Histologic analysis and lipid profiling reveal reproductive age-associated changes in peri-ovarian adipose tissue.
CONCLUSIONS: These findings pinpoint age-associated physiological changes in peri-gonadal adipose tissue with respect to adipocyte morphology and lipid profiles and lay the foundation for future studies to examine how these alterations may influence both adipocyte and ovarian function. PMID: 31189477 [PubMed - in process] (Source: Reproductive Biology)
Source: Reproductive Biology - June 12, 2019 Category: Reproduction Medicine Authors: Dipali SS, Ferreira CR, Zhou LT, Pritchard MT, Duncan FE Tags: Reprod Biol Endocrinol Source Type: research

Identification of potential metabolic biomarkers of polycystic ovary syndrome in follicular fluid by SWATH mass spectrometry.
Abstract BACKGROUND: Polycystic ovary syndrome (PCOS) is a complex disorder associated with multiple metabolic disturbance, including defective glucose metabolism and insulin resistance. The altered metabolites caused by the related metabolic disturbance may affect ovarian follicles, which can be reflected in follicular fluid composition. The aim of this study is to investigate follicular fluid metabolic profiles in women with PCOS using an advanced sequential window acquisition of all theoretical fragment-ion spectra (SWATH) mass spectrometry. MATERIALS AND METHODS: Nineteen women with PCOS and twenty-one he...
Source: Reproductive Biology - June 11, 2019 Category: Reproduction Medicine Authors: Sun Z, Chang HM, Wang A, Song J, Zhang X, Guo J, Leung PCK, Lian F Tags: Reprod Biol Endocrinol Source Type: research

The biochemistry surrounding bovine conceptus elongation.
gan P Abstract Conceptus elongation is a fundamental developmental event coinciding with a period of significant pregnancy loss in cattle. The process has yet to be recapitulated in vitro whereas in vivo it is directly driven by uterine secretions and indirectly influenced by systemic progesterone. To better understand the environment facilitating this critical reproductive phenomenon, we interrogated the biochemical composition of uterine luminal fluid from heifers with high vs. physiological circulating progesterone on Days 12-14 of the estrous cycle-the window of conceptus elongation-initiation-by high-throughp...
Source: Reproductive Biology - June 10, 2019 Category: Reproduction Medicine Authors: Simintiras CA, Sánchez JM, McDonald M, Lonergan P Tags: Biol Reprod Source Type: research

Up-regulation of AREG, EGFR and HER2 contributes to increased VEGF expression in granulosa cells of patients with OHSS †.
This study demonstrated that granulosa cell-secreted AREG plays an important role in the development of OHSS, suggesting that the EGFR/HER2-mediated signaling could be a novel drug target for the prevention and treatment of OHSS. PMID: 31167229 [PubMed - as supplied by publisher] (Source: Reproductive Biology)
Source: Reproductive Biology - June 5, 2019 Category: Reproduction Medicine Authors: Fang L, Yu Y, Li Y, Wang S, He J, Zhang R, Sun YP Tags: Biol Reprod Source Type: research

Role of activin, follistatin, and inhibin in the regulation of kiss-1 gene expression in hypothalamic cell models.
Abstract Kisspeptin (encoded by the Kiss-1 gene) in the arcuate nucleus (ARC) of the hypothalamus governs the hypothalamic-pituitary-gonadal (HPG) axis by regulating pulsatile release of gonadotropin-releasing hormone (GnRH). Meanwhile, kisspeptin in the anteroventral periventricular nucleus (AVPV) region has been implicated in estradiol (E2)-induced GnRH surges. Kiss-1-expressing cell model mHypoA-55 exhibits characteristics of Kiss-1 neurons in the ARC region. On the other hand, Kiss-1 expressing mHypoA-50 cells originate from the AVPV region. In the mHypoA-55 ARC cells, activin significantly increased Kiss-1 ge...
Source: Reproductive Biology - June 5, 2019 Category: Reproduction Medicine Authors: Tumurgan Z, Kanasaki H, Tumurbaatar T, Oride A, Okada H, Hara T, Kyo S Tags: Biol Reprod Source Type: research

Abuse in assisted reproductive technology: A systematic qualitative review and typology.
CONCLUSIONS: A wide range of categories of abuse was found despite the paucity of formal literature. A concerted public health approach to infertility is required, combined with an emphasis on trying conservative approaches first. More primary research is required on prevalence of abuse, and values and preferences, particularly in "egg sharing" and post-mortem reproduction. PMID: 31160133 [PubMed - as supplied by publisher] (Source: Reproductive Biology)
Source: Reproductive Biology - May 29, 2019 Category: Reproduction Medicine Authors: Hodson N, Bewley S Tags: Eur J Obstet Gynecol Reprod Biol Source Type: research

Predictors of patient preference for mode of delivery following an obstetric anal sphincter injury.
Abstract OBJECTIVES: Patients who sustain an Obstetric Anal Sphincter Injury (OASI) have the opportunity to select an elective caesarean section over a vaginal delivery in subsequent pregnancies. It remains unclear whether there are identifiable factors which predict expectant mothers' choices. The primary aim of our study was to explore this issue further in a consecutive group of patients who had suffered OASI. STUDY DESIGN: Data were retrospectively collected for patients attending a specialist OASIS clinic between July 2016 and February 2018. Information routinely collected in clinical practice was consid...
Source: Reproductive Biology - May 29, 2019 Category: Reproduction Medicine Authors: Cole J, Bulchandani S Tags: Eur J Obstet Gynecol Reprod Biol Source Type: research

Nocturnal blood pressure alterations in patients with preeclampsia - Do they really matter? A systematic review of the literature.
Abstract The significance of blood pressure alterations during night-time has been already recorded in essential hypertension and several studies have been conducted to guide current clinical practice. To date, however, there is no consensus regarding the need for screening patients with preeclampsia for nocturnal hypertension as evidence in this field remain scarce. The purpose of this study is to accumulate current data in this field and serve as a pilot for the conduct of future studies. The present systematic review was designed according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyse...
Source: Reproductive Biology - May 29, 2019 Category: Reproduction Medicine Authors: Pergialiotis V, Karampetsou N, Bellos I, Papantoniou N, Daskalakis G Tags: Eur J Obstet Gynecol Reprod Biol Source Type: research

Mice lacking membrane estrogen receptor 1 are protected from reproductive pathologies resulting from developmental estrogen exposure.
Abstract Both membrane and nuclear fractions of estrogen receptor 1 (ESR1) mediate 17β-estradiol (E2) actions. Mice expressing nuclear (n)ESR1 but lacking membrane (m)ESR1 (nuclear-only estrogen receptor 1 [NOER] mice) show reduced E2 responsivity and reproductive abnormalities culminating in adult male and female infertility. Using this model, we investigated whether reproductive pathologies caused by the synthetic estrogen diethylstilbestrol (DES) are mitigated by mESR1 ablation. Homozygous and heterozygous wild-type (WT and HET, respectively) and NOER male and female mice were subcutaneously injected with ...
Source: Reproductive Biology - May 29, 2019 Category: Reproduction Medicine Authors: Nanjappa MK, Medrano TI, Mesa AM, Ortega MT, Caldo PD, Mao J, Kinkade JA, Levin ER, Rosenfeld CS, Cooke PS Tags: Biol Reprod Source Type: research

Early onset of cabergoline therapy for prophylaxis from ovarian hyperstimulation syndrome (OHSS): A potentially safer and more effective protocol.
This study aimed at evaluating the effectiveness of early administration of cabergoline in the prevention of OHSS in high risk cases prepared for ICSI. This case series study was conducted on 126 high risk patients prepared for ICSI using the fixed antagonist protocol. High risk patients were defined as having more than 20 follicles>12 mm in diameter, and/or E2 more than 3000 pg/ml when the size of the leading follicle is more than 15 mm. When the size of the leading follicle reached 15 mm, cabergoline was administered (0.5 mg/day) for 8 days. Patients were followed up clinically, ultrasonographically and hema...
Source: Reproductive Biology - May 25, 2019 Category: Reproduction Medicine Authors: Gaafar S, Mahdavi M Tags: Reprod Biol Source Type: research

Cystic hygroma and micromelic lower limbs: First-trimester sonographic markers of campomelic dysplasia.
PMID: 31133349 [PubMed - as supplied by publisher] (Source: Reproductive Biology)
Source: Reproductive Biology - May 21, 2019 Category: Reproduction Medicine Authors: Zhen L, Xu LL, Li DZ Tags: Eur J Obstet Gynecol Reprod Biol Source Type: research

Cesarean scar pregnancy managed with local and systemic methotrexate: A single center case series.
CONCLUSIONS: Treatment of cesarean scar pregnancy with a combination of systemic and local methotrexate was effective and safe. Although the treatment course tends to be longer than with other modalities, this protocol offers excellent success rates, with fertility preservation and few complications. PMID: 31132691 [PubMed - as supplied by publisher] (Source: Reproductive Biology)
Source: Reproductive Biology - May 20, 2019 Category: Reproduction Medicine Authors: Naeh A, Shrim A, Shalom-Paz E, Amir M, Hallak M, Bruchim I Tags: Eur J Obstet Gynecol Reprod Biol Source Type: research

Development and initial validation of an electronic personal assessment questionnaire for menstrual, pelvic pain and gynaecological hormonal disorders (ePAQ-MPH).
Abstract OBJECTIVE: Menstrual disorders, pelvic-pain and gynaecological hormonal conditions in women can have a significant impact on quality-of-life. Reliable assessment and monitoring of these intimate conditions is challenging. Patient reported outcome measures (PROMs) can be invaluable in providing objective assessment, but no comprehensive PROM assessing all of these conditions and their impact on quality of life is currently available. The purpose of this study was to develop and undertake initial psychometric testing of a comprehensive interactive electronic patient reported outcome measure for these condit...
Source: Reproductive Biology - May 20, 2019 Category: Reproduction Medicine Authors: Gray TG, Moores KL, James E, Connor ME, Jones GL, Radley SC Tags: Eur J Obstet Gynecol Reprod Biol Source Type: research

Incidence and recurrence rate of postpartum hemorrhage and manual removal of the placenta: A longitudinal linked national cohort study in The Netherlands.
CONCLUSION: Women with a previous delivery complicated by postpartum hemorrhage or manual removal of the placenta are at increased risk for recurrence. The magnitude of this risk is highest in women with deliveries beyond 32 weeks in the first pregnancy. PMID: 31129559 [PubMed - as supplied by publisher] (Source: Reproductive Biology)
Source: Reproductive Biology - May 19, 2019 Category: Reproduction Medicine Authors: Ruiter L, Kazemier BM, Mol BWJ, Pajkrt E Tags: Eur J Obstet Gynecol Reprod Biol Source Type: research

Is scanning for vasa previa important for singleton pregnancies that started as multiple conceptions?
Abstract OBJECTIVES: Vasa previa (VP) is a congenital placentation disorder in which fetal vessels run across the internal os of the cervix under the fetal presentation. This rare condition is associated with a high rate of perinatal morbidity and mortality when undetected before delivery. Roughly 85% of all cases of VP can be associated with one or more identifiable risk factors including in-vitro fertilization (IVF), multiple gestations, bilobed, succenturiate or low-lying placentas, and velamentous cord insertion (VCI). Recent evidence indicates the need for standardized prenatal targeted scanning protocols of ...
Source: Reproductive Biology - May 18, 2019 Category: Reproduction Medicine Authors: Melcer Y, Pekar-Zlotin M, Wolf B, Betser M, Dvash S, Zilberman Sharon N, Maymon R Tags: Eur J Obstet Gynecol Reprod Biol Source Type: research

Morphokinetic characteristics of embryos originating from extremely small follicles: A prospective study.
Abstract OBJECTIVE: To investigate the developmental potential of oocytes and embryos derived from extremely small follicles (
Source: Reproductive Biology - May 18, 2019 Category: Reproduction Medicine Authors: Avraham S, Kalma Y, Azem F, Zakar L, Amir H, Rahav R, Barzilay L, Dviri M, Almog B Tags: Eur J Obstet Gynecol Reprod Biol Source Type: research

Reproductive complications in celiac disease patients in Slovenia.
urk D Abstract OBJECTIVE: Celiac disease is associated with higher risk of infertility, recurrent abortions, and adverse outcomes in pregnancy and in puerperium. The aim of the study was to analyse the association between celiac disease and reproductive disorders in the group of celiac patients and compare these to healthy controls. METHODS: A retrospective case-control matched study. The association between celiac disease and menstrual cycle, gyneco-obstetrical complications was assessed with a questionnaire specifically developed for the study. 144 celiac women and 61 celiac men, members of Slovenian Celiac...
Source: Reproductive Biology - May 15, 2019 Category: Reproduction Medicine Authors: Pogačar MŠ, Vlaisavljević V, Turk E, Mičetić-Turk D Tags: Eur J Obstet Gynecol Reprod Biol Source Type: research

Increased placental expressions of nuclear factor erythroid 2-related factor 2 and antioxidant enzymes in gestational diabetes: Protective mechanisms against the placental oxidative stress?
CONCLUSION: Maternal, fetal and placental oxidative stress was observed in gestational diabetes mellitus. The gestational diabetic placenta had an increased Nrf2 protein expression. The activated placental Nrf2/ antioxidant response element (ARE) pathway might have led to an increased expression of antioxidant enzymes SOD1 and catalase. This may be viewed as a protective mechanism in placenta from the further onslaught of oxidative stress. PMID: 31121342 [PubMed - as supplied by publisher] (Source: Reproductive Biology)
Source: Reproductive Biology - May 15, 2019 Category: Reproduction Medicine Authors: Manoharan B, Bobby Z, Dorairajan G, Jacob SE, Gladwin V, Vinayagam V, Packirisamy RM Tags: Eur J Obstet Gynecol Reprod Biol Source Type: research

Blastomere movement post first cell division correlates with embryonic compaction and subsequent blastocyst formation.
CONCLUSIONS: Embryonic compaction and subsequent blastocyst formation are adversely affected by twist-and-crumble movement and prolonged movement after the first cell division. Our results indicate that the preimplantation developmental competence of human embryos could be predicted by assessing BMov after the first cell division on day 1. PMID: 31092247 [PubMed - in process] (Source: Reproductive Biology)
Source: Reproductive Biology - May 15, 2019 Category: Reproduction Medicine Authors: Ohata K, Ezoe K, Miki T, Morita H, Tsuchiya R, Kaneko S, Okimura T, Uchiyama K, Yabuuchi A, Kobayashi T, Montag M, Kato K Tags: Reprod Biol Endocrinol Source Type: research

Ice age: Cryopreservation in assisted reproduction - An update.
cio A Abstract Since the first reported birth following in vitro fertilization in 1978, further developments in assisted reproductive technology (ART) treatments have produced at least 8 million babies worldwide. Cryopreservation techniques have been central to this treatment revolution, increasing cycle efficacy by allowing the banking of supernumerary embryos for later use, as well as affording the prospective patient more time in cases of anticipated fertility decline. Additionally, these techniques have demonstrated promise in increasing the safety of ART treatments, by reducing complications such as ovarian h...
Source: Reproductive Biology - May 11, 2019 Category: Reproduction Medicine Authors: Rodriguez-Wallberg KA, Waterstone M, Anastácio A Tags: Reprod Biol Source Type: research

Letrozole supplementation during controlled ovarian stimulation in expected high responders: a pilot randomized controlled study.
CONCLUSIONS: In this pilot study, letrozole supplementation could not reduce the incidence of premature progesterone rise during the late follicular phase in stimulated in vitro fertilization cycles in expected high responders, producing a harmful effect on the pregnancy outcome. TRIAL REGISTRATION: China Clinical Trial Registration Center: ChiCTR-IPR-15006211 URL of the trial registry record: http://www.chictr.org.cn/showproj.aspx?proj=10731 . Trial registration date: 8 April, 2015. Date of first patient's enrolment: 5 October, 2015. PMID: 31077214 [PubMed - in process] (Source: Reproductive Biology)
Source: Reproductive Biology - May 10, 2019 Category: Reproduction Medicine Authors: Yang X, Lin G, Lu G, Gong F Tags: Reprod Biol Endocrinol Source Type: research

Effects of resveratrol on reducing spermatogenic dysfunction caused by high-intensity exercise.
Abstract BACKGROUND: Long-term high-intensity exercise can lead to reproductive endocrine and spermatogenic dysfunction. This research is to investigate the effect of resveratrol on the reduction of reproductive dysfunction induced by high-intensity exercise, and to screen relevant factors and signal transduction pathways. METHODS: Rats were randomly divided into three groups, a control group, an intensive exercise group (IE group), and a resveratrol-treated group (RSV group). After 9 weeks of exercise, the sperm density and reproductive hormone concentrations were measured, along with antioxidation, i...
Source: Reproductive Biology - May 6, 2019 Category: Reproduction Medicine Authors: Guo Y, Wang A, Liu X, Li E Tags: Reprod Biol Endocrinol Source Type: research

Analyses of bovine luteal fractions obtained by FACS reveals enrichment of miR-183-96-182 cluster miRNAs in endothelial cells.
Abstract Our previous studies showed that the miRNA clusters, miR-183-96-182 and miR-212-132, may be critical in promoting luteal cell survival and progesterone production in both bovine and humans. To further understand their involvement in luteal development, this study aimed to establish the expression of these miRNAs in different bovine luteal cell types, namely, endothelial and steroidogenic, isolated using fluorescence-activated cell sorting (FACS). We isolated each of the two cell populations based on the presence of the endothelia surface marker, CD144, and uptake of the lipophilic dye, Nile Red, respectiv...
Source: Reproductive Biology - May 6, 2019 Category: Reproduction Medicine Authors: Mohammed BT, Esteves CL, Donadeu FX Tags: Reprod Biol Endocrinol Source Type: research

Intercellular wireless communication network between mother and fetus in rat pregnancy-a study on directed and weighted network.
CONCLUSION: Detection of concentration changes of the factors in maternal serum could provide a tool for monitoring, diagnosis, prediction and treatment of embryo differentiation, development and health. PMID: 31043168 [PubMed - in process] (Source: Reproductive Biology)
Source: Reproductive Biology - May 1, 2019 Category: Reproduction Medicine Authors: Zhao M, Liu T, Pang G Tags: Reprod Biol Endocrinol Source Type: research

G-CSF-mobilized Peripheral Blood Mononuclear Cells Combined with Platelet-rich Plasma Accelerates Restoration of Ovarian Function in Cyclophosphamide-induced POI Rats.
Abstract Peripheral blood mononuclear cells (PBMCs) are rich in hematopoietic cells and mesenchymal stem cells; Platelet-rich plasma (PRP) is rich in various growth factors. PBMCs and PRP have been suggested, individually, to restore ovarian function by improving the local microenvironment. The current study investigated the effect of granulocyte colony-stimulating factor (G-CSF)-mobilized PBMCs combined with PRP on restoring ovarian function in rats with primary ovarian insufficiency (POI). Thirty adult female rats were randomly subdivided into five groups: normal control (control), cyclophosphamide (CTX) plus su...
Source: Reproductive Biology - April 29, 2019 Category: Reproduction Medicine Authors: Huang Q, Liu B, Jiang R, Liao S, Wei Z, Bi Y, Liu X, Deng R, Jin Y, Tan Y, Yang Y, Qin A Tags: Biol Reprod Source Type: research

Possible role of androgen receptor gene in therapeutic response of infertile men with hypogonadotropic hypogonadism.
Abstract Hypogonadotropic hypogonadism (HH) is defined as a dysfunction of hypothalamic-pituitary-gonadal axis, which causes impairments in gametogenesis, pubertal maturation, and/or secretion of the gonadal sex hormones. Human chronic gonadotropin (hCG) stimulates the Leydig cells of the testis to secrete testosterone, which is essential for spermatogenesis. Testosterone replacement therapy is one of the possible options to manage HH treatment. Given the fact that testosterone functions are mediated via androgen receptor (AR), the aim of the present study was to evaluate whether the CAG/GGN triple repeat expansio...
Source: Reproductive Biology - April 28, 2019 Category: Reproduction Medicine Authors: Firouzi V, Borjian Boroujeni P, Rokhsat Talab Z, Mohammadi M, Sadighi Gilani MA, Sabbaghian M, Mohseni Meybodi A Tags: Syst Biol Reprod Med Source Type: research

Microorganisms in the healthy upper reproductive tract: from denial to beneficial assignments for reproductive biology.
Abstract Contrary to the traditional assumption of a sterile uterus, the number of studies characterizing microbial entities in the healthy upper reproductive tract (endometrial cavity, including follicular fluid and placenta) have been on the increase. Substantial data has been accumulated correlating microbial composition with fertility outcome. In this context, the presence of certain taxa was associated to an improved reproductive success. A summarization for the evidence of these molecular mechanisms through which bacteria may affect developmental processes during pregnancy is presented and discussed with spe...
Source: Reproductive Biology - April 22, 2019 Category: Reproduction Medicine Authors: Einenkel R, Zygmunt M, Muzzio DO Tags: Reprod Biol Source Type: research

Improvement of embryonic development and clinical outcomes of germinal vesicle stage oocytes using a microvibration culture system.
This study's objective was to evaluate the effect of a microvibration culture system (MVC) during IVM and/or in vitro culture (IVC) on the clinical outcomes and the embryonic development potential of human GV-stage oocytes collected from human chorionic gonadotropin (HCG)-primed IVM and fertilization-embryo transfer (IVM/F-ET) cycles of patients with polycystic ovaries (PCO). A total of 206 HCG-primed IVM/F-ET cycles were divided into four groups according to the microvibration and static culture system applied during IVM and/or IVC: Group SS (static system during both IVM and IVC); Group SV (static system during IVM alter...
Source: Reproductive Biology - April 19, 2019 Category: Reproduction Medicine Authors: Yang SH, Yoon SH, Jung JH, Lim JH, Ko Y Tags: Syst Biol Reprod Med Source Type: research

Effects of high-fat diets on fetal growth in rodents: a systematic review.
CONCLUSIONS: The high-fat diets used most often with rodents do not closely match Western diets and frequently reduce fetal growth, which is not a typical feature of obese human pregnancies. Adoption of standard protocols that more accurately mimic effects on fetal growth observed in obese human pregnancies will improve translational impact in this field. PMID: 30992002 [PubMed - in process] (Source: Reproductive Biology)
Source: Reproductive Biology - April 16, 2019 Category: Reproduction Medicine Authors: Christians JK, Lennie KI, Wild LK, Garcha R Tags: Reprod Biol Endocrinol Source Type: research

Adipocyte alterations in endometriosis: reduced numbers of stem cells and microRNA induced alterations in adipocyte metabolic gene expression.
CONCLUSIONS: We demonstrate that microRNAs Let-7b and miR-342-3p affected metabolic gene expression significantly in adipocytes of women with endometriosis. Similarly, there is a reduction in the adipose stem cell population in a mouse model of endometriosis. Taken together these data suggest that endometriosis alters BMI in part through an effect on adipocytes and fat metabolism. PMID: 30982470 [PubMed - in process] (Source: Reproductive Biology)
Source: Reproductive Biology - April 15, 2019 Category: Reproduction Medicine Authors: Zolbin MM, Mamillapalli R, Nematian SE, Goetz L, Taylor HS Tags: Reprod Biol Endocrinol Source Type: research

Doxycycline reduces MMP-2 activity and inhibits invasion of 12Z epithelial endometriotic cells as well as MMP-2 and -9 activity in primary endometriotic stromal cells in vitro.
CONCLUSIONS: In conclusion, the MMP-inhibiting features of subantimicrobial-dose doxycycline may be further evaluated as a well-tolerable additional therapeutic approach, e.g. in combination with progestins such as dienogest, in patients with infiltrative endometriosis with insufficient response to current medical treatment options. PMID: 30981279 [PubMed - in process] (Source: Reproductive Biology)
Source: Reproductive Biology - April 13, 2019 Category: Reproduction Medicine Authors: Samartzis EP, Fink D, Stucki M, Imesch P Tags: Reprod Biol Endocrinol Source Type: research

Higher melatonin in the follicle fluid and MT2 expression in the granulosa cells contribute to the OHSS occurrence.
This study aims to identify the predictive value of follicular fluid (FF) melatonin for OHSS establishment and the underlying mechanism. METHODS: All participants of this case-control study were enrolled at the Reproductive Medicine Center located in the First Affiliated Hospital of Zhengzhou University in China from January to October in 2017. Quantitative real-time PCR and western blot were used to examine the mRNA and protein levels. Primary granulosa cells were extracted and cultured for in vitro studies. Melatonin concentration was measured by ELISA. Logistic analysis and receiver-operating characteristic (ROC) c...
Source: Reproductive Biology - April 12, 2019 Category: Reproduction Medicine Authors: Li Y, Fang L, Yu Y, Shi H, Wang S, Guo Y, Sun Y Tags: Reprod Biol Endocrinol Source Type: research

Evaluation of the antioxidative enzymes in the seminal plasma of infertile men: Contribution to classic semen quality analysis.
Abstract Protein expression/activity of antioxidative defense enzymes (AD) in seminal plasma of fertile men might be used as biomarkers of male fertility status. To test this concept, the present study examined the semen parameters of males among 14 normal idiopathic (normozoospermia) and 84 subnormal (teratozoospermia, oligoteratozoospermia, oligoasthenoteratozoospermia) infertile individuals\. We investigated levels of protein expression/activity of Cu, Zn superoxide dismutase (CuZnSOD), manganese superoxide dismutase (MnSOD), catalase and glutathione peroxidase (GSH-Px), their association with functional sperm ...
Source: Reproductive Biology - April 9, 2019 Category: Reproduction Medicine Authors: Otasevic V, Kalezic A, Macanovic B, Jankovic A, Stancic A, Garalejic E, Korac A, Korac B Tags: Syst Biol Reprod Med Source Type: research

Serum Antim üllerian hormone does not predict elevated progesterone levels among women who undergo controlled ovarian hyperstimulation for in vitro fertilization.
Serum Antimüllerian hormone does not predict elevated progesterone levels among women who undergo controlled ovarian hyperstimulation for in vitro fertilization. Reprod Biol Endocrinol. 2019 Apr 08;17(1):35 Authors: Kavoussi SK, Chen SH, Hunn CL, West BT, Wininger JD, Kavoussi KM, Kavoussi PK Abstract Serum Antimüllerian hormone (AMH) has been shown to predict various in vitro fertilization (IVF) outcomes. AMH and progesterone (P) are products of granulosa cells of the ovary. Since overall granulosa cell number directly correlates with oocyte number and AMH production, the aim of this study ...
Source: Reproductive Biology - April 8, 2019 Category: Reproduction Medicine Authors: Kavoussi SK, Chen SH, Hunn CL, West BT, Wininger JD, Kavoussi KM, Kavoussi PK Tags: Reprod Biol Endocrinol Source Type: research

Levels of circulating insulin cell-free DNA in women with polycystic ovary syndrome - a longitudinal cohort study.
CONCLUSION: The circulating level of unmethylated and methylated INScfDNA is similar between PCOS and Controls and cannot be used to predict islet β-cell loss and progression to Type 2 diabetes in a 6-year follow-up. TRIAL REGISTRATION: The Danish Data Protection Agency (REG-31-2016. Approval: 01-12-2015) and by the Danish Scientific Ethical committee of Region Zealand (Journal no. SJ-525. Approval: 13-06-2016), Clinicaltrials.gov, ( NCT03142633 , registered 1. March, 2017, Retrospectively registered). PMID: 30953560 [PubMed - in process] (Source: Reproductive Biology)
Source: Reproductive Biology - April 5, 2019 Category: Reproduction Medicine Authors: Udesen PB, Sørensen AE, Joglekar MV, Hardikar AA, Wissing MLM, Englund AM, Dalgaard LT Tags: Reprod Biol Endocrinol Source Type: research

Comparing early embryo morphokinetics with time-lapse microscopy in patients with low and normal ovarian response to ovarian stimulation.
ler A Abstract In this retrospective study, patients undergoing ovarian stimulation were allocated into two groups (47 normal responders (NR) vs 47 low responders (LR)) according to the number of aspirated oocytes and their Anti-Müllerian hormone (AMH). 171 oocytes were retrieved from the LR group and 447 oocytes from the NR group. The oocytes were studied regarding the early embryo morphokinetic parameters and cleavage stage patterns after data extraction from our EmbryoScope database. The following parameters were recorded; time of PN fading (tPNf), time to two cells (t2), three cells (t3), four cells (t4),...
Source: Reproductive Biology - March 30, 2019 Category: Reproduction Medicine Authors: Alexopoulou E, Pinborg A, Budtz-Jørgensen E, Zedeler A Tags: Reprod Biol Source Type: research

Dexamethasone Induces Primary Amnion Epithelial Cell Senescence through Telomere-P21 Associated Pathway.
CONCLUSION: Dex treatment of AECs produced non-replicative and non-inflammatory senescence. Extensive use of Dex during the perinatal period may lead to cellular senescence, contributing to cellular aging associated pathologies during the perinatal and neonatal periods. PMID: 30927408 [PubMed - as supplied by publisher] (Source: Reproductive Biology)
Source: Reproductive Biology - March 30, 2019 Category: Reproduction Medicine Authors: Martin LF, Richardson LS, da Silva MG, Sheller-Miller S, Menon R Tags: Biol Reprod Source Type: research

Formation of organotypic testicular organoids in microwell culture.
Abstract Three-dimensional (3D) organoids can serve as an in vitro platform to study cell-cell interactions, tissue development and toxicology. Development of organoids with tissue architecture similar to testis in vivo has remained a challenge. Here, we present a microwell aggregation approach to establish multicellular 3D testicular organoids from pig, mouse, macaque and human. The organoids consist of germ cells, Sertoli cells, Leydig cells and peritubular myoid cells forming a distinct seminiferous epithelium and interstitial compartment separated by a basement membrane. Sertoli cells in the organoids express ...
Source: Reproductive Biology - March 30, 2019 Category: Reproduction Medicine Authors: Sakib S, Uchida A, Valenzuela-Leon P, Yu Y, Valli-Pulaski H, Orwig K, Ungrin M, Dobrinski I Tags: Biol Reprod Source Type: research

Molecular profiling demonstrates modulation of immune cell function and matrix remodeling during luteal rescue †.
Molecular profiling demonstrates modulation of immune cell function and matrix remodeling during luteal rescue†. Biol Reprod. 2019 Mar 27;: Authors: Hughes CK, Maalouf SW, Liu WS, Pate JL Abstract The corpus luteum (CL) is essential for maintenance of pregnancy in all mammals and luteal rescue, which occurs around day 16-19 in the cow, is necessary to maintain luteal progesterone production. Transcriptomic and proteomic profiling were performed to compare the day 17 bovine CL of the estrous cycle and pregnancy. Among mRNA and proteins measured, 140 differentially abundant mRNA and 24 differenti...
Source: Reproductive Biology - March 27, 2019 Category: Reproduction Medicine Authors: Hughes CK, Maalouf SW, Liu WS, Pate JL Tags: Biol Reprod Source Type: research

GIT2 deficiency attenuates inflammation induced expression of pro-labor mediators in human amnion and myometrial cells.
Abstract Untimely activation of the inflammatory response by sterile or infective insults in uterine tissues can result in preterm birth. Pro-inflammatory cytokines and pathogenic activation of toll-like receptors (TLRs) initiates a biochemical cascade of events leading to myometrial activation and contractility, cervical dilatation, and rupture of the chorioamniotic membranes. GIT2 is a signaling protein known to play a role in innate and adaptive immunity; however, its role in the inflammatory pathways of human labor are not known. In this article, we report that GIT2 expression is lower in human myometrium and ...
Source: Reproductive Biology - March 27, 2019 Category: Reproduction Medicine Authors: Lim R, Lappas M Tags: Biol Reprod Source Type: research

MiR-664-2 impacts pubertal development in a precocious-puberty rat model through targeting the NMDA receptor-1.
Abstract Precocious puberty commonly results from premature activation of the hypothalamic-pituitary-gonadal axis (HPGA). Gonadotropin-releasing hormone (GnRH) is the initial trigger for HPGA activation and plays an important role in puberty onset. N-methyl-D-aspartate (NMDA) can promote pulsatile GnRH secretion and accelerates puberty onset. However, the mechanism of N-methyl-D-aspartate receptors (NMDARs) in precocious puberty (PP) pathogenesis remains obscure. We found that serum GnRH, luteinizing hormone (LH), follicle-stimulating hormone (FSH), estrogen (E2) levels, hypothalamic NMDAR1 and GnRH mRNA expressio...
Source: Reproductive Biology - March 27, 2019 Category: Reproduction Medicine Authors: Ju M, Yang L, Zhu J, Chen Z, Zhang M, Yu J, Tian Z Tags: Biol Reprod Source Type: research

Fetal phenotypes of congenital disorder of glycosylation: A case presentation.
PMID: 30910375 [PubMed - as supplied by publisher] (Source: Reproductive Biology)
Source: Reproductive Biology - March 19, 2019 Category: Reproduction Medicine Authors: Yang YD, Xu LL, Li DZ Tags: Eur J Obstet Gynecol Reprod Biol Source Type: research

Prenatal ultrasound diagnosis of fetal chest wall cystic lymphangioma: An Italian case series.
Abstract Fetal lymphangioma is a rare congenital malformation of lymphatic system that involve the skin and the subcutaneous tissue. The vast majority of the lymphangioma occurs in the neck. More rarely lymphangiomas may occur in the axillary region, including chest wall. Our consecutive case series study included three cases of fetal chest wall cystic lymphangiomas. In our cohort, fetal chest wall cystic lymphangiomas were the 18.8% of the all cases of lymphangiomas of axillary region. In all the three cases no other fetal abnormalities were evaluated, and the chest wall cystic lymphangiomas were unilateral, hone...
Source: Reproductive Biology - March 18, 2019 Category: Reproduction Medicine Authors: Saccone G, Di Meglio L, Di Meglio L, Zullo F, Locci M, Zullo F, Berghella V, Di Meglio A Tags: Eur J Obstet Gynecol Reprod Biol Source Type: research

Retraction notice to "Impact of vitrification on human oocytes before and after in vitro maturation: A systematic review and meta-analysis" [Eur. J. Obstet. Gynecol. Reprod. Biol. 227 (August) (2018), 19-26].
Retraction notice to "Impact of vitrification on human oocytes before and after in vitro maturation: A systematic review and meta-analysis" [Eur. J. Obstet. Gynecol. Reprod. Biol. 227 (August) (2018), 19-26]. Eur J Obstet Gynecol Reprod Biol. 2019 Mar 16;: Authors: Mohsenzadeh M, Salehi-Abargouei A, Tabibnejad N, Karimi-Zarchi M, Ali Khalili M PMID: 30902416 [PubMed - as supplied by publisher] (Source: Reproductive Biology)
Source: Reproductive Biology - March 16, 2019 Category: Reproduction Medicine Authors: Mohsenzadeh M, Salehi-Abargouei A, Tabibnejad N, Karimi-Zarchi M, Ali Khalili M Tags: Eur J Obstet Gynecol Reprod Biol Source Type: research

Expression of the plasminogen system in the physiological mouse ovary and in the pathological polycystic ovary syndrome (PCOS) state.
CONCLUSIONS: Our novel study, that comprehensively assessed the fibrinolytic/proteolytic system in the mouse ovary, showed the expression, differential localisation and a potential role for the plasminogen system in the physiological mouse ovary and in PCOS. Androgens may be involved in regulating expression of the ovarian plasminogen system. Further studies evaluating these markers at different time-points of ovulation may help to further clarify both physiological and potential pathological actions these markers play in ovulatory processes distorted in PCOS. PMID: 30878040 [PubMed - in process] (Source: Reproductive Biology)
Source: Reproductive Biology - March 16, 2019 Category: Reproduction Medicine Authors: Burchall GF, Pouniotis DS, Teede HJ, Ranasinha S, Walters KA, Piva TJ Tags: Reprod Biol Endocrinol Source Type: research

Epigenetic role of the nuclear factor NF-Y on ID gene family in endometrial tissues of women with endometriosis: a case control study.
Abstract BACKGROUND: A predominant difference between endometrial and normal cells is higher proliferation rate in the former cells which is benign. The genes of inhibitor of differentiation (ID) family play a major role in cell proliferation regulation which might be targeted by the nuclear transcription factor Y (NF-Y) for subsequent epigenetic modifications through the CCAAT box regulatory region. The present study was designed to investigate the epigenetic role of NF-Y on ID gene family in endometrial tissue of patients with endometriosis. MATERIALS & METHODS: In this case-control study, 20 patients w...
Source: Reproductive Biology - March 15, 2019 Category: Reproduction Medicine Authors: Amirteimouri S, Ashini M, Ramazanali F, Aflatoonian R, Afsharian P, Shahhoseini M Tags: Reprod Biol Endocrinol Source Type: research

Correction to: The role of recombinant LH in women with hypo-response to controlled ovarian stimulation: a systematic review and meta-analysis.
Abstract . PMID: 30871582 [PubMed - in process] (Source: Reproductive Biology)
Source: Reproductive Biology - March 14, 2019 Category: Reproduction Medicine Authors: Conforti A, Esteves SC, Di Rella F, Strina I, De Rosa P, Fiorenza A, Zullo F, De Placido G, Alviggi C Tags: Reprod Biol Endocrinol Source Type: research

Should ICSI be implemented during IVF to all advanced-age patients with non-male factor subfertility?
Abstract BACKGROUD: In order to maximize In vitro fertilization (IVF) success rates in advanced- age patients, it has been suggested to favor the use of intracytoplasmic sperm injection (ICSI) over conventional insemination (CI), with the notion that ICSI would serve as a tool to overcome interference in sperm oocyte interaction and sperm oocyte penetration issues that can be related to maternal age and are not due to sperm abnormalities. We therefore aim to evaluate the role of ICSI in the treatment of non-male factor infertile patients aged ≥35 in terms of fertilization and top-quality embryo rates. METH...
Source: Reproductive Biology - March 7, 2019 Category: Reproduction Medicine Authors: Farhi J, Cohen K, Mizrachi Y, Weissman A, Raziel A, Orvieto R Tags: Reprod Biol Endocrinol Source Type: research

Physiological and anatomical aspects of the reproduction of mice with reduced Syndecan-1 expression.
üst DM Abstract BACKGROUND: Syndecan-1 is a heparan sulfate proteoglycan acting as a co-receptor for cytokines and growth factors mediating developmental, immunological and angiogenic processes. In human, the uteroplacental localization of Syndecan-1 and its reduced expression in pregnancy-associated pathologies, such as the intrauterine growth restriction, suggests an influence of Syndecan-1 in embryo-maternal interactions. The aim of the present study was to identify the effect of a reduced expression of Syndecan-1 on the reproductive phenotype of mice and their progenies. METHODS: Reproductive charact...
Source: Reproductive Biology - March 2, 2019 Category: Reproduction Medicine Authors: Gougoula C, Bielfeld AP, Pour SJ, Krüssel JS, Götte M, Benten WPM, Baston-Büst DM Tags: Reprod Biol Endocrinol Source Type: research

Deciphering the molecular mechanism during doxorubicin-mediated oxidative stress, apoptosis through Nrf2 and PGC-1 α in a rat testicular milieu.
Deciphering the molecular mechanism during doxorubicin-mediated oxidative stress, apoptosis through Nrf2 and PGC-1α in a rat testicular milieu. Reprod Biol. 2019 Feb 28;: Authors: Renu K, Valsala Gopalakrishnan A Abstract Doxorubicin is an extensively applied anti-cancerous drug since 1950's and its usage is constrained because of its accumulation in a non-cancerous organ. Many studies have proven that doxorubicin causes reproductive toxicity depends on its dosage, particularly due to increased oxidative stress and apoptosis. A number of the researches have been carried out concerning its preven...
Source: Reproductive Biology - February 28, 2019 Category: Reproduction Medicine Authors: Renu K, Valsala Gopalakrishnan A Tags: Reprod Biol Source Type: research