Chromoanasynthesis: another way for the formation of complex chromosomal abnormalities in human reproduction
AbstractChromoanasynthesis has been described as a novel cause of massive constitutional chromosomal rearrangements. Based on DNA replication machinery defects, chromoanasynthesis is characterized by the presence of chromosomal duplications and triplications locally clustered on one single chromosome, or a few chromosomes, associated with various other types of structural rearrangements. Two distinct mechanisms have been described for the formation of these chaotic genomic disorders, i.e. the fork stalling and template switching and the microhomology-mediated break-induced replication. Micronucleus-based processes have bee...
Source: Human Reproduction - July 25, 2018 Category: Reproduction Medicine Source Type: research

Asherman ’s Syndrome: it may not be all our fault
AbstractAsherman ’s Syndrome (AS) is an acquired condition defined by the presence of intrauterine adhesions (IUA) that cause symptoms such as menstrual abnormalities, pelvic pain, infertility, recurrent miscarriage, abnormal placentation and attendant psychological distress. Classically, AS is considered an iatro genic disease triggered by trauma to the pregnant uterus. Different factors can cause the destruction of the endometrium, thus affecting the endometrial stem cell niche and creating IUAs. Curettage of the pregnant uterus appears to be the most common source of this destruction. Nevertheless, some AS cases h...
Source: Human Reproduction - July 25, 2018 Category: Reproduction Medicine Source Type: research

Editor ’s Choice: Fatigue and endometriosis
(Source: Human Reproduction)
Source: Human Reproduction - July 25, 2018 Category: Reproduction Medicine Source Type: research

Defining the limits of detection for chromosome rearrangements in the preimplantation embryo using next generation sequencing
AbstractSTUDY QUESTIONIs next generation sequencing (NGS) capable of detecting smaller sub-chromosomal rearrangements in human embryos than the manufacturer ’s quoted resolution suggests?SUMMARY ANSWERNGS was able to detect unbalanced chromosome segments smaller than the manufacturer ’s resolution.WHAT IS KNOWN ALREADYArray Comparative Genomic Hybridization (array-CGH) has been the gold standard platform used for PGD of chromosome rearrangements. NGS is a viable alternative to array-CGH for PGD of chromosome arrangements given that the manufacturer ’s guidelines quote a resolution of ≥20 Mb. However, a...
Source: Human Reproduction - July 10, 2018 Category: Reproduction Medicine Source Type: research

Reproduction after the loss of a child: a population-based matched cohort study
AbstractSTUDY QUESTIONIs the death of a child associated with higher subsequent fertility?SUMMARY ANSWERWomen who had lost a child had higher fertility both shortly after the loss and throughout the entire follow-up, independent of the child ’s age at the time of death.WHAT IS KNOWN ALREADYWomen who lose a child in the perinatal period often have another child shortly after. However, to our knowledge no previous study has investigated if the death of an older child affects reproductive behavior.STUDY DESIGN, SIZE, DURATIONThe source population for this matched cohort study consisted of all women who gave birth in Den...
Source: Human Reproduction - July 10, 2018 Category: Reproduction Medicine Source Type: research

Performance of prognostic modelling of high and low ovarian response to ovarian stimulation for IVF
AbstractSTUDY QUESTIONWhat is the performance of previously established regression models in predicting low and high ovarian response to 150 μg corifollitropin alfa/GnRH-antagonist ovarian stimulation in an independent dataset?SUMMARY ANSWERThe outcome of ovarian stimulation with 150 μg corifollitropin alfa in a fixed, multiple dose GnRH-antagonist protocol can be validly predicted using logistic regression models with AMH being of paramount importance.WHAT IS KNOWN ALREADYPredictors of ovarian response have been identified in FSH/GnRH agonist protocols as well as ovarian stimulation with corifollitropin alfa/GnRH-an...
Source: Human Reproduction - July 10, 2018 Category: Reproduction Medicine Source Type: research

Diminished ovarian reserve and poor response to stimulation in patients & lt;38 years old: a quantitative but not qualitative reduction in performance
AbstractSTUDY QUESTIONDo infertile women aged
Source: Human Reproduction - July 10, 2018 Category: Reproduction Medicine Source Type: research

Microfluidic sorting selects sperm for clinical use with reduced DNA damage compared to density gradient centrifugation with swim-up in split semen samples
AbstractSTUDY QUESTIONDoes microfluidic sorting improve the selection of sperm with lower DNA fragmentation over standard density-gradient centrifugation?SUMMARY ANSWERMicrofluidic sorting of unprocessed semen allows for the selection of clinically usable, highly motile sperm with nearly undetectable levels of DNA fragmentation.WHAT IS KNOWN ALREADYMicrofluidic devices have been explored to sort motile and morphologically normal sperm from a raw sample without centrifugation; however, it is uncertain whether DNA damage is reduced in this process.STUDY DESIGN, SIZE, DURATIONThis is a blinded, controlled laboratory study of ...
Source: Human Reproduction - July 10, 2018 Category: Reproduction Medicine Source Type: research

Corrigendum. REPLY: The ‘Big Freeze’: freeze-all should not be used for everyone
(Source: Human Reproduction)
Source: Human Reproduction - July 9, 2018 Category: Reproduction Medicine Source Type: research

Long-term effects of childhood cancer treatment on hormonal and ultrasound markers of ovarian reserve
This study demonstrated that the majority of CCSs do not show signs of a reduced ovarian reserve. However, specific subgroups of CCSs appear to be associated with a high risk. Our results are important for counselling CCSs and future patients regarding parenthood and fertility preservation.STUDY FUNDING/COMPETING INTERESTSThis study was funded by the Dutch Cancer Society (Grant no. VU 2006-3622) and by the Children Cancer Free Foundation (Project no. 20). Philips Health Systems Benelux supported this study by providing three ultrasound systems and concomitant analytic software. There are no competing interests.TRIAL REGIST...
Source: Human Reproduction - July 6, 2018 Category: Reproduction Medicine Source Type: research

Maternal and paternal preconception exposure to bisphenols and size at birth
This study suggests that the unexplored maternal preconception period may be a sensitive window for BPA effects on birth outcomes.STUDY FUNDING/COMPETING INTEREST(S)Work supported by Grants (ES R01 009718, ES 022955 and ES 000002) from the National Institute of Environmental Health Sciences (NIEHS). C.M. was supported by a post-doctoral fellowship award from the Canadian Institutes of Health Research. There are no competing interests to declare. (Source: Human Reproduction)
Source: Human Reproduction - July 4, 2018 Category: Reproduction Medicine Source Type: research

Assisted reproductive technology and the risk of unplanned peripartum hysterectomy: analysis using propensity score matching
AbstractSTUDY QUESTIONIs there an increased risk of unplanned peripartum hysterectomy in pregnancies with assissted reproductive technology compared to those without ART?SUMMARY ANSWERAlthough the absolute risks are low, there is an almost five-fold increased risk of unplanned peripartum hysterectomy and 1.7 more unplanned peripartum hysterectomies occur per 1000 deliveries in pregnancies with ART compared to those without ART.WHAT IS KNOWN ALREADYIt has been reported that pregnancies with ART was associated with increased risk of peripartum hysterectomy in one case –control study and in one cohort study.STUDY DESIGN...
Source: Human Reproduction - July 4, 2018 Category: Reproduction Medicine Source Type: research

Epididymal more than testicular abnormalities are associated with the occurrence of antisperm antibodies as evaluated by the MAR test
AbstractSTUDY QUESTIONIs there any association between mixed antiglobulin reaction (MAR) test positivity and clinical features or genital tract ultrasound (US) parameter values in males of infertile and fertile couples?STUDY ANSWERIn males of infertile and fertile couples MAR test positivity was associated with clinical and US features suggestive of chronic epididymal inflammation.WHAT IS KNOWN ALREADYMAR test positivity has been more often reported in males of infertile couples than in fertile men. A positive MAR test has been detected in men with a history of testicular or post-testicular damage. No previous study has re...
Source: Human Reproduction - July 4, 2018 Category: Reproduction Medicine Source Type: research

Severe maternal morbidity in women with high BMI in IVF and unassisted singleton pregnancies
AbstractSTUDY QUESTIONIs there a synergistic risk of severe maternal morbidity (SMM) in overweight/obese women who conceived by IVF compared to normal-weight women without IVF?SUMMARY ANSWERSMM was more common in IVF pregnancies, and among overweight/obese women, but we did not detect a synergistic effect of both factors.WHAT IS KNOWN ALREADYWhile much is known about the impact of overweight and obesity on success rates after IVF, there is less data on maternal health outcomes.STUDY DESIGN, SIZE, DURATIONThis is a population-based cohort study of 114 409 singleton pregnancies with conceptions dating from 11 January 2013 un...
Source: Human Reproduction - July 3, 2018 Category: Reproduction Medicine Source Type: research

Suppression of COUP-TFII upregulates angiogenin and promotes angiogenesis in endometriosis
AbstractSTUDY QUESTIONHow does hypoxia-mediated downregulation of chicken ovalbumin upstream promoter-transcription factor II (COUP-TFII) promote angiogenesis in endometriosis?SUMMARY ANSWERSuppression of COUP-TFII by hypoxia stimulates angiogenesis through induction of angiogenin (ANG).WHAT IS KNOWN ALREADYThe level of COUP-TFII is downregulated in endometriotic tissues, and downregulation of COUP-TFII contributes to the development of endometriosis.STUDY DESIGN, SIZE, DURATIONTwenty-seven patients of reproductive age with endometriosis were recruited in this study. Eutopic endometrial and ectopic endometriotic stromal ce...
Source: Human Reproduction - July 3, 2018 Category: Reproduction Medicine Source Type: research

The impact of luteal serum progesterone levels on live birth rates —a prospective study of 602 IVF/ICSI cycles
This study is the first to explore the possible upper and lower thresholds for luteal P4 following IVF treatment and fresh embryo transfer, and the optimal P4 ranges found in this study should be corroborated in future clinical trials. Furthermore, the P4 thresholds in this study only apply to fresh IVF cycles, using vaginal luteal phase support, as the optimal P4 level in cycles using intramuscular P4 may be different.WIDER IMPLICATIONS OF THE FINDINGSFuture studies are necessary to explore whether additional exogenous luteal P4 supplementation in the low P4 group could increase the chance of a live birth following fresh ...
Source: Human Reproduction - June 28, 2018 Category: Reproduction Medicine Source Type: research

Trophoblast plugs: impact on utero-placental haemodynamics and spiral artery remodelling
AbstractSTUDY QUESTIONHow does trophoblast plugging impact utero-placental haemodynamics?SUMMARY ANSWERPhysiological trophoblast plug structures are dense enough to restrict flow of oxygenated blood to the intervillous space (IVS) in the first trimester, and result in a shear stress environment upstream of the plugs that promotes spiral artery remodelling.WHAT IS KNOWN ALREADYTrophoblast plugging of the uterine spiral arteries is thought to be the dominant factor restricting the flow of oxygenated maternal blood to the placenta in the first trimester of pregnancy. However, the extent of plugging, the timing of plug break u...
Source: Human Reproduction - June 28, 2018 Category: Reproduction Medicine Source Type: research

Fatigue – a symptom in endometriosis
This study focused on the symptom fatigue in endometriosis. Relationships of variables were established by regression analysis and associations were quantified as odds ratios.MAIN RESULTS AND THE ROLE OF CHANCEFrequent fatigue was experienced by a majority of women diagnosed with endometriosis (50.7% versus 22.4% in control women,P
Source: Human Reproduction - June 26, 2018 Category: Reproduction Medicine Source Type: research

Maltreatment during childhood: a risk factor for the development of endometriosis?
AbstractSTUDY QUESTIONIs maltreatment during childhood (MC), e.g. sexual abuse, physical abuse, emotional abuse and neglect, associated with diagnosis of endometriosis?SUMMARY ANSWERChildhood sexual abuse, emotional abuse/neglect and inconsistency experiences were associated with the diagnosis of endometriosis while no such association was found for physical abuse/neglect and other forms of maltreatment.WHAT IS KNOWN ALREADYSymptoms of endometriosis such as chronic pelvic pain, fatigue and depression, are correlated with MC, as are immune reactions linked to endometriosis. These factors support a case for a potential role ...
Source: Human Reproduction - June 26, 2018 Category: Reproduction Medicine Source Type: research

Editor ’s Choice: Fertility after cancer, these are promising times
(Source: Human Reproduction)
Source: Human Reproduction - June 20, 2018 Category: Reproduction Medicine Source Type: research

REPLY: The ‘Big Freeze’: freeze-all should not be used for everyone
(Source: Human Reproduction)
Source: Human Reproduction - June 19, 2018 Category: Reproduction Medicine Source Type: research

Reproductive life in women with celiac disease; a nationwide, population-based matched cohort study
This study was funded by the Health Research Fund of Central Denmark Region and The Hede Nielsens Foundation, Denmark. The authors report no conflicts of interest in this work. (Source: Human Reproduction)
Source: Human Reproduction - June 15, 2018 Category: Reproduction Medicine Source Type: research

Luteal phase anovulatory follicles result in the production of competent oocytes: intra-patient paired case-control study comparing follicular versus luteal phase stimulations in the same ovarian cycle
AbstractSTUDY QUESTIONAre the mean numbers of blastocysts obtained from sibling cohorts of oocytes recruited after follicular phase and luteal phase stimulations (FPS and LPS) in the same ovarian cycle similar?SUMMARY ANSWERThe cohorts of oocytes obtained after LPS are larger than their paired-FPS-derived cohorts and show a comparable competence, thus resulting in a larger mean number of blastocysts.WHAT IS KNOWN ALREADYThree theories of follicle recruitment have been postulated to date: (i) the ‘continuous recruitment’ theory, (ii) the ‘single recruitment episode’ theory and (iii) the ‘wave&r...
Source: Human Reproduction - June 15, 2018 Category: Reproduction Medicine Source Type: research

Impact on testicular function of a single ablative activity of 3.7 GBq radioactive iodine for differentiated thyroid carcinoma
AbstractSTUDY QUESTIONWhat are the consequences of radioactive iodine (RAI) therapy for testicular function?SUMMARY ANSWERA single activity of 3.7 GBq RAI for differentiated thyroid carcinoma (DTC) treatment in young men transiently altered Sertoli cell function and induced sperm chromosomal abnormalities.WHAT IS KNOWN ALREADYFew studies, mainly retrospective, have reported the potential impacts of RAI on endocrine and exocrine testicular function.STUDY DESIGN, SIZE, DURATIONA longitudinal prospective multi-center study on testicular function performed in DTC patients before a single131I ablative activity of 3.7 GBq (V0) a...
Source: Human Reproduction - June 15, 2018 Category: Reproduction Medicine Source Type: research

Peroxiredoxin 6 is the primary antioxidant enzyme for the maintenance of viability and DNA integrity in human spermatozoa
AbstractSTUDY QUESTIONAre all components of the peroxiredoxins (PRDXs) system important to control the levels of reactive oxygen species (ROS) to maintain viability and DNA integrity in spermatozoa?SUMMARY ANSWERPRDX6 is the primary player of the PRDXs system for maintaining viability and DNA integrity in human spermatozoa.WHAT IS KNOWN ALREADYMammalian spermatozoa are sensitive to high levels of ROS and PRDXs are antioxidant enzymes proven to control the levels of ROS generated during sperm capacitation to avoid oxidative damage in the spermatozoon. Low amounts of PRDXs are associated with male infertility. The absence of...
Source: Human Reproduction - June 15, 2018 Category: Reproduction Medicine Source Type: research

The ‘Big Freeze’: freeze-all should not be used for everyone
(Source: Human Reproduction)
Source: Human Reproduction - June 15, 2018 Category: Reproduction Medicine Source Type: research

On the pasta theory of reproductive research
(Source: Human Reproduction)
Source: Human Reproduction - June 15, 2018 Category: Reproduction Medicine Source Type: research

The impact of cancer on subsequent chance of pregnancy: a population-based analysis
This study was funded by NHS Lothian Cancer and Leukaemia Endowments Fund. Part of this work was undertaken in the MRC Centre for Reproductive Health which is funded by the MRC Centre grant MR/N022556/1. RAA has participated in Advisory Boards and/or received speaker ’s fees from Beckman Coulter, IBSA, Merck and Roche Diagnostics. He has received research support from Roche Diagnostics, Ansh labs and Ferring. The other authors have no conflicts to declare. (Source: Human Reproduction)
Source: Human Reproduction - June 15, 2018 Category: Reproduction Medicine Source Type: research

Novel homozygous nonsense mutations in LHCGR lead to empty follicle syndrome and 46, XY disorder of sex development
AbstractEmpty follicle syndrome (EFS) is a disorder associated with female infertility and presents as a complete failure to retrieve oocytes during ART cycles despite normal follicle development and careful aspiration. To date, only two EFS cases have been reported with homozygous missense mutations in the luteinizing hormone/chorionic gonadotropin receptor (LHCGR) gene, and both cases showed normal estradiol (E2) production during ovulation induction. The molecular genetic mechanisms of EFS remain unknown. Herein, we report two novel homozygous inactivatingLHCGR mutations, c.736 C>T (p.Q246*) and c.846dupT (p.R283*), ...
Source: Human Reproduction - June 15, 2018 Category: Reproduction Medicine Source Type: research

ICSI does not increase the cumulative live birth rate in non-male factor infertility
AbstractSTUDY QUESTIONWhat is the cumulative live birth rate following ICSI cycles compared with IVF cycles for couples with non-male factor infertility?SUMMARY ANSWERICSI resulted in a similar cumulative live birth rate compared with IVF for couples with non-male factor infertility.WHAT IS KNOWN ALREADYThe ICSI procedure was developed for couples with male factor infertility. There has been an increased use of ICSI regardless of the cause of infertility. Cycle-based statistics show that there is no difference in pregnancy rates between ICSI and IVF in couples with non-male factor infertility. However, evidence indicates t...
Source: Human Reproduction - June 12, 2018 Category: Reproduction Medicine Source Type: research

Increased AIF-1-mediated TNF- α expression during implantation phase in IVF cycles with GnRH antagonist protocol
AbstractSTUDY QUESTIONIs allograft inflammatory factor-1 (AIF-1), a cytokine associated with inflammation and allograft rejection, aberrantly elevated inin vitro fertilization (IVF) cycles with gonadotropin-releasing hormone (GnRH) antagonist protocol with potential effects on endometrial receptivity?SUMMARY ANSWEROur findings indicated AIF-1 is increased in IVF cycles with GnRH antagonist protocol and mediates greater TNF- α expression during implantation phase, which may be unfavorable for embryo implantation.WHAT IS KNOWN ALREADYStudies have shown that GnRH antagonist protocol cycles have lower implantation and cl...
Source: Human Reproduction - June 12, 2018 Category: Reproduction Medicine Source Type: research

Latent genital tuberculosis adversely affects the ovarian reserve in infertile women
AbstractSTUDY QUESTIONWhat is the effect of latent genital tuberculosis (GTB) on ovarian reserve in infertile women?SUMMARY ANSWERWomen with latent GTB have lower ovarian reserves and yield lower numbers of oocytes in IVF.WHAT IS KNOWN ALREADYLimited evidence suggests that women with GTB may have a low ovarian reserve. Infertile women have a high incidence of latent GTB and treatment improves fertility outcomes.STUDY DESIGN, SIZE, DURATIONThis prospective study from February 2013 to January 2016 compared 431 infertile women diagnosed with latent GTB (Group I) to 453 infertile women without latent GTB (Group II).PARTICIPANT...
Source: Human Reproduction - June 12, 2018 Category: Reproduction Medicine Source Type: research

Premature ovarian insufficiency and early depletion of the ovarian reserve in the monogenic Mulibrey nanism disorder
This study was supported by the Finnish Foundation for Pediatric Research, Finska L äkaresällskapet, the Sigrid Jusélius Foundation and Helsinki University Hospital Research Funds. The authors declare no conflicts of interest.TRIAL REGISTRATION NUMBERNot applicable. (Source: Human Reproduction)
Source: Human Reproduction - June 1, 2018 Category: Reproduction Medicine Source Type: research

Mutation-specific effects in germline transmission of pathogenic mtDNA variants
AbstractSTUDY QUESTIONDoes germline selection (besides random genetic drift) play a role during the transmission of heteroplasmic pathogenic mitochondrial DNA (mtDNA) mutations in humans?SUMMARY ANSWERWe conclude that inheritance of mtDNA is mutation-specific and governed by a combination of random genetic drift and negative and/or positive selection.WHAT IS KNOWN ALREADYmtDNA inherits maternally through a genetic bottleneck, but the underlying mechanisms are largely unknown. Although random genetic drift is recognized as an important mechanism, selection mechanisms are thought to play a role as well.STUDY DESIGN, SIZE, DU...
Source: Human Reproduction - May 30, 2018 Category: Reproduction Medicine Source Type: research

Testicular microbiome in azoospermic men —first evidence of the impact of an altered microenvironment
AbstractSTUDY QUESTIONGiven the relevant role of the extracellular microenvironment in regulating tissue homeostasis, is testicular bacterial microbiome (BM) associated with germ cell aplasia in idiopathic non-obstructive azoospermia (iNOA)?SUMMARY ANSWERA steady increase of dysbiosis was observed among testis with normal spermatogenesis vs. iNOA with positive sperm retrieval and iNOA with complete germ cell aplasia.WHAT IS KNOWN ALREADYTissue-associated BM has been reported to be a biologically important extracellular microenvironment component for numerous body habitats, but not yet for the human testis.STUDY DESIGN, SIZ...
Source: Human Reproduction - May 30, 2018 Category: Reproduction Medicine Source Type: research

Editor ’s Choice: Klinefelter patients lose germ cells earlier than we thought
(Source: Human Reproduction)
Source: Human Reproduction - May 24, 2018 Category: Reproduction Medicine Source Type: research

Chromosomal mosaicism in human blastocysts: the ultimate challenge of preimplantation genetic testing?
AbstractSTUDY QUESTIONTo what extent does a trophectoderm (TE) biopsy reliably reflect the chromosomal constitution of the inner cell mass (ICM) in human blastocysts?SUMMARY ANSWERConcordance between TE and ICM was established in 62.1% of the embryos analysed.WHAT IS KNOWN ALREADYNext generation sequencing (NGS) platforms have recently been optimised for preimplantation genetic testing for aneuploidies (PGT-A). However, higher sensitivity has led to an increase in reports of chromosomal mosaicism within a single TE biopsy. This has raised substantial controversy surrounding the prevalence of mosaicism in human blastocysts ...
Source: Human Reproduction - May 23, 2018 Category: Reproduction Medicine Source Type: research

Anxiety and depressive symptoms, and stress biomarkers in pregnant women after in vitro fertilization: a prospective cohort study
This study was performed in the Division of Obstetrics in a regional referral center. The State scale of the State-Trait Anxiety Inventory (STAI-S) and the Beck Depression Inventory-Sort Form (BDI/SF) were used as anxiety and depression indicators, respectively; salivary cortisol and α-amylase levels as stress biomarkers. Anxiety, depression and stress biomarkers were measured at the third trimester of pregnancy (T1), at 48 h after birth (T2) and at 3 months after birth (T3). Associations with IVF were assessed using ordinal mixed models for anxiety and depressive symptoms and linear quantile models for stress biomar...
Source: Human Reproduction - May 23, 2018 Category: Reproduction Medicine Source Type: research

Prevalence of XXY karyotypes in human blastocysts: multicentre data from 7549 trophectoderm biopsies obtained during preimplantation genetic testing cycles in IVF
AbstractSTUDY QUESTIONWhich is the prevalence of a 47,XXY karyotype in human blastocysts biopsied during preimplantation genetic testing for aneuploidies (PGT-A) cycles?SUMMARY ANSWERThe prevalence of a 47,XXY karyotype amongst male blastocysts without autosomal aneuploides is ~1%.WHAT IS KNOWN ALREADYThe prevalence of Klinefelter syndrome is estimated as 0.1 –0.2% in male newborns. However, the KS phenotype is extremely variable and there are men with a 47,XXY karyotype and less evident signs, who may go undetected. No risk factor for the 47,XXY karyotype in products of conception has been yet clearly defined, and n...
Source: Human Reproduction - May 19, 2018 Category: Reproduction Medicine Source Type: research

Reply: An informed decision between cleavage-stage and blastocyst-stage transfers in IVF requires data on the transfers of frozen –thawed embryos
(Source: Human Reproduction)
Source: Human Reproduction - May 18, 2018 Category: Reproduction Medicine Source Type: research

An informed decision between cleavage-stage and blastocyst-stage transfer in IVF requires data on the transfers of frozen –thawed embryos
(Source: Human Reproduction)
Source: Human Reproduction - May 18, 2018 Category: Reproduction Medicine Source Type: research

An experimental evaluation of the benefits and costs of providing fertility information to adolescents and emerging adults
AbstractSTUDY QUESTIONDoes the provision of fertility (compared to control) information affect fertility-related knowledge, perceived threat of infertility, anxiety, physical stress and fertility plans in adolescents and emerging adults?SUMMARY ANSWERThe provision of fertility information was associated with increased fertility knowledge (emerging adults) and greater infertility threat (adolescents and emerging adults).WHAT IS KNOWN ALREADYAccording to fertility education research, adolescents and emerging adults know less than they should know about fertility topics. Fertility knowledge can be improved through the provisi...
Source: Human Reproduction - May 17, 2018 Category: Reproduction Medicine Source Type: research

Pregnancy after vasectomy: surgical reversal or assisted reproduction?
This study was a retrospective single-center cohort analysis of all males with a vasectomy in the past seeking treatment between 2006 and 2011 (n = 163). One group of patients opted for a reanastomosis procedure while the others opted for an immediate IVF/ICSI treatment. This included 99 males who underwent reanastomosis and 64 couples who immediately underwent ICSI treatment.PARTICIPANTS/MATERIALS, SETTING, METHODSAll reanastomosis procedures were done by the same surgeon. ICSI was used in all cases where testicular sperm were extracted by fine needle aspiration (FNA) or testicular sperm extraction (TESE).MAIN RESULTS AND...
Source: Human Reproduction - May 17, 2018 Category: Reproduction Medicine Source Type: research

Bowel surgery as a fertility-enhancing procedure in patients with colorectal endometriosis: methodological, pathogenic and ethical issues
AbstractBowel surgery for colorectal endometriosis is being promoted to infertile women without severe sub-occlusive symptoms, with the objective of improving the likelihood of conception. Contrary to rectal shaving, bowel surgery involving full-thickness disk excision and segmental resection entails opening of the intestinal lumen thus increasing the risk of postoperative infectious complications. About 1 in 10 patients undergoing colorectal resection for intestinal endometriosis will experience severe sequelae, including anastomotic dehiscence, rectovaginal fistula formation, and bladder and bowel denervation. Similar to...
Source: Human Reproduction - May 7, 2018 Category: Reproduction Medicine Source Type: research

Pre-pregnancy fast food and fruit intake is associated with time to pregnancy
AbstractSTUDY QUESTIONIs preconception dietary intake associated with reduced fecundity as measured by a longer time to pregnancy (TTP)?SUMMARY ANSWERLower intake of fruit and higher intake of fast food in the preconception period were both associated with a longer TTP.WHAT IS KNOWN ALREADYSeveral lifestyle factors, such as smoking and obesity, have consistently been associated with a longer TTP or infertility, but the role of preconception diet in women remains poorly studied. Healthier foods or dietary patterns have been associated with improved fertility, however, these studies focused on women already diagnosed with or...
Source: Human Reproduction - May 4, 2018 Category: Reproduction Medicine Source Type: research

Defining quality assurance and quality control measures in connection with ovarian tissue cryopreservation and transplantation: a call to action
AbstractFreezing of ovarian tissue for fertility preservation has been gaining ground as a valid method in recent years. More than 100 children have been born from this procedure worldwide. As a result, many fertility clinics are now implementing this method. However, the practical procedures that need to be mastered to successfully implement the freezing of ovarian tissue are different in many aspects from those normally used in fertility clinics and are not well defined. Furthermore, success is difficult to measure since patients usually do not return for transplantation until several years after freezing, which puts ext...
Source: Human Reproduction - April 27, 2018 Category: Reproduction Medicine Source Type: research

Decreased clinical pregnancy and live birth rates after short interval from delivery to subsequent assisted reproductive treatment cycle
AbstractSTUDY QUESTIONDoes the interval from delivery to initiation of a subsequent ART treatment cycle impact clinical pregnancy or live birth rates?SUMMARY ANSWERAn interval from delivery to treatment start of
Source: Human Reproduction - April 27, 2018 Category: Reproduction Medicine Source Type: research

Corrigendum: Letter to the Editor: Individualized FSH dosing improves safety and reduces iatrogenic poor response while maintaining live-birth rates
(Source: Human Reproduction)
Source: Human Reproduction - April 27, 2018 Category: Reproduction Medicine Source Type: research

Novel mutations in PATL2 cause female infertility with oocyte germinal vesicle arrest
In this study, nine unrelated primary infertile females presenting with oocyte GV arrest were recruited during the treatment of early rescue ICSI or ICSI from January 2013 to December 2016.PARTICIPANTS/MATERIALS, SETTING, METHODSGenomic DNA was isolated from blood samples obtained from all nine affected individuals and all of their available family members. All the coding regions ofPATL2 were sequenced by Sanger sequencing. The pathogenicity of the identified variants and their possible effects on the protein were evaluatedin silico.MAIN RESULTS AND THE ROLE OF CHANCEFive novel point mutations and one recurrent splicing mu...
Source: Human Reproduction - April 25, 2018 Category: Reproduction Medicine Source Type: research

Single-cell analysis of [Ca2+]i signalling in sub-fertile men: characteristics and relation to fertilization outcome
This study reveals important details of impaired [Ca2+]i signalling in sperm from sub-fertile men that cannot be detected in population studies.STUDY FUNDING/COMPETING INTEREST(S)This study was funded by a MRC project grant (MR/M012492/1; MR/K013343/1). Additional funding was provided by Chief Scientist Office/NHS research Scotland. (Source: Human Reproduction)
Source: Human Reproduction - April 25, 2018 Category: Reproduction Medicine Source Type: research