Diabetic neuropathic pain induced by streptozotocin alters the expression profile of non-coding RNAs in the spinal cord of mice as determined by sequencing analysis
Exp Ther Med. 2021 Jul;22(1):775. doi: 10.3892/etm.2021.10207. Epub 2021 May 18.ABSTRACTDiabetic neuropathic pain (DNP) is one of the most serious complications of diabetes. Patients with DNP always exhibit spontaneous and stimulus-evoked pain. However, the pathogenesis of DNP remains to be fully elucidated. Non-coding RNAs (ncRNAs) serve important roles in several cellular processes and dysregulated expression may result in the development of several diseases, including DNP. Although ncRNAs have been suggested to be involved in the pathogenesis of DNP, their precise roles remain to be determined. In the present study, seq...
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Jian He Han Bin Wang Jiang Ju Huang Lei Zhang Dong Lin Li Wan You He Qing Ming Xiong Zai Sheng Qin Source Type: research

CLCA2 suppresses the proliferation, migration and invasion of cervical cancer
In conclusion, the results of the present study demonstrated that CLCA2 suppressed the proliferation, migration and invasion of cervical cancer cells, and that CLCA2 may be a potential therapeutic target of cervical cancer.PMID:34055075 | PMC:PMC8145432 | DOI:10.3892/etm.2021.10208 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Peijin Zhang Yang Lin Yaqiong Liu Source Type: research

Analysis of the effects of prepared porcelain veneers and unprepared porcelain veneers on gingival crevicular flora based on high-throughput sequencing
Exp Ther Med. 2021 Jul;22(1):777. doi: 10.3892/etm.2021.10209. Epub 2021 May 18.ABSTRACTThe effect of tooth preparation on the gingival crevicular flora of abutment teeth during porcelain veneer treatment is not clear. The purpose of the present study was to analyze the difference between prepared porcelain veneers and unprepared porcelain veneers on gingival crevicular flora. High-throughput sequencing was used in the present study. A total of 20 patients (40 anterior teeth) with veneer restoration of anterior teeth were enrolled. They were divided into two groups: The prepared porcelain veneer group (group P, 11 cases, 1...
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Rui Zhang Lanhua Shen Dandan Xu Xiao Li Source Type: research

rs1344706 polymorphism of zinc finger protein 804a (ZNF804a) gene related to the integrity of white matter fiber bundle in schizophrenics
Exp Ther Med. 2021 Jul;22(1):778. doi: 10.3892/etm.2021.10210. Epub 2021 May 18.ABSTRACTGenetic factors play an important role in the pathogenesis of schizophrenia (SZ), and the zinc finger protein 804a (ZNF804a) gene has been considered to be a risk gene for schizophrenia. In the present study, the correlation between rs1344706 polymorphism of ZNF804a gene and the integrity of white matter in schizophrenic cases was explored. A total of 60 SZ patients and 100 healthy controls (HC) were included to undergo head MRI. According to the genotyping of rs1344706 in ZNF804a, the subjects in each group were divided into a normal a...
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Jian Zhou Quan Bao Shuang Liang Hong Guo Xin Meng Guangfeng Zhang Ping Li Source Type: research

Expression and prognostic value of SULT1A2 in bladder cancer
Exp Ther Med. 2021 Jul;22(1):779. doi: 10.3892/etm.2021.10211. Epub 2021 May 19.ABSTRACTSulfotransferase Family 1A Member 2 (SULT1A2) is a protein coding gene. Several studies have reported that SULT1A2 may have a chemical carcinogenic effect if expressed as a functional protein. The present study aimed to investigate the expression and potential role of SULT1A2 in bladder cancer (BC). Data from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus databases were used to analyze SULT1A2 expression in BC. In addition, reverse transcription-quantitative PCR and western blot analyses were performed to detect SULT1A2 expr...
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Yinghui Chao Qifeng Ou Jin Shang Source Type: research

miR-3677-5p promotes the proliferation, migration and invasion of hepatocellular carcinoma cells and is associated with prognosis
In conclusion, the present study demonstrated that miR-3677-5p acts as an oncogene that has a critical role in the regulation of HCC proliferation and progression. Hence, miR-3677-5p may serve as a valuable prognostic biomarker and may be developed as a promising therapeutic target for HCC.PMID:34055079 | PMC:PMC8145920 | DOI:10.3892/etm.2021.10212 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Hai-Xiang Mao Bai-Wen Chen Jie Wang Chen-Yang Ma Yi-Chao Gan Kai-Jie Qiu Source Type: research

Long non-coding RNA Rian protects against experimental bronchopulmonary dysplasia by sponging miR-421
Exp Ther Med. 2021 Jul;22(1):781. doi: 10.3892/etm.2021.10213. Epub 2021 May 19.ABSTRACTBronchopulmonary dysplasia (BPD) is a frequent complication characterized by accelerated lung alveolarization in newborns. Long non-coding RNAs (lncRNAs) and microRNAs (miRs) are regarded as essential regulators in various diseases, including BPD. However, the detailed mechanism of the functions of RNA imprinted and accumulated in nucleus (Rian) lncRNA in the progression of BPD have remained elusive. The aim of the present study was to illustrate the interaction between miR-421 and Rian in BPD models and MLE-12 cells. The ability of Ria...
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Xifeng Tao Yafei Fang Chen Huo Source Type: research

Ginsenoside Rg1 alleviates lipopolysaccharide-induced neuronal damage by inhibiting NLRP1 inflammasomes in HT22 cells
Exp Ther Med. 2021 Jul;22(1):782. doi: 10.3892/etm.2021.10214. Epub 2021 May 19.ABSTRACTLipopolysaccharide (LPS) is a toxic component of cell walls of Gram-negative bacteria that are widely present in gastrointestinal tracts. Increasing evidence showed that LPS plays important roles in the pathogeneses of neurodegenerative disorders, such as Alzheimer's disease (AD). NADPH oxidase s2 (NOX2) is a complex membrane protein that contributes to the production of reactive oxygen species (ROS) in several neurological diseases. The NLRP1 inflammasome can be activated in response to an accumulation of ROS in neurons. However, it is...
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Yaodong Zhang Shixin Ding Yali Chen Zhenghao Sun Junyan Zhang Yuli Han Xianan Dong Zhirui Fang Weizu Li Source Type: research

Curcumin ameliorates ischemic stroke injury in rats by protecting the integrity of the blood-brain barrier
Exp Ther Med. 2021 Jul;22(1):783. doi: 10.3892/etm.2021.10215. Epub 2021 May 19.ABSTRACTThe blood-brain barrier (BBB) is critical for proper cerebral homeostasis and its dysfunction during ischemic stroke can result in significant neurological injury. The major goal of the present study was to identify whether curcumin pretreatment possessed protective effects on BBB integrity during the 24 h of acute ischemic brain injury. To investigate the protective effects of curcumin, male Sprague-Dawley rats were divided into multiple groups, including sham, middle cerebral artery occlusion/reperfusion (MCAO/R) vehicle and curcumin ...
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Shuguang Wu Ting Guo Wenxuan Qi Yuyu Li Jie Gu Cui Liu Yuehong Sha Baocheng Yang Shuqun Hu Xuemei Zong Source Type: research

Bioinformatics identification of hub genes and signaling pathways regulated by intravenous immunoglobulin treatment in acute Kawasaki disease
Exp Ther Med. 2021 Jul;22(1):784. doi: 10.3892/etm.2021.10216. Epub 2021 May 19.ABSTRACTKawasaki disease (KD) is an acute, self-limiting form of vasculitis commonly encountered in infants and young children. Intravenous immunoglobulin (IVIG) is the primary drug used for the treatment of KD, which may significantly reduce the occurrence of coronary artery lesions. However, the specific molecular profile changes of KD caused by IVIG treatment have remained elusive and require further research. The present study was designed to identify key genes, pathways and immune cells affected by IVIG treatment using multiple bioinformat...
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Hongbiao Huang Lei Xu Yueyue Ding Jie Qin Chengcheng Huang Xuan Li Yunjia Tang Guanghui Qian Haitao Lv Source Type: research

Etomidate attenuates hyperoxia-induced acute lung injury in mice by modulating the Nrf2/HO-1 signaling pathway
Exp Ther Med. 2021 Jul;22(1):785. doi: 10.3892/etm.2021.10217. Epub 2021 May 19.ABSTRACTThe present study aimed to investigate the protective effects of etomidate on hyperoxia-induced acute lung injury in mice, particularly on the nuclear factor-erythroid 2-related factor 2 (Nrf2)/heme oxygenase 1 (HO-1) pathway. Fifty specific pathogen-free mice were randomly divided into the blank control, model, high oxygen exposure + low etomidate dose (0.3 mg·kg-1), a high oxygen exposure + moderate etomidate dose (3 mg·kg-1), and a high oxygen exposure + high etomidate dose (10 mg·kg-1) groups, with ten mice allo...
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Liming Jia Hongzhong Hao Chunyu Wang Jianfeng Wei Source Type: research

Comparison of modified corneal cross-linking with intrastromal voriconazole for the treatment of fungal corneal ulcer
In conclusion, the present study suggested that CXL could have a beneficial impact for treating fungal corneal ulcers in the aspects of infection control, localized lesions and accelerated epithelialization. In addition, except the loss of corneal transparency, this treatment approach could be applied with reduced risks of adverse events.PMID:34055085 | PMC:PMC8145909 | DOI:10.3892/etm.2021.10218 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Yingxin Chen Xingya Miao Minghong Gao Lixin Song Source Type: research

Modulated electro-hyperthermia with weekly paclitaxel or cisplatin in patients with recurrent or persistent epithelial ovarian, fallopian tube or primary peritoneal carcinoma: The KGOG 3030 trial
In conclusion, mEHT therapy with weekly paclitaxel or cisplatin appeared safe and warrants further investigation. The present trial was registered with www.clinicaltrials.gov on January 22, 2015 (trial registration no. NCT02344095).PMID:34055086 | PMC:PMC8145814 | DOI:10.3892/etm.2021.10219 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Kidong Kim Jae-Hoon Kim Seung Cheol Kim Yong Beom Kim Byung-Ho Nam Jae Hong No Hanbyoul Cho Woong Ju Dong Hoon Suh Yun Hwan Kim Source Type: research

Oncogenic role of epithelial cell transforming sequence 2 in lung adenocarcinoma cells
Exp Ther Med. 2021 Jul;22(1):788. doi: 10.3892/etm.2021.10220. Epub 2021 May 21.ABSTRACT[This retracts the article DOI: 10.3892/etm.2016.3584.].PMID:34055087 | PMC:PMC8145908 | DOI:10.3892/etm.2021.10220 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Hongyi Tan Xiaoshan Wang Xiaogang Yang Haitao Li Ben Liu Pinhua Pan Source Type: research

Combination of endothelial progenitor cells and BB-94 significantly alleviates brain damage in a mouse model of diabetic ischemic stroke
Exp Ther Med. 2021 Jul;22(1):789. doi: 10.3892/etm.2021.10221. Epub 2021 May 21.ABSTRACTIschemic stroke is a complication of chronic macrovascular disease in type 2 diabetes. However, the pathogenesis of diabetic ischemic stroke has not yet been fully clarified. The aim of the present study was to investigate the underlying effects of endothelial progenitor cells (EPCs) and the matrix metalloproteinase inhibitor BB-94 on diabetic stroke. In vitro experiments were performed using oxygen-glucose deprivation/reoxygenation (OGD/R) model cells, established using HT22 mouse hippocampal cells. MTT assays and flow cytometry reveal...
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Daixuan Zhou Zhi Huang Xiaoxi Zhu Tao Hong Yuanli Zhao Source Type: research

Anti-inflammatory role of curcumin in retinal disorders (Review)
Exp Ther Med. 2021 Jul;22(1):790. doi: 10.3892/etm.2021.10222. Epub 2021 May 21.ABSTRACTCurcumin [1,7-bis-(4-hydroxy-3-methoxyphenyl)-hepta-1,6-diene-3,5-dione], the main component of turmeric (Curcuma longa, a flowering plant of the ginger family, Zingiberaceae), is known to possess different pharmacological activities, particularly anti-inflammatory and antioxidant properties. Since an underlying inflammatory process exists in several ocular conditions, such as anterior uveitis, glaucoma, age-related macular degeneration (AMD) and diabetic retinopathy (DR), the aim of the present review was to summarize the pleiotropic e...
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Federica Franzone Marcella Nebbioso Tiziano Pergolizzi Giuseppe Attanasio Angela Musacchio Antonio Greco Paolo Giuseppe Limoli Marco Artico Demetrios A Spandidos Samanta Taurone Enzo Agostinelli Source Type: research

Effects of NSAIDs on pre-osteoblast viability and osteogenic differentiation
Exp Ther Med. 2021 Jul;22(1):740. doi: 10.3892/etm.2021.10172. Epub 2021 May 10.ABSTRACTNon-steroidal anti-inflammatory drugs (NSAIDs) are widely used in the treatment of a variety of musculoskeletal conditions, injuries and after surgery for postoperative pain management. Their use has been associated with impaired bone healing, possibly due to a multifactorial function, which may include inhibition of osteoblast recruitment and differentiation. However, up to date, there is no consensus regarding the impact of NSAIDs on bone-healing. The aim of the current study was to investigate the effects of five NSAIDs on the cellul...
Source: Experimental and Therapeutic Medicine - May 28, 2021 Category: General Medicine Authors: Chrystalleni Hadjicharalambous Kalliopi Alpantaki Maria Chatzinikolaidou Source Type: research

Proteomics reveals urine apolipoprotein A-I as a potential biomarker of acute kidney injury following percutaneous coronary intervention in elderly patients
In conclusion, apoA-I may be a promising biomarker for the early diagnosis of PCI-AKI in elderly patients. The role of apoA-I in the pathobiology of PCI-AKI requires further exploration.PMID:34046095 | PMC:PMC8141962 | DOI:10.3892/etm.2021.10177 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - May 28, 2021 Category: General Medicine Authors: Fangfang Zhou Qun Luo Lina Han Gen Shen Lulu Huang Honghua Ye Source Type: research

Effects of NSAIDs on pre-osteoblast viability and osteogenic differentiation
Exp Ther Med. 2021 Jul;22(1):740. doi: 10.3892/etm.2021.10172. Epub 2021 May 10.ABSTRACTNon-steroidal anti-inflammatory drugs (NSAIDs) are widely used in the treatment of a variety of musculoskeletal conditions, injuries and after surgery for postoperative pain management. Their use has been associated with impaired bone healing, possibly due to a multifactorial function, which may include inhibition of osteoblast recruitment and differentiation. However, up to date, there is no consensus regarding the impact of NSAIDs on bone-healing. The aim of the current study was to investigate the effects of five NSAIDs on the cellul...
Source: Experimental and Therapeutic Medicine - May 28, 2021 Category: General Medicine Authors: Chrystalleni Hadjicharalambous Kalliopi Alpantaki Maria Chatzinikolaidou Source Type: research

Proteomics reveals urine apolipoprotein A-I as a potential biomarker of acute kidney injury following percutaneous coronary intervention in elderly patients
In conclusion, apoA-I may be a promising biomarker for the early diagnosis of PCI-AKI in elderly patients. The role of apoA-I in the pathobiology of PCI-AKI requires further exploration.PMID:34046095 | PMC:PMC8141962 | DOI:10.3892/etm.2021.10177 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - May 28, 2021 Category: General Medicine Authors: Fangfang Zhou Qun Luo Lina Han Gen Shen Lulu Huang Honghua Ye Source Type: research

Effects of NSAIDs on pre-osteoblast viability and osteogenic differentiation
Exp Ther Med. 2021 Jul;22(1):740. doi: 10.3892/etm.2021.10172. Epub 2021 May 10.ABSTRACTNon-steroidal anti-inflammatory drugs (NSAIDs) are widely used in the treatment of a variety of musculoskeletal conditions, injuries and after surgery for postoperative pain management. Their use has been associated with impaired bone healing, possibly due to a multifactorial function, which may include inhibition of osteoblast recruitment and differentiation. However, up to date, there is no consensus regarding the impact of NSAIDs on bone-healing. The aim of the current study was to investigate the effects of five NSAIDs on the cellul...
Source: Experimental and Therapeutic Medicine - May 28, 2021 Category: General Medicine Authors: Chrystalleni Hadjicharalambous Kalliopi Alpantaki Maria Chatzinikolaidou Source Type: research

Proteomics reveals urine apolipoprotein A-I as a potential biomarker of acute kidney injury following percutaneous coronary intervention in elderly patients
In conclusion, apoA-I may be a promising biomarker for the early diagnosis of PCI-AKI in elderly patients. The role of apoA-I in the pathobiology of PCI-AKI requires further exploration.PMID:34046095 | PMC:PMC8141962 | DOI:10.3892/etm.2021.10177 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - May 28, 2021 Category: General Medicine Authors: Fangfang Zhou Qun Luo Lina Han Gen Shen Lulu Huang Honghua Ye Source Type: research

Dynamics of serum α-fetoprotein in viral hepatitis C without hepatocellular carcinoma
In conclusion, the data revealed that a moderate increase in AFP levels could be present in patients with HCV even in the absence of HCC, unrelated to viral load or therapy response and that there was a linear positive correlation between serum levels of AFP and the degree of hepatic cytolysis and cholestasis. Additionally, mixed cryoglobulinemia was present in HCV patients with patent viral load, decreasing in those with SVR after therapy cessation unrelated to any renal impairment, while the degree of fibrosis was increased in HCV-infected patients, with no reversibility 12 weeks after successful therapy.PMID:34035846 | ...
Source: Experimental and Therapeutic Medicine - May 26, 2021 Category: General Medicine Authors: Teodora Isac Sebastian Isac Simona Ioanitescu Enyedi Mihaly Maria-Daniela Tanasescu Daniela Gabriela Balan Adrian Tulin Laura Iliescu Source Type: research

Management of large inflammatory dentigerous cysts adapted to the general condition of the patient: Two case reports
In conclusion, conservative treatment of large inflammatory dentigerous cysts in children gives good results with minimal intervention, ensures physiologic development of teeth and proper bone healing. The general condition of the patient can influence treatment choice. Patients must be followed up until eruption of the displaced permanent teeth and bony consolidation of the cyst.PMID:34035847 | PMC:PMC8135138 | DOI:10.3892/etm.2021.10182 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - May 26, 2021 Category: General Medicine Authors: Arina Vinereanu Andrada Bratu Andreea Didilescu Aneta Munteanu Source Type: research

Effects of withdrawing an atherogenic diet on the atherosclerotic plaque in rabbits
Exp Ther Med. 2021 Jul;22(1):751. doi: 10.3892/etm.2021.10183. Epub 2021 May 12.ABSTRACTLifestyle interventions and pharmacotherapy are the most common of non-invasive treatments for atherosclerosis, but the individual effect of diet on plaques remains unclear. The current study aimed to investigate the effect of withdrawing the atherogenic diet on plaque in the aortas of rabbits. Experimental atheroma was induced in 33 rabbits using a 1% high cholesterol diet for 30 days (H-30 d) or 90 days (H-90 d, baseline group). After 90 days of the atherogenic diet, the remaining animals were divided into four groups: A total of 10 r...
Source: Experimental and Therapeutic Medicine - May 26, 2021 Category: General Medicine Authors: Lijun Zhao Shifang Zhang Qiaoli Su Shuangqing Li Source Type: research

RPLP1 is highly expressed in hepatocellular carcinoma tissues and promotes proliferation, invasion and migration of human hepatocellular carcinoma Hep3b cells
Exp Ther Med. 2021 Jul;22(1):752. doi: 10.3892/etm.2021.10184. Epub 2021 May 12.ABSTRACTHepatocellular carcinoma (HCC) is a common primary malignant tumor with a high mortality rate. Increasing evidence suggests that ribosomal protein LP1 (RPLP1) is involved in the progression of different types of cancer. Thus, the present study aimed to investigate the underlying molecular mechanism of RPLP1 in HCC progression. The cellular behaviors of Hep3b cells were assessed via Cell Counting Kit-8, colony formation, wound healing and Transwell assays. Western blot analysis was performed to detect protein expression levels, while rev...
Source: Experimental and Therapeutic Medicine - May 26, 2021 Category: General Medicine Authors: Changji Xie Kai Cao Dexin Peng Lei Qin Source Type: research

Clinical application of whole-exome sequencing: A retrospective, single-center study
Exp Ther Med. 2021 Jul;22(1):753. doi: 10.3892/etm.2021.10185. Epub 2021 May 12.ABSTRACTThe aim of the present study was to assess the practical diagnostic value of whole-exome sequencing (WES) in patients with different phenotypes and to explore possible strategies to increase the capability of WES in identifying disease-causing genes. A total of 1,360 patients (aged from 1 day to 42 years old) with manifestations of genetic diseases were genotyped using WES and statistical analysis was performed on the results obtained. Within this cohort, the overall positive rate of identification of a disease-causing gene alteration w...
Source: Experimental and Therapeutic Medicine - May 26, 2021 Category: General Medicine Authors: Qiang Zhang Zailong Qin Shang Yi Hao Wei Xun Zhao Zhou Jiasun Su Source Type: research

Long non-coding RNA LUCAT1 promotes the progression of clear cell renal cell carcinoma via the microRNA-375/YAP1 axis
Exp Ther Med. 2021 Jul;22(1):754. doi: 10.3892/etm.2021.10186. Epub 2021 May 12.ABSTRACTClear cell renal cell carcinoma (ccRCC) is a common renal cell carcinoma with a high mortality rate. Lung cancer-associated transcript 1 (LUCAT1) has been reported to be a potential biomarker of prognosis in human ccRCC. However, the underlying mechanism of the function of LUCAT1 in ccRCC remains poorly understood. The present study aimed to investigate the role and underlying mechanism of LUCAT1 in ccRCC. The expression level of LUCAT1, microRNA-375 (miR-375) and yes-associated protein 1 (YAP1) in ccRCC tissues and cells was detected b...
Source: Experimental and Therapeutic Medicine - May 26, 2021 Category: General Medicine Authors: Xiaojun Wang Hui Ou Liangfen Zhou Hengyu Liu Xiaobao Liu Huiyun Zhang Source Type: research

Long non-coding RNA DUXAP8 promotes tumorigenesis by regulating IGF1R via miR-9-3p in hepatocellular carcinoma
Exp Ther Med. 2021 Jul;22(1):755. doi: 10.3892/etm.2021.10187. Epub 2021 May 12.ABSTRACTHepatocellular carcinoma (HCC) is the third leading cause of cancer-related death worldwide with a low 5-year survival rate. Long non-coding RNA (lncRNA) double homeobox A pseudogene 8 (DUXAP8) is an oncogene and a potential biomarker in various tumors, such as ovarian, colorectal and non-small-cell lung cancer. However, the function and molecular mechanism underlying DUXAP8 in HCC progression is not completely understood. The expression of DUXAP8, microRNA (miR)-9-3p and insulin-like growth factor 1 receptor (IGF1R) in HCC tissues and ...
Source: Experimental and Therapeutic Medicine - May 26, 2021 Category: General Medicine Authors: Qiang Guan Bo Yuan Xiaobin Zhang Tinghai Yan Jiangong Li Wuzhong Xu Source Type: research

COVID-19-related thyroid conditions (Review)
Exp Ther Med. 2021 Jul;22(1):756. doi: 10.3892/etm.2021.10188. Epub 2021 May 13.ABSTRACTIn patients who were not previously diagnosed with any thyroid conditions, the scenario of COVID-19-related anomalies of the hypothalamus-pituitary-thyroid axes may include either: A process of central thyroid stimulating hormone (TSH) disturbances via virus-related hypophysitis; an atypical type of subacute thyroiditis which is connected to the virus spread or to excessive cytokine production including a destructive process with irreversible damage of the gland or low T3 (triiodothyronine) syndrome (so called non-thyroid illness syndro...
Source: Experimental and Therapeutic Medicine - May 26, 2021 Category: General Medicine Authors: Florica Șandru Mara Carsote R ăzvan Cosmin Petca Ancuta Augustina Gheorghisan-Galateanu Aida Petca Ana Valea Mihai Cristian Dumitra șcu Source Type: research

Predictive factors of 30-day mortality in patients with traumatic subdural hematoma
Exp Ther Med. 2021 Jul;22(1):757. doi: 10.3892/etm.2021.10189. Epub 2021 May 13.ABSTRACTIn the present study, we aimed to assess and analyze the predictive factors of 30-day mortality in patients with acute subdural hematoma (ASDH) who underwent surgical intervention after traumatic brain injury (TBI). We conducted a retrospective study, which included a cohort of 135 consecutive patients diagnosed with ASDH who required surgical evacuation. We assessed the demographic and clinical data, the imaging data of the hematoma described by preoperative computed tomography (CT) and the type of neurosurgical intervention for hemato...
Source: Experimental and Therapeutic Medicine - May 26, 2021 Category: General Medicine Authors: Iulia-Sevastiana Pastor L ăcrimioara Perju Dumbravă Costel Siserman Hora țiu Stan Ioana Para Ioan Ștefan Florian Source Type: research

Clinical and therapeutic features of acute cholecystitis in diabetic patients
Exp Ther Med. 2021 Jul;22(1):758. doi: 10.3892/etm.2021.10190. Epub 2021 May 13.ABSTRACTThe present study aimed to compare the clinical, paraclinical, intraoperative findings, and postoperative complications in acute cholecystitis in diabetic patients vs. non-diabetic patients. A 2-year retrospective study was performed on the patients who underwent emergency cholecystectomy for acute cholecystitis between 2017 and 2019 at the 4th Department of Surgery, Emergency University Hospital Bucharest. The diabetic subgroup numbered 46 eligible patients and the non-diabetic one 287 patients. Demographics, the severity of the clinic...
Source: Experimental and Therapeutic Medicine - May 26, 2021 Category: General Medicine Authors: Dragos Serban Simona Andreea Balasescu Catalin Alius Cristian Balalau Alexandru Dan Sabau Cristinel Dumitru Badiu Bogdan Socea Andra Maria Trotea Ana Maria Dascalu Ion Motofei Valeriu Ardeleanu Radu Iulian Spataru Dan Sabau Gabriel Catalin Smarandache Source Type: research

Portal vein thrombosis: A concise review (Review)
Exp Ther Med. 2021 Jul;22(1):759. doi: 10.3892/etm.2021.10191. Epub 2021 May 13.ABSTRACTPortal vein thrombosis (PVT) is a frequent complication in cirrhotic patients, but it may also exist as a basic vascular condition even without any liver damage. Local and systemic factors play a significant role in the pathogenesis of PVT; yet, in practice, more than one factor may be identified. PVT can be considered a result of liver fibrosis and hepatic insufficiency. The JAK2 mutation has been accepted as a factor producing PVT. In general, the anticoagulants are recommended but this therapy should be used carefully in treating pat...
Source: Experimental and Therapeutic Medicine - May 26, 2021 Category: General Medicine Authors: Raluca S Costache Andreea S Dragomiric ă Elena A Dumitra ș Jinga Mariana Ana C ăruntu Andrada Popescu Daniel O Costache Source Type: research

Clinical, biological and electroencephalographic monitoring of newborns with neurological risk in the Neonatal Intensive Care Unit
In conclusion, common blood tests in association with aEEG monitoring and rigorous neurological assessment can predict short-term outcome of HIE and multiorgan dysfunction and can help clinicians predict even long-term outcomes in severe HIE.PMID:34035857 | PMC:PMC8135117 | DOI:10.3892/etm.2021.10192 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - May 26, 2021 Category: General Medicine Authors: Florina Marinela Doandes Aniko-Maria Manea Nicoleta Lungu Daniela Cioboata Timea Brandibur Oana Costescu Anca Hudisteanu Eugen Radu Boia Marioara Boia Source Type: research

circSKIL promotes the ossification of cervical posterior longitudinal ligament by activating the JNK/STAT3 pathway
Exp Ther Med. 2021 Jul;22(1):761. doi: 10.3892/etm.2021.10193. Epub 2021 May 13.ABSTRACTOssification of the posterior longitudinal ligament (OPLL) is a hyperostotic spinal condition that involves genetic factors as well as non-genetic factors, and its underlying molecular mechanism is largely unknown. Recently, circular RNAs (circRNAs) have been attracting the attention of researchers since they have important regulatory roles in many diseases, including bone metabolism disorders. The present study aimed to investigate the role of circRNA SKI-like proto-oncogene (circSKIL) in OPLL disease progression. First, primary poster...
Source: Experimental and Therapeutic Medicine - May 26, 2021 Category: General Medicine Authors: Naikun Sun Yunbang Liang Baoshan Hu Jinyi Feng Guangxun Lin Xin Chen Gang Rui Source Type: research

Role of bone morphogenic protein-4 in gestational diabetes mellitus-related hypertension
Exp Ther Med. 2021 Jul;22(1):762. doi: 10.3892/etm.2021.10194. Epub 2021 May 13.ABSTRACTHyperglycaemia stimulates the synthesis and release of bone morphogenetic protein-4 (BMP-4) in vascular endothelial cells, which further induces peroxide production and inflammatory responses, leading to vascular endothelial dysfunction. However, the role of BMP-4 in gestational diabetes mellitus (GDM)-related vascular endothelial dysfunction remains unclear. In the present study, the hypothesis that the overexpression of BMP-4 would induce GDM-related hypertension by impairing vascular endothelial function was evaluated. An animal mode...
Source: Experimental and Therapeutic Medicine - May 26, 2021 Category: General Medicine Authors: Benshuo Cai Juan Du Source Type: research

Septin 4 activates PPAR γ/LXRα signaling by upregulating ABCA1 and ABCG1 expression to inhibit the formation of THP-1 macrophage-derived foam cells
In conclusion, Septin 4 may serve an important role in preventing foam cell formation by activating PPARγ/LXRα signaling and subsequently enhancing ABCA1/G1 expression.PMID:34035860 | PMC:PMC8135116 | DOI:10.3892/etm.2021.10195 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - May 26, 2021 Category: General Medicine Authors: Xiaoying Song Guoliang Yan Haihui Wang Danfei Lou Source Type: research

Potential protective effect of hesperidin on hypoxia/reoxygenation-induced hepatocyte injury
Exp Ther Med. 2021 Jul;22(1):764. doi: 10.3892/etm.2021.10196. Epub 2021 May 13.ABSTRACTHesperidin (HDN) has been reported to have hydrogen radical- and hydrogen peroxide-removal activities and to serve an antioxidant role in biological systems. However, whether HDN protects hepatocytes (HCs) against hypoxia/reoxygenation (H/R)-induced injury remains unknown. The present study aimed to explore the role of HDN in H/R-induced injury. HCs were isolated and cultured under H/R conditions with or without HDN treatment. HC damage was markedly induced under H/R, as indicated by cell viability, supernatant lactate dehydrogenase lev...
Source: Experimental and Therapeutic Medicine - May 26, 2021 Category: General Medicine Authors: Shilai Li Jijin Zhu Ling Pan Peiqi Wan Quanlin Qin Daqing Luo Wenhui Pan Yuqing Wei Yansong Xu Liming Shang Xinping Ye Source Type: research

Dynamics of serum α-fetoprotein in viral hepatitis C without hepatocellular carcinoma
In conclusion, the data revealed that a moderate increase in AFP levels could be present in patients with HCV even in the absence of HCC, unrelated to viral load or therapy response and that there was a linear positive correlation between serum levels of AFP and the degree of hepatic cytolysis and cholestasis. Additionally, mixed cryoglobulinemia was present in HCV patients with patent viral load, decreasing in those with SVR after therapy cessation unrelated to any renal impairment, while the degree of fibrosis was increased in HCV-infected patients, with no reversibility 12 weeks after successful therapy.PMID:34035846 | ...
Source: Experimental and Therapeutic Medicine - May 26, 2021 Category: General Medicine Authors: Teodora Isac Sebastian Isac Simona Ioanitescu Enyedi Mihaly Maria-Daniela Tanasescu Daniela Gabriela Balan Adrian Tulin Laura Iliescu Source Type: research

Management of large inflammatory dentigerous cysts adapted to the general condition of the patient: Two case reports
In conclusion, conservative treatment of large inflammatory dentigerous cysts in children gives good results with minimal intervention, ensures physiologic development of teeth and proper bone healing. The general condition of the patient can influence treatment choice. Patients must be followed up until eruption of the displaced permanent teeth and bony consolidation of the cyst.PMID:34035847 | PMC:PMC8135138 | DOI:10.3892/etm.2021.10182 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - May 26, 2021 Category: General Medicine Authors: Arina Vinereanu Andrada Bratu Andreea Didilescu Aneta Munteanu Source Type: research

Effects of withdrawing an atherogenic diet on the atherosclerotic plaque in rabbits
Exp Ther Med. 2021 Jul;22(1):751. doi: 10.3892/etm.2021.10183. Epub 2021 May 12.ABSTRACTLifestyle interventions and pharmacotherapy are the most common of non-invasive treatments for atherosclerosis, but the individual effect of diet on plaques remains unclear. The current study aimed to investigate the effect of withdrawing the atherogenic diet on plaque in the aortas of rabbits. Experimental atheroma was induced in 33 rabbits using a 1% high cholesterol diet for 30 days (H-30 d) or 90 days (H-90 d, baseline group). After 90 days of the atherogenic diet, the remaining animals were divided into four groups: A total of 10 r...
Source: Experimental and Therapeutic Medicine - May 26, 2021 Category: General Medicine Authors: Lijun Zhao Shifang Zhang Qiaoli Su Shuangqing Li Source Type: research

RPLP1 is highly expressed in hepatocellular carcinoma tissues and promotes proliferation, invasion and migration of human hepatocellular carcinoma Hep3b cells
Exp Ther Med. 2021 Jul;22(1):752. doi: 10.3892/etm.2021.10184. Epub 2021 May 12.ABSTRACTHepatocellular carcinoma (HCC) is a common primary malignant tumor with a high mortality rate. Increasing evidence suggests that ribosomal protein LP1 (RPLP1) is involved in the progression of different types of cancer. Thus, the present study aimed to investigate the underlying molecular mechanism of RPLP1 in HCC progression. The cellular behaviors of Hep3b cells were assessed via Cell Counting Kit-8, colony formation, wound healing and Transwell assays. Western blot analysis was performed to detect protein expression levels, while rev...
Source: Experimental and Therapeutic Medicine - May 26, 2021 Category: General Medicine Authors: Changji Xie Kai Cao Dexin Peng Lei Qin Source Type: research

Clinical application of whole-exome sequencing: A retrospective, single-center study
Exp Ther Med. 2021 Jul;22(1):753. doi: 10.3892/etm.2021.10185. Epub 2021 May 12.ABSTRACTThe aim of the present study was to assess the practical diagnostic value of whole-exome sequencing (WES) in patients with different phenotypes and to explore possible strategies to increase the capability of WES in identifying disease-causing genes. A total of 1,360 patients (aged from 1 day to 42 years old) with manifestations of genetic diseases were genotyped using WES and statistical analysis was performed on the results obtained. Within this cohort, the overall positive rate of identification of a disease-causing gene alteration w...
Source: Experimental and Therapeutic Medicine - May 26, 2021 Category: General Medicine Authors: Qiang Zhang Zailong Qin Shang Yi Hao Wei Xun Zhao Zhou Jiasun Su Source Type: research

Long non-coding RNA LUCAT1 promotes the progression of clear cell renal cell carcinoma via the microRNA-375/YAP1 axis
Exp Ther Med. 2021 Jul;22(1):754. doi: 10.3892/etm.2021.10186. Epub 2021 May 12.ABSTRACTClear cell renal cell carcinoma (ccRCC) is a common renal cell carcinoma with a high mortality rate. Lung cancer-associated transcript 1 (LUCAT1) has been reported to be a potential biomarker of prognosis in human ccRCC. However, the underlying mechanism of the function of LUCAT1 in ccRCC remains poorly understood. The present study aimed to investigate the role and underlying mechanism of LUCAT1 in ccRCC. The expression level of LUCAT1, microRNA-375 (miR-375) and yes-associated protein 1 (YAP1) in ccRCC tissues and cells was detected b...
Source: Experimental and Therapeutic Medicine - May 26, 2021 Category: General Medicine Authors: Xiaojun Wang Hui Ou Liangfen Zhou Hengyu Liu Xiaobao Liu Huiyun Zhang Source Type: research

Long non-coding RNA DUXAP8 promotes tumorigenesis by regulating IGF1R via miR-9-3p in hepatocellular carcinoma
Exp Ther Med. 2021 Jul;22(1):755. doi: 10.3892/etm.2021.10187. Epub 2021 May 12.ABSTRACTHepatocellular carcinoma (HCC) is the third leading cause of cancer-related death worldwide with a low 5-year survival rate. Long non-coding RNA (lncRNA) double homeobox A pseudogene 8 (DUXAP8) is an oncogene and a potential biomarker in various tumors, such as ovarian, colorectal and non-small-cell lung cancer. However, the function and molecular mechanism underlying DUXAP8 in HCC progression is not completely understood. The expression of DUXAP8, microRNA (miR)-9-3p and insulin-like growth factor 1 receptor (IGF1R) in HCC tissues and ...
Source: Experimental and Therapeutic Medicine - May 26, 2021 Category: General Medicine Authors: Qiang Guan Bo Yuan Xiaobin Zhang Tinghai Yan Jiangong Li Wuzhong Xu Source Type: research

COVID-19-related thyroid conditions (Review)
Exp Ther Med. 2021 Jul;22(1):756. doi: 10.3892/etm.2021.10188. Epub 2021 May 13.ABSTRACTIn patients who were not previously diagnosed with any thyroid conditions, the scenario of COVID-19-related anomalies of the hypothalamus-pituitary-thyroid axes may include either: A process of central thyroid stimulating hormone (TSH) disturbances via virus-related hypophysitis; an atypical type of subacute thyroiditis which is connected to the virus spread or to excessive cytokine production including a destructive process with irreversible damage of the gland or low T3 (triiodothyronine) syndrome (so called non-thyroid illness syndro...
Source: Experimental and Therapeutic Medicine - May 26, 2021 Category: General Medicine Authors: Florica Șandru Mara Carsote R ăzvan Cosmin Petca Ancuta Augustina Gheorghisan-Galateanu Aida Petca Ana Valea Mihai Cristian Dumitra șcu Source Type: research

Predictive factors of 30-day mortality in patients with traumatic subdural hematoma
Exp Ther Med. 2021 Jul;22(1):757. doi: 10.3892/etm.2021.10189. Epub 2021 May 13.ABSTRACTIn the present study, we aimed to assess and analyze the predictive factors of 30-day mortality in patients with acute subdural hematoma (ASDH) who underwent surgical intervention after traumatic brain injury (TBI). We conducted a retrospective study, which included a cohort of 135 consecutive patients diagnosed with ASDH who required surgical evacuation. We assessed the demographic and clinical data, the imaging data of the hematoma described by preoperative computed tomography (CT) and the type of neurosurgical intervention for hemato...
Source: Experimental and Therapeutic Medicine - May 26, 2021 Category: General Medicine Authors: Iulia-Sevastiana Pastor L ăcrimioara Perju Dumbravă Costel Siserman Hora țiu Stan Ioana Para Ioan Ștefan Florian Source Type: research

Clinical and therapeutic features of acute cholecystitis in diabetic patients
Exp Ther Med. 2021 Jul;22(1):758. doi: 10.3892/etm.2021.10190. Epub 2021 May 13.ABSTRACTThe present study aimed to compare the clinical, paraclinical, intraoperative findings, and postoperative complications in acute cholecystitis in diabetic patients vs. non-diabetic patients. A 2-year retrospective study was performed on the patients who underwent emergency cholecystectomy for acute cholecystitis between 2017 and 2019 at the 4th Department of Surgery, Emergency University Hospital Bucharest. The diabetic subgroup numbered 46 eligible patients and the non-diabetic one 287 patients. Demographics, the severity of the clinic...
Source: Experimental and Therapeutic Medicine - May 26, 2021 Category: General Medicine Authors: Dragos Serban Simona Andreea Balasescu Catalin Alius Cristian Balalau Alexandru Dan Sabau Cristinel Dumitru Badiu Bogdan Socea Andra Maria Trotea Ana Maria Dascalu Ion Motofei Valeriu Ardeleanu Radu Iulian Spataru Dan Sabau Gabriel Catalin Smarandache Source Type: research

Portal vein thrombosis: A concise review (Review)
Exp Ther Med. 2021 Jul;22(1):759. doi: 10.3892/etm.2021.10191. Epub 2021 May 13.ABSTRACTPortal vein thrombosis (PVT) is a frequent complication in cirrhotic patients, but it may also exist as a basic vascular condition even without any liver damage. Local and systemic factors play a significant role in the pathogenesis of PVT; yet, in practice, more than one factor may be identified. PVT can be considered a result of liver fibrosis and hepatic insufficiency. The JAK2 mutation has been accepted as a factor producing PVT. In general, the anticoagulants are recommended but this therapy should be used carefully in treating pat...
Source: Experimental and Therapeutic Medicine - May 26, 2021 Category: General Medicine Authors: Raluca S Costache Andreea S Dragomiric ă Elena A Dumitra ș Jinga Mariana Ana C ăruntu Andrada Popescu Daniel O Costache Source Type: research

Clinical, biological and electroencephalographic monitoring of newborns with neurological risk in the Neonatal Intensive Care Unit
In conclusion, common blood tests in association with aEEG monitoring and rigorous neurological assessment can predict short-term outcome of HIE and multiorgan dysfunction and can help clinicians predict even long-term outcomes in severe HIE.PMID:34035857 | PMC:PMC8135117 | DOI:10.3892/etm.2021.10192 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - May 26, 2021 Category: General Medicine Authors: Florina Marinela Doandes Aniko-Maria Manea Nicoleta Lungu Daniela Cioboata Timea Brandibur Oana Costescu Anca Hudisteanu Eugen Radu Boia Marioara Boia Source Type: research