Moxibustion alleviates chronic heart failure by regulating mitochondrial dynamics and inhibiting autophagy
Exp Ther Med. 2022 May;23(5):359. doi: 10.3892/etm.2022.11286. Epub 2022 Mar 30.ABSTRACTMoxibustion (MOX) is a traditional Chinese medicine preparation, which has been clinically used to treat cardiac diseases in recent years. The present study aimed to examine the protective effects and possible mechanisms of MOX on doxorubicin (DOX)-induced chronic heart failure (CHF) in rats. The animals were divided into five groups, including the Control (normal saline), DOX (doxorubicin 15 mg/kg), MOX (doxorubicin 15 mg/kg + moxibustion), BEN (doxorubicin 15 mg/kg + benazepril 0.86 mg/kg) and MOX + BEN (doxorubicin 15 mg/kg + moxibus...
Source: Experimental and Therapeutic Medicine - May 2, 2022 Category: General Medicine Authors: Ran Xia Wei Wang Bing Gao Qiang Ma Jing Wang Xiaohua Dai Qingling Li Source Type: research

Sinensetin suppresses angiogenesis in liver cancer by targeting the VEGF/VEGFR2/AKT signaling pathway
Exp Ther Med. 2022 May;23(5):360. doi: 10.3892/etm.2022.11287. Epub 2022 Mar 31.ABSTRACTSinensetin (SIN) is a polymethoxy flavone primarily present in citrus fruits. This compound has demonstrated anticancer activity. However, the underlying mechanism of its action has not been fully understood. The present study investigated the impact of SIN on angiogenesis in a liver cancer model. In a murine xenograft tumor model, SIN inhibited the growth of HepG2/C3A human liver hepatoma cell-derived tumors and reduced the expression levels of platelet/endothelial cell adhesion molecule-1 and VEGF. In HepG2/C3A cells, SIN repressed VE...
Source: Experimental and Therapeutic Medicine - May 2, 2022 Category: General Medicine Authors: Xiao Li Yan Li Yuan Wang Fuhong Liu Yanjun Liu Jiangjiu Liang Rucai Zhan Yue Wu He Ren Xiuyuan Zhang Ju Liu Source Type: research

Balanitis xerotica obliterans: An observational, descriptive and retrospective clinical study
Exp Ther Med. 2022 May;23(5):361. doi: 10.3892/etm.2022.11288. Epub 2022 Mar 31.ABSTRACTLichen sclerosus is a chronic disease of unknown etiology that can occur in the genital area of both sexes. Balanitis xerotica obliterans (BXO) occurs only in the genital area of men and typically affects the foreskin, penile glans, and/or the meatus. In the present report, an observational, descriptive, and retrospective epidemiological clinical study of available data between January 2006 and December 2020 in patients diagnosed with BXO from the casuistic of CMI DERMAMED (Târgu Mureș, Romania) was performed. During this 15-year peri...
Source: Experimental and Therapeutic Medicine - May 2, 2022 Category: General Medicine Authors: Gyula L ászló Fekete Dominik Schwarzkopf-Kolb Ilarie Brihan Daniel Boda L ászló Fekete Source Type: research

miR-148a-3p facilitates osteogenic differentiation of fibroblasts in ankylosing spondylitis by activating the Wnt pathway and targeting DKK1
Exp Ther Med. 2022 May;23(5):365. doi: 10.3892/etm.2022.11292. Epub 2022 Apr 1.ABSTRACTAnkylosing spondylitis (AS) is a chronic inflammatory form of arthritis. MicroRNAs (miRNAs) have been identified to serve as therapeutic targets in various inflammatory diseases. The aim of the present study was to determine the functional mechanism of miR-148a-3p on AS. Specimens were collected from AS patients and non-AS patients. Fibroblasts were delivered with the aid of miR-148a-3p inhibitor. Cell staining was performed to observe the morphological changes, calcified nodules, and mineralization degree. The binding sites of miR-148a-...
Source: Experimental and Therapeutic Medicine - May 2, 2022 Category: General Medicine Authors: Wenbo Sheng Haitao Jiang Hantao Yuan Sibo Li Source Type: research

Relationship of interferon regulator factor 5 and interferon- γ with missed abortion
Exp Ther Med. 2022 May;23(5):356. doi: 10.3892/etm.2022.11283. Epub 2022 Mar 29.ABSTRACTThe aim of the present study was to reveal the association of missed abortion, a process integrated with the immune system, with interferon regulatory factor 5 (IRF5) and interferon-γ (IFN-γ), and to demonstrate the function of these molecules by examining their levels in decidual tissue. This prospective cohort study included 13 patients with no additional systemic disease, between 6 and 10 weeks of gestation with negative fetal heartbeat, and 11 patients between 6 and 10 weeks of gestation with positive heartbeat who presented for v...
Source: Experimental and Therapeutic Medicine - May 2, 2022 Category: General Medicine Authors: Dilsad Herkiloglu Sefik Gokce Ozge Cevik Source Type: research

miR-223 ameliorates thalamus hemorrhage-induced central poststroke pain via targeting NLRP3 in a mouse model
In conclusion, these results may determine the mechanisms underlying CPSP and facilitate development of targeted therapy for CPSP.PMID:35493427 | PMC:PMC9019782 | DOI:10.3892/etm.2022.11280 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - May 2, 2022 Category: General Medicine Authors: Tianfeng Huang Yinggang Xiao Yang Zhang Cunjin Wang Xiaoping Chen Yong Li Yali Ge Ju Gao Source Type: research

Leonurine: A compound with the potential to prevent acute lung injury
Exp Ther Med. 2022 May;23(5):358. doi: 10.3892/etm.2022.11285. Epub 2022 Mar 29.ABSTRACTSepsis is an intense immune response to infection that contributes to the pathophysiological process of acute lung injury (ALI). Inflammation and oxidative stress serve an important role in the development of ALI. Leonurine (LEO) is a natural phenolic alkaloid extracted from Leonurus cardiaca, which possesses anti-inflammatory and antioxidative properties. Therefore, the aim of the present study was to explore the effect of LEO on sepsis-induced ALI and to investigate its underlying mechanism. MTT and Cell Counting Kit-8 assays were per...
Source: Experimental and Therapeutic Medicine - May 2, 2022 Category: General Medicine Authors: Guoying Zhang Lanfei Wang Source Type: research

Seminal plasma piRNA array analysis and identification of possible biomarker piRNAs for the diagnosis of asthenozoospermia
Exp Ther Med. 2022 May;23(5):347. doi: 10.3892/etm.2022.11275. Epub 2022 Mar 23.ABSTRACTAsthenozoospermia (AZS) is characterized by reduced sperm motility and its pathogenesis remains poorly understood. Piwi-interacting RNAs (piRNAs) have been indicated to serve important roles in spermatogenesis. However, little is known about the correlation of piRNA expression with AZS. In the present study, small RNA sequencing (small RNA-seq) was performed on sperm samples from AZS patients and fertile controls. Reverse transcription-quantitative (RT-q) PCR was used to validate the small RNA-seq results. Bioinformatics analyses were p...
Source: Experimental and Therapeutic Medicine - May 2, 2022 Category: General Medicine Authors: Ling He Xingwu Wu Rongye Wu Ping Guo Wenfeng He Wanlei Sun He Chen Source Type: research

Regulation of apoptosis, autophagy and ferroptosis by non-coding RNAs in metastatic non-small cell lung cancer (Review)
Exp Ther Med. 2022 May;23(5):352. doi: 10.3892/etm.2022.11279. Epub 2022 Mar 28.ABSTRACTNon-small cell lung cancer (NSCLC), a common type of cancer worldwide, is normally associated with a poor prognosis. It is difficult to treat successfully as it often metastasizes into brain or bone. Methods to facilitate the induction of effective programmed cell death (PCD) in NSCLC cells to reverse drug resistance, or to inhibit the invasion and migration of NSCLC cells, are currently under investigation. The present study summarized the regulatory functions of PCD, including apoptosis, autophagy and ferroptosis, in the context of NS...
Source: Experimental and Therapeutic Medicine - May 2, 2022 Category: General Medicine Authors: Lei Xu Xin Huang Yan Lou Wei Xie Hangyu Zhao Source Type: research

Long COVID and neuropsychiatric manifestations (Review)
Exp Ther Med. 2022 May;23(5):363. doi: 10.3892/etm.2022.11290. Epub 2022 Apr 1.ABSTRACTThere is accumulating evidence in the literature indicating that a number of patients with coronavirus disease 2019 (COVID-19) may experience a range of neuropsychiatric symptoms, persisting or even presenting following the resolution of acute COVID-19. Among the neuropsychiatric manifestations more frequently associated with 'long COVID' are depression, anxiety, post-traumatic stress disorder, sleep disturbances, fatigue and cognitive deficits, that can potentially be debilitating and negatively affect patients' wellbeing, albeit in the...
Source: Experimental and Therapeutic Medicine - May 2, 2022 Category: General Medicine Authors: Vasiliki Efstathiou Maria-Ioanna Stefanou Marina Demetriou Nikolaos Siafakas Michael Makris Georgios Tsivgoulis Vassilios Zoumpourlis Stylianos P Kympouropoulos James N Tsoporis Demetrios A Spandidos Nikolaos Smyrnis Emmanouil Rizos Source Type: research

Patterns of indolence in prostate cancer (Review)
Exp Ther Med. 2022 May;23(5):351. doi: 10.3892/etm.2022.11278. Epub 2022 Mar 28.ABSTRACTAlthough prostate cancer is a major cause of cancer-related mortality worldwide, most patients will have a relatively indolent clinical course. Contrary to most other types of cancer, even the diagnosis of locally advanced or metastatic disease is not always lethal. The present review aimed to summarize what is known regarding the underlying mechanisms related to the indolent course of subsets of prostate cancer, at various stages. The data suggested that no specific gene alteration by itself was responsible for carcinogenesis or diseas...
Source: Experimental and Therapeutic Medicine - May 2, 2022 Category: General Medicine Authors: Minas Sakellakis Laura Jacqueline Flores Sumankalai Ramachandran Source Type: research

Vitamin D3 alleviates cigarette smoke extract-mediated epithelial-mesenchymal transition and fibrogenesis by upregulating CC16 expression in bronchial epithelial cells
Exp Ther Med. 2022 May;23(5):357. doi: 10.3892/etm.2022.11284. Epub 2022 Mar 29.ABSTRACTVitamin D3 supplementation has been previously reported to inhibit the occurrence and development of chronic obstructive pulmonary disease (COPD). However, the underlying mechanism remains unclear. Epithelial-mesenchymal transition (EMT) and fibrogenesis have been associated with the development of COPD. The aim of the present study was to investigate the potential effects and mechanism of vitamin D3 in an in vitro model of cigarette smoke (CS)-induced EMT and fibrosis, with specific focus on the role of club cell protein 16 (CC16). CS ...
Source: Experimental and Therapeutic Medicine - May 2, 2022 Category: General Medicine Authors: Yajun Mao Hong Feng Source Type: research

Serum levels of angiopoietin 2 mRNA in the mortality outcome prediction of septic shock
Exp Ther Med. 2022 May;23(5):362. doi: 10.3892/etm.2022.11289. Epub 2022 Apr 1.ABSTRACTIt has been reported that angiopoietin 2 (Ang-2) plays an integral role in the pathophysiology of sepsis and many other inflammatory diseases. However, the specific role of Ang-2 in septic shock has not been defined. The aim of the present study was to assess the predictive value of serum Ang-2 in patients with septic shock. Clinical data of 85 patients with septic shock and 10 healthy controls admitted to the intensive care unit with a diagnosis of septic shock were collected between January 2020 and October 2020 at Tongji Hospital (Wuh...
Source: Experimental and Therapeutic Medicine - May 2, 2022 Category: General Medicine Authors: Jun Feng Lili Wang Yikuan Feng Gang Yu Daixing Zhou Junshuai Wang Source Type: research

A combination of two regional flaps for the complex reconstruction after head and neck cancer surgery as an alternative approach during the COVID-19 pandemic: A case report
Exp Ther Med. 2022 May;23(5):364. doi: 10.3892/etm.2022.11291. Epub 2022 Apr 1.ABSTRACTSince the COVID-19 pandemic outbreak, the medical systems were challenged by continuously increasing numbers of infections and faced critical issues when trying to find solutions for patients suffering from other diseases, including patients with head and neck cancers. Complex surgeries were delayed due to an acute deficit of specialized intensive care medical staff and equipment, which were redirected towards COVID-19 hospitalized cases, with irreversible consequences for the patients. In the present study, the case of locally advanced ...
Source: Experimental and Therapeutic Medicine - May 2, 2022 Category: General Medicine Authors: Ana Caruntu Liliana Moraru Raluca Monica Comaneanu Raluca Simona Costache Titus Alexandru Farcasiu Cristian Scheau Daniel Octavian Costache Constantin Caruntu Source Type: research

CD47 antibody protects mice from doxorubicin-induced myocardial damage by suppressing cardiomyocyte apoptosis
Exp Ther Med. 2022 May;23(5):350. doi: 10.3892/etm.2022.11277. Epub 2022 Mar 24.ABSTRACTCluster of differentiation 47 (CD47) is upregulated in mouse models of doxorubicin (Dox)-induced dilated cardiomyopathy (DCM). To explore the role of CD47 in the development of DCM, in the present study, CD47 signaling was blocked by an anti-CD47 neutralizing antibody (aCD47) in mice with Dox-induced DCM. Intraperitoneal (i.p.) administration of 10 mg/kg Dox once a week significantly induced the development of DCM after 4 weeks, which was accompanied by the upregulation of CD47 expression in heart tissues. However, co-administration of ...
Source: Experimental and Therapeutic Medicine - May 2, 2022 Category: General Medicine Authors: Yan Hao Lianghua Chen Zhilong Jiang Source Type: research