Challenges of phototherapy for neonatal hyperbilirubinemia (Review)
Exp Ther Med. 2021 Mar;21(3):231. doi: 10.3892/etm.2021.9662. Epub 2021 Jan 20.ABSTRACTPhototherapy is universally recognized as the first option for treating neonatal jaundice due to its unparalleled efficiency and safety in reducing the high serum free bilirubin levels and limiting its neurotoxic effects. However, several studies have suggested that phototherapy may elicit a series of short- and long-term adverse reactions associated with pediatric diseases, including hemolysis, allergic diseases, DNA damage or even cancer. The aim of the present review was to summarize the etiology, mechanism, associated risks and thera...
Source: Experimental and Therapeutic Medicine - February 22, 2021 Category: General Medicine Authors: Juan Wang Genxin Guo Aimin Li Wen-Qi Cai Xianwang Wang Source Type: research

4-Methoxybenzylalcohol protects brain microvascular endothelial cells against oxygen-glucose deprivation/reperfusion-induced injury via activation of the PI3K/AKT signaling pathway
Exp Ther Med. 2021 Mar;21(3):252. doi: 10.3892/etm.2021.9684. Epub 2021 Jan 24.ABSTRACTDamage to the blood-brain barrier (BBB) during the process of cerebral ischemic injury is a key factor that affects the treatment of this condition. The present study aimed to assess the potential effects of 4-methoxybenzyl alcohol (4-MA) on brain microvascular endothelial cells (bEnd.3) against oxygen-glucose deprivation/reperfusion (OGD/Rep) using an in vitro model that mimics in vivo ischemia/reperfusion injury. In addition, the present study aimed to explore whether this underlying mechanism was associated with the inhibition of pro-...
Source: Experimental and Therapeutic Medicine - February 22, 2021 Category: General Medicine Authors: Qing Lin Weili Wang Liping Yang Xiaohua Duan Source Type: research

Challenges of phototherapy for neonatal hyperbilirubinemia (Review)
Exp Ther Med. 2021 Mar;21(3):231. doi: 10.3892/etm.2021.9662. Epub 2021 Jan 20.ABSTRACTPhototherapy is universally recognized as the first option for treating neonatal jaundice due to its unparalleled efficiency and safety in reducing the high serum free bilirubin levels and limiting its neurotoxic effects. However, several studies have suggested that phototherapy may elicit a series of short- and long-term adverse reactions associated with pediatric diseases, including hemolysis, allergic diseases, DNA damage or even cancer. The aim of the present review was to summarize the etiology, mechanism, associated risks and thera...
Source: Experimental and Therapeutic Medicine - February 22, 2021 Category: General Medicine Authors: Juan Wang Genxin Guo Aimin Li Wen-Qi Cai Xianwang Wang Source Type: research

4-Methoxybenzylalcohol protects brain microvascular endothelial cells against oxygen-glucose deprivation/reperfusion-induced injury via activation of the PI3K/AKT signaling pathway
Exp Ther Med. 2021 Mar;21(3):252. doi: 10.3892/etm.2021.9684. Epub 2021 Jan 24.ABSTRACTDamage to the blood-brain barrier (BBB) during the process of cerebral ischemic injury is a key factor that affects the treatment of this condition. The present study aimed to assess the potential effects of 4-methoxybenzyl alcohol (4-MA) on brain microvascular endothelial cells (bEnd.3) against oxygen-glucose deprivation/reperfusion (OGD/Rep) using an in vitro model that mimics in vivo ischemia/reperfusion injury. In addition, the present study aimed to explore whether this underlying mechanism was associated with the inhibition of pro-...
Source: Experimental and Therapeutic Medicine - February 22, 2021 Category: General Medicine Authors: Qing Lin Weili Wang Liping Yang Xiaohua Duan Source Type: research

Challenges of phototherapy for neonatal hyperbilirubinemia (Review)
Exp Ther Med. 2021 Mar;21(3):231. doi: 10.3892/etm.2021.9662. Epub 2021 Jan 20.ABSTRACTPhototherapy is universally recognized as the first option for treating neonatal jaundice due to its unparalleled efficiency and safety in reducing the high serum free bilirubin levels and limiting its neurotoxic effects. However, several studies have suggested that phototherapy may elicit a series of short- and long-term adverse reactions associated with pediatric diseases, including hemolysis, allergic diseases, DNA damage or even cancer. The aim of the present review was to summarize the etiology, mechanism, associated risks and thera...
Source: Experimental and Therapeutic Medicine - February 22, 2021 Category: General Medicine Authors: Juan Wang Genxin Guo Aimin Li Wen-Qi Cai Xianwang Wang Source Type: research

4-Methoxybenzylalcohol protects brain microvascular endothelial cells against oxygen-glucose deprivation/reperfusion-induced injury via activation of the PI3K/AKT signaling pathway
Exp Ther Med. 2021 Mar;21(3):252. doi: 10.3892/etm.2021.9684. Epub 2021 Jan 24.ABSTRACTDamage to the blood-brain barrier (BBB) during the process of cerebral ischemic injury is a key factor that affects the treatment of this condition. The present study aimed to assess the potential effects of 4-methoxybenzyl alcohol (4-MA) on brain microvascular endothelial cells (bEnd.3) against oxygen-glucose deprivation/reperfusion (OGD/Rep) using an in vitro model that mimics in vivo ischemia/reperfusion injury. In addition, the present study aimed to explore whether this underlying mechanism was associated with the inhibition of pro-...
Source: Experimental and Therapeutic Medicine - February 22, 2021 Category: General Medicine Authors: Qing Lin Weili Wang Liping Yang Xiaohua Duan Source Type: research

Challenges of phototherapy for neonatal hyperbilirubinemia (Review)
Exp Ther Med. 2021 Mar;21(3):231. doi: 10.3892/etm.2021.9662. Epub 2021 Jan 20.ABSTRACTPhototherapy is universally recognized as the first option for treating neonatal jaundice due to its unparalleled efficiency and safety in reducing the high serum free bilirubin levels and limiting its neurotoxic effects. However, several studies have suggested that phototherapy may elicit a series of short- and long-term adverse reactions associated with pediatric diseases, including hemolysis, allergic diseases, DNA damage or even cancer. The aim of the present review was to summarize the etiology, mechanism, associated risks and thera...
Source: Experimental and Therapeutic Medicine - February 22, 2021 Category: General Medicine Authors: Juan Wang Genxin Guo Aimin Li Wen-Qi Cai Xianwang Wang Source Type: research

4-Methoxybenzylalcohol protects brain microvascular endothelial cells against oxygen-glucose deprivation/reperfusion-induced injury via activation of the PI3K/AKT signaling pathway
Exp Ther Med. 2021 Mar;21(3):252. doi: 10.3892/etm.2021.9684. Epub 2021 Jan 24.ABSTRACTDamage to the blood-brain barrier (BBB) during the process of cerebral ischemic injury is a key factor that affects the treatment of this condition. The present study aimed to assess the potential effects of 4-methoxybenzyl alcohol (4-MA) on brain microvascular endothelial cells (bEnd.3) against oxygen-glucose deprivation/reperfusion (OGD/Rep) using an in vitro model that mimics in vivo ischemia/reperfusion injury. In addition, the present study aimed to explore whether this underlying mechanism was associated with the inhibition of pro-...
Source: Experimental and Therapeutic Medicine - February 22, 2021 Category: General Medicine Authors: Qing Lin Weili Wang Liping Yang Xiaohua Duan Source Type: research

Challenges of phototherapy for neonatal hyperbilirubinemia (Review)
Exp Ther Med. 2021 Mar;21(3):231. doi: 10.3892/etm.2021.9662. Epub 2021 Jan 20.ABSTRACTPhototherapy is universally recognized as the first option for treating neonatal jaundice due to its unparalleled efficiency and safety in reducing the high serum free bilirubin levels and limiting its neurotoxic effects. However, several studies have suggested that phototherapy may elicit a series of short- and long-term adverse reactions associated with pediatric diseases, including hemolysis, allergic diseases, DNA damage or even cancer. The aim of the present review was to summarize the etiology, mechanism, associated risks and thera...
Source: Experimental and Therapeutic Medicine - February 22, 2021 Category: General Medicine Authors: Juan Wang Genxin Guo Aimin Li Wen-Qi Cai Xianwang Wang Source Type: research

4-Methoxybenzylalcohol protects brain microvascular endothelial cells against oxygen-glucose deprivation/reperfusion-induced injury via activation of the PI3K/AKT signaling pathway
Exp Ther Med. 2021 Mar;21(3):252. doi: 10.3892/etm.2021.9684. Epub 2021 Jan 24.ABSTRACTDamage to the blood-brain barrier (BBB) during the process of cerebral ischemic injury is a key factor that affects the treatment of this condition. The present study aimed to assess the potential effects of 4-methoxybenzyl alcohol (4-MA) on brain microvascular endothelial cells (bEnd.3) against oxygen-glucose deprivation/reperfusion (OGD/Rep) using an in vitro model that mimics in vivo ischemia/reperfusion injury. In addition, the present study aimed to explore whether this underlying mechanism was associated with the inhibition of pro-...
Source: Experimental and Therapeutic Medicine - February 22, 2021 Category: General Medicine Authors: Qing Lin Weili Wang Liping Yang Xiaohua Duan Source Type: research

Challenges of phototherapy for neonatal hyperbilirubinemia (Review)
Exp Ther Med. 2021 Mar;21(3):231. doi: 10.3892/etm.2021.9662. Epub 2021 Jan 20.ABSTRACTPhototherapy is universally recognized as the first option for treating neonatal jaundice due to its unparalleled efficiency and safety in reducing the high serum free bilirubin levels and limiting its neurotoxic effects. However, several studies have suggested that phototherapy may elicit a series of short- and long-term adverse reactions associated with pediatric diseases, including hemolysis, allergic diseases, DNA damage or even cancer. The aim of the present review was to summarize the etiology, mechanism, associated risks and thera...
Source: Experimental and Therapeutic Medicine - February 22, 2021 Category: General Medicine Authors: Juan Wang Genxin Guo Aimin Li Wen-Qi Cai Xianwang Wang Source Type: research

4-Methoxybenzylalcohol protects brain microvascular endothelial cells against oxygen-glucose deprivation/reperfusion-induced injury via activation of the PI3K/AKT signaling pathway
Exp Ther Med. 2021 Mar;21(3):252. doi: 10.3892/etm.2021.9684. Epub 2021 Jan 24.ABSTRACTDamage to the blood-brain barrier (BBB) during the process of cerebral ischemic injury is a key factor that affects the treatment of this condition. The present study aimed to assess the potential effects of 4-methoxybenzyl alcohol (4-MA) on brain microvascular endothelial cells (bEnd.3) against oxygen-glucose deprivation/reperfusion (OGD/Rep) using an in vitro model that mimics in vivo ischemia/reperfusion injury. In addition, the present study aimed to explore whether this underlying mechanism was associated with the inhibition of pro-...
Source: Experimental and Therapeutic Medicine - February 22, 2021 Category: General Medicine Authors: Qing Lin Weili Wang Liping Yang Xiaohua Duan Source Type: research

Challenges of phototherapy for neonatal hyperbilirubinemia (Review)
Exp Ther Med. 2021 Mar;21(3):231. doi: 10.3892/etm.2021.9662. Epub 2021 Jan 20.ABSTRACTPhototherapy is universally recognized as the first option for treating neonatal jaundice due to its unparalleled efficiency and safety in reducing the high serum free bilirubin levels and limiting its neurotoxic effects. However, several studies have suggested that phototherapy may elicit a series of short- and long-term adverse reactions associated with pediatric diseases, including hemolysis, allergic diseases, DNA damage or even cancer. The aim of the present review was to summarize the etiology, mechanism, associated risks and thera...
Source: Experimental and Therapeutic Medicine - February 22, 2021 Category: General Medicine Authors: Juan Wang Genxin Guo Aimin Li Wen-Qi Cai Xianwang Wang Source Type: research

4-Methoxybenzylalcohol protects brain microvascular endothelial cells against oxygen-glucose deprivation/reperfusion-induced injury via activation of the PI3K/AKT signaling pathway
Exp Ther Med. 2021 Mar;21(3):252. doi: 10.3892/etm.2021.9684. Epub 2021 Jan 24.ABSTRACTDamage to the blood-brain barrier (BBB) during the process of cerebral ischemic injury is a key factor that affects the treatment of this condition. The present study aimed to assess the potential effects of 4-methoxybenzyl alcohol (4-MA) on brain microvascular endothelial cells (bEnd.3) against oxygen-glucose deprivation/reperfusion (OGD/Rep) using an in vitro model that mimics in vivo ischemia/reperfusion injury. In addition, the present study aimed to explore whether this underlying mechanism was associated with the inhibition of pro-...
Source: Experimental and Therapeutic Medicine - February 22, 2021 Category: General Medicine Authors: Qing Lin Weili Wang Liping Yang Xiaohua Duan Source Type: research

LMTK2 regulates inflammation in lipopolysaccharide-stimulated BV2 cells.
In conclusion, LMTK2 is postulated to regulate neuroinflammation possibly through Nrf2 pathway. The present study is essential to reveal the underlying function of LMTK2 and to identify novel therapeutic targets for drug development in treating neuroinflammation. PMID: 33603828 [PubMed] (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - February 20, 2021 Category: General Medicine Tags: Exp Ther Med Source Type: research

Schizandrin B attenuates hypoxia/reoxygenation injury in H9c2 cells by activating the AMPK/Nrf2 signaling pathway.
Authors: Zhao B, Li GP, Peng JJ, Ren LH, Lei LC, Ye HM, Wang ZY, Zhao S Abstract Schizandrin B exhibits prominent antioxidant and anti-inflammatory effects, and plays an important role in ameliorating myocardial ischemia/reperfusion injury. However, the underlying protective mechanisms remain to be elucidated. The aim of the present study was to explore the cardioprotective effects of schizandrin B against hypoxia/reoxygenation (H/R)-induced H9c2 cell injury, focusing on the role of the adenosine monophosphate-activated protein kinase (AMPK)/nuclear factor erythroid 2-related factor 2 (Nrf2) pathway in this process...
Source: Experimental and Therapeutic Medicine - February 20, 2021 Category: General Medicine Tags: Exp Ther Med Source Type: research

Phenoxythiophene sulfonamide compound B355252 protects neuronal cells against glutamate-induced excitotoxicity by attenuating mitochondrial fission and the nuclear translocation of AIF.
The objective of the present study was to determine whether B355252, a phenoxythiophene sulfonamide derivative, reduces glutamate-induced cell death by inhibiting mitochondrial fission and the nuclear translocation of apoptosis-inducing factor (AIF) in glutamate-challenged HT22 neuronal cells. The results revealed that glutamate treatment led to large increases in the mitochondrial levels of the major fission proteins dynamin-related protein 1 (Drp1) and mitochondrial fission 1 protein (Fis1), but only small elevations in the fusion proteins mitofusin 1 and 2 (Mfn1/2) and optic atrophy 1 (Opa1). In addition, glutamate toxi...
Source: Experimental and Therapeutic Medicine - February 20, 2021 Category: General Medicine Tags: Exp Ther Med Source Type: research

Effects of Streptococcus thermophilus on anterior cruciate ligament transection-induced early osteoarthritis in rats.
Authors: Lin YY, Chen NF, Yang SN, Jean YH, Kuo HM, Chen PC, Feng CW, Liu YW, Lai YC, Wen ZH Abstract Osteoarthritis (OA) is the most common joint disorder and is classically defined as a progressively degenerative disease of articular cartilage. It manifests as joint pain and disability and currently has no comprehensive treatments. The primary purpose of the present study was to test the effects of probiotics, Streptococcus thermophilus (TCI633), on anterior cruciate ligament transection (ACLT)-induced experimental osteoarthritis (OA) in rats. In the current study, the experimental groups were given TCI633 (5x109...
Source: Experimental and Therapeutic Medicine - February 20, 2021 Category: General Medicine Tags: Exp Ther Med Source Type: research

Emerging recognition of the complement system in hepatic ischemia/reperfusion injury, liver regeneration and recovery (Review).
Authors: Hu ZG, Zhou Y, Lin CJ, Yuan GD, He SQ Abstract Hepatic ischemia/reperfusion injury (IRI) is a result of the ischemic cascade and may occur in the settings of liver trauma, resection and transplantation. Components of the complement system have been indicated to be mediators of hepatic IRI and regulators of liver regeneration. As such, their potential to mediate both beneficial and harmful effects render them key targets for therapy. In the present study, the mechanisms of complement mediating hepatic IRI were discussed with a focus on the different functions of complement in hepatic injury and liver recove...
Source: Experimental and Therapeutic Medicine - February 20, 2021 Category: General Medicine Tags: Exp Ther Med Source Type: research

Inhibition of the mevalonate pathway improves myocardial fibrosis.
Authors: Xu H, Shen Y, Liang C, Wang H, Huang J, Xue P, Luo M Abstract The mevalonate (MVA) pathway serves an important role in ventricular remodeling. Targeting the MVA pathway has protective effects against myocardial fibrosis. The present study aimed to investigate the mechanism behind these effects. Primary cultured cardiac fibroblasts from C57BL/6 mice were treated in vitro in 5 groups: i) negative control; ii) angiotensin II (Ang II) model (1x10-5 mol/l); iii) Ang II + rosuvastatin (ROS); iv) Ang II + alendronate (ALE); and v) Ang II + fasudil (FAS). Collagen and crystal violet staining were used to assess mo...
Source: Experimental and Therapeutic Medicine - February 20, 2021 Category: General Medicine Tags: Exp Ther Med Source Type: research

Quantitative T2 mapping of rats with chronic hepatitis.
Authors: Wang X, Hu Y, Lu X, Cai Y, Shu J Abstract The aim of the study was to explore the diagnostic value of T2 mapping in an experimental rat model of chronic liver disease. Chronic hepatitis was induced in Sprague-Dawley male rats (n=88) by intraperitoneal and abdominal subcutaneous injection of carbon tetrachloride in olive oil. The normal control rats (n=12) were similarly injected with the same dose of normal saline. All rats were randomly selected and subjected to T2-weighted/spectral adiabatic inversion recovery and multiple gradient- and spin-echo sequence. After scanning, rats were sacrificed immediately...
Source: Experimental and Therapeutic Medicine - February 20, 2021 Category: General Medicine Tags: Exp Ther Med Source Type: research

Novel applications of platelet concentrates in tissue regeneration (Review).
Authors: Ding ZY, Tan Y, Peng Q, Zuo J, Li N Abstract Numerous studies have explored the suitability of biocompatible materials in regenerative medicine. Platelet concentrates are derived from centrifuged blood and are named according to their biological characteristics, such as platelet-rich plasma, platelet-rich fibrin and concentrated growth factor. Platelet concentrates have gained considerable attention in soft and hard tissue engineering. Indeed, multiple components of autologous platelet concentrates, such as growth factors, fibrin matrix and platelets, serve essential roles in wound healing. Current studies...
Source: Experimental and Therapeutic Medicine - February 20, 2021 Category: General Medicine Tags: Exp Ther Med Source Type: research

Recombinant interleukin-2 stimulates lymphocyte recovery in patients with severe COVID-19.
Authors: Zhu ME, Wang Q, Zhou S, Wang B, Ke L, He P Abstract A recently identified type of pneumonia, referred to as coronavirus disease 2019 (COVID-19), which is caused by severe acute respiratory syndrome coronavirus-2, has rapidly spread worldwide. Lymphopenia and a proinflammatory cytokine storm frequently occur in patients with severe COVID-19. However, to the best of our knowledge, no specific immunomodulatory therapy for COVID-19 has been reported to date. In the present retrospective case-control study, the potential therapeutic effect of recombinant human interleukin-2 (rIL-2) in patients with severe COVID...
Source: Experimental and Therapeutic Medicine - February 20, 2021 Category: General Medicine Tags: Exp Ther Med Source Type: research

Effects of melatonin on protecting against lung injury (Review).
Authors: Wang W, Gao J Abstract Melatonin (MT; N-acetyl-5-methoxy-tryptamine), which has multiple effects and roles, is secreted from the pineal gland at night according to the daily rhythm. In addition to circadian regulation, MT has anti-inflammatory, antioxidant and anticancer functions. Recent studies postulated that MT serves a critical role in apoptosis, anti-ischemic reperfusion injury and anti-proliferative effects on various cells. The current review reported on the underlying mechanism behind the protective effect of MT on lung diseases, such as acute lung injury, acute respiratory distress syndrome, chro...
Source: Experimental and Therapeutic Medicine - February 20, 2021 Category: General Medicine Tags: Exp Ther Med Source Type: research

MicroRNA-489-3p plays a significant role in congenital hypothyroidism through regulating neuronal cell apoptosis via targeting translationally controlled tumor protein 1.
Authors: Liu Q, Li Y, Zhou Y Abstract Accumulating reports have indicated that congenital hypothyroidism (CH) is an endocrine disorder caused by underdeveloped thyroid gland or thyroid dyshormonogenesis. It has been also reported that certain microRNAs (miRNAs) may exert protective effects against the development of CH. However, whether miR-489-3p regulates CH progression remains unclear. The aim of the present study was to investigate the effects of miR-489-3p on CH and elucidate the underlying mechanisms. Therefore, Sprague Dawley rats were injected with propylthiouracil (50 mg/day) to establish a CH model. Rever...
Source: Experimental and Therapeutic Medicine - February 20, 2021 Category: General Medicine Tags: Exp Ther Med Source Type: research

MicroRNA-1323 serves as a biomarker in gestational diabetes mellitus and aggravates high glucose-induced inhibition of trophoblast cell viability by suppressing TP53INP1.
Authors: Liu L, Zhang J, Liu Y Abstract Gestational diabetes mellitus (GDM) leads to poor pregnancy outcomes, and microRNAs (miRNAs/miRs) have been suggested to be associated with GDM, but the pathological mechanisms remain unclear. The present study aimed to investigate the diagnostic value of miR-1323 in GDM patients and its effects on trophoblast cell viability. Additionally, the present study investigated the correlation between miR-1323 and TP53INP1 to understand the pathological mechanism of GDM progression. Reverse transcription-quantitative polymerase chain reaction was used to detect the miR-1323 expressio...
Source: Experimental and Therapeutic Medicine - February 20, 2021 Category: General Medicine Tags: Exp Ther Med Source Type: research

Long non-coding RNA MIAT promotes the proliferation and invasion of laryngeal squamous cell carcinoma cells by sponging microRNA-613.
Authors: Song F, Yang Y, Liu J Abstract Accumulating evidence indicates that the long non-coding RNA myocardial infarction associated transcript (lncRNA MIAT) serves an important role in the progression of a number of cancer types. However, the precise molecular mechanism of MIAT in laryngeal squamous cell carcinoma (LSCC) progression remain elusive. The aim of the current study was to assess the effects and to clarify the molecular mechanism of MIAT on the proliferation and invasion of LSCC cells. The expression of MIAT was detected in LSCC tissues and cells using reverse transcription-quantitative PCR. MTT and co...
Source: Experimental and Therapeutic Medicine - February 20, 2021 Category: General Medicine Tags: Exp Ther Med Source Type: research

Pomelo peel oil alleviates cerebral NLRP3 inflammasome activation in a cardiopulmonary resuscitation rat model.
In conclusion, these results demonstrate the ability of PPO to protect the brain against I/R injury in rats after CPR by a mechanism involving inhibition of the inflammation and pyroptosis mediated by NLRP3 inflammasome activation. PMID: 33603841 [PubMed] (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - February 20, 2021 Category: General Medicine Tags: Exp Ther Med Source Type: research

Long non-coding RNA cyclin-dependent kinase inhibitor 2B antisense ribonucleic acid 1 is associated with in-stent restenosis and promotes human carotid artery smooth muscle cell proliferation and migration by sponging miR-143-3p.
Authors: Ma H, Dong A Abstract Carotid angioplasty and stenting (CAS) is an efficient therapeutic approach for carotid stenosis. However, in-stent restenosis (ISR) frequently occurs and seriously affects the therapeutic efficacy of CAS. Certain non-coding (nc)RNAs serve potential roles in ISR development and progression. Thus, the goals of the present study were to investigate novel biomarkers for ISR development and to further uncover the mechanisms underlying the progression of ISR. The expression of long ncRNA cyclin-dependent kinase inhibitor (CDKN)2B-antisense 1 (AS1) and microRNA (miR)-143-3p in patients with...
Source: Experimental and Therapeutic Medicine - February 20, 2021 Category: General Medicine Tags: Exp Ther Med Source Type: research

MicroRNA-598 inhibits the growth of triple negative breast cancer cells by targeting JAG1.
Authors: Han G, Bai X, Jiang H, He Q Abstract Triple-negative breast cancer (TNBC) has an aggressive phenotype and a poor outcome. The discovery that dysregulated microRNAs (miRNAs) play an important role in tumor progression has led to the suggestion that miRNAs (miRs) could be a potential target for the treatment of TNBC. In the present study, it was demonstrated that miR-598 expression was significantly decreased in TNBC tissues and was related to the degree of lymph node metastasis of patients with TNBC. Ectopic expression of miR-598 suppressed viability and colony formation, as well as increased the apoptosis ...
Source: Experimental and Therapeutic Medicine - February 20, 2021 Category: General Medicine Tags: Exp Ther Med Source Type: research

Decreased expression of serum semaphorin 3B is associated with poor prognosis of patients with hepatocellular carcinoma.
Authors: Li GZ, Shen D, Li GH, Wei M, Zheng LJ, Liu ZL, Sun RQ, Zhou SJ, Zhang ZL, Gao YC Abstract Semaphorin 3B (SEMA-3B), which belongs to the semaphorin family, has an important role in cell apoptosis and inhibition of angiogenesis. A previous study by our group revealed that SEMA-3B was downregulated in tumor tissues of patients with hepatocellular carcinoma (HCC) and exerts anti-motility and anti-invasion effects on tumor cells. However, the serum levels of SEMA-3B and their clinical significance have remained elusive; therefore, the aim of the present study was to monitor its expression in HCC and investigate...
Source: Experimental and Therapeutic Medicine - February 20, 2021 Category: General Medicine Tags: Exp Ther Med Source Type: research

Risk factors for respiratory assistance in premature infants.
Authors: Li HX, Gao CJ, Cheng S, Mao ZL, Wang HY Abstract Premature infants are prone to dyspnea after birth due to immature development, and some infants require respiratory assistance. However, the risk factors for respiratory assistance in premature infants are rarely reported. The present study enrolled 3,394 premature infants (665 infants had been provided with respiratory assistance and 2,729 had not used respiratory assistance) to retrospectively analyze the risk factors associated with respiratory aid. The multivariate logistic regression analysis demonstrated that placental abnormality [odds ratio (OR)=1.2...
Source: Experimental and Therapeutic Medicine - February 20, 2021 Category: General Medicine Tags: Exp Ther Med Source Type: research

Sodium hydrosulfide alleviates dexamethasone-induced cell senescence and dysfunction through targeting the miR-22/sirt1 pathway in osteoblastic MC3T3-E1 cells.
Authors: Li P, Mao WW, Zhang S, Zhang L, Chen ZR, Lu ZD Abstract Glucocorticoid-induced osteoporosis is characterized by osteoblastic cell and microarchitecture dysfunction, as well as a loss of bone mass. Cell senescence contributes to the pathological process of osteoporosis and sodium hydrosulfide (NaHS) regulates the potent protective effects through delaying cell senescence. The aim of the present study was to investigate whether senescence could contribute to dexamethasone (Dex)-induced osteoblast impairment and to examine the effect of NaHS on Dex-induced cell senescence and damage. It was found that the lev...
Source: Experimental and Therapeutic Medicine - February 20, 2021 Category: General Medicine Tags: Exp Ther Med Source Type: research

Circular RNA expression profile in human primary multiple intracranial aneurysm.
Authors: Cao H, Chen J, Lai X, Liu T, Qiu P, Que S, Huang Y Abstract Primary multiple intracranial aneurysm (MIA) is a vascular disease that frequently leads to fatal vascular rupture and subarachnoid hemorrhage. However, the epigenetic regulation associated with MIA has remained largely elusive. Circular RNAs (circRNAs) serve important roles in cardiovascular diseases; however, their association with MIA has remained to be investigated. The present study initially aimed to explore novel mechanisms of MIA through examining circRNA expression profiles. Comprehensive circRNA expression profiles were detected by RNA s...
Source: Experimental and Therapeutic Medicine - February 20, 2021 Category: General Medicine Tags: Exp Ther Med Source Type: research

Experimental models in peritoneal dialysis (Review).
Authors: Yang B, Wang M, Tong X, Ankawi G, Sun L, Yang H Abstract Peritoneal dialysis (PD) is one of the most commonly used dialysis methods and plays an important role in maintaining the quality of life of patients with end-stage renal disease. However, long-term PD treatment is associated with adverse effects on the structure and function of peritoneal tissue, which may lead to peritoneal ultrafiltration failure, resulting in dialysis failure and eventually PD withdrawal. In order to prevent the occurrence of these effects, the important issues that need to be tackled are improvement of ultrafiltration, protectio...
Source: Experimental and Therapeutic Medicine - February 20, 2021 Category: General Medicine Tags: Exp Ther Med Source Type: research

Inhibition of heat shock protein 90 alleviates cholestatic liver injury by decreasing IL-1 β and IL-18 expression.
In conclusion, the HSP90 inhibitor 17-DMAG is able to prevent liver injury in rats with biliary obstruction, and this phenomenon is associated with the reduction of IL-1β and IL-18 expression. PMID: 33603849 [PubMed] (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - February 20, 2021 Category: General Medicine Tags: Exp Ther Med Source Type: research

Discovering pathways in benign prostate hyperplasia: A functional genomics pilot study.
Authors: Chen Z, Ge M Abstract Benign prostate hyperplasia (BPH) is one of the well-known urological neoplasms common in males with an increasing number of associated deaths in aging males. It causes uncomfortable urinary symptoms, including urine flow blockage, and may cause bladder, urinary tract or kidney problems. The histopathological and clinical knowledge regarding BPH is limited. In the present study, an in silico approach was applied that uses genome-scale microarray expression data to discover a wide range of protein-protein interactions in addition to focusing on specific genes responsible for BPH to dev...
Source: Experimental and Therapeutic Medicine - February 20, 2021 Category: General Medicine Tags: Exp Ther Med Source Type: research

Ovarian endometriosis, a precursor of ovarian cancer: Histological aspects, gene expression and microRNA alterations (Review).
Authors: Gaia-Oltean AI, Braicu C, Gulei D, Ciortea R, Mihu D, Roman H, Irimie A, Berindan-Neagoe I Abstract Ovarian endometriosis is a frequent chronic gynecological disease with an uncertain evolution regarding its progression or association with ovarian malignant lesions. The present review summarized the histological aspects, gene expression and microRNA (miRNA/miR) alterations associated with ovarian endometriosis and cancer and their possible interaction. The endometriosis-ovarian cancer interaction has been proposed by certain researchers as a single entity. Histological results indicated that endometriosis ...
Source: Experimental and Therapeutic Medicine - February 20, 2021 Category: General Medicine Tags: Exp Ther Med Source Type: research

COVID-19 and its consequences on mental health (Review).
Authors: Tsamakis K, Tsiptsios D, Ouranidis A, Mueller C, Schizas D, Terniotis C, Nikolakakis N, Tyros G, Kympouropoulos S, Lazaris A, Spandidos DA, Smyrnis N, Rizos E Abstract As one year is approaching since the beginning of the Coronavirus disease 2019 (COVID-19) pandemic, it is important to acknowledge the detrimental effect that it is having on mental health at the individual, societal and public health levels. The current review presents the direct and indirect psychological impact of COVID-19 on the general public, as well as on vulnerable groups, including the elderly, the young, healthcare professionals, p...
Source: Experimental and Therapeutic Medicine - February 20, 2021 Category: General Medicine Tags: Exp Ther Med Source Type: research

Improving motor function after chronic stroke by interactive gaming with a redesigned MR-compatible hand training device.
In this study, we examined the utility of a robot-assisted therapy used in combination with a serious game as a rehabilitation and motor assessment tool in patients with chronic stroke. We evaluated 928 game rounds from 386 training sessions of 8 patients who had suffered an ischemic stroke affecting middle cerebral artery territory that incurred at least 6 months prior. Motor function was assessed with clinical motor scales, including the Fugl-Meyer upper extremity (FM UE) scale, Action Research Arm Test, Modified Ashworth scale and the Box and Blocks test. Robotic device output measures (mean force, force-position correl...
Source: Experimental and Therapeutic Medicine - February 20, 2021 Category: General Medicine Tags: Exp Ther Med Source Type: research

Successful treatment of Gaucher disease type 1 by enzyme replacement therapy over a 10-year duration in a Japanese pediatric patient: A case report.
Authors: Oto Y, Inoue T, Nagai S, Tanaka S, Itabashi H, Shiraisihi M, Nitta A, Murakami N, Ida H, Matsubara T Abstract The prevalence of Gaucher disease (GD) in Japan is much lower than that in Western countries; therefore, data on Japanese pediatric patients with GD type 1 are currently limited. The present study reports on the case of a Japanese pediatric patient with GD type 1 who was diagnosed when she presented with hepatosplenomegaly, thrombocytopenia and slight anemia at the age of 2 years. Serology tests revealed high levels of acid phosphatase (ACP) and angiotensin-converting enzyme (ACE). A bone marrow bi...
Source: Experimental and Therapeutic Medicine - February 20, 2021 Category: General Medicine Tags: Exp Ther Med Source Type: research

Prognostic value of using glucosylceramide synthase and cytochrome P450 family 1 subfamily A1 expression levels for patients with triple-negative breast cancer following neoadjuvant chemotherapy.
In conclusion, co-expression of GCS and CYP1A1 was associated with pCR and DFS in TNBC, which may serve a role in the prediction of the prognosis of patients with TNBC following treatment with NACT. PMID: 33603855 [PubMed] (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - February 20, 2021 Category: General Medicine Tags: Exp Ther Med Source Type: research

ER stress preconditioning ameliorates liver damage after hemorrhagic shock and reperfusion.
Authors: Obert DP, Wolpert AK, Grimm NL, Korff S Abstract The mismatch of oxygen supply and demand during hemorrhagic shock disturbs endoplasmic reticulum (ER) homeostasis. The resulting accumulation of unfolded proteins in the ER lumen, which is a condition that is defined as ER stress, triggers the unfolded protein response (UPR). Since the UPR influences the extent of organ damage following hemorrhagic shock/reperfusion (HS/R) and mediates the protective effects of stress preconditioning before ischemia-reperfusion injury, the current study investigated the mechanisms of ER stress preconditioning and its impact ...
Source: Experimental and Therapeutic Medicine - February 20, 2021 Category: General Medicine Tags: Exp Ther Med Source Type: research

High expression of PIG11 correlates with poor prognosis in gastric cancer.
Authors: Gu J, Zhang S, He X, Chen S, Wang Y Abstract P53-induced gene 11 (PIG11) is an early transcription-related target of p53 that is involved in cell apoptosis and tumor development. However, its biological function in gastric cancer (GC) tissues and relationship with the prognosis of patients with GC have remained elusive. In the present retrospective study, 60 fresh and 790 paraffin-embedded samples of GC were obtained from the Affiliated Hospital of Nantong University (Nantong, China) with complete clinical data from all patients. Reverse transcription-quantitative PCR and tissue microarray-immunohistochemi...
Source: Experimental and Therapeutic Medicine - February 20, 2021 Category: General Medicine Tags: Exp Ther Med Source Type: research

Correlations of cardiac function with inflammation, oxidative stress and anemia in patients with uremia.
Authors: Zhang H, Fan L, Liao H, Tu L, Zhang J, Xu D, Feng J Abstract The present study aimed to analyze the associations of cardiac function with inflammatory cytokines, oxidative stress and anemia in patients with uremia. A total of 79 patients with uremia were selected, and among those, 43 cases were complicated with cardiac dysfunction (observation group) and 36 patients were not (control group). The levels of inflammatory cytokines [C-reactive protein (CRP), interleukin-6 (IL-6) and IL-10], oxidative stress indicators [malondialdehyde (MDA), oxidized low-density lipoprotein (OX-LDL) and advanced oxidation prot...
Source: Experimental and Therapeutic Medicine - February 20, 2021 Category: General Medicine Tags: Exp Ther Med Source Type: research

Preventive and therapeutic significance of octreotide combined with lansoprazole on post-ERCP pancreatitis and its effect on serum amylase, inflammatory factors and immune function.
Authors: Chen Z, Fu H, Fang J, Yang J, Zhu X, Cheng B, Ding T Abstract The study aimed to investigate the preventive and therapeutic significance of octreotide combined with lansoprazole on post-endoscopic retrograde cholangiopancreatography (ERCP) pancreatitis (PEP) and its effects on serum amylase (AMS), inflammatory factors and immune function. A total of 132 patients who underwent ERCP in Shaoxing People's Hospital (Shaoxing, China) were enrolled in the study and allocated into two groups: The study group (octreotide plus lansoprazole, 68 cases) and the control group (octreotide alone, 64 cases). The incidence ...
Source: Experimental and Therapeutic Medicine - February 20, 2021 Category: General Medicine Tags: Exp Ther Med Source Type: research

HSP70 expression before and after treatment and its clinical value in patients with acute angle-closure glaucoma.
Authors: Chen H, Tian A, Wu Y, Li R, Han R, Xu X, Cheng S Abstract The present study aimed to explore the clinical role of heat shock protein 70 (HSP70) in patients with acute angle-closure glaucoma (AACG). Seventy-four AACG patients who were admitted to our hospital from April 2017 to April 2019 were enrolled as a study group (SG). A further 70 healthy people undergoing physical examinations during the same period were enrolled as a control group (CG). HSP70 concentration was compared between the two groups, and the clinical value of this protein in AACG was analyzed. HSP70 concentration in SG was significantly lo...
Source: Experimental and Therapeutic Medicine - February 20, 2021 Category: General Medicine Tags: Exp Ther Med Source Type: research

Diagnostic value and prognostic significance of CTRP9 combined with pentraxin-3 in acute coronary syndrome.
Authors: Jiang N, Zhou S, Wang G, Jiang N, Wang H, Zhao F Abstract The present study aimed to explore the diagnostic value and prognostic significance of C1q/tumor necrosis factor-related protein 9 (CTRP9) combined with pentraxin-3 (PTX-3) in acute coronary syndrome (ACS). A total of 137 patients with coronary heart disease and chest pain were included. Among them, seventy-nine patients with ACS were allocated into a study group and fifty-eight patients with non-cardiac chest pain (NCCP) were allocated into a control group. The serum CTRP9, PTX-3 levels were quantified by ELISA, and their correlation with other ACS...
Source: Experimental and Therapeutic Medicine - February 20, 2021 Category: General Medicine Tags: Exp Ther Med Source Type: research

MicroRNA-34b-5p inhibits proliferation, stemness, migration and invasion of retinoblastoma cells via Notch signaling.
Authors: Zhang S, Cui Z Abstract Retinoblastoma (RB) is one of the most common forms of childhood intraocular cancer. While the occurrence of RB is traditionally associated with dysregulation of the RB1 gene, efforts have been made to assess the role of several other pathways that may result in RB. The Notch signaling pathway has been identified as one of the sentinel pathways in retinal development and has been indicated to serve as a tumor suppressor. However, epigenetic modifications of the Notch signaling pathway, and their consequences on tumor establishment and progression, have received little attention. The...
Source: Experimental and Therapeutic Medicine - February 20, 2021 Category: General Medicine Tags: Exp Ther Med Source Type: research

Double-lobectomy in a steatotic liver transplantation rat model.
Authors: Fan L, Fu Z, Xiong Y, Ye S, Wang Y, Peng G, Ye Q Abstract Establishing a steatotic liver transplantation animal model can be a challenging process, which requires complex microsurgical technologies. The present study established a novel rat model of stable steatotic liver transplantation for marginal liver graft research, which notably minimized the number of animals used for the experiment. Briefly, male Sprague-Dawley rats (n=90) were fed with a high-fat diet (HFD; 60%, kJ) or standard chow diet (SCD) for 8 weeks. The liver enzymes and lipid levels were assessed every week, and the degree of steatosis wa...
Source: Experimental and Therapeutic Medicine - February 20, 2021 Category: General Medicine Tags: Exp Ther Med Source Type: research