International Committee for Monitoring Assisted Reproductive Technologies world report: assisted reproductive technology, 2014 †
This report covers approximately two-thirds of world ART activity. China is a major contributor of global cycles missing from this report. Continued efforts to improve the quality and consistency of ART data reported by registries are still needed, including the use of internationally agreed standard definitions (The International Glossary of Infertility and Fertility Care). A new method was introduced in this report to calculate ART utilization and number of babies born following ART; therefore, these results are not directly comparable with previous reports.WIDER IMPLICATIONS OF THE FINDINGSThe ICMART world reports provi...
Source: Human Reproduction - October 3, 2021 Category: Reproduction Medicine Source Type: research

What is the threshold of mature oocytes to obtain at least one healthy transferable cleavage-stage embryo after preimplantation genetic testing for fragile X syndrome?
This study is retrospective, analyzing a limited number of cycles. Moreover, the patients who were included in a fresh PGT cycle were selected on ovarian reserve parameters and show high values in ovarian reserve tests. This information could influence our conclusion.WIDER IMPLICATIONS OF THE FINDINGSThe results relate only to the target population of this study, with a correct ovarian reserve of AMH>1 and AFC>10. However, the information provided herein extends knowledge about the current state of COS forFMR1 mutation carriers in order to provide patients with proper counseling regarding the optimal number of oocyte...
Source: Human Reproduction - September 26, 2021 Category: Reproduction Medicine Source Type: research

Accelerated loss of oogonia and impaired folliculogenesis in females with Turner syndrome start during early fetal development
This study included a total of 102 formalin-fixed and paraffin-embedded fetal ovarian tissue specimens. Specimens included were from fetuses with 45,X (N  = 42 aged gestational week (GW) 12–20, except one GW 40 sample), 45,X/46,XX (N = 7, aged GW 12–20), and from controls (N = 53, aged GW 12–42) from a biobank (ethics approval # H-2-2014-103).PARTICIPANTS/MATERIALS, SETTING, METHODSThe number of OCT4 positive germ cells/mm2, follicles (primordial and primary)/mm2 and cPARP positive cells/mm2 were quantified in fetal ovarian tissue from TS, TS mosaic and controls following morphological and immunohistochem...
Source: Human Reproduction - September 26, 2021 Category: Reproduction Medicine Source Type: research

Liquid biopsy: state of reproductive medicine and beyond
ABSTRACTLiquid biopsy is the process of sampling and analyzing body fluids, which enables non-invasive monitoring of complex biological systemsin vivo. Liquid biopsy has myriad applications in health and disease as a wide variety of components, ranging from circulating cells to cell-free nucleic acid molecules, can be analyzed. Here, we review different components of liquid biopsy, survey state-of-the-art, non-invasive methods for detecting those components, demonstrate their clinical applications and discuss ethical considerations. Furthermore, we emphasize the importance of artificial intelligence in analyzing liquid bio...
Source: Human Reproduction - September 25, 2021 Category: Reproduction Medicine Source Type: research

Assessing couples ’ preferences for fresh or frozen embryo transfer: a discrete choice experiment
AbstractSTUDY QUESTIONWhat are couples ’ preferences for fresh embryo transfer versus freezing of all embryos followed by frozen embryo transfer and the associated clinical outcomes that may differentiate them?SUMMARY ANSWERCouples ’ preferences are driven by anticipated chances of live birth, miscarriage, neonatal complications, and costs but not by the differences in the treatment process (including delay of embryo transfer linked to frozen embryo transfer and risk of ovarian hyperstimulation syndrome (OHSS) associated wit h fresh embryo transfer).WHAT IS KNOWN ALREADYA policy of freezing all embryos followed by tran...
Source: Human Reproduction - September 22, 2021 Category: Reproduction Medicine Source Type: research

Mitochondria-related changes and metabolic dysfunction in low prognosis patients under the POSEIDON classification
This study was registered with the Chinese Clinical Trial Register (Clinical Trial Number: ChiCTR2100045531). (Source: Human Reproduction)
Source: Human Reproduction - September 21, 2021 Category: Reproduction Medicine Source Type: research

Optimisation of the timing of fertilisation assessment for oocytes cultured in standard incubation: lessons learnt from time-lapse imaging of 78 348 embryos
AbstractSTUDY QUESTIONUsing time-lapse data, can the current consensus for the timing of fertilisation assessment of oocytes, cultured in standard incubation, be optimised?SUMMARY ANSWERThe optimum time to perform fertilisation assessment for oocytes cultured in standard incubation is 16.5  ± 0.5 h post-insemination (hpi), and the current consensus requires modification in order to minimise the chance of fertilisation being missed.WHAT IS KNOWN ALREADYTime-lapse incubation allows the embryologist to retrospectively review collated images of oocytes and embryos to capture important embryological observations that may...
Source: Human Reproduction - September 18, 2021 Category: Reproduction Medicine Source Type: research

Comparing endometriotic lesions with eutopic endometrium: time to shift focus?
This article explores the histological features of endometriotic lesions, highlighting their sometimes underappreciated heterogeneity. We note the variability in evidence for and against the menstrual cycle responsiveness of lesions and consider the utility of drawing parallels between endometriotic lesions and eutopic endometrium. We ask whether histopathologic features beyond just the presence/absence of endometrial-like glands and/or stroma could help improve disease stratification. At the same time, we acknowledge the desire of many clinicians and patients to avoid invasive surgery thereby limiting the ability to histo...
Source: Human Reproduction - September 18, 2021 Category: Reproduction Medicine Source Type: research

Successful pregnancy after ovulation induction with human chorionic gonadotropin in a woman with selective luteinising hormone deficiency
ABSTRACTSelective LH deficiency has been described in several men, but only in two women who presented normal pubertal development but secondary amenorrhoea due to anovulation. Despite its rarity, this condition represents a valuable model for studying the processes regulated by FSH or LH during late folliculogenesis and ovulation in humans. A woman previously diagnosed with selective LH deficiency due to a homozygous germline splice site mutation inLHB (IVS2  + 1G→C mutation) was submitted to an individualised ovarian induction protocol, first with recombinant LH and then with highly purified urinary hCG. Ovarian fo...
Source: Human Reproduction - September 18, 2021 Category: Reproduction Medicine Source Type: research

Genetic diagnosis, sperm phenotype and ICSI outcome in case of severe asthenozoospermia with multiple morphological abnormalities of the flagellum
AbstractSTUDY QUESTIONAre ICSI outcomes impaired in cases of severe asthenozoospermia with multiple morphological abnormalities of the flagellum (MMAF phenotype)?SUMMARY ANSWERDespite occasional technical difficulties, ICSI outcomes for couples with MMAF do not differ from those of other couples requiring ICSI, irrespective of the genetic defect.WHAT IS KNOWN ALREADYSevere asthenozoospermia, especially when associated with the MMAF phenotype, results in male infertility. Recent findings have confirmed that a genetic aetiology is frequently responsible for this phenotype. In such situations, pregnancies can be achieved usin...
Source: Human Reproduction - September 16, 2021 Category: Reproduction Medicine Source Type: research

Pregnancy and pregnancy outcomes after hematopoietic stem cell transplantation in childhood: a cross-sectional survey of the EBMT Pediatric Diseases Working Party
AbstractSTUDY QUESTIONWhat are the characteristics of patients with conceptions transplanted in childhood and adolescence?SUMMARY ANSWERInsemination and conception after hematopoietic stem cell transplantation (HCT) in childhood or adolescence was possible, even after myeloablative conditioning regimes, although some patients required reproductive medicine support.WHAT IS KNOWN ALREADYPreparative regimens of HCT are highly gonadotoxic, which leads to gonadal failure and pubertal development disorders. There are few population-based studies assessing the risk of future infertility in children after HCT.STUDY DESIGN, SIZE, D...
Source: Human Reproduction - September 16, 2021 Category: Reproduction Medicine Source Type: research

Corrigendum. Nonvisualized palpable bowel endometriotic satellites
Hum Reprod 2021;36:656 –665 (Source: Human Reproduction)
Source: Human Reproduction - September 14, 2021 Category: Reproduction Medicine Source Type: research

Outcomes of SARS-CoV-2 infected pregancies after medically assisted reproduction
AbstractSTUDY QUESTIONWhat is the impact of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection on the outcome of a pregnancy after medically assisted reproduction (MAR)?SUMMARY ANSWEROur results suggest that MAR pregnancies are not differentially affected by SARS-CoV-2 infection compared to spontaneous pregnancies.WHAT IS KNOWN ALREADYInformation on the effects of coronavirus disease 2019 (COVID-19) on pregnancy after MAR is scarce when women get infected during MAR or early pregnancy, even though such information is vital for informing women seeking pregnancy.STUDY DESIGN, SIZE, DURATIONData from SARS-...
Source: Human Reproduction - September 13, 2021 Category: Reproduction Medicine Source Type: research

The expression and activity of Toll-like receptors in the preimplantation human embryo suggest a new role for innate immunity
AbstractSTUDY QUESTIONIs the innate immunity system active in early human embryo development?SUMMARY ANSWERThe pattern recognition receptors and innate immunity Toll-like receptor (TLR) genes are widely expressed in preimplantation human embryos and the pathway appears to be active in response to TLR ligands.WHAT IS KNOWN ALREADYEarly human embryos are highly sensitive to their local environment, however relatively little is known about how embryos detect and respond to specific environmental cues. While the maternal immune response is known to be key to the establishment of pregnancy at implantation, the ability of human ...
Source: Human Reproduction - September 13, 2021 Category: Reproduction Medicine Source Type: research

The structure of CatSper is revealed: happy days for sperm biology
CatSpercation channel of spermhyperactivationacrosome reactionprogesteronecalcium influxspermatozoainfertilitycontraceptionsperm function (Source: Human Reproduction)
Source: Human Reproduction - September 7, 2021 Category: Reproduction Medicine Source Type: research