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Effect of stem cell transplantation of premature ovarian failure in animal models and patients: A meta-analysis and case report.
Authors: Chen L, Guo S, Wei C, Li H, Wang H, Xu Y Abstract Stem cell transplantation has been considered a promising therapeutic approach for premature ovarian failure (POF). However, to date, no quantitative data analysis of stem cell therapy for POF has been performed. Therefore, the present study performed a meta-analysis to assess the efficacy of stem cell transplantation in improving ovarian function in animal models of POF. In addition, a case report of a patient with POF subjected to stem cell treatment was included to demonstrate that stem cell therapy also contributes to the recovery of ovarian function in...
Source: Experimental and Therapeutic Medicine - May 16, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Extracellular vesicles in mesenchymal stromal cells: A novel therapeutic strategy for stroke.
Authors: Li Y, Cheng Q, Hu G, Deng T, Wang Q, Zhou J, Su X Abstract A stroke is a focal cerebral insult that frequently causes severe neurological deficit and mortality. Recent studies have demonstrated that multipotent mesenchymal stromal cells (MSCs) hold great promise for neurovascular remodeling and neurological function recovery following a stroke. Rather than a direct replacement of parenchymal brain cells, the therapeutic mechanism of MSCs is suggested to be the secretion of soluble factors. Specifically, emerging data described MSCs as being able to release extracellular vesicles (EVs), which contain a vari...
Source: Experimental and Therapeutic Medicine - May 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Ajuga decumbens stimulates mesenchymal stem cell differentiation and regenerates cartilage in a rabbit osteoarthritis model.
Authors: Sawada Y, Sugimoto A, Osaki T, Okamoto Y Abstract In a previous study by our group, Ajuga decumbens extract (ADE) was demonstrated to decrease the number of osteoclasts in subchondral bone and to have a synergistic effect with glucosamine in improving cartilaginous injuries in a rabbit model of osteoarthritis. In the present study, a concentrate of the useful fraction of ADE, termed extra ADE (EADE), which includes higher concentrations of the active component 20-hydroxyecdysone, was evaluated for its efficacy to accelerate the healing of experimental cartilage injury. Cartilage injuries were surgically in...
Source: Experimental and Therapeutic Medicine - May 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Predictors of decreased left ventricular function subsequent to follow-up echocardiography after percutaneous coronary intervention following acute ST-elevation myocardial infarction.
Authors: Kim DH, Park CB, Jin ES, Hwang HJ, Sohn IS, Cho JM, Kim CJ Abstract The preferred treatment for patients with ST elevation myocardial infarction (STEMI) is primary percutaneous coronary intervention (PCI). However, not all patients improve or maintain heart function following primary PCI, and certain patients may experience decreased heart function. The present study investigated factors associated with left ventricular (LV) dysfunction, and improvement or deterioration of LV ejection fraction (LVEF) at follow-up echocardiography following successful primary PCI. The clinical outcomes following primary PCI...
Source: Experimental and Therapeutic Medicine - May 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Resveratrol attenuates neurological deficit and neuroinflammation following intracerebral hemorrhage.
Authors: Cai JC, Liu W, Lu F, Kong WB, Zhou XX, Miao P, Lei CX, Wang Y Abstract Resveratrol (RESV) improves histopathological and behavioral outcomes in diseases of the central nervous system. However, to the best of our knowledge, there have been no studies investigating its neuroprotective effects on secondary brain injury following intracerebral hemorrhage (ICH). The aim of the present study was to evaluate the neuroprotective function of resveratrol following ICH. Male Sprague-Dawley rats were randomly divided into 3 groups: Sham, ICH and ICH+RESV groups. Rats underwent ICH and received an intraperitoneal injec...
Source: Experimental and Therapeutic Medicine - May 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Overexpression of Csk-binding protein/phosphoprotein associated with glycosphingolipid-enriched microdomains induces cluster of differentiation 59-mediated apoptosis in Jurkat cells.
Authors: Cong BB, Gao MH, Li B, Wang B, Zhang B, Wang LN, Zhang SC, Li HQ, Wang Z, Han SY Abstract Csk-binding protein/phosphoprotein associated with glycosphingolipid-enriched microdomains (CBP/PAG) is a membrane-bound adaptor protein that downregulates the activation of Src family kinases present in lipid rafts. To elucidate the role of CBP/PAG in human T cell activation, a cell line overexpressing CBP/PAG was constructed and the function of CBP/PAG in Jurkat cells was examined. The present study revealed that increased CBP/PAG expression in T cells significantly enhanced their apoptosis and reduced cellular acti...
Source: Experimental and Therapeutic Medicine - May 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

ASXL1 mutations in Chinese patients with essential thrombocythemia.
In conclusion, ASXL1-mutant ET patients are prone to experiencing thrombotic events. There was no significant difference in the occurrence of thrombotic events among CARL-mutant, JAK2 V617F-mutant and TN patients. Furthermore, ASXL1-mutant/TN patients exhibited a higher number of PLT than ASXL1/JAK2 V617F-double mutant patients. Therefore, ASXL1 mutations may be a risk factor for the occurrence of thrombotic events in ET patients. PMID: 29725364 [PubMed] (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - May 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Effects of recombinant human growth hormone on protein malnutrition and IGF-1 and IL-2 gene expression levels in chronic nephrotic syndrome.
Authors: Dai G, Wang D, Dong H Abstract The aim of the study was to investigate the effects of recombinant human growth hormone on protein malnutrition and insulin-like growth factor-1 (IGF-1) and interleukin-2 (IL-2) gene expressions in chronic nephrotic syndrome. Eighty patients with chronic nephrotic syndrome were admitted to our hospital. The patients were included in the study period from January 2015 to December 2016 and were divided into two groups (40 cases in each group) according to the random number method. All the patients enrolled received symptomatic and supportive treatment. The observation group was...
Source: Experimental and Therapeutic Medicine - May 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Application values of miR-194 and miR-29 in the diagnosis and prognosis of gastric cancer.
Authors: Zhao X, Hou Y, Tuo Z, Wei F Abstract The object of this study was to analyze the expression of miR-194 and miR-29 in gastric cancer and their roles in the regulation of malignant phenotype of gastric cancer cells, and to explore the application value of miR-194 and miR-29 in diagnosis and prognosis of gastric cancer. Tumor tissue and adjacent healthy tissue of 165 gastric cancer patients diagnosed by pathologic examinations were collected. Expression of miR-194 and miR-29 in the tissues was detected by RT-PCR. The relationship between miR-194 and miR-29 expression and clinical data was analyzed. SGC7901 ce...
Source: Experimental and Therapeutic Medicine - May 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Diaphragmatic smears are not of additional benefit in the detection of peritoneal spread in gynecological cancers.
Authors: Montavon C, Mirza U, Fedier A, Schoetzau A, Zanetti Dällenbach R, Heinzelmann-Schwarz V Abstract Peritoneal biopsies (PB) and peritoneal washing (PW) are routine measures in abdominal staging of gynecological malignancies and are used particularly for the assessment of occult microscopic tumor spread to the peritoneal surface including the diaphragm. Cytological diaphragmatic smears (DS) have been suggested as a supplemental tool; however, they are not routinely taken and their usefulness is still unclear. The present study retrospectively evaluated whether DS provide an additional benefit over PB and...
Source: Experimental and Therapeutic Medicine - May 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

CCAAT/enhancer binding protein homologous protein knockdown alleviates hypoxia-induced myocardial injury in rat cardiomyocytes exposed to high glucose.
Authors: Yang W, Wu F, Luo T, Zhang Y Abstract Diabetic patients are more sensitive to ischemic injury than non-diabetics. Endoplasmic reticulum (ER) stress has been reported to be closely associated with the pathophysiology of ischemic injury in diabetes. The aim of the present study was to investigate the mechanisms involved in the progression of diabetes complicated by myocardial infarction (MI) and further verify the role of CCAAT/enhancer binding protein (C/EBP)-homologous protein (CHOP) using an in vitro model of diabetes/MI. The rats were exposed to 65 mg/kg streptozotocin (STZ) and left anterior descending ...
Source: Experimental and Therapeutic Medicine - May 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Ethyl pyruvate can alleviate alcoholic liver disease through inhibiting Nrf2 signaling pathway.
Authors: Shen F, Wang Z, Liu W, Liang Y Abstract The effects of ethyl pyruvate (EP) on alcoholic liver disease and its related mechanism were investigated. Thirty male C57/BL6 mice were randomly divided to three groups: Control (n=10), alcoholic liver disease (ALD, n=10) and ethyl pyruvate group (EP, n=10). EP group was treated with gavage using EP (100 mg/kg) for 15 consecutive days. Control and ALD group were treated with the same volume of normal saline. After the last gavage, EP and ALD group were treated with the intraperitoneal injection of 50% alcoholic solution (10 ml/kg). After that, ALD and EP group recei...
Source: Experimental and Therapeutic Medicine - May 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Upregulation of c-mesenchymal epithelial transition expression and RAS mutations are associated with late lung metastasis and poor prognosis in colorectal carcinoma.
Authors: Liu J, Zeng W, Huang C, Wang J, Xu L, Ma D Abstract The present study aimed to investigate whether c-mesenchymal epithelial transition factor (C-MET) overexpression combined with RAS (including KRAS, NRAS and HRAS) or BRAF mutations were associated with late distant metastases and the prognosis of patients with colorectal cancer (CRC). A total of 374 patients with stage III CRC were classified into 4 groups based on RAS/BRAF and C-MET status for comprehensive analysis. Mutations in RAS/BRAF were determined using Sanger sequencing and C-MET expression was examined using immunohistochemistry. The association...
Source: Experimental and Therapeutic Medicine - May 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Analyses of risk factors for polycystic ovary syndrome complicated with non-alcoholic fatty liver disease.
Authors: Zhang J, Hu J, Zhang C, Jiao Y, Kong X, Wang W Abstract The risk factors related to polycystic ovary syndrome (PCOS) patients complicated with non-alcoholic fatty liver disease (NAFLD) were investigated. A total of 188 PCOS patients treated in Shengli Oilfield Central Hospital (Dongying, China) from February 2014 to February 2015 were retrospectively analyzed as PCOS group, and PCOS group was further divided into NAFLD group and non-NAFLD (N-NAFLD) group according to the liver B ultrasound. In the same time-period, 65 healthy people were selected as normal control group. The differences of clinical, bioche...
Source: Experimental and Therapeutic Medicine - May 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Identification of long non-coding RNA and mRNA expression in βΒ2-crystallin knockout mice.
Identification of long non-coding RNA and mRNA expression in βΒ2-crystallin knockout mice. Exp Ther Med. 2018 May;15(5):4277-4283 Authors: Jia Y, Xiong K, Ren HX, Li WJ Abstract βΒ2-crystallin (CRYBB2) is expressed at an increased level in the postnatal lens cortex and is associated with cataracts. Improved understanding of the underlying biology of cataracts is likely to be critical for the development of early detection strategies and new therapeutics. The present study aimed to identify long non-coding RNAs (lncRNAs) and mRNAs associated with CRYBB2 knockdown (KO)-induced catarac...
Source: Experimental and Therapeutic Medicine - May 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Evaluation of circulating placenta-related long noncoding RNAs as potential biomarkers for preeclampsia.
Authors: Wang X, Chen Y, Du L, Li X, Li X, Chen D Abstract Increasing evidence has indicated that circulating placental RNAs may reflect the pathophysiology of the placenta. In the current study, circulating placenta-related long noncoding RNAs (lncRNAs) were evaluated as potential biomarkers for preeclampsia (PE). Two parts of the case-control study were simultaneously executed, including the following sets: 52 patients with late-onset PE (LOPE) (diagnosed after 34 weeks) and 52 gestational age (GA)-matched controls; 58 patients with early-onset PE (EOPE) (diagnosed before 34 weeks) and 58 GA-matched controls. Lnc...
Source: Experimental and Therapeutic Medicine - May 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Decreased expression of microRNA-223 promotes cell proliferation in hepatocellular carcinoma cells via the insulin-like growth factor-1 signaling pathway.
Authors: Zhang C, Zhang J Abstract Hepatocellular carcinoma (HCC) is one of the most harmful types of cancer. Previous studies have demonstrated that microRNA (miR)-223 is downregulated in the serum and tumor tissue of patients with HCC. The present study aimed to investigate the regulatory role of miR-223 on insulin-like growth factor-1 receptor (IGF-1R) and downstream factors in HCC. The Hep3B cell line was transfected with miR-223 mimic and inhibitor. Following transfection, cell proliferation was analyzed using a cell counting kit 8 assay and cellular apoptosis was assessed using flow cytometry. The expression ...
Source: Experimental and Therapeutic Medicine - May 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Expression of SATB1 and E-cad in tissues of patients with endometrial carcinoma and the relationship with clinicopathological features.
Authors: Feng Y, Wang X, Wang Q Abstract The expression of special AT-rich sequence binding protein 1 (SATB1) and E-cadherin (E-cad) in tissues of patients with endometrial carcinoma and the relationships with clinicopathological features were investigated. One hundred and four cases of carcinoma tissues and 104 cases of para-carcinoma tissues of patients pathologically diagnosed as endometrial carcinoma in Affiliated Hospital of Jining Medical University (Jining, China) from August 2015 to August 2016 were selected. The expressions of SATB1 and E-cad in tissues was detected via streptavidin peroxidase biotin (SP) ...
Source: Experimental and Therapeutic Medicine - May 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Low-dose aspirin at ≤16 weeks of gestation for preventing preeclampsia and its maternal and neonatal adverse outcomes: A systematic review and meta-analysis.
Low-dose aspirin at ≤16 weeks of gestation for preventing preeclampsia and its maternal and neonatal adverse outcomes: A systematic review and meta-analysis. Exp Ther Med. 2018 May;15(5):4361-4369 Authors: Cui Y, Zhu B, Zheng F Abstract The aim of the present meta-analysis study was to evaluate the efficacy of low-dose aspirin, commenced at ≤16 weeks of gestation, in preventing preterm and term preeclampsia, as well as associated maternal and neonatal adverse events in women at risk of preeclampsia. The Embase, PubMed, Cochrane Central Register of Controlled Trials and the Web of Science database...
Source: Experimental and Therapeutic Medicine - May 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

The siRNA silencing of DcR3 expression induces Fas ligand-mediated apoptosis in HepG2 cells.
Authors: Zhao T, Xu Y, Ren S, Liang C, Zhou X, Wu J Abstract Dysfunctional Fas ligand (FasL) may inhibit the apoptosis of tumor cells. FasL contains two receptors, Fas and Decoy Receptor 3 (DcR3). DcR3 competitively binds to FasL over Fas, resulting in the inhibition of FasL-mediated apoptosis. Therefore, it was suggested that the downregulation of DcR3 expression enhances FasL-mediated apoptosis. In the current study, the expression of DcR3 was silenced in liver cancer HepG2 cells in order to study the effect of FasL on HepG2 cell activity and invasiveness. DcR3 siRNA knockdown HepG2 cells (KD), DcR3 blank plasmid...
Source: Experimental and Therapeutic Medicine - May 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Homocysteine induces oxidative stress to damage trabecular meshwork cells.
Authors: You ZP, Zhang YZ, Zhang YL, Shi L, Shi K Abstract The aim of the present study was to investigate the effect of homocysteine (Hcy) in on human trabecular meshwork cells (HTMCs). A total of 41 patients with primary open-angle glaucoma (POAG) and 53 patients with senile cataracts (control group) were recruited. Plasma and aqueous humor samples were collected and the Hcy concentrations were determined using enzymatic cycling assays. In cell experiments, normal HTMCs were passaged and randomly divided into a blank control group, a normal HTMC group and experimental groups, which were treated with different con...
Source: Experimental and Therapeutic Medicine - May 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Evaluation of effect of atorvastatin on left ventricular systolic function in rats with myocardial infarction via 2D-STI technique.
This report aims to evaluate the effect of atorvastatin (Ator) on left ventricular systolic function in myocardial infarction (MI) rats. Forty healthy adult Sprague-Dawley rats were randomly divided into four groups: Ator group, MI group, sham-operation group and normal group. The left anterior descending coronary arteries were ligated to establish the MI model; after modeling, the Ator group was treated with Ator for 4 consecutive weeks. The echocardiographic detection was performed; the left ventricular myocardial systolic peak velocities, strain and strain rates were analyzed using the 2D-STI technique. After 4 weeks, m...
Source: Experimental and Therapeutic Medicine - May 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

TGF- β3 reduces apoptosis in ischemia-induced adipose-derived stem cells by enhancing DNA repair.
TGF-β3 reduces apoptosis in ischemia-induced adipose-derived stem cells by enhancing DNA repair. Exp Ther Med. 2018 May;15(5):4400-4408 Authors: Wu F, Ye H, Lin J, Xu Y, Zhang Z, Xiong H, Laing M, Zhen Y, Chen S Abstract Adipose-derived stem cells (ADSCs) possess good proliferative and differentiative abilities, making then a promising candidate for the treatment of cartilage defects. However, local ischemia often causes apoptosis in ADSCs. Transforming growth factor-β3 (TGF-β3) is often used as a chondrogenic differentiation cytokine whose function in apoptosis is unclear. The aim of th...
Source: Experimental and Therapeutic Medicine - May 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Soleus muscle H-reflex monitoring in endoscopic surgery under general anesthesia percutaneous interlaminar approach.
Authors: Wang H, Gao Y, Ji L, Bai W Abstract The clinical value of soleus muscle H-reflex monitoring in general anesthesia percutaneous interlaminar approach was investigated. A total of 80 cases with unilateral L5-S1 disc herniation between January 2015 and October 2016 were randomly divided into control group (without soleus muscle H-reflex monitoring, n=40) and observation group (with soleus muscle H-reflex monitoring, n=40). Results showed that the operation time of the observation group was shorter than that of the control group (P
Source: Experimental and Therapeutic Medicine - May 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Effects of cisatracurium in combination with ventilation on inflammatory factors and immune variations in sepsis rats.
Authors: He T, Tao J, Wang X, Wang X Abstract The effects of cisatracurium in combination with ventilation on inflammatory factors and immune variations in sepsis rats were investigated. A total of 54 male Sprague-Dawley rats were selected and divided randomly into three groups: Sham group (n=6), model group (n=24) and experiment group (n=24). Rats in the model and experiment groups underwent cecal ligation and puncture (CLP) for establishment of sepsis model. Rats in experiment group additionally received cisatracurium medication in combination with ventilation for treatment. At 6, 12 and 24 h after CLP, the level...
Source: Experimental and Therapeutic Medicine - May 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Therapeutic effect of radiofrequency ablation on children with supraventricular tachycardia and the risk factors for postoperative recurrence.
Authors: Li C, Jia L, Wang Z, Niu L, An X Abstract The present study investigated the therapeutic effect of radiofrequency ablation on children with supraventricular tachycardia (SVT), and explored the risk factors for postoperative recurrence. A total of 312 patients with pediatric SVT were selected in the Affiliated Children's Hospital of Xuzhou Medical University from April, 2011 to March, 2017. All the patients were subjected to radiofrequency ablation, and clinical data were retrospectively analyzed. Tilt table test was performed before and after treatment, and heart rate, systolic and diastolic blood pressure...
Source: Experimental and Therapeutic Medicine - May 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Improved plaque neovascularization following 2-year atorvastatin therapy based on contrast-enhanced ultrasonography: A pilot study.
Authors: Xu B, Xing J, Wu W, Zhang WJ, Zhu QQ, Zhang D, Sun NN, Wu C, Kang GJ, Zhai L, Li WD, Meng Y, Du TY Abstract The present study assessed changes in carotid plaque neovascularization following long-term atorvastatin therapy (20 mg/day) using contrast-enhanced ultrasonography (CEUS). In this prospective case series, seven males (mean age, 68±9 years) and three females (mean age, 67±10 years) with a total of 13 carotid plaques underwent standard ultrasonography and CEUS at baseline, as well as after 1 and 2 years of atorvastatin treatment. The same plaques were then examined using real-time CEUS. ...
Source: Experimental and Therapeutic Medicine - May 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Atypical synovial chondromatosis of the right knee: A case report.
Authors: Yang YP, Wang JJ, Li HY Abstract Synovial chondromatosis, also known as synovial osteochondromatosis, is a rare, benign condition characterized by the formation of multiple cartilaginous nodules in the synovium of facet spaces. Synovial chondromatosis affects many joints, the knee being the most common. The present report examined a 47-year-old male with symptoms of swelling and pain in the right knee, who was admitted to hospital in September 2015. Following admittance, arthroscopic explorations were conducted. Viscous fluid and multiple cartilage-like clumps were identified in the patient's joints during...
Source: Experimental and Therapeutic Medicine - May 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Downregulation of microRNA-193-3p inhibits the progression of intrahepatic cholangiocarcinoma cells by upregulating TGFBR3.
Authors: Han YL, Yin JJ, Cong JJ Abstract The aim of this study was to investigate the function of microRNA (miR)-193-3p in human intrahepatic cholangiocarcinoma (ICC) tissues and cells. To evaluate whether miR-193-3p was aberrantly upregulated, we used reverse transcription-quantitative polymerase chain reaction to detect the level of miR-193-3p in ICC tissues and ICC-9810 cells. The effects of miR-193-3p downregulation on ICC cell proliferation, migration and invasion were also measured by MTT, wound-healing and Transwell assays. Additionally, transforming growth factor-β receptor type 3 (TGFBR3) was investi...
Source: Experimental and Therapeutic Medicine - May 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

PF-2341066 combined with celecoxib promotes apoptosis and inhibits proliferation in human cholangiocarcinoma QBC939 cells.
Authors: Chen C, Yang D, Zeng Q, Luo L, Cai C Abstract Intrahepatic cholangiocarcinoma (ICC) is a malignant tumor with high incidence and an average age of onset of 50-70 years old. However, at present there is no effective treatment for this disease. The aim of the present study was to investigate the effects of a c-Met inhibitor, PF-2341066 and a cyclooxygenase-2 (COX-2) inhibitor, celecoxib, on c-Met and COX-2 expression, proliferation and apoptosis. The results demonstrated that c-Met and COX-2 are highly expressed in hepatobiliary calculus with cholangiocarcinoma. PF-2341066 was able to downregulate the expres...
Source: Experimental and Therapeutic Medicine - May 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

MicroRNA-381 regulates the occurrence and immune responses of coronary atherosclerosis via cyclooxygenase-2.
Authors: Song K, Li L, Sun G, Wei Y Abstract The present study aimed to measure the levels of microRNA-381 (miR-381) in the plaque tissues, peripheral blood mononuclear cells (PBMCs) and serum of patients with coronary atherosclerosis. In addition, the regulatory mechanisms of miR-381 and cyclooxygenase (COX)-2 in coronary atherosclerosis were investigated. A total of 36 patients with coronary atherosclerosis who received coronary endarterectomy at Linyi People's Hospital and Junan Hospital of Traditional Chinese Medicine (Linyi, China) between January 2013 and June 2016 were enrolled into the present study, while ...
Source: Experimental and Therapeutic Medicine - May 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Imaging characteristics and pathogenesis of intracranial artery stenosis in patients with acute cerebral infarction.
Authors: Xu W, Xie N, Zhang C, Huang Q Abstract The current study aimed to investigate the imaging characteristics and pathogenesis of intracranial artery stenosis in patients with acute cerebral infarction. In total, 84 patients diagnosed with acute cerebral infarction were recruited. Magnetic resonance angiography was performed to detect the existence of intracranial artery stenosis or occlusion. In addition, magnetic resonance imaging and diffusion weighted imaging were employed to analyze the infarction types and characteristics. In the majority of patients, the infarction resulted from internal carotid stenosi...
Source: Experimental and Therapeutic Medicine - May 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Effect of gastric bypass combined with ileal transportation on type 2 diabetes mellitus.
Authors: Gao Z, Wang B, Gong X, Yao C, Ren D, Shao L, Pang Y, Liu J Abstract Type 2 diabetes mellitus (T2DM) is a chronic progressive disease, which manifests as an endocrine disorder. Among the different methods of surgery available to treat patients with T2DM, Roux-en-Y gastric bypass (RYGBP) and ileal transposition (IT) are the most commonly performed. The aim of the present study was to investigate the effects of RYGBP combined with IT on rats with T2DM. A total of 8 healthy male rats were used as a control group and 40 GK rats were randomly divided into 5 groups: A diabetes mellitus (DM) group, a sham operativ...
Source: Experimental and Therapeutic Medicine - May 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

2D-STI combined with gated 99Tcm-MIBI MPI for the diagnosis of myocardial ischemia in hypercholesterolemia patients.
Authors: Song Y, Zhang RF, Liu Y Abstract [This retracts the article DOI: 10.3892/etm.2017.4602.]. PMID: 29725391 [PubMed - in process] (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - May 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Sevoflurane-induced memory impairment in the postnatal developing mouse brain.
Authors: Lu Z, Sun J, Xin Y, Chen K, Ding W, Wang Y Abstract The aim of the present study was to confirm that sevoflurane induces memory impairment in the postnatal developing mouse brain and determine its mechanism of action. C57BL/6 mice 7 days old were randomly assigned into a 2.6% sevoflurane (n=68), a 1.3% sevoflurane (n=68) and a control (n=38) group. Blood gas analysis was performed to evaluate hypoxia and respiratory depression during anesthesia in 78 mice. Measurements for expression of caspase-3 by immunohistochemistry, cleavage of poly adenosine diphosphate-ribose polymerase (PARP) by western blotting, a...
Source: Experimental and Therapeutic Medicine - May 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Morphology of the lumbar multifidus muscle in lumbar disc herniation at different durations and at different ages.
In conclusion, the reduced rate of relative functional net LM muscle volume was positively correlated with age, duration, VAS scores and JOA scores. Although these findings are unreliable markers of lumbar pathology in an individual level for use in clinical or research settings, there is a notable importance of early intervention in LM muscle atrophy. PMID: 29731814 [PubMed] (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - May 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Oral lichen planus and malignant transformation: The role of p16, Ki-67, Bub-3 and SOX4 in assessing precancerous potential.
Authors: Rosa EA, Hurtado-Puerto AM, Falcão DP, Brietzke AP, De Almeida Prado Franceschi LE, Cavalcanti Neto FF, Tiziane V, Carneiro FP, Kogawa EM, Moreno H, Amorim RFB Abstract The association of oral lichen planus (OLP) lesions with malignant transformation risk has remained a controversial topic and is of clinical importance. Therefore, the present study evaluated the expression levels of p16, Ki-67, budding uninhibited by benzimidazoles 3 (Bub-3) and sex-determining region Y-related high mobility group box 4 (SOX4), and their roles as precancerous biomarkers in OLP. A retrospective study was performed, i...
Source: Experimental and Therapeutic Medicine - May 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Pressure ulcer healing promoted by adequate protein intake in rats.
Authors: Qin Z, Wang Y, Zhao W, Zhang Y, Tian Y, Sun S, Li X Abstract The effect of protein intake on rat pressure ulcer healing was evaluated. One hundred rats were numbered according to body weight and then they were randomly divided into 4 groups (n=25) using the random number table. After rat models of stage II pressure ulcer were established, they were fed with feed containing different protein levels (10, 15, 20 and 25%). Healing time, pressure ulcer area, body weight, albumin (ALB) and hemoglobin (Hb) levels among groups were compared. Hematoxylin and eosin (H&E) staining was also performed to observe pr...
Source: Experimental and Therapeutic Medicine - May 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Leptin attenuates the growth of rabbit mesenchymal stem cells via the extracellular signal-regulated kinase signaling pathway.
In conclusion, the results of the present study demonstrated that leptin inhibits the growth of rBMSCs principally via the ERK1/2 signaling pathway. PMID: 29731817 [PubMed] (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - May 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Methotrexate remediates spinal cord injury in vivo and in vitro via suppression of endoplasmic reticulum stress-induced apoptosis.
Authors: Rong F, Gao X, Liu K, Wu J Abstract It has been suggested that endoplasmic reticulum stress (ERS) may induce apoptosis following spinal cord injury (SCI). Methotrexate (MTX) has been used as a long-term therapy regimen for rheumatoid arthritis. However, it is not clear whether MTX remediates SCI by inhibiting ERS. In the present study, to establish an in vitro ERS cell model, PC12 cells were pre-incubated with triglycerides (TG). MTT assays revealed that treatment with 1, 2.5, 5 and 10 µM TG decreased PC12 cell viability in a dose-dependent manner. Additionally, MTX treatment significantly reversed t...
Source: Experimental and Therapeutic Medicine - May 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Cytoprotective effect of aquaporin 1 against lipopolysaccharide-induced apoptosis and inflammation of renal epithelial HK-2 cells.
Authors: Wang Y, Zhang W, Yu G, Liu Q, Jin Y Abstract Sepsis is the most common underlying disease of disseminated intravascular coagulation. Acute kidney injury is a common and serious complications of sepsis. In the present study, a lipopolysaccharide (LPS)-induced human proximal tubule cell line (HK-2 cells) was selected as an in vitro model of septic acute kidney injury. The aim of the present study was to investigate whether aquaporin 1 (AQP-1) has a cytoprotective role in LPS-induced HK-2 cells. HK-2 cells were treated with 0-16 µg/ml LPS for 0-24 h to establish the in vitro model of sepsis. The results...
Source: Experimental and Therapeutic Medicine - May 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Multimodal monitoring combined with hypothermia for the management of severe traumatic brain injury: A case report.
Authors: Chen JH, Xu YN, Ji M, Li PP, Yang LK, Wang YH Abstract Traumatic brain injury (TBI) is a prominent public health issue that has a significant negative impact on patients and their family members. It is the leading cause of mortality and disability among young (below 50 years old) individuals. Intracranial hypertension (ICH) remains the single most difficult therapeutic challenge for the management of severe TBI. Therapeutic hypothermia may reduce intracranial hypertension and improve patient outcomes; however, the use of hypothermia is controversial. It has been reported that therapeutic hypothermia elicit...
Source: Experimental and Therapeutic Medicine - May 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Clinical significance of the detection of procalcitonin and C-reactive protein in the intensive care unit.
Authors: Li Q, Gong X Abstract The identification significance of C-reactive protein (CRP) and procalcitonin (PCT) levels in the intensive care unit patients with combined infection and their prognostic effects of patients with sepsis was investigated. A total of 203 patients were divided into the sepsis (n=60) and the non-sepsis group (n=143). The predictive effects of CRP and PCT levels in patients in the intensive care unit on sepsis and their effects on the prognosis of patients with sepsis were analyzed. The results showed that CRP and PCT levels in patients in the sepsis were higher than those in the non-seps...
Source: Experimental and Therapeutic Medicine - May 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

CBX3/HP1 γ is upregulated in tongue squamous cell carcinoma and is associated with an unfavorable prognosis.
CBX3/HP1γ is upregulated in tongue squamous cell carcinoma and is associated with an unfavorable prognosis. Exp Ther Med. 2018 May;15(5):4271-4276 Authors: Zhang H, Fu X, Su X, Yang A Abstract Increased expression of CBX3/HP1γ, a core component of heterochromatin protein 1, has recently proved to be involved in human tumorigenesis and patient prognosis. The present study aimed to investigate the expression of CBX3/HP1γ and its clinicopathological significance in primary tongue squamous cell carcinoma (TSCC). Gene expression profiles of CBX3/HP1γ in TSCC from Oncomine database we...
Source: Experimental and Therapeutic Medicine - May 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

(-)-Epigallocatechin-3-gallate protects PC12 cells against corticosterone-induced neurotoxicity via the hedgehog signaling pathway.
Authors: Feng S, Liu J, Cheng B, Deng A, Zhang H Abstract It has been acknowledged that environmental stress is a risk factor for developing mental disorders. Chronic stress may contribute to the hyperactivation of the hypothalamic-pituitary-adrenal (HPA) axis and a sustained rise in the levels of glucocorticoids (GCs). A high concentration of corticosterone (CORT) damages neuronal PC12 cells. It has been reported that (-)-Epigallocatechin-3-gallate (EGCG), a major component of green tea, exhibits neuroprotective activity. However, the protective effect of EGCG on neuronal cells injured by CORT remains to be elucid...
Source: Experimental and Therapeutic Medicine - May 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

MicroRNA-26a inhibits hyperplastic scar formation by targeting Smad2.
In conclusion, the results of the present study indicate that miR-26a inhibits HS formation by modulating proliferation and apoptosis ad well as inhibiting the expression of extracellular matrix-associated proteins by targeting Smad2. PMID: 29731824 [PubMed] (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - May 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

MicroRNA-27a participates in the pathological process of depression in rats by regulating VEGFA.
Authors: Cui J, Gong C, Cao B, Li L Abstract The present study aimed to determine the expression of vascular endothelial growth factor A (VEGFA) and microRNA (miRNA/miR)-27a in hippocampal tissues, and serum from a depression model of rats. In addition, the present study aimed to understand the mechanism of regulation of miR-27a in depression. A total of 40 male rats were selected, and divided into the control and depression model groups. The rats in the model group were subjected to 14 types of stimulations to model depression. By determining the body weight, syrup consumption rate and open field test score, the e...
Source: Experimental and Therapeutic Medicine - May 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Expression and influence of pentraxin-3, HbAlc and ApoA1/ApoB in serum of patients with acute myocardial infarction combined with diabetes mellitus type 2.
Authors: Zhu Y, Wang X, Wang W, Wang H, Zhang F Abstract The present study investigated the clinical significance of changes in levels of hypersensitive plasma pentraxin-3 (PTX3), hemoglobin A1c (HbAlc) and apolipoprotein A-I (ApoA1)/apolipoprotein B (ApoB) on patients with acute myocardial infarction (AMI) and diabetes mellitus type 2 (T2DM). After admission, 100 patients diagnosed with AMI combined with T2DM were selected as Group A. According to the level of fasting blood glucose (FBG), they were then subdivided into Group A1 (n=44) with FBG ≥13.0 mmol/l and Group A2 (n=56) with FBG
Source: Experimental and Therapeutic Medicine - May 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Leptin, hs-CRP, IL-18 and urinary protein before and after treatment of children with nephrotic syndrome.
In conclusion, the detection of changes in plasma leptin, serum inflammatory factors (hs-CRP and IL-18) and urinary protein in children with nephrotic syndrome before and after treatment has important reference value for reflecting the severity of disease and evaluating the prognosis of children with nephrotic syndrome. PMID: 29731827 [PubMed] (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - May 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Puerarin alleviates delayed-type hypersensitivity via cytokine inhibition by modulating Th1/Th2 balance.
Authors: Tong J, Hu XJ, Cai WQ, Dai X, Wang L Abstract Puerarin has long been used as a traditional Chinese medicine, which possesses various physiological properties, including anti-inflammation, anti-oxidative, anti-diabetic and anti-cancer activities. The aim of the current study was to investigate the effect of puerarin on delayed-type hypersensitivity (DTH) induced by ovalbumin (OVA) in mice and explore its underlying mechanisms. The results showed that puerarin significantly attenuated DTH, resulting from a decrement in footpad swelling, reduction in inflammatory cell as well as a decline in anti-OVA IgG in s...
Source: Experimental and Therapeutic Medicine - May 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research