PHLDA3 inhibition attenuates endoplasmic reticulum stress-induced apoptosis in myocardial hypoxia/reoxygenation injury by activating the PI3K/AKT signaling pathway
In conclusion, in the process of H/R injury, PHLDA3 inhibition reduced ERS-induced apoptosis and H/R injury by activating the PI3K/AKT pathway. PHLDA3 may be a therapeutic target for the treatment of MIRI.PMID:33936270 | PMC:PMC8082641 | DOI:10.3892/etm.2021.10045 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Kai Liu Ying Chen Fen Ai Yun-Qian Li Kun Zhang Wei-Tong Zhang Source Type: research

Novel ARID1B variant inherited from somatogonadal mosaic mother in siblings with Coffin-Siris syndrome 1
Exp Ther Med. 2021 Jun;21(6):614. doi: 10.3892/etm.2021.10046. Epub 2021 Apr 14.ABSTRACTCoffin-Siris syndrome1 (CSS1; Online Mendelian Inheritance in Man no. 135900) is a multiple malformation syndrome characterized by intellectual and/or developmental delay, and hypoplastic or absent fifth fingernails and/or toenails. AT-rich interaction domain-containing protein 1B (ARID1B) is the most frequently mutated gene in CSS1 and the majority of reported cases have been sporadic. Using whole-exome sequencing, the present study identified two siblings with CSS1 with a novel heterozygous co-segregating pathogenic variant in the ARI...
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Zhong Min Cheng Qian Dai Ying Source Type: research

Exogenous transforming growth factor- β1 prevents the inflow of fluoride to ameleoblasts through regulation of voltage-gated chloride channels 5 and 7
Exp Ther Med. 2021 Jun;21(6):615. doi: 10.3892/etm.2021.10047. Epub 2021 Apr 14.ABSTRACTDental fluorosis is a global issue. Although there are multiple causes of dental fluorosis, the precise mechanism remains controversial. Previous studies have demonstrated that extracellular fluoride may promote an accumulation of fluoride ions in ameloblasts, which may induce oxidative and endoplasmic reticulum stresses, leading to dental fluorosis. However, the exact process by which fluoride ions enter cells has not been determined. In the present study, intracellular fluoride concentration was determined using a newly developed spec...
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Mei Ji Xuejing Duan Xiaohui Han Jing Sun Dongsheng Zhang Source Type: research

ΜicroRNA-122 protects against ischemic stroke by targeting Maf1
In conclusion, the results suggested that miR-122 improved the outcome of acute ischemic stroke by reducing the expression of Maf1.PMID:33936273 | PMC:PMC8082594 | DOI:10.3892/etm.2021.10048 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Mengmeng Wang Xiaoman Liu Yu Wu Yi Wang Jiahui Cui Jing Sun Ying Bai Ming-Fei Lang Source Type: research

Changes in bone mineral density, 25-hydroxyvitamin D < sup > 3 < /sup > and inflammatory factors in patients with hyperthyroidism
Exp Ther Med. 2021 Jun;21(6):617. doi: 10.3892/etm.2021.10049. Epub 2021 Apr 14.ABSTRACTThe present study aimed to evaluate changes in bone mineral density, 25-hydroxyvitamin D3 [25-(OH)D3] and inflammatory factors in patients with hyperthyroidism, in order to determine the correlations with the pathogenesis of hyperthyroidism. A total of 55 patients with hyperthyroidism (observation group) and 53 healthy patients (control group) enrolled at Weifang People's Hospital from March 2017 to February 2018 were randomly enrolled. The thyroid function, bone mineral density, 25-(OH)D3 and inflammatory factors were measured and comp...
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Yali Zhou Xixia Wang Maoyuan Xin Haiting Zhuang Source Type: research

Total flavonoids of < em > Rhizoma Drynariae < /em > combined with calcium attenuate osteoporosis by reducing reactive oxygen species generation
Exp Ther Med. 2021 Jun;21(6):618. doi: 10.3892/etm.2021.10050. Epub 2021 Apr 14.ABSTRACTIn the present study, the effects of total flavonoids of Rhizoma Drynariae (TFRD) and calcium carbonate (CaCO3) on osteoporosis (OP) were assessed in a rat model of OP. For this purpose, 36 Sprague-Dawley rats, aged 3 months, were randomly divided into a group undergoing sham surgery (sham-operated group), model group (OP group), CaCO3 group (OP + CaCO3 group), TFRD group (OP + TFRD group), TFRD combined with CaCO3 group (OP + TFRD + CaCO3 group) and TFRD and CaCO3 combined with N-acetyl cysteine group (OP + TFRD + CaCO3 + NAC group). T...
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Panyun Mu Yimei Hu Xu Ma Jingru Shi Zhendong Zhong Lingyuan Huang Source Type: research

Polycomb group protein Bmi1 is required for the neuronal differentiation of mouse induced pluripotent stem cells
Exp Ther Med. 2021 Jun;21(6):619. doi: 10.3892/etm.2021.10051. Epub 2021 Apr 14.ABSTRACTInduced pluripotent stem cells (iPSCs) reprogrammed by somatic cells may be used as a potentially novel treatment regimen in stem cell regenerative medicine, particularly in the central nervous system (CNS). In the present study, iPSCs were generated using mouse embryonic fibroblasts by ectopic overexpression of Sox-2, Oct-3/4, Klf-4 and c-Myc, and cultured under the same conditions as that used for embryonic stem cells. The neuronal differentiation capacity of mouse iPSCs was examined, and the involvement of the formation of embryoid ...
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Wei Shan Liping Zhou Lizhen Liu Deju Lin Qin Yu Source Type: research

Transforming growth factor- β1-induced podocyte injury is associated with increased microRNA-155 expression, enhanced inflammatory responses and MAPK pathway activation
Exp Ther Med. 2021 Jun;21(6):620. doi: 10.3892/etm.2021.10052. Epub 2021 Apr 14.ABSTRACTMicroRNA-155 (miR-155) is associated with various diseases. However, the potential role of miR-155 in early glomerular disease (EGD) remains elusive. In the present study, the clinical significance of urinary miR-155 expression was explored in patients with EGD using receiver operating characteristic curve analysis. Conditionally immortalized mouse podocytes were cultured in vitro and treated with transforming growth factor-β1 (TGF-β1) at different concentrations and durations. The gene expression levels of mRNAs and miR-155 w...
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Xintong Zheng Qiuhong Zhong Xu Lin Xianjun Gu Xiaoyan Ling Zhao Liang Qing Qin Xiuri Du Source Type: research

Joint line plate fixation for tibial plateau fractures caused by hyperextension varus
In conclusion, internal fixation with a joint line plate is an appropriate treatment for tibial plateau fractures involving the anteromedial margin with good clinical efficacy.PMID:33936278 | PMC:PMC8082576 | DOI:10.3892/etm.2021.10053 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Yu-Cheng Huang Jing Jiao Wen-Jun Cheng Fei Xiao Wei Zuo Jun-Wen Wang Source Type: research

miR-491-5p inhibits the proliferation and migration of A549 cells by FOXP4
Exp Ther Med. 2021 Jun;21(6):622. doi: 10.3892/etm.2021.10054. Epub 2021 Apr 14.ABSTRACTAberrant expression of microRNAs (miRNAs/miRs) plays a key role in the development of non-small cell lung cancer (NSCLC). In the present study, lower miRNA (miR)-491-5p levels and a higher forkhead box P4 (FOXP4) mRNA level were observed in NSCLC tissues and cell lines, compared to adjacent tissues and the normal human lung epithelial cell line BEAS-2B, respectively. A549 cell proliferation and migration were inhibited upon transfection of miR-491-5p mimics compared to miR-negative control (NC) mimics. In addition, compared to miR-NC mi...
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Fuyong Wu Aiping Ji Zhenkun Zhang Jinfang Li Penglong Li Source Type: research

Effect of media distraction (audio-visual and music) for pain and anxiety control in patients undergoing shock-wave lithotripsy: A systematic review and meta-analysis
Exp Ther Med. 2021 Jun;21(6):623. doi: 10.3892/etm.2021.10055. Epub 2021 Apr 14.ABSTRACTAudio-visual (AV) or music distraction may be used to reduce pain during several healthcare procedures. The present manuscript is a systematic review and meta-analysis to assess the effectiveness of media distraction in reducing pain and anxiety in extracorporeal shock wave lithotripsy (ESWL) patients. The PubMed, Embase, Scopus, BioMed Central, Ovoid and CENTRAL (Cochrane Central Register of Controlled Trials) databases were screened for studies assessing the role of media distraction (music/AV media) in reducing pain and anxiety of ES...
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Weihua Hu Ke Yang Li Zhang Xu Lu Source Type: research

Chemical hemorrhagic cystitis: Diagnostic and therapeutic pitfalls (Review)
In conclusion, CC is a disease with an increasing incidence, challenging to diagnose, which is frequently mistreated, and has multiple treatment modalities that still require standardization in administration and sequencing.PMID:33936281 | PMC:PMC8082586 | DOI:10.3892/etm.2021.10056 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Razvan-Cosmin Petca Razvan-Ionut Popescu Cristian Toma Mihai Cristian Dumitrascu Aida Petca Florica Sandru Calin Bogdan Chibelean Source Type: research

NICE-3-knockdown induces cell cycle arrest and autophagy in lung adenocarcinoma cells via the AKT/mTORC1 signaling pathway
Exp Ther Med. 2021 Jun;21(6):625. doi: 10.3892/etm.2021.10057. Epub 2021 Apr 15.ABSTRACTThe NICE-3 protein serves an oncogenic role in hepatocellular carcinoma, but its role in lung adenocarcinoma (LUAD) remains unknown. The aim of the present study was to investigate the potential role and underlying mechanisms of NICE-3 in LUAD. In the present study, NICE-3 expression in LUAD tissues and its association with patient prognosis were analyzed using datasets from The Cancer Genome Atlas and Gene Express Omnibus. After NICE-3-knockdown with small interfering RNA in LUAD cells, cell proliferation was measured by cell counting,...
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Longxia Du Youru Wu Xiaodan Han Chen Wang Aili Li Guojin Huang Source Type: research

Interleukin-12 exacerbates symptoms in an MRL/MpJ-Faslpr mouse model of systemic lupus erythematosus
Exp Ther Med. 2021 Jun;21(6):627. doi: 10.3892/etm.2021.10059. Epub 2021 Apr 15.ABSTRACTInterleukin (IL)-12 modulates the generation and function of a variety of immune cells and serves an important role in the pathogenesis of autoimmune diseases. However, the precise role of IL-12 in the pathogenesis of systemic lupus erythematosus (SLE) remains to be elucidated. In the present study, the serum levels of IL-12 in patients with SLE were determined using an ELISA. The association between serum levels of IL-12 and clinical and laboratory indices, specifically, disease activity and complement 3, were analyzed. Recombinant IL-...
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Ling Li Xiaojun Sun Sisi Wu Xin Yuan Bingxin Liu Xueping Zhou Source Type: research

Simvastatin intervention mitigates hypercholesterolemia-induced alveolar bone resorption in rats
Exp Ther Med. 2021 Jun;21(6):628. doi: 10.3892/etm.2021.10060. Epub 2021 Apr 15.ABSTRACTSimvastatin promotes bone formation and increases bone mineral density in patients with hyperlipidemia and ameliorates hypercholesterolemia-induced microstructure changes in the jaw bone of animals. However, whether and how treatment with simvastatin can modulate the hypercholesterolemia-induced alveolar bone resorption is unclear. The present study aimed to examine the therapeutic efficacy and potential mechanisms of simvastatin application in hypercholesterolemia-induced alveolar bone resorption. The association between hyperlipidemia...
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Xiaoli Gao Jianhua Zhou Yuanyuan Bian Shengyun Huang Dongsheng Zhang Source Type: research

Thioredoxin relieves lipopolysaccharide-induced acute kidney injury in mice by reducing inflammation, oxidative stress and apoptosis
Exp Ther Med. 2021 Jun;21(6):629. doi: 10.3892/etm.2021.10061. Epub 2021 Apr 15.ABSTRACTAcute kidney injury (AKI) is a serious disease with rapid onset and a high mortality rate. It is therefore particularly important to identify a suitable method for treating AKI. Thioredoxin (Trx) is a potent anti-inflammatory and anti-oxidant protein that is prevalent in living organisms. The aim of the present study was to facilitate the clinical treatment of AKI via the study of Trx. Lipopolysaccharide (LPS) was used to construct an AKI model in mice and the mice were pre-treated with Trx to examine its effect on AKI. In addition, hum...
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Jingjing Wang Wenjuan Zhang Guoyuan Lu Source Type: research

miR-1184 regulates inflammatory responses and cell apoptosis by targeting TRADD in an LPS-induced cell model of sepsis
Exp Ther Med. 2021 Jun;21(6):630. doi: 10.3892/etm.2021.10062. Epub 2021 Apr 15.ABSTRACTMicroRNAs (miRs) have been reported to be potential clinical biomarkers for sepsis. miR-1184 is a multifunctional microRNA that exerts roles in the development of various diseases. However, the role of miR-1184 in children with sepsis remain unknown. In the present study, THP-1 cells were stimulated with 1 µg/ml lipopolysaccharide (LPS) for 24 h to establish an in vitro sepsis model. Reverse transcription-quantitative PCR was used to evaluate the expression of miR-1184 in clinical specimens, and of IL-6, TNF-α, IL-1β, m...
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Ping Ling Rong Tang Huazhu Wang Xiuqin Deng Jianli Chen Source Type: research

MicroRNA-325 inhibits the proliferation and induces the apoptosis of T cell acute lymphoblastic leukemia cells in a BAG2-dependent manner
In conclusion, the findings of the present study demonstrated that miR-325 directly targets the BAG2 gene and that the introduction of miR-325 can accelerate apoptosis and suppress the proliferation of Jurkat cells by silencing BAG2 expression.PMID:33936287 | PMC:PMC8082601 | DOI:10.3892/etm.2021.10063 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Fengyu Wang Fengli Wang Shengyu Zhang Xiaogang Xu Source Type: research

Interrelation of inflammation and oxidative stress in liver cirrhosis
Exp Ther Med. 2021 Jun;21(6):602. doi: 10.3892/etm.2021.10034. Epub 2021 Apr 14.ABSTRACTRecently, the trend of research has been focused on the role of hematological indicators in assessing the activities of various diseases. The aim of the present study was to determine the usefulness of such hematological indicators for assessment of the relationship between inflammation and oxidative stress in order to provide new predictive tools for a non-invasive investigation of disease outcome for liver cirrhosis patients. A total of 35 subjects with compensated or decompensated liver cirrhosis and 10 age-matched healthy volunteers...
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Mihnea Marian Pomacu Maria Diana Tra șcă Vlad P ădureanu Ana Maria Bug ă Ana Marina Andrei Elena Camelia St ănciulescu Ileana Monica Bani ță Dumitru R ădulescu C ătălina Gabriela Pisoschi Source Type: research

Catheter-related giant right atrial thrombosis mimicking a myxoma: A case report
In conclusion, differentiating intracardiac right atrial masses (RAMs) could may prove challenging. In our patient, clinical presentation and the preoperative investigations could not differentiate the right atrial thrombus from a myxoma, and only the postoperative histopathology diagnosis was able to guide correct diagnosis.PMID:33936260 | PMC:PMC8082616 | DOI:10.3892/etm.2021.10035 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Ovidiu Stiru Razvan Dragulescu Roxana Carmen Geana Andreea Chibulcutean Laura Raducu Adrian Tulin Andra Balcangiu Stroescu Camelia Diaconu Cornel Savu Nicolae Bacalbasa Irina Balescu Vlad Anton Iliescu Source Type: research

Manometric changes of the esophagus in morbidly obese patients
Exp Ther Med. 2021 Jun;21(6):604. doi: 10.3892/etm.2021.10036. Epub 2021 Apr 14.ABSTRACTThis prospective study aimed to determine the manometric pattern and the prevalence of esophageal dysmotility in 79 morbidly obese patients selected for laparoscopic sleeve gastrectomy. After clinical evaluation and upper gastrointestinal endoscopy, high-resolution esophageal manometry was performed. The esophageal peristalsis, lower esophageal sphincter (LES) basal pressure, and LES relaxation were evaluated. Demographic data showed a predominance of females (55.70%) and both females and males were in the 5th decade of life. In additio...
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Andrada L Popescu Raluca S Costache Daniel O Costache Vasile D Balaban Mariana Jinga Florentina Ionita-Radu Ana Caruntu Carmen Fierbinteanu-Braticevici Source Type: research

Predictors of the response to nivolumab immunotherapy in the second or subsequent lines for metastatic non-small cell lung cancers
Exp Ther Med. 2021 Jun;21(6):605. doi: 10.3892/etm.2021.10037. Epub 2021 Apr 14.ABSTRACTCheckpoint inhibitors represent the first therapeutic class to replace chemotherapy lines for the treatment of metastatic non-small cell lung cancer (NSCLC), due to improved overall survival and tolerability. Nivolumab, a fully human anti-programmed cell death-1 immunoglobulin G4 monoclonal antibody, is the first immune checkpoint inhibitor approved by the US Food and Drug Administration in 2014 for cases of metastatic melanoma and in 2015 for cases of squamous cell lung cancer and kidney cell cancer. The present study aimed to identify...
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Sorin S ăftescu Șerban Negru Simona Volov ăț Dorel Popovici Vlad Chercota Simona Stanca Horea Feier Daniel Malita Radu Dragomir Constantin Volov ăț Source Type: research

Fetal cardiac evaluation in HIV-positive women under HAART therapy in a Romanian hospital
Exp Ther Med. 2021 Jun;21(6):606. doi: 10.3892/etm.2021.10038. Epub 2021 Apr 14.ABSTRACTHuman immunodeficiency virus (HIV) is an RNA (ribonucleic acid) virus that causes acquired immunodeficiency syndrome (AIDS). This disease has a major socioeconomical impact. The aim of the present study was to assess the fetal heart structure and function in HIV-positive pregnant women on highly active antiretroviral therapy (HAART). This is a prospective study conducted between January, 2018 and December, 2019. The hearts of fetuses from 14 HIV-positive pregnant women on HAART were assessed. Statistically significant sex-related cardia...
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Alexandra Ursache Alexandra Maria Tibeica Alexandru Luca Mircea Onofriescu Daniela Roxana Matasariu Dragos Nemescu Source Type: research

Incidence of thyroid carcinomas in an extended retrospective study of 526 autopsies
Exp Ther Med. 2021 Jun;21(6):607. doi: 10.3892/etm.2021.10039. Epub 2021 Apr 14.ABSTRACTThyroid cancer accounts for 1% of all malignancies, and is becoming increasingly common worldwide. The literature reports a prevalence of ~50% of thyroid nodules detected during autopsies in subjects with unknown thyroid pathology. An extended retrospective study of 526 autopsy cases was performed to identify the prevalence of thyroid carcinoma, among various types of thyroid nodules identified incidentally. Tissue samples were taken from thyroid nodules, for investigation of the presence of thyroid carcinoma, along with their macroscop...
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Iuliana Sobaru Mohorea Bogdan Socea Drago ş Şerban Zenaida Ceausu Adrian Tulin Violeta Melinte Mihai Ceausu Source Type: research

Crosstalk between vitamin D axis, inflammation and host immunity mechanisms: A prospective study
Exp Ther Med. 2021 Jun;21(6):608. doi: 10.3892/etm.2021.10040. Epub 2021 Apr 14.ABSTRACTTuberculosis (TB) remains a public health burden, after many years at attempts for its eradication. Vitamin D (VD) status has been suggested to be related to TB susceptibility because it has the ability to regulate multiple axes of the innate and adaptive host immune response. VD mediates cathelicidin (LL-37) synthesis, a cationic bactericidal peptide, through the expression of vitamin D receptor (VDR). Host innate defense mechanisms include autophagy and apoptosis of alveolar macrophages. The present study aimed to assess the relations...
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Andreea-Daniela Meca Simona Ștefănescu Maria Bogdan Adina Turcu- Știolică Floarea Mimi Ni țu Marius Matei Ramona Cioboat ă Ana Maria Bug ă C ătălina-Gabriela Pisoschi Source Type: research

Respiratory allergies: Salicaceae sensitization (Review)
Exp Ther Med. 2021 Jun;21(6):609. doi: 10.3892/etm.2021.10041. Epub 2021 Apr 14.ABSTRACTSeasonal allergic rhinitis (SAR) is one of the most frequent chronic conditions of the modern world. Pollen carried by the wind from pollinated trees is a major source of SAR. Betulaceae, Oleaceae and Platanus are the most important sources of airway sensitization with regard to tree pollen and, therefore, they are included in the official recommendations of skin prick testing by different official societies. Salicaceae pollen is a moderate source of pollen sensitization. Conversely, large areas are covered with poplars and willows arou...
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Adrian Costache Ovidiu Nicolae Berghi Romica Cergan Mihai Dumitru Adriana Neagos Liliana Gabriela Popa Calin Giurcaneanu Daniela Vrinceanu Source Type: research

Muscular dystrophy: Experimental animal models and therapeutic approaches (Review)
Exp Ther Med. 2021 Jun;21(6):610. doi: 10.3892/etm.2021.10042. Epub 2021 Apr 14.ABSTRACTThe muscular dystrophies are a heterogeneous group of genetically inherited diseases characterized by muscle weakness and progressive wasting, which can cause premature death in severe forms. Although>30 years have passed since the identification of the first protein involved in a type of muscular dystrophy, there is no effective treatment for these disabling disorders. In the last decade, several novel therapeutic approaches have been developed and investigated as promising therapeutic approaches aimed to ameliorate the dystrophic p...
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Gisela Gaina Alexandra Popa Gruianu Source Type: research

Ultrasonography used in the diagnosis of chronic rhinosinusitis: From experimental imaging to clinical practice
Exp Ther Med. 2021 Jun;21(6):611. doi: 10.3892/etm.2021.10043. Epub 2021 Apr 14.ABSTRACTThere is an increasing incidence of sinusitis cases in outpatient clinics; therefore, new methods for screening and triage are required. Performance of sinus sonography in our outpatient protocol was assessed in order to ascertain the specificity and sensitivity of this imaging method to detect rhinosinusitis. A total of 81 consecutive cases with clinical signs of sinusitis were collected and clinical data compared with sinus sonography results. Moreover, sinus sonography enhanced referral for further computed tomography scans. The resu...
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Adriana Neagos Mihai Dumitru Daniela Vrinceanu Adrian Costache Andreea Nicoleta Marinescu Romica Cergan Source Type: research

Prevalence of < em > GJB2 < /em > gene mutations correlated to presence of clinical and environmental risk factors in the etiology of congenital sensorineural hearing loss of the Romanian population
Exp Ther Med. 2021 Jun;21(6):612. doi: 10.3892/etm.2021.10044. Epub 2021 Apr 14.ABSTRACTAlthough etiologically heterogeneous at least 50% of all early on-set hearing losses have a genetic cause and of these, the large majority, 75-80% are most probably autosomal recessive and 70% are non-syndromic. The rest of the congenital hearing losses are determined by clinical and environmental factors such as ototoxic medication, prematurity, and complications at birth. During the last decade it became clear that 50-80% of all such afflictions result from mutations in a single gene, GJB2, which encodes the protein Connexin 26. In or...
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Alexandra Neagu Adela-Ioana Mocanu Alexandru Bonciu Gabriella Coad ă Horia Mocanu Source Type: research

PHLDA3 inhibition attenuates endoplasmic reticulum stress-induced apoptosis in myocardial hypoxia/reoxygenation injury by activating the PI3K/AKT signaling pathway
In conclusion, in the process of H/R injury, PHLDA3 inhibition reduced ERS-induced apoptosis and H/R injury by activating the PI3K/AKT pathway. PHLDA3 may be a therapeutic target for the treatment of MIRI.PMID:33936270 | PMC:PMC8082641 | DOI:10.3892/etm.2021.10045 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Kai Liu Ying Chen Fen Ai Yun-Qian Li Kun Zhang Wei-Tong Zhang Source Type: research

Novel ARID1B variant inherited from somatogonadal mosaic mother in siblings with Coffin-Siris syndrome 1
Exp Ther Med. 2021 Jun;21(6):614. doi: 10.3892/etm.2021.10046. Epub 2021 Apr 14.ABSTRACTCoffin-Siris syndrome1 (CSS1; Online Mendelian Inheritance in Man no. 135900) is a multiple malformation syndrome characterized by intellectual and/or developmental delay, and hypoplastic or absent fifth fingernails and/or toenails. AT-rich interaction domain-containing protein 1B (ARID1B) is the most frequently mutated gene in CSS1 and the majority of reported cases have been sporadic. Using whole-exome sequencing, the present study identified two siblings with CSS1 with a novel heterozygous co-segregating pathogenic variant in the ARI...
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Zhong Min Cheng Qian Dai Ying Source Type: research

Exogenous transforming growth factor- β1 prevents the inflow of fluoride to ameleoblasts through regulation of voltage-gated chloride channels 5 and 7
Exp Ther Med. 2021 Jun;21(6):615. doi: 10.3892/etm.2021.10047. Epub 2021 Apr 14.ABSTRACTDental fluorosis is a global issue. Although there are multiple causes of dental fluorosis, the precise mechanism remains controversial. Previous studies have demonstrated that extracellular fluoride may promote an accumulation of fluoride ions in ameloblasts, which may induce oxidative and endoplasmic reticulum stresses, leading to dental fluorosis. However, the exact process by which fluoride ions enter cells has not been determined. In the present study, intracellular fluoride concentration was determined using a newly developed spec...
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Mei Ji Xuejing Duan Xiaohui Han Jing Sun Dongsheng Zhang Source Type: research

ΜicroRNA-122 protects against ischemic stroke by targeting Maf1
In conclusion, the results suggested that miR-122 improved the outcome of acute ischemic stroke by reducing the expression of Maf1.PMID:33936273 | PMC:PMC8082594 | DOI:10.3892/etm.2021.10048 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Mengmeng Wang Xiaoman Liu Yu Wu Yi Wang Jiahui Cui Jing Sun Ying Bai Ming-Fei Lang Source Type: research

Changes in bone mineral density, 25-hydroxyvitamin D < sup > 3 < /sup > and inflammatory factors in patients with hyperthyroidism
Exp Ther Med. 2021 Jun;21(6):617. doi: 10.3892/etm.2021.10049. Epub 2021 Apr 14.ABSTRACTThe present study aimed to evaluate changes in bone mineral density, 25-hydroxyvitamin D3 [25-(OH)D3] and inflammatory factors in patients with hyperthyroidism, in order to determine the correlations with the pathogenesis of hyperthyroidism. A total of 55 patients with hyperthyroidism (observation group) and 53 healthy patients (control group) enrolled at Weifang People's Hospital from March 2017 to February 2018 were randomly enrolled. The thyroid function, bone mineral density, 25-(OH)D3 and inflammatory factors were measured and comp...
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Yali Zhou Xixia Wang Maoyuan Xin Haiting Zhuang Source Type: research

Total flavonoids of < em > Rhizoma Drynariae < /em > combined with calcium attenuate osteoporosis by reducing reactive oxygen species generation
Exp Ther Med. 2021 Jun;21(6):618. doi: 10.3892/etm.2021.10050. Epub 2021 Apr 14.ABSTRACTIn the present study, the effects of total flavonoids of Rhizoma Drynariae (TFRD) and calcium carbonate (CaCO3) on osteoporosis (OP) were assessed in a rat model of OP. For this purpose, 36 Sprague-Dawley rats, aged 3 months, were randomly divided into a group undergoing sham surgery (sham-operated group), model group (OP group), CaCO3 group (OP + CaCO3 group), TFRD group (OP + TFRD group), TFRD combined with CaCO3 group (OP + TFRD + CaCO3 group) and TFRD and CaCO3 combined with N-acetyl cysteine group (OP + TFRD + CaCO3 + NAC group). T...
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Panyun Mu Yimei Hu Xu Ma Jingru Shi Zhendong Zhong Lingyuan Huang Source Type: research

Polycomb group protein Bmi1 is required for the neuronal differentiation of mouse induced pluripotent stem cells
Exp Ther Med. 2021 Jun;21(6):619. doi: 10.3892/etm.2021.10051. Epub 2021 Apr 14.ABSTRACTInduced pluripotent stem cells (iPSCs) reprogrammed by somatic cells may be used as a potentially novel treatment regimen in stem cell regenerative medicine, particularly in the central nervous system (CNS). In the present study, iPSCs were generated using mouse embryonic fibroblasts by ectopic overexpression of Sox-2, Oct-3/4, Klf-4 and c-Myc, and cultured under the same conditions as that used for embryonic stem cells. The neuronal differentiation capacity of mouse iPSCs was examined, and the involvement of the formation of embryoid ...
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Wei Shan Liping Zhou Lizhen Liu Deju Lin Qin Yu Source Type: research

Transforming growth factor- β1-induced podocyte injury is associated with increased microRNA-155 expression, enhanced inflammatory responses and MAPK pathway activation
Exp Ther Med. 2021 Jun;21(6):620. doi: 10.3892/etm.2021.10052. Epub 2021 Apr 14.ABSTRACTMicroRNA-155 (miR-155) is associated with various diseases. However, the potential role of miR-155 in early glomerular disease (EGD) remains elusive. In the present study, the clinical significance of urinary miR-155 expression was explored in patients with EGD using receiver operating characteristic curve analysis. Conditionally immortalized mouse podocytes were cultured in vitro and treated with transforming growth factor-β1 (TGF-β1) at different concentrations and durations. The gene expression levels of mRNAs and miR-155 w...
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Xintong Zheng Qiuhong Zhong Xu Lin Xianjun Gu Xiaoyan Ling Zhao Liang Qing Qin Xiuri Du Source Type: research

Joint line plate fixation for tibial plateau fractures caused by hyperextension varus
In conclusion, internal fixation with a joint line plate is an appropriate treatment for tibial plateau fractures involving the anteromedial margin with good clinical efficacy.PMID:33936278 | PMC:PMC8082576 | DOI:10.3892/etm.2021.10053 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Yu-Cheng Huang Jing Jiao Wen-Jun Cheng Fei Xiao Wei Zuo Jun-Wen Wang Source Type: research

miR-491-5p inhibits the proliferation and migration of A549 cells by FOXP4
Exp Ther Med. 2021 Jun;21(6):622. doi: 10.3892/etm.2021.10054. Epub 2021 Apr 14.ABSTRACTAberrant expression of microRNAs (miRNAs/miRs) plays a key role in the development of non-small cell lung cancer (NSCLC). In the present study, lower miRNA (miR)-491-5p levels and a higher forkhead box P4 (FOXP4) mRNA level were observed in NSCLC tissues and cell lines, compared to adjacent tissues and the normal human lung epithelial cell line BEAS-2B, respectively. A549 cell proliferation and migration were inhibited upon transfection of miR-491-5p mimics compared to miR-negative control (NC) mimics. In addition, compared to miR-NC mi...
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Fuyong Wu Aiping Ji Zhenkun Zhang Jinfang Li Penglong Li Source Type: research

Effect of media distraction (audio-visual and music) for pain and anxiety control in patients undergoing shock-wave lithotripsy: A systematic review and meta-analysis
Exp Ther Med. 2021 Jun;21(6):623. doi: 10.3892/etm.2021.10055. Epub 2021 Apr 14.ABSTRACTAudio-visual (AV) or music distraction may be used to reduce pain during several healthcare procedures. The present manuscript is a systematic review and meta-analysis to assess the effectiveness of media distraction in reducing pain and anxiety in extracorporeal shock wave lithotripsy (ESWL) patients. The PubMed, Embase, Scopus, BioMed Central, Ovoid and CENTRAL (Cochrane Central Register of Controlled Trials) databases were screened for studies assessing the role of media distraction (music/AV media) in reducing pain and anxiety of ES...
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Weihua Hu Ke Yang Li Zhang Xu Lu Source Type: research

Chemical hemorrhagic cystitis: Diagnostic and therapeutic pitfalls (Review)
In conclusion, CC is a disease with an increasing incidence, challenging to diagnose, which is frequently mistreated, and has multiple treatment modalities that still require standardization in administration and sequencing.PMID:33936281 | PMC:PMC8082586 | DOI:10.3892/etm.2021.10056 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Razvan-Cosmin Petca Razvan-Ionut Popescu Cristian Toma Mihai Cristian Dumitrascu Aida Petca Florica Sandru Calin Bogdan Chibelean Source Type: research

NICE-3-knockdown induces cell cycle arrest and autophagy in lung adenocarcinoma cells via the AKT/mTORC1 signaling pathway
Exp Ther Med. 2021 Jun;21(6):625. doi: 10.3892/etm.2021.10057. Epub 2021 Apr 15.ABSTRACTThe NICE-3 protein serves an oncogenic role in hepatocellular carcinoma, but its role in lung adenocarcinoma (LUAD) remains unknown. The aim of the present study was to investigate the potential role and underlying mechanisms of NICE-3 in LUAD. In the present study, NICE-3 expression in LUAD tissues and its association with patient prognosis were analyzed using datasets from The Cancer Genome Atlas and Gene Express Omnibus. After NICE-3-knockdown with small interfering RNA in LUAD cells, cell proliferation was measured by cell counting,...
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Longxia Du Youru Wu Xiaodan Han Chen Wang Aili Li Guojin Huang Source Type: research

Interleukin-12 exacerbates symptoms in an MRL/MpJ-Faslpr mouse model of systemic lupus erythematosus
Exp Ther Med. 2021 Jun;21(6):627. doi: 10.3892/etm.2021.10059. Epub 2021 Apr 15.ABSTRACTInterleukin (IL)-12 modulates the generation and function of a variety of immune cells and serves an important role in the pathogenesis of autoimmune diseases. However, the precise role of IL-12 in the pathogenesis of systemic lupus erythematosus (SLE) remains to be elucidated. In the present study, the serum levels of IL-12 in patients with SLE were determined using an ELISA. The association between serum levels of IL-12 and clinical and laboratory indices, specifically, disease activity and complement 3, were analyzed. Recombinant IL-...
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Ling Li Xiaojun Sun Sisi Wu Xin Yuan Bingxin Liu Xueping Zhou Source Type: research

Simvastatin intervention mitigates hypercholesterolemia-induced alveolar bone resorption in rats
Exp Ther Med. 2021 Jun;21(6):628. doi: 10.3892/etm.2021.10060. Epub 2021 Apr 15.ABSTRACTSimvastatin promotes bone formation and increases bone mineral density in patients with hyperlipidemia and ameliorates hypercholesterolemia-induced microstructure changes in the jaw bone of animals. However, whether and how treatment with simvastatin can modulate the hypercholesterolemia-induced alveolar bone resorption is unclear. The present study aimed to examine the therapeutic efficacy and potential mechanisms of simvastatin application in hypercholesterolemia-induced alveolar bone resorption. The association between hyperlipidemia...
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Xiaoli Gao Jianhua Zhou Yuanyuan Bian Shengyun Huang Dongsheng Zhang Source Type: research

Thioredoxin relieves lipopolysaccharide-induced acute kidney injury in mice by reducing inflammation, oxidative stress and apoptosis
Exp Ther Med. 2021 Jun;21(6):629. doi: 10.3892/etm.2021.10061. Epub 2021 Apr 15.ABSTRACTAcute kidney injury (AKI) is a serious disease with rapid onset and a high mortality rate. It is therefore particularly important to identify a suitable method for treating AKI. Thioredoxin (Trx) is a potent anti-inflammatory and anti-oxidant protein that is prevalent in living organisms. The aim of the present study was to facilitate the clinical treatment of AKI via the study of Trx. Lipopolysaccharide (LPS) was used to construct an AKI model in mice and the mice were pre-treated with Trx to examine its effect on AKI. In addition, hum...
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Jingjing Wang Wenjuan Zhang Guoyuan Lu Source Type: research

miR-1184 regulates inflammatory responses and cell apoptosis by targeting TRADD in an LPS-induced cell model of sepsis
Exp Ther Med. 2021 Jun;21(6):630. doi: 10.3892/etm.2021.10062. Epub 2021 Apr 15.ABSTRACTMicroRNAs (miRs) have been reported to be potential clinical biomarkers for sepsis. miR-1184 is a multifunctional microRNA that exerts roles in the development of various diseases. However, the role of miR-1184 in children with sepsis remain unknown. In the present study, THP-1 cells were stimulated with 1 µg/ml lipopolysaccharide (LPS) for 24 h to establish an in vitro sepsis model. Reverse transcription-quantitative PCR was used to evaluate the expression of miR-1184 in clinical specimens, and of IL-6, TNF-α, IL-1β, m...
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Ping Ling Rong Tang Huazhu Wang Xiuqin Deng Jianli Chen Source Type: research

MicroRNA-325 inhibits the proliferation and induces the apoptosis of T cell acute lymphoblastic leukemia cells in a BAG2-dependent manner
In conclusion, the findings of the present study demonstrated that miR-325 directly targets the BAG2 gene and that the introduction of miR-325 can accelerate apoptosis and suppress the proliferation of Jurkat cells by silencing BAG2 expression.PMID:33936287 | PMC:PMC8082601 | DOI:10.3892/etm.2021.10063 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - May 3, 2021 Category: General Medicine Authors: Fengyu Wang Fengli Wang Shengyu Zhang Xiaogang Xu Source Type: research

Paeoniflorin suppresses allergic and inflammatory responses by promoting autophagy in rats with urticaria
In conclusion, PF could effectively improve urticarial lesions by inhibiting inflammatory cytokine IL-23 and increasing the autophagic activity via the LKB1/AMPK-α pathway.PMID:33884028 | PMC:PMC8056118 | DOI:10.3892/etm.2021.10022 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - April 22, 2021 Category: General Medicine Authors: Jing Guo Li Peng Jinhao Zeng Meiheng Zhang Feng Xu Xiaotong Zhang Qin Wei Source Type: research

Comparison of anti-inflammatory effects of Mecasin and its constituents on lipopolysaccharide-stimulated BV2 cells
Exp Ther Med. 2021 Jun;21(6):591. doi: 10.3892/etm.2021.10023. Epub 2021 Apr 8.ABSTRACTMecasin, a traditional medicine, contains nine herbal constituents: Curcuma longa, Salvia miltio rhiza, Gastrodia elata, Chaenomeles sinensis, Polygala tenuifolia, Paeonia japonica, Glycyrrhiza uralensis, Atractylodes japonica and processed Aconitum carmichaeli. Several biological effects of mecasin have been described both in vivo and in vitro. Previous studies have demonstrated that mecasin has anti-inflammatory effects. The purpose of the present study was to determine anti-inflammatory effects of mecasin and its natural product const...
Source: Experimental and Therapeutic Medicine - April 22, 2021 Category: General Medicine Authors: Tingting Wang Wonmin Ko Joon-Yeong Shin Dongho Choi Dong-Sung Lee Sungchul Kim Source Type: research

Downregulation of miR-588 is associated with tumor progression and unfavorable prognosis in patients with osteosarcoma
Exp Ther Med. 2021 Jun;21(6):592. doi: 10.3892/etm.2021.10024. Epub 2021 Apr 8.ABSTRACTOsteosarcoma (OS) is a primary malignant tumor characterized by a high metastatic potential and poor prognosis. The dysregulation of miR-588 has been demonstrated to serve crucial roles in the progression of numerous types of cancer. The present study aimed to investigate the expression and function of miR-588 in the development of OS. To do so, clinical samples were collected and analyzed, and in vitro experiments were conducted. A total of 104 patients with OS were recruited between 2012 and 2014. The expression of miR-588 was analyzed...
Source: Experimental and Therapeutic Medicine - April 22, 2021 Category: General Medicine Authors: Tao Yu Shuang Liang Tianhong Ma Weidong Song Source Type: research