song X, Su D, Li S, Gao L, Ji C Abstract The serum triggering receptor expressed on myeloid cell (TREM)-like transcription factor-1 [soluble TREM-like transcript-1 (STLT-1)] and bilirubin levels were investigated in patients with acute coronary syndrome and the correlation with prognosis. A total of 125 patients of acute coronary syndrome admitted to the Department of Cardiology in People's Hospital of Rizhao were selected, including 45 cases with ST-segment elevation myocardial infarction (STEMI), 36 cases with non-ST-elevation myocardial infarction (NSTEMI) and 44 cases with unstable angina pectori...
Source: Experimental and Therapeutic Medicine - July 8, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Knockdown of RWD domain containing 3 inhibits the malignant phenotypes of glioblastoma cells via inhibition of phosphoinositide 3-kinase/protein kinase B signaling.
In conclusion, the current study demonstrated for the first time that inhibition of RWDD3 expression inhibited glioblastoma progression, at least partly, via suppressing the PI3K/AKT signaling activity, and thus RWDD3 may be a novel potential therapeutic target for glioblastoma. PMID: 29977365 [PubMed] (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - July 8, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Maternal lipids, BMI and IL-17/IL-35 imbalance in concurrent gestational diabetes mellitus and preeclampsia.
In conclusion, the present study indicated that abnormal maternal lipids, hyperglycemia, high BMI, high CRP and IL-17/IL-35 imbalance may have a role in the pathophysiology of DPE. Therefore, pregnant women and clinicians should be made aware that maternal hyperlipidaemia, hyperglycemia, high BMI, high CRP levels and IL-17/IL-35 imbalance may lead to DPE. PMID: 29977366 [PubMed] (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - July 8, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Protective effect of Fructus corni polysaccharide on hippocampal tissues and its relevant mechanism in epileptic rats induced by lithium chloride-pilocarpine.
Authors: Sun X, Kong L, Zhou L Abstract The aim of the present study was to investigate the potential effect of Fructus corni polysaccharide (PFC) on the hippocampus tissues in epileptic rats induced by lithium chloride-pilocarpine, and to explore the underlying mechanism. The epileptic rat models were established using lithium chloride-pilocarpine treatment. According to the dosage of PFC, the rat models were divided into three groups: The low-dose (100 mg/kg/day), middle-dose (200 mg/kg/day) and high-dose (300 mg/kg/day) groups. The intervention for rat models lasted for 24 days. Subsequently, the production leve...
Source: Experimental and Therapeutic Medicine - July 8, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Relationship between 25-hydroxyvitamin D levels and inflammatory factors in children with asthma attack.
Authors: Wang S, Pan Y, Zhang Z Abstract The level of 25-hydroxyvitamin D [25-(OH)D] associated with inflammatory factors in children during an asthma attack was investigated. In total, 60 child patients, who were admitted and treated in the Affiliated Children's Hospital of Xuzhou Medical University from March 2015 to March 2017, during their asthma attacks, were selected as the observation group. The patients were divided into the high 25-(OH)D (n=28) and low 25-(OH)D (n=32) groups according to the median level of 25-(OH)D. A total of 30 healthy children were selected as the control group. Biochemical indexes, hu...
Source: Experimental and Therapeutic Medicine - June 16, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Rapamycin provides anti-epileptogenic effect in a rat model of post-traumatic epilepsy via deactivation of mTOR signaling pathway.
Authors: Wang F, Chen F, Wang G, Wei S, Fang F, Kang D, Lin Y Abstract The mammalian target of rapamycin (mTOR) signaling pathway has attracted much attention in recent years. However, the contribution of mTOR activation to the development of post-traumatic epilepsy (PTE) remains largely unknown. The purpose of the present study was to investigate the activation of mTOR signaling in a rat model of FeCl2-induced PTE, and to explore the potential effect of its specific inhibitor rapamycin. The results indicated that the expression levels of p-mTOR and p-P70S6K, the overactivation biomarkers of mTOR signaling, increas...
Source: Experimental and Therapeutic Medicine - June 16, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Comparative efficacy and tolerability of treatments for adult autoimmune hepatitis: A systematic review and network meta-analysis.
Authors: Lu FB, Hu ED, Xu LM, Hu YB, Chen L, Wu JL, Li H, Chen DZ, Chen YP Abstract The most suitable treatment regimen for autoimmune hepatitis (AIH) in adults remains unknown and requires further investigation. The current study therefore aimed to integrate evidence to provide hierarchies of the comparative efficacies of treatments measured by clinical and biochemical remission. A Bayesian-framework network meta-analysis of randomized controlled trials (RCTs) was preformed to compare eight treatments for AIH. Eligible RCTs were identified by searching Embase, Pubmed and the Cochrane Library for publications betwe...
Source: Experimental and Therapeutic Medicine - June 16, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Thymoquinone reduces spinal cord injury by inhibiting inflammatory response, oxidative stress and apoptosis via PPAR- γ and PI3K/Akt pathways.
Thymoquinone reduces spinal cord injury by inhibiting inflammatory response, oxidative stress and apoptosis via PPAR-γ and PI3K/Akt pathways. Exp Ther Med. 2018 Jun;15(6):4987-4994 Authors: Chen Y, Wang B, Zhao H Abstract The present study used a mild contusion injury in rat spinal cord to determine that thymoquinone reduces inflammatory response, oxidative stress and apoptosis in a spinal cord injury (SCI) rat model and to demonstrate its possible molecular mechanisms. The rats in the thymoquinone group received 30 mg/kg thymoquinone once daily by intragastric administration from 3 weeks after s...
Source: Experimental and Therapeutic Medicine - June 16, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Apoptosis and antimigration induction in human skin cancer cells by rhodomyrtone.
Authors: Tayeh M, Nilwarangkoon S, Tanunyutthawongse C, Mahabusarakum W, Watanapokasin R Abstract Rhodomyrtone is a bioactive compound extracted from Rhodomyrtus tomentosa leaves. It has been used as a traditional herb medicine for many years. Rhodomyrtone exhibits antibacterial activity, anti-inflammatory and antioxidant activities. However, the anticancer activity of rhodomyrtone has not been previously reported. The present study investigated the anticancer effect of rhomyrtone on human epidermoid carcinoma A431 cells. The cytotoxic and antiproliferative effects of rhodomyrtone on A431 cells were investigated by...
Source: Experimental and Therapeutic Medicine - June 16, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Icariin regulates the osteoblast differentiation and cell proliferation of MC3T3-E1 cells through microRNA-153 by targeting Runt-related transcription factor 2.
In conclusion, icariin exerted a strong osteoblast differentiation effect in MC3T3-E1 cells through the miR-153/Runx2 pathway. The current study provided evidence suggesting that icariin should be considered as an effective candidate for the management of osteoporosis. PMID: 29904399 [PubMed] (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - June 16, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Interactions between dendritic cells and T lymphocytes in pathogenesis of nasal polyps.
Authors: Lin X, Zhuang X, Li C, Wang X Abstract The aim of the present study was to investigate the functional status of dendritic cells (DCs) in nasal polyps (NP) and their interactions with T lymphocytes. The interactions between DC and T lymphocytes in the pathogenesis of NP was also studied. The expression of cluster of differentiation (CD)1a and CD83 in NP was detected using immunohistochemistry and the ratio of CD83 DC/CD1a+DC was counted. The distribution of DCs in NP and normal inferior turbinate mucosa (nITM) was evaluated using double immunostaining (CD1a/CD40) and low illumination fluorescence microscopy...
Source: Experimental and Therapeutic Medicine - June 16, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Analysis on the association between sperm DNA fragmentation index and conventional semen parameters, blood microelements and seminal plasma ROS in male patients with infertility.
Authors: Xie D, Lu C, Zhu Y, Zhu S, Yang EJ, Jin X Abstract We conducted this study to analyze the sperm DNA fragmentation index, conventional semen parameters, blood microelements and seminal plasma reactive oxygen species (ROS) in patients with male infertility to determine the association between each of the above male physiological parameters and DNA fragmentation index and infertility. Eighty cases of infertile males and 20 cases of normal males with children were divided into the infertility and control groups, respectively. Sperm DNA fragmentation index, conventional semen parameters, serum microelement cont...
Source: Experimental and Therapeutic Medicine - June 16, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Identification of CSF biomarkers by proteomics in Guillain-Barr é syndrome.
Identification of CSF biomarkers by proteomics in Guillain-Barré syndrome. Exp Ther Med. 2018 Jun;15(6):5177-5182 Authors: Li P, Wang S, Zhang R, Pei J, Chen L, Cao Y, Zhang H, Yang G Abstract The purpose of the present study was to screen for differentially expressed proteins in the cerebrospinal fluid (CSF) of patients with Guillain-Barré syndrome (GBS). The identification of differentially expressed protein can provide new targets for understanding the pathogenic mechanism, early clinical diagnosis, prognosis and for measuring the effectiveness of interventions. We enrolled 50 GBS pati...
Source: Experimental and Therapeutic Medicine - June 16, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Relationship of melatonin level, oxidative stress and inflammatory status with osteoporosis in maintenance hemodialysis of chronic renal failure.
Authors: Ren H, Sun R, Wang J Abstract The relationship of melatonin (MT) level, oxidative stress and inflammatory status with osteoporosis in the maintenance hemodialysis (MHD) of chronic renal failure was investigated. Ninety-four patients with chronic renal failure treated in Dezhou People's Hospital (Dezhoo, China) and receiving MHD for more than 3 months from May to April 2017 were selected and divided into the osteoporosis group (observation group, n=49) and the non-osteoporosis group (control group, n=45) based on whether osteoporosis was involved. The serum advanced oxidation protein products (AOPP), MT lev...
Source: Experimental and Therapeutic Medicine - June 16, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

MicroRNA-142-3p inhibits cell proliferation and chemoresistance in ovarian cancer via targeting sirtuin 1.
Authors: Gao J, Wu N, Liu X, Xia Y, Chen Y, Li S, Deng Z Abstract MicroRNAs (miRs) serve promoting or suppressive roles in various human cancer types, including ovarian cancer; however, the role of miR-142-3p in ovarian cancer growth and chemoresistance has not previously been studied. In the present study, reverse transcription-quantitative polymerase chain reaction and western blotting were used to examine miR and protein expression levels. An MTT assay was used to examine cell proliferation. A luciferase reporter gene assay was used to clarify the target gene of miR-142-3p. The present study reported that miR-14...
Source: Experimental and Therapeutic Medicine - June 16, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Predictive value of platelet aggregation rate in postpartum deep venous thrombosis and its possible mechanism.
Authors: Sun M, Liu C, Zhao N, Meng K, Zhang Z Abstract The present study investigated the predictive value of the platelet aggregation rate in postpartum deep venous thrombosis and its possible mechanism. From January 2014 to January 2016, 23 patients with postpartum deep vein thrombosis of lower extremity treated in the Department of Obstetrics of Beijing Chaoyang Hospital were as assigned as the observation group. At the same time, 25 cases with normal recovery were assigned as the control group. Blood samples were collected from all the subjects. The platelet aggregation rate was measured using a platelet aggre...
Source: Experimental and Therapeutic Medicine - June 16, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Systematic review and meta-analysis of the efficacy and safety of Biqi capsule in rheumatoid arthritis patients.
In conclusion, the present study indicated that compared with MTX, Biqi capsule plus MTX appeared to have more benefits but that Biqi capsule alone was not better for RA patients than MTX. In the other words, Biqi capsule plus MTX is more effective and has fewer AEs compared to MTX. However, the trials selected in the present meta-analysis have various limitations, including the lack of blinding and the short duration of the treatment; therefore, the conclusions are not sufficiently definitive. More randomized controlled trials are necessary to evaluate the use of Biqi capsule for managing RA. PMID: 29904406 [PubMed] (...
Source: Experimental and Therapeutic Medicine - June 16, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

The cardioprotective effects of secretory leukocyte protease inhibitor against myocardial ischemia/reperfusion injury.
Authors: Prompunt E, Sanit J, Barrère-Lemaire S, Nargeot J, Noordali H, Madhani M, Kumphune S Abstract Protease enzymes generated from injured cells and leukocytes are the primary cause of myocardial cell damage following ischemia/reperfusion (I/R). The inhibition of protease enzyme activity via the administration of particular drugs may reduce injury and potentially save patients' lives. The aim of the current study was to investigate the cardioprotective effects of treatment with recombinant human secretory leukocyte protease inhibitor (rhSLPI) on in vitro and ex vivo models of myocardial I/R injury. rhSLP...
Source: Experimental and Therapeutic Medicine - June 16, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

A novel dressing seeded with embryonic artery CD133+ cells and loaded with the Sirt1 agonist SRT1720 accelerates the healing of diabetic ischemic ulcers.
Authors: Cheng PK, Chen XL, Su XX, Su XJ, Hou CL Abstract Refractory ischemic ulcers that occur in patients with diabetes present a major clinical challenge. Embryonic artery cluster of differentiation 133+ cells (EACCs) may promote the healing of diabetic ulcers; however, the high glucose environment in the diabetic ulcers decreases the survival rate of transplanted EACCs and inhibit their biological function. Furthermore, microcirculation in diabetic ischemic ulcers is impaired, which inhibits the beneficial effect of EACCs. In the current study, the Sirt1 agonist SRT1720 was selected as a therapeutic drug and lo...
Source: Experimental and Therapeutic Medicine - June 16, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Effects of HSYA on the proliferation and apoptosis of MSCs exposed to hypoxic and serum deprivation conditions.
In conclusion, the results of the current study demonstrated that HSYA exerts protective effects against H/SD-induced apoptosis in MSCs potentially via activation of the HIF-1α/VEGF signaling pathway and stabilization of the mitochondrial membrane. PMID: 29904409 [PubMed] (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - June 16, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Influencing factors of NT-proBNP level inheart failure patients with different cardiacfunctions and correlation with prognosis.
Authors: Xu L, Chen Y, Ji Y, Yang S Abstract Factors influencing N-terminal pro-brain natriuretic peptide (NT-proBNP) level in heart failure patients with different cardiac functions were identified to explore the correlations with prognosis. Eighty heart failure patients with different cardiac functions treated in Yixing People's Hospital from January 2016 to June 2017 were selected, and divided into two groups (group with cardiac function in class II and below and group with cardiac function in class III and above), according to the cardiac function classification established by New York Heart Association (NYHA)....
Source: Experimental and Therapeutic Medicine - June 16, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Efficacy of Toric intraocular lens implantation in eyes with high myopia: A prospective, case-controlled observational study.
Authors: Guo T, Gao P, Fang L, Guo L, Fan Y, Liu C Abstract The present prospective clinical observational study aimed to evaluate the efficacy of Toric intraocular lenses (IOL) to achieve rotational stability and astigmatism correction in eyes with high myopia. A total of 27 consecutive cataract patients (39 eyes) with pre-existing corneal astigmatism (1.5-3.5 D) were divided into two groups according to their refractive status: One group of 18 eyes with high myopia -(12.5-6.0 D) and another group consisting of 21 eyes with emmetropia or low myopia (-3.0-0.0 D). All eyes underwent cataract phacoemulsification surg...
Source: Experimental and Therapeutic Medicine - June 16, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Spontaneous remission of obstructive jaundice in rats: Selection of experimental models.
Authors: Lv Y, Yue J, Gong X, Han X, Wu H, Deng J, Li Y Abstract The aim of the present study was to evaluate the prevalence and causes of spontaneous remission of obstructive jaundice in rats. Healthy male and female Wistar rats (180-220 g) were randomly assigned to receive common bile duct ligation (CBDL) and transection (group A), CBDL only (group B), or CBD dissection without ligation or transection (control group C; n=36 in each group). There was a difference in eye and skin jaundice prevalence between groups A and B from 14 days after surgery. The level of total bilirubin (TB) did not continue to increase in ...
Source: Experimental and Therapeutic Medicine - June 16, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Minimally effective concentration of zoledronic acid to suppress osteoclasts in vitro.
In conclusion, zoledronic acid was effective in reducing osteoclast formation and suppressing cellular functions. The minimal effective concentration of zoledronic acid in vitro was 1 µmol/l. Based on these results, a comparable dosage should be explored in clinical applications. PMID: 29904413 [PubMed] (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - June 16, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

PYNOD reduces microglial inflammation and consequent neurotoxicity upon lipopolysaccharides stimulation.
In conclusion, PYNOD may mitigate microglial inflammation and consequent neurotoxicity. PMID: 29904414 [PubMed] (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - June 16, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

TGF- β1 combined with Sal-B promotes cardiomyocyte differentiation of rat mesenchymal stem cells.
TGF-β1 combined with Sal-B promotes cardiomyocyte differentiation of rat mesenchymal stem cells. Exp Ther Med. 2018 Jun;15(6):5359-5364 Authors: Lv Y, Liu B, Liu Y, Wang H, Wang H Abstract Transforming growth factor β1 (TGF-β1) and salvianolic acid B (Sal-B) are key signaling factors for stem cell differentiation into cardiomyocytes (CMs). The present study compared the biological effect of TGF-β1 and Sal-B, alone or in combination, on bone marrow mesenchymal stromal cells (BMSCs) that differentiate into myocardial-like cells in a simulated myocardial microenvironment in vitro. BMSC...
Source: Experimental and Therapeutic Medicine - June 16, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Experimental study of epidermal growth factor and acidic fibroblast growth factor in the treatment of diabetic foot wounds.
Authors: Xu J, Min D, Guo G, Liao X, Fu Z Abstract The aim of the present study was to investigate the effect of epidermal growth factor (EGF) and acidic fibroblast growth factor (aFGF) on the healing of diabetic foot wounds. A total of 199 patients with diabetic foot ulcers were recruited and randomly divided into four groups: A recombinant human EGF group (n=50), an aFGF group (n=50), a combined EGF and aFGF group (n=50) and a normal saline control group (n=49). Patients in all groups received a daily dressing change and growth factor reagents were applied topically when dressing. To observe the time required for...
Source: Experimental and Therapeutic Medicine - June 16, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Observation of corneal transplantation in peripheral corneal disease postoperatively.
Authors: Zhang Y, Hu Y, Li X, Shi X, Xu F, Jia H Abstract The aim of the present study was to investigate the role of the limbal stem cells in corneal epithelial homeostasis in patients with peripheral corneal disease who received corneal transplantation surgery. This retrospective study enrolled 85 patients (85 eyes) with peripheral corneal lesion who underwent corneal transplantation at Department of Ophthalmology in First Hospital of Jilin University, Jilin, China. All patients during each follow-up period were examined for best corrected visual acuity, corneal reconstruction (assessed by slit-lamp biomicroscopy...
Source: Experimental and Therapeutic Medicine - June 16, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Acquired hypophosphatemic osteomalacia is easily misdiagnosed or neglected by rheumatologists: A report of 9 cases.
In conclusion, diagnosing HO is challenging for rheumatologists and physicians. Basic examinations of electrolyte balance and bone mineral density should be performed, as should tumor screening and a careful collection of patient medical history and drugs in young patients with unexplained thoracic or back pain and muscle weakness. Removing any secondary etiology, such as drugs may dramatically improve the patients clinical manifestations and result in an improved prognosis. PMID: 29904418 [PubMed] (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - June 16, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Value of procalcitonin for diagnosing perioperative pneumonia, urinary infections and superficial surgical site infections in patients undergoing primary hip and knee arthroplasty.
Authors: Zhang L, Cai D, Guo H Abstract Serum procalcitonin (PCT) levels may be helpful for the diagnosis of infections during the perioperative period of arthroplasty. However, there is much debate in the literature regarding the appropriate cut-off value for different types of surgery and local bacterial infection. The present study aimed to evaluate serum PCT levels in patients undergoing arthroplasty and to determine the cut-off value that may represent perioperative pneumonia, urinary infections and superficial surgical site infections. The present retrospective study included a total of 500 patients treated b...
Source: Experimental and Therapeutic Medicine - June 16, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Analysis of the efficacy of resveratrol treatment in patients with scarred uterus.
In conclusion, the findings of the current study indicate that resveratrol treatment may be a potential strategy for the treatment of scarred uterus patients, which contributes to the improvement of pregnancy outcomes. PMID: 29904420 [PubMed] (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - June 16, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Contrast-enhanced magnetic resonance imaging with a novel nano-size contrast agent for the clinical diagnosis of patients with lung cancer.
Authors: Gao J, Li L, Liu X, Guo R, Zhao B Abstract Recent studies have indicated that magnetic resonance imaging (MRI) efficiently diagnoses lung cancer. However, the efficacy of MRI in diagnosing lung cancer requires improving for patients in the early stage of the disease. In the present study, a novel nano-sized contrast agent of chistosan/Fe3O4-enclosed bispecific antibodies (BsAbCENS) was introduced, which targeted carcino-embryonic antigen (CEA) and neuron-specific enolase (NSE) in lung cancer cells. The diagnostic efficacy of contrast-enhanced MRI with BsAbCENS (CEMRI-BsAbCENS) was investigated in a total o...
Source: Experimental and Therapeutic Medicine - June 16, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Prolyl oligopeptidase inhibitor suppresses the upregulation of ACSDKP in patients with acute myeloid leukemia.
Authors: Cao H, Zhao X, Lu S, Wang Z Abstract The aim of the current study was to measure the expression of acetyl-N-Ser-Asp-Lys-Pro (ACSDKP) in patients with acute myeloid leukemia (AML) and the effect of prolyl oligopeptidase inhibitor (POPi) on the bone marrow stromal cells of these patients. Serum and bone marrow stromal cell samples were collected from 33 patients with AML admitted to Wuxi Second People's Hospital, Nanjing Medical University between September 2011 and August 2016. ACSDKP levels were measured using a highly specific competitive enzyme immunoassay (EIA). Bone marrow stromal cells were treated wi...
Source: Experimental and Therapeutic Medicine - June 16, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

The NF κB signaling pathway serves an important regulatory role in Klebsiella pneumoniae liver abscesses.
The NFκB signaling pathway serves an important regulatory role in Klebsiella pneumoniae liver abscesses. Exp Ther Med. 2018 Jun;15(6):5443-5449 Authors: Zhang M, Pan L, Xu D, Cao C, Shi R, Han S, Liu J, Li X, Li M Abstract The incidence of Klebsiella pneumoniae liver abscess (KPLA) has increased in a number of Asian countries over the past 30 years. Diabetes mellitus (DM) is a risk factor for KPLA. The prevalence and clinical features of KPLA in patients with and without DM have been well described; however, the underlying molecular mechanism responsible for the increased incidence of KPLA in pat...
Source: Experimental and Therapeutic Medicine - June 16, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Expression and role of VEGFA and miR-381 in portal vein tumor thrombi in patients with hepatocellular carcinoma.
Authors: Wang J, Wu S, Huang T Abstract The aim of the present study was to examine the expression and role of vascular endothelial growth factor A (VEGFA) and microRNA (miRNA or miR)-381 in tumor thrombi from patients with hepatocellular carcinoma and portal vein tumor thrombus (PVTT). Tumor thrombi and adjacent paired tissues were collected from 39 patients with hepatocellular carcinoma with PVTT. VEGFA expression levels were assessed using reverse transcription-quantitative polymerase chain reaction and western blotting. miRNAs that may regulate VEGFA expression were predicted using bioinformatics analysis and c...
Source: Experimental and Therapeutic Medicine - June 16, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Analysis of clinical risk factors in relapsed patients with class IV lupus nephritis.
Authors: Wang J, Qi YY, Chen XP, Ma L, Zhang LL, Zhao Y, Wang M Abstract The present study aimed to investigate risk factors for renal recurrence in patients with type IV lupus nephritis (LN). Univariate and multivariate analyses were conducted to using the Cox proportional hazard model and the Kaplan-Meier method. A total 244 patients were diagnosed with type IV LN; 100 (28.49%) relapsed and 144 (41.03%) recovered successfully. Kaplan-Meier method analysis indicated that patients with type IV LN affecting the digestive tract had high renal recurrence rates. Patients with hyperglobulinemia, positive anti-ribonucleo...
Source: Experimental and Therapeutic Medicine - June 16, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Antinociceptive effects of dezocine on complete Freund's adjuvant-induced inflammatory pain in rats.
In conclusion, the analgesic effect of dezocine on inflammatory pain induced by CFA may be associated with the inhibition of the spinal ERK1/2-COX-2 pathway. PMID: 29904426 [PubMed] (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - June 16, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Association of serum interleukin-10, interleukin-17A and transforming growth factor- α levels with human benign and malignant breast diseases.
Association of serum interleukin-10, interleukin-17A and transforming growth factor-α levels with human benign and malignant breast diseases. Exp Ther Med. 2018 Jun;15(6):5475-5480 Authors: Lv Z, Liu M, Shen J, Xiang D, Ma Y, Ji Y Abstract Interleukin-10 (IL-10), interleukin-17A (IL-17A) and transforming growth factor α (TGF-α) have been implicated in the progression of breast cancer. However, the diagnostic and prognostic roles of these cytokines in ductal carcinoma remain unclear. The present study therefore aimed to determine the serum levels of IL-10, IL-17 and TGF-α in subj...
Source: Experimental and Therapeutic Medicine - June 16, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Flexible bronchoscopy in pulmonary diseases in children with congenital cardiovascular abnormalities.
Authors: Chen T, Qiu L, Zhong L, Tao Q, Liu H, Chen L Abstract There is a lack of data describing the role of flexible bronchoscopy (FB) in evaluating pulmonary diseases in children with congenital cardiovascular abnormalities in China. Determining whether those children suffer from respiratory complications may be useful for future preoperative planning and family counseling. The present study aimed to investigate the features and FB-associated findings with respect to pulmonary diseases in children with congenital cardiovascular abnormalities. The role of FB in guiding the treatment and safety of procedures was a...
Source: Experimental and Therapeutic Medicine - June 16, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Effect of dexmedetomidine, midazolam, and propofol on lipopolysaccharide-stimulated dendritic cells.
Authors: Guo F, Ding Y, Yu X, Cai X Abstract Dexmedetomidine, midazolam and propofol are common sedative drugs used in the intensive care unit. Lipopolysaccharides (LPS) are a potent inducer of human dendritic cells (DCs) maturation and survival, which induces cytokine production. The present study aimed to investigate the effect and mechanisms of sedative drugs on LPS-induced cytokine production in DCs. The mouse bone marrow-derived dendritic DC2.4 cell line was used in the present study. The Cell Counting Kit-8 assay was used to measure the viability of cells. Tumor necrosis factor (TNF)-α, interleukin (IL)...
Source: Experimental and Therapeutic Medicine - June 16, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research