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Specialty: Reproduction Medicine

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Total 316 results found since Jan 2013.

Periostin Enhances Migration, Invasion, and Adhesion of Human Endometrial Stromal Cells Through Integrin-Linked Kinase 1/Akt Signaling Pathway
Although our previous study confirmed that periostin (PN) was overexpressed in the eutopic and ectopic endometrial stroma of women with endometriosis by immunohistochemitry, the role of PN in the pathophysiology of endometriosis remains unknown. Thus, we aimed to investigate the effects of PN on endometrial stromal cells (ESCs) migration, invasion, adhesion, and proliferation and to further study the mechanism under this process. Eutopic (EuSCs), ectopic (EcSCs), and normal ESCs (NSCs) were isolated and cultured. We evaluated the above-mentioned biology behaviors and the expression of PN, integrin-linked kinase 1 (ILK1), a...
Source: Reproductive Sciences - August 10, 2015 Category: Reproduction Medicine Authors: Xu, X., Zheng, Q., Zhang, Z., Zhang, X., Liu, R., Liu, P. Tags: Original Articles Source Type: research

Role of PAFAH1B1 in human spermatogenesis, fertilization and early embryonic development
Publication date: Available online 7 August 2015 Source:Reproductive BioMedicine Online Author(s): Gui-Dong Yao, Sen-Lin Shi, Wen-Yan Song, Hai-Xia Jin, Zhao-Feng Peng, Hong-Yi Yang, En-Yin Wang, Ying-Pu Sun Spermatogenesis, fertilization and subsequent embryonic development are complex processes that require tight regulation. The PAFAH1B1 gene plays important roles in these reproductive events in mice, but its expression and roles in human reproduction have not been investigated. Expression analysis of testicular tissue by reverse transcription quantitative PCR and immunohistochemistry revealed varied expression...
Source: Reproductive BioMedicine Online - August 8, 2015 Category: Reproduction Medicine Source Type: research

Progestin and adipoQ Receptor 7, Progesterone Membrane Receptor Component 1 (PGRMC1) and PGRMC2 and Their Role in Regulating Progesterone's Ability to Suppress Human Granulosa/Luteal Cells from Entering into the Cell Cycle.
Abstract The present studies were designed to determine the role of Progesterone Receptor Membrane Component 1 (PGRMC1), PGRMC2, Progestin and AdipoQ Receptor 7 (PAQR7) and Progesterone Receptor (PGR) in mediating the anti-mitotic action of progesterone (P4) in human granulosa/luteal cells. For these studies granulosa/luteal cells of ten women undergoing controlled ovarian hyperstimulation were isolated, maintained in culture, and depleted of PGRMC1, PGRMC2, PAQR7 or PGR by siRNA treatment. The rate of entry into the cell cycle was assessed using the FUCCI cell cycle sensor to determine the percentage of cells in ...
Source: Biology of Reproduction - July 22, 2015 Category: Reproduction Medicine Authors: Sueldo C, Liu X, Peluso JJ Tags: Biol Reprod Source Type: research

Inhibition of DDAH1, but not DDAH2, results in apoptosis of a human trophoblast cell line in response to TRAIL
This study used the human extravillous trophoblast-derived cell line SGHPL-4 cells. All experiments were performed at least three times. PARTICIPANTS/MATERIALS, SETTING, METHODS The effect of DDAH on trophoblast apoptosis was examined using siRNA and time-lapse microscopy. Changes in the expression of DDAH were followed by PCR and western blot analysis. Receptor expression was followed by flow cytometry. MAIN RESULTS AND THE ROLE OF CHANCE Inhibiting the expression of DDAH1, but not DDAH2, resulted in a significant increase in the sensitivity of the EVT cell line SGHPL-4 to tumour necrosis factor related apoptosis induci...
Source: Human Reproduction - July 19, 2015 Category: Reproduction Medicine Authors: Lumicisi, B. A., Cartwright, J. E., Leslie, K., Wallace, A. E., Whitley, G. S. Tags: Early pregnancy Source Type: research

New insights into the function of Cullin 3 in trophoblast invasion and migration
In this study, immunostaining revealed that CUL3 was strongly expressed in the villous cytotrophoblasts, the trophoblast column, and the invasive extravillous trophoblasts. Silencing CUL3 significantly inhibited the outgrowth of villous explant ex vivo and decreased invasion and migration of trophoblast HTR8/SVneo cells. Furthermore, CUL3 siRNA decreased pro-MMP9 activity and increased the levels of TIMP1 and 2. We also found that the level of CUL3 in the placental villi from pre-eclamptic patients was significantly lower as compared to that from their gestational age-matched controls. Moreover, in the lentiviral-mediated ...
Source: Reproduction - July 9, 2015 Category: Reproduction Medicine Authors: Zhang, Q., Yu, S., Huang, X., Tan, Y., Zhu, C., Wang, Y.-L., Wang, H., Lin, H.-Y., Fu, J., Wang, H. Tags: Research Source Type: research

Mobilization of Dormant Cnot7 mRNA Promotes Deadenylation of Maternal Transcripts During Mouse Oocyte Maturation.
We report here that Cnot7, Cnot6l, and Pan2, key components of deadenylation machinery are also dormant maternal mRNAs that are recruited during oocyte maturation. Inhibiting the maturation-associated increase in CNOT7 (or CNOT6L) using an siRNA approach inhibits mRNA deadenylation, whereas inhibiting the increase in PAN2 has little effect. Reciprocally, expressing CNOT7 (or CNOT6L) in oocytes prevented from resuming meiosis initiates deadenylation of mRNAs. These effects on deadenylation are also observed when the total amount of poly(A) is quantified. Last, inhibiting the increase in CNOT7 protein results in an ~70% decr...
Source: Biology of Reproduction - July 1, 2015 Category: Reproduction Medicine Authors: Ma J, Fukuda Y, Schultz RM Tags: Biol Reprod Source Type: research

The Role of Endometrial Selectins and Their Ligands on Bovine Conceptus Attachment to the Uterine Epithelium During Peri-Implantation Period.
The objectives of this study were to determine if selectins and their ligands were expressed in the bovine conceptus and/or uterus during the peri-attachment period, and to study whether selectins were associated with conceptus attachment to the uterine epithelium. Through the RNA-seq analysis of bovine conceptuses on Days 17, 20, and 22 (Day 0 = day of estrus), only the L-selectin (SELL) ligand, podocalyxin (PODXL) and P-selectin (SELP) ligand, SELPLG were found. Quantitative PCR analysis confirmed the presence of PODXL and SELPLG in these conceptuses and revealed that SELL, mRNA and protein, detected in the uterine epith...
Source: Biology of Reproduction - July 1, 2015 Category: Reproduction Medicine Authors: Bai R, Kusama K, Sakurai T, Bai H, Wang C, Zhang J, Kuse M, Ideta A, Aoyagi Y, Okuda K, Imakawa K Tags: Biol Reprod Source Type: research

NEDD8-mediated neddylation is required for human endometrial stromal proliferation and decidualization
STUDY QUESTION Does NEDD8-mediated neddylation regulate human endometrial stromal proliferation and decidualization? SUMMARY ANSWER Neddylation inhibition by a selective NEDD8-activating enzyme inhibitor, MLN4924, significantly impairs human endometrial stromal cell (HESC) proliferation and decidualization and facilitates cell senescence, via p21 accumulation. WHAT IS KNOWN ALREADY Neddylation regulates cell proliferation and tissue remodeling during embryogenesis and tumorigenesis, while human endometrial stroma undergoes sequential proliferation, differentiation, as well as dynamic tissue remodeling during each menstru...
Source: Human Reproduction - June 18, 2015 Category: Reproduction Medicine Authors: Liao, Y., Jiang, Y., He, H., Ni, H., Tu, Z., Zhang, S., Wang, B., Lou, J., Quan, S., Wang, H. Tags: Reproductive biology Source Type: research

Novel Targets for the Transcription Factors MEF2 in MA-10 Leydig Cells.
Abstract Testosterone production by Leydig cells is a tightly regulated process requiring synchronized expression of several steroidogenic genes by numerous transcription factors. Myocyte enhancer factor 2 (MEF2) are transcription factors recently identified in somatic cells of the male gonad. In other tissues, MEF2 factors are essential regulators of organogenesis and cell differentiation. So far in the testis, MEF2 factors were found to regulate Leydig cell steroidogenesis by controlling Nr4a1 and Star gene expression. To expand our understanding of the role of MEF2 in Leydig cells, we performed microarray analy...
Source: Biology of Reproduction - May 27, 2015 Category: Reproduction Medicine Authors: Di-Luoffo M, Daems C, Bergeron F, Tremblay JJ Tags: Biol Reprod Source Type: research

Rhox8 Ablation in the Sertoli Cells Using a Tissue-Specific RNAi Approach Results in Impaired Male Fertility in Mice.
Abstract The reproductive homeobox X-linked, Rhox, genes encode transcription factors that are selectively expressed in reproductive tissues. While there are 33 Rhox genes in mice, only Rhox5 and Rhox8 are expressed in Sertoli cells, suggesting that they may regulate the expression of somatic-cell gene products crucial for germ-cell development. We previously characterized Rhox5-null mice, which are subfertile, exhibiting excessive germ-cell apoptosis and compromised sperm motility. To assess the role of Rhox8 in Sertoli cells, we used a tissue-specific RNAi approach to knockdown RHOX8 in vivo, in which the Rhox5 ...
Source: Biology of Reproduction - May 13, 2015 Category: Reproduction Medicine Authors: Welborn JP, Davis MG, Ebers SD, Stodden GR, Hayashi K, Cheatwood JC, Rao MK, MacLean JA Tags: Biol Reprod Source Type: research

Sohlh2 inhibits the apoptosis of mouse primordial follicle oocytes via C-kit/PI3K/Akt/Foxo3a signalling pathway
Publication date: May 2015 Source:Reproductive BioMedicine Online, Volume 30, Issue 5 Author(s): Xiaoli Zhang , Haiyu Zhang , Qing Gao , Shufang Ji , Lujun Bing , Jing Hao We previously reported that bone morphogenetic protein 4/drosophila mothers against decapentaplegic protein (BMP4/Smad) signalling pathway initiated primordial follicle growth and prevented oocyte apoptosis via up-regulation of Sohlh2 and receptor for kit ligand (c-kit). The mechanism underlying this process was not fully elucidated. In the present study, primary oocyte cultures were established from ovaries of 3-day-old female mouse pups by two-step e...
Source: Reproductive BioMedicine Online - May 2, 2015 Category: Reproduction Medicine Source Type: research

Potential effects of interferon regulatory factor 4 in a murine model of polyinosinic-polycytidylic acid-induced embryo resorption.
Abstract Interferon regulatory factor (IRF) 4 has been reported to modulate Toll-like receptor (TLR) signalling. Polyinosinic-polycytidylic acid (poly(I:C)) can be specifically recognised by TLR3, triggering the innate immune response and subsequently resulting in pregnancy loss. In the present study, poly(I:C) was administered to mice with or without TLR3 blockade. Chemokine (C-X-C motif) receptor 4 (CXCR4) expression was measured with or without chemokine (C-X-C motif) ligand 12 (CXCL12) inhibition. In cultured murine splenic mononuclear cells, IRF4 was knocked down by a specific short interference (si) RNA. IRF...
Source: Reproduction, Fertility, and Development - April 15, 2015 Category: Reproduction Medicine Authors: Wang J, Yin T, Wen Y, Tian F, He X, Zhou D, Lin Y, Yang J Tags: Reprod Fertil Dev Source Type: research

Decreased STAT3 in human idiopathic fetal growth restriction contributes to trophoblast dysfunction
In conclusion, we report that decreased STAT3 expression in placentae may contribute to abnormal trophoblast function in idiopathic FGR-affected pregnancies.
Source: Reproduction - March 30, 2015 Category: Reproduction Medicine Authors: Borg, A. J., Yong, H. E. J., Lappas, M., Degrelle, S. A., Keogh, R. J., Da Silva-Costa, F., Fournier, T., Abumaree, M., Keelan, J. A., Kalionis, B., Murthi, P. Tags: Research Source Type: research

KLF5 regulates infection- and inflammation-induced pro-labour mediators in human myometrium
In conclusion, this study describes novel data in which KLF5 is increased in labouring myometrium, and KLF5 silencing decreased inflammation- and infection-induced pro-labour mediators.
Source: Reproduction - March 27, 2015 Category: Reproduction Medicine Authors: Lappas, M. Tags: Research Source Type: research

Gonadotropin-Releasing Hormone Type II (GnRH-II) Agonist Regulates the Motility of Human Decidual Endometrial Stromal Cells: Possible Effect on Embryo Implantation and Pregnancy.
Abstract Invasion of the maternal decidua by extravillous trophoblast is an important process for embryo implantation and placentation in human. Motile behavior of decidual endometrial stromal cells have been considered of critical importance for embryo implantation and programming of human pregnancy. The GnRH effects in endometrium have raised concerns in reproduction. In the present study, we examined the action of GnRH-II agonist-promoted motility of human decidual endometrial stromal cells and the mechanisms of the action, indicating the role of GnRH-II agonist in embryo implantation and early pregnancy. Human...
Source: Biology of Reproduction - March 11, 2015 Category: Reproduction Medicine Authors: Wu HM, Huang HY, Lee CL, Soong YK, Leung PC, Wang HS Tags: Biol Reprod Source Type: research