Three-Dimensional Confocal Analysis of Chromosome Positioning Coupled with Immunofluorescence in Mouse Sperm Nuclei.
We describe here a in situ fluorescence hybridization (FISH) strategy coupled with a fluorescent immunocytochemistry approach followed by confocal analysis and reconstruction (2D/3D) as a powerful tool to analyze the location of chromosomes in the sperm nucleus using the mouse sperm as a model. Already, the two-dimensional (2D) analysis of FISH and immunofluorescence data reveal the location of chromosomes as well as the different markings on the spermatic nucleus. In addition, a good 3D rendering after Imaris software processing was obtained when Z-stacks of images were acquired over a defined volume (10 μm &...
Source: Reproductive Biology - August 23, 2020 Category: Reproduction Medicine Authors: Champroux A, Goubely C, Henry-Berger J, Drevet JR, Kocer A Tags: Methods Mol Biol Source Type: research

Establishment of a WHO Reference Reagent for anti-Mullerian hormone.
CONCLUSION: The majority of methods met the validity criteria. Content estimates showed a high between-method variability, yet assays exhibited a similar proportionality of response as demonstrated using the comparator sample. 16/190 was commutable in some but not all methods. On the basis of these results, it was agreed by the WHO Expert Committee on Biological Standardization to establish 16/190 as a WHO Reference Reagent for AMH with a content defined by consensus immunoassay of 489 ng/ampoule. PMID: 32799874 [PubMed - as supplied by publisher] (Source: Reproductive Biology)
Source: Reproductive Biology - August 15, 2020 Category: Reproduction Medicine Authors: Ferguson J, Hockley J, Rigsby P, Burns C Tags: Reprod Biol Endocrinol Source Type: research

Regulation of [Ca2+]i oscillations and mitochondrial activity by various calcium transporters in mouse oocytes.
Abstract Oocyte activation inefficiency is one of the reasons for female infertility and Ca2+ functions play a critical role in the regulation of oocyte activation. We used various inhibitors of Ca2+ channels located on the membrane, including sarcoplasmic/ endoplasmic reticulum Ca2+ATPases (SERCAs, the main Ca2+ pumps which decrease the intracellular Ca2+ level by refilling Ca2+ into the sarcoplasmic reticulum), transient receptor potential (TRP) ion channel subfamily member 7 (TRPM7, a Ca2+/Mg2+-permeable non-selective cation channel), T-type Ca2+ channels and calcium channel Orai1, to investigate their roles in...
Source: Reproductive Biology - August 15, 2020 Category: Reproduction Medicine Authors: Wang F, Li A, Meng TG, Wang LY, Wang LJ, Hou Y, Schatten H, Sun QY, Ou XH Tags: Reprod Biol Endocrinol Source Type: research

Correlation of low serum vitamin-D with uterine leiomyoma: a systematic review and meta-analysis.
Abstract BACKGROUND: There is a growing body of evidence on low serum vitamin-D levels and the risk of uterine leiomyomas (UL). Therefore, this systematic review and meta-analysis was conducted to investigate the association between serum vitamin D levels and UL occurrence. METHODS: Searches were systematically conducted of the electronic databases PubMed, Scopus, EMBASE, Web of Science (ISI), Cochrane library, Ovid, and Google Scholar to identify relevant studies from inception until February 6, 2020. Heterogeneity across the included studies was examined using Cochran's Q and I-square (I2). Data was pooled ...
Source: Reproductive Biology - August 14, 2020 Category: Reproduction Medicine Authors: Mohammadi R, Tabrizi R, Hessami K, Ashari H, Nowrouzi-Sohrabi P, Hosseini-Bensenjan M, Asadi N Tags: Reprod Biol Endocrinol Source Type: research

Histone acetylation and the role of histone deacetylases in normal cyclic endometrium.
Abstract Histone acetylation is a critical epigenetic modification that changes chromatin architecture and regulates gene expression by opening or closing the chromatin structure. It plays an essential role in cell cycle progression and differentiation. The human endometrium goes through cycles of regeneration, proliferation, differentiation, and degradation each month; each phase requiring strict epigenetic regulation for the proper functioning of the endometrium. Aberrant histone acetylation and alterations in levels of two acetylation modulators - histone acetylases (HATs) and histone deacetylases (HDACs) ...
Source: Reproductive Biology - August 13, 2020 Category: Reproduction Medicine Authors: Gujral P, Mahajan V, Lissaman AC, Ponnampalam AP Tags: Reprod Biol Endocrinol Source Type: research

Effect of transient scrotal hyperthermia on human sperm: an iTRAQ-based proteomic analysis.
CONCLUSIONS: The results indicated that the proteins AKAP4, ODF1, ODF2, GAPDHS, SPESP1, and ACTRT2, play an important role in the heat-induced reversible reduction in sperm concentration and motility and have the potential to be the biomarkers and clinical targets for scrotal heat treatment induced male infertility. PMID: 32787870 [PubMed - as supplied by publisher] (Source: Reproductive Biology)
Source: Reproductive Biology - August 12, 2020 Category: Reproduction Medicine Authors: Wu YQ, Rao M, Hu SF, Ke DD, Zhu CH, Xia W Tags: Reprod Biol Endocrinol Source Type: research

The significance of FMR1 CGG repeats in Chinese women with premature ovarian insufficiency and diminished ovarian reserve.
CONCLUSIONS: The study shows that the frequency of FMR1 premutations is relatively low (1.6%) in Chinese women with POI. The distribution of allele 1 CGG repeat in patients with POI showed difference from that in healthy women. PMID: 32787884 [PubMed - as supplied by publisher] (Source: Reproductive Biology)
Source: Reproductive Biology - August 12, 2020 Category: Reproduction Medicine Authors: Tang R, Yu Q Tags: Reprod Biol Endocrinol Source Type: research

Androgen levels in the fetal cord blood of children born to women with polycystic ovary syndrome: a meta-analysis.
CONCLUSIONS: Fetal cord blood T level is not related to PCOS, while ADION levels tend to be lower in the cord blood of daughters born to mothers with PCOS. PMID: 32782029 [PubMed - in process] (Source: Reproductive Biology)
Source: Reproductive Biology - August 11, 2020 Category: Reproduction Medicine Authors: Duan C, Pei T, Li Y, Cao Q, Chen H, Fu J Tags: Reprod Biol Endocrinol Source Type: research

Pachymic acid protects oocyte by improving the ovarian microenvironment in PCOS mice.
In this study, we generated a PCOS mice model by hypodermic injection of dehydroepiandrosterone (DHEA),and metformin was used as a positive control drug to study the effect of pachymic acid (PA) on endocrine and oocyte quality in PCOS mice.Compared with the model group, the mice treated with PA showed the following changes (slower weight gain, improved abnormal metabolism; increased development potential of GV oocytes, reduced number of abnormal MII oocytes and damaged embryos; lower expression of ovarian-related genes in ovarian tissue and proinflammatory cytokines in adipose tissue). All these aspects show similar effect...
Source: Reproductive Biology - August 10, 2020 Category: Reproduction Medicine Authors: Fu XP, Xu L, Fu BB, Wei KN, Liu Y, Liao BQ, He SW, Wang YL, Chen MH, Lin YH, Li FP, Hong ZW, Huang XH, Xu CL, Wang HL Tags: Biol Reprod Source Type: research

C-ECi: A cubic-ECi combined clearing method for 3D follicular content analysis in The fish ovary †.
C-ECi: A cubic-ECi combined clearing method for 3D follicular content analysis in The fish ovary†. Biol Reprod. 2020 Aug 10;: Authors: Lesage M, Thomas M, Bugeon J, Branthonne A, Gay S, Cardona E, Haghebaert M, Mahé F, Bobe J, Thermes V Abstract Deciphering mechanisms of oocyte development in female fishes still remains challenging and a comprehensive overview of this process at the level of the organ is still needed. The recent development of optical tissue clearing methods has tremendously boosted the 3D imaging of large size biological samples that are naturally opaque. However, no at...
Source: Reproductive Biology - August 10, 2020 Category: Reproduction Medicine Authors: Lesage M, Thomas M, Bugeon J, Branthonne A, Gay S, Cardona E, Haghebaert M, Mahé F, Bobe J, Thermes V Tags: Biol Reprod Source Type: research

Peripheral action of kisspeptin at reproductive tissues-role in ovarian function and embryo implantation and relevance to assisted reproductive technology in livestock: A review.
Abstract Kisspeptin (KISS1) is encoded by the KISS1 gene and was initially found to be a repressor of metastasis. Natural mutations in the KISS1 receptor gene (KISS1R) were subsequently shown be associated with idiopathic hypothalamic hypogonadism and impaired puberty. This led to interest in the role of KISS1 in reproduction. It was established that KISS1 had a fundamental role in the control of GnRH secretion. KISS1 neurons have receptors for leptin and estrogen receptor α (ERα) which places KISS1 at the gateway of metabolic (leptin) and gonadal (ERα) regulation of GnRH secretion. More recently...
Source: Reproductive Biology - August 10, 2020 Category: Reproduction Medicine Authors: D'Occhio MJ, Campanile G, Baruselli PS Tags: Biol Reprod Source Type: research

In Utero Low Protein Diet Programmed Type 2 Diabetes in adult offspring is mediated by Sex Hormones in Rats †.
In Utero Low Protein Diet Programmed Type 2 Diabetes in adult offspring is mediated by Sex Hormones in Rats†. Biol Reprod. 2020 Aug 07;: Authors: Blesson CS, Schutt AK, Vipin VA, Tanchico DT, Mathew PR, Balakrishnan M, Bethancourt A, Yallampalli C Abstract Sex steroids regulate insulin sensitivity and glucose metabolism. We had characterized a lean type 2 diabetes (T2D) rat model using gestational low protein (LP) diet programming. Our objective was to identify if endocrine dysfunction leading to decreased sex hormone levels will precede the development of T2D and if steroid replacement will pr...
Source: Reproductive Biology - August 7, 2020 Category: Reproduction Medicine Authors: Blesson CS, Schutt AK, Vipin VA, Tanchico DT, Mathew PR, Balakrishnan M, Bethancourt A, Yallampalli C Tags: Biol Reprod Source Type: research

Zika virus in rhesus macaque semen and reproductive tract tissues: a pilot study of acute infection †.
Zika virus in rhesus macaque semen and reproductive tract tissues: a pilot study of acute infection†. Biol Reprod. 2020 Aug 06;: Authors: Kropp Schmidt JA, Mean KD, Puntney RC, Alexander ES, Sullivan R, Simmons HA, Zeng X, Weiler AM, Friedrich TC, Golos TG Abstract Although sexual transmission of Zika virus (ZIKV) is well-documented, the viral reservoir(s) in the male reproductive tract remains uncertain in humans and immune-intact animal models. We evaluated the presence of ZIKV in a rhesus macaque pilot study to determine persistence in semen, assess the impact of infection on sperm functiona...
Source: Reproductive Biology - August 6, 2020 Category: Reproduction Medicine Authors: Kropp Schmidt JA, Mean KD, Puntney RC, Alexander ES, Sullivan R, Simmons HA, Zeng X, Weiler AM, Friedrich TC, Golos TG Tags: Biol Reprod Source Type: research

Corrigendum: Experimentally Induced Endometritis Impairs the Developmental Capacity of Bovine Oocytes †.
Corrigendum: Experimentally Induced Endometritis Impairs the Developmental Capacity of Bovine Oocytes†. Biol Reprod. 2020 Aug 06;: Authors: Dickson MJ, Piersanti RL, Ramirez-Hernandez R, de Oliveira EB, Bishop JV, Hansen TR, Ma Z, Jeong KCC, Santos JEP, Sheldon MI, Block J, Bromfield JJ PMID: 32761062 [PubMed - as supplied by publisher] (Source: Reproductive Biology)
Source: Reproductive Biology - August 6, 2020 Category: Reproduction Medicine Authors: Dickson MJ, Piersanti RL, Ramirez-Hernandez R, de Oliveira EB, Bishop JV, Hansen TR, Ma Z, Jeong KCC, Santos JEP, Sheldon MI, Block J, Bromfield JJ Tags: Biol Reprod Source Type: research

Efficient generation of bone morphogenetic protein 15-edited Yorkshire pigs using CRISPR/Cas9 †.
Efficient generation of bone morphogenetic protein 15-edited Yorkshire pigs using CRISPR/Cas9†. Biol Reprod. 2020 Aug 06;: Authors: Shi X, Tang T, Lin Q, Liu H, Qin Y, Liang X, Cong P, Mo D, Liu X, Chen Y, He Z Abstract Bone morphogenetic protein 15 (BMP15), a member of the transforming growth factor beta (TGF-β) superfamily, plays an essential role in ovarian follicular development in mono-ovulatory mammalian species. Studies using a biallelic knockout mouse model revealed BMP15 potentially has just a minimal impact on female fertility and ovarian follicular development in poly-ovulatory ...
Source: Reproductive Biology - August 6, 2020 Category: Reproduction Medicine Authors: Shi X, Tang T, Lin Q, Liu H, Qin Y, Liang X, Cong P, Mo D, Liu X, Chen Y, He Z Tags: Biol Reprod Source Type: research

The Enigmatic Sperm Proteins in Mammalian Fertilization: An Overview.
Abstract Mammalian fertilization involves a physical interaction between a sperm and an egg followed by molecular interactions amongst their various cell surface molecules. These interactions are initially mediated on the egg's outermost matrix, zona pellucida (ZP), and then its plasma membrane. To better understand this process, it is pertinent to find the corresponding molecules on sperm that interact with ZP or the egg's plasma membrane. Although currently, we have some knowledge about the binding partners for egg's plasma membrane on sperm, yet the ones involved in an interaction with ZP have remained remarkab...
Source: Reproductive Biology - August 6, 2020 Category: Reproduction Medicine Authors: Gahlay GK, Rajput N Tags: Biol Reprod Source Type: research

Age-related alterations in fertilization-induced Ca2+ oscillations depend on the genetic background of mouse oocytes.
We examined three different mouse strains/crosses: inbred C57BL/6Tar, outbred Tar:SWISS, and hybrid F1 (C57BL/6Tar x CBA/Tar). The females were either 2-4 months old (young) or 13-16 months old (aged). We observed that Ca2+ oscillatory pattern is altered in a strain-dependent manner and that changes were more profound in aged C57BL/6Tar and F1 than in aged Tar:SWISS oocytes. We also showed that maternal aging differently affects the size of Ca2+ store and expression of Itpr, Atp2a2, Txndc4 and Pdia3 genes involved in Ca2+ homeostasis in oocytes of C57BL/6Tar, Tar:SWISS and F1 genetic background, which may explain...
Source: Reproductive Biology - August 6, 2020 Category: Reproduction Medicine Authors: Czajkowska K, Walewska A, Ishikawa T, Szczepańska K, Ajduk A Tags: Biol Reprod Source Type: research

Influence of human chorionic gonadotrophin during ovarian stimulation: an overview.
Abstract It is widely known that luteinising hormone (LH) and human chorionic gonadotrophin (hCG) are integral in the female reproductive lifecycle. Due to the common binding site and similarity in molecular structure, they were previously thought to have overlapping roles. However, with the development of both purified urinary-derived and recombinant gonadotrophins, the individual characteristics of these molecules have begun to be defined. There is evidence to suggest that LH and hCG preferentially activate different signalling cascades and display different receptor-binding kinetics. The data generated on the t...
Source: Reproductive Biology - August 6, 2020 Category: Reproduction Medicine Authors: Smitz J, Platteau P Tags: Reprod Biol Endocrinol Source Type: research

A new oocyte-holding pipette for intracytoplasmic sperm injection without cytoplasmic aspiration: An experimental study in mouse oocytes.
e;ndez A, Ponce J Abstract We assessed the feasibility of using a new oocyte-holding pipette (pipette without aspiration, PiWA) for intracytoplasmic sperm injection (ICSI), which prevents cytoplasmic aspiration during microinjection. A pilot experimental study in eight mature mouse oocytes to assess the feasibility of the oocyte-holding PiWA for ICSI procedure. The absence of oocyte degeneration after microinjection and the viability of correct embryo development were also evaluated. The pipette comprises a suction conduit inside an elongated cylindrical body and a funnel-shaped working end, which is dimensioned t...
Source: Reproductive Biology - August 6, 2020 Category: Reproduction Medicine Authors: Fernández J, Pedrosa C, Vergara F, Nieto AI, Quintas A, Fernández A, Moyano C, Fernández A, Ponce J Tags: Reprod Biol Source Type: research

Intra-ovarian injection of platelet-rich plasma into ovarian tissue promoted rejuvenation in the rat model of premature ovarian insufficiency and restored ovulation rate via angiogenesis modulation.
Abstract Premature Ovarian Insufficiency (POI) is viewed as a type of infertility in which the menopausal status occurs before the physiological age. Several therapeutic strategies have been introduced in clinic for POI treatment, although the outputs are not fully convincing. Platelet-rich plasma (PRP) is a unique blood product widely applied in regenerative medicine, which is based on the releasing of the growth factors present in platelets α-granules. In the current investigation, we examined the effectiveness of PRP as a therapeutic alternative for POI animals. POI in Wistar albino rats was induced by da...
Source: Reproductive Biology - August 5, 2020 Category: Reproduction Medicine Authors: Ahmadian S, Sheshpari S, Pazhang M, Bedate AM, Beheshti R, Abbasi MM, Nouri M, Rahbarghazi R, Mahdipour M Tags: Reprod Biol Endocrinol Source Type: research

Clinical outcomes of Preimplantation genetic testing (PGT) application in couples with chromosomal inversion, a study in the Chinese Han population.
CONCLUSIONS: Due to limitation of retrospective study and small sample size, our current data showed that PGT cannot provide prominent benefits for inversion carriers in the Chinese Han population. Further prospective randomized controlled trials are needed to evaluate the effects of PGT. PMID: 32758287 [PubMed - in process] (Source: Reproductive Biology)
Source: Reproductive Biology - August 5, 2020 Category: Reproduction Medicine Authors: Shao Y, Li J, Lu J, Li H, Zhu Y, Jiang W, Yan J Tags: Reprod Biol Endocrinol Source Type: research

Comparative DNA methylome analysis of estrus ewes reveals the complex regulatory pathways of sheep fecundity.
CONCLUSIONS: These findings provide prospective insights on the epigenetic basis of sheep fecundity. PMID: 32753034 [PubMed - in process] (Source: Reproductive Biology)
Source: Reproductive Biology - August 4, 2020 Category: Reproduction Medicine Authors: Miao X, Luo Q, Xie L, Zhao H, Qin X Tags: Reprod Biol Endocrinol Source Type: research

Target Product Profile for an endometrial receptivity test: women's perspective.
CONCLUSIONS: Women understand the important role the endometrium plays for a successful pregnancy and they have specific views in relation to the indication, timing and invasiveness of testing, need for test repetition, validity of results and costs of testing. PMID: 32771887 [PubMed - as supplied by publisher] (Source: Reproductive Biology)
Source: Reproductive Biology - August 1, 2020 Category: Reproduction Medicine Authors: Craciunas L, Pickering O, Chu J, Žurauskienė J, Coomarasamy A Tags: Eur J Obstet Gynecol Reprod Biol Source Type: research

Being an obstetrics and gynaecology resident during the COVID-19: Impact of the pandemic on the residency training program.
CONCLUSIONS: Among Italian residents in obstetrics and gynecology, COVID-19 pandemic was associated with a significant training impairment. PMID: 32771888 [PubMed - as supplied by publisher] (Source: Reproductive Biology)
Source: Reproductive Biology - August 1, 2020 Category: Reproduction Medicine Authors: Bitonti G, Palumbo AR, Gallo C, Rania E, Saccone G, De Vivo V, Zullo F, Di Carlo C, Venturella R Tags: Eur J Obstet Gynecol Reprod Biol Source Type: research

Can a health care worker have sex in the time of COVID-19?
Abstract Covid-19 is not transmitted by sex but close proximity during the act could aide in spread of the disease. Health care workers by virtue of the nature of their work have higher chances of exposure to the virus and them indulging in sex needs risk reduction strategies. PMID: 32778481 [PubMed - as supplied by publisher] (Source: Reproductive Biology)
Source: Reproductive Biology - August 1, 2020 Category: Reproduction Medicine Authors: Naik BS Tags: Eur J Obstet Gynecol Reprod Biol Source Type: research

Is termination of early pregnancy indicated in women with COVID-19?
PMID: 32456886 [PubMed - indexed for MEDLINE] (Source: Reproductive Biology)
Source: Reproductive Biology - July 22, 2020 Category: Reproduction Medicine Authors: Wu YT, Li C, Zhang CJ, Huang HF, FRCOG Tags: Eur J Obstet Gynecol Reprod Biol Source Type: research

COVID-19 infection during the third trimester of pregnancy: Current clinical dilemmas.
PMID: 32505514 [PubMed - indexed for MEDLINE] (Source: Reproductive Biology)
Source: Reproductive Biology - July 22, 2020 Category: Reproduction Medicine Authors: Fontanella F, Hannes S, Keating N, Martyn F, Browne I, Briet J, McAuliffe FM, Baalman JH Tags: Eur J Obstet Gynecol Reprod Biol Source Type: research

Use of 4 robotic arms performing Senhance ® robotic surgery may reduce the risk of coronavirus infection to medical professionals during COVID-19.
Use of 4 robotic arms performing Senhance® robotic surgery may reduce the risk of coronavirus infection to medical professionals during COVID-19. Eur J Obstet Gynecol Reprod Biol. 2020 Aug;251:274-275 Authors: Samalavicius NE, Siaulys R, Janusonis V, Klimasauskiene V, Dulskas A PMID: 32536465 [PubMed - indexed for MEDLINE] (Source: Reproductive Biology)
Source: Reproductive Biology - July 22, 2020 Category: Reproduction Medicine Authors: Samalavicius NE, Siaulys R, Janusonis V, Klimasauskiene V, Dulskas A Tags: Eur J Obstet Gynecol Reprod Biol Source Type: research

A practical approach for the management of obstetric and infertile women during the phase two of the novel coronavirus disease 2019 (COVID -19) pandemic.
PMID: 32536467 [PubMed - indexed for MEDLINE] (Source: Reproductive Biology)
Source: Reproductive Biology - July 22, 2020 Category: Reproduction Medicine Authors: Carbone IF, Conforti A, Farina A, Alviggi C Tags: Eur J Obstet Gynecol Reprod Biol Source Type: research

Covid-19 in pregnant women: General data from a French National Survey.
PMID: 32540156 [PubMed - indexed for MEDLINE] (Source: Reproductive Biology)
Source: Reproductive Biology - July 22, 2020 Category: Reproduction Medicine Authors: Cohen J, Vignaux O, Jacquemard F Tags: Eur J Obstet Gynecol Reprod Biol Source Type: research

UXT in Sertoli Cells is Required for Blood-Testis Barrier Integrity.
Abstract Spermatogenesis is a complex process that establishes male fertility and involves proper communication between the germline (spermatozoa) and the somatic tissue (Sertoli cells). Many factors that are important for spermatozoa production are also required for Sertoli cell function. Recently, we showed that the transcriptional co-factor Ubiquitously Expressed Transcript (UXT) encodes a protein that is essential in germ cells for spermatogenesis and fertility. However, the role of UXT within Sertoli cells and how it affects Sertoli cell function was still unclear. Here we describe a novel role for UXT in the...
Source: Reproductive Biology - July 17, 2020 Category: Reproduction Medicine Authors: Thomas PA, Schafler ED, Ruff SE, Voisin M, Ha S, Logan SK Tags: Biol Reprod Source Type: research

Filamentous actin disorganization and absence of apical ectoplasmic specialization disassembly during spermiation upon interference with retinoid signaling.
Abstract Spermiation is a multiple-step process involving profound cellular changes in both spermatids and Sertoli cells. We have observed spermiation defects, including abnormalities in spermatid orientation, translocation and release, in mice deficient in the retinoic acid receptor alpha (RARA) and upon treatment with a pan-RAR antagonist. To elucidate the role of retinoid signaling in regulating spermiation, we first characterized the time course of appearance of spermiogenic defects in response to treatment with the pan-RAR antagonist. The results revealed that defects in spermiation are indeed among the earli...
Source: Reproductive Biology - July 17, 2020 Category: Reproduction Medicine Authors: Chung SSW, Vizcarra N, Wolgemuth DJ Tags: Biol Reprod Source Type: research

Analysis of expression of candidate genes for polycystic ovary syndrome in adult and fetal human and fetal bovine ovaries †.
Analysis of expression of candidate genes for polycystic ovary syndrome in adult and fetal human and fetal bovine ovaries†. Biol Reprod. 2020 Jul 17;: Authors: Liu M, Hummitzsch K, Hartanti MD, Rosario R, Bastian NA, Hatzirodos N, Bonner WM, Irving-Rodgers HF, Laven JSE, Anderson RA, Rodgers RJ Abstract Polycystic ovary syndrome (PCOS) appears to have a genetic predisposition and a fetal origin. We compared the expression levels of 25 PCOS candidate genes from adult control and PCOS human ovaries (n = 16) using microarrays. Only one gene was potentially statistically different. Using ...
Source: Reproductive Biology - July 17, 2020 Category: Reproduction Medicine Authors: Liu M, Hummitzsch K, Hartanti MD, Rosario R, Bastian NA, Hatzirodos N, Bonner WM, Irving-Rodgers HF, Laven JSE, Anderson RA, Rodgers RJ Tags: Biol Reprod Source Type: research

Does the sex ratio of singleton births after frozen single blastocyst transfer differ in relation to blastocyst development?
Abstract PURPOSE: To investigate the associations between blastocyst development and the sex ratio (male:female) among singleton live births resulting from single-blastocyst frozen embryo transfer (FET) cycles. METHODS: Patients with singleton live births following the first autologous single FET of non- preimplantation genetic testing (PGT) blastocysts in a single reproductive medicine department between January 2015 and February 2019 were included in this retrospective study. The primary outcome measure was the singleton sex ratio. Multivariable logistic regression models were used to estimate the associati...
Source: Reproductive Biology - July 15, 2020 Category: Reproduction Medicine Authors: Lou H, Li N, Zhang X, Sun L, Wang X, Hao D, Cui S Tags: Reprod Biol Endocrinol Source Type: research

Gonadotropin-releasing hormone agonist combined with hormone replacement therapy does not improve the reproductive outcomes of frozen-thawed embryo transfer cycle in elderly patients: a retrospective study.
CONCLUSION: A GnRH agonist combined with hormone replacement therapy did not improve the reproductive outcomes of frozen-thawed embryo cycles in elderly patients. PMID: 32669123 [PubMed - in process] (Source: Reproductive Biology)
Source: Reproductive Biology - July 15, 2020 Category: Reproduction Medicine Authors: Dong M, Sun L, Huang L, Yi Y, Zhang X, Tan Y, Song G, Liu L, Wei F, Liu F Tags: Reprod Biol Endocrinol Source Type: research

Association between mild stimulated IVF/M  cycle and early embryo arrest in sub fertile women with/without PCOS.
In this study, we analyzed the clinical and laboratory outcomes of an optimized IVM protocol in patients with and without PCOS. We also discussed the possible reasons for early embryo arrest in the IVM cycle. METHODS: This prospective study involved 58 PCOS patients and 56 non-PCOS patients who underwent mild stimulated IVF combined IVM (IVF/M) cycles. The clinical and laboratory outcomes were compared between the two groups. Also, metaphase II (MII) oocytes were obtained after IVM from the two groups, and in vivo MII oocytes randomly collected from IVF patients were examined for mitochondrial function using a laser s...
Source: Reproductive Biology - July 15, 2020 Category: Reproduction Medicine Authors: Elshewy N, Ji D, Zhang Z, Chen D, Chen B, Xue R, Wu H, Wang J, Zhou P, Cao Y Tags: Reprod Biol Endocrinol Source Type: research

Down regulating PHGDH affects the lactate production of sertoli cells in varicocele.
CONCLUSIONS: Overall, we conformed that varicocele lead to the decreasing of testis lactate production. Down-regulation of PHGDH in sertoli cells may mediate the process of abnormal glucose metabolism. Our study provide new insight into the mechanisms underlying metabolism-associated male infertility and suggests a novel therapeutic target for male infertility. PMID: 32664979 [PubMed - in process] (Source: Reproductive Biology)
Source: Reproductive Biology - July 14, 2020 Category: Reproduction Medicine Authors: Guo WB, Huang ZH, Yang C, Lv XY, Xia H, Tian H, Yang JK, Zhou QZ, Chen MK, Xue KY, Liu CD Tags: Reprod Biol Endocrinol Source Type: research

Changes in peripheral mitochondrial DNA copy number in metformin-treated women with polycystic ovary syndrome: a longitudinal study.
CONCLUSIONS: Treatment with metformin is associated with a time-dependent decrease in mtDNA-CN in patients with PCOS who are treated over the course of one year. This may signify a reduction in mitochondria dysfunction. The change in mtDNA-CN corresponds to a similar change in serum total testosterone, and suggests a possible relationship between mtDNA-CN and testosterone. TRIAL REGISTRATION: ClinicalTrials.gov , NCT00172523 . Registered September 15, 2005. PMID: 32660613 [PubMed - in process] (Source: Reproductive Biology)
Source: Reproductive Biology - July 13, 2020 Category: Reproduction Medicine Authors: Yang PK, Chou CH, Chang CH, Chen SU, Ho HN, Chen MJ Tags: Reprod Biol Endocrinol Source Type: research

Number of embryos transferred and diagnosis of preeclampsia.
CONCLUSIONS: Among ART births, the transfer of more than 1 embryo for singleton gestations and more than 2 embryos for twin gestations increased the risk for preeclampsia diagnosis. PMID: 32653045 [PubMed - in process] (Source: Reproductive Biology)
Source: Reproductive Biology - July 11, 2020 Category: Reproduction Medicine Authors: Sites CK, Wilson D, Bernson D, Boulet S, Zhang Y Tags: Reprod Biol Endocrinol Source Type: research

Corifollitropin alfa for poor responders patients, a prospective randomized study.
Abstract BACKGROUND: Poor ovarian response remains one of the biggest challenges for reproductive endocrinologists. The introduction of corifollitropin alpha (CFA) offered an alternative option to other gonadotropins for its longer half-life, its more rapid achievement of the threshold and higher FSH levels. We compared two different protocols with CFA, a long agonist and a short antagonist, and a no-CFA protocol. METHODS: Patients enrolled fulfilled at least two of the followings: AFC
Source: Reproductive Biology - July 9, 2020 Category: Reproduction Medicine Authors: Fusi FM, Zanga L, Arnoldi M, Melis S, Cappato M, Candeloro I, Di Pasqua A Tags: Reprod Biol Endocrinol Source Type: research

Oocyte donor's emotions towards the potential offspring: Development and psychometric properties.
CONCLUSION: The 12-item questionnaire is a valid tool for assessing the emotions of oocyte donors shortly after doing donor egg programs. PMID: 32652481 [PubMed - as supplied by publisher] (Source: Reproductive Biology)
Source: Reproductive Biology - July 3, 2020 Category: Reproduction Medicine Authors: Khosravi S, Kazemi A, Ahmadi SM Tags: Eur J Obstet Gynecol Reprod Biol Source Type: research

Human papillomavirus genotyping on Reunion Island: A cross-sectional study of stored tissue samples.
CONCLUSIONS: Immunization with 9-valent vaccine should be effective, and in the long term, may reduce cervical cancer incidence in Reunion Island. Nevertheless, vaccination rates and coverage need to be maintained and improved. PMID: 32650188 [PubMed - as supplied by publisher] (Source: Reproductive Biology)
Source: Reproductive Biology - July 2, 2020 Category: Reproduction Medicine Authors: Tran PL, Zafindraibe N, Ah-You N, Fernandez C, Arrivets P, Gérardin P, Michault A, Boukerrou M, Bertolotti A Tags: Eur J Obstet Gynecol Reprod Biol Source Type: research

Ovarian reserve as a predictor of cumulative live birth.
Abstract OBJECTIVES: Develop an up to date prediction model using recent cycle data and key pre-treatment predictor variables to estimate a couple's individualised probability of a cumulative live birth after one cycle of ovarian stimulation and transfer of all frozen embryos, before the first embryo transfer. STUDY DESIGN: This was a retrospective cohort study. To estimate the cumulative live birth rate we only included couples who had used all embryos from their initial stimulation or achieved a live birth. We constructed a logistic regression model using live birth as a dependent variable and age group, du...
Source: Reproductive Biology - July 2, 2020 Category: Reproduction Medicine Authors: Balachandren N, Salman M, Diu NL, Schwab S, Rajah K, Mavrelos D Tags: Eur J Obstet Gynecol Reprod Biol Source Type: research

Fetal heart rate changes on the cardiotocograph trace secondary to maternal COVID-19 infection.
CONCLUSION: Fetuses of COVID-19 patients showed a raised baseline FHR (>10 percent), loss of accelerations, late decelerations, ZigZag pattern and absence of cycling probably due to the effects of maternal pyrexia, maternal inflammatory response and the "cytokine storm". However, the perinatal outcomes appear to be favourable. Therefore, healthcare providers should optimise the maternal environment first to rectify the reactive CTG changes instead of performing an urgent operative intervention. PMID: 32645644 [PubMed - as supplied by publisher] (Source: Reproductive Biology)
Source: Reproductive Biology - July 2, 2020 Category: Reproduction Medicine Authors: Gracia-Perez-Bonfils A, Martinez-Perez O, Llurba E, Chandraharan E Tags: Eur J Obstet Gynecol Reprod Biol Source Type: research

Expression and localisation of ephrin-B1, EphB2, and EphB4 in the mouse testis during postnatal development.
This study is the first to investigate the expression of ephrin-B1, EphB2, and EphB4 in normal mouse testes during postnatal development. The expression patterns of ephrin-B and EphBs may represent suitable tools for examining organisation of the excurrent duct system and monitoring reproductive toxicity during postnatal development. PMID: 32620380 [PubMed - as supplied by publisher] (Source: Reproductive Biology)
Source: Reproductive Biology - June 30, 2020 Category: Reproduction Medicine Authors: Gofur MR, Alam J, Ogawa K Tags: Reprod Biol Source Type: research

Characterization of the role for cadherin 6 in the regulation of human endometrial receptivity.
This study aimed to investigate whether CDH6 is dysregulated in the endometrium of women with infertility during the receptive window and the effect of CDH6 on endometrial adhesion and receptivity. METHODS: The expression and the localization of CDH6 in the human endometrium were determined by immunohistochemistry. Ishikawa cells were used to investigate the functional consequences of CDH6 knockdown on endometrial adhesive capacity to HTR8/SVneo (trophoblast cell line) spheroids in vitro. CDH6 knockdown was assessed by qPCR and immunoblotting. After CDH6 knockdown, the expression of type II cadherin family members and...
Source: Reproductive Biology - June 29, 2020 Category: Reproduction Medicine Authors: Zhou W, Santos L, Dimitriadis E Tags: Reprod Biol Endocrinol Source Type: research

Ageing in Human Parturition: Impetus of the Gestation Clock in the Decidua.
Abstract Despite sharing many common features, the relationship between ageing and parturition remains poorly understood. The decidua is a specialized lining of endometrial tissue, which develops in preparation for pregnancy. The structure and location of the decidua supports its role as the physical scaffold for the growing embryo and placenta, and thus it is vital to sustain pregnancy. Approaching term, the physical support properties of the decidua are naturally weakened to permit parturition. In this review, we hypothesize that the natural weakening of decidual tissue at parturition is promoted by the ageing p...
Source: Reproductive Biology - June 26, 2020 Category: Reproduction Medicine Authors: Wijaya JC, Khanabdali R, Georgiou HM, Kalionis B Tags: Biol Reprod Source Type: research

Quercetin supplementation to the thawing and incubation media of boar sperm improves post-thaw sperm characteristics and the in vitro production of pig embryos.
The objective of this study was to determine the effects of supplementing quercetin (0.25, 0.50, 0.75 mM) during the thawing and incubation of frozen-thawed boar semen on spermatozoan characteristics, IVF kinetics (n = 400) and subsequent embryonic development (n = 1340). Spermatozoa were evaluated for motility, viability, and membrane lipid peroxidation levels at 0, 2, 4, 6, 8, and 10 h after thawing. Embryos were evaluated for IVF kinetics 12 h after IVF (penetration, polyspermy, male pronucleus formation, IVF efficiency) and cleavage and blastocyst formation at 48 h and 144 h after IVF, respectively. Spermatozoa supplem...
Source: Reproductive Biology - June 22, 2020 Category: Reproduction Medicine Authors: Winn E, Whitaker BD Tags: Reprod Biol Source Type: research

Evaluation of the protective effects of icariin on nicotine-induced reproductive toxicity in male mouse -a pilot study.
CONCLUSIONS: These findings indicate that nicotine-induced reproductive toxicity and oxidative damage on male reproductive tissues could be attenuated by ICA. PMID: 32552695 [PubMed - in process] (Source: Reproductive Biology)
Source: Reproductive Biology - June 18, 2020 Category: Reproduction Medicine Authors: Ni G, Zhang X, Afedo SY, Rui R Tags: Reprod Biol Endocrinol Source Type: research

Organoid technology in female reproductive biomedicine.
Abstract Recent developments in organoid technology are revolutionizing our knowledge about the biology, physiology, and function of various organs. Female reproductive biology and medicine also benefit from this technology. Organoids recapitulate features of different reproductive organs including the uterus, fallopian tubes, and ovaries, as well as trophoblasts. The genetic stability of organoids and long-lasting commitment to their tissue of origin during long-term culture makes them attractive substitutes for animal and in vitro models. Despite current limitations, organoids offer a promising platform to addre...
Source: Reproductive Biology - June 18, 2020 Category: Reproduction Medicine Authors: Heidari-Khoei H, Esfandiari F, Hajari MA, Ghorbaninejad Z, Piryaei A, Baharvand H Tags: Reprod Biol Endocrinol Source Type: research