Expression of somatostatin and its receptor 1-5 in endometriotic tissues and cells.
In conclusion, SS was highly expressed in EECs. SSTR1-5 were expressed in the ectopic as well as eutopic endometrium, and low or moderate expression of SSTR1-4 and high expression of SSTR5 were detected in the ectopic and eutopic endometrial tissues, while low expression of SSTR1-4 and partial expression of SSTR5 were detected in normal endometrium. The positive rates of expression of SSTR1-5 in the EE cells and eutopic endometrium were higher than those in the normal endometrium. The expression of all the subtypes of SSTR in the EE tissues was closely associated with EMS staging. PMID: 30405748 [PubMed] (Source: Exper...
Source: Experimental and Therapeutic Medicine - November 10, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

How pH is regulated during amelogenesis in dental fluorosis.
Authors: Ji M, Xiao L, Xu L, Huang S, Zhang D Abstract Amelogenesis is a complicated process that concerns the interaction between growing hydroxyapatite crystals and extracellular proteins, which requires the tight regulation of pH. In dental fluorosis, the balance of pH regulation is broken, leading to abnormal mineralization. The current review focuses on the electrolyte transport processes associated with pH homeostasis, particularly regarding the changes in ion transporters that occur during amelogenesis, following exposure to excessive fluoride. Furthermore, the possible mechanism of fluorosis is discussed on...
Source: Experimental and Therapeutic Medicine - November 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

High FNDC1 expression correlates with poor prognosis in gastric cancer.
The objectives of the present study were to measure FNDC1 expression in gastric cancer tissues and evaluate its value as a potential prognostic marker for gastric cancer. FNDC1 protein expression was analyzed by immunohistochemistry in 98 samples of gastric cancer tissue and 25 adjacent normal tissues. The associations between FNDC1 level and various clinicopathological characteristics were assessed, and the correlation between FNDC1 expression levels and prognosis of patients with gastric cancer was analyzed using a Kaplan-Meier analysis. It was demonstrated that FNDC1 expression in gastric cancer tissues and adjacent tis...
Source: Experimental and Therapeutic Medicine - November 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Efficacy of second-look endoscopy in preventing delayed bleeding after endoscopic submucosal dissection of early gastric cancer.
Authors: Guo Z, Miao L, Chen L, Hao H, Xin Y Abstract The present study aimed to evaluate whether second-look endoscopy (SLE) is able to prevent delayed bleeding after endoscopic submucosal dissection (ESD) of gastric carcinoma and to identify which types of lesion require SLE. ESD of gastric cancer at the early stage was performed on 210 patients between October 2014 and September 2016. Mucosal damage-associated bleeding within 24 h after ESD was considered as delayed bleeding. The association of the characteristics of patients and lesions, as well as surgical factors, with the incidence of bleeding as a measure o...
Source: Experimental and Therapeutic Medicine - November 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

A study on the prevention and treatment of murine calvarial inflammatory osteolysis induced by ultra-high-molecular-weight polyethylene particles with neomangiferin.
Authors: Wang HT, Li J, Ma ST, Feng WY, Wang Q, Zhou HY, Zhao JM, Yao J Abstract The present study aimed to examine the influence of neomangiferin on murine calvarial inflammatory osteolysis induced by ultra-high-molecular-weight polyethylene (UHMWPE) particles. Eight-week-old male C57BL/J6 mice served as an inflammatory osteolysis model, in which UHMWPE particles were implanted into the calvarial subperiosteal space. The mice were randomly distributed into four groups and treated with different interventions; namely, a sham group [phosphate-buffered saline (PBS) injection and no UHMWPE particles], model group (PBS...
Source: Experimental and Therapeutic Medicine - November 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Improved expression of recombinant fusion defensin gene plasmids packed with chitosan-derived nanoparticles and effect on antibacteria and mouse immunity.
Authors: Wan X, Chen J, Cheng C, Zhang H, Zhao S, Li J, Lv X, Wang Z, Gao R Abstract In order to develop a secure and competent technique to express the human immune gene for fighting infections, we cloned and expressed the BD2/3 using VR1020 (a eukaryotic expression plasmid). BD2/3 contains human β-defensin 2 (BD2) and human BD3. To explore safe and effective DNA delivery molecules in vitro and in vivo, the fusion genes of BD2/3 were used as an immune-labelled gene to verify transfection effectivness of modified chitosan (CS). Plasmid of VR1020-BD2/3 was packed with biomaterials: CS, average molecular weight:...
Source: Experimental and Therapeutic Medicine - November 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Role of miR-520b in non-small cell lung cancer.
Authors: Zhang L, Yu S Abstract The aim of the present study was to investigate the expression of microRNA (miR)-520b in non-small cell lung cancer (NSCLC) and its biological functions. Reverse transcription-quantitative polymerase chain reaction was used to detect the expression of miR-520b in 52 cases of NSCLC tissues, and its associations with tumor clinical staging and lymph node metastasis were analyzed. miR-520b mimics was transfected into A549 and Calu-3 cells. Cell proliferation, cell cycle, and cell invasion and migration abilities were assessed via cell counting kit-8 assay, flow cytometry and Transwell c...
Source: Experimental and Therapeutic Medicine - November 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Enhancement of osteogenesis of rabbit bone marrow derived mesenchymal stem cells by transfection of human BMP-2 and EGFP recombinant adenovirus via Wnt signaling pathway.
Authors: Wu CC, Wang F, Rong S, Ren J, Wan JS, Shi LX, Wu Z, Liu T, Li Q Abstract Bone marrow mesenchymal stem cells (BMSCs) are considered the most important seed cells in bone tissue engineering. The present study aimed to investigate the potential of rabbit BMSCs in osteogenesis after the transfection of human BMP-2 and EGFP recombinant adenovirus. Rabbit BMSCs were isolated and the surface stem cell makers, including CD29, CD44 and CD45 were detected by flow cytometry. The expression of BMP-2 mRNA and protein in BMSCs were detected by reverse transcription-quantitative polymerase chain reaction and western blot...
Source: Experimental and Therapeutic Medicine - November 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Protective effect of dexpanthenol against cisplatin-induced hepatotoxicity.
Authors: Bilgic Y, Akbulut S, Aksungur Z, Erdemli ME, Ozhan O, Parlakpinar H, Vardi N, Turkoz Y Abstract The aim of the present study was to investigate the protective effect of dexpanthenol (Dexp) against cisplatin (Cis)-induced hepatotoxicity. Thirty-two Sprague Dawley rats were divided into four groups: Control group (n=8), Dexp group (n=8, 500 mg/kg/ip/daily single dose/3 days Dexp), Cis group (n=8, 7 mg/kg/ip/single dose Cis) and Cis+Dexp group (n=8, 500 mg/kg/ip/daily single dose/3 days Dexp +7 mg/kg/ip/single dose Cis). MDA, CAT, GSH, GSH-Px, TOS, TAS, OSI, Total Nitrit, IL-1β, IL-6 and TNF-α leve...
Source: Experimental and Therapeutic Medicine - November 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Intraoperative and postoperative infusion of dexmedetomidine combined with intravenous butorphanol patient-controlled analgesia following total hysterectomy under laparoscopy.
Authors: Du J, Li JW, Jin J, Shi CX, Ma JH Abstract The present prospective, randomized, double-blinded, controlled study aimed to investigate the efficacy and safety of dexmedetomidine (DEX) combined with butorphanol for patient-controlled intravenous analgesia (PCIA) following total laparoscopic hysterectomy. A total of 88 patients undergoing total laparoscopic hysterectomy and receiving postoperative PCIA were divided into two groups following surgery. Patients received DEX 0.5 µg/kg intravenously in the DEX group or 0.9% normal saline in the control (CON) group following anesthesia induction. Postoperativ...
Source: Experimental and Therapeutic Medicine - November 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Expression of tissue factor in human cervical carcinoma tissue.
In conclusion, TF is highly expressed in cervical cancer tissues and high expression of TF may enhance the invasion and metastasis of cervical cancer cells. PMID: 30402151 [PubMed] (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - November 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Calcimimetic R568 reduced the blood pressure and improved aortic remodeling in spontaneously hypertensive rats by inhibiting local renin-angiotensin system activity.
In this study, we treated SHR and Wistar-Kyoto rats with R568 for 8 weeks. The tail-cuff method was used to assess rat BP weekly. Morphological changes in the thoracic aorta were evaluated with hematoxylin-eosin and Masson staining. Western blotting and immunohistochemistry were used to detect the expression of RAS-related proteins and proliferative remodeling proteins in the thoracic aorta. An enzyme-linked immunosorbent assay was used to detect the content of cAMP, the RAS, and the CaSR in plasma and the thoracic aorta. Finally, we found that treatment with R568 for 8 weeks reduced the BP and inhibited arterial vascular ...
Source: Experimental and Therapeutic Medicine - November 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Ginsenoside Rg1 attenuates liver injury induced by D-galactose in mice.
Authors: Xiao MH, Xia JY, Wang ZL, Hu WX, Fan YL, Jia DY, Li J, Jing PW, Wang L, Wang YP Abstract The present study investigated the effect and underlying mechanisms of ginsenoside Rg1 (Rg1) in attenuating subacute liver injury induced by D-galactose (D-gal) in mice. Specific Pathogen Free (SPF) male C57BL/6J mice were randomly divided into 3 groups: i) D-gal-administration group (D-gal group), where the mice were intraperitoneally administrated with D-gal (120 mg/kg/day for 42 days); ii) D-gal + Rg1 group where the mice were treated with 120 mg/kg/day D-gal for 42 days and with Rg1 at a dose of 20 mg/kg/day for 35...
Source: Experimental and Therapeutic Medicine - November 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Therapeutic effect of irbesartan combined with atorvastatin calcium in the treatment of rats with coronary heart disease.
This study aimed to investigate the therapeutic effect of irbesartan combined with atorvastatin calcium in the treatment of rats with coronary heart disease. One hundred sixty Wistar rats were selected to establish coronary heart disease model. Rats with coronary heart disease were randomly divided into 4 groups: Model, irbesartan, atorvastatin calcium and combination groups (irbesartan combined with atorvastatin calcium group). Rats in irbesartan group were treated with 50 mg/(kg.day) irbesartan; rats in atorvastatin calcium group were given atorvastatin calcium at a dose of 10 mg/(kg.day); rats in combination group were ...
Source: Experimental and Therapeutic Medicine - November 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Comparison of the predictive value of four-dimensional speckle tracking imaging risk classification and the TIMI system after STEMI reperfusion therapy.
Authors: Wang Y, Xu R, Yin X, Wang J, Feng L, Zhou J Abstract The predictive value of four-dimensional speckle tracking imaging (4D-STI) risk classification and TIMI risk scores for the prognosis of patients with ST-segment elevation myocardial infarction (STEMI) after reperfusion therapy were investigated. One hundred and twenty patients with STEMI after reperfusion therapy were involved. At 12 h after reperfusion therapy, 2nd and 3rd day, the three-dimensional longitudinal strain (LS), circumferential strain (CS) and radial strain (RS), area strain (AS), as well as other 4D-STI detection indicators, were collecte...
Source: Experimental and Therapeutic Medicine - November 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Outcomes of endovascular interventional therapy for primary Budd-Chiari syndrome caused by hepatic venous obstruction.
Authors: Cheng DL, Zhu N, Xu H, Li CL, Lv WF, Fang WW, Li CT Abstract To date, interventional therapy for patients with Budd-Chiari syndrome (BCS) due to hepatic vein obstruction (HVO) has not been standardized in China. In Western countries, BCS primarily occurs due to thrombosis and the majority of patients receive thrombolysis. In China, BCS is mostly caused by the membranous occlusion of the HV or IVC. The present retrospective study evaluated the efficacy of recanalization techniques in patients with primary BCS due to HVO. The data of 69 patients with BCS due to HVO, who underwent endovascular therapy at 2 ce...
Source: Experimental and Therapeutic Medicine - November 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Correlation of vascular endothelial function and coagulation factors with renal function and inflammatory factors in patients with diabetic nephropathy.
Authors: Sun J, Liu C Abstract Correlation of vascular endothelial function and coagulation factors with renal function and inflammatory factors in patients with diabetic nephropathy was analyzed. A total of 86 patients diagnosed with diabetes mellitus (DM) and admitted to the 89th Hospital of the People's Liberation Army (Weifang, China) from March 2014 to May 2017 were selected. Among them, 38 patients complicated with nephropathy were divided into the observation group and 48 patients without nephropathy into the control group. The general data of patients were collected, and the relevant biochemical indexes, va...
Source: Experimental and Therapeutic Medicine - November 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Human nasopharyngeal carcinoma can be radiosensitized by trichosanthin via inhibition of the PI3K pathway.
In conclusion, TCS radiosensitized NPC in vitro and in vivo via downregulation of PI3K pathways and the upregulation of cleaved caspase-3. PMID: 30402158 [PubMed] (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - November 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

IMB-6G induces endoplasmic reticulum stress-mediated apoptosis in human nasopharyngeal carcinoma cells.
In conclusion, the present findings indicated that IMB-6G induced ER stress-mediated apoptosis through activating IRE1α and PERK signaling pathways. The present study suggests that IMB-6G may be a promising agent for NPC treatment. PMID: 30402159 [PubMed] (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - November 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Distinguishing the dominant species of pathogen in ethmoidal sinusitis by sequencing DNA dataset analysis.
Authors: Zhang J, He S, Li Y, Lv M, Wei H, Qu B, Zheng Y, Hu C Abstract Identifying the predominant microbial species in patients with ethmoidal sinusitis is conducive to its successful treatment. The aim of the present study was to determine the microbial composition and the predominant fungal and bacterial species in patients with ethmoidal sinusitis. A sample was obtained from 3 patients with ethmoidal sinusitis and from the ethmoid sinus of 2 healthy volunteers. Those samples were sequenced using an Illumina/Solexa sequencing platform for mapping to human, fungal, and bacterial genomes. Fungal and bacterial exp...
Source: Experimental and Therapeutic Medicine - November 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

miR-365b-3p inhibits the cell proliferation and migration of human coronary artery smooth muscle cells by directly targeting ADAMTS1 in coronary atherosclerosis.
Authors: Qu Y, Zhang N Abstract Abnormal proliferation and migration of vascular smooth muscle cells serves a crucial role in the development of atherosclerosis. Previous studies have suggested that some microRNAs (miRs) are involved in this process; however, the associated underlying molecular mechanism is unclear. In present study, human coronary artery smooth muscle cells (HCASMCs) were used to explore the function of miR-365b-3p in the coronary atherosclerosis. It was indicated that platelet-derived growth factor-BB (PDGF-BB) treatment inhibited miR-365b-3p expression and upregulated the expression of a disinte...
Source: Experimental and Therapeutic Medicine - November 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Down-regulated G protein-coupled receptor kinase 6 leads to apoptosis in multiple myeloma MM1R cells.
Authors: Zhang Z, Li Z, Chen W Abstract G protein-coupled receptor kinase 6 (GRK6) is highly expressed in multiple myeloma (MM) cell lines, but absent or only weakly expressed in most primary human somatic cells. In the present study, GRK6 expression was assessed in MM patients and healthy individuals by quantitative polymerase chain reaction. Flow cytometry were performed to measure the apoptosis of lentivial-transfected MM1R cells. Western blot analysis was performed to assess the apoptosis and signal transducer and activator of transcription 3 pathway-related factors. The results demonstrated that GRK6 was diffe...
Source: Experimental and Therapeutic Medicine - November 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Electrocardiography-gated dual-source computed tomography in the detection of atrial septal aneurysm.
Authors: Xie L, Liu Z, Zhang X, Xu K, Xu Q, Lu L, Hu C, Han S, Li J Abstract The aim of the current study was to investigate the atrial septal aneurysm (ASA) incidence rate and describe its morphologic features by dual-source computed tomography (DSCT). A total of 8,626 patients (4,284 men and 4,342 women) who underwent DSCT examinations were enrolled. The presence of ASA was defined as the protrusion of the complete or partial interatrial septum with a maximum vertical distance (MVD) from the atrial septum plane of>10 mm during a cardiac cycle and a diameter of the aneurysm base of>15 mm. The MVD and directi...
Source: Experimental and Therapeutic Medicine - November 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Optimal sampling time-point for cyclosporin A concentration monitoring in heart transplant recipients.
In conclusion, C2 is an ideal time-point for monitoring plasma CsA levels with a utility for individualising the next scheduled dose for each patient to ensure that target levels are maintained and achieve a high efficacy and safety of CsA therapy in heart transplant recipients (clinical trial no. 12002610). PMID: 30402164 [PubMed] (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - November 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Anti-inflammatory effect of Resolvin D1 on LPS-treated MG-63 cells.
In conclusion, The present study demonstrated that RvD1 inhibited inflammation though inhibiting MAPKp38/NF-κB pathway and NLRP3 inflammasome expression in MG-63 cells, indicating that it may be an effective drug for the treatment of ankle fracture. PMID: 30402165 [PubMed] (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - November 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Annexin A2 and FTH1 are potential biomarkers for lupus nephritis.
Authors: Zhou Y, Xiao L, Tang S Abstract Lupus nephritis (LN) occurs in ~50% of patients with systemic lupus erythematosus and is a major cause of morbidity and mortality of the affected individuals. Therefore, identification of novel and predictive biomarkers for the early diagnosis and progression of LN is required. The present study included 10 patients with LN whose diagnoses were confirmed by renal biopsy and 5 healthy participants as control subjects. Sera were collected both from patients with LN and healthy controls. Subsequently, mesangial cells were treated with these sera for 24 h. Differential proteins ...
Source: Experimental and Therapeutic Medicine - October 24, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Atorvastatin upregulates apolipoprotein M expression via attenuating LXR α expression in hyperlipidemic apoE-deficient mice.
Atorvastatin upregulates apolipoprotein M expression via attenuating LXRα expression in hyperlipidemic apoE-deficient mice. Exp Ther Med. 2018 Nov;16(5):3785-3792 Authors: Liu J, Huang H, Shi S, Wang X, Yu Y, Hu Y, Sun J, Ren C, Yang J, Shen Z Abstract Apolipoprotein M (apoM) is a recently identified human apolipoprotein that is associated with the formation of high-density lipoprotein (HDL). Studies have demonstrated that statins may affect the expression of apoM; however, the regulatory effects of statins on apoM are controversial. Furthermore, the underlying mechanisms by which statins regulat...
Source: Experimental and Therapeutic Medicine - October 24, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

The relationship between inflammatory factor expression and blood pressure and urinary protein in the placenta of gestational hypertension rats.
Authors: Jia XQ, Xu S, Tian MR, Ma YY Abstract The association between inflammatory factor expression and blood pressure with urinary protein in the placenta of pregnant women with pregnancy-induced hypertension (PIH) was investigated to provide a new vision for the clinical prevention and treatment of PIH. Rats were used as animal models and were randomly divided into three groups (control, hypertension and treatment groups) on day 15 of pregnancy with 20 rats in each group. The 10% hypoxia-induced PIH group was induced with administration of an anti-hypertensive drug, and the treatment group was giventreprostinil...
Source: Experimental and Therapeutic Medicine - October 24, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Analysis of curative effect of adjuvant therapy with bronchoalveolar lavage on COPD patients complicated with pneumonia.
Authors: Zhao H, Gu H, Liu T, Ge J, Shi G Abstract Clinical effect of adjuvant therapy with bronchoalveolar lavage on chronic obstructive pulmonary disease (COPD) patients complicated with pneumonia and its influence on the expression levels of inflammatory factors were studied. One hundred and twenty mild-moderate COPD patients complicated with pneumonia treated in the Department of Respiratory Medicine, The Sixth People's Hospital of Nantong from February 2016 to February 2017 were selected and randomly divided into three groups: One-time lavage group (n=40), two-time lavage group (n=40) and control group (n=40)....
Source: Experimental and Therapeutic Medicine - October 24, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Baseline serum miR-125b levels predict virologic response to nucleos(t)ide analogue treatment in patients with HBeAg-positive chronic hepatitis B.
Authors: Zhou P, Dong M, Wang J, Li F, Zhang J, Gu J Abstract The aim of the present study was to investigate the predictive value of baseline serum microRNA (miRNA)-125b for nucleos(t)ide analogues (NAs) in patients with chronic hepatitis B (CHB). A total of 66 patients with Be antigen (HBeAg)-positive CHB received NAs therapy for 144 weeks. Serum miRNA-125b levels were measured at the baseline, while hepatitis B virus (HBV) DNA, hepatitis B surface antigen (HBsAg) and alanine aminotransferase (ALT) levels were measured throughout treatment. Stepwise logistic regression analysis was performed to identify predictor...
Source: Experimental and Therapeutic Medicine - October 24, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Regulatory T-cell density and cytotoxic T lymphocyte density are associated with complete response to neoadjuvant paclitaxel and carboplatin chemoradiotherapy in gastric cancer.
In conclusion, these outcomes indicated that regulatory T-cell density and cytotoxic T lymphocyte density in the tumor microenvironment were associated with the response to neoadjuvant CRT and may represent a therapeutic target for gastric cancer. PMID: 30344657 [PubMed] (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - October 24, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Correlation of GSTM1 gene deletion in joint synovial fluid with the recovery of patients undergoing artificial hip replacement.
Authors: Lin X, Xu T, Wu B, Hu B, Qin M Abstract The present study was designed to investigate the correlation between glutathione S-transferase M1 (GSTM1) gene polymorphism and the recovery of patients undergoing artificial hip replacement. A total of 241 patients including 149 males (61.8%) and 92 females (38.9%) who received artificial hip replacement in People's Hospital of Rizhao between December 2010 and October 2016 were enrolled to serve as the observation group. Patients were divided into two subgroups according to the loss of GSTM1. A total of 80 healthy subjects who udenrwent a physical examination in ou...
Source: Experimental and Therapeutic Medicine - October 24, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

MGCD0103 induces apoptosis and simultaneously increases the expression of NF- κB and PD-L1 in classical Hodgkin's lymphoma.
MGCD0103 induces apoptosis and simultaneously increases the expression of NF-κB and PD-L1 in classical Hodgkin's lymphoma. Exp Ther Med. 2018 Nov;16(5):3827-3834 Authors: Huang R, Zhang X, Min Z, Shadia AS, Yang S, Liu X Abstract At present there is no consensus on the treatment of classical Hodgkin's lymphoma (CHL) following relapse. The aim of the present study was to access the class I-selective histone deacetylase (HDAC) inhibitor (HDACI) MGCD0103 on the expression levels of Bcl-2, nuclear factor (NF)-κB and programmed death-ligand 1 (PD-L1) in CHL, to explore the possible therapeutic v...
Source: Experimental and Therapeutic Medicine - October 24, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Influence of intrathecal injection with dexmedetomidine on the behavioral ability and analgesic effects on rats with neuropathic pain and expression of protein kinase C in the spinal dorsal horn.
This study aimed to investigate the influence of intrathecal injection with dexmedetomidine on the behavioral ability and analgesic effects of rat models with chronic neuropathic pain. It also discusses the role that the expression of protein kinase C (PKC) in the spinal dorsal horn plays in the analgesic mechanism of dexmedetomidine. A total of 35 Sprague Dawley rats were selected. Five rats were randomly chosen as the blank control group and the rest were prepared as models with chronic constriction injury, which were divided into the model and the observation group (n=15, each group). The observation group was injected ...
Source: Experimental and Therapeutic Medicine - October 24, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Dynamics study on the role of curcumin on TGF- β1 expression and pathological changes in acute paraquat poisoned rats.
Dynamics study on the role of curcumin on TGF-β1 expression and pathological changes in acute paraquat poisoned rats. Exp Ther Med. 2018 Nov;16(5):3841-3846 Authors: Chen H, Fu X Abstract Role of curcumin on TGF-β1 expression and pathological changes in acute paraquat (PQ) poisoned rats were investigated. Forty-eight SD rats were divided into three groups: control group, PQ group, and PQ + curcumin group, with 16 rats in each group. PQ group rats received the lavagation of PQ every day, PQ + curcumin group was given the lavagation of curcumin treatment on the basis of the PQ group. Control gr...
Source: Experimental and Therapeutic Medicine - October 24, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

An improved surgical procedure to establish a gastroesophageal reflux model with a high incidence of Barrett's esophagus in rats.
Authors: Wen H, Liu T, Liu H, Teng JH, Li SB Abstract Barrett's esophagus (BE) is a complication of gastroesophageal reflux disease and is a precursor lesion of esophageal adenocarcinoma. In existing BE models, the incidence of BE is typically low and induction is usually time consuming. In the present study, a gastroesophageal reflux model with a high incidence of BE in rats. Rats were divided into a model group and a sham operation group, and anesthetized with an inhalation anesthesia machine. Stomach-jejunal anastomosis (SJA) and esophagus-jejunal anastomosis (EJA) were simultaneously performed in the model grou...
Source: Experimental and Therapeutic Medicine - October 24, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Effects of dexmedetomidine on perioperative stress response, inflammation and immune function in patients with different degrees of liver cirrhosis.
Authors: Wang L, Zhang A, Liu W, Liu H, Su F, Qi L Abstract Effects of dexmedetomidine (DEX) on perioperative stress response, inflammation and immune function in patients with different degrees of liver cirrhosis were investigated. A total of 94 patients with liver cirrhosis who were admitted to the Affiliated Hospital of Shandong University of Traditional Chinese Medicine from December 2016 to November 2017 were included, and randomly divided into control and observation group (n=47). Patients in control group were given remifentanil for anesthesia, while patients in observation group were treated with remifentan...
Source: Experimental and Therapeutic Medicine - October 24, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

NF- κB inhibitor on Toll-like receptor 4 signal-induced expression of angiotensinogen and AT1a receptor in neonatal rat left ventricular myocytes.
NF-κB inhibitor on Toll-like receptor 4 signal-induced expression of angiotensinogen and AT1a receptor in neonatal rat left ventricular myocytes. Exp Ther Med. 2018 Nov;16(5):3875-3882 Authors: Jiang H, Wang HY, Wang JW, Lou DY, Niu N, Li GH, Qu P Abstract Effects of toll-like receptor 4 (TLR4)/nuclear factor-κB (NF-κB) pathway on expression of angiotensinogen and AT1a receptor were investigated, to explore the role of TLR4/NF-κB signaling pathway in cardiovascular disease. Neonatal rat left ventricular myocytes (NRVMs) were cultured and cardiomyocytes were identified by immunoc...
Source: Experimental and Therapeutic Medicine - October 24, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Clinical outcomes of arthroscopic synovectomy for adolescent or young adult patients with advanced haemophilic arthropathy.
Authors: Zhang T, Huang S, Xu S, Li H, He X, Zhang F Abstract The aim of the present study was to assess the clinical outcomes of arthroscopic synovectomy in adolescent or young adult patients with advanced haemophilic arthropathy. From January 2009-January 2012, clinical data from 11 adolescent or young adult patients with advanced haemophilic arthropathy who were treated with arthroscopic synovectomy were retrospectively collected. The mean follow-up period was 71.91±5.28 months. The evaluated indicators included frequency of joint bleeding, range of motion (ROM), X-ray staging, hospital for special surger...
Source: Experimental and Therapeutic Medicine - October 24, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Transversus abdominis plane block reduces remifentanil and propofol consumption, evaluated by closed-loop titration guided by bispectral index.
Authors: Ma J, Wang XY, Sun QX, Zhou J, Li T, Jiang MR, Liu GG, Liu H Abstract The present prospective, randomized, double-blind study aimed to determine the impact of transversus abdominis plane (TAP) block on propofol and remifentanil consumption, when administered by closed-loop titration guided by processed electroencephalography, i.e., bispectral index (BIS) values. Following institutional review board approval, 60 patients were scheduled for laparoscopic colectomy under general anesthesia. Patients were randomly assigned to receive bilateral TAP block with 20 ml 0.375% ropivacaine (TAP group) or 20 ml 0.9% sa...
Source: Experimental and Therapeutic Medicine - October 24, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Dynamic protein expression of NF- κB following rat intracerebral hemorrhage and its association with apoptosis.
Dynamic protein expression of NF-κB following rat intracerebral hemorrhage and its association with apoptosis. Exp Ther Med. 2018 Nov;16(5):3903-3908 Authors: Liu ZC, Meng LQ, Song JH, Gao J Abstract The aim of the present study was to evaluate the dynamic protein expression of nuclear factor (NF)-κB and apoptosis in the cerebral tissue surrounding hematoma following intracerebral hemorrhage (ICH) in rats. A total of 80 healthy male Wistar rats were divided into a sham-surgery group and an ICH group. The ICH model was established by injecting autogenous non-heparin anticoagulant arterial bl...
Source: Experimental and Therapeutic Medicine - October 24, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Therapeutic effect of photodynamic therapy combined with imiquimod in the treatment of anal condyloma acuminatum and its effect on immune function.
Authors: Meng X, Li Y, Luan H, Shi X Abstract Therapeutic effect of photodynamic therapy combined with imiquimod in the treatment of anal condyloma acuminatum (CA) was investigated to explore its effect on immune function. A total of 104 patients with anal CA were randomly divided into two groups: Patients in the study group were treated with photodynamic therapy combined with imiquimod, and ii) patients in the control group were treated with recombinant human interferon α-2b cream. Clinical efficacy and immune function related indicators were compared between the two groups. After treatment for 6 weeks, the ...
Source: Experimental and Therapeutic Medicine - October 24, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Upregulation of miRNA-140-5p inhibits inflammatory cytokines in acute lung injury through the MyD88/NF- κB signaling pathway by targeting TLR4.
Upregulation of miRNA-140-5p inhibits inflammatory cytokines in acute lung injury through the MyD88/NF-κB signaling pathway by targeting TLR4. Exp Ther Med. 2018 Nov;16(5):3913-3920 Authors: Yang Y, Liu D, Xi Y, Li J, Liu B, Li J Abstract The present study was designed to determine the effect of miR-140-5p on acute lung injury (ALI) and the associated inflammation induced. As a result, miR-140-5p expression in mice with ALI was suppressed when compared with the normal group. Downregulation of miR-140-5p increased the levels of inflammatory factors induced by ALI [including tumor necrosis factor-&...
Source: Experimental and Therapeutic Medicine - October 24, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

UNBS5162 induces growth inhibition and apoptosis via inhibiting PI3K/AKT/mTOR pathway in triple negative breast cancer MDA-MB-231 cells.
Authors: Yue X, Li M, Chen D, Xu Z, Sun S Abstract UNBS5162, a naphthalimide derivative, has a strong anticancer effect and low clinical hematological toxicity. UNBS5162 has been shown to have an anticancer effect in numerous cancer types, except triple negative breast cancer (TNBC). To explore this issue, TNBC MDA-MB-231 cells were selected, and the effect of UNBS5162 on their growth, proliferation and motility regulation was investigated in vitro. The results showed that UNBS5162 significantly inhibited the proliferation, migration and invasion of TNBC cells. In addition, the level of pro-apoptosis protein Bcl-2-...
Source: Experimental and Therapeutic Medicine - October 24, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Therapeutic effect of Rho kinase inhibitor FSD-C10 in a mouse model of Alzheimer's disease.
Authors: Gu QF, Yu JZ, Wu H, Li YH, Liu CY, Feng L, Zhang GX, Xiao BG, Ma CG Abstract Fasudil, a Rho kinase (ROCK) inhibitor, effectively inhibits disease severity in a mouse model of Alzheimer's disease (AD). However, given its significant limitations, including a relatively narrow safety window and poor oral bioavailability, Fasudil is not suitable for long-term use. Thus, screening for ROCK inhibitor(s) that are more efficient, safer, can be used orally and suitable for long-term use in the treatment of neurodegenerative disorders is required. The main purpose of the present study is to explore whether FSD-C10, ...
Source: Experimental and Therapeutic Medicine - October 24, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Diosgenin protects against alveolar bone loss in ovariectomized rats via regulating long non-coding RNAs.
Authors: Zhang Z, Chen Y, Xiang L, Wang Z, Xiao GG, Ju D Abstract The present study assessed the changes in long non-coding (lnc)RNA and mRNA expression profiles when diosgenin (DIO) exerted a potential osteoprotective effect on the alveolar bone of ovariectomized (OVX) rats. Female Wistar rats underwent a sham operation (SHAM group) or ovariectomy. OVX rats were treated using vehicle (OVX group), DIO (DIO group) or estradiol valerate (EV group) for 12 weeks. After treatment, the biomarkers of bone turnover in plasma and the microstructure of alveolar bone were assessed. lncRNA microarrays were applied to assess ln...
Source: Experimental and Therapeutic Medicine - October 24, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

lncRNA Malat1 modulates the maturation process, cytokine secretion and apoptosis in airway epithelial cell-conditioned dendritic cells.
Authors: Li Z, Zhang Q, Wu Y, Hu F, Gu L, Chen T, Wang W Abstract Airway epithelial cells (AECs) are the first point of contact with airborne antigens and are able to instruct resident immune cells to appropriate immune responses. Previous studies have shown that the abnormal expression of metastasis-associated lung adenocarcinoma transcript 1 (Malat1) was associated with tumorigenesis, progression, metastasis, and apoptosis in many cancer types. However, little is known about its functional involvement in the cross-talk of AECs with dendritic cells (DCs). The aim of the present study was to identify Malat1 as a no...
Source: Experimental and Therapeutic Medicine - October 24, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Inhibition of peptidyl-prolyl cis-trans isomerase B mediates cyclosporin A-induced apoptosis of islet β cells.
Inhibition of peptidyl-prolyl cis-trans isomerase B mediates cyclosporin A-induced apoptosis of islet β cells. Exp Ther Med. 2018 Nov;16(5):3959-3964 Authors: Wei X, Zhu D, Feng C, Chen G, Mao X, Wang Q, Wang J, Liu C Abstract Cyclosporin A (CsA) is widely used as an immunosuppressor in the context of organ transplantation or autoimmune disorders. Recent studies have revealed the detrimental effects of CsA on insulin resistance and pancreatic β cell failure; however, the molecular mechanisms are unknown. The present study sought to confirm the associations between CsA and β cell failure,...
Source: Experimental and Therapeutic Medicine - October 24, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Highly selective peripheral nerve radio frequency ablation for the treatment of severe knee osteoarthritis.
The objective of the present study was to investigate the effect of highly selective peripheral nerve radiofrequency (RF) ablation for the pain caused by severe knee osteoarthritis (OA). A total of 96 patients with knee OA were randomly divided into two groups. The 49 patients in group A were treated with highly selective peripheral nerve RF ablation group and the 47 patients in group B were treated with sodium hyaluronate injection. Visual analogue scale (VAS), Lysholm knee score (LKS), and pain relief before treatment and at 3 days and 3, 6, 9, and 12 months after treatment were compared. At each time point after treatme...
Source: Experimental and Therapeutic Medicine - October 24, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Construction of lncRNA-miRNA-mRNA networks reveals functional lncRNAs in abdominal aortic aneurysm.
Authors: Tian L, Hu X, He Y, Wu Z, Li D, Zhang H Abstract Abdominal aortic aneurysm (AAA) is one of the most significant causes of morbidity and mortality in populations aged>65 years worldwide. However, the underlying mechanisms of AAA based on the competitive endogenous RNA (ceRNA) hypothesis have remained elusive. In the present study, differently expressed long non-coding RNA (lncRNA)-microRNA (miRNA)-mRNA networks in AAA were constructed by analyzing public datasets, including GSE7084, GSE24194 from rats and that of a previous study. A total of 1,219 mRNAs, 2,093 lncRNAs and 57 miRNAs were identified to dif...
Source: Experimental and Therapeutic Medicine - October 24, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research