Thymosin β10 mediates the effects of microRNA-184 in the proliferation and epithelial-mesenchymal transition of BCPAP cells
Exp Ther Med. 2021 Jul;22(1):742. doi: 10.3892/etm.2021.10174. Epub 2021 May 11.ABSTRACTThyroid cancer is the most common malignant tumor of the endocrine system. It has been reported that thymosin β10 (TMSB10) serves a vital role in tumor invasion and metastasis, and further understanding the role of TMSB10 in thyroid cancer may provide new insights into the development of novel targeted drugs. Bioinformatics analysis suggested that there might exist a regulatory relationship between miR-184 and TMSB10. Therefore, the expression of microRNA (miR)-184 was investigated in the TPC-1 and BCPAP thyroid cancer cell lines a...
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Cheng Yang Yunni Liu Kun Fang Source Type: research

Rosiglitazone alleviates lipopolysaccharide-induced inflammation in RAW264.7 cells via inhibition of NF- κB and in a PPARγ-dependent manner
Exp Ther Med. 2021 Jul;22(1):743. doi: 10.3892/etm.2021.10175. Epub 2021 May 11.ABSTRACTRosiglitazone is a synthetic peroxisome proliferator-activated receptor (PPAR)γ agonist widely used for the treatment of type 2 diabetes. Recent studies have demonstrated that rosiglitazone displays anti-inflammatory effects. The present study aimed to investigate whether rosiglitazone alleviates decreases in RAW264.7 cell viability resulting from lipopolysaccharide (LPS)-induced inflammation, as well as exploring the underlying mechanism. A macrophage inflammatory injury model was established by treating RAW264.7 cells with 100 n...
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Jing-Ping Zhou Xiao-Ning Yang Yang Song Fei Zhou Jing-Jing Liu Yi-Qun Hu Li-Gang Chen Source Type: research

miR-489-3p inhibits TLR4/NF- κB signaling to prevent inflammation in psoriasis
Exp Ther Med. 2021 Jul;22(1):744. doi: 10.3892/etm.2021.10176. Epub 2021 May 11.ABSTRACTPsoriasis is a chronic inflammatory skin disease whose etiology has not yet been determined. MicroRNAs (miRs) regulate the early stages of psoriasis and are targets for therapeutic intervention. The present study aimed to investigate the functional role of miR-489-3p in psoriasis. The present study first assessed the expression levels of miR-489-3p and Toll-like receptor (TLR)4 mRNA using reverse transcription-quantitative PCR, and also detected the protein expression levels of TLR4 and NF-κB via western blot analysis. TargetScan ...
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Yujian Ye Ping Wang Fangmei Zhou Source Type: research

Nephrogenic diabetes insipidus in children (Review)
Exp Ther Med. 2021 Jul;22(1):746. doi: 10.3892/etm.2021.10178. Epub 2021 May 11.ABSTRACTNephrogenic diabetes insipidus (NDI) is characterized by impaired urinary concentrating ability, despite normal or elevated plasma concentrations of the antidiuretic hormone, arginine vasopressin (AVP). NDI can be inherited or acquired. NDI can result from genetic abnormalities, such as mutations in the vasopressin V2 receptor (AVPR2) or the aquaporin-2 (AQP2) water channel, or acquired causes, such as chronic lithium therapy. Congenital NDI is a rare condition. Mutations in AVPR2 are responsible for approximately 90% of patients with c...
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Carmen Duicu Ana Maria Pitea Oana Maria S ăsăran Iulia Cozea Lidia Man Claudia B ănescu Source Type: research

Comparative hematological profiles for dose-dense vs. regular anthracycline-based neoadjuvant chemotherapy in non-metastatic breast cancer
Exp Ther Med. 2021 Jul;22(1):747. doi: 10.3892/etm.2021.10179. Epub 2021 May 11.ABSTRACTThe aim of the present study was to examine both the feasibility and toxicity of neoadjuvant dose-dense chemotherapy in women with non-metastatic breast cancer. A search within the OncoHelp Association breast cancer database has been performed in order to identify all non-metastatic breast cancer patients who underwent an initial consultation with a medical oncologist between March 2016 and April 2020. The inclusion criteria used were: i) Age, ii) follow-up care obtained at OncoHelp Association, iii) the intent to treat with a neoadjuva...
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Dorel Popovici Cristina Oprean Sorin S ăftescu Alina Negru Mihnea Munteanu Horia T Stanca Adrian Teodoru Simona Stanca Șerban Negru Source Type: research

Antibiotic resistance patterns of urinary tract pathogens in children from Central Romania
Exp Ther Med. 2021 Jul;22(1):748. doi: 10.3892/etm.2021.10180. Epub 2021 May 12.ABSTRACTOne of the most frequent bacterial infections in children are urinary tract infections (UTIs). In recent years, an increasing incidence of UTIs caused by resistant bacterial strains has been observed, especially with extended-spectrum β-lactamase-producing Enterobacteriaceae that represent about 15% of UTIs. A retrospective study was performed comprising 331 pediatric cases with UTI. Our study aimed to detect the resistance of the uropathogens to common drugs used in UTI treatment. High resistance rates have been recorded for ampic...
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Carmen Duicu Iulia Cozea Dan Delean Andreea Alexandra Aldea Cornel Aldea Source Type: research

Magnetic resonance imaging in schizophrenia: Luxury or necessity? (Review)
Exp Ther Med. 2021 Jul;22(1):765. doi: 10.3892/etm.2021.10197. Epub 2021 May 14.ABSTRACTSchizophrenia, one of the most common psychiatric disorders, with a worldwide annual incidence rate of approximately 0.3-0.7%, known to affect the population below 25 years of age, is persistent throughout lifetime and includes people from all layers of society. With recent technological progress that allows better imaging techniques, such as the ones provided by computed tomography and particularly magnetic resonance imaging (MRI), research on schizophrenia imaging has grown considerably. The purpose of this review is to establish the ...
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Floris Petru Iliuta Mihnea Costin Manea Magdalena Budisteanu Adela Magdalena Ciobanu Mirela Manea Source Type: research

< em > Helicobacter pylori < /em > infection over bile reflux: No influence on the severity of endoscopic or premalignant gastric lesion development
This study investigated the histologic gastric changes in patients with primary DGR and H. pylori infection, as well as their endoscopic findings, symptoms, drug consumption, and social behavior in comparison with patients presenting only DGR. The study included 560 patients with primary DGR on endoscopy divided into two groups, according to the presence/absence of H. pylori infection on biopsy (utilizing usual stainings and immunohistochemical methods). There was no significant difference in terms of age and sex, nor in the frequency of diabetes or esophagitis between the studied groups. Epigastric pain was associated wit...
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Andreea Sz őke Simona Mocan Anca Negovan Source Type: research

Association of oral Epstein-Barr virus with periodontal health in Japanese adults
Exp Ther Med. 2021 Jul;22(1):767. doi: 10.3892/etm.2021.10199. Epub 2021 May 17.ABSTRACTPrevious studies have demonstrated that oral Epstein-Barr virus (EBV) is associated with periodontitis. However, the relationship between periodontitis and oral EBV has not been fully elucidated by reducing the effects of confounding factors. The aim of the present study was to clarify the association between oral Epstein-Barr virus (EBV) and oral health status among middle-aged and older Japanese individuals. A total of 124 patients (46 males and 78 females; mean age, 69.2 years; age range, 35-90 years) who visited Hiroshima University...
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Cheng-Yih Su Hideo Shigeishi Hiroshi Murodumi Masaru SugiyaMa Kouji Ohta Toshinobu Takemoto Source Type: research

Silencing miRNA-1297 suppresses the invasion and migration of prostate cancer cells via targeting modulation of PTEN and blocking of the AKT/ERK pathway
Exp Ther Med. 2021 Jul;22(1):768. doi: 10.3892/etm.2021.10200. Epub 2021 May 17.ABSTRACTPhosphatase and tensin homolog (PTEN) loss is a major contributing factor of prostate cancer (PC). miRNA-1297 was reported to serve role in various cancer types; however, the potential roles of miRNA-1297 in PC had not been investigated. In the present study, tumor and adjacent tissues were collected from patients with PC. The gene expression level of miRNA-1297 was measured via polymerase chain reaction. Results indicated that the miRNA-1297 was overexpressed in tumor tissues from PC patients and in PC cell lines. miRNA-1297 also contr...
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Lei Wang Jing Gao Yu Zhang Shaosan Kang Source Type: research

Cascades between miRNAs, lncRNAs and the NF- κB signaling pathway in gastric cancer (Review)
Exp Ther Med. 2021 Jul;22(1):769. doi: 10.3892/etm.2021.10201. Epub 2021 May 17.ABSTRACTGastric cancer is a common digestive tract malignancy that is mainly treated with surgery combined with perioperative adjuvant chemoradiotherapy and biological targeted therapy. However, the diagnosis rate of early gastric cancer is low and both postoperative recurrence and distant metastasis are thorny problems. Therefore, it is essential to study the pathogenesis of gastric cancer and search for more effective means of treatment. The nuclear factor-κB (NF-κB) signaling pathway has an important role in the occurrence and de...
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Risheng Chen Mingxiu Yang Weiguo Huang Baiyun Wang Source Type: research

Silencing of kallikrein-related peptidase 6 attenuates the proliferation, migration, and invasion of gastric cancer cells through inhibition of epithelial-mesenchymal transition
In conclusion, KLK6 inhibition suppressed the proliferation, migration and invasion of gastric cancer cells both in vitro and in vivo through the inhibition of EMT. These findings indicate that KLK6 a potential therapeutic target for gastric cancer therapy.PMID:34055069 | PMC:PMC8145264 | DOI:10.3892/etm.2021.10202 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Dong Zhou Yanping He Hengping Li Weidong Huang Source Type: research

Oxidative stress and peripartum outcomes (Review)
Exp Ther Med. 2021 Jul;22(1):771. doi: 10.3892/etm.2021.10203. Epub 2021 May 17.ABSTRACTPregnancy, labor and childbirth are accompanied by excessive oxidative aggression. The excessive formation of free radicals [reactive oxygen species (ROS), reactive nitrogen species (RNS), chlorine reactive species (CRS)] causes cellular oxidative damage, which can be scavenged by enzymatic or non-enzymatic antioxidants in normal healthy pregnancy, physiological labor and delivery without any complications. An imbalance between the pro-oxidant and antioxidant factors may lead to oxidative stress, which contributes to the development of ...
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Zsuzsanna Simon-Szabo Erzsebet Fogarasi Eniko Nemes-Nagy Lorand Denes Mircea Croitoru Bela Szabo Source Type: research

The importance of adrenal venous sampling in ACTH-independent Cushing syndrome: A case report and literature review
Exp Ther Med. 2021 Jul;22(1):772. doi: 10.3892/etm.2021.10204. Epub 2021 May 18.ABSTRACTIndependent Cushing's adrenocorticotropic hormone (ACTH) syndrome can have several causes, including adrenal carcinoma or simple adrenal hyperplasia. Although the distinction between malignant and benign can be effectively made through imaging investigations, in the situation where there are bilateral formations, their hormonal activity is impossible to appreciate via non-invasive techniques. The present study includes the presentation of a clinical case on the basis of which a literature review was made. The clinical case pertains to a...
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Raluca Tulin Adrian Tulin Luminita Florentina Tomescu Enyedi Mihaly Adelaida Avino Bogdan Socea Cristian-Radu Jecan Ovidiu Stiru Radu-Iulian Spataru Source Type: research

Tumoral markers in bladder cancer (Review)
Exp Ther Med. 2021 Jul;22(1):773. doi: 10.3892/etm.2021.10205. Epub 2021 May 18.ABSTRACTBladder tumors are frequently diagnosed urologic malignant diseases with an extremely high recurrence rate compared to other neoplastic tumors. Urothelial bladder carcinomas are mostly identified in their incipient form, as non-muscle invasive, but despite that, a third of them develop into aggressive recurrent disease. The diagnosis of bladder carcinoma at this moment is established using cytology and cystoscopy and is a great challenge for clinicians due to the lack of sensitivity. Urinary biomarkers could improve and enhance the diag...
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Ovidiu Bratu Dragos Marcu Radu Anghel Dan Spinu Lucian Iorga Irina Balescu Nicolae Bacalbasa Camelia Diaconu Cornel Savu Carmen Savu Alexandru Cherciu Source Type: research

B7 homolog 6 promotes the progression of cervical cancer
In conclusion, the findings of the present study suggested that B7-H6 may serve as a novel oncogene and may hold promise as a potential therapeutic target for cervical cancer.PMID:34055073 | PMC:PMC8145428 | DOI:10.3892/etm.2021.10206 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Ruimeng Guo Guoyan Liu Changying Li Xuejing Liu Yanying Xu Weina Yang Fang Wang Source Type: research

Diabetic neuropathic pain induced by streptozotocin alters the expression profile of non-coding RNAs in the spinal cord of mice as determined by sequencing analysis
Exp Ther Med. 2021 Jul;22(1):775. doi: 10.3892/etm.2021.10207. Epub 2021 May 18.ABSTRACTDiabetic neuropathic pain (DNP) is one of the most serious complications of diabetes. Patients with DNP always exhibit spontaneous and stimulus-evoked pain. However, the pathogenesis of DNP remains to be fully elucidated. Non-coding RNAs (ncRNAs) serve important roles in several cellular processes and dysregulated expression may result in the development of several diseases, including DNP. Although ncRNAs have been suggested to be involved in the pathogenesis of DNP, their precise roles remain to be determined. In the present study, seq...
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Jian He Han Bin Wang Jiang Ju Huang Lei Zhang Dong Lin Li Wan You He Qing Ming Xiong Zai Sheng Qin Source Type: research

CLCA2 suppresses the proliferation, migration and invasion of cervical cancer
In conclusion, the results of the present study demonstrated that CLCA2 suppressed the proliferation, migration and invasion of cervical cancer cells, and that CLCA2 may be a potential therapeutic target of cervical cancer.PMID:34055075 | PMC:PMC8145432 | DOI:10.3892/etm.2021.10208 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Peijin Zhang Yang Lin Yaqiong Liu Source Type: research

Analysis of the effects of prepared porcelain veneers and unprepared porcelain veneers on gingival crevicular flora based on high-throughput sequencing
Exp Ther Med. 2021 Jul;22(1):777. doi: 10.3892/etm.2021.10209. Epub 2021 May 18.ABSTRACTThe effect of tooth preparation on the gingival crevicular flora of abutment teeth during porcelain veneer treatment is not clear. The purpose of the present study was to analyze the difference between prepared porcelain veneers and unprepared porcelain veneers on gingival crevicular flora. High-throughput sequencing was used in the present study. A total of 20 patients (40 anterior teeth) with veneer restoration of anterior teeth were enrolled. They were divided into two groups: The prepared porcelain veneer group (group P, 11 cases, 1...
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Rui Zhang Lanhua Shen Dandan Xu Xiao Li Source Type: research

rs1344706 polymorphism of zinc finger protein 804a (ZNF804a) gene related to the integrity of white matter fiber bundle in schizophrenics
Exp Ther Med. 2021 Jul;22(1):778. doi: 10.3892/etm.2021.10210. Epub 2021 May 18.ABSTRACTGenetic factors play an important role in the pathogenesis of schizophrenia (SZ), and the zinc finger protein 804a (ZNF804a) gene has been considered to be a risk gene for schizophrenia. In the present study, the correlation between rs1344706 polymorphism of ZNF804a gene and the integrity of white matter in schizophrenic cases was explored. A total of 60 SZ patients and 100 healthy controls (HC) were included to undergo head MRI. According to the genotyping of rs1344706 in ZNF804a, the subjects in each group were divided into a normal a...
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Jian Zhou Quan Bao Shuang Liang Hong Guo Xin Meng Guangfeng Zhang Ping Li Source Type: research

Expression and prognostic value of SULT1A2 in bladder cancer
Exp Ther Med. 2021 Jul;22(1):779. doi: 10.3892/etm.2021.10211. Epub 2021 May 19.ABSTRACTSulfotransferase Family 1A Member 2 (SULT1A2) is a protein coding gene. Several studies have reported that SULT1A2 may have a chemical carcinogenic effect if expressed as a functional protein. The present study aimed to investigate the expression and potential role of SULT1A2 in bladder cancer (BC). Data from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus databases were used to analyze SULT1A2 expression in BC. In addition, reverse transcription-quantitative PCR and western blot analyses were performed to detect SULT1A2 expr...
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Yinghui Chao Qifeng Ou Jin Shang Source Type: research

miR-3677-5p promotes the proliferation, migration and invasion of hepatocellular carcinoma cells and is associated with prognosis
In conclusion, the present study demonstrated that miR-3677-5p acts as an oncogene that has a critical role in the regulation of HCC proliferation and progression. Hence, miR-3677-5p may serve as a valuable prognostic biomarker and may be developed as a promising therapeutic target for HCC.PMID:34055079 | PMC:PMC8145920 | DOI:10.3892/etm.2021.10212 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Hai-Xiang Mao Bai-Wen Chen Jie Wang Chen-Yang Ma Yi-Chao Gan Kai-Jie Qiu Source Type: research

Long non-coding RNA Rian protects against experimental bronchopulmonary dysplasia by sponging miR-421
Exp Ther Med. 2021 Jul;22(1):781. doi: 10.3892/etm.2021.10213. Epub 2021 May 19.ABSTRACTBronchopulmonary dysplasia (BPD) is a frequent complication characterized by accelerated lung alveolarization in newborns. Long non-coding RNAs (lncRNAs) and microRNAs (miRs) are regarded as essential regulators in various diseases, including BPD. However, the detailed mechanism of the functions of RNA imprinted and accumulated in nucleus (Rian) lncRNA in the progression of BPD have remained elusive. The aim of the present study was to illustrate the interaction between miR-421 and Rian in BPD models and MLE-12 cells. The ability of Ria...
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Xifeng Tao Yafei Fang Chen Huo Source Type: research

Ginsenoside Rg1 alleviates lipopolysaccharide-induced neuronal damage by inhibiting NLRP1 inflammasomes in HT22 cells
Exp Ther Med. 2021 Jul;22(1):782. doi: 10.3892/etm.2021.10214. Epub 2021 May 19.ABSTRACTLipopolysaccharide (LPS) is a toxic component of cell walls of Gram-negative bacteria that are widely present in gastrointestinal tracts. Increasing evidence showed that LPS plays important roles in the pathogeneses of neurodegenerative disorders, such as Alzheimer's disease (AD). NADPH oxidase s2 (NOX2) is a complex membrane protein that contributes to the production of reactive oxygen species (ROS) in several neurological diseases. The NLRP1 inflammasome can be activated in response to an accumulation of ROS in neurons. However, it is...
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Yaodong Zhang Shixin Ding Yali Chen Zhenghao Sun Junyan Zhang Yuli Han Xianan Dong Zhirui Fang Weizu Li Source Type: research

Curcumin ameliorates ischemic stroke injury in rats by protecting the integrity of the blood-brain barrier
Exp Ther Med. 2021 Jul;22(1):783. doi: 10.3892/etm.2021.10215. Epub 2021 May 19.ABSTRACTThe blood-brain barrier (BBB) is critical for proper cerebral homeostasis and its dysfunction during ischemic stroke can result in significant neurological injury. The major goal of the present study was to identify whether curcumin pretreatment possessed protective effects on BBB integrity during the 24 h of acute ischemic brain injury. To investigate the protective effects of curcumin, male Sprague-Dawley rats were divided into multiple groups, including sham, middle cerebral artery occlusion/reperfusion (MCAO/R) vehicle and curcumin ...
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Shuguang Wu Ting Guo Wenxuan Qi Yuyu Li Jie Gu Cui Liu Yuehong Sha Baocheng Yang Shuqun Hu Xuemei Zong Source Type: research

Bioinformatics identification of hub genes and signaling pathways regulated by intravenous immunoglobulin treatment in acute Kawasaki disease
Exp Ther Med. 2021 Jul;22(1):784. doi: 10.3892/etm.2021.10216. Epub 2021 May 19.ABSTRACTKawasaki disease (KD) is an acute, self-limiting form of vasculitis commonly encountered in infants and young children. Intravenous immunoglobulin (IVIG) is the primary drug used for the treatment of KD, which may significantly reduce the occurrence of coronary artery lesions. However, the specific molecular profile changes of KD caused by IVIG treatment have remained elusive and require further research. The present study was designed to identify key genes, pathways and immune cells affected by IVIG treatment using multiple bioinformat...
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Hongbiao Huang Lei Xu Yueyue Ding Jie Qin Chengcheng Huang Xuan Li Yunjia Tang Guanghui Qian Haitao Lv Source Type: research

Etomidate attenuates hyperoxia-induced acute lung injury in mice by modulating the Nrf2/HO-1 signaling pathway
Exp Ther Med. 2021 Jul;22(1):785. doi: 10.3892/etm.2021.10217. Epub 2021 May 19.ABSTRACTThe present study aimed to investigate the protective effects of etomidate on hyperoxia-induced acute lung injury in mice, particularly on the nuclear factor-erythroid 2-related factor 2 (Nrf2)/heme oxygenase 1 (HO-1) pathway. Fifty specific pathogen-free mice were randomly divided into the blank control, model, high oxygen exposure + low etomidate dose (0.3 mg·kg-1), a high oxygen exposure + moderate etomidate dose (3 mg·kg-1), and a high oxygen exposure + high etomidate dose (10 mg·kg-1) groups, with ten mice allo...
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Liming Jia Hongzhong Hao Chunyu Wang Jianfeng Wei Source Type: research

Comparison of modified corneal cross-linking with intrastromal voriconazole for the treatment of fungal corneal ulcer
In conclusion, the present study suggested that CXL could have a beneficial impact for treating fungal corneal ulcers in the aspects of infection control, localized lesions and accelerated epithelialization. In addition, except the loss of corneal transparency, this treatment approach could be applied with reduced risks of adverse events.PMID:34055085 | PMC:PMC8145909 | DOI:10.3892/etm.2021.10218 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Yingxin Chen Xingya Miao Minghong Gao Lixin Song Source Type: research

Modulated electro-hyperthermia with weekly paclitaxel or cisplatin in patients with recurrent or persistent epithelial ovarian, fallopian tube or primary peritoneal carcinoma: The KGOG 3030 trial
In conclusion, mEHT therapy with weekly paclitaxel or cisplatin appeared safe and warrants further investigation. The present trial was registered with www.clinicaltrials.gov on January 22, 2015 (trial registration no. NCT02344095).PMID:34055086 | PMC:PMC8145814 | DOI:10.3892/etm.2021.10219 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Kidong Kim Jae-Hoon Kim Seung Cheol Kim Yong Beom Kim Byung-Ho Nam Jae Hong No Hanbyoul Cho Woong Ju Dong Hoon Suh Yun Hwan Kim Source Type: research

Oncogenic role of epithelial cell transforming sequence 2 in lung adenocarcinoma cells
Exp Ther Med. 2021 Jul;22(1):788. doi: 10.3892/etm.2021.10220. Epub 2021 May 21.ABSTRACT[This retracts the article DOI: 10.3892/etm.2016.3584.].PMID:34055087 | PMC:PMC8145908 | DOI:10.3892/etm.2021.10220 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Hongyi Tan Xiaoshan Wang Xiaogang Yang Haitao Li Ben Liu Pinhua Pan Source Type: research

Combination of endothelial progenitor cells and BB-94 significantly alleviates brain damage in a mouse model of diabetic ischemic stroke
Exp Ther Med. 2021 Jul;22(1):789. doi: 10.3892/etm.2021.10221. Epub 2021 May 21.ABSTRACTIschemic stroke is a complication of chronic macrovascular disease in type 2 diabetes. However, the pathogenesis of diabetic ischemic stroke has not yet been fully clarified. The aim of the present study was to investigate the underlying effects of endothelial progenitor cells (EPCs) and the matrix metalloproteinase inhibitor BB-94 on diabetic stroke. In vitro experiments were performed using oxygen-glucose deprivation/reoxygenation (OGD/R) model cells, established using HT22 mouse hippocampal cells. MTT assays and flow cytometry reveal...
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Daixuan Zhou Zhi Huang Xiaoxi Zhu Tao Hong Yuanli Zhao Source Type: research

Anti-inflammatory role of curcumin in retinal disorders (Review)
Exp Ther Med. 2021 Jul;22(1):790. doi: 10.3892/etm.2021.10222. Epub 2021 May 21.ABSTRACTCurcumin [1,7-bis-(4-hydroxy-3-methoxyphenyl)-hepta-1,6-diene-3,5-dione], the main component of turmeric (Curcuma longa, a flowering plant of the ginger family, Zingiberaceae), is known to possess different pharmacological activities, particularly anti-inflammatory and antioxidant properties. Since an underlying inflammatory process exists in several ocular conditions, such as anterior uveitis, glaucoma, age-related macular degeneration (AMD) and diabetic retinopathy (DR), the aim of the present review was to summarize the pleiotropic e...
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Federica Franzone Marcella Nebbioso Tiziano Pergolizzi Giuseppe Attanasio Angela Musacchio Antonio Greco Paolo Giuseppe Limoli Marco Artico Demetrios A Spandidos Samanta Taurone Enzo Agostinelli Source Type: research

Long non-coding RNA HIT000218960 is associated with poor prognosis in patients with gastric cancer
In conclusion, HIT000218960 was highly expressed in patients with GC and was related to poor prognosis.PMID:34055048 | PMC:PMC8145346 | DOI:10.3892/etm.2021.10126 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Kunbo Dong Li Bai Deyong Tong Xiuna Shi Sirong Wei Yongguo Cai Source Type: research

Risk factors for positive sentinel lymph node, lymphatic or hematogenous dissemination over time in patients with cutaneous melanoma
In conclusion, the predictors that influence the occurrence of lymphatic or hematogenous metastases may differ, ulceration and positive sentinel lymph node being common for both types of metastatic spread, while Breslow index being a significant predictor only for hematogenous metastases. Male sex and the presence of ulceration were demonstrated to be significant risk factors for positive sentinel lymph nodes.PMID:34055049 | PMC:PMC8145265 | DOI:10.3892/etm.2021.10162 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Alexandra S Berghe Gheorghe Cobzac George Dindelegan Simona C Șenilă Corina I Baican Carolina M Solomon Liliana Rogojan Daniel C Leucu ța Tudor C Drugan Sorana D Bolboac ă Source Type: research

Effects of propofol on the proliferation and migration of liver cancer cells
In conclusion, the findings of the present study revealed that propofol exerted no effect on the viability, proliferation and migration of HepG2 and HCCLM3 cells under normoxic and hypoxic conditions.PMID:34055050 | PMC:PMC8138278 | DOI:10.3892/etm.2021.10165 (Source: Experimental and Therapeutic Medicine)
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Chan Li Qingxia Fu Jin Cai Hongxia Mei Wangning Shangguan Source Type: research

lncRNA WT1-AS is upregulated in osteoporosis and regulates the apoptosis of osteoblasts by interacting with p53
Exp Ther Med. 2021 Jul;22(1):734. doi: 10.3892/etm.2021.10166. Epub 2021 May 9.ABSTRACTIn cervical cancer, cellular tumor antigen p53 (p53) interacts with long non-coding WT1 antisense RNA (WT1-AS) and this protein serves an important role in osteoporosis. The present study aimed to investigate the role of WT1-AS in osteoporosis. WT1-AS was upregulated in the plasma of patients with osteoporosis and was positively correlated with p53 expression. Altered expression of WT1-AS and p53 separated patients with osteoporosis from healthy controls. Expression levels of WT1-AS and p53 decreased with prolonged treatment. In osteobla...
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Chaoqun Wang Quan Xie Wen Sun Ying Zhou Yu Liu Source Type: research

Decrease of ABCB1 protein expression and increase of G < sub > 1 < /sub > phase arrest induced by oleanolic acid in human multidrug-resistant cancer cells
Exp Ther Med. 2021 Jul;22(1):735. doi: 10.3892/etm.2021.10167. Epub 2021 May 9.ABSTRACTOleanolic acid (OA) is a natural compound that can be found in a number of edible and medicinal plants and confers diverse biological actions. However, the direct target of OA in human tumor cells remains poorly understood, preventing its application in clinical and health settings. A previous study revealed that overexpression of caveolin-1 in human leukemia HL-60 cells can increase its sensitivity to OA. The present study aimed to investigate the effects of OA on the doxorubicin-resistant human breast cancer MCF-7 cell line (MCF-7/DOX)...
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Didi Wang Jincai Wang Juan Zhang Xin Yi Jinhua Piao Li Li Jianjie Wang Pengxia Zhang Qiyang He Source Type: research

Bcl-xL mutant promotes cartilage differentiation of BMSCs by upregulating TGF- β/BMP expression levels
Exp Ther Med. 2021 Jul;22(1):736. doi: 10.3892/etm.2021.10168. Epub 2021 May 9.ABSTRACTBcl-xL is a transmembrane molecule in the mitochondria, with apoptosis-related and pro-metabolic functions, that also plays a role in chondrogenesis and differentiation. A Bcl-xL mutant, in which the GRI sequence is replaced by ELN, has no anti-apoptotic effect, while other biological functions of this mutant remain unchanged. The present study investigated the impact of this Bcl-xL mutant on cartilage differentiation and the expression levels of TGF-β and bone morphogenetic protein (BMP). Human bone marrow mesenchymal stem cells (B...
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Kai Xiao Lin Yang Wei Xie Xinfeng Gao Ruokun Huang Ming Xie Source Type: research

Inhibition of the Wnt/ β-catenin signaling pathway reduces autophagy levels in complement treated podocytes
Exp Ther Med. 2021 Jul;22(1):737. doi: 10.3892/etm.2021.10169. Epub 2021 May 9.ABSTRACTIn idiopathic membranous nephropathy, the complement membrane attack complex, more commonly referred to as complement 5b-9 (C5b-9), induces glomerular epithelial cell injury and proteinuria. C5b-9 can also activate numerous mechanisms that restrict or facilitate injury. Recent studies suggest that autophagy and the canonical Wnt signaling pathway serve an important role in repairing podocyte injury. However, the effect of C5b-9 on these pathways and the relationship between them remains unclear. The aim of the present study was to show t...
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Zhaocheng Dong Haoran Dai Yu Gao Zhendong Feng Wenbin Liu Fei Liu Zihan Zhang Fang Ma Xinran Xie Zebing Zhu Weijing Liu Baoli Liu Source Type: research

Expression of STAT3 and vasculogenic mimicry in gallbladder carcinoma promotes invasion and metastasis
Exp Ther Med. 2021 Jul;22(1):738. doi: 10.3892/etm.2021.10170. Epub 2021 May 9.ABSTRACTSurgical treatment of gallbladder carcinoma remains challenging, while targeted therapy has been demonstrated to have potential. In the present study, the effect of signal transducer and activator of transcription 3 (STAT3) expression and vasculogenic mimicry (VM) on the occurrence and development of gallbladder carcinoma was evaluated. A total of 72 patients with gallbladder carcinoma and 10 patients with chronic cholecystitis were examined. Immunohistochemical staining was performed to determine the positive expression rates of STAT3. ...
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Hongbing Zhou Yin Yuan Haixin Qian Source Type: research

Comprehensive analysis of the long non-coding RNA expression profile and functional roles in a contrast-induced acute kidney injury rat model
Exp Ther Med. 2021 Jul;22(1):739. doi: 10.3892/etm.2021.10171. Epub 2021 May 9.ABSTRACTLong non-coding RNAs (lncRNAs) have been identified as a class of regulatory RNAs that participate in both physiological and pathological conditions, including acute kidney injury. However, the roles of lncRNA dysregulation in the pathogenesis of contrast-induced acute kidney injury (CI-AKI) are largely unknown. In the present study, the expression profiles of lncRNAs in kidney tissue were compared between rats with CI-AKI and controls using high-throughput RNA sequencing. In total, 910 differentially expressed (DE) lncRNAs (DElncRNAs), ...
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Weiwei Bao Zhigang Xiao Zhiqing Wang Donglin Liu Ping Tan Mingfang Huang Source Type: research

Hirsutanol A inhibits T-acute lymphocytic leukemia Jurkat cell viability through cell cycle arrest and p53-dependent induction of apoptosis
Exp Ther Med. 2021 Jul;22(1):741. doi: 10.3892/etm.2021.10173. Epub 2021 May 11.ABSTRACTAcute lymphocytic leukemia (ALL) is a type of childhood leukemia with the highest incidence; T-acute lymphocytic leukemia (T-ALL) is far more difficult to treat than B-acute lymphocytic leukemia (B-ALL) and has a poor long-term prognosis. Therefore, there is an urgent requirement to develop effective drugs for the treatment of T-ALL. Hirsutanol A is a natural sesquiterpenoid compound. The aim of the present study was to evaluate the in vitro anticancer activity of hirsutanol A against T-acute lymphocytic leukemia Jurkat cells and invest...
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Fangfang Zhong You Yang Danwei Ren Sili Long Xiang Qin Jing Liu Yan Zeng Wenjian Lan Wenzhe Ma Wenjun Liu Source Type: research

Thymosin β10 mediates the effects of microRNA-184 in the proliferation and epithelial-mesenchymal transition of BCPAP cells
Exp Ther Med. 2021 Jul;22(1):742. doi: 10.3892/etm.2021.10174. Epub 2021 May 11.ABSTRACTThyroid cancer is the most common malignant tumor of the endocrine system. It has been reported that thymosin β10 (TMSB10) serves a vital role in tumor invasion and metastasis, and further understanding the role of TMSB10 in thyroid cancer may provide new insights into the development of novel targeted drugs. Bioinformatics analysis suggested that there might exist a regulatory relationship between miR-184 and TMSB10. Therefore, the expression of microRNA (miR)-184 was investigated in the TPC-1 and BCPAP thyroid cancer cell lines a...
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Cheng Yang Yunni Liu Kun Fang Source Type: research

Rosiglitazone alleviates lipopolysaccharide-induced inflammation in RAW264.7 cells via inhibition of NF- κB and in a PPARγ-dependent manner
Exp Ther Med. 2021 Jul;22(1):743. doi: 10.3892/etm.2021.10175. Epub 2021 May 11.ABSTRACTRosiglitazone is a synthetic peroxisome proliferator-activated receptor (PPAR)γ agonist widely used for the treatment of type 2 diabetes. Recent studies have demonstrated that rosiglitazone displays anti-inflammatory effects. The present study aimed to investigate whether rosiglitazone alleviates decreases in RAW264.7 cell viability resulting from lipopolysaccharide (LPS)-induced inflammation, as well as exploring the underlying mechanism. A macrophage inflammatory injury model was established by treating RAW264.7 cells with 100 n...
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Jing-Ping Zhou Xiao-Ning Yang Yang Song Fei Zhou Jing-Jing Liu Yi-Qun Hu Li-Gang Chen Source Type: research

miR-489-3p inhibits TLR4/NF- κB signaling to prevent inflammation in psoriasis
Exp Ther Med. 2021 Jul;22(1):744. doi: 10.3892/etm.2021.10176. Epub 2021 May 11.ABSTRACTPsoriasis is a chronic inflammatory skin disease whose etiology has not yet been determined. MicroRNAs (miRs) regulate the early stages of psoriasis and are targets for therapeutic intervention. The present study aimed to investigate the functional role of miR-489-3p in psoriasis. The present study first assessed the expression levels of miR-489-3p and Toll-like receptor (TLR)4 mRNA using reverse transcription-quantitative PCR, and also detected the protein expression levels of TLR4 and NF-κB via western blot analysis. TargetScan ...
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Yujian Ye Ping Wang Fangmei Zhou Source Type: research

Nephrogenic diabetes insipidus in children (Review)
Exp Ther Med. 2021 Jul;22(1):746. doi: 10.3892/etm.2021.10178. Epub 2021 May 11.ABSTRACTNephrogenic diabetes insipidus (NDI) is characterized by impaired urinary concentrating ability, despite normal or elevated plasma concentrations of the antidiuretic hormone, arginine vasopressin (AVP). NDI can be inherited or acquired. NDI can result from genetic abnormalities, such as mutations in the vasopressin V2 receptor (AVPR2) or the aquaporin-2 (AQP2) water channel, or acquired causes, such as chronic lithium therapy. Congenital NDI is a rare condition. Mutations in AVPR2 are responsible for approximately 90% of patients with c...
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Carmen Duicu Ana Maria Pitea Oana Maria S ăsăran Iulia Cozea Lidia Man Claudia B ănescu Source Type: research

Comparative hematological profiles for dose-dense vs. regular anthracycline-based neoadjuvant chemotherapy in non-metastatic breast cancer
Exp Ther Med. 2021 Jul;22(1):747. doi: 10.3892/etm.2021.10179. Epub 2021 May 11.ABSTRACTThe aim of the present study was to examine both the feasibility and toxicity of neoadjuvant dose-dense chemotherapy in women with non-metastatic breast cancer. A search within the OncoHelp Association breast cancer database has been performed in order to identify all non-metastatic breast cancer patients who underwent an initial consultation with a medical oncologist between March 2016 and April 2020. The inclusion criteria used were: i) Age, ii) follow-up care obtained at OncoHelp Association, iii) the intent to treat with a neoadjuva...
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Dorel Popovici Cristina Oprean Sorin S ăftescu Alina Negru Mihnea Munteanu Horia T Stanca Adrian Teodoru Simona Stanca Șerban Negru Source Type: research

Antibiotic resistance patterns of urinary tract pathogens in children from Central Romania
Exp Ther Med. 2021 Jul;22(1):748. doi: 10.3892/etm.2021.10180. Epub 2021 May 12.ABSTRACTOne of the most frequent bacterial infections in children are urinary tract infections (UTIs). In recent years, an increasing incidence of UTIs caused by resistant bacterial strains has been observed, especially with extended-spectrum β-lactamase-producing Enterobacteriaceae that represent about 15% of UTIs. A retrospective study was performed comprising 331 pediatric cases with UTI. Our study aimed to detect the resistance of the uropathogens to common drugs used in UTI treatment. High resistance rates have been recorded for ampic...
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Carmen Duicu Iulia Cozea Dan Delean Andreea Alexandra Aldea Cornel Aldea Source Type: research

Magnetic resonance imaging in schizophrenia: Luxury or necessity? (Review)
Exp Ther Med. 2021 Jul;22(1):765. doi: 10.3892/etm.2021.10197. Epub 2021 May 14.ABSTRACTSchizophrenia, one of the most common psychiatric disorders, with a worldwide annual incidence rate of approximately 0.3-0.7%, known to affect the population below 25 years of age, is persistent throughout lifetime and includes people from all layers of society. With recent technological progress that allows better imaging techniques, such as the ones provided by computed tomography and particularly magnetic resonance imaging (MRI), research on schizophrenia imaging has grown considerably. The purpose of this review is to establish the ...
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Floris Petru Iliuta Mihnea Costin Manea Magdalena Budisteanu Adela Magdalena Ciobanu Mirela Manea Source Type: research

< em > Helicobacter pylori < /em > infection over bile reflux: No influence on the severity of endoscopic or premalignant gastric lesion development
This study investigated the histologic gastric changes in patients with primary DGR and H. pylori infection, as well as their endoscopic findings, symptoms, drug consumption, and social behavior in comparison with patients presenting only DGR. The study included 560 patients with primary DGR on endoscopy divided into two groups, according to the presence/absence of H. pylori infection on biopsy (utilizing usual stainings and immunohistochemical methods). There was no significant difference in terms of age and sex, nor in the frequency of diabetes or esophagitis between the studied groups. Epigastric pain was associated wit...
Source: Experimental and Therapeutic Medicine - May 31, 2021 Category: General Medicine Authors: Andreea Sz őke Simona Mocan Anca Negovan Source Type: research