Effect of arsenic trioxide on cervical cancer and its mechanisms.
Authors: Zhang L, Zhou Y, Kong J, Zhang L, Yuan M, Xian S, Wang Y, Cheng Y, Yang X Abstract Cervical cancer is one of the most common types of gynecological tumor, and thus identifying complementary or substitute treatment methods to treat cervical cancer is important. The present study aimed to evaluate the effect of arsenic trioxide (ATO), a traditional Chinese medicine, on cervical cancer cells and its underlying mechanism. MTT, colony formation and Transwell assays were performed to investigate the effects of different concentrations of ATO on cell proliferation and invasion, respectively. Western blotting and ...
Source: Experimental and Therapeutic Medicine - October 27, 2020 Category: General Medicine Tags: Exp Ther Med Source Type: research

HIPK2 sustains inflammatory cytokine production by promoting endoplasmic reticulum stress in macrophages.
Authors: Xu L, Fang H, Xu D, Wang G Abstract Uncontrolled inflammatory cytokine production by macrophages contributes to numerous conditions, including infection, endotoxemia and sepsis. A previous study proposed that endoplasmic reticulum (ER) stress acts as an essential process in inflammatory cytokine production by macrophages. The present study used a mouse sepsis model and in vitro macrophages to demonstrate that homeodomain-interacting protein kinase 2 (HIPK2) sustained cytokine production in an ER stress-dependent manner. HIPK2 expression was upregulated in the early phase of lipopolysaccharide stimulation. ...
Source: Experimental and Therapeutic Medicine - October 27, 2020 Category: General Medicine Tags: Exp Ther Med Source Type: research

miR-1225-5p inhibits non-small cell lung cancer cell proliferation, migration and invasion, and may be a prognostic biomarker.
In conclusion, the findings of the present study indicated that the downregulated expression levels of miR-1225-5p in NSCLC may predict a poor prognosis in patients and suggested miR-1225-5p as a potential therapeutic target for NSCLC. PMID: 33101465 [PubMed] (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - October 27, 2020 Category: General Medicine Tags: Exp Ther Med Source Type: research

Small interfering RNA-mediated knockdown of KRT80 suppresses colorectal cancer proliferation.
In conclusion, the findings of the present study suggested that KRT80 may be overexpressed in CRC tissues. Furthermore, KRT80 may be involved in the proliferation of CRC cells, which is likely through its ability to regulate the cell cycle and DNA replication pathways, thus it may serve as a potential therapeutic target for patients with CRC. PMID: 33101466 [PubMed] (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - October 27, 2020 Category: General Medicine Tags: Exp Ther Med Source Type: research

Antiepileptic effects of exogenous β-hydroxybutyrate on kainic acid-induced epilepsy.
Antiepileptic effects of exogenous β-hydroxybutyrate on kainic acid-induced epilepsy. Exp Ther Med. 2020 Dec;20(6):177 Authors: Si J, Wang Y, Xu J, Wang J Abstract The aim of the present study was to explore the potential anticonvulsant effects of β-hydroxybutyrate (BHB) in a kainic acid (KA)-induced rat epilepsy model. The KA-induced rat seizure model was established and BHB was administrated intraperitoneally at a dose of 4 mmol/kg 30 min prior to KA injection. Hippocampal tissues were then obtained 1, 3 and 7 days following KA administration, following which the expression levels of neuron...
Source: Experimental and Therapeutic Medicine - October 27, 2020 Category: General Medicine Tags: Exp Ther Med Source Type: research

Endometrial changes in estrogen and progesterone receptor expression during implantation in an oocyte donation program.
Authors: Klonos E, Katopodis P, Karteris E, Papanikolaou E, Tarlatzis B, Pados G Abstract Implantation is the final and most important stage of embryogenesis and is of paramount importance in achieving a successful pregnancy. Progesterone and estrogen are steroid hormones responsible for the regulation of the implantation window and the current study hypothesised that their receptors may be implicated in women undergoing oocyte donation. A total of 15 women aged 25-32 years old (mean ± SD, 28.9±2.89) undergoing oocyte donation were recruited into the present study. Participants underwent ovarian stimu...
Source: Experimental and Therapeutic Medicine - October 27, 2020 Category: General Medicine Tags: Exp Ther Med Source Type: research

SIRT1 promotes pulmonary artery endothelial cell proliferation by targeting the Akt signaling pathway.
Authors: Xi L, Ruan L, Yao X, Zhang D, Yuan H, Li Q, Yan C Abstract Pulmonary arterial hypertension (PAH) is a disease characterized by a progressive increase in pulmonary vascular resistance and obliterative pulmonary vascular remodeling; however, the pathogenesis of the disease is not completely understood. Sirtuin 1 (SIRT1) is a histone deacetylase involved in cell survival and metabolism. The present study explored the potential role of SIRT1 in human pulmonary arterial endothelial cells (HPAECs) under hypoxic conditions. In vitro HPAECs were cultured and exposed to hypoxic conditions. Subsequently, SIRT1 expre...
Source: Experimental and Therapeutic Medicine - October 27, 2020 Category: General Medicine Tags: Exp Ther Med Source Type: research

Immune cell infiltration and related core genes expression characteristics in eosinophilic and non-eosinophilic chronic rhinosinusitis with nasal polyps.
Authors: Xiong G, Xie X, Wang Q, Zhang Y, Ge Y, Lin W, Li M Abstract Chronic rhinosinusitis with nasal polyps (CRSwNP) refers to chronic inflammation of the sinonasal mucosa. It can either be eosinophilic (ECRSwNP) or non-eosinophilic (non-ECRSwNP). However, immune cell infiltration in the microenvironment and pathogenesis of ECRSwNP and non-ECRSwNP are still unclear. The aim of the present study was to assess the immune cell infiltration and molecular mechanisms of ECRSwNP and non-ECRSwNP. In the present study, 22 immune cell types in ECRSwNP and non-ECRSwNP were investigated by CIBERSORT based on transcriptome da...
Source: Experimental and Therapeutic Medicine - October 27, 2020 Category: General Medicine Tags: Exp Ther Med Source Type: research

Associated risk factors of lower limb lymphedema after treatment of cervical and endometrial cancer.
Authors: Rebegea LF, Stoleriu G, Manolache N, Serban C, Craescu M, Lupu MN, Voinescu DC, Firescu D, Ciobotaru OR Abstract The scope of the study was to identify the associated risk factors of lower limb lymphedema development in cervical and endometrial cancer patients. We retrospectively analysed 326 patients: 186 cases (57.06%) with cervical cancer and 140 cases (42.94%) with endometrial cancer were treated in Surgery, Radiotherapy, Oncology and Gynaecology Clinics of 'St. Apostle Andrew' Emergency Clinical Hospital Galati over 9 years. Adjuvant radiotherapy was performed in 83.57% of endometrial cancer cases. Ad...
Source: Experimental and Therapeutic Medicine - October 27, 2020 Category: General Medicine Tags: Exp Ther Med Source Type: research

Influence of unilateral intravitreal bevacizumab injection on the incidence of symptomatic choroidal neovascularization in the fellow eye in patients with neovascular age-related macular degeneration (Review).
Authors: Branisteanu DC, Branisteanu DE, Feraru CI, Branisteanu CI, Moraru A, Zemba M, Balta F Abstract Neovascular age-related macular degeneration (neovascular ARMD) represents only 10% of ARMD cases but is responsible, if untreated, for quick and severe central vision loss due to major macular changes. The presence of choroidal neovascularization (CNV) in one eye is associated with an approximately 10% risk of CNV development in the fellow eye each year. Intravitreal anti-VEGF therapy has quickly evolved as the standard treatment in neovascular ARMD in the last decade due to significant anatomical and functional...
Source: Experimental and Therapeutic Medicine - October 27, 2020 Category: General Medicine Tags: Exp Ther Med Source Type: research

Outcomes of simultaneous vs. sequential pars plana vitrectomy and cataract surgery.
Authors: Moraru AD, Costin D, Moraru RL, Branisteanu DC Abstract The coexistence of cataract and vitreoretinal diseases is common in clinical practice. The technological achievements of the last decade in both types of surgeries, allow simultaneous interventions on lens and retina. This retrospective study assessed the morphofunctional results obtained after performing simultaneous surgeries in a series of 87 patients presenting with both pathologies. The cases were retrospectively reviewed from their clinical records from 2017 to 2019. The patients were divided into two groups: group 1 comprising 41 cases underwen...
Source: Experimental and Therapeutic Medicine - October 27, 2020 Category: General Medicine Tags: Exp Ther Med Source Type: research

Measurement of skin viscoelasticity: A non-invasive approach in allergic contact dermatitis.
Authors: Constantin MM, Bucur S, Serban ED, Olteanu R, Bratu OG, Constantin T Abstract Non-invasive bioengineering technologies are constantly being developed, as they can provide useful insights and contribute to the improvement of medical care and scientific education. The purpose of this study was to assess skin viscoelasticity using the suction chamber method in patients with allergic contact dermatitis vs. healthy subjects, before and after applying a moisturizer safety testing cream. This was a prospective controlled study over a 3-year period (March 2016-March 2019), with 81 subjects being divided in two bal...
Source: Experimental and Therapeutic Medicine - October 27, 2020 Category: General Medicine Tags: Exp Ther Med Source Type: research

Effect of inulin in the treatment of irritable bowel syndrome with constipation (Review).
Authors: Bărboi OB, Ciortescu I, Chirilă I, Anton C, Drug V Abstract At present, irritable bowel syndrome (IBS) is a common medical problem all over the world that implies considerable social burden and high costs. Considering the different pathophysiological pathways, unitary management for IBS is not possible. Of the therapeutic approaches that have been proposed so far, only a few have been demonstrated to have beneficial effects in IBS patients. The implication of gut microbiota in IBS is obvious, similarly to the therapeutic effect of pro-/prebiotics, which is reflected by the latest publications. The intake...
Source: Experimental and Therapeutic Medicine - October 27, 2020 Category: General Medicine Tags: Exp Ther Med Source Type: research

Non-sexual HPV transmission and role of vaccination for a better future (Review).
Authors: Petca A, Borislavschi A, Zvanca ME, Petca RC, Sandru F, Dumitrascu MC Abstract Human papilloma viruses (HPVs) belong to the Papillomaviridae family and are epitheliotropic infecting squamous epithelia (skin and mucosae). HPV is estimated to be the cause of 99% of cervical cancers (there is no evidence of significant genetic predisposition for cervical cancer), 90% of anal cancer, 65% vaginal cancers, 50% vulvar cancers, and 45-90% oropharyngeal cancers. The route of HPV transmission is primarily through skin-to-skin or skin-to-mucosa contact. Sexual transmission is the most documented, but there have been ...
Source: Experimental and Therapeutic Medicine - October 27, 2020 Category: General Medicine Tags: Exp Ther Med Source Type: research

Assessment of sun-related behavior and serum vitamin D in basal cell carcinoma: Preliminary results.
Authors: Vornicescu C, Ungureanu L, Șenilă SC, Vesa ȘC, Cosgarea R, Baican CI, Mihu MC Abstract Ultraviolet radiation (UV), mainly from sunlight, is the main risk factor of the most common human skin cancer, basal cell carcinoma (BCC). A positive effect of UV on the skin is its contribution to the synthesis of vitamin D, which is important in sustaining general health, but having high levels of vitamin D is considered a risk factor for BCC. However, vitamin D receptor has antagonistic effects to UV radiation in regulating Sonic Hedgehog pathway. This is a pilot study aimed at characterizing the sun-related behav...
Source: Experimental and Therapeutic Medicine - October 27, 2020 Category: General Medicine Tags: Exp Ther Med Source Type: research

Role of osteodensitometry in osteoporosis and osteopenia in psoriatic arthritis.
This study did not emphasize an association between the patient type and the presented bone demineralization type (χ2 (2)=4.473, P=0.107) in men. In premenopausal women, there was a significant association between the patient type and the presented bone demineralization type (χ2 (2)=8.778, P=0.012), as the female patients with psoriatic arthritis presented total and partial demineralization to a larger degree, compared with healthy individuals. In menopausal women, this study did not highlight an association between the patient type and the presented bone demineralization type (χ2 (2)=4.523, P=0.104). Bone demi...
Source: Experimental and Therapeutic Medicine - October 27, 2020 Category: General Medicine Tags: Exp Ther Med Source Type: research

Correlation between quality of life and disease severity of pediatric patients with atopic dermatitis.
This study assessed the QoL of patients with AD by following parameters such as itching, disturbance of sleep, disturbance of play activity, and community activities. The study included 64 patients clinically diagnosed with AD, aged 0 to 16 years. QoL of patients with AD was measured by evaluating the QoL indices for infants and young children (IDQoL-Infants' Dermatitis Quality of Life Index) and the QoL for children over 4 years (CDQoL-Children's Dermatology Life Quality Index). The severity of the disease was also monitored by evaluating the SCORAD index. The major symptom for atopic dermatitis, pruritus, was found most ...
Source: Experimental and Therapeutic Medicine - October 27, 2020 Category: General Medicine Tags: Exp Ther Med Source Type: research

Prediction of NAFLD occurrence in prediabetes patients.
This study identified the prevalence of NAFLD, predictors of NAFLD and explored the link between insulin sensitivity, insulin resistance and leptinemia in 143 patients registered with prediabetes. Abdominal ultrasound was performed, and fasting insulin, postprandial insulin, leptin levels, common clinical/biochemical determinations were assessed. Certain variables that can predict NAFLD existence were determined and it was found that there is a high prevalence of NAFLD in patients with prediabetes. In univariate analysis, statistically significant associations (P
Source: Experimental and Therapeutic Medicine - October 27, 2020 Category: General Medicine Tags: Exp Ther Med Source Type: research

Hydroxyurea-induced superinfected ulcerations: Two case reports and review of the literature.
Authors: Iancu GM, Ocneanu A, Rotaru M Abstract The chronic use of hydroxyurea (HU) in some oncologic and non-oncologic diseases (psoriasis, sickle cell anemia) can be accompanied by side effects, both systemic and mucocutaneous. The most severe adverse events known in HU therapy are leg ulcers and cutaneous carcinomas. At skin level may also appear: xerosis, persistent pruritus, skin color changes (erythema, hyperpigmentation), cutaneous atrophy. Likewise, oral ulcerations and stomatitis may occur at mucosal level. Hair damage can be expressed through alopecia and nail damage through melanonychia and oncycholysis....
Source: Experimental and Therapeutic Medicine - October 27, 2020 Category: General Medicine Tags: Exp Ther Med Source Type: research

Antimicrobial peptides as an argument for the involvement of innate immunity in psoriasis (Review).
Authors: Alecu M, Coman G, Mușetescu A, Coman OA Abstract Antimicrobial peptides (AMPs) are a group of oligopeptides found in most multicellular organisms with a capacity for rapid and nonspecific destruction of pathogens. The action of destroying pathogens is associated with a strong proinflammatory activity, stimulating the secretion of cytokines, chemokines, growth factors but also chemotaxis, the activation of dendritic cells and involving adaptive immunity also. The action of AMPs fits perfectly into the characteristics of innate immunity which makes these peptides candidates to be considered as an important ...
Source: Experimental and Therapeutic Medicine - October 27, 2020 Category: General Medicine Tags: Exp Ther Med Source Type: research

Left heart hypoplasia operated using double pulmonary arterial banding with double arterial duct stenting: A case report.
In conclusion, the initial cardiac surgical reconstruction consisted of a mixed technique, and anticoagulant medical treatment with heparin, antibiotics (bacterial endocarditis prophylaxis to be performed throughout life); postintervention hypoxic and infectious complications resulted in multiorgan failure and death. PMID: 33101483 [PubMed] (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - October 27, 2020 Category: General Medicine Tags: Exp Ther Med Source Type: research

COVID-19 and diabetes mellitus: Unraveling the hypotheses that worsen the prognosis (Review).
Authors: Albulescu R, Dima SO, Florea IR, Lixandru D, Serban AM, Aspritoiu VM, Tanase C, Popescu I, Ferber S Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and the caused disease - coronavirus disease 2019 (COVID-19), has affected so far>6,000,000 people worldwide, with variable grades of severity, and has already inflicted>350,000 deaths. SARS-CoV-2 infection seems severely affected by background diseases such as diabetes mellitus and its related complications, that seem to be favoring the most severe manifestations of SARS-CoV-2 and, therefore, require special attention in c...
Source: Experimental and Therapeutic Medicine - October 27, 2020 Category: General Medicine Tags: Exp Ther Med Source Type: research

Ophiopogonin D attenuates PM2.5-induced inflammation via suppressing the AMPK/NF- κB pathway in mouse pulmonary epithelial cells.
Ophiopogonin D attenuates PM2.5-induced inflammation via suppressing the AMPK/NF-κB pathway in mouse pulmonary epithelial cells. Exp Ther Med. 2020 Dec;20(6):139 Authors: Wang Y, Li D, Song L, Ding H Abstract Exposure to fine particulate matter, such as particulate matter of ≤2.5 µm in diameter (PM2.5), causes pulmonary inflammation and injury to other organs. It has been reported that Ophiopogonin D (OP-D) has anti-inflammatory activity. The aim of the present study was to investigate this anti-inflammatory activity of OP-D on PM2.5-induced acute airway inflammation and its underlying ...
Source: Experimental and Therapeutic Medicine - October 24, 2020 Category: General Medicine Tags: Exp Ther Med Source Type: research

Telmisartan alleviates collagen type III glomerulopathy: A case report with literature review.
Authors: Guo Q, Liu L, Nie P, Luo P Abstract Collagen type III is commonly detected in the renal interstitium and vasculature; however, it is absent in healthy glomeruli. Deposition of collagen type III in the glomerular mesangium and capillary basement membranes may arise in two rare diseases, namely collagen type III glomerulopathy (CG) and nail patella syndrome. CG is a rare glomerular disease with no specific treatment, although supportive measures for control of hypertension and edema may help to relieve symptoms. With progression to end-stage renal disease, patients with CG may come to require dialysis and/or...
Source: Experimental and Therapeutic Medicine - October 24, 2020 Category: General Medicine Tags: Exp Ther Med Source Type: research

Expression and predictive value of HIF-1 α and VEGF in patients with burns following treatment.
Expression and predictive value of HIF-1α and VEGF in patients with burns following treatment. Exp Ther Med. 2020 Dec;20(6):141 Authors: Liu Z, Liu L, Cheng X, Gao L Abstract The present study aimed to investigate the expression and predictive value of serum hypoxia-inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF) in patients with burns following treatment. A total of 84 patients with burns treated in Jinan City People's Hospital (Jinan, China) between June 2015 and August 2017 were selected and their clinical information was collected. The expression levels ...
Source: Experimental and Therapeutic Medicine - October 24, 2020 Category: General Medicine Tags: Exp Ther Med Source Type: research

Schisandrin B exerts hypnotic effects in PCPA-treated rats by increasing hypothalamic 5-HT and γ-aminobutyric acid levels.
Schisandrin B exerts hypnotic effects in PCPA-treated rats by increasing hypothalamic 5-HT and γ-aminobutyric acid levels. Exp Ther Med. 2020 Dec;20(6):142 Authors: Wang M, Li N, Jing S, Wang C, Sun J, Li H, Liu J, Chen J Abstract Schisandrin B (SchB) is one of the primary active components of Schisandra chinensis (Turcz.) Baill., a traditional Chinese herb that has been used to treat insomnia for hundreds of years. Our previous studies revealed that SchB exerts sedative and hypnotic effects, increasing the content of γ-aminobutyric acid (GABA) and the expression of its receptors in the bra...
Source: Experimental and Therapeutic Medicine - October 24, 2020 Category: General Medicine Tags: Exp Ther Med Source Type: research

Correlation between prostate-specific antigen and four renal function-associated parameters in different ethnicities in southwest China.
Authors: Li R, Tang H, Bao T, Yang H, Jin T Abstract A limited number of studies have investigated the significance of cystatin C, creatinine, uric acid and urea in prostate cancer. The present study aimed to explore the correlation between these molecules and total prostate-specific antigen (tPSA) levels using big data from patients of different Chinese ethnicities. Patients undergoing physical examination at the Medical Examination Center of West China Hospital (Chengdu, China) between January 2010 and May 2019 were retrospectively included. A χ2 test or Fisher's test and Kruskal-Wallis rank-sum test were use...
Source: Experimental and Therapeutic Medicine - October 24, 2020 Category: General Medicine Tags: Exp Ther Med Source Type: research

Expression profiles of long non-coding RNAs during fetal lung development.
Authors: Shen JX, Bao ZD, Zhu W, Ma CL, Shen YQ, Kan Q, Zhou XG, Yang Y, Zhou XY Abstract With advances in neonatology, a greater percentage of premature infants now survive and consequently, diseases of lung development, including bronchopulmonary dysplasia and neonatal respiratory distress syndrome, have become more common. However, few studies have addressed the association between fetal lung development and long non-coding RNA (lncRNA). In the present study, right lung tissue samples of fetuses at different gestational ages were collected within 2 h of the induction of labor in order to observe morphological di...
Source: Experimental and Therapeutic Medicine - October 24, 2020 Category: General Medicine Tags: Exp Ther Med Source Type: research

Human umbilical cord-derived mesenchymal stem cells and human cord blood mononuclear cells protect against cisplatin-induced acute kidney injury in rat models.
In conclusion, the present study indicated that hCBMNCs exert similar protective effects to hUCMSCs on CP-induced AKI. hUCMSCs and hCBMNCs protect against CP-induced AKI by suppressing HMGB1 expression and preventing cell apoptosis. PMID: 33093883 [PubMed] (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - October 24, 2020 Category: General Medicine Tags: Exp Ther Med Source Type: research

Comparison of clinical efficacy of oxcarbazepine and lamotrigine combined with escitalopram, and impact on prognostic quality of life in treating patients with epilepsy and depressive disorder.
This study aimed to investigate the clinical efficacy of oxcarbazepine and lamotrigine combined with escitalopram in treating patients with epilepsy and depressive disorder, and their influence on the prognostic quality of life. A total of 108 patients with epilepsy and depression were selected as research participants. Among them, 53 patients were treated by oxcarbazepine combined with escitalopram (group A) and 55 patients were treated by lamotrigine combined with escitalopram (group B). Following six-month treatment, efficacy, epilepsy frequency and duration, Hamilton Depression Rating (HAMD) and Montgomery-Asberg Depre...
Source: Experimental and Therapeutic Medicine - October 24, 2020 Category: General Medicine Tags: Exp Ther Med Source Type: research

Circadian clock gene Period2 suppresses human chronic myeloid leukemia cell proliferation.
Authors: Wang N, Mi M, Wei X, Sun C Abstract Circadian clock genes (CCGs) are reported to serve pivotal roles in regulating the development of certain tumors, including lung cancer and colon cancer . However, their expression patterns and function in chronic myeloid leukemia (CML) remains poorly understood. The present study aimed to investigate the expression and function of circadian clock gene Period2 (Per2) in human CML. Per2 expression levels in neutrophils isolated from patients with CML and healthy donors were measured via reverse transcription-quantitative PCR. Subsequently, through lentivirus transduction,...
Source: Experimental and Therapeutic Medicine - October 24, 2020 Category: General Medicine Tags: Exp Ther Med Source Type: research

Neural stem cells induce M2 polarization of macrophages through the upregulation of interleukin-4.
Authors: Ji Z, Jiang X, Li Y, Song J, Chai C, Lu X Abstract Macrophages are divided into two types: M1- and M2-type macrophages. Both types of macrophages serve important roles during the process of inflammation. M1-type macrophages release various pro-inflammatory cytokines, such as IL-1, IFN-γ and other inflammatory mediators, such as nitric oxide, glutamate and reactive oxygen species to generate inflammation. In contrast, M2-type macrophages counteract the pro-inflammatory M1 conditions and promote tissue repair by secreting anti-inflammatory cytokines, such as IL-10. In spinal cord injury (SCI), an imbal...
Source: Experimental and Therapeutic Medicine - October 24, 2020 Category: General Medicine Tags: Exp Ther Med Source Type: research

Metformin prevents the development of monocrotaline-induced pulmonary hypertension by decreasing serum levels of big endothelin-1.
Authors: Yoshida T, Matsuura K, Goya S, Ma D, Shimada K, Kitpipatkun P, Namiki R, Uemura A, Suzuki K, Tanaka R Abstract Pulmonary hypertension (PH) is a disease with poor prognosis, and it is characterized by the progressive elevation of pulmonary vascular resistance and pressure. Various factors are associated with the pathology of PH, including AMP-activated protein kinase (AMPK) deficiency. The present study aimed to evaluate the therapeutic effect of metformin, an AMPK activator, in a monocrotaline (MCT)-induced PH rat model. Rats were randomly divided into the following three groups: i) Saline-injected group (...
Source: Experimental and Therapeutic Medicine - October 24, 2020 Category: General Medicine Tags: Exp Ther Med Source Type: research

Thrombospondin-2 is upregulated in patients with aortic dissection and enhances angiotensin II-induced smooth muscle cell apoptosis.
Authors: Qi L, Wu K, Shi S, Ji Q, Miao H, Bin Q Abstract Thrombospondin-2 (TSP-2) is an important extracellular matrix protein that is involved in a variety of cardiovascular diseases, including viral myocarditis and abdominal aortic aneurysm. The present study aimed to investigate TSP-2 expression in patients with aortic dissection (AD). Aortas were obtained from patients with AD and healthy donors, and TSP-2 expression level in all samples was measured by western blotting and immunofluorescence assays. Blood samples were also collected from patients with AD and non-AD (NAD) subjects. Circulating TSP-2, tumor necr...
Source: Experimental and Therapeutic Medicine - October 24, 2020 Category: General Medicine Tags: Exp Ther Med Source Type: research

Suppressive effects of RASAL2 on renal cell carcinoma via SOX2/ERK/p38 MAPK pathway.
Authors: Wang S, Hao X, He S, Liu C, Wang Q Abstract Metastatic renal cell carcinoma (RCC) is associated with poor prognosis. Ras protein activator like 2 (RASAL2) protein has been previously demonstrated to serves as a tumor suppressor in a variety of malignancies. Therefore, the aim of the present study was to investigate the role of RASAL2 in RCC. Reverse transcription-quantitative PCR, western blot analysis and immunohistochemistry were performed to measure mRNA and protein expression in RCC tissues, whilst immunofluorescence and western blotting were performed to evaluate protein expression in RCC cells. A Cel...
Source: Experimental and Therapeutic Medicine - October 24, 2020 Category: General Medicine Tags: Exp Ther Med Source Type: research

A compound heterozygous mutation of the alkaline phosphatase ALPL gene causes hypophosphatasia in a Han Chinese family.
Authors: Huang H, Wang J, Liang Y, Wei X, Guo D, Sun H, Zhang X, Xu X, Xiong F Abstract Hypophosphatasia (HPP) is a rare hereditary systemic disease that is characterized by defective bone and/or dental mineralization, and is caused by mutations in the alkaline phosphatase gene (ALPL). The present study investigated the ALPL mutation in a Chinese Han family with HPP and studied the pathogenesis of the mutations of the ALPL gene. DNA was extracted from peripheral venous blood of the family members. Sanger sequencing was used to screen the mutations. Associations between pathogenesis for both mutations were analyzed ...
Source: Experimental and Therapeutic Medicine - October 24, 2020 Category: General Medicine Tags: Exp Ther Med Source Type: research

Comparison of two regimens for patients with thyroid-associated ophthalmopathy receiving intravenous methyl prednisolone: A single center prospective randomized trial.
Authors: Mu PW, Tang XX, Wang YN, Lin S, Wang MM, Yin QL, Shu J, Zhu BL, Li JR, Zhou L, Zeng LY, Chen YM Abstract Intravenous (i.v.) glucocorticoid is recommended for active moderate-to-severe thyroid-associated ophthalmopathy (TAO). However, the details of the treatment schedule are still debatable. The present prospective randomized trial was performed to compare clinical outcomes and serum cytokines between the two regimens. A cohort of 90 patients with active moderate-to-severe TAO was randomized to receive i.v. methyl prednisolone on a weekly protocol or daily scheme. The response rate was evaluated at the 12-...
Source: Experimental and Therapeutic Medicine - October 24, 2020 Category: General Medicine Tags: Exp Ther Med Source Type: research

TUG1 knockdown promoted viability and inhibited apoptosis and cartilage ECM degradation in chondrocytes via the miR-17-5p/FUT1 pathway in osteoarthritis.
In conclusion, TUG1 knockdown inhibited OA progression by downregulating FUT1 via miR-17-5p. PMID: 33093892 [PubMed] (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - October 24, 2020 Category: General Medicine Tags: Exp Ther Med Source Type: research

Inhibition of miRNA-29a regulates intestinal barrier function in diarrhea-predominant irritable bowel syndrome by upregulating ZO-1 and CLDN1.
In conclusion, the present study revealed that miRNA-29a is involved in the pathogenesis of IBS-D, probably by downregulating ZO-1 and CLDN1 expression, suggesting that miRNA-29a is likely to be an important regulator of intestinal barrier function and could be a possible therapeutic target for IBS-D. PMID: 33093893 [PubMed] (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - October 24, 2020 Category: General Medicine Tags: Exp Ther Med Source Type: research

A modified protocol for the isolation, culture, and cryopreservation of rat embryonic neural stem cells.
Authors: Zhou H, Yang H, Lu L, Li X, Pan B, Fu Z, Chu T, Liu J, Kang Y, Liu L, Ning G, Ding W, Wu P, Kong X, Feng S Abstract Neural stem cells (NSCs) are characterized by their potential for self-renewal and ability to differentiate into neurons, astrocytes, and oligodendrocytes. They are of great value to scientific studies and clinical applications. Culturing NSCs in vitro is important for characterizing their properties under controlled environmental conditions that may be modified and monitored accurately. The present study explored a modified, detailed and efficient protocol for the isolation, culture and cryo...
Source: Experimental and Therapeutic Medicine - October 24, 2020 Category: General Medicine Tags: Exp Ther Med Source Type: research

Influence of a combination of triptolide and ferulic acid on the activities of CYP450 enzymes and oxidative stress in HaCaT cells.
Authors: Zhang J, Guan Y, He L, Tao L, Zang Z, Zhu W, Chen L, Jin C Abstract Topical administration of triptolide (TP) is effective in the treatment of rheumatoid arthritis (RA), but it can also induce skin irritation. Previous studies have used data mining strategies to analyze the application of Tripterygium wilfordii in the treatment of RA and have shown that TP and ferulic acid (FA) can be used in combination due to their component compatibility. The aims of the present study were to investigate the mechanisms underlying the effects of TP treatment and to identify its effects on metabolism and oxidative damage ...
Source: Experimental and Therapeutic Medicine - October 24, 2020 Category: General Medicine Tags: Exp Ther Med Source Type: research

Clinical implication of serum CA125 for the prediction of malignancy in mucinous cystic neoplasms of the pancreas.
This study included 164 patients with MCN (low/moderate grade, 153 cases; high grade and invasive, 11 cases). The serum levels of CA125 in the high grade and invasive group (45.1±42.1 U/ml) was significantly higher than those in the low/moderate grade group (21.0±46.2 U/ml, P=0.006). The area under the receiver operating characteristic (ROC) curve of CA125 (0.75) for predicting malignancy of MCNs was higher than that of CA19-9 (0.68) or CEA (0.72). The prediction value of CA125 was improved when combined with CEA (CA125 alone, sensitivity 36.4%, specificity 90.6%, accuracy 86.6%; combined with CEA, sensitivit...
Source: Experimental and Therapeutic Medicine - October 24, 2020 Category: General Medicine Tags: Exp Ther Med Source Type: research

miR-132 improves the cognitive function of rats with Alzheimer's disease by inhibiting the MAPK1 signal pathway.
In conclusion, miR-132 inhibited hippocampal iNOS expression and oxidative stress by inhibiting MAPK1expression to improve the cognitive function of rats with AD. PMID: 33093897 [PubMed] (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - October 24, 2020 Category: General Medicine Tags: Exp Ther Med Source Type: research

Implantable collamer lens with a central hole for residual refractive error correction after corneal refractive surgery.
In conclusion, ICL V4c implantation for the correction of residual refractive error after corneal refractive surgery appeared feasible and safe and also had an excellent optical performance. However, long-term changes in visual quality require further investigation. PMID: 33093898 [PubMed] (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - October 24, 2020 Category: General Medicine Tags: Exp Ther Med Source Type: research

Moyamoya disease concurrent with dural arteriovenous fistula: A case report and literature review.
Authors: Hou K, Zhao Y, Chen X, Xu K, Yu J Abstract Moyamoya disease (MMD) and dural arteriovenous fistula (DAVF) are two distinct types of intracranial lesion that share different pathogenic mechanisms. Under rare circumstances, patients with MMD have been reported to have concurrent DAVF. The present case study reports on a 47-year-old male admitted due to sudden headache. Head CT revealed hemorrhage of the right thalamus with ventricular extension. On CT angiography, the normal vasculature in the anterior and posterior circulation disappeared and was replaced by moyamoya-like vessels. The patient received conser...
Source: Experimental and Therapeutic Medicine - October 24, 2020 Category: General Medicine Tags: Exp Ther Med Source Type: research

Safety and clinical effectiveness of intravitreal administration of bevacizumab (Lumiere ®) in patients with neovascular age-related macular degeneration.
Safety and clinical effectiveness of intravitreal administration of bevacizumab (Lumiere®) in patients with neovascular age-related macular degeneration. Exp Ther Med. 2020 Dec;20(6):162 Authors: Benisek DA, Manzitti J, Scorsetti D, Ascarza AMR, Ascarza AA, Rancaño DG, Quercia R, Gismondi MR, Total MAC, Scorsetti ML, Spitzer E, Lombas C, Deprati M, Penna MI, Fernández F, Tinelli MA Abstract The present study was an open-label, prospective, uncontrolled and multicenter clinical trial to investigate the safety and effectiveness of bevacizumab (Lumiere®) administered by the intravitr...
Source: Experimental and Therapeutic Medicine - October 24, 2020 Category: General Medicine Tags: Exp Ther Med Source Type: research

Circular RNA expression profiles significantly altered in UVA-irradiated human dermal fibroblasts.
Authors: Lin M, Zheng Y, Li Q, Liu Y, Xu Q, Li Y, Lai W Abstract Circular RNAs (circRNAs) have been previously implicated in number of diseases. However, the roles of circRNAs in photoaging remain elusive. In the present study, to understand if photoaging influences the levels of circRNA expression, the expression of circRNAs in ultraviolet A (UVA)-irradiated human dermal fibroblasts were profiled. A total of 128 circRNAs were identified to be differentially expressed (fold change>1.5; P
Source: Experimental and Therapeutic Medicine - October 24, 2020 Category: General Medicine Tags: Exp Ther Med Source Type: research

Emerging role of long non-coding RNAs in pulmonary hypertension and their molecular mechanisms (Review).
Authors: Qin Y, Yan G, Qiao Y, Wang D, Luo E, Hou J, Tang C Abstract Pulmonary hypertension (PH) is a life-threatening cardiopulmonary condition caused by several pathogenic factors. All types of PH are characterized by the excessive proliferation of pulmonary artery endothelial cells and pulmonary artery smooth muscle cells, apoptosis resistance, pulmonary vascular remodeling, sustained elevated pulmonary arterial pressure, right heart failure and even death. Over the past decade, next generation sequencing, particularly RNA-sequencing, has identified some long non-coding RNAs (lncRNAs) that may act as regulators ...
Source: Experimental and Therapeutic Medicine - October 24, 2020 Category: General Medicine Tags: Exp Ther Med Source Type: research

Impairment in locomotor activity as an objective measure of pain and analgesia in a rat model of osteoarthritis.
Authors: Alsalem M, Haddad M, Altarifi A, Aldossary SA, Kalbouneh H, Abojaradeh AM, El-Salem K Abstract A major problem with current animal models of pain is their lack of face validity and their vulnerability for false positive results. The present study evaluated the efficacy of the open field locomotor system, as an objective measure of pain-related behavior and analgesic efficacy in rodents. Adult, male, Sprague-Dawley rats (180-250 g) received intra-articular injections of monoiodoacetate (MIA; 1 mg) in the left knee joint. Mechanical allodynia using von Frey filaments, the weight bearing difference test and t...
Source: Experimental and Therapeutic Medicine - October 24, 2020 Category: General Medicine Tags: Exp Ther Med Source Type: research

Bronchogenic cyst of the stomach: A case report and literature review.
Authors: Sun B, Wang AK, Chen H, Qian BL, Yi XK, Jiang Y, Li Q, Fu WG, Li J Abstract Bronchogenic cyst (BC) is a rare congenital disease with pre-embryonic intestinal malformation. BC of the stomach is rare. The present study reported on the case of a 68-year-old male who presented with a spleen and stomach space mass detected incidentally upon a routine health examination. The patient underwent laparotomy. Postoperative histopathological diagnosis confirmed BC of the stomach. Postoperative recovery was smooth and the patient is currently under follow-up. A literature review suggested that BC is a rare disease and ...
Source: Experimental and Therapeutic Medicine - October 24, 2020 Category: General Medicine Tags: Exp Ther Med Source Type: research