lncRNA ST8SIA6-AS1 facilitates proliferation and invasion in liver cancer by regulating miR-142-3p
In conclusion, ST8SIA6-AS1 was indicated to exert an oncogenic function in liver cancer by competitively sponging miR-142-3p.PMID:34659494 | PMC:PMC8515546 | DOI:10.3892/etm.2021.10783 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - October 18, 2021 Category: General Medicine Authors: Yang Zhang Yan Yang Yi Zhang Zhisu Liu Source Type: research

YAP1 promotes high glucose-induced inflammation and extracellular matrix deposition in glomerular mesangial cells by modulating NF- κB/JMJD3 pathway
In conclusion, it was demonstrated that YAP1 can promote high glucose-induced inflammation and ECM deposition by activating the NF-κB/JMJD3 signaling pathway in GMCs.PMID:34659495 | PMC:PMC8515513 | DOI:10.3892/etm.2021.10784 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - October 18, 2021 Category: General Medicine Authors: Yan Wang Jinmei Xu Zhifeng Cheng Source Type: research

Care of abdominal skin in ankylosing spondylitis patients undergoing corrective spinal surgery
Exp Ther Med. 2021 Dec;22(6):1350. doi: 10.3892/etm.2021.10785. Epub 2021 Sep 23.ABSTRACTThe present study aimed to investigate the effect of a new method of abdominal skin care on patients with ankylosing spondylitis (AS) undergoing orthopedic surgery. A total of 90 patients with AS and thoracolumbar kyphosis undergoing orthopedic surgery were randomly divided into two groups. Patients in the control group received routine care while those in experimental group received the following new treatment regimen: i) Vaseline cream was applied to the abdominal skin for 3-5 min every 6 h; ii) preoperative stretch exercises were en...
Source: Experimental and Therapeutic Medicine - October 18, 2021 Category: General Medicine Authors: Xiaojing Su Zheng Wang Bing Wu Kai Song Yixiu Chen Chunzheng Zhang Chuanjuan Xue Lili Ge Yang Liu Source Type: research

Computational healthcare: Present and future perspectives (Review)
Exp Ther Med. 2021 Dec;22(6):1351. doi: 10.3892/etm.2021.10786. Epub 2021 Sep 23.ABSTRACTArtificial intelligence (AI) has been developed through repeated new discoveries since around 1960. The use of AI is now becoming widespread within society and our daily lives. AI is also being introduced into healthcare, such as medicine and drug development; however, it is currently biased towards specific domains. The present review traces the history of the development of various AI-based applications in healthcare and compares AI-based healthcare with conventional healthcare to show the future prospects for this type of care. Know...
Source: Experimental and Therapeutic Medicine - October 18, 2021 Category: General Medicine Authors: Ayumu Asai Masamitsu Konno Masateru Taniguchi Andrea Vecchione Hideshi Ishii Source Type: research

MicroRNA-218 aggravates H < sub > 2 < /sub > O < sub > 2 < /sub > -induced damage in PC12 cells via spred2-mediated autophagy
Exp Ther Med. 2021 Dec;22(6):1352. doi: 10.3892/etm.2021.10787. Epub 2021 Sep 23.ABSTRACTThe current study aimed to investigate the effects and underlying mechanism of miR-218 in H2O2-induced neuronal injury. The impacts of miR-218 knockdown on cell viability, apoptosis and autophagy-associated proteins were detected by Cell Counting Kit-8 assay, flow cytometry and western blotting in H2O2-injured PC12 cells, respectively. Reverse transcription-quantitative PCR (RT-qPCR) and western blotting was executed to explore the expression level of miR-218 and sprouty-related EVH1 domainprotein2 (spred2) in H2O2-stimulated cells. Be...
Source: Experimental and Therapeutic Medicine - October 18, 2021 Category: General Medicine Authors: Duoping Chen Chunmei Li Rui Lv Source Type: research

Effect of inner physical properties on powder adhesion in inhalation capsules in case of a high resistance device
Exp Ther Med. 2021 Dec;22(6):1353. doi: 10.3892/etm.2021.10788. Epub 2021 Sep 23.ABSTRACTThe inhalation performance of a dry powder inhaler (DPI) depends on the inhalation patterns of patients, inhalation particle characteristics and inhalation devices. In capsule-based DPIs, the capsule plays an important role in the dispersion of inhalation particles. The present study investigated the effects of inner physical properties of capsules on drug release from capsules-based DPIs with high resistance device. Atomic force microscopy (AFM) was used to evaluate the capsule physical properties, such as the capsule inner structure ...
Source: Experimental and Therapeutic Medicine - October 18, 2021 Category: General Medicine Authors: Hiroko Otake Misa Minami Mizuki Yamaguchi Sawako Akiyama Kazunori Inaba Noriaki Nagai Source Type: research

Nevi, biologics for psoriasis and the risk for skin cancer: A real concern? (Case presentation and short review)
We present the case of a 31-year-old male patient, diagnosed with psoriasis, who was deemed eligible for systemic therapy. Treatment with methotrexate was initiated, but without a satisfactory outcome. Given the patient's resistant disease involving 15% of his body surface, his desire to have a clear skin, besides his being naïve to biologic therapy, he was proposed to start treatment with secukinumab 300 mg monthly. The patient experienced complete clearance of lesions and was followed-up on the basis of clinical and biological parameters. There are limited data concerning the relationship between melanocytic lesions...
Source: Experimental and Therapeutic Medicine - October 18, 2021 Category: General Medicine Authors: Florin Ciprian Bujoreanu Laura Bezman Diana Sabina Radaschin Elena Niculet Carmen Bobeica Mihaela Craescu Thomas Nadasdy Daniela Stan Jicman Valeriu Ardeleanu Lawrence Chukwudi Nwabudike Silviu Adrian Marinescu Alin Laurentiu Tatu Source Type: research

Non-alcoholic fatty liver disease and hematologic manifestations (Review)
Exp Ther Med. 2021 Dec;22(6):1355. doi: 10.3892/etm.2021.10790. Epub 2021 Sep 23.ABSTRACTNon-alcoholic fatty liver disease (NAFLD) is a multisystem disease, and it is associated with numerous extra-hepatic manifestations or additional co-occurring diseases. The aim of the present review was the identification and management of the hematologic manifestations of NAFLD. One of the triggers is considered to be iron abnormalities. Increased ferritin levels, hepatic iron deposits and iron overload are associated with NAFLD. The iron overload degree and severity are associated with the level of liver fibrosis and with the risk fo...
Source: Experimental and Therapeutic Medicine - October 18, 2021 Category: General Medicine Authors: Vlad P ădureanu Dalia Dop Alice Nicoleta Dr ăgoescu Rodica P ădureanu Anca Emanuela Mu șetescu Lauren țiu Nedelcu Source Type: research

Fibroblast growth factor 2 suppresses the expression of C-C motif chemokine 11 through the c-Jun N-terminal kinase pathway in human dental pulp-derived mesenchymal stem cells
Exp Ther Med. 2021 Dec;22(6):1356. doi: 10.3892/etm.2021.10791. Epub 2021 Sep 24.ABSTRACTThe regulation of the mesenchymal stem cell (MSC) programming mechanism promises great success in regenerative medicine. Tissue regeneration has been associated not only with the differentiation of MSCs, but also with the microenvironment of the stem cell niche that involves various cytokines and immune cells in the tissue regeneration site. In the present study, fibroblast growth factor 2 (FGF2), the principal growth factor for tooth development, dental pulp homeostasis and dentin repair, was reported to affect the expression of cytok...
Source: Experimental and Therapeutic Medicine - October 18, 2021 Category: General Medicine Authors: Rika Kurogoushi Tomokazu Hasegawa Yuki Akazawa Kokoro Iwata Asuna Sugimoto Kimiko Yamaguchi-Ueda Aya Miyazaki Anrizandy Narwidina Keita Kawarabayashi Takamasa Kitamura Hiroshi Nakagawa Tomonori Iwasaki Tsutomu Iwamoto Source Type: research

Pharmacological effects of < em > Picrasma quassioides < /em > (D. Don) Benn for inflammation, cancer and neuroprotection (Review)
Exp Ther Med. 2021 Dec;22(6):1357. doi: 10.3892/etm.2021.10792. Epub 2021 Sep 24.ABSTRACTPicrasma quassioides (D. Don) Benn is an Asian shrub with a considerable history of traditional medicinal use. P. quassioides and its extracts exhibit good therapeutic properties against several diseases, including anti-inflammatory, antibacterial and anticancer effects. However, the composition of compounds contained in P. quassioides is complex; although various studies have examined mixtures or individual compounds extracted from it, studies on the application of P. quassioides extracts remain limited. In the present review, the str...
Source: Experimental and Therapeutic Medicine - October 18, 2021 Category: General Medicine Authors: Jaihyung Lee Yi-Xi Gong Hyunjeong Jeong Hoyoung Seo Dan-Ping Xie Hu-Nan Sun Taeho Kwon Source Type: research

CMPK2 accelerates liver ischemia/reperfusion injury via the NLRP3 signaling pathway
In conclusion, the results of the present study demonstrate that CMPK2 is indispensable for NLRP3 inflammasome activation, making CMPK2 an effective target to relieve the liver from I/R injury. In addition, the function of CMPK2 is closely associated with NLRP3 inflammasome activation, instead of AIM2.PMID:34659504 | PMC:PMC8515557 | DOI:10.3892/etm.2021.10793 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - October 18, 2021 Category: General Medicine Authors: Yunhai Luo Daofeng Zheng Tong Mou Junliang Pu Zuotian Huang Wei Chen Yuke Zhang Zhongjun Wu Source Type: research

Bioinformatic and experimental analyses of key biomarkers in pancreatic cancer
Exp Ther Med. 2021 Dec;22(6):1359. doi: 10.3892/etm.2021.10794. Epub 2021 Sep 24.ABSTRACTThe present study aimed to screen the key genes in pancreatic cancer and to explore the pathogenesis of pancreatic cancer. A total of three expression profiling datasets (GSE28735, GSE16515 and GSE15471) associated with pancreatic cancer were retrieved from the public gene chip database. The differentially expressed genes (DEGs) were screened by GEO2R and subjected to Gene Ontology (GO) and signaling pathway enrichment analysis. Furthermore, a protein interaction network was constructed. The GEPIA online database was used to screen for...
Source: Experimental and Therapeutic Medicine - October 18, 2021 Category: General Medicine Authors: Tianyu Ren Xiaofei Xue Xiaogang Wang Xingtong Zhou Shengchun Dang Source Type: research

Long non-coding RNA NEAT1 contributes to lipopolysaccharide-induced inflammation and apoptosis of human middle ear epithelial cells via regulating the miR-301b-3p/TLR4 axis
Exp Ther Med. 2021 Dec;22(6):1360. doi: 10.3892/etm.2021.10795. Epub 2021 Sep 24.ABSTRACTAcute otitis media (AOM) is a common infectious disease in children that is accompanied by signs and symptoms of middle ear inflammation and infection. Previous studies have shown that the long non-coding (lnc)RNA nuclear-enriched abundant transcript 1(NEAT1) participates in various inflammatory conditions and plays an important regulatory role. The focus of the present study was the biological function of NEAT1 and underlying molecular mechanism in lipopolysaccharide (LPS)-induced human middle ear epithelial cells (HMEECs). The expres...
Source: Experimental and Therapeutic Medicine - October 18, 2021 Category: General Medicine Authors: Zhuohui Liu Tao Lu Shumin Liu Fan Zhang Jinxiong Yang Shumin Dai Biao Ruan Ruiqing Long Source Type: research

DNCP induces the differentiation of induced pluripotent stem cells into odontoblasts by activating the Smad/p-Smad and p38/p-p38 signaling pathways
Exp Ther Med. 2021 Dec;22(6):1361. doi: 10.3892/etm.2021.10481. Epub 2021 Sep 24.ABSTRACTIn recent years, stem cells have been studied for treating tooth loss. The present study aimed to investigate the roles of dentin non-collagen protein (DNCP)-associated microenvironments in the differentiation of induced pluripotent stem cells (iPSCs) into dentin cells. iPSCs were cultured and identified by examining octamer-binding transcription-factor-4 (Oct-4) and sex-determining region-Y-2 (Sox-2) expression. iPSCs were differentiated by culturing DNCP-associated microenvironments (containing specific growth factors), and they were...
Source: Experimental and Therapeutic Medicine - October 18, 2021 Category: General Medicine Authors: Zhe Liu Aiping Zhan Sumeng Fan Lan Liao Wenwei Lian Source Type: research

Differentially expressed TUG1 and miR-145-5p indicate different severity of chronic heart failure and predict 2-year survival prognosis
Exp Ther Med. 2021 Dec;22(6):1362. doi: 10.3892/etm.2021.10796. Epub 2021 Sep 27.ABSTRACTLong non-coding RNAs (lncRNAs) and microRNAs (miRs) have critical roles in the progression of various diseases. The present study aimed to investigate the levels and clinical significance of lncRNA taurine upregulated gene 1 (TUG1) and miR-145-5p in patients with chronic heart failure (CHF) and explore their indicative value regarding disease severity. TUG1 and miR-145-5p levels were detected by reverse-transcription quantitative PCR. Correlations were examined using Pearson's correlation analysis. Receiver operating characteristic ana...
Source: Experimental and Therapeutic Medicine - October 18, 2021 Category: General Medicine Authors: Qinwei Zhu Shuanghong Li Kui Ji Hongyuan Zhou Chuanchao Luo Yana Sui Source Type: research

Comparative analysis of 0.1% cyclosporin A cationic emulsion and 0.05% cyclosporin A emulsion in murine dry eye cases with different severities
Exp Ther Med. 2021 Dec;22(6):1363. doi: 10.3892/etm.2021.10797. Epub 2021 Sep 27.ABSTRACTDry eye (DE), especially severe DE (SDE), can cause ocular surface defects and reduce the patient's quality of life. Several clinical studies have shown that 0.1% cyclosporin A cationic emulsion (CsA CE) could decrease corneal damage. However, no experimental study has reported the effect of 0.1% CsA CE on SDE. The present study aimed to compare the efficacy of 0.1% CsA CE with that of 0.05% CsA emulsion for ocular surface damage and inflammation in the cases of murine DE with different severities. Following exposure to desiccating str...
Source: Experimental and Therapeutic Medicine - October 18, 2021 Category: General Medicine Authors: Rujun Jin Ying Li Lan Li Jonghwa Kim Hyeon Jeong Yoon Kyung Chul Yoon Source Type: research

LGR6 promotes glioblastoma malignancy and chemoresistance by activating the Akt signaling pathway
Exp Ther Med. 2021 Dec;22(6):1364. doi: 10.3892/etm.2021.10798. Epub 2021 Sep 27.ABSTRACTChemoresistance is the primary cause of the poor outcome of glioblastoma multiforme (GBM) therapy. Leucine-rich repeat-containing G-protein coupled receptor 6 (LGR6) is involved in the growth and proliferation of several types of cancer, including gastric cancer and ovarian cancer. Therefore, the aim of the present study was to investigate the role of LGR6 in GBM malignancy and chemoresistance. Cell counting kit-8 and Matrigel®-Transwell assays were conducted to assess GBM cell viability and invasion. The effect of LGR6 on cell cyc...
Source: Experimental and Therapeutic Medicine - October 18, 2021 Category: General Medicine Authors: Yuan Yuan Cheng Xue Yang Xin Gao Si Xin Song Ming Feng Yang Fang Min Xie Source Type: research

MicroRNA-361 reduces the viability and migratory ability of pancreatic cancer cells via mediation of the MAPK/JNK pathway
Exp Ther Med. 2021 Dec;22(6):1365. doi: 10.3892/etm.2021.10799. Epub 2021 Sep 27.ABSTRACTPrevious research has revealed that microRNA-361 (miR-361) functions as a fundamental modulator in non-small-cell lung cancer and esophageal carcinoma. However, its involvement in pancreatic cancer (PC) is yet to be elucidated. Therefore, the present study aimed to examine the mechanism and function of miR-361 during the regulation of PC cell migration and viability. It was demonstrated that miR-361 expression decreased in PC cell lines and tissues, and the overexpression of miR-361 suppressed in vivo PC cell proliferation in mice. Mor...
Source: Experimental and Therapeutic Medicine - October 18, 2021 Category: General Medicine Authors: Juan Wang Zongjing Xie Yan Liu Weiguo Zhang Tingting Ji Source Type: research

Expression and role of interleukin-1 β and associated biomarkers in deep vein thrombosis
In conclusion, these results suggest that the high expression of IL-1β, TF, XOD and NF-κB may promote thrombus formation.PMID:34659512 | PMC:PMC8515515 | DOI:10.3892/etm.2021.10800 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - October 18, 2021 Category: General Medicine Authors: Rouzimaimaiti Zila Pai Qingbo Fang Guanglei Tian Bing Zhu Xiaohu Ge Source Type: research

Receptor-selective interleukin-4 mutein attenuates laser-induced choroidal neovascularization through the regulation of macrophage polarization in mice
Exp Ther Med. 2021 Dec;22(6):1367. doi: 10.3892/etm.2021.10801. Epub 2021 Sep 27.ABSTRACTMacrophage polarization has been recognized as an important inflammatory regulator in choroidal neovascularization (CNV). However, the mechanisms regulating macrophage activation and polarization, as well as their effects on angiogenesis and CNV, have not yet been elucidated. IL-4 is implicated in macrophage activation and exerts different functions in various diseases through several receptors. In the current study, the effect of IL-4 muteins on CNV was investigated in vivo. CNV was induced by laser coagulation in wild type mice. IL-4...
Source: Experimental and Therapeutic Medicine - October 18, 2021 Category: General Medicine Authors: Limo Gao Wenmin Jiang Haiying Liu Zhiheng Chen Yanhui Lin Source Type: research

Ding's herbal enema treats dextran sulfate sodium-induced colitis in mice by regulating the gut microbiota and maintaining the Treg/Th17 cell balance
Exp Ther Med. 2021 Dec;22(6):1368. doi: 10.3892/etm.2021.10802. Epub 2021 Sep 27.ABSTRACTDing's herbal enema (DHEP) is a traditional Chinese medicinal therapy that has been used to treat ulcerative colitis (UC) in China. The present study determined the molecular mechanism of the effect of DHEP in UC treatment. C57BL/6J mice were treated with 3.5% (w/v) dextran sulfate sodium (DSS) for 7 days to establish an animal model of colitis. The mice were divided into five groups (n=5): Control, vehicle, DHEP, mesalazine and β-sitosterol. After oral administration for 7 days, the body weight, disease activity index, histopatho...
Source: Experimental and Therapeutic Medicine - October 18, 2021 Category: General Medicine Authors: Yan-Yan Tan Yang Ding Xueping Zheng Gong-Jian Dai Su-Min Zhang Xu Yang Da-Chao Xu Peng Chen Jia-Min Zhang Jia-Ze Ma Meng Li Shi-Cai Huang Yan Liu Yu-Ting Zhang Han Xing Kang Ding Yi-Jiang Ding Source Type: research

Cutaneous sarcoidosis of the scalp unmasking systemic involvement: A case report
We present a case report concerning a single, solitary and asymptomatic lesion on the scalp.PMID:34659515 | PMC:PMC8515547 | DOI:10.3892/etm.2021.10803 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - October 18, 2021 Category: General Medicine Authors: Daniel Boda Ana Cutoiu Nona Bejenariu Constantin Caruntu Source Type: research

A rare complication of uterine leiomyomata: Severe haemoperitoneum caused by the rupture of an overlying subserosa variceal vein: A case report
Exp Ther Med. 2021 Dec;22(6):1370. doi: 10.3892/etm.2021.10804. Epub 2021 Sep 27.ABSTRACTThe rupture of a subserosa variceal vein overlying a uterine myoma is a rare complication and a less common cause of gynecologic haemoperitoneum. The literature data are scarce regarding this condition and less than 100 cases have been reported (including those occurring during pregnancy). The present case is of a 48-year-old woman, with a history of asymptomatic uterine myoma, who was hospitalised for severe abdominal pain with sudden onset and signs of hypovolemic shock. The emergency conventional imaging exams confirmed the diagnosi...
Source: Experimental and Therapeutic Medicine - October 18, 2021 Category: General Medicine Authors: Razvan Popovici Anda Pristavu Dan-Constantin Andronic Paula Zan Benedicte Riey Murarasu Mara Raluca Mogos Loredana Maria Himiniuc Mihaela Grigore Source Type: research

The Alberta Stroke Program Early CT score (ASPECTS): A predictor of mortality in acute ischemic stroke
The objective of this study was to evaluate the importance and utility of the Alberta Stroke Program Early CT Score (ASPECTS) as a mortality predictor factor in diabetic vs. non-diabetic patients with acute ischemic stroke (AIS), correlated with age, monocyte values, and high-sensitivity cardiac troponin I (hs-cTnI). The prospective longitudinal observational study included 340 patients with AIS divided into two groups: diabetics and non-diabetics. ASPECTS was evaluated within the first 24 h after admission to the center. The ASPECTS was lower in the group of diabetic patients on average 4.9 vs. 6.05 (P
Source: Experimental and Therapeutic Medicine - October 18, 2021 Category: General Medicine Authors: Nicolae Ovidiu Pop Delia Mirela Tit Camelia Cristina Diaconu Mihai Alexandru Munteanu Emilia Elena Babes Manuela Stoicescu Mircea Ioachim Popescu Simona Bungau Source Type: research

Protein tyrosine kinase 7-knockdown inhibits oral squamous cell carcinoma cell viability, proliferation, migration and invasion via downregulating dishevelled segment polarity protein 3 expression
In conclusion, the results of the present study suggested that PTK7 may be a key regulator of OSCC proliferation, migration and invasion, and PTK7-knockdown may inhibit OSCC cell viability, proliferation, invasion and migration by downregulating DVL3 expression. Therefore, PTK7 and DVL3 may represent potential biomarkers for diagnosis and treatment, as well as promising drug targets for OSCC.PMID:34659518 | PMC:PMC8515512 | DOI:10.3892/etm.2021.10806 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - October 18, 2021 Category: General Medicine Authors: Xiaoye Jin Tao Huang Caihong Ma Jiafeng Duan Rong Li Wei Zhang Wenyan Tian Source Type: research

MicroRNA-602 prevents the development of inflammatory bowel diseases in a microbiota-dependent manner
Exp Ther Med. 2021 Dec;22(6):1373. doi: 10.3892/etm.2021.10808. Epub 2021 Sep 28.ABSTRACTInflammatory bowel diseases (IBD) are a group of chronic disorders occurring in the intestinal tract. Previous studies demonstrated that genetics and microbiota play critical roles in the pathogenesis of IBD. Discoveries of genes that may regulate the homeostasis of gut microbiota and pathogenesis of IBD have the potential to provide new therapeutic targets for IBD treatment. The results suggested that the expression level of microRNA (miR)-602 is negatively related to the development of IBD, and that miR-602 overexpression in mice may...
Source: Experimental and Therapeutic Medicine - October 18, 2021 Category: General Medicine Authors: Song Zhao Lei Zhu Wan Feng Lu Zhang Dan-Dan Chen Yu-Cui Hu Hong Shen Source Type: research

Probiotics and prebiotics in atopic dermatitis: Pros and cons (Review)
Exp Ther Med. 2021 Dec;22(6):1376. doi: 10.3892/etm.2021.10811. Epub 2021 Sep 28.ABSTRACTAtopic dermatitis (AD) represents a chronic inflammatory skin condition in which the skin barrier is impaired; thus, the permeability is increased. Hence, there is a greater risk of allergic sensitization, as well as a higher pH and lower protection against resident microbes. Since this condition is currently increasing among children, it requires further study, as little is known regarding the pathogenesis that makes the skin prone to chronic relapsing inflammation. Trying to standardize the data regarding the use of prebiotics and pr...
Source: Experimental and Therapeutic Medicine - October 15, 2021 Category: General Medicine Authors: Ionut Serban Fanfaret Daniel Boda Laura Mihaela Ion Daniela Hosseyni Poliana Leru Selda Ali Sabina Corcea Roxana Bumbacea Source Type: research

Infra-hepatic caval resection en bloc with right nephrectomy followed by caval reconstruction for locally advanced caval leiomyosarcoma: A case report and literature review
We present the case of a 43-year-old patient diagnosed with a large retroperitoneal sarcoma originating from the cava vein invading the right kidney. Resection of the tumor was performed en bloc with caval resection and right nephrectomy, without reimplantation of the left renal vein at the level of the graft. Extended visceral and vascular resections might be needed in order to achieve complete resection of inferior cava vein sarcomas; re-implantation of the left renal vein being not mandatory if rich collateral circulation is present.PMID:34650625 | PMC:PMC8506944 | DOI:10.3892/etm.2021.10812 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - October 15, 2021 Category: General Medicine Authors: Vladislav Brasoveanu Ion Barbu Irina Balescu Nicolae Bacalbasa Ioan Cordos Source Type: research

Right-sided colon cancer with invasion of the duodenum or pancreas: A glimpse into our experience
In conclusion, although postoperative mortality can be significant, en bloc resection for LARCC invading the duodenum and/or pancreas offers prolonged survival in a considerable number of patients.PMID:34650626 | PMC:PMC8506938 | DOI:10.3892/etm.2021.10813 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - October 15, 2021 Category: General Medicine Authors: Nadejda Cojocari Gabriel Viorel Crihana Nicolae Bacalbasa Irina Balescu Leonard David Source Type: research

Osteoporosis therapies and their mechanisms of action (Review)
Exp Ther Med. 2021 Dec;22(6):1379. doi: 10.3892/etm.2021.10815. Epub 2021 Sep 28.ABSTRACTOsteoporosis is a common disease that affects millions of patients worldwide and is most common in menopausal women. The main characteristics of osteoporosis are low bone density and increased risk of fractures due to deterioration of the bone architecture. Osteoporosis is a chronic disease that is difficult to treat; thus, investigations into novel effective therapeutic methods are required. A number of studies have focused on determining the most effective treatment options for this disease. There are several treatment options for os...
Source: Experimental and Therapeutic Medicine - October 15, 2021 Category: General Medicine Authors: Beomchang Kim Yong Jin Cho Wonbong Lim Source Type: research

Baicalein pre-treatment alleviates hepatic ischemia/reperfusion injury in mice by regulating the Nrf2/ARE pathway
Exp Ther Med. 2021 Dec;22(6):1380. doi: 10.3892/etm.2021.10816. Epub 2021 Sep 28.ABSTRACTHepatic ischemia-reperfusion injury (HIRI) is caused by blood flow recovery following ischemia. Baicalein (BAI), a natural antioxidant used in traditional Chinese medicine, eliminates excessive free radicals and protects the structure of the cell membrane. However, its protective mechanism against HIRI is still unclear. The present study investigated underlying mechanism using a mouse HIRI model. Liver injury was evaluated using serum levels of alanine aminotransferase and aspartate aminotransferase, and hematoxylin-eosin staining was ...
Source: Experimental and Therapeutic Medicine - October 15, 2021 Category: General Medicine Authors: Yi Zhou Zhi Tan Hao Huang Yonglian Zeng Shilian Chen Jie Wei Guozhen Huang Chaosi Qian Guandou Yuan Songqing He Source Type: research

Euphornin L promotes lipid clearance by dual regulation of LDLR and PCSK9
Exp Ther Med. 2021 Dec;22(6):1381. doi: 10.3892/etm.2021.10817. Epub 2021 Sep 28.ABSTRACTOur previous study identified euphornin L as an active lipid-lowering compound in high-fat diet-fed Golden Syrian hamsters. The aim of the present study was to investigate the mechanisms underlying the lipid-lowering effects of euphornin L. Euphornin L in HepG2 cells was assessed via DiI-LDL update assays and found to increase LDL-update and LDLR protein levels. RNA interference assays demonstrated that its LDL-update effects were LDLR-dependent. Dual luciferase reporter and mRNA stability assays revealed that euphornin L had little ef...
Source: Experimental and Therapeutic Medicine - October 15, 2021 Category: General Medicine Authors: Huihui Li Jun Li Xianjing Zhang Jiaomeng Li Cong Xi Wenqiong Wang Youli Lu Lijiang Xuan Source Type: research

NEDD4 E3 ubiquitin protein ligase serves an important role in cutaneous melanoma occurrence and development
Exp Ther Med. 2021 Dec;22(6):1382. doi: 10.3892/etm.2021.10818. Epub 2021 Sep 28.ABSTRACTThe present study aimed to discuss the effects and relative mechanisms of NEDD4 E3 ubiquitin protein ligase (NEDD4) in cutaneous melanoma (CMM) occurrence and development. Clinical cancer and adjacent normal tissues samples were collected to analyze pathological changes and protein expression of NEDD4. Moreover, small interfering (si)RNA was used to knockdown NEDD4 expression in SK-MEL-2 and Malme-3M cells. Cellular proliferation, apoptosis, invasiveness and migration were examined using colony formation, flow cytometric, Transwell and...
Source: Experimental and Therapeutic Medicine - October 15, 2021 Category: General Medicine Authors: Fang Cheng Yi Cheng Xiaoling Zhao Lihui An Linfang Yang Zihan Li Lei Zhang Runzhi He Source Type: research

miR-124 promotes apoptosis and inhibits the proliferation of vessel endothelial cells through P38/MAPK and PI3K/AKT pathways, making it a potential mechanism of vessel endothelial injury in acute myocardial infarction
Exp Ther Med. 2021 Dec;22(6):1383. doi: 10.3892/etm.2021.10819. Epub 2021 Sep 29.ABSTRACTDue to its rapid onset and high rates of fatality, acute myocardial infarction (AMI) has long been one of the most fatal diseases among all types of heart diseases. Therefore, intensive research efforts have been focused on understanding AMI's potential pathogenesis to seek effective treatment options. In the present study, 20 peripheral blood samples were collected from patients with AMI, after which reverse transcription-quantitative PCR analysis revealed that microRNA (miR)-124 levels in the peripheral blood of patients with AMI was...
Source: Experimental and Therapeutic Medicine - October 15, 2021 Category: General Medicine Authors: Weimin Ma Xin Zhang Yang Liu Source Type: research

Experimental EDX analysis of different periodontal splinting systems
Exp Ther Med. 2021 Dec;22(6):1384. doi: 10.3892/etm.2021.10820. Epub 2021 Sep 29.ABSTRACTPhysiological/normal tooth mobility may be defined as the slight displacement of the clinical crown of a tooth, which is allowed by the resilience of an intact and healthy periodontium, under the application of a moderate force. The factors influencing the success and longevity of dental splinting are the type of material used for the splint, the type of composite resin, the number and location of the dental units included for splinting (maxillary or mandibular arch). In periodontology, the term 'splint' is defined as the joining of tw...
Source: Experimental and Therapeutic Medicine - October 15, 2021 Category: General Medicine Authors: Ancu ța Goriuc Alexandra Jit ăreanu Ioana M ârțu Cristina Gena Dasc ălu Diana Cristala Kappenberg-Ni ţescu Sorina-Mihaela Solomon Alexandra M ârțu Liliana Foia Iustin Țapu Bogdan Istrate Monica Tatarciuc Ionu ț Luchian Source Type: research

Clinical and surgical algorithm for managing iatrogenic bile duct injuries during laparoscopic cholecystectomy: A multicenter study
Exp Ther Med. 2021 Dec;22(6):1385. doi: 10.3892/etm.2021.10821. Epub 2021 Sep 29.ABSTRACTThe present study was a multicenter, analytical, nonrandomized research on 108 cases of intraoperative vascular and bile duct lesions during laparoscopic cholecystectomies. We selected these cases from 16,559 cholecystectomies performed entirely laparoscopically or debuted laparoscopically and converted to an open approach. The study included two surgical centers labeled as primary, with extensive experience in hepato-biliary reconstructive surgery, and four other centers labeled as secondary that referred cases to the previous two. Ou...
Source: Experimental and Therapeutic Medicine - October 15, 2021 Category: General Medicine Authors: Cosmin Moldovan Daniel Cochior Gabriel Gorecki Elena Rusu Florin-Dan Ungureanu Source Type: research

Evaluation of small airway function and its application in patients with chronic obstructive pulmonary disease (Review)
Exp Ther Med. 2021 Dec;22(6):1386. doi: 10.3892/etm.2021.10822. Epub 2021 Sep 29.ABSTRACTChronic obstructive pulmonary disease (COPD) is a chronic airway inflammatory disease characterized by incomplete reversible airflow limitation. The diagnosis of COPD is mainly based on pulmonary function examination. In recent years, it has been indicated that small airway dysfunction occurs in patients with all stages of COPD, even in high-risk smoking groups who have not yet met the diagnostic criteria for COPD. Early recognition of small airway dysfunction and early initiation of small airway targeted therapy have become foci of re...
Source: Experimental and Therapeutic Medicine - October 15, 2021 Category: General Medicine Authors: Yan Li Xin-Yang Li Li-Rong Yuan Hai-Long Wang Min Pang Source Type: research

Peutz-Jeghers syndrome: Skin manifestations and endocrine anomalies (Review)
Exp Ther Med. 2021 Dec;22(6):1387. doi: 10.3892/etm.2021.10823. Epub 2021 Sep 29.ABSTRACTPeutz-Jeghers syndrome (PJS), a rare autosomal dominant serine/threonine kinase 11 (STK11)/ liver kinase B1 (LKB1) gene-related genodermatosis, is characterized by oral hyperpigmentation (OHP); multiple gastro-intestinal mucosal benign hamartomatous polyps causing local bleeding, occlusion, intussusception, post-resection small bowel syndrome, associated increased risk of small intestinal cancer (incidence during the third decade); and 76% cumulative higher risk than the global population of developing non-gastrointestinal tumors (fema...
Source: Experimental and Therapeutic Medicine - October 15, 2021 Category: General Medicine Authors: Florica Sandru Aida Petca Mihai Cristian Dumitrascu Razvan-Cosmin Petca Mara Carsote Source Type: research

Upregulation of AQP2 mediated by transcription factor FOXO1 inhibits TGF- β-induced fibrosis in human urothelial cells
In conclusion, the transcription factor FOXO1 may upregulate AQP2 expression, thereby inhibiting TGF-β-induced fibrosis in human urothelial cells. The findings of the present study may provide a novel potential strategy for the clinical treatment of BOO by targeting AQP2.PMID:34650636 | PMC:PMC8506930 | DOI:10.3892/etm.2021.10824 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - October 15, 2021 Category: General Medicine Authors: Yue Qiu Zhaoquan Xing Zhaoxin Guo Zhaoxu Liu Source Type: research

DDX3X deficiency alleviates LPS-induced H9c2 cardiomyocytes pyroptosis by suppressing activation of NLRP3 inflammasome
Exp Ther Med. 2021 Dec;22(6):1389. doi: 10.3892/etm.2021.10825. Epub 2021 Sep 30.ABSTRACTIncreasing evidence suggest that NOD-like receptor protein 3 (NLRP3) inflammasome-mediated pyroptosis may be the underlying pathological mechanism of sepsis-induced cardiomyopathy. DDX3X, an ATP-dependent RNA helicase, plays a vital role in the formation of the NLRP3 inflammasome by directly interacting with cytoplasmic NLRP3. However, whether DDX3X has a direct impact on lipopolysaccharide (LPS)-induced cardiomyocyte injury by regulating NLRP3 inflammasome assembly remains unclear. The present study aimed to investigate the role of DD...
Source: Experimental and Therapeutic Medicine - October 15, 2021 Category: General Medicine Authors: Dandan Feng Liang Guo Jing Liu Yunxuan Song Xiuyuan Ma Haiyang Hu Ju Liu Enkui Hao Source Type: research

Monensin suppresses cell proliferation and invasion in ovarian cancer by enhancing MEK1 SUMOylation
Exp Ther Med. 2021 Dec;22(6):1390. doi: 10.3892/etm.2021.10826. Epub 2021 Sep 30.ABSTRACTOvarian cancer is the most lethal gynecologic malignancy, and is usually diagnosed at an advanced stage. Most patients relapse within 12-24 months and die from progressive chemotherapy-resistant diseases. Significant progress has been made in developing new targeted therapies for human cancer, including ovarian cancer. However, an effective alternative to drug development is to repurpose drugs. The present study investigated the possibility of reusing the antibiotic monensin as an anti-ovarian cancer drug. After applying a series of ti...
Source: Experimental and Therapeutic Medicine - October 15, 2021 Category: General Medicine Authors: Shujuan Yao Wen Wang Bin Zhou Xiujuan Cui Hui Yang Shiqian Zhang Source Type: research

Activated protein C overexpression suppresses the pyroptosis of subarachnoid hemorrhage model cells by regulating the NLRP3 inflammasome pathway
Exp Ther Med. 2021 Dec;22(6):1391. doi: 10.3892/etm.2021.10827. Epub 2021 Sep 30.ABSTRACTSubarachnoid hemorrhage (SAH) is a condition with a high associated mortality rate that is caused by hemorrhagic stroke. Activated protein C (APC) serves a neuroprotective role in central nervous system diseases. However, its role in SAH remains unclear. The present study aimed to investigate the role of APC and its regulatory mechanism in SAH. The SAH rat model was constructed through internal carotid artery puncture, while the SAH cell model was established via the application of oxygenated hemoglobin. ELISA was performed to detect t...
Source: Experimental and Therapeutic Medicine - October 15, 2021 Category: General Medicine Authors: Ai Yan Xuyan Pan Xianqiang Wen Xiaohu Nie Yuntao Li Source Type: research

Melatonin attenuates low shear stress-induced pyroptosis and endothelial cell dysfunction via the ROR α/miR-223/STAT-3 signalling pathway
Exp Ther Med. 2021 Dec;22(6):1392. doi: 10.3892/etm.2021.10828. Epub 2021 Sep 30.ABSTRACTEndothelial cells sense changes in blood flow shear stress and affect the progression of atherosclerotic plaques. Pyroptosis is an inflammatory form of cell death and has been implicated in cardiovascular diseases. Melatonin and its nuclear receptor retinoid-related orphan receptor α (RORα) have protective effects on the development of atherosclerosis. To date, whether melatonin can prevent endothelial cell pyroptosis and dysfunction in pathological shear stress remains unclear. In the present study, human umbilical vein en...
Source: Experimental and Therapeutic Medicine - October 15, 2021 Category: General Medicine Authors: Sui Yi Yang Yang Source Type: research

Zinc status, insulin resistance and glycoxidative stress in elderly subjects with type 2 diabetes mellitus
Exp Ther Med. 2021 Dec;22(6):1393. doi: 10.3892/etm.2021.10829. Epub 2021 Sep 30.ABSTRACTZinc deficiencies have been reported in numerous pathologies, such as diabetes mellitus, but also in the physiological process of ageing. Similarly, the end products of glycoxidation processes, advanced glycation end products (AGEs), are damaging compounds, a myriad of reports linking them to the development and progression of several age-associated chronic diseases. The aim of the present study was to analyze the relationships between zinc status, glycoxidative stress and insulin resistance (IR) in elderly subjects with type 2 diabete...
Source: Experimental and Therapeutic Medicine - October 15, 2021 Category: General Medicine Authors: Daniela Gr ădinaru Denisa Margin ă Anca Ungurianu Georgiana Ni țulescu C ătălina Monica Pena Constantin Ionescu-T îrgoviște Rucsandra D ănciulescu Miulescu Source Type: research

Animal models of dry eye disease: Useful, varied and evolving (Review)
Exp Ther Med. 2021 Dec;22(6):1394. doi: 10.3892/etm.2021.10830. Epub 2021 Oct 1.ABSTRACTDry eye disease (DED), which is a prevalent disease that still lacks successful treatment options, remains a major challenge in ophthalmology. Multiple animal models of DED have been used to decipher its pathophysiology and to develop novel treatments. These models use mice, rats, rabbits, cats, dogs and non-human primates. Each model assesses aspects of DED by focusing on elements of the lacrimal functional unit, which controls the homeostasis of the tear film. The present review outlines representative DED animal models and assesses t...
Source: Experimental and Therapeutic Medicine - October 15, 2021 Category: General Medicine Authors: Wei Huang Konstantinos Tourmouzis Henry Perry Robert A Honkanen Basil Rigas Source Type: research

Tat-thioredoxin 1 reduces inflammation by inhibiting pro-inflammatory cytokines and modulating MAPK signaling
Exp Ther Med. 2021 Dec;22(6):1395. doi: 10.3892/etm.2021.10831. Epub 2021 Oct 1.ABSTRACTThioredoxin 1 (Trx1) serves a central role in redox homeostasis. It is involved in numerous other processes, including oxidative stress and apoptosis. However, to the best of our knowledge, the role of Trx1 in inflammation remains to be explored. The present study investigated the function and mechanism of cell permeable fused Tat-Trx1 protein in macrophages and a mouse model. Transduction levels of Tat-Trx1 were determined via western blotting. Cellular distribution of transduced Tat-Trx1 was determined by fluorescence microscopy. 2',7...
Source: Experimental and Therapeutic Medicine - October 15, 2021 Category: General Medicine Authors: Eun Ji Yeo Min Jea Shin Hyeon Ji Yeo Yeon Joo Choi Eun Jeong Sohn Lee Re Lee Hyun Jung Kwon Hyun Ju Cha Sung Ho Lee Sunghou Lee Yeon Hee Yu Duk-Soo Kim Dae Won Kim Jinseu Park Kyu Hyung Han Won Sik Eum Soo Young Choi Source Type: research

Diagnostic value and significance of serum miR-132 combined with miR-223 for sepsis-induced cardiomyopathy
Exp Ther Med. 2021 Dec;22(6):1396. doi: 10.3892/etm.2021.10832. Epub 2021 Oct 1.ABSTRACTIn previous studies, miR-132 and miR-223 were considered to be involved in cellular and pathological processes of diseases. However, the role of early diagnosis and prognosis evaluation in sepsis-induced cardiomyopathy (SIC) remains unknown. The present study aimed to explore the diagnostic value of combined detection of miR-132 and miR-223 for SIC and their correlation with creatine kinase-MB (CK-MB), cardiac troponin I (cTnI), tumor necrosis factor α (TNF-α), and interleukin-6 (IL)-6. SIC patients (n=80) admitted to Tianji...
Source: Experimental and Therapeutic Medicine - October 15, 2021 Category: General Medicine Authors: Yanping Li Bin Lu Muming Yu Jianhua Zhai Ying Yao Yanfen Chai Source Type: research

Heparin alleviates LPS-induced endothelial injury by regulating the TLR4/MyD88 signaling pathway
Exp Ther Med. 2021 Dec;22(6):1397. doi: 10.3892/etm.2021.10833. Epub 2021 Oct 1.ABSTRACTHeparin is a commonly used in the clinic, however, Heparin's effect on endothelial injury remains unclear. The aim of the present study was to evaluate the effects and possible mechanisms of action underlying heparin treatment in lipopolysaccharide (LPS)-induced endothelial injury in vitro. TNF-α, IL-1β, IL-6 and IFN-γ levels were measured using ELISA. Cell proliferation was measured using a 5-ethynyl-2'-deoxyuridine (EdU) assay. The number of apoptotic cells and apoptotic rate were evaluated using TUNEL assays and flow...
Source: Experimental and Therapeutic Medicine - October 15, 2021 Category: General Medicine Authors: Wenxun Liu Yan Li Zhaozhao Wu Kerong Hai Yun Wang Xiaohong Zhou Qingshan Ye Source Type: research

Roflumilast, a type of phosphodiesterase 4 inhibitor, can reduce intestinal injury caused by sepsis
Exp Ther Med. 2021 Dec;22(6):1398. doi: 10.3892/etm.2021.10834. Epub 2021 Oct 1.ABSTRACTSepsis is a systemic inflammatory response syndrome caused by infection, which has a complex mechanism. The gastrointestinal tract is commonly the first organ affected by sepsis, but intestinal disease itself can also induce sepsis. Roflumilast has been found to exert anti-inflammatory effects and, thus, the present study sought to examine its effect on intestinal damage caused by sepsis. In vivo studies were conducted using cecal ligation and puncture rat models, and in vitro experiments were performed using IEC-6 cells. The intestinal...
Source: Experimental and Therapeutic Medicine - October 15, 2021 Category: General Medicine Authors: Zhongyuan Zhang Meifeng Liang Xiongfei Wan Source Type: research

DUSP4 alleviates LPS-induced chondrocyte injury in knee osteoarthritis via the MAPK signaling pathway
Exp Ther Med. 2021 Dec;22(6):1401. doi: 10.3892/etm.2021.10837. Epub 2021 Oct 1.ABSTRACTKnee osteoarthritis (KOA) is characterized by cartilage damage, and the associated pathogenesis is complex. The expression of dual specificity protein phosphatase 4 (DUSP4) is significantly decreased in osteoarthritis (OA); however, the specific role and mechanism underlying DUSP4 in OA are yet to be elucidated. ATDC5 cells were treated with lipopolysaccharide (LPS) to establish the cell injury model. The expression levels of DUSP4 were decreased in OA chondrocytes, demonstrated by reverse transcription-quantitative PCR and western blot...
Source: Experimental and Therapeutic Medicine - October 15, 2021 Category: General Medicine Authors: Zhengnan Li Bojie Chen Source Type: research