Tranylcypromine, a lysine-specific demethylase 1 (LSD1) inhibitor, suppresses lesion growth and improves generalized hyperalgesia in mouse with induced endometriosis.
CONCLUSION: In light of our previously reported reduced cellular proliferation, cell cycle progression and invasiveness resulting from the LSD1 inhibition in in vitro studies, our data strongly suggest that LSD1 is a promising therapeutic target for endometriosis. TRIAL REGISTRATION: Not applicable. PMID: 27062244 [PubMed - in process] (Source: Reproductive Biology)
Source: Reproductive Biology - April 13, 2016 Category: Reproduction Medicine Authors: Sun Q, Ding D, Liu X, Guo SW Tags: Reprod Biol Endocrinol Source Type: research

Flutamide induces alterations in the cell-cell junction ultrastructure and reduces the expression of Cx43 at the blood-testis barrier with no disturbance in the rat seminiferous tubule morphology.
Abstract BACKGROUND: Present study was designed to establish a causal connection between changes in the cell-cell junction protein expression at the blood-testis barrier and alterations in the adult rat testis histology following an anti-androgen flutamide exposure. Particular emphasis was placed on the basal ectoplasmic specialization (ES) in the seminiferous epithelium and expression of gap junction protein, connexin 43 (Cx43). METHODS: Flutamide (50 mg/kg body weight) was administered to male rats daily from 82 to 88 postnatal day. Testes from 90-day-old control and flutamide-exposed rats were used fo...
Source: Reproductive Biology - April 4, 2016 Category: Reproduction Medicine Authors: Chojnacka K, Hejmej A, Zarzycka M, Tworzydlo W, Bilinski S, Pardyak L, Kaminska A, Bilinska B Tags: Reprod Biol Endocrinol Source Type: research

A logistic model to predict early pregnancy loss following in vitro fertilization based on 2601 infertility patients.
The objectives of this study were to investigate the differences in ultrasound parameters between a miscarriage group and an ongoing pregnancy group during the 1(st) trimester and to build a logistic model to predict early pregnancy loss (EPL) after the appearance of embryonic cardiac activity in patients who have undergone in vitro fertilization embryo transfer (IVF-ET) treatment. METHOD: A total of 2601 patients with early singleton pregnancies with embryonic cardiac activity were retrospectively analyzed after IVF from January 2010 to June 2011. Transvaginal sonography (TVS) was performed at 6 to 10 weeks of g...
Source: Reproductive Biology - April 4, 2016 Category: Reproduction Medicine Authors: Yi Y, Lu G, Ouyang Y, Lin G, Gong F, Li X Tags: Reprod Biol Endocrinol Source Type: research

Genetic mouse embryo assay: improving performance and quality testing for assisted reproductive technology (ART) with a functional bioassay.
CONCLUSIONS: This novel approach provides a superior MEA that is more meaningful and sensitive for detection of embryotoxicity than morphological assessment alone. PMID: 27009109 [PubMed - in process] (Source: Reproductive Biology)
Source: Reproductive Biology - March 26, 2016 Category: Reproduction Medicine Authors: Gilbert RS, Nunez B, Sakurai K, Fielder T, Ni HT Tags: Reprod Biol Endocrinol Source Type: research

Ectopic pregnancy risk factors for ART patients undergoing the GnRH antagonist protocol: a retrospective study.
CONCLUSION: Excessive ovarian response, IVF (as opposed to ICSI), prior Cesarean section and GnRH agonist trigger were found to be independent risk factors for ectopic pregnancy. Caution should be exercised before incorporating the GnRH agonist trigger for indications other than preventing OHSS. When excessive ovarian response leads to utilization of GnRH agonist trigger, strategies for preventing ectopic pregnancy, such as a freeze all policy or blastocyst transfer, should be considered. Further studies should elucidate whether adjusting the luteal support can reduce the ectopic pregnancy risk. PMID: 27005813 [PubMed...
Source: Reproductive Biology - March 25, 2016 Category: Reproduction Medicine Authors: Weiss A, Beck-Fruchter R, Golan J, Lavee M, Geslevich Y, Shalev E Tags: Reprod Biol Endocrinol Source Type: research

Relaxin deficiency results in increased expression of angiogenesis- and remodelling-related genes in the uterus of early pregnant mice but does not affect endometrial angiogenesis prior to implantation.
CONCLUSIONS: Relaxin treatment modulates expression of a variety of angiogenesis-related genes in HES cells. However, despite accelerated uterine gene expression of steroid receptor, progesterone and angiogenesis and extracellular matrix remodeling genes in Rln-/- mice, there was no impact on angiogenesis. We conclude that although relaxin deficiency results in phenotypic changes in the pre-implantation uterus, endogenous relaxin does not play a major role in pre-implantation angiogenesis in the mouse uterus. PMID: 27005936 [PubMed - in process] (Source: Reproductive Biology)
Source: Reproductive Biology - March 25, 2016 Category: Reproduction Medicine Authors: Marshall SA, Ng L, Unemori EN, Girling JE, Parry LJ Tags: Reprod Biol Endocrinol Source Type: research

[Emergency caesarean in type 1 maternity: Impact of using color code].
CONCLUSION: Medical management by in situ injection of methotrexate under sonographic guidance with an intramuscular injection within the 48hours following the in situ injection and mifepristone when ectopic pregnancy was active can be proposed in first-line therapy in case of unruptured interstitial pregnancy. This treatment has a great efficiency and low rate of complications. PMID: 26993077 [PubMed - as supplied by publisher] (Source: Reproductive Biology)
Source: Reproductive Biology - March 15, 2016 Category: Reproduction Medicine Authors: Debras E, Fernandez H, Pourcelot AG, Houllier M, Capmas P Tags: J Gynecol Obstet Biol Reprod (Paris) Source Type: research

Ovarian parameters and ovarian blood flow of women living in the area of environmental crisis.
CONCLUSION: This study suggests definite environmental effect on ovarian parameters as indicated by decreased AFC, decreased both follicular and luteal OV and OBF in women living in environmental crisis Shalkar group compared to Aktobe group. PMID: 26989802 [PubMed - as supplied by publisher] (Source: Reproductive Biology)
Source: Reproductive Biology - March 8, 2016 Category: Reproduction Medicine Authors: Balmagambetova A, Abdelazim IA, Bekmukhambetov E, Zhurabekova G, Yehia AH, AbuFaza M Tags: Eur J Obstet Gynecol Reprod Biol Source Type: research

Noninvasive prenatal testing: more caution in counseling is needed in high risk pregnancies with ultrasound abnormalities.
CONCLUSION: More education of health care providers and caution in counseling and interpretation of test results are needed in order to meet the challenges that this new test, which enriches our diagnostic options in prenatal testing, poses. PMID: 26989803 [PubMed - as supplied by publisher] (Source: Reproductive Biology)
Source: Reproductive Biology - March 8, 2016 Category: Reproduction Medicine Authors: Oneda B, Steindl K, Masood R, Reshetnikova I, Krejci P, Baldinger R, Reissmann R, Taralczak M, Guetg A, Wisser J, Fauchère JC, Rauch A Tags: Eur J Obstet Gynecol Reprod Biol Source Type: research

Selecting the embryo with the highest implantation potential using a data mining based prediction model.
Abstract BACKGROUND: Embryo selection has been based on developmental and morphological characteristics. However, the presence of an important intra-and inter-observer variability of standard scoring system (SSS) has been reported. A computer-assisted scoring system (CASS) has the potential to overcome most of these disadvantages associated with the SSS. The aims of this study were to construct a prediction model, with data mining approaches, and compare the predictive performance of models in SSS and CASS and to evaluate whether using the prediction model would impact the selection of the embryo for transfer. ...
Source: Reproductive Biology - March 5, 2016 Category: Reproduction Medicine Authors: Chen F, De Neubourg D, Debrock S, Peeraer K, D'Hooghe T, Spiessens C Tags: Reprod Biol Endocrinol Source Type: research

Repulsive Guidance Molecule b (RGMb) Is Dispensable for Normal Gonadal Function in Mice.
Abstract Bone morphogenetic protein (BMP) signaling plays an important role in spermatogenesis and follicle development. Our previous studies have shown that repulsive guidance molecule b (RGMb, also known as Dragon) is a co-receptor that enhances BMP2 and BMP4 signaling in several cell types, and that RGMb is expressed in spermatocytes and spermatids in the testis, and in oocytes of the secondary follicles in the ovary. Here, we demonstrated that specific deletion of Rgmb in germ cells in the testis and ovary did not alter Smad1/5/8 phosphorylation, gonadal structures and fertility. In addition, ovaries from post...
Source: Reproductive Biology - February 24, 2016 Category: Reproduction Medicine Authors: Meng C, Liu W, Huang H, Wang Y, Chen B, Freeman GJ, Schneyer A, Lin HY, Xia Y Tags: Biol Reprod Source Type: research

Identification of PIWIL1 Isoforms and Their Expression in Bovine Testes, Oocytes, and Early Embryos.
Abstract PIWI proteins are members of the larger Argonaute family and bind to specific 24-32 nucleotide (nt) RNAs called PIWI-interacting RNAs (piRNAs). piRNAs direct PIWI-mediated suppression of retrotransposon expression in the male germline in humans and mice but their roles in bovine reproduction and embryogenesis are unknown. Although the majority of research in mammals has focused on the functions of PIWI proteins during spermatogenesis, this family of proteins and their associated piRNAs have recently been identified in early embryos. The goals of this study were to characterize the expression of PIWIL1 in ...
Source: Reproductive Biology - February 24, 2016 Category: Reproduction Medicine Authors: Russell SJ, Stalker L, Gilchrist G, Backx A, Molledo G, Foster RA, LaMarre J Tags: Biol Reprod Source Type: research

Morphokinetic Evaluation of Embryo Development in a Mouse Model: Functional and Molecular Correlates.
Abstract Although time-lapse analysis of early embryo cleavage parameters (morphokinetics) predicts blastocyst development, it has not been definitively linked to establishing pregnancy and live birth. For example, a direct comparison of the developmental potential of embryos with optimal kinetic parameters compared to suboptimal kinetics has not been performed with human embryos. To ascertain whether such a linkage exists, we developed a mouse model of morphokinetic analysis of early embryo cleavage using time-lapse microscopy to predict blastocyst formation, and tested whether cleavage parameters predict pregnan...
Source: Reproductive Biology - February 24, 2016 Category: Reproduction Medicine Authors: Weinerman R, Feng R, Ord TS, Schultz RM, Bartolomei MS, Coutifaris C, Mainigi M Tags: Biol Reprod Source Type: research

The Effects of Chemotherapeutic Agents, Bleomycin, Etoposide, and Cisplatin, on Chromatin Remodeling in Male Germ Cells.
The objective of these studies was to investigate the effects of BEP on epigenetic modifications to histones involved in chromatin remodeling. Brown-Norway rats were treated with BEP, and their testes were removed to isolate pachytene spermatocytes and round spermatids by unit gravity sedimentation. Western blot analyses were conducted on extracted proteins to detect the expression of key modified histones. In a second cohort testes were prepared for immunohistochemical analysis. The stage-specific expression of each modified histone mark in rat spermatogenesis suggests the involvement of these modifications in chromatin r...
Source: Reproductive Biology - February 24, 2016 Category: Reproduction Medicine Authors: Bagheri-Sereshki N, Hales BF, Robaire B Tags: Biol Reprod Source Type: research

Next Generation Sequencing Based Comprehensive Chromosome Screening in Mouse Polar Bodies, Oocytes, and Embryos.
Abstract Advanced reproductive age is unequivocally associated with increased aneuploidy in human oocytes, which contributes to infertility, miscarriages, and birth defects. The frequency of meiotic chromosome segregation errors in oocytes derived from reproductively aged mice appear to be similar to those observed in humans, but a limitation of this important model system is our inability to accurately identify chromosome-specific aneuploidy. Here we report the validation and application of a new low pass whole genome sequencing approach to comprehensively screen chromosome aneuploidy in individual mouse oocytes ...
Source: Reproductive Biology - February 24, 2016 Category: Reproduction Medicine Authors: Treff NR, Krisher RL, Tao X, Garnsey H, Bohrer C, Silva E, Landis J, Taylor D, Scott RT, Woodruff TK, Duncan FE Tags: Biol Reprod Source Type: research

Germ Cell-Specific Excision of loxP-Flanked Transgenes in Rainbow Trout Oncorhynchus mykiss.
Abstract Cre/loxP-mediated DNA excision in germ cell lineages could contribute substantially to the study of germ cell biology in salmonids, which are emerging as a model species in this field. However, a cell type-specific Cre/loxP system has not been successfully developed for any salmonid species. Therefore, we examined the feasibility of Cre/loxP-mediated, germ cell-specific gene excision and transgene activation in rainbow trout. Double-transgenic (wTg) progeny were obtained by mating a transgenic male carrying cre with a transgenic female carrying the hsc-LRLG gene; cre was driven by rainbow trout vasa regul...
Source: Reproductive Biology - February 24, 2016 Category: Reproduction Medicine Authors: Katayama N, Kume S, Hattori-Ihara S, Sadaie S, Hayashi M, Yoshizaki G Tags: Biol Reprod Source Type: research

New Insights into the Role of Autophagy in Ovarian Cryopreservation by Vitrification.
Abstract Ovarian cryopreservation by vitrification is a highly useful method for preserving female fertility during radiotherapy and chemotherapy. However, cryo-injury, osmotic stress during vitrification, and ischemia/reperfusion during transplantation lead to losses of ovarian follicles. Ovarian follicle loss may be partially reduced by several methods; however, studies regarding the mechanism of ovarian follicle loss have only investigated cell apoptosis, which comprises type I programmed cell death. Autophagy comprises type II programmed cell death, and cell homeostasis is maintained by autophagy during condit...
Source: Reproductive Biology - February 24, 2016 Category: Reproduction Medicine Authors: Yang Y, Cheung HH, Law WN, Zhang C, Chan WY, Pei X, Wang Y Tags: Biol Reprod Source Type: research

Dehydroepiandrosterone plus climen supplementation shows better effects than dehydroepiandrosterone alone on infertility patients with diminished ovarian reserve of low-FSH level undergoing in-vitro fertilization cycles: a randomized controlled trial.
CONCLUSIONS: These observations suggest that patients with DOR of a low-FSH level might benefit more from DHEA plus climen supplementation than from DHEA supplementation alone. PMID: 26879683 [PubMed - in process] (Source: Reproductive Biology)
Source: Reproductive Biology - February 19, 2016 Category: Reproduction Medicine Authors: Zhang H, Chu Y, Zhou P, He X, Xu Q, Zhang Z, Cao Y, Wei Z Tags: Reprod Biol Endocrinol Source Type: research

Assessment of the Access AMH assay as an automated, high-performance replacement for the AMH Generation II manual ELISA.
CONCLUSION: Here, we verified the published performance of the Access AMH assay and showed excellent correlation with the Gen II ELISA method. Moreover, we validated this correlation by confirming that the results from a fertile AMH reference range established using the preceding Gen II ELISA are interchangeable with the new automated Access AMH assay. PMID: 26879773 [PubMed - in process] (Source: Reproductive Biology)
Source: Reproductive Biology - February 19, 2016 Category: Reproduction Medicine Authors: Pearson K, Long M, Prasad J, Wu YY, Bonifacio M Tags: Reprod Biol Endocrinol Source Type: research

Measurement of betamethasone concentration in maternal serum treated for fetal lung maturity; Is it feasible?
Abstract BACKGROUND: The association between maternal serum concentration of betamethasone given for fetal lung maturity and perinatal outcome has not been investigated. This may be due to an absence of a reliable method for measuring serum betamethasone concentrations. We aimed in the current study to assess the feasibility of a specific ELISA kit to measure the concentrations of betamethasone in maternal serum and to examine the trend of sequential measurements after a course of betamethasone for fetal lung maturity. METHODS: Pregnant women at risk for preterm birth who received betamethasone between 24 and...
Source: Reproductive Biology - February 11, 2016 Category: Reproduction Medicine Authors: Salim R, Suleiman A, Colodner R, Nachum Z, Goldstein LH, Shalev E Tags: Reprod Biol Endocrinol Source Type: research

Association of TNF-α genetic polymorphisms with recurrent pregnancy loss risk: a systematic review and meta-analysis.
CONCLUSIONS: The current meta-analysis demonstrates that TNF-α-308G/A polymorphism in the TNF-α gene is associated with susceptibility to RPL. PMID: 26837816 [PubMed - in process] (Source: Reproductive Biology)
Source: Reproductive Biology - February 4, 2016 Category: Reproduction Medicine Authors: Li HH, Xu XH, Tong J, Zhang KY, Zhang C, Chen ZJ Tags: Reprod Biol Endocrinol Source Type: research

Post-thaw ATP supplementation enhances cryoprotective effect of iodixanol in rat spermatozoa.
CONCLUSIONS: Iodixanol has cryoprotective effects during rat sperm freezing without any toxic effect. Moreover, the combinations of iodixanol and ATP have a beneficial role in maintaining function of frozen-thawed rat spermatozoa for long period of incubation post-thaw. PMID: 26821719 [PubMed - in process] (Source: Reproductive Biology)
Source: Reproductive Biology - February 1, 2016 Category: Reproduction Medicine Authors: Kim S, Hooper S, Agca C, Agca Y Tags: Reprod Biol Endocrinol Source Type: research

Anti-Müllerian hormone serum level and other markers associated with pregnancy outcome in oocyte donation.
CONCLUSION: Serum AMH level is associated with pregnancy outcome after oocyte donation. PMID: 26787450 [PubMed - in process] (Source: Reproductive Biology)
Source: Reproductive Biology - January 22, 2016 Category: Reproduction Medicine Authors: Delesalle AS, Robin G, Thomas-Desrousseaux P, Dewailly D, Catteau-Jonard S Tags: Reprod Biol Endocrinol Source Type: research

Factors affecting pregnancy outcomes in young women treated with fertility-preserving therapy for well-differentiated endometrial cancer or atypical endometrial hyperplasia.
CONCLUSIONS: We identified three factors considered to affect pregnancy establishment following conservative treatment with MPA: recurrence, endometrial thickness during ovulation, and the age of the pregnancy permission. Introduction of infertility treatment including assisted reproductive technology (ART) soon after achieving tumor disappearance by MPA would therefore be beneficial for patients with disease recurrence, thin endometrium, or a higher age of pregnancy permission. PMID: 26769300 [PubMed - in process] (Source: Reproductive Biology)
Source: Reproductive Biology - January 18, 2016 Category: Reproduction Medicine Authors: Inoue O, Hamatani T, Susumu N, Yamagami W, Ogawa S, Takemoto T, Hirasawa A, Banno K, Kuji N, Tanaka M, Aoki D Tags: Reprod Biol Endocrinol Source Type: research

Effect of bone morphogenetic protein-4 on in vitro growth, steroidogenesis and subsequent developmental competence of the oocyte-granulosa cell complex derived from bovine early antral follicles.
Abstract BACKGROUND: Bone morphogenetic proteins (BMPs) play important regulatory roles during folliculogenesis. Theca-derived BMP-4 may be beneficial to in vitro growth culture of early antral follicle-derived oocyte-granulosa cell complexes (OGCs), which is lacking in theca-derived products. METHODS: BMP-4 (0 [control], 10 and 50 ng/mL) was added to growth culture medium. Growth, steroidogenesis and the subsequent developmental competence of OGCs derived from bovine early antral follicles (0.5-1 mm) were examined. RESULTS: At 4, 8 and 12 days of growth culture, progesterone production by...
Source: Reproductive Biology - January 18, 2016 Category: Reproduction Medicine Authors: Yang Y, Kanno C, Huang W, Kang SS, Yanagawa Y, Nagano M Tags: Reprod Biol Endocrinol Source Type: research

Cryopreservation of testis tissues and in vitro spermatogenesis.
Abstract Cancer treatments, either chemo- or radiotherapy, may cause severe damage to gonads which could lead to the infertility of patients. In post-pubertal male patients, semen cryopreservation is recommended to preserve the potential to have their own biological children in the future; however, it is not applicable to prepubertals. The preservation of testis tissue which contains spermatogonial stem cells (SSCs) but not sperm would be an alternative measure. The tissues or SSCs have to be transplanted back into patients to obtain sperm; however, this procedure remains experimental, invasive, and is accompanied...
Source: Reproductive Biology - January 16, 2016 Category: Reproduction Medicine Authors: Yokonishi T, Ogawa T Tags: Reprod Med Biol Source Type: research

Derivation of integration-free iPSCs from a Klinefelter syndrome patient.
CONCLUSIONS: KS patient-derived iPS cells that could differentiate into cardiomyocyte-like cells were established. PMID: 26709348 [PubMed - as supplied by publisher] (Source: Reproductive Biology)
Source: Reproductive Biology - January 16, 2016 Category: Reproduction Medicine Authors: Shimizu T, Shiohara M, Tai T, Nagao K, Nakajima K, Kobayashi H Tags: Reprod Med Biol Source Type: research

Randomized, active-controlled, comparative phase 3 efficacy and safety equivalence trial of Ovaleap® (recombinant human follicle-stimulating hormone) in infertile women using assisted reproduction technology (ART).
CONCLUSIONS: Ovaleap® has shown the same efficacy and safety as Gonal-f® for stimulation of follicular development in infertile women (up to 37 years of age) who are undergoing ART therapy. TRIAL REGISTRATION: EudraCT: 2009-017674-20. Current controlled trials: ISRCTN74772901 . Date of trial registration: 19 March 2010. PMID: 26733057 [PubMed - in process] (Source: Reproductive Biology)
Source: Reproductive Biology - January 16, 2016 Category: Reproduction Medicine Authors: Strowitzki T, Kuczynski W, Mueller A, Bias P Tags: Reprod Biol Endocrinol Source Type: research

Regulation of TIMP-1 in Human Placenta and Fetal Membranes by lipopolysaccharide and demethylating agent 5-aza-2'-deoxycytidine.
CONCLUSIONS: Collectively, these observations support a potential role for pharmacological agents that modify chromatin structure to be utilized in the therapeutic targeting of TIMP-1 to prevent premature rupture of the fetal membranes in an infectious setting. PMID: 26691525 [PubMed - in process] (Source: Reproductive Biology)
Source: Reproductive Biology - December 26, 2015 Category: Reproduction Medicine Authors: Vincent ZL, Mitchell MD, Ponnampalam AP Tags: Reprod Biol Endocrinol Source Type: research

Role of Anti-Müllerian Hormone in pathophysiology, diagnosis and treatment of Polycystic Ovary Syndrome: a review.
Role of Anti-Müllerian Hormone in pathophysiology, diagnosis and treatment of Polycystic Ovary Syndrome: a review. Reprod Biol Endocrinol. 2015;13(1):137 Authors: Dumont A, Robin G, Catteau-Jonard S, Dewailly D Abstract Polycystic ovary syndrome (PCOS) is the most common cause of chronic anovulation and hyperandrogenism in young women. Excessive ovarian production of Anti-Müllerian Hormone, secreted by growing follicles in excess, is now considered as an important feature of PCOS. The aim of this review is first to update the current knowledge about the role of AMH in the pathophysiology of ...
Source: Reproductive Biology - December 26, 2015 Category: Reproduction Medicine Authors: Dumont A, Robin G, Catteau-Jonard S, Dewailly D Tags: Reprod Biol Endocrinol Source Type: research

Parturition dysfunction in obesity: time to target the pathobiology.
We present evidence from human and animal studies of interactions between obesity and parturition signaling in all elements of the birth process, including: delayed cervical ripening, prostaglandin insensitivity, amniotic membrane strengthening, decreased myometrial oxytocin receptor expression, decreased myocyte action potential initiation and contractility, decreased myocyte gap junction formation, and impaired myocyte neutralization of reactive oxygen species. We found convincing clinical data on the effect of obesity on labor initiation and successful delivery, but few studies on the underlying pathobiology. We suggest...
Source: Reproductive Biology - December 22, 2015 Category: Reproduction Medicine Authors: Carlson NS, Hernandez TL, Hurt KJ Tags: Reprod Biol Endocrinol Source Type: research

The effect of cigarette smoking, alcohol consumption and fruit and vegetable consumption on IVF outcomes: a review and presentation of original data.
Abstract BACKGROUND: Lifestyle factors including cigarette smoking, alcohol consumption and nutritional habits impact on health, wellness, and the risk of chronic diseases. In the areas of in-vitro fertilization (IVF) and pregnancy, lifestyle factors influence oocyte production, fertilization rates, pregnancy and pregnancy loss, while chronic, low-grade oxidative stress may underlie poor outcomes for some IVF cases. METHODS: Here, we review the current literature and present some original, previously unpublished data, obtained from couples attending the PIVET Medical Centre in Western Australia. RESULTS:...
Source: Reproductive Biology - December 19, 2015 Category: Reproduction Medicine Authors: Firns S, Cruzat VF, Keane KN, Joesbury KA, Lee AH, Newsholme P, Yovich JL Tags: Reprod Biol Endocrinol Source Type: research

17β-estradiol prevents experimentally-induced oxidative damage to membrane lipids and nuclear DNA in porcine ovary.
17β-estradiol prevents experimentally-induced oxidative damage to membrane lipids and nuclear DNA in porcine ovary. Syst Biol Reprod Med. 2015 Dec 17;:1-5 Authors: Stepniak J, Karbownik-Lewinska M Abstract Estrogens, with their principle representative 17β-estradiol, contribute to the redox state of cells showing both pro- and antioxidative properties. In the ovary, being the main source of estrogens, maintaining balance between the production and detoxification of ROS is crucial. Whereas ovary estrogen concentration is difficult to estimate, its circulating concentration in women may reach ...
Source: Reproductive Biology - December 17, 2015 Category: Reproduction Medicine Authors: Stepniak J, Karbownik-Lewinska M Tags: Syst Biol Reprod Med Source Type: research

Does sperm DNA fragmentation affect the developmental potential and the incidence of apoptosis following blastomere biopsy?
Abstract Common methods employed in assisted reproduction technology (ART) include intracytoplasmic sperm injection (ICSI) with an unspecified level of sperm DNA fragmentation (SDF) and preimplantation genetic diagnosis (PGD). The aim of this study was to investigate the impact of SDF on human preimplantation embryo development and the incidence of apoptosis following a single blastomere biopsy. Using sperm chromatin dispersion (SCD) to assess SDF, a total of 20 processed semen samples were categorized into two groups; group I: SDF ≤30% and group II: SDF>30%. After ICSI, fertilization, cleavage, and embryo q...
Source: Reproductive Biology - December 17, 2015 Category: Reproduction Medicine Authors: Haghpanah T, Salehi M, Ghaffari Novin M, Masteri Farahani R, Fadaei-Fathabadi F, Dehghani-Mohammadabadi M, Azimi H Tags: Syst Biol Reprod Med Source Type: research

Key Signaling Events for Committing Mouse Pluripotent Stem Cells to the Germline Fate.
In this study, we primarily focus on the mouse model to discuss key signaling events for germline induction. We review mechanisms of competent regulators on primordial germ cell induction and discuss current achievements and difficulties in inducing sex-specific germline development. Furthermore, we review the developmental identities of mouse embryonic stem cells and epiblast stem cells under certain defined culture conditions as it relates to the differentiation process to become germline cells. PMID: 26674564 [PubMed - as supplied by publisher] (Source: Reproductive Biology)
Source: Reproductive Biology - December 16, 2015 Category: Reproduction Medicine Authors: Wang JQ, Cao WG Tags: Biol Reprod Source Type: research

CORRECTION: Effects of Synchronous and Asynchronous Embryo Transfer on Postnatal Development, Adult Health, and Behavior in Mice.
a F, Orio L, Gutierrez-Adan A Abstract CorrectionAngela P. López-Cardona, Raúl Fernández-González, Miriam Pérez-Crespo, Francisco Alén, Fernando Rodriguez de Fonseca, Laura Orio, and Alfonso Gutierrez-Adan. Effects of Synchronous and Asynchronous Embryo Transfer on Postnatal Development, Adult Health, and Behavior in Mice. Biol Reprod 2015; 93:85, 1-6. DOI:10.1095/biolreprod.115.130385The manuscript referenced above inadvertently listed only one corresponding author. The correct presentation of the author affiliations and corresponding authors appears below.Angela P. L&oac...
Source: Reproductive Biology - December 16, 2015 Category: Reproduction Medicine Authors: López-Cardona AP, Fernández-González R, Pérez-Crespo M, Alén F, Rodriguez de Fonseca F, Orio L, Gutierrez-Adan A Tags: Biol Reprod Source Type: research

The Transcription Factor Interferon Regulatory Factor-1 (IRF1) Plays a Key Role in the Terminal Effector Pathways of Human Preterm Labor.
In conclusion, IRF1 upregulation in fetal membranes and myometrium after term labor indicates a pro-inflammatory role for IRF1 in human parturition. IRF1 is involved in TLR and cytokine mediated signaling in human myometrium. These data provide new insights into the mechanisms associated with inflammation- and infection-associated preterm birth. IRF1 inhibitors as therapeutics for the management of spontaneous preterm birth warrants further investigation. PMID: 26674566 [PubMed - as supplied by publisher] (Source: Reproductive Biology)
Source: Reproductive Biology - December 16, 2015 Category: Reproduction Medicine Authors: Lim R, Tran HT, Liong S, Barker G, Lappas M Tags: Biol Reprod Source Type: research

Comparative Proteomic, Physiological, Morphological and Biochemical Analyses Reveal the Characteristics of the Diploid Spermatozoa of Allotetraploid Hybrids of Carassius auratus and Common Carp (Cyprinus carpio).
Abstract The generation of diploid spermatozoa is essential for the continuity of tetraploid lineages. The DNA content of diploid spermatozoa from allotetraploid hybrids of red crucian carp and common carp was nearly twice as great as that of haploid spermatozoa from common carp, and the durations of rapid and slow progressive motility were longer. We performed comparative proteomic analyses to measure variations in protein composition between diploid and haploid spermatozoa. Using two-dimensional electrophoresis followed by LC-MS/MS, 21 protein spots that changed in abundance were analyzed. As common carp and the...
Source: Reproductive Biology - December 16, 2015 Category: Reproduction Medicine Authors: Duan W, Xu K, Hu F, Zhang Y, Wen M, Wang J, Tao M, Luo K, Zhao R, Qin Q, Zhang C, Liu J, Liu Y, Liu S Tags: Biol Reprod Source Type: research

A global perspective on assisted reproductive technology fertility treatment: an 8-country fertility specialist survey.
CONCLUSIONS: This study, which follows a rigorous survey methodology, elucidates the current state of fertility specialists' practices and perspectives on the global fertility treatment field, which highlights differences and similarities among countries. This research may inform further studies and procedural developments that might better improve and standardize ART. PMID: 26645803 [PubMed - in process] (Source: Reproductive Biology)
Source: Reproductive Biology - December 13, 2015 Category: Reproduction Medicine Authors: Audibert C, Glass D Tags: Reprod Biol Endocrinol Source Type: research

Dorsal and medial raphe nuclei participate differentially in reproductive functions of the male rat.
CONCLUSIONS: The DRN but not the MRN regulates the secretion of gonadotropins and testicular function. PMID: 26643556 [PubMed - in process] (Source: Reproductive Biology)
Source: Reproductive Biology - December 9, 2015 Category: Reproduction Medicine Authors: Ayala ME, Velázquez DE, Mendoza JL, Monroy J, Domínguez R, Cárdenas M, Aragón A Tags: Reprod Biol Endocrinol Source Type: research

Phase I, two-way, crossover study to demonstrate bioequivalence and to compare safety and tolerability of single-dose XM17 vs Gonal-f(®) in healthy women after follicle-stimulating hormone downregulation.
CONCLUSIONS: PK bioequivalence of single 300-IU sc doses of XM17 to the reference product Gonal-f(®) was statistically demonstrated. XM17 was well tolerated both systemically and locally. TRIAL REGISTRATION: ClinicalTrials.gov: NCT02592031 ; date of registration: 28 October, 2015. PMID: 26621118 [PubMed - in process] (Source: Reproductive Biology)
Source: Reproductive Biology - December 4, 2015 Category: Reproduction Medicine Authors: Lammerich A, Mueller A, Bias P Tags: Reprod Biol Endocrinol Source Type: research

Difference in expression between AQP1 and AQP5 in porcine endometrium and myometrium in response to steroid hormones, oxytocin, arachidonic acid, forskolin and cAMP during the mid-luteal phase of the estrous cycle and luteolysis.
CONCLUSIONS: These results seem to indicate that uterine tissues; endometrium and myometrium, exhibit their own AQP expression profiles in response to examined factors. Moreover, the responses of AQP1/AQP5 at mRNA and protein levels to the studied factors in the endometrium and myometrium are more pronounced during luteolysis. This suggests that the above effects of the studied factors are connected with morphological and physiological changes taking place in the pig uterus during the estrous cycle. PMID: 26625724 [PubMed - in process] (Source: Reproductive Biology)
Source: Reproductive Biology - December 4, 2015 Category: Reproduction Medicine Authors: Skowronska A, Mlotkowska P, Nielsen S, Skowronski MT Tags: Reprod Biol Endocrinol Source Type: research

Novel methods of treating ovarian infertility in older and POF women, testicular infertility, and other human functional diseases.
Abstract In vitro maturation (IVM) and in vitro fertilization (IVF) technologies are facing with growing demands of older women to conceive. Although ovarian stem cells (OSCs) of older women are capable of producing in vitro fresh oocyte-like cells (OLCs), such cells cannot respond to IVM and IVF due to the lack of granulosa cells required for their maturation. Follicular renewal is also dependent on support of circulating blood mononuclear cells. They induce intermediary stages of meiosis (metaphase I chromosomal duplication and crossover, and telophase) in emerging ovarian germ cells, as for the first time demon...
Source: Reproductive Biology - March 19, 2015 Category: Reproduction Medicine Authors: Bukovsky A Tags: Reprod Biol Endocrinol Source Type: research

Acknowledgement of top manuscript reviewers 2014.
Abstract CONTRIBUTING REVIEWERS: The editor of Reproductive Biology and Endocrinology would like to thank all of our reviewers and particularly our top reviewers who have contributed to the journal in Volume 12 (2014). PMID: 25778478 [PubMed - in process] (Source: Reproductive Biology)
Source: Reproductive Biology - March 19, 2015 Category: Reproduction Medicine Authors: Bukovsky A Tags: Reprod Biol Endocrinol Source Type: research

Leptin in the canine uterus and placenta: possible implications in pregnancy.
CONCLUSIONS: Lep and LepR are differentially expressed in the canine uterus and placenta during pregnancy, and their presence in various cell types indicates paracrine/autocrine roles. The Lep signaling system may be one of the pathways involved in feto-maternal cross-talk, implantation and maintenance of pregnancy, and may have a regulatory role around parturition. PMID: 25778479 [PubMed - in process] (Source: Reproductive Biology)
Source: Reproductive Biology - March 19, 2015 Category: Reproduction Medicine Authors: Balogh O, Staub LP, Gram A, Boos A, Kowalewski MP, Reichler IM Tags: Reprod Biol Endocrinol Source Type: research

Progesterone, estradiol, arachidonic acid, oxytocin, forskolin and cAMP influence on aquaporin 1 and 5 expression in porcine uterine explants during the mid-luteal phase of the estrous cycle and luteolysis: an in vitro study.
CONCLUSIONS: This study documented specific patterns of AQP1 and AQP5 expression in response to P4, E2, AA, FSK and cAMP, thereby providing new indirect evidence of their role in maintaining the local fluid balance within the uterus during the mid-luteal phase of the estrous cycle and luteolysis in pigs. PMID: 25778480 [PubMed - in process] (Source: Reproductive Biology)
Source: Reproductive Biology - March 19, 2015 Category: Reproduction Medicine Authors: Skowronska A, Młotkowska P, Wojciechowicz B, Okrasa S, Nielsen S, Skowronski MT Tags: Reprod Biol Endocrinol Source Type: research

Human embryonic stem cell cultivation: historical perspective and evolution of xeno-free culture systems.
Abstract Human embryonic stem cells (hESC) have emerged as attractive candidates for cell-based therapies that are capable of restoring lost cell and tissue function. These unique cells are able to self-renew indefinitely and have the capacity to differentiate in to all three germ layers (ectoderm, endoderm and mesoderm). Harnessing the power of these pluripotent stem cells could potentially offer new therapeutic treatment options for a variety of medical conditions. Since the initial derivation of hESC lines in 1998, tremendous headway has been made in better understanding stem cell biology and culture requiremen...
Source: Reproductive Biology - March 19, 2015 Category: Reproduction Medicine Authors: Desai N, Rambhia P, Gishto A Tags: Reprod Biol Endocrinol Source Type: research

Placental fibroblast growth factor 21 is not altered in late-onset preeclampsia.
CONCLUSIONS: Late-onset PE is not associated with major changes to the expression of FGF21, its receptors or metabolic targets. PMID: 25778482 [PubMed - in process] (Source: Reproductive Biology)
Source: Reproductive Biology - March 19, 2015 Category: Reproduction Medicine Authors: Dekker Nitert M, Scholz-Romero K, Kubala MH, McIntyre HD, Callaway LK, Barrett HL Tags: Reprod Biol Endocrinol Source Type: research

Major protein alterations in spermatozoa from infertile men with unilateral varicocele.
CONCLUSIONS: The expression of these proteins of interest are altered and possibly functionally compromised in infertile men with unilateral varicocele. If validated, these proteins may lead to potential biomarker(s) and help better understand the mechanism involved in the pathophysiology of unilateral varicocele in infertile men. PMID: 25778483 [PubMed - in process] (Source: Reproductive Biology)
Source: Reproductive Biology - March 19, 2015 Category: Reproduction Medicine Authors: Agarwal A, Sharma R, Durairajanayagam D, Ayaz A, Cui Z, Willard B, Gopalan B, Sabanegh E Tags: Reprod Biol Endocrinol Source Type: research

Characterization and possible function of glyceraldehyde-3-phosphate dehydrogenase-spermatogenic protein GAPDHS in mammalian sperm.
CONCLUSION: GAPDHS is a sperm-specific glycolytic enzyme involved in energy production during spermatogenesis and sperm motility; its role in the sperm head is unknown. In this study, we identified the antigen with Hs8 antibody and confirmed its localization in the apical part of the sperm head in addition to the principal piece of the flagellum. In an indirect binding assay, we confirmed the potential role of GAPDHS as a binding protein that is involved in the secondary sperm/oocyte binding. PMID: 25778484 [PubMed - in process] (Source: Reproductive Biology)
Source: Reproductive Biology - March 19, 2015 Category: Reproduction Medicine Authors: Margaryan H, Dorosh A, Capkova J, Manaskova-Postlerova P, Philimonenko A, Hozak P, Peknicova J Tags: Reprod Biol Endocrinol Source Type: research