Maternal heterozygous mutation in CHEK1 leads to mitotic arrest in human zygotes
Protein Cell. 2021 May 4. doi: 10.1007/s13238-021-00844-9. Online ahead of print.NO ABSTRACTPMID:33948904 | DOI:10.1007/s13238-021-00844-9 (Source: Protein and Cell)
Source: Protein and Cell - May 5, 2021 Category: Cytology Authors: Beili Chen Jianying Guo Ting Wang Qianhui Lee Jia Ming Fangfang Ding Haitao Li Zhiguo Zhang Lin Li Yunxia Cao Jie Na Source Type: research

Homology-based repair induced by CRISPR-Cas nucleases in mammalian embryo genome editing
Protein Cell. 2021 May 4. doi: 10.1007/s13238-021-00838-7. Online ahead of print.ABSTRACTRecent advances in genome editing, especially CRISPR-Cas nucleases, have revolutionized both laboratory research and clinical therapeutics. CRISPR-Cas nucleases, together with the DNA damage repair pathway in cells, enable both genetic diversification by classical non-homologous end joining (c-NHEJ) and precise genome modification by homology-based repair (HBR). Genome editing in zygotes is a convenient way to edit the germline, paving the way for animal disease model generation, as well as human embryo genome editing therapy for some ...
Source: Protein and Cell - May 4, 2021 Category: Cytology Authors: Xiya Zhang Tao Li Jianping Ou Junjiu Huang Puping Liang Source Type: research

Convergent evolution of SARS-CoV-2 in human and animals
Protein Cell. 2021 Apr 30:1-4. doi: 10.1007/s13238-021-00847-6. Online ahead of print.NO ABSTRACTPMID:33929695 | PMC:PMC8085470 | DOI:10.1007/s13238-021-00847-6 (Source: Protein and Cell)
Source: Protein and Cell - April 30, 2021 Category: Cytology Authors: Hang-Yu Zhou Cheng-Yang Ji Hang Fan Na Han Xiao-Feng Li Aiping Wu Cheng-Feng Qin Source Type: research

Convergent evolution of SARS-CoV-2 in human and animals
Protein Cell. 2021 Apr 30. doi: 10.1007/s13238-021-00847-6. Online ahead of print.NO ABSTRACTPMID:33929695 | DOI:10.1007/s13238-021-00847-6 (Source: Protein and Cell)
Source: Protein and Cell - April 30, 2021 Category: Cytology Authors: Hang-Yu Zhou Cheng-Yang Ji Hang Fan Na Han Xiao-Feng Li Aiping Wu Cheng-Feng Qin Source Type: research

Molecular deconvolution of the neutralizing antibodies induced by an inactivated SARS-CoV-2 virus vaccine
Protein Cell. 2021 Apr 28:1-6. doi: 10.1007/s13238-021-00840-z. Online ahead of print.NO ABSTRACTPMID:33909239 | PMC:PMC8080482 | DOI:10.1007/s13238-021-00840-z (Source: Protein and Cell)
Source: Protein and Cell - April 28, 2021 Category: Cytology Authors: Xingdong Zhou Hui Wang Qun Ji Mingjuan Du Yuexia Liang Huanhuan Li Fan Li Hang Shang Xiujuan Zhu Wei Wang Lichun Jiang Alexey V Stepanov Tianyu Ma Nanxin Gong Xiaodong Jia Alexander G Gabibov Zhiyong Lou Yinying Lu Yu Guo Hongkai Zhang Xiaoming Yang Source Type: research

Molecular deconvolution of the neutralizing antibodies induced by an inactivated SARS-CoV-2 virus vaccine
Protein Cell. 2021 Apr 28. doi: 10.1007/s13238-021-00840-z. Online ahead of print.NO ABSTRACTPMID:33909239 | DOI:10.1007/s13238-021-00840-z (Source: Protein and Cell)
Source: Protein and Cell - April 28, 2021 Category: Cytology Authors: Xingdong Zhou Hui Wang Qun Ji Mingjuan Du Yuexia Liang Huanhuan Li Fan Li Hang Shang Xiujuan Zhu Wei Wang Lichun Jiang Alexey V Stepanov Tianyu Ma Nanxin Gong Xiaodong Jia Alexander G Gabibov Zhiyong Lou Yinying Lu Yu Guo Hongkai Zhang Xiaoming Yang Source Type: research

A good gut feeling on Sarm1-mediated axon degeneration
Protein Cell. 2021 Apr 26. doi: 10.1007/s13238-021-00842-x. Online ahead of print.NO ABSTRACTPMID:33900577 | DOI:10.1007/s13238-021-00842-x (Source: Protein and Cell)
Source: Protein and Cell - April 26, 2021 Category: Cytology Authors: Victoria E von Saucken David J Simon Source Type: research

Ferroptosis, radiotherapy, and combination therapeutic strategies
Protein Cell. 2021 Apr 23. doi: 10.1007/s13238-021-00841-y. Online ahead of print.ABSTRACTFerroptosis, an iron-dependent form of regulated cell death driven by peroxidative damages of polyunsaturated-fatty-acid-containing phospholipids in cellular membranes, has recently been revealed to play an important role in radiotherapy-induced cell death and tumor suppression, and to mediate the synergy between radiotherapy and immunotherapy. In this review, we summarize known as well as putative mechanisms underlying the crosstalk between radiotherapy and ferroptosis, discuss the interactions between ferroptosis and other forms of ...
Source: Protein and Cell - April 23, 2021 Category: Cytology Authors: Guang Lei Chao Mao Yuelong Yan Li Zhuang Boyi Gan Source Type: research

Molecular basis and homeostatic regulation of Zinc taste
Protein Cell. 2021 Apr 23. doi: 10.1007/s13238-021-00845-8. Online ahead of print.NO ABSTRACTPMID:33891304 | DOI:10.1007/s13238-021-00845-8 (Source: Protein and Cell)
Source: Protein and Cell - April 23, 2021 Category: Cytology Authors: Rui Luo Yuxiang Zhang Yinjun Jia Yan Zhang Zongyang Li Jieqing Zhao Ting Liu Wei Zhang Source Type: research

Ferroptosis, radiotherapy, and combination therapeutic strategies
Protein Cell. 2021 Apr 23. doi: 10.1007/s13238-021-00841-y. Online ahead of print.ABSTRACTFerroptosis, an iron-dependent form of regulated cell death driven by peroxidative damages of polyunsaturated-fatty-acid-containing phospholipids in cellular membranes, has recently been revealed to play an important role in radiotherapy-induced cell death and tumor suppression, and to mediate the synergy between radiotherapy and immunotherapy. In this review, we summarize known as well as putative mechanisms underlying the crosstalk between radiotherapy and ferroptosis, discuss the interactions between ferroptosis and other forms of ...
Source: Protein and Cell - April 23, 2021 Category: Cytology Authors: Guang Lei Chao Mao Yuelong Yan Li Zhuang Boyi Gan Source Type: research

Molecular basis and homeostatic regulation of Zinc taste
Protein Cell. 2021 Apr 23. doi: 10.1007/s13238-021-00845-8. Online ahead of print.NO ABSTRACTPMID:33891304 | DOI:10.1007/s13238-021-00845-8 (Source: Protein and Cell)
Source: Protein and Cell - April 23, 2021 Category: Cytology Authors: Rui Luo Yuxiang Zhang Yinjun Jia Yan Zhang Zongyang Li Jieqing Zhao Ting Liu Wei Zhang Source Type: research

Ferroptosis, radiotherapy, and combination therapeutic strategies
Protein Cell. 2021 Apr 23. doi: 10.1007/s13238-021-00841-y. Online ahead of print.ABSTRACTFerroptosis, an iron-dependent form of regulated cell death driven by peroxidative damages of polyunsaturated-fatty-acid-containing phospholipids in cellular membranes, has recently been revealed to play an important role in radiotherapy-induced cell death and tumor suppression, and to mediate the synergy between radiotherapy and immunotherapy. In this review, we summarize known as well as putative mechanisms underlying the crosstalk between radiotherapy and ferroptosis, discuss the interactions between ferroptosis and other forms of ...
Source: Protein and Cell - April 23, 2021 Category: Cytology Authors: Guang Lei Chao Mao Yuelong Yan Li Zhuang Boyi Gan Source Type: research

Molecular basis and homeostatic regulation of Zinc taste
Protein Cell. 2021 Apr 23. doi: 10.1007/s13238-021-00845-8. Online ahead of print.NO ABSTRACTPMID:33891304 | DOI:10.1007/s13238-021-00845-8 (Source: Protein and Cell)
Source: Protein and Cell - April 23, 2021 Category: Cytology Authors: Rui Luo Yuxiang Zhang Yinjun Jia Yan Zhang Zongyang Li Jieqing Zhao Ting Liu Wei Zhang Source Type: research

Isogenic human pluripotent stem cell disease models reveal ABRA deficiency underlies cTnT mutation-induced familial dilated cardiomyopathy
Protein Cell. 2021 Apr 22. doi: 10.1007/s13238-021-00843-w. Online ahead of print.NO ABSTRACTPMID:33884582 | DOI:10.1007/s13238-021-00843-w (Source: Protein and Cell)
Source: Protein and Cell - April 22, 2021 Category: Cytology Authors: Bin Li Yongkun Zhan Qianqian Liang Chen Xu Xinyan Zhou Huanhuan Cai Yufan Zheng Yifan Guo Lei Wang Wenqing Qiu Baiping Cui Chao Lu Ruizhe Qian Ping Zhou Haiyan Chen Yun Liu Sifeng Chen Xiaobo Li Ning Sun Source Type: research

Nuclear m < sup > 6 < /sup > A reader YTHDC1 regulates the scaffold function of LINE1 RNA in mouse ESCs and early embryos
Protein Cell. 2021 Apr 22. doi: 10.1007/s13238-021-00837-8. Online ahead of print.ABSTRACTN6-methyladenosine (m6A) on chromosome-associated regulatory RNAs (carRNAs), including repeat RNAs, plays important roles in tuning the chromatin state and transcription, but the intrinsic mechanism remains unclear. Here, we report that YTHDC1 plays indispensable roles in the self-renewal and differentiation potency of mouse embryonic stem cells (ESCs), which highly depends on the m6A-binding ability. Ythdc1 is required for sufficient rRNA synthesis and repression of the 2-cell (2C) transcriptional program in ESCs, which recapitulates...
Source: Protein and Cell - April 22, 2021 Category: Cytology Authors: Chuan Chen Wenqiang Liu Jiayin Guo Yuanyuan Liu Xuelian Liu Jun Liu Xiaoyang Dou Rongrong Le Yixin Huang Chong Li Lingyue Yang Xiaochen Kou Yanhong Zhao You Wu Jiayu Chen Hong Wang Bin Shen Yawei Gao Shaorong Gao Source Type: research

Isogenic human pluripotent stem cell disease models reveal ABRA deficiency underlies cTnT mutation-induced familial dilated cardiomyopathy
Protein Cell. 2021 Apr 22. doi: 10.1007/s13238-021-00843-w. Online ahead of print.NO ABSTRACTPMID:33884582 | DOI:10.1007/s13238-021-00843-w (Source: Protein and Cell)
Source: Protein and Cell - April 22, 2021 Category: Cytology Authors: Bin Li Yongkun Zhan Qianqian Liang Chen Xu Xinyan Zhou Huanhuan Cai Yufan Zheng Yifan Guo Lei Wang Wenqing Qiu Baiping Cui Chao Lu Ruizhe Qian Ping Zhou Haiyan Chen Yun Liu Sifeng Chen Xiaobo Li Ning Sun Source Type: research

Nuclear m < sup > 6 < /sup > A reader YTHDC1 regulates the scaffold function of LINE1 RNA in mouse ESCs and early embryos
Protein Cell. 2021 Apr 22. doi: 10.1007/s13238-021-00837-8. Online ahead of print.ABSTRACTN6-methyladenosine (m6A) on chromosome-associated regulatory RNAs (carRNAs), including repeat RNAs, plays important roles in tuning the chromatin state and transcription, but the intrinsic mechanism remains unclear. Here, we report that YTHDC1 plays indispensable roles in the self-renewal and differentiation potency of mouse embryonic stem cells (ESCs), which highly depends on the m6A-binding ability. Ythdc1 is required for sufficient rRNA synthesis and repression of the 2-cell (2C) transcriptional program in ESCs, which recapitulates...
Source: Protein and Cell - April 22, 2021 Category: Cytology Authors: Chuan Chen Wenqiang Liu Jiayin Guo Yuanyuan Liu Xuelian Liu Jun Liu Xiaoyang Dou Rongrong Le Yixin Huang Chong Li Lingyue Yang Xiaochen Kou Yanhong Zhao You Wu Jiayu Chen Hong Wang Bin Shen Yawei Gao Shaorong Gao Source Type: research

Sarm1-mediated neurodegeneration within the enteric nervous system protects against local inflammation of the colon
Protein Cell. 2021 Apr 19. doi: 10.1007/s13238-021-00835-w. Online ahead of print.ABSTRACTAxonal degeneration is one of the key features of neurodegenerative disorders. In the canonical view, axonal degeneration destructs neural connections and promotes detrimental disease defects. Here, we assessed the enteric nervous system (ENS) of the mouse, non-human primate, and human by advanced 3D imaging. We observed the profound neurodegeneration of catecholaminergic axons in human colons with ulcerative colitis, and similarly, in mouse colons during acute dextran sulfate sodium-induced colitis. However, we unexpectedly revealed ...
Source: Protein and Cell - April 19, 2021 Category: Cytology Authors: Yue Sun Qi Wang Yi Wang Wenran Ren Ying Cao Jiali Li Xin Zhou Wei Fu Jing Yang Source Type: research

High-throughput screening identifies established drugs as SARS-CoV-2 PLpro inhibitors
In this study, we screened over 6,000 compounds that included approved drugs, drug candidates in clinical trials, and pharmacologically active compounds to identify leads that target the SARS-CoV-2 papain-like protease (PLpro). Together with main protease (Mpro), PLpro is responsible for processing the viral replicase polyprotein into functional units. Therefore, it is an attractive target for antiviral drug development. Here we discovered four compounds, YM155, cryptotanshinone, tanshinone I and GRL0617 that inhibit SARS-CoV-2 PLpro with IC50 values ranging from 1.39 to 5.63 μmol/L. These compounds also exhibit strong ...
Source: Protein and Cell - April 17, 2021 Category: Cytology Authors: Yao Zhao Xiaoyu Du Yinkai Duan Xiaoyan Pan Yifang Sun Tian You Lin Han Zhenming Jin Weijuan Shang Jing Yu Hangtian Guo Qianying Liu Yan Wu Chao Peng Jun Wang Chenghao Zhu Xiuna Yang Kailin Yang Ying Lei Luke W Guddat Wenqing Xu Gengfu Xiao Lei Sun Leike Z Source Type: research

Higher-order assemblies in immune signaling: supramolecular complexes and phase separation
Protein Cell. 2021 Apr 9. doi: 10.1007/s13238-021-00839-6. Online ahead of print.ABSTRACTSignaling pathways in innate and adaptive immunity play vital roles in pathogen recognition and the functions of immune cells. Higher-order assemblies have recently emerged as a central principle that governs immune signaling and, by extension, cellular communication in general. There are mainly two types of higher-order assemblies: 1) ordered, solid-like large supramolecular complexes formed by stable and rigid protein-protein interactions, and 2) liquid-like phase-separated condensates formed by weaker and more dynamic intermolecular...
Source: Protein and Cell - April 9, 2021 Category: Cytology Authors: Shiyu Xia Zhenhang Chen Chen Shen Tian-Min Fu Source Type: research

The hypothalamus for whole-body physiology: from metabolism to aging
Protein Cell. 2021 Apr 7. doi: 10.1007/s13238-021-00834-x. Online ahead of print.ABSTRACTObesity and aging are two important epidemic factors for metabolic syndrome and many other health issues, which contribute to devastating diseases such as cardiovascular diseases, stroke and cancers. The brain plays a central role in controlling metabolic physiology in that it integrates information from other metabolic organs, sends regulatory projections and orchestrates the whole-body function. Emerging studies suggest that brain dysfunction in sensing various internal cues or processing external cues may have profound effects on me...
Source: Protein and Cell - April 7, 2021 Category: Cytology Authors: Tiemin Liu Yong Xu Chun-Xia Yi Qingchun Tong Dongsheng Cai Source Type: research

Indiscriminate ssDNA cleavage activity of CRISPR-Cas12a induces no detectable off-target effects in mouse embryos
Protein Cell. 2021 Apr 1. doi: 10.1007/s13238-021-00824-z. Online ahead of print.NO ABSTRACTPMID:33797032 | DOI:10.1007/s13238-021-00824-z (Source: Protein and Cell)
Source: Protein and Cell - April 2, 2021 Category: Cytology Authors: Yu Wei Yingsi Zhou Yajing Liu Wenqin Ying Ruiming Lv Qimeng Zhao Haibo Zhou Erwei Zuo Yidi Sun Hui Yang Changyang Zhou Source Type: research

Understanding the phase separation characteristics of nucleocapsid protein provides a new therapeutic opportunity against SARS-CoV-2
Protein Cell. 2021 Mar 26:1-7. doi: 10.1007/s13238-021-00832-z. Online ahead of print.NO ABSTRACTPMID:33770364 | PMC:PMC7994959 | DOI:10.1007/s13238-021-00832-z (Source: Protein and Cell)
Source: Protein and Cell - March 26, 2021 Category: Cytology Authors: Dan Zhao Weifan Xu Xiaofan Zhang Xiaoting Wang Yiyue Ge Enming Yuan Yuanpeng Xiong Shenyang Wu Shuya Li Nian Wu Tingzhong Tian Xiaolong Feng Hantao Shu Peng Lang Jingxin Li Fengcai Zhu Xiaokun Shen Haitao Li Pilong Li Jianyang Zeng Source Type: research

Understanding the phase separation characteristics of nucleocapsid protein provides a new therapeutic opportunity against SARS-CoV-2
Protein Cell. 2021 Mar 26. doi: 10.1007/s13238-021-00832-z. Online ahead of print.NO ABSTRACTPMID:33770364 | DOI:10.1007/s13238-021-00832-z (Source: Protein and Cell)
Source: Protein and Cell - March 26, 2021 Category: Cytology Authors: Dan Zhao Weifan Xu Xiaofan Zhang Xiaoting Wang Yiyue Ge Enming Yuan Yuanpeng Xiong Shenyang Wu Shuya Li Nian Wu Tingzhong Tian Xiaolong Feng Hantao Shu Peng Lang Jingxin Li Fengcai Zhu Xiaokun Shen Haitao Li Pilong Li Jianyang Zeng Source Type: research

Islet organoid as a promising model for diabetes
Protein Cell. 2021 Mar 10:1-19. doi: 10.1007/s13238-021-00831-0. Online ahead of print.ABSTRACTStudies on diabetes have long been hampered by a lack of authentic disease models that, ideally, should be unlimited and able to recapitulate the abnormalities involved in the development, structure, and function of human pancreatic islets under pathological conditions. Stem cell-based islet organoids faithfully recapitulate islet development in vitro and provide large amounts of three-dimensional functional islet biomimetic materials with a morphological structure and cellular composition similar to those of native islets. Thus,...
Source: Protein and Cell - March 22, 2021 Category: Cytology Authors: Xiaofei Zhang Zhuo Ma Eli Song Tao Xu Source Type: research