Effects of hypothermia on inflammatory cytokine expression in rat liver following asphyxial cardiac arrest
In conclusion, hypothermic treatment for 4 h following asphyxial CA in rats inhibited the increase of pro-inflammatory cytokines and stimulated the expression of anti-inflammatory cytokines compared with the normothermic group. The results of the present study suggested that hypothermic treatment after asphyxial CA reduced liver damage via the regulation of inflammation.PMID:33968162 | PMC:PMC8097226 | DOI:10.3892/etm.2021.10058 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - May 10, 2021 Category: General Medicine Authors: Yoonsoo Park Ji Hyeon Ahn Jeong Hwi Cho Hyun-Jin Tae Tae-Kyeong Lee Bora Kim Jae-Chul Lee Joon Ha Park Myoung Cheol Shin Taek Geun Ohk Jun Hwi Cho Moo-Ho Won Source Type: research

Subacute toxicological evaluation of AT-533 and AT-533 gel in Sprague-Dawley rats
Exp Ther Med. 2021 Jun;21(6):632. doi: 10.3892/etm.2021.10064. Epub 2021 Apr 15.ABSTRACTAs a novel heat shock protein 90 inhibitor, AT-533 exhibits various biological activities in vitro, including anti-viral, anti-tumor and anti-inflammatory activities. Moreover, AT-533 gel, a gel dosage form of AT-533, has been suggested to have anti-keratitis and herpes simplex virus type-1 infection-induced effects on the skin lesions of animals. However, the safety evaluation of AT-533 and AT-533 gel has, to the best of our knowledge, not been examined in in vivo toxicological tests. Therefore, these toxicological tests were carried o...
Source: Experimental and Therapeutic Medicine - May 10, 2021 Category: General Medicine Authors: Yanting Wu Xiaowei Song Shurong Qin Pengxiao Chen Lianzhou Huang Qiaoli Wang Tianhao Shan Feng Liang Xiaofeng Liao Qiuying Liu Yunsheng Huang Yifei Wang Source Type: research

Differential proteomic analysis of tibial subchondral bone from male and female guinea pigs with spontaneous osteoarthritis
Exp Ther Med. 2021 Jun;21(6):633. doi: 10.3892/etm.2021.10065. Epub 2021 Apr 15.ABSTRACTA proteomic study on the tibial subchondral bone in guinea pigs with spontaneous osteoarthritis was performed to investigate the molecular alterations that occur in early osteoarthritis. A total of 132 healthy Hartley guinea pigs (aged 1 month; 66 female and 66 male) were randomly divided into 11 groups of six. Changes in articular cartilage and tibial subchondral bone were assessed using macroscopic examinations and micro-computed tomography. iTRAQ-integrated liquid chromatography-tandem mass spectrometry was used to identify different...
Source: Experimental and Therapeutic Medicine - May 10, 2021 Category: General Medicine Authors: Ying Wang Chengai Wu Jianfeng Tao Danhui Zhao Xu Jiang Wei Tian Source Type: research

Circular RNA expression profiles in the plasma of patients with infantile hemangioma determined using microarray analysis
Exp Ther Med. 2021 Jun;21(6):634. doi: 10.3892/etm.2021.10066. Epub 2021 Apr 15.ABSTRACTCircular RNAs (circRNAs) are noncoding RNAs that have important roles in tumor progression. Previous studies have examined the circRNAs involved in infantile hemangioma (IH) tumors. The present study compared the circRNA levels in plasma samples from patients with IH and control individuals. The circRNA expression profiles were determined using microarray in three pairs of plasma samples from patients with proliferative IH and healthy control subjects. Expression of the identified differentially expressed circRNAs was verified using rev...
Source: Experimental and Therapeutic Medicine - May 10, 2021 Category: General Medicine Authors: Zhiyu Li Yimeng Chai Zifu Zhou Xueqing Li Jianhai Bi Ran Huo Source Type: research

Microarray analysis of the time-dependent expression profiles of long non-coding RNAs in the progression of vein graft stenotic disease
Exp Ther Med. 2021 Jun;21(6):635. doi: 10.3892/etm.2021.10067. Epub 2021 Apr 15.ABSTRACTLong non-coding RNAs (lncRNAs) have been reported to be involved in various biological processes, including cell proliferation and apoptosis. However, the expression profiles of lncRNAs in patients with vein graft restenosis remain unknown. In the present study, the time-dependent expression profiles of genes in vein bypass grafting models were examined by microarray analysis. A total of 2,572 lncRNAs and 1,652 mRNAs were identified to be persistently significantly differentially expressed. Gene Ontology and Kyoto Encyclopedia of Genes ...
Source: Experimental and Therapeutic Medicine - May 10, 2021 Category: General Medicine Authors: Jiayu Shen Hongwei Zhang Chen Lu Jun Gu Yu Zhang Jia Hu Source Type: research

Salidroside overcomes dexamethasone resistance in T-acute lymphoblastic leukemia cells
Exp Ther Med. 2021 Jun;21(6):636. doi: 10.3892/etm.2021.10068. Epub 2021 Apr 15.ABSTRACTThe aim of the present study was to analyze whether the use of salidroside (SAL) could overcome dexamethasone (DEX) resistance in T-acute lymphocytic leukemia cells. The human T-ALL DEX-resistant cell line, CEM-C1 and the DEX-sensitive cell line, CEM-C7 were used in the current study. The proliferation inhibition rates in these cells, treated with SAL and DEX alone, and in combination were detected using a Cell Counting Kit-8 assay, while the morphological changes of the cells were observed using an inverted microscope. Reverse transcri...
Source: Experimental and Therapeutic Medicine - May 10, 2021 Category: General Medicine Authors: Ya-Na Niu Yan Zeng Fang-Fang Zhong Si-Li Long Dan-Wei Ren Xiang Qin Wen-Jun Liu Source Type: research

Secreted frizzled-related protein 4 promotes brown adipocyte differentiation
Exp Ther Med. 2021 Jun;21(6):637. doi: 10.3892/etm.2021.10069. Epub 2021 Apr 15.ABSTRACTSecreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of frizzled proteins. In the present report, the effects of SFRP4 on murine brown adipocyte differentiation were evaluated, which exhibited an intrinsic capacity to differentiate with high efficiency. Brown preadipocytes were isolated from the scapular region of brown adipose tissue, which showed that the overexpression of recombinant active SFRP4 protein at three concentrations (1, ...
Source: Experimental and Therapeutic Medicine - May 10, 2021 Category: General Medicine Authors: Hua Guan Huiyuan Zheng Jin Zhang Aoqi Xiang Yongxin Li Huadong Zheng Lixian Xu Enqi Liu Qi Yu Source Type: research

Gallic acid suppresses colon cancer proliferation by inhibiting SRC and EGFR phosphorylation
In conclusion, GA was indicated to suppress colon cancer proliferation by inhibiting SRC and EGFR phosphorylation.PMID:33968169 | PMC:PMC8097205 | DOI:10.3892/etm.2021.10070 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - May 10, 2021 Category: General Medicine Authors: Xiaoming Lin Guangfei Wang Ping Liu Lei Han Tong Wang Kaili Chen Yonglin Gao Source Type: research

Effects of leukocyte- and platelet-rich plasma on tendon disorders based on < em > in vitro < /em > and < em > in vivo < /em > studies (Review)
Exp Ther Med. 2021 Jun;21(6):639. doi: 10.3892/etm.2021.10071. Epub 2021 Apr 16.ABSTRACTTendon-related disorders are common musculoskeletal system disorders in clinical practice, accounting for 30-50% of all sports-related injuries, and they are difficult to treat due to the hypovascular structure of the tendons. Platelet-rich plasma (PRP), including pure PRP and leukocyte- and platelet-rich plasma (L-PRP), has been attracting increasing attention, as it may stimulate tissue regeneration through the release of growth factors and cytokines. The aim of the present review was to provide a summary of the effects of L-PRP on te...
Source: Experimental and Therapeutic Medicine - May 10, 2021 Category: General Medicine Authors: Xueli Liu Rong Zhang Bin Zhu Yujie Li Xinyue Liu Sheng Guo Chenglong Wang Dingxuan Wang Sen Li Source Type: research

Interleukin-1 induces receptor activator of nuclear factor- κB ligand-independent osteoclast differentiation in RAW264.7 cells
Exp Ther Med. 2021 Jun;21(6):640. doi: 10.3892/etm.2021.10072. Epub 2021 Apr 16.ABSTRACTInterleukin-1 (IL-1) is a pro-inflammatory cytokine which induces bone destruction in various diseases, such as osteoporosis and rheumatoid arthritis. RAW264.7 cells are frequently used in studies as osteoclast precursors, however it remains unclear whether IL-1 can induce osteoclast differentiation from RAW264.7 cells without the stimulation of receptor activator of nuclear factor-κB ligand (RANKL). Hence, the present study aimed to investigate the effects of IL-1 on the formation of osteoclasts from RAW264.7 cells. The cell viab...
Source: Experimental and Therapeutic Medicine - May 10, 2021 Category: General Medicine Authors: Rongdong Liao Zhuoxi Feng Wei Li Rubing Liu Xinrou Xu Shun Yao Jing Tian Source Type: research

Clinical and diagnostic value of the combination of lymphocyte count and creatine kinase in the detection of coronavirus 2019
Exp Ther Med. 2021 Jun;21(6):641. doi: 10.3892/etm.2021.10073. Epub 2021 Apr 16.ABSTRACTThe present study aimed to investigate the diagnostic efficiency of the absolute number of lymphocytes (LYM) and creatine kinase (CK) levels in the diagnosis of coronavirus disease 2019 (COVID-19). For this, the clinical data from 84 patients with COVID-19 admitted to Tianjin Haihe Hospital (Tianjin, China) between January and February 2020 were collected. The patients were divided into the following groups: The common COVID-19 group (n=61) and severe COVID-19 group (n=23). In addition, 30 healthy subjects were included as a control gro...
Source: Experimental and Therapeutic Medicine - May 10, 2021 Category: General Medicine Authors: Yanqing Du Xiang Wang Zhonghua Qin Lixia Zhang Source Type: research

Life-threatening complications of hyperemesis gravidarum
Exp Ther Med. 2021 Jun;21(6):642. doi: 10.3892/etm.2021.10074. Epub 2021 Apr 16.ABSTRACTHyperemesis gravidarum (HG) refers to severe nausea and emesis noted during pregnancy. However, no consensus exists on the specific diagnostic criteria that can be used for this condition. The aim of the present systematic review was to summarize the available evidence regarding the severe complications observed during HG with a heightened risk of fatality. A systematic search was conducted on PubMed, Cochrane Library, EMBASE and WILEY databases for the relevant publications regarding the severe and life-threatening complications of HG....
Source: Experimental and Therapeutic Medicine - May 10, 2021 Category: General Medicine Authors: Stefan L Popa Maria Barsan Alexandra Caziuc Cristina Pop Lucian Muresan Luminita Celia Popa Lacramioara Perju-Dumbrava Source Type: research

Diagnostic accuracy of ultrasonography for the prenatal diagnosis of esophageal atresia and tracheoesophageal fistula
Exp Ther Med. 2021 Jun;21(6):643. doi: 10.3892/etm.2021.10075. Epub 2021 Apr 18.ABSTRACTUltrasound is recommended as a first-line requirement prior to MRI or amniotic fluid analysis, which have high diagnostic accuracy for esophageal atresia (EA). Therefore, the aim of the present prospective study was to evaluate the accuracy of high-performance ultrasound for the prenatal examination of EA/tracheoesophageal fistula (TOF). In total, 64 pregnant women with fetuses suspected of having EA/TOF participated in the study. The gestational age of the fetuses ranged between 16 and 40 weeks, with a mean of 26.33±3.57 weeks. ...
Source: Experimental and Therapeutic Medicine - May 10, 2021 Category: General Medicine Authors: Cong Wang Xiaofei Ning Yangcan Duan Zhonglu Zhang Shaochun Wang Source Type: research

Upregulation of long non-coding RNA MYU promotes proliferation, migration and invasion of esophageal squamous cell carcinoma cells
Exp Ther Med. 2021 Jun;21(6):644. doi: 10.3892/etm.2021.10076. Epub 2021 Apr 18.ABSTRACTEsophageal squamous cell carcinoma (ESCC) is a common malignant tumour type of the digestive system. Long non-coding RNA (lncRNA) c-Myc upregulated (MYU), also known as VPS9 domain-containing 1 antisense 1, was recently discovered. However, the expression of lncRNA MYU in ESCC and its role in tumour progression have remained elusive. In the present study, the expression of lncRNA MYU, Ki-67 and the epithelial-mesenchymal transition-related proteins E-cadherin and Vimentin in ESCC tissues was detected by reverse transcription-quantitativ...
Source: Experimental and Therapeutic Medicine - May 10, 2021 Category: General Medicine Authors: Shudong Gu Li Qian Yan Liu Jiefei Miao Hong Shen Shu Zhang Guoxin Mao Source Type: research

lncRNA NORAD promotes the progression of osteosarcoma via targeting of miR-155-5p
Exp Ther Med. 2021 Jun;21(6):645. doi: 10.3892/etm.2021.10077. Epub 2021 Apr 18.ABSTRACTOsteosarcoma (OS) is the most common malignant bone tumor in teens. Non-coding RNA activated by DNA damage (NORAD), a long non-coding RNA (lncRNA), has been reported to be involved in cancer biology, although its role in OS remains largely unknown. In the present study reverse transcription-quantitative PCR (RT-qPCR) was used to determine the expression levels of NORAD and miR-155-5p in samples from patients with OS. OS cell lines (Saos-2 and U2OS) were used as cell models. The biological influence of NORAD on OS cells was studied in vi...
Source: Experimental and Therapeutic Medicine - May 10, 2021 Category: General Medicine Authors: Ye Wang Bin Zhou Liping Yan Jianhui Wu Zhijie Xing Shaochun Zhang Fusheng Xiang Source Type: research

Hypoxia-inducible factor-1 α regulates PI3K/AKT signaling through microRNA-32-5p/PTEN and affects nucleus pulposus cell proliferation and apoptosis
Exp Ther Med. 2021 Jun;21(6):646. doi: 10.3892/etm.2021.10078. Epub 2021 Apr 18.ABSTRACTIntervertebral disc degeneration and resulting low back pain arises from the programmed apoptosis of nucleus pulposus cells (NPCs). Recent studies show that hypoxia-inducible factor-1α plays a vital role in the etiology and pathogenesis of disc degeneration. However, the underlying mechanism of HIF-1α in NPCs is unclear. The present study identified 994 significant differentially expressed miRNAs by analyzing microarray data downloaded from the Gene Expression Omnibus database. MicroRNA(miR)-32-5p expression was 2.81-fold up...
Source: Experimental and Therapeutic Medicine - May 10, 2021 Category: General Medicine Authors: Daolu Zhan Mingxia Lin Jian Chen Wentao Cai Jian Liu Yehan Fang Yibo Li Bin Wu Guangji Wang Source Type: research

Sinomenine activation of Nrf2 signaling prevents inflammation and cerebral injury in a mouse model of ischemic stroke
In conclusion, the findings of the present study identified a novel pathway through which SINO may exert its anti-inflammatory and cerebral protective functions, and provided a molecular basis for the potential applications of SINO in the treatment of cerebral inflammatory disorders.PMID:33968178 | PMC:PMC8097210 | DOI:10.3892/etm.2021.10079 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - May 10, 2021 Category: General Medicine Authors: Fangfang Bi Yiyong Zhang Wenbo Liu Keliang Xie Source Type: research

Efficiency of antiviral treatment in COVID-19
Exp Ther Med. 2021 Jun;21(6):648. doi: 10.3892/etm.2021.10080. Epub 2021 Apr 19.ABSTRACTThe severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic is responsible for generating a global effort to discover urgent therapeutic solutions to limit the human damage caused by COVID-19. In the period of April to June 2020, 105 patients diagnosed with COVID-19 met the conditions for inclusion in the present study. They were treated with antiviral therapy according to local guidelines: D group (53 cases), treated with darunavir/ritonavir (DRV/r); and K group (52 cases), treated with lopinavir/ritonavir (LPV/r). Patien...
Source: Experimental and Therapeutic Medicine - May 10, 2021 Category: General Medicine Authors: Nicoleta Negrut Adriana Codrean Ioana Hodisan Simona Bungau Delia Mirela Tit Ruxandra Marin Tapan Behl Florin Banica Camelia C Diaconu Delia Carmen Nistor-Cseppento Source Type: research

Changes in peri-ocular anatomy and physiology in pseudoexfoliation syndrome (Review)
Exp Ther Med. 2021 Jun;21(6):650. doi: 10.3892/etm.2021.10082. Epub 2021 Apr 19.ABSTRACTPseudoexfoliation syndrome (PEX) is characterized by the deposition of proteinaceous material in the anterior ocular segment (resulting in ophthalmic pathologies such as glaucoma and increased risk of complications in cataract surgery), but also by several systemic manifestations. The involvement of peri-ocular tissues in PEX, including the eyelid skin, lacrimal gland, conjunctiva, orbital fat and vessels, as well as the optic nerve, has been reported by several previous studies. The peri-ocular effects of PEX include the development of...
Source: Experimental and Therapeutic Medicine - May 10, 2021 Category: General Medicine Authors: Efstathios T Detorakis Georgios Bontzos Eleni E Drakonaki Demetrios A Spandidos Source Type: research

Protein kinase N1 promotes proliferation and invasion of liver cancer
Exp Ther Med. 2021 Jun;21(6):651. doi: 10.3892/etm.2021.10083. Epub 2021 Apr 19.ABSTRACTProtein kinase (PK) N1, also called PKC-related protein 1, participates in the proliferation, invasion and metastasis of various malignant tumors. However, the role of PKN1 in liver cancer remains to be elucidated. The present study investigated the expression of PKN1 using immunohistochemistry in surgical specimens from 36 patients and analyzed the correlation with VEGF, microvascular density (MVD), cell proliferation index (Ki67) and clinicopathological parameters. PKN1 was highly expressed in hepatocellular carcinoma (HCC) and was po...
Source: Experimental and Therapeutic Medicine - May 10, 2021 Category: General Medicine Authors: Xia Wang Yansong Ge Mingqi Shi Hanhan Dai Wei Liu Peiyuan Wang Source Type: research

miR-221-3p and miR-222-3p regulate the SOCS3/STAT3 signaling pathway to downregulate the expression of NIS and reduce radiosensitivity in thyroid cancer
In conclusion, the findings of the present study suggested that miR-221-3p and miR-222-3p may downregulate the expression levels of NIS and promote radioresistance. The potential mechanism was hypothesized to be associated with the miR-221-3p and miR-222-3p targeting of the SOCS3 gene, which may subsequently activate the STAT3 signaling pathway.PMID:33968182 | PMC:PMC8097237 | DOI:10.3892/etm.2021.10084 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - May 10, 2021 Category: General Medicine Authors: Ting Ye Lili Zhong Xuemei Ye Jie Liu Linfa Li Heqing Yi Source Type: research

Anticancer activity of bergenin against cervical cancer cells involves apoptosis, cell cycle arrest, inhibition of cell migration and the STAT3 signalling pathway
Exp Ther Med. 2021 Jun;21(6):653. doi: 10.3892/etm.2021.10085. Epub 2021 Apr 19.ABSTRACT[This retracts the article DOI: 10.3892/etm.2019.7380.].PMID:33968183 | PMC:PMC8097232 | DOI:10.3892/etm.2021.10085 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - May 10, 2021 Category: General Medicine Authors: Xingyuan Shi Manman Xu Kai Luo Wenjin Huang Hongwei Yu Tongchong Zhou Source Type: research

Apatinib inhibits gastric carcinoma development by regulating the expression levels of IL-17 via the Bax/Bcl-2 signaling pathway
Exp Ther Med. 2021 Jun;21(6):654. doi: 10.3892/etm.2021.10086. Epub 2021 Apr 19.ABSTRACTGastric carcinoma is a common type of gastrointestinal tumor with high morbidity and mortality rates. IL-17 is a newly discovered cytokine that has been reported to serve an important role in the development of gastric carcinoma. The potential effect of apatinib on IL-17 expression levels in the development of gastric carcinoma has been rarely reported. The present study aimed to investigate the potential mechanism of IL-17 and apatinib in the development of gastric carcinoma. A total of 30 tumor and para-carcinoma tissues were collecte...
Source: Experimental and Therapeutic Medicine - May 10, 2021 Category: General Medicine Authors: Tianxi Wang Jun Zhang Lihong Cui Source Type: research

Effect of < em > Porphyromonas gingivalis < /em > lipopolysaccharide on calcification of human umbilical artery smooth muscle cells co-cultured with human periodontal ligament cells
In conclusion, co-culture with HPDLCs increased the effect of Pg-LPS to stimulate the calcification of HUASMCs. It was suggested that besides the inflammation, periodontitis may promote the occurrence of vascular calcification. The study indicated that periodontal treatment of subgingival scaling to reduce and/or control Porphyromonas gingivalis may decrease the occurrence or severity of vascular calcification.PMID:33968185 | PMC:PMC8097230 | DOI:10.3892/etm.2021.10087 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - May 10, 2021 Category: General Medicine Authors: Jing Li Jing Deng Shuxian Shang Guirong Liu Wenbin Song Pei Sun Wenjing Jiang Keqing Pan Source Type: research

Regulatory mechanism of calcium/calmodulin-dependent protein kinase II in the occurrence and development of ventricular arrhythmia (Review)
Exp Ther Med. 2021 Jun;21(6):656. doi: 10.3892/etm.2021.10088. Epub 2021 Apr 20.ABSTRACTVentricular arrhythmia (VA) is a highly fatal arrhythmia that involves multiple ion channels. Of all sudden cardiac death events, ~85% result from VAs, including ventricular tachycardia and ventricular fibrillation. Calcium/calmodulin-dependent pro-tein kinase II (CaMKII) is an important ion channel regulator that participates in the excitation-contraction coupling of the heart, and as such is important for regulating its electrophysiological function. CaMKII can be activated in a Ca2+/calmodulin (CaM)-dependent or Ca2+/CaM-independent ...
Source: Experimental and Therapeutic Medicine - May 10, 2021 Category: General Medicine Authors: Kexin Ma Guoping Ma Zijing Guo Gang Liu Wenjie Liang Source Type: research

Association of obstructive sleep apnea/hypopnea syndrome with glaucomatous optic neuropathy and ocular blood flow
Exp Ther Med. 2021 Jun;21(6):657. doi: 10.3892/etm.2021.10089. Epub 2021 Apr 20.ABSTRACTThe present study aimed to investigate the prevalence of glaucomatous optic neuropathy in patients with obstructive sleep apnea/hypopnea syndrome (OSAHS). In total, 83 subjects (45 cases of severe OSAHS and 38 controls) underwent polysomnographic assessment and were evaluated for the severity of the disease using the Apnea-Hypopnea index. A detailed ophthalmologic exam was then performed, including measurement of the intraocular pressure (IOP) with a Goldmann applanation tonometer and Pascal dynamic contour tonometer (DCT), recording of...
Source: Experimental and Therapeutic Medicine - May 10, 2021 Category: General Medicine Authors: Georgios Dalianis Alexandra Trivli Aliki Limnopoulou Andreas Stavrakis Chryssa Terzidou Efstathios T Detorakis Vassilios Kozobolis Georgios Labiris Source Type: research

Facing coronavirus disease 2019: What do we know so far? (Review)
Exp Ther Med. 2021 Jun;21(6):658. doi: 10.3892/etm.2021.10090. Epub 2021 Apr 20.ABSTRACTAlthough the World Health Organization declared the outbreak of coronavirus disease 2019 (COVID-19), which originated in China, as a public health emergency of international concern as early as January 30, 2020, the current COVID-19 epidemic is spreading rapidly. As of April 19, 2020, total of 2,392,165 confirmed cases had been reported in 211 countries and regions, with 614,421 (25.68%) cured cases and 164,391 (6.87%) deaths. Scientists and clinicians have made great efforts to learn much about COVID-19 so that it can be controlled as ...
Source: Experimental and Therapeutic Medicine - May 10, 2021 Category: General Medicine Authors: Qie Guo Wen Xu Pan-Feng Wang Hong-Yan Ji Xiao-Lei Zhang Kai Wang Jing Li Source Type: research

MicroRNA-219a-2-3p modulates the proliferation of thyroid cancer cells via the HPSE/cyclin D1 pathway
Exp Ther Med. 2021 Jun;21(6):659. doi: 10.3892/etm.2021.10091. Epub 2021 Apr 20.ABSTRACTHeparanase (HPSE) is an endo-β-D-glucuronidase overexpressed in different types of human cancer, and a predicted target of microRNA (miRNA/miR)-219a-2-3p in thyroid cancer. The present study aimed to investigate the potential role of HPSE and miR-219a-2-3p in thyroid cancer, and the molecular mechanism of miR-219a-2-3p regulating the proliferation of thyroid cancer cells via HPSE was confirmed. Immunohistochemistry analysis was performed to detect HPSE expression in thyroid cancer sections. In addition, reverse transcription-quanti...
Source: Experimental and Therapeutic Medicine - May 10, 2021 Category: General Medicine Authors: Chuanjia Yang Siyang Zhang Xiaoying Chang Yonglian Huang Dongxu Cui Zhen Liu Source Type: research

Transcriptomic analysis and competing endogenous RNA network in the human endometrium between proliferative and mid-secretory phases
Exp Ther Med. 2021 Jun;21(6):660. doi: 10.3892/etm.2021.10092. Epub 2021 Apr 20.ABSTRACTSuccessful embryo implantation is the first step for establishing natural pregnancy and is dependent on the crosstalk between the embryo and a receptive endometrium. However, the molecular signaling events for successful embryo implantation are not entirely understood. To identify differentially expressed transcripts [long-noncoding RNAs (lncRNAs), microRNAs (miRNAs) and mRNAs] and competing endogenous RNA (ceRNA) networks associated with endometrial receptivity, the current study analyzed gene expression profiles between proliferative ...
Source: Experimental and Therapeutic Medicine - May 10, 2021 Category: General Medicine Authors: Seong-Lan Yu Tae-Hyun Kim Young-Hyun Han Yujin Kang Da-Un Jeong Dong Chul Lee Jaeku Kang Seok-Rae Park Source Type: research

Apoptosis in HUVECs induced by microRNA-616-3p via X-linked inhibitor of apoptosis protein targeting
Exp Ther Med. 2021 Jun;21(6):661. doi: 10.3892/etm.2021.10093. Epub 2021 Apr 21.ABSTRACTAtherosclerosis causes stroke and coronary heart disease and is associated with a high mortality rate worldwide. However, the pathogenesis of atherosclerosis remains unclear. Endothelial cell apoptosis is one of the early changes observed in atherosclerosis. Previous studies have found that microRNA (miR)-616-3p may be involved in the development of atherosclerosis, but the specific mechanism is not clear. The present study aimed to investigate whether miR-616-3p is involved in endothelial cell apoptosis and its underlying mechanism. Th...
Source: Experimental and Therapeutic Medicine - May 10, 2021 Category: General Medicine Authors: Hua Chen Xi Liu Yun Wu Xiayin Wu Xiaoli Wen Yanan Lu Xingsheng Zhao Source Type: research

Influence of fine particulate matter and its pure particulate fractions on pulmonary immune cells and cytokines in mice
Exp Ther Med. 2021 Jun;21(6):662. doi: 10.3892/etm.2021.10094. Epub 2021 Apr 21.ABSTRACTParticulate matter with a diameter ≤2.5 µm (PM2.5) has a complex composition and has been associated with the incidence of cardiopulmonary disease and premature death in humans. However, whether pure particulate fractions of PM2.5 (PPP2.5), which are composed primarily of carbon, are responsible for the toxicity caused by ambient particulate matter (original PM2.5 particles, OPP2.5) is currently unclear. The present study assessed the acute toxic effects of OPP2.5 sampled in Beijing, China and of its PPP2.5 fraction in male BAL...
Source: Experimental and Therapeutic Medicine - May 10, 2021 Category: General Medicine Authors: Zhouguang Jiao Zhanbo Wen Wenhui Yang Lingfei Hu Jinsong Li Source Type: research

Prevalence of < em > GJB2 < /em > gene mutations correlated to presence of clinical and environmental risk factors in the etiology of congenital sensorineural hearing loss of the Romanian population
Exp Ther Med. 2021 Jun;21(6):612. doi: 10.3892/etm.2021.10044. Epub 2021 Apr 14.ABSTRACTAlthough etiologically heterogeneous at least 50% of all early on-set hearing losses have a genetic cause and of these, the large majority, 75-80% are most probably autosomal recessive and 70% are non-syndromic. The rest of the congenital hearing losses are determined by clinical and environmental factors such as ototoxic medication, prematurity, and complications at birth. During the last decade it became clear that 50-80% of all such afflictions result from mutations in a single gene, GJB2, which encodes the protein Connexin 26. In or...
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Alexandra Neagu Adela-Ioana Mocanu Alexandru Bonciu Gabriella Coad ă Horia Mocanu Source Type: research

PHLDA3 inhibition attenuates endoplasmic reticulum stress-induced apoptosis in myocardial hypoxia/reoxygenation injury by activating the PI3K/AKT signaling pathway
In conclusion, in the process of H/R injury, PHLDA3 inhibition reduced ERS-induced apoptosis and H/R injury by activating the PI3K/AKT pathway. PHLDA3 may be a therapeutic target for the treatment of MIRI.PMID:33936270 | PMC:PMC8082641 | DOI:10.3892/etm.2021.10045 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Kai Liu Ying Chen Fen Ai Yun-Qian Li Kun Zhang Wei-Tong Zhang Source Type: research

Novel ARID1B variant inherited from somatogonadal mosaic mother in siblings with Coffin-Siris syndrome 1
Exp Ther Med. 2021 Jun;21(6):614. doi: 10.3892/etm.2021.10046. Epub 2021 Apr 14.ABSTRACTCoffin-Siris syndrome1 (CSS1; Online Mendelian Inheritance in Man no. 135900) is a multiple malformation syndrome characterized by intellectual and/or developmental delay, and hypoplastic or absent fifth fingernails and/or toenails. AT-rich interaction domain-containing protein 1B (ARID1B) is the most frequently mutated gene in CSS1 and the majority of reported cases have been sporadic. Using whole-exome sequencing, the present study identified two siblings with CSS1 with a novel heterozygous co-segregating pathogenic variant in the ARI...
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Zhong Min Cheng Qian Dai Ying Source Type: research

Exogenous transforming growth factor- β1 prevents the inflow of fluoride to ameleoblasts through regulation of voltage-gated chloride channels 5 and 7
Exp Ther Med. 2021 Jun;21(6):615. doi: 10.3892/etm.2021.10047. Epub 2021 Apr 14.ABSTRACTDental fluorosis is a global issue. Although there are multiple causes of dental fluorosis, the precise mechanism remains controversial. Previous studies have demonstrated that extracellular fluoride may promote an accumulation of fluoride ions in ameloblasts, which may induce oxidative and endoplasmic reticulum stresses, leading to dental fluorosis. However, the exact process by which fluoride ions enter cells has not been determined. In the present study, intracellular fluoride concentration was determined using a newly developed spec...
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Mei Ji Xuejing Duan Xiaohui Han Jing Sun Dongsheng Zhang Source Type: research

ΜicroRNA-122 protects against ischemic stroke by targeting Maf1
In conclusion, the results suggested that miR-122 improved the outcome of acute ischemic stroke by reducing the expression of Maf1.PMID:33936273 | PMC:PMC8082594 | DOI:10.3892/etm.2021.10048 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Mengmeng Wang Xiaoman Liu Yu Wu Yi Wang Jiahui Cui Jing Sun Ying Bai Ming-Fei Lang Source Type: research

Changes in bone mineral density, 25-hydroxyvitamin D < sup > 3 < /sup > and inflammatory factors in patients with hyperthyroidism
Exp Ther Med. 2021 Jun;21(6):617. doi: 10.3892/etm.2021.10049. Epub 2021 Apr 14.ABSTRACTThe present study aimed to evaluate changes in bone mineral density, 25-hydroxyvitamin D3 [25-(OH)D3] and inflammatory factors in patients with hyperthyroidism, in order to determine the correlations with the pathogenesis of hyperthyroidism. A total of 55 patients with hyperthyroidism (observation group) and 53 healthy patients (control group) enrolled at Weifang People's Hospital from March 2017 to February 2018 were randomly enrolled. The thyroid function, bone mineral density, 25-(OH)D3 and inflammatory factors were measured and comp...
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Yali Zhou Xixia Wang Maoyuan Xin Haiting Zhuang Source Type: research

Total flavonoids of < em > Rhizoma Drynariae < /em > combined with calcium attenuate osteoporosis by reducing reactive oxygen species generation
Exp Ther Med. 2021 Jun;21(6):618. doi: 10.3892/etm.2021.10050. Epub 2021 Apr 14.ABSTRACTIn the present study, the effects of total flavonoids of Rhizoma Drynariae (TFRD) and calcium carbonate (CaCO3) on osteoporosis (OP) were assessed in a rat model of OP. For this purpose, 36 Sprague-Dawley rats, aged 3 months, were randomly divided into a group undergoing sham surgery (sham-operated group), model group (OP group), CaCO3 group (OP + CaCO3 group), TFRD group (OP + TFRD group), TFRD combined with CaCO3 group (OP + TFRD + CaCO3 group) and TFRD and CaCO3 combined with N-acetyl cysteine group (OP + TFRD + CaCO3 + NAC group). T...
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Panyun Mu Yimei Hu Xu Ma Jingru Shi Zhendong Zhong Lingyuan Huang Source Type: research

Polycomb group protein Bmi1 is required for the neuronal differentiation of mouse induced pluripotent stem cells
Exp Ther Med. 2021 Jun;21(6):619. doi: 10.3892/etm.2021.10051. Epub 2021 Apr 14.ABSTRACTInduced pluripotent stem cells (iPSCs) reprogrammed by somatic cells may be used as a potentially novel treatment regimen in stem cell regenerative medicine, particularly in the central nervous system (CNS). In the present study, iPSCs were generated using mouse embryonic fibroblasts by ectopic overexpression of Sox-2, Oct-3/4, Klf-4 and c-Myc, and cultured under the same conditions as that used for embryonic stem cells. The neuronal differentiation capacity of mouse iPSCs was examined, and the involvement of the formation of embryoid ...
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Wei Shan Liping Zhou Lizhen Liu Deju Lin Qin Yu Source Type: research

Transforming growth factor- β1-induced podocyte injury is associated with increased microRNA-155 expression, enhanced inflammatory responses and MAPK pathway activation
Exp Ther Med. 2021 Jun;21(6):620. doi: 10.3892/etm.2021.10052. Epub 2021 Apr 14.ABSTRACTMicroRNA-155 (miR-155) is associated with various diseases. However, the potential role of miR-155 in early glomerular disease (EGD) remains elusive. In the present study, the clinical significance of urinary miR-155 expression was explored in patients with EGD using receiver operating characteristic curve analysis. Conditionally immortalized mouse podocytes were cultured in vitro and treated with transforming growth factor-β1 (TGF-β1) at different concentrations and durations. The gene expression levels of mRNAs and miR-155 w...
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Xintong Zheng Qiuhong Zhong Xu Lin Xianjun Gu Xiaoyan Ling Zhao Liang Qing Qin Xiuri Du Source Type: research

Joint line plate fixation for tibial plateau fractures caused by hyperextension varus
In conclusion, internal fixation with a joint line plate is an appropriate treatment for tibial plateau fractures involving the anteromedial margin with good clinical efficacy.PMID:33936278 | PMC:PMC8082576 | DOI:10.3892/etm.2021.10053 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Yu-Cheng Huang Jing Jiao Wen-Jun Cheng Fei Xiao Wei Zuo Jun-Wen Wang Source Type: research

miR-491-5p inhibits the proliferation and migration of A549 cells by FOXP4
Exp Ther Med. 2021 Jun;21(6):622. doi: 10.3892/etm.2021.10054. Epub 2021 Apr 14.ABSTRACTAberrant expression of microRNAs (miRNAs/miRs) plays a key role in the development of non-small cell lung cancer (NSCLC). In the present study, lower miRNA (miR)-491-5p levels and a higher forkhead box P4 (FOXP4) mRNA level were observed in NSCLC tissues and cell lines, compared to adjacent tissues and the normal human lung epithelial cell line BEAS-2B, respectively. A549 cell proliferation and migration were inhibited upon transfection of miR-491-5p mimics compared to miR-negative control (NC) mimics. In addition, compared to miR-NC mi...
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Fuyong Wu Aiping Ji Zhenkun Zhang Jinfang Li Penglong Li Source Type: research

Effect of media distraction (audio-visual and music) for pain and anxiety control in patients undergoing shock-wave lithotripsy: A systematic review and meta-analysis
Exp Ther Med. 2021 Jun;21(6):623. doi: 10.3892/etm.2021.10055. Epub 2021 Apr 14.ABSTRACTAudio-visual (AV) or music distraction may be used to reduce pain during several healthcare procedures. The present manuscript is a systematic review and meta-analysis to assess the effectiveness of media distraction in reducing pain and anxiety in extracorporeal shock wave lithotripsy (ESWL) patients. The PubMed, Embase, Scopus, BioMed Central, Ovoid and CENTRAL (Cochrane Central Register of Controlled Trials) databases were screened for studies assessing the role of media distraction (music/AV media) in reducing pain and anxiety of ES...
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Weihua Hu Ke Yang Li Zhang Xu Lu Source Type: research

Chemical hemorrhagic cystitis: Diagnostic and therapeutic pitfalls (Review)
In conclusion, CC is a disease with an increasing incidence, challenging to diagnose, which is frequently mistreated, and has multiple treatment modalities that still require standardization in administration and sequencing.PMID:33936281 | PMC:PMC8082586 | DOI:10.3892/etm.2021.10056 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Razvan-Cosmin Petca Razvan-Ionut Popescu Cristian Toma Mihai Cristian Dumitrascu Aida Petca Florica Sandru Calin Bogdan Chibelean Source Type: research

NICE-3-knockdown induces cell cycle arrest and autophagy in lung adenocarcinoma cells via the AKT/mTORC1 signaling pathway
Exp Ther Med. 2021 Jun;21(6):625. doi: 10.3892/etm.2021.10057. Epub 2021 Apr 15.ABSTRACTThe NICE-3 protein serves an oncogenic role in hepatocellular carcinoma, but its role in lung adenocarcinoma (LUAD) remains unknown. The aim of the present study was to investigate the potential role and underlying mechanisms of NICE-3 in LUAD. In the present study, NICE-3 expression in LUAD tissues and its association with patient prognosis were analyzed using datasets from The Cancer Genome Atlas and Gene Express Omnibus. After NICE-3-knockdown with small interfering RNA in LUAD cells, cell proliferation was measured by cell counting,...
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Longxia Du Youru Wu Xiaodan Han Chen Wang Aili Li Guojin Huang Source Type: research

Interleukin-12 exacerbates symptoms in an MRL/MpJ-Faslpr mouse model of systemic lupus erythematosus
Exp Ther Med. 2021 Jun;21(6):627. doi: 10.3892/etm.2021.10059. Epub 2021 Apr 15.ABSTRACTInterleukin (IL)-12 modulates the generation and function of a variety of immune cells and serves an important role in the pathogenesis of autoimmune diseases. However, the precise role of IL-12 in the pathogenesis of systemic lupus erythematosus (SLE) remains to be elucidated. In the present study, the serum levels of IL-12 in patients with SLE were determined using an ELISA. The association between serum levels of IL-12 and clinical and laboratory indices, specifically, disease activity and complement 3, were analyzed. Recombinant IL-...
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Ling Li Xiaojun Sun Sisi Wu Xin Yuan Bingxin Liu Xueping Zhou Source Type: research

Simvastatin intervention mitigates hypercholesterolemia-induced alveolar bone resorption in rats
Exp Ther Med. 2021 Jun;21(6):628. doi: 10.3892/etm.2021.10060. Epub 2021 Apr 15.ABSTRACTSimvastatin promotes bone formation and increases bone mineral density in patients with hyperlipidemia and ameliorates hypercholesterolemia-induced microstructure changes in the jaw bone of animals. However, whether and how treatment with simvastatin can modulate the hypercholesterolemia-induced alveolar bone resorption is unclear. The present study aimed to examine the therapeutic efficacy and potential mechanisms of simvastatin application in hypercholesterolemia-induced alveolar bone resorption. The association between hyperlipidemia...
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Xiaoli Gao Jianhua Zhou Yuanyuan Bian Shengyun Huang Dongsheng Zhang Source Type: research

Thioredoxin relieves lipopolysaccharide-induced acute kidney injury in mice by reducing inflammation, oxidative stress and apoptosis
Exp Ther Med. 2021 Jun;21(6):629. doi: 10.3892/etm.2021.10061. Epub 2021 Apr 15.ABSTRACTAcute kidney injury (AKI) is a serious disease with rapid onset and a high mortality rate. It is therefore particularly important to identify a suitable method for treating AKI. Thioredoxin (Trx) is a potent anti-inflammatory and anti-oxidant protein that is prevalent in living organisms. The aim of the present study was to facilitate the clinical treatment of AKI via the study of Trx. Lipopolysaccharide (LPS) was used to construct an AKI model in mice and the mice were pre-treated with Trx to examine its effect on AKI. In addition, hum...
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Jingjing Wang Wenjuan Zhang Guoyuan Lu Source Type: research

miR-1184 regulates inflammatory responses and cell apoptosis by targeting TRADD in an LPS-induced cell model of sepsis
Exp Ther Med. 2021 Jun;21(6):630. doi: 10.3892/etm.2021.10062. Epub 2021 Apr 15.ABSTRACTMicroRNAs (miRs) have been reported to be potential clinical biomarkers for sepsis. miR-1184 is a multifunctional microRNA that exerts roles in the development of various diseases. However, the role of miR-1184 in children with sepsis remain unknown. In the present study, THP-1 cells were stimulated with 1 µg/ml lipopolysaccharide (LPS) for 24 h to establish an in vitro sepsis model. Reverse transcription-quantitative PCR was used to evaluate the expression of miR-1184 in clinical specimens, and of IL-6, TNF-α, IL-1β, m...
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Ping Ling Rong Tang Huazhu Wang Xiuqin Deng Jianli Chen Source Type: research

MicroRNA-325 inhibits the proliferation and induces the apoptosis of T cell acute lymphoblastic leukemia cells in a BAG2-dependent manner
In conclusion, the findings of the present study demonstrated that miR-325 directly targets the BAG2 gene and that the introduction of miR-325 can accelerate apoptosis and suppress the proliferation of Jurkat cells by silencing BAG2 expression.PMID:33936287 | PMC:PMC8082601 | DOI:10.3892/etm.2021.10063 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Fengyu Wang Fengli Wang Shengyu Zhang Xiaogang Xu Source Type: research