Validation of improved automated nucleic acid extraction methods for direct detection of polioviruses for global polio eradication
J Virol Methods. 2024 Mar 6;326:114914. doi: 10.1016/j.jviromet.2024.114914. Online ahead of print.ABSTRACTPolioviruses (PV), the main causative agent of acute flaccid paralysis (AFP), are positive-sense single-stranded RNA viruses of the family Picornaviridae. As we approach polio eradication, accurate and timely detection of poliovirus in stool from AFP cases becomes vital to success for the eradication efforts. Direct detection of PV from clinical diagnostic samples using nucleic acid (NA) extraction and real-time reverse transcriptase polymerase chain reaction (rRT-PCR) instead of the current standard method of virus i...
Source: Journal of Virological Methods - March 8, 2024 Category: Virology Authors: Stacey Jeffries Miles Chelsea Harrington Hong Sun Ashley Deas M Steven Oberste W Allan Nix Everardo Vega Nancy Gerloff Source Type: research
Prevalence of vaccine-derived poliovirus in sewage waters in Maiduguri, Borno State, Nigeria
Infez Med. 2024 Mar 1;32(1):90-98. doi: 10.53854/liim-3201-12. eCollection 2024.ABSTRACTAfter a long global battle with wild poliovirus, the virus has been defeated through researches and vaccination using the oral polio vaccine and inactivated polio vaccine as well as sensitization. The issue that is now of global concern is that of vaccine-derived poliovirus which emerged from the unstable oral polio vaccine. Ninety sewage water samples were collected from slums in Maiduguri using grab method, concentrated using two phase separation method and subjected to intratypic differentiation and vaccine-derived poliovirus screeni...
Source: Infezioni in Medicina - March 8, 2024 Category: Infectious Diseases Authors: Sakinatu Buba Bislava Aliyu Daja Bamidele Soji Oderinde Sani Muhammad Uzairu Source Type: research
