Low doses of Bisphenol S affect post-translational modifications of sperm proteins in male mice.
CONCLUSIONS: In summary, this range of qualitative and quantitative findings in young male mice raise the possibility that very low doses of BPS may impair mammalian reproduction through epigenetic modifications of sperm proteins. PMID: 32466766 [PubMed - in process] (Source: Reproductive Biology)
Source: Reproductive Biology - May 28, 2020 Category: Reproduction Medicine Authors: Řimnáčová H, Štiavnická M, Moravec J, Chemek M, Kolinko Y, García-Álvarez O, Mouton PR, Trejo AMC, Fenclová T, Eretová N, Hošek P, Klein P, Králíčková M, Petr J, Nevoral J Tags: Reprod Biol Endocrinol Source Type: research

Cigarette smoking is associated with high level of ferroptosis in seminal plasma and affects semen quality.
In this study, semen samples from 70 heavy smokers and 75 non-smokers receiving infertility treatment were included. Basic semen parameters in non-smokers and heavy smokers were evaluated. Levels of glutathione (GSH), lipid reactive oxygen species (ROS), iron and GSH-dependent peroxidase 4 (GPX4) protein level were observed in human seminal plasma and in GC-2Spd cells exposed to cigarette smoke condensate (CSC). RESULTS: Heavy smokers had significantly higher abnormalities (sperm viability and sperm progressive motility) than non-smoking counterparts. Comparing non-smokers group, GSH level was reduced in the group of ...
Source: Reproductive Biology - May 27, 2020 Category: Reproduction Medicine Authors: Ou Z, Wen Q, Deng Y, Yu Y, Chen Z, Sun L Tags: Reprod Biol Endocrinol Source Type: research

Effectiveness and safety of follitropin alfa (Ovaleap ®) for ovarian stimulation using a GnRH antagonist protocol in real-world clinical practice: a multicenter, prospective, open, non-interventional assisted reproductive technology study.
The objective of this study was to examine the effectiveness and safety of ovarian stimulation (OS) with follitropin alfa (Ovaleap®) for routine IVF or intracytoplasmic sperm injection treatment in gonadotropin-releasing hormone (GnRH) antagonist cycles in real-world ART clinical practice. METHODS: This non-interventional, multicenter, prospective study was initiated in 34 specialized reproductive medicine centers in Germany. Eligible women were 18-40 years old with a body mass index
Source: Reproductive Biology - May 26, 2020 Category: Reproduction Medicine Authors: Sydow P, Gmeinwieser N, Pribbernow K, Keck C, Wiegratz I Tags: Reprod Biol Endocrinol Source Type: research

Expression of Concern to: The role of ACE2, angiotensin-(1-7) and Mas1 receptor axis in glucocorticoid-induced intrauterine growth restriction.
Abstract An amendment to this paper has been published and can be accessed via the original article. PMID: 32450887 [PubMed - in process] (Source: Reproductive Biology)
Source: Reproductive Biology - May 25, 2020 Category: Reproduction Medicine Authors: Ghadhanfar E, Alsalem A, Al-Kandari S, Naser J, Babiker F, Al-Bader M Tags: Reprod Biol Endocrinol Source Type: research

Intramuscular injection of human chorionic gonadotropin prior to secretory transformation in patients undergoing frozen-thawed embryo transfer cycles.
Abstract BACKGROUND: The major difference between a natural cycle and an artificially prepared cycle is the lack of luteinizing hormone (LH) peak in the latter. The LH/hCG receptors were identified to express in human endometrium and evidences of experiments also suggested the beneficial role of hCG in embryo implantation, indicating that the LH peak might be of clinical significance and the activation of LH/hCG receptors in the endometrium could improve embryo implantation. Hence, we postulated that the addition of hCG prior to secretory transformation in an artificial cycle might improve pregnancy outcomes. ...
Source: Reproductive Biology - May 25, 2020 Category: Reproduction Medicine Authors: Deng L, Chen X, Blockeel C, Ye DS, Chen SL Tags: Reprod Biol Endocrinol Source Type: research

In Vitro Methods to Simulate Pollution and Photo-Pollution Exposure in Human Skin Epidermis.
Abstract Tissue homeostasis of an individual is a finely orchestrated phenomenon that ensures integrity and steady state in health. Emerging evidence indicates that the environment, especially ambient air pollution, has a lasting impact on this equilibrium (Beelen et al., Lancet 383:785-795, 2014). Environmental pollution consists of diverse entities, namely, particulate matter (PM 2.5, PM 10), ozone, and UV rays, among others (Heroux et al., Int J Public Health 60:619-627, 2015). Understandably, skin epidermis is the first and the most exposed tissue to such a wide range of substances and bears the assault. Previ...
Source: Reproductive Biology - May 24, 2020 Category: Reproduction Medicine Authors: Misra N, Marrot L Tags: Methods Mol Biol Source Type: research

Downregulation of hsa_circ_0005243 induces trophoblast cell dysfunction and inflammation via the β-catenin and NF-κB pathways.
CONCLUSIONS: Downregulation of hsa_circ_0005243 may be associated with the pathogenesis of GDM via the regulation of β-catenin and NF-κB signal pathways, suggesting a new potential therapeutic target for GDM. PMID: 32434530 [PubMed - in process] (Source: Reproductive Biology)
Source: Reproductive Biology - May 20, 2020 Category: Reproduction Medicine Authors: Wang H, Zhou W, She G, Yu B, Sun L Tags: Reprod Biol Endocrinol Source Type: research

Ulipristal acetate before in vitro fertilization: efficacy in infertile women with submucous fibroids.
CONCLUSION: Restoration of normal uterine cavity by UPA treatment prior to IVF treatment avoids surgery and establishes a pregnancy rate comparable to a control group without fibroids. PMID: 32430027 [PubMed - in process] (Source: Reproductive Biology)
Source: Reproductive Biology - May 19, 2020 Category: Reproduction Medicine Authors: Morgante G, Centini G, Troìa L, Orvieto R, De Leo V Tags: Reprod Biol Endocrinol Source Type: research

Prolonged viral persistence in COVID-19 second trimester pregnant patient.
PMID: 32425299 [PubMed - as supplied by publisher] (Source: Reproductive Biology)
Source: Reproductive Biology - May 18, 2020 Category: Reproduction Medicine Authors: Panichaya P, Thaweerat W, Uthaisan J Tags: Eur J Obstet Gynecol Reprod Biol Source Type: research

SARS-CoV-2 infection in very preterm pregnancy: experiences from two cases.
PMID: 32425297 [PubMed - as supplied by publisher] (Source: Reproductive Biology)
Source: Reproductive Biology - May 15, 2020 Category: Reproduction Medicine Authors: Cooke WR, Billett A, Gleeson S, Jacques A, Place K, Siddall J, Walden A, Soulsby K Tags: Eur J Obstet Gynecol Reprod Biol Source Type: research

Covid-19 during pregnancy: a case series from an universally tested population from the north of Portugal.
M, Silva PT PMID: 32425298 [PubMed - as supplied by publisher] (Source: Reproductive Biology)
Source: Reproductive Biology - May 15, 2020 Category: Reproduction Medicine Authors: Dória M, Peixinho C, Laranjo M, Varejão AM, Silva PT Tags: Eur J Obstet Gynecol Reprod Biol Source Type: research

Carbon monoxide increases utero-placental angiogenesis without impacting pregnancy specific adaptations in mice.
CONCLUSIONS: This study suggests that CO exposure is pro-angiogenic at the maternal-fetal interface, and is not associated with demonstrable concerns during murine pregnancy. Future studies are required to validate safety and efficacy of CO as a potential therapeutic for vascular insufficiency diseases such as preeclampsia and intrauterine growth restriction. PMID: 32408878 [PubMed - in process] (Source: Reproductive Biology)
Source: Reproductive Biology - May 14, 2020 Category: Reproduction Medicine Authors: Dickson MA, Peterson N, McRae KE, Pudwell J, Tayade C, Smith GN Tags: Reprod Biol Endocrinol Source Type: research

Association between endometrial thickness and neonatal outcomes in intrauterine insemination cycles: a retrospective analysis of 1,016 live-born singletons.
CONCLUSIONS: EMT is not independently associated with adverse perinatal outcomes in IUI cycles. This novel finding would provide reassuring information for IUI patients with thin endometrial linings regarding their neonatal health. However, further prospective cohort studies with larger datasets are needed to confirm the conclusion. PMID: 32410619 [PubMed - in process] (Source: Reproductive Biology)
Source: Reproductive Biology - May 14, 2020 Category: Reproduction Medicine Authors: Huang J, Lin J, Lu X, Gao H, Song N, Cai R, Kuang Y Tags: Reprod Biol Endocrinol Source Type: research

The relationship between H19 and parameters of ovarian reserve.
Abstract CONTEXT: The H19 long noncoding RNA (lncRNA) belongs to a highly conserved, imprinted gene cluster involved in embryonic development and growth control. We previously described a novel mechanism whereby the Anti-mullerian hormone (Amh) appears to be regulated by H19. However, the relationship between circulating H19 and markers of ovarian reserve including AMH not been investigated. OBJECTIVE: To determine whether H19 expression is altered in women with decreased ovarian reserve. DESIGN: Experimental study. SETTING: Yale School of Medicine (New Haven, USA) and Gazi University School of Medi...
Source: Reproductive Biology - May 13, 2020 Category: Reproduction Medicine Authors: Xia X, Burn MS, Chen Y, Karakaya C, Kallen A Tags: Reprod Biol Endocrinol Source Type: research

COVID-19 and assisted reproductive technology services: repercussions for patients and proposal for individualized clinical management.
oque M, Papanikolaou E, Banker M, Dahan MH, Foong S, Tournaye H, Blockeel C, Vaiarelli A, Humaidan P, Ubaldi FM, POSEIDON (Patient-Oriented Strategies Encompassing IndividualizeD Oocyte Number) group Abstract The prolonged lockdown of health services providing high-complexity fertility treatments -as currently recommended by many reproductive medicine entities- is detrimental for society as a whole, and infertility patients in particular. Globally, approximately 0.3% of all infants born every year are conceived using assisted reproductive technology (ART) treatments. By contrast, the total number of COVID-19 death...
Source: Reproductive Biology - May 13, 2020 Category: Reproduction Medicine Authors: Alviggi C, Esteves SC, Orvieto R, Conforti A, La Marca A, Fischer R, Andersen CY, Bühler K, Sunkara SK, Polyzos NP, Strina I, Carbone L, Bento FC, Galliano D, Yarali H, Vuong LN, Grynberg M, Drakopoulos P, Xavier P, Llacer J, Neuspiller F, Horton M, Roqu Tags: Reprod Biol Endocrinol Source Type: research

Effect of Letrozole on sperm parameters, chromatin status and ROS level in idiopathic Oligo/Astheno/Teratozoospermia.
CONCLUSIONS: Letrozole treatment can effectively increase spontaneous pregnancies by improving sperm parameters and sperm chromatin integrity in men with iOAT and T:E2 ratio ≤ 10. TRIAL REGISTRATION: Trial registration: IRCT, IRCT20191030045283N1. Registered 16 November 2019 - Retrospectively registered, https://fa.irct.ir/user/trial/43484/view. PMID: 32404173 [PubMed - in process] (Source: Reproductive Biology)
Source: Reproductive Biology - May 13, 2020 Category: Reproduction Medicine Authors: Kooshesh L, Bahmanpour S, Zeighami S, Nasr-Esfahani MH Tags: Reprod Biol Endocrinol Source Type: research

Prone positioning and high-flow oxygen improved respiratory function in a 25-week pregnant woman with COVID-19.
PMID: 32405137 [PubMed - as supplied by publisher] (Source: Reproductive Biology)
Source: Reproductive Biology - May 13, 2020 Category: Reproduction Medicine Authors: Vibert F, Kretz M, Thuet V, Barthel F, De Marcillac F, Deruelle P, Lecointre L Tags: Eur J Obstet Gynecol Reprod Biol Source Type: research

Short- and long-term exposure to methamidophos impairs spermatogenesis in mice.
This study aimed to assess the joint action of treatment time and exposure to methamidophos on the male reproductive system. MET was orally administered to adult male Swiss mice at a dose of 0.004 mg.kg-1 for 15 and 50 consecutive days. The following parameters were evaluated: weight of reproductive organs, spermatogenesis, sperm and Sertoli cell count, daily sperm production and sperm transit time. Short-term exposure to methamidophos induced a decrease in epididymal weight. The frequency of stages V-VI of spermatogenesis increased and the frequency of stage IX decreased. In the epididymis, sperm transit time (capu...
Source: Reproductive Biology - May 13, 2020 Category: Reproduction Medicine Authors: Carvalho RK, Rodrigues TC, Júnior WD, Mota GMP, Andersen ML, Mazaro-Costa R Tags: Reprod Biol Source Type: research

Association between the quality of inner cell mass and first trimester miscarriage after single blastocyst transfer.
CONCLUSIONS: Chromosomal aberration of embryo is an important genetic factor for first trimester miscarriage, and the quality of ICM is a potential indicator for euploid miscarriage. Blastocysts with grade A of ICM should be given priority during single blastocyst transfer to reduce potential miscarriage. PMID: 32398002 [PubMed - in process] (Source: Reproductive Biology)
Source: Reproductive Biology - May 12, 2020 Category: Reproduction Medicine Authors: Shi D, Xu J, Zhang M, Niu W, Shi H, Yao G, Li Y, Zhang N, Sun Y Tags: Reprod Biol Endocrinol Source Type: research

Cryopreservation of single-sperm: where are we today?
CONCLUSION: Our review article describes the latest and current technologies implemented for the cryopreservation of single sperm that could potentially benefit patients with severe oligospermia and who rarely have any sperm in their ejaculate. This review provides a platform to understand the process and pitfalls of single-sperm cryopreservation to ensure further improvements in the cryopreservation technology in future studies. PMID: 32398019 [PubMed - in process] (Source: Reproductive Biology)
Source: Reproductive Biology - May 12, 2020 Category: Reproduction Medicine Authors: Liu S, Li F Tags: Reprod Biol Endocrinol Source Type: research

Study on follicular fluid metabolomics components at different ages based on lipid metabolism.
CONCLUSIONS: Metabolomic analysis of follicular fluid revealed that with the increase in age, several differential metabolites are at play. Among these metabolites, lipid metabolism undergoes significant changes that affect the development of oocytes thus causing reduced fertility in older women. These differential metabolites related to follicular development may provide possible detection and treatment targets for promoting oocyte health, and provide scientific basis for understanding the environment of oocyte development. PMID: 32398082 [PubMed - in process] (Source: Reproductive Biology)
Source: Reproductive Biology - May 12, 2020 Category: Reproduction Medicine Authors: Zhang X, Wang T, Song J, Deng J, Sun Z Tags: Reprod Biol Endocrinol Source Type: research

The effect of different estradiol levels on carotid artery distensibility during a long agonist IVF protocol.
This study was made to figure out, does low and high estradiol levels during in vitro fertilization (IVF) cycles have a different effect on carotid artery distensibility (Cdis), carotid artery diameter (Cdia), blood pressure and metabolic factors? Can the stimulation protocol be considered safe to women's vasculature? METHODS: We studied 28 women having a long agonist protocol IVF-treatment in Kuopio University Hospital during the years 2011-2016. Patients were examined at three time points: in the beginning of their own period (low estradiol), during the gonadotrophin releasing hormone (GnRH) analogue downregulation ...
Source: Reproductive Biology - May 12, 2020 Category: Reproduction Medicine Authors: Leppänen J, Randell K, Schwab U, Pihlajamäki J, Keski-Nisula L, Laitinen T, Heinonen S Tags: Reprod Biol Endocrinol Source Type: research

Inhibition of germinal vesicle breakdown using IBMX increases microRNA-21 in the porcine oocyte.
The objective of this study was to characterize the abundance and regulation of MIR21 in relation to GVBD. METHODS: Oocytes were collected from aspirated porcine tertiary follicles. Relative abundance of mature MIR21 was quantified at 0, 8, 16, 24, 32, and 42 h of in vitro (IVM) with or without treatment with 3-isobutyl-1-methylxanthine (IBMX). RESULTS: IBMX increased abundance of MIR21 at 24 h approximately 30-fold compared to control oocytes (P 
Source: Reproductive Biology - May 11, 2020 Category: Reproduction Medicine Authors: Hale BJ, Li Y, Adur MK, Ross JW Tags: Reprod Biol Endocrinol Source Type: research

Prostaglandin E2 affects in vitro maturation of bovine oocytes.
In this study, we supplemented a maturation medium with PGE2 and monitored oocyte quality markers, glucose metabolism, mitochondrial status, oxidative stress, and apoptosis in the cumulus-oocyte complexes (COCs), using a well-established in vitro model of embryo production in cattle. We found that this increased availability of PGE2 during maturation led to an increase in the expression of genes associated with oocyte competence and improved the quality of blastocysts produced. Prostaglandin E2 also appeared to stimulate glucose uptake and lactate production in the COCs, both influencing the expression of enzymes involved ...
Source: Reproductive Biology - May 11, 2020 Category: Reproduction Medicine Authors: Boruszewska D, Kowalczyk-Zieba I, Suwik K, Staszkiewicz-Chodor J, Jaworska J, Lukaszuk K, Woclawek-Potocka I Tags: Reprod Biol Endocrinol Source Type: research

MPA given orally during the first trimester for threatened miscarriage carries no specific risk for foetal abnormalities albeit the rate is higher than non-threatened pregnancies.
Abstract This observational study examines the outcomes of pregnancies arising in women referred for infertility, where those who experienced threatened miscarriage were treated with medroxyprogesterone acetate (MPA) tablets. The 14-year study period covers comprehensive real-time data entries into the validated electronic database including details of the infertility management, pregnancy outcomes and any foetal anomalies among the infants, each being tracked and recorded. Of 4057 clinical pregnancies, 1343 received MPA for threatened miscarriage; 934 (69.6 %) of which continued to livebirths. These were compared...
Source: Reproductive Biology - May 7, 2020 Category: Reproduction Medicine Authors: Yovich JL, Mariappen U, Hinchliffe PM, Dhaliwal SS, Keane KN Tags: Reprod Biol Source Type: research

Impact of human papillomavirus infection in semen on sperm progressive motility in infertile men: a systematic review and meta-analysis.
CONCLUSIONS: HPV semen infection could significantly reduce sperm progressive motility in infertile individuals. There were some limitations in the study such as the differences in age, sample sizes and the number of HPV genotypes detected. Further evidences are needed to better elucidate the relationship between HPV seminal infection and sperm quality. PMID: 32381092 [PubMed - in process] (Source: Reproductive Biology)
Source: Reproductive Biology - May 7, 2020 Category: Reproduction Medicine Authors: Cao X, Wei R, Zhang X, Zhou J, Lou J, Cui Y Tags: Reprod Biol Endocrinol Source Type: research

Molecular analysis of lipid uptake- and necroptosis-associated factor expression in vitrified-warmed mouse oocytes.
CONCLUSIONS: We report that oocytes from aged mice show differential expression of CD36, which suggests that CD36-mediated lipid uptake may be influenced by age. We also show for the first time that pMLKL and pRIPK1 exhibit unique localization pattern in mouse oocytes and this may suggest role(s) for these factors in non-necroptosis-associated cellular processes. PMID: 32366306 [PubMed - in process] (Source: Reproductive Biology)
Source: Reproductive Biology - May 4, 2020 Category: Reproduction Medicine Authors: Um DE, Shin H, Park D, Ahn JM, Kim J, Song H, Lim HJ Tags: Reprod Biol Endocrinol Source Type: research

Increased risk of maternal and neonatal complications in hormone replacement therapy cycles in frozen embryo transfer.
CONCLUSIONS: The HRT protocol for endometrial preparation during frozen embryo transfer of blastocysts was associated with increased risk of maternal and neonatal complications, compared to the NC and OI protocol. PMID: 32366332 [PubMed - in process] (Source: Reproductive Biology)
Source: Reproductive Biology - May 4, 2020 Category: Reproduction Medicine Authors: Zong L, Liu P, Zhou L, Wei D, Ding L, Qin Y Tags: Reprod Biol Endocrinol Source Type: research

Does body mass index impact assisted reproductive technology treatment outcomes in gestational carriers.
CONCLUSION: BMI is not a reliable predictor of outcomes among gestational carriers. PMID: 32359356 [PubMed - in process] (Source: Reproductive Biology)
Source: Reproductive Biology - May 2, 2020 Category: Reproduction Medicine Authors: Fuchs Weizman N, Defer MK, Montbriand J, Pasquale JM, Silver A, Librach CL Tags: Reprod Biol Endocrinol Source Type: research

Clinical, hormonal and genetic characteristics of androgen insensitivity syndrome in 39 Chinese patients.
CONCLUSIONS: Forty-six XY DSD patients with cryptorchidism and female phenotypes were highly suspected of having AIS. We demonstrated that CAIS patients could not be distinguished by their hormone levels alone. Compared with PAIS patients, CAIS patients exhibited higher basal FSH, peak FSH, and peak LH hormone levels but lower AMH expression. We identified 21 reported pathogenic AR mutations and 9 novel AR mutations that led to different types of AIS. Missense mutations were the major cause of AIS and mostly occurred in exon 7 of the AR gene. These findings provided deeper insight into the diagnosis and classification of A...
Source: Reproductive Biology - April 28, 2020 Category: Reproduction Medicine Authors: Liu Q, Yin X, Li P Tags: Reprod Biol Endocrinol Source Type: research

Using ultrasound to define the time point of intrauterine growth retardation in a mouse model of heme oxygenase-1 deficiency.
Abstract The enzyme heme oxygenase-1 (HO-1), encoded by the HMOX1 gene, mediates heme catabolism by cleaving free heme. We have previously revealed the importance of HO-1 in pregnancy. Here, we determined the impact of maternal or paternal HO-1 deficiency on fetal growth and placental parameters throughout gestation. We mated Hmox1-sufficient (WT), partial (HET)- or total (KO)-deficient BALB/c female mice with Hmox1-WT or -KO BALB/c males and performed ultrasound analysis to monitor placental and fetal growth. Doppler measurements were used to determine maternal blood flow parameters. Offspring weights and feto-pl...
Source: Reproductive Biology - April 28, 2020 Category: Reproduction Medicine Authors: Meyer N, Langwisch S, Scharm M, Zenclussen AC Tags: Biol Reprod Source Type: research

Do state insurance mandates alter ICSI utilization?
Abstract BACKGROUND: Assisted reproductive technology (ART) insurance mandates resulted in improved access to infertility treatments like intracytoplasmic sperm injection (ICSI). Our objective was to examine whether ART insurance mandates demonstrate an increased association with ICSI use. METHODS: In this retrospective cohort study, clinic-specific data for 2000-2016 from the Centers for Disease Control (CDC) were grouped by state and subgrouped by the presence and extent of ART state insurance mandates. Mandated (n = 8) and non-mandated (n = 22) states were compared for ICSI use ...
Source: Reproductive Biology - April 25, 2020 Category: Reproduction Medicine Authors: Zagadailov P, Seifer DB, Shan H, Zarek SM, Hsu AL Tags: Reprod Biol Endocrinol Source Type: research

Decreased brain and muscle ARNT-like protein 1 expression mediated the contribution of hyperandrogenism to insulin resistance in polycystic ovary syndrome.
CONCLUSIONS: Decreased BMAL1 expression in the liver and adipose played a potentially novel role in the contribution of hyperandrogenism to insulin resistance, which might be a possible mechanism accounting for the pathogenesis of PCOS. PMID: 32334629 [PubMed - in process] (Source: Reproductive Biology)
Source: Reproductive Biology - April 25, 2020 Category: Reproduction Medicine Authors: Zhai J, Li S, Hu M, Di F, Liu J, Du Y Tags: Reprod Biol Endocrinol Source Type: research

Mouse embryos exposed to oxygen concentrations that mimic changes in the oviduct and uterus show improvement in blastocyst rate, blastocyst size, and accelerated cell division.
This study investigates the effect of a protocol aimed at closely reproducing that natural oxygen concentration in the oviduct (7 % O2 from day 1 to day 3 and 2 % from day 3 to day 5), in contrast to the concentrations (5 % or 20 %) widely used in practice in ART using morphokinetic. Female mice (BI6/CBAca) were sacrificed, and zygotes were isolated 20 h after mating and randomly allocated to three parallel groups, which were grown under high atmospheric, low, or sequential oxygen concentrations. Zygotes were cultured in GTL medium (Vitrolife) and observed by the Primovision time-lapse system. Blastocyst rate at 120 h in t...
Source: Reproductive Biology - April 18, 2020 Category: Reproduction Medicine Authors: Nguyen AQ, Bardua I, Greene B, Wrenzycki C, Wagner U, Ziller V Tags: Reprod Biol Source Type: research

Serine protease inhibitor disrupts sperm motility leading to reduced fertility in female mice.
In this study, we used a mouse model to test the efficacy of AEBSF as a reversible contraceptive, a sperm motility inhibitor, and a spermicide. Additionally, this study evaluated the toxicity of AEBSF on mouse vaginal tissues in vivo and human endocervical cells in vitro. We found that female mice treated with AEBSF had significantly less pups born per litter as well as fertilization rates in vivo compared to the vehicle control. We then showed that AEBSF reduced sperm motility and fertilization capability in vitro in a dose-dependent manner. Furthermore, AEBSF also exhibited spermicidal effects. Lastly, AEBSF treatment in...
Source: Reproductive Biology - April 18, 2020 Category: Reproduction Medicine Authors: Barton BE, Rock JK, Willie AM, Harris EA, Finnerty RM, Herrera GG, Anamthathmakula P, Winuthayanon W Tags: Biol Reprod Source Type: research

Disruption of androgen signaling during puberty affects Notch pathway in rat seminiferous epithelium.
Abstract BACKGROUND: Onset of spermatogenesis at puberty is critically dependent on the activity of hypothalamic-pituitary-gonadal axis and testosterone production by Leydig cells. The aim of this study was to examine whether activation of Notch receptors and expression of Notch ligands and effector genes in rat seminiferous epithelium are controlled by androgen signaling during puberty. METHODS: Peripubertal (5-week-old) Wistar rats received injections of flutamide (50 mg/kg bw) daily for 7 days to reduce androgen receptor (AR) signaling or a single injection of ethanedimethane sulphonate (EDS; 75&thi...
Source: Reproductive Biology - April 16, 2020 Category: Reproduction Medicine Authors: Kamińska A, Marek S, Pardyak L, Brzoskwinia M, Pawlicki P, Bilińska B, Hejmej A Tags: Reprod Biol Endocrinol Source Type: research

hsa-miR-100-5p, an overexpressed miRNA in human ovarian endometriotic stromal cells, promotes invasion through attenuation of SMARCD1 expression.
CONCLUSION: We found that SMARCD1/MMP-1 is a downstream pathway of hsa-miR-100-5p. hsa-miR-100-5p transfection enhanced the motility of NESCs by inhibiting SMARCD1 expression and MMP1 activation. These findings suggest that enhanced hsa-miR-100-5p expression in endometriosis is involved in promoting the acquisition of endometriosis-specific characteristics during endometriosis development. Our present findings on the roles of hsa-miR-100-5p may thus contribute to understand the epigenetic mechanisms involved in the pathogenesis of endometriosis. PMID: 32299427 [PubMed - in process] (Source: Reproductive Biology)
Source: Reproductive Biology - April 16, 2020 Category: Reproduction Medicine Authors: Takebayashi K, Nasu K, Okamoto M, Aoyagi Y, Hirakawa T, Narahara H Tags: Reprod Biol Endocrinol Source Type: research

A prospective, single-centre, single-arm, open label study of the long term use of a gonadotropin releasing hormone agonist (Triptorelin SR, 11.25  mg) in combination with Tibolone add-back therapy in the management of chronic cyclical pelvic pain.
CONCLUSION: Long- term Triptorelin plus Tibolone add-back therapy in women suffering from CCPP does not appear to be associated with significant serious adverse events apart from the possibility of deterioration in the BMD that needs to be monitored. This mode of therapy appears to be effective in pain relief and in improving quality of life over a 24-month period. TRIAL REGISTRATION: Clinical trials database NCT00735852. PMID: 32290838 [PubMed - in process] (Source: Reproductive Biology)
Source: Reproductive Biology - April 14, 2020 Category: Reproduction Medicine Authors: Alshehre SM, Duffy S, Jones G, Ledger WL, Metwally M Tags: Reprod Biol Endocrinol Source Type: research

Performing ICSI within 4 hours after denudation optimizes clinical outcomes in ICSI cycles.
Abstract BACKGROUND: The study aimed to investigate whether and how general and partial time intervals between processes, from human chorionic gonadotrophin (HCG) trigger to intracytoplasmic sperm injection (ICSI), affected the laboratory and reproductive outcomes in ICSI cycles. METHODS: This was a retrospective data analysis of 3602 women who underwent ICSI treatment cycles using partner or donor sperms, performed at Reproduction Medicine Center of Tongji Hospital of Tongji Medical College of Huazhong University of Science and Technology (Wuhan, China) between October 2016 and September 2018. The clinical p...
Source: Reproductive Biology - April 14, 2020 Category: Reproduction Medicine Authors: Zhang Y, Ma Y, Fang Z, Hu S, Li Z, Zhu L, Jin L Tags: Reprod Biol Endocrinol Source Type: research

Effect of cold stress on ovarian & uterine microcirculation in rats and the role of endothelin system.
CONCLUSIONS: This study suggests that the reason for cold stress-induced dysfunction in reproductive organs may be closely related to the imbalance of ET-1 and its receptor expressions, leading to microvascular circulation disorders in local tissues. PMID: 32290862 [PubMed - in process] (Source: Reproductive Biology)
Source: Reproductive Biology - April 14, 2020 Category: Reproduction Medicine Authors: Wang D, Cheng X, Fang H, Ren Y, Li X, Ren W, Xue B, Yang C Tags: Reprod Biol Endocrinol Source Type: research

A novel approach for human sperm cryopreservation with AFPIII.
In conclusion, the results showed that the addition of AFPIII to GEYC at 1 μg/ml improved motility, PMI, viability and TAC, and decreased ROS and DNA fragmentation of cryopreserved human semen compared to the control group. PMID: 32279880 [PubMed - as supplied by publisher] (Source: Reproductive Biology)
Source: Reproductive Biology - April 10, 2020 Category: Reproduction Medicine Authors: Zandiyeh S, Shahverdi A, Ebrahimi B, Sabbaghian M Tags: Reprod Biol Source Type: research

Flaws (and quality) in research today: can artificial intelligence intervene?
Abstract The existing flaws in both conducting and reporting of research have been outlined and criticized in the past. Weak research design, poor methodology, lack of fresh ideas and poor reporting are the main points to blame. Issues have been continually raised on the types of results published, review process, sponsorship, notion, ethics, and incentives in publishing, the role of regulatory agencies and stakeholders, the role of funding, and the cooperation between funders and academic institutions and the training of both clinicians and methodologists or statisticians. As a result, there is loss of the utmost...
Source: Reproductive Biology - April 8, 2020 Category: Reproduction Medicine Authors: Siristatidis C, Pouliakis A Tags: Syst Biol Reprod Med Source Type: research

MiR-423-5p may regulate ovarian response to ovulation induction via CSF1.
The objective of the present study was to find the target gene and mechanism for miR-423-5p involved in ovarian response regulation. METHODS: (a) TargetScan was used to predict the target gene of hsa-miR-423-5p. (b) A model for hsa-miR-423-5p overexpression or inhibition was constructed by transfecting KGN cells with lentivirus. CSF1 mRNA and protein expression and luciferase activity were measured. (c) The cell cycles of control and lentivirus treated KGN cells were analyzed. Western blot was used to measure the expression of CDKN1A in KGN cells. (d) The concentration of E2 in KGN cell culture medium were measured. ...
Source: Reproductive Biology - April 7, 2020 Category: Reproduction Medicine Authors: Xie S, Zhang Q, Zhao J, Hao J, Fu J, Li Y Tags: Reprod Biol Endocrinol Source Type: research

Chronic unpredictable stress-induced reproductive deficits were prevented by probiotics.
Abstract Stress can induce reproductive deficits by activating the HPA and causing oxidative stress. Some studies have indicated that the neurologic diseases or disorders induced by stress could be relieved by probiotics. Whether chronic unpredictable stress (CUS)-induced reproductive deficits could be prevented by probiotics is unclear. The present experiment was designed to evaluate the effects of L. rhamnosus Gorbach-Goldin (LGG) on CUS-induced reproductive deficits. Kunming mice were divided into control, stress, and LGG groups randomly. The mice in stress and LGG groups were exposed to CUS for 40days, in the ...
Source: Reproductive Biology - April 5, 2020 Category: Reproduction Medicine Authors: Guo Y, Du X, Bian Y, Wang S Tags: Reprod Biol Source Type: research

Leptin-induced increase in blood pressure and markers of endothelial activation during pregnancy in Sprague Dawley rats is prevented by resibufogenin, a marinobufagenin antagonist.
Abstract Levels of leptin and marinobufagenin (MBG), a cardiotonic steroid, are elevated in the serum of women with pre-eclampsia. Besides this, leptin administration to pregnant rats increases systolic blood pressure (SBP), urinary protein excretion and serum markers of endothelial activation. The link between leptin and MBG is unknown and it is also unclear if leptin-induced increases in blood pressure and proteinuria in the pregnant rat could be prevented by an MBG antagonist. To ascertain this link, this study investigated the effect of resibufogenin (RBG), a marinobufagenin antagonist, on leptin-induced incre...
Source: Reproductive Biology - April 3, 2020 Category: Reproduction Medicine Authors: Hassan MJM, Bakar NS, Aziz MA, Basah NK, Singh HJ Tags: Reprod Biol Source Type: research

Expression and localization of Septin 14 gene and protein in infertile men testis.
The objective of this study was to evaluate the gene and protein expression pattern of Septin 14 in the testis tissue of azoospermic men. In addition, Septin 14 localization was also assessed in the sperm. Testicular tissues were obtained from biopsies of non-obstrutive azoospermic men who underwent diagnostic testicular biopsy in Royan institute and were divided into two groups: TESE + with positive result in testicular sperm extraction (with hypospermatogenesis pathology) and TESE- with negative result (included patients with Sertoli cell only syndrome and maturation arrest pathologies). Total RNA and protein was extract...
Source: Reproductive Biology - April 3, 2020 Category: Reproduction Medicine Authors: Vahabi Barzi N, Kakavand K, Sodeifi N, Ghezelayagh Z, Sabbaghian M Tags: Reprod Biol Source Type: research

Zinc deficient diet exacerbates the testicular and epididymal damage in type 2 diabetic rat: Studies on oxidative stress-related mechanisms.
Abstract Zinc (Zn) is one of the most important trace elements in the body and is required for insulin secretion and release. Zn is also required for the growth and development of the reproductive system. Alteration in the Zn levels can cause moderate to severe damage to various organs, including the reproductive system. Most of type 2 diabetic patients have altered Zn levels/signaling. So, here we investigated the role of Zn-deficient diet (ZDD) in type 2 diabetes. Type 2 diabetes in the rat was induced by the combination of high-fat diet (HFD) and a single low dose of streptozotocin (STZ, 35 mg/kg, i.p.). Contro...
Source: Reproductive Biology - March 31, 2020 Category: Reproduction Medicine Authors: Maremanda KP, Srivalliputturu SB, Jena G Tags: Reprod Biol Source Type: research

Italian validation of the German Pelvic Floor Questionnaire for pregnant and postpartum women.
CONCLUSIONS: The Italian version of the German Pelvic Floor Questionnaire for pregnant and postpartum women is reliable, valid and consistent. PMID: 32203824 [PubMed - as supplied by publisher] (Source: Reproductive Biology)
Source: Reproductive Biology - March 19, 2020 Category: Reproduction Medicine Authors: Palmieri S, Cola A, Ceccherelli A, Manodoro S, Frigerio M, Vergani P Tags: Eur J Obstet Gynecol Reprod Biol Source Type: research

Management of reduced fetal movement: A comparative analysis of two audits at a tertiary care clinical service.
CONCLUSION: After implementing the new guideline, the re-audit demonstrates a reduction in the number of requested ultrasound scans without any compromise on the perinatal outcome. PMID: 32203823 [PubMed - as supplied by publisher] (Source: Reproductive Biology)
Source: Reproductive Biology - March 18, 2020 Category: Reproduction Medicine Authors: Kapaya H, Almeida J, Karouni F, Anumba D Tags: Eur J Obstet Gynecol Reprod Biol Source Type: research

Optimism during pregnancy and obstetrical outcomes: A systematic review.
Abstract OBJECTIVE: To ascertain the strength of association between dispositional optimism, assessed with the Revised Life Orientation Test (LOT-R), and obstetrical outcomes, and to evaluate women's social characteristics that may lead to low dispositional optimism during pregnancy. STUDY DESIGN: The research was conducted using MEDLINE, EMBASE, Scopus, Web of Sciences, Cochrane Database, and ClinicalTrial.gov as electronic databases. The articles were identified with the use of a combination of the relevant heading term, key words, and word variants for: "optimism" or "happiness" and &qu...
Source: Reproductive Biology - March 18, 2020 Category: Reproduction Medicine Authors: Giangiordano I, Sahani H, Di Mascio D, Saccone G, Bellussi F, Berghella A, Braverman A, Berghella V Tags: Eur J Obstet Gynecol Reprod Biol Source Type: research