Impact of different sterilisation techniques on sorption and NER formation of test chemicals in soil
In this study, experiments in accordance with OECD 307 and OECD 106 guidelines were performed with two soils covering wide range of soil characteristics and treated with the three sterilisation techniques autoclaving, gamma(γ)-radiation and adding 1% (w/w) sodium azide. As a test item, 14C-labelled phenanthrene and bromoxynil was used for OECD 307 test, whereas non-labelled phenanthrene and atrazine was used for OECD 106. The sterilisation efficiencies were investigated using traditional viable plate count and molecular approaches (RNA extraction method). The results suggest that none of the tested techniques resulted in ...
Source: Chemosphere - April 6, 2024 Category: Chemistry Authors: Rebecca S üßmuth Prasit Shrestha Cecilia Andrea Diaz Navarrete Franziska-Frederike Wege Christine Achten Dieter Hennecke Source Type: research
Impact of different sterilisation techniques on sorption and NER formation of test chemicals in soil
In this study, experiments in accordance with OECD 307 and OECD 106 guidelines were performed with two soils covering wide range of soil characteristics and treated with the three sterilisation techniques autoclaving, gamma(γ)-radiation and adding 1% (w/w) sodium azide. As a test item, 14C-labelled phenanthrene and bromoxynil was used for OECD 307 test, whereas non-labelled phenanthrene and atrazine was used for OECD 106. The sterilisation efficiencies were investigated using traditional viable plate count and molecular approaches (RNA extraction method). The results suggest that none of the tested techniques resulted in ...
Source: Chemosphere - April 6, 2024 Category: Chemistry Authors: Rebecca S üßmuth Prasit Shrestha Cecilia Andrea Diaz Navarrete Franziska-Frederike Wege Christine Achten Dieter Hennecke Source Type: research
Current situation of lead (Pb) exposure in raptors and waterfowl in Japan and difference in sensitivity to in vitro lead exposure among avian species
Environ Pollut. 2024 Apr 4:123907. doi: 10.1016/j.envpol.2024.123907. Online ahead of print.ABSTRACTAlthough lead (Pb) poisoning in wild birds has been considered a serious problem in Japan for over 30 years, there is little information about Pb exposure and its sources throughout Japan except for Hokkaido. Furthermore, to identify and effectively prioritize the conservation needs of highly vulnerable species, differences in sensitivity to Pb exposure among avian species need to be determined. Therefore, we investigated the current situation of Pb exposure in raptors (13 species, N = 82), waterfowl (eight species, N = 44) ...
Source: Environmental Pollution - April 6, 2024 Category: Environmental Health Authors: Mizuki Maruyama Nana Ushine Yukiko Watanabe Chihiro Ishii Keisuke Saito Hidefumi Sakai Takeshi Kuritani Rio Doya Kohei Ogasawara Yoshinori Ikenaka Yared Beyene Yohannes Mayumi Ishizuka Shouta M M Nakayama Source Type: research
Impact of different sterilisation techniques on sorption and NER formation of test chemicals in soil
In this study, experiments in accordance with OECD 307 and OECD 106 guidelines were performed with two soils covering wide range of soil characteristics and treated with the three sterilisation techniques autoclaving, gamma(γ)-radiation and adding 1% (w/w) sodium azide. As a test item, 14C-labelled phenanthrene and bromoxynil was used for OECD 307 test, whereas non-labelled phenanthrene and atrazine was used for OECD 106. The sterilisation efficiencies were investigated using traditional viable plate count and molecular approaches (RNA extraction method). The results suggest that none of the tested techniques resulted in ...
Source: Chemosphere - April 6, 2024 Category: Chemistry Authors: Rebecca S üßmuth Prasit Shrestha Cecilia Andrea Diaz Navarrete Franziska-Frederike Wege Christine Achten Dieter Hennecke Source Type: research
Impact of different sterilisation techniques on sorption and NER formation of test chemicals in soil
In this study, experiments in accordance with OECD 307 and OECD 106 guidelines were performed with two soils covering wide range of soil characteristics and treated with the three sterilisation techniques autoclaving, gamma(γ)-radiation and adding 1% (w/w) sodium azide. As a test item, 14C-labelled phenanthrene and bromoxynil was used for OECD 307 test, whereas non-labelled phenanthrene and atrazine was used for OECD 106. The sterilisation efficiencies were investigated using traditional viable plate count and molecular approaches (RNA extraction method). The results suggest that none of the tested techniques resulted in ...
Source: Chemosphere - April 6, 2024 Category: Chemistry Authors: Rebecca S üßmuth Prasit Shrestha Cecilia Andrea Diaz Navarrete Franziska-Frederike Wege Christine Achten Dieter Hennecke Source Type: research
Polyvinyl chloride solvent cement poisoning: a case report
ConclusionIngestion of a sufficient quantity ofS could result in gut absorption with central nervous system depression, coma, and even death. No antidote is available for toxicity, and management is largely supportive. As witnessed in our patient, chemical laryngitis and upper airway inflammation may lead to upper airway obstruction. Chemical reactions with medical equipment may lead to unforeseen outcomes. (Source: Journal of Medical Case Reports)
Source: Journal of Medical Case Reports - April 6, 2024 Category: General Medicine Source Type: research
Autophagic-lysosomal damage induced by swainsonine is protected by trehalose through activation of TFEB-regulated pathway in renal tubular epithelial cells
In this study, we investigate the mechanism of protective effects of trehalose using renal tubular epithelial cells. The results showed that SW induced an increase in the expression level of microtubule-associated protein light chain 3-II and p62 proteins and a decrease in the expression level of ATPase H+ transporting V1 Subunit A, Cathepsin B, Cathepsin D, lysosome-associated membrane protein 2 and TFEB proteins in renal tubular epithelial cells in a time and dose-dependent manner suggesting TFEB-regulated lysosomal pathway is adversely affected by SW. Conversely, treatment with trehalose, a known activator of TFEB promo...
Source: Chemico-Biological Interactions - April 5, 2024 Category: Molecular Biology Authors: Shuhang Zhang Hai Yin Yiqingqing Zhang Yanli Zhu Xueyao Zhu Wenting Zhu Lihui Tang Yiling Liu Kexin Wu Baoyu Zhao Yanan Tian Hao Lu Source Type: research
Nanocrocin Protective Effects on Paraquat-Induced Oxidative Stress in the MRC-5 Cell Line
This study aimed to investigate the effects of crocin (CCN) and nano crocin (NCCN) on PQ -induced toxicity in the MRC-5 cell line. The results showed that the particle size of NCCN was 140.3 ± 18.0 nm, and the zeta potential of the optimal crocin-loaded niosomes was 23.4 ± 2.8 mV. The NCCN was more effective than CCN in the inhibition of PQ-induced toxicity. Treatment of the MRC-5 cells leads to a decrease in ROS and an increase in SOD, CAT, GPX, and TAC levels in PQ-CCN and PQ-NCCN groups compared with the PQ group. These changes tended to be positively associated with the NCCN compared to CCN. Overall, NCCN was more ef...
Source: Clinical Biochemistry - April 5, 2024 Category: Biochemistry Authors: Akram Oftadeh Harsin Farzin Firozian Amir Nili Ahmadabadi Meysam Soleimani Akram Ranjbar Source Type: research
Nanozyme-assisted molecularly imprinted polymer-based indirect competitive ELISA for the detection of marine biotoxin
In this study, we developed an affinity peptide-imprinted polymer-based indirect competitive ELISA (ic-ELISA) without using enzyme-toxin conjugates. AuNP/Co3O4@Mg/Al cLDH was synthesized by calcining AuNP/ZIF-67@Mg/Al LDH, which was obtained by combining AuNPs, ZIF-67, and flower-like Mg/Al LDH. This synthesized nanozyme exhibited high catalytic activity (Km = 0.24 mM for TMB and 132.5 mM for H2O2). The affinity peptide-imprinted polymer (MIP) was imprinted with an STX-specific template peptide (STX MIP) on a multi-well microplate and then reacted with an STX-specific signal peptide (STX SP). The interaction between the ST...
Source: Biosensors and Bioelectronics - April 5, 2024 Category: Biotechnology Authors: Chae Hwan Cho Ji Hong Kim Navnath S Padalkar Y Veera Manohara Reddy Tae Jung Park Jinyoung Park Jong Pil Park Source Type: research
Autophagic-lysosomal damage induced by swainsonine is protected by trehalose through activation of TFEB-regulated pathway in renal tubular epithelial cells
In this study, we investigate the mechanism of protective effects of trehalose using renal tubular epithelial cells. The results showed that SW induced an increase in the expression level of microtubule-associated protein light chain 3-II and p62 proteins and a decrease in the expression level of ATPase H+ transporting V1 Subunit A, Cathepsin B, Cathepsin D, lysosome-associated membrane protein 2 and TFEB proteins in renal tubular epithelial cells in a time and dose-dependent manner suggesting TFEB-regulated lysosomal pathway is adversely affected by SW. Conversely, treatment with trehalose, a known activator of TFEB promo...
Source: Chemico-Biological Interactions - April 5, 2024 Category: Molecular Biology Authors: Shuhang Zhang Hai Yin Yiqingqing Zhang Yanli Zhu Xueyao Zhu Wenting Zhu Lihui Tang Yiling Liu Kexin Wu Baoyu Zhao Yanan Tian Hao Lu Source Type: research
Nanocrocin Protective Effects on Paraquat-Induced Oxidative Stress in the MRC-5 Cell Line
This study aimed to investigate the effects of crocin (CCN) and nano crocin (NCCN) on PQ -induced toxicity in the MRC-5 cell line. The results showed that the particle size of NCCN was 140.3 ± 18.0 nm, and the zeta potential of the optimal crocin-loaded niosomes was 23.4 ± 2.8 mV. The NCCN was more effective than CCN in the inhibition of PQ-induced toxicity. Treatment of the MRC-5 cells leads to a decrease in ROS and an increase in SOD, CAT, GPX, and TAC levels in PQ-CCN and PQ-NCCN groups compared with the PQ group. These changes tended to be positively associated with the NCCN compared to CCN. Overall, NCCN was more ef...
Source: Clinical Biochemistry - April 5, 2024 Category: Biochemistry Authors: Akram Oftadeh Harsin Farzin Firozian Amir Nili Ahmadabadi Meysam Soleimani Akram Ranjbar Source Type: research
Nanozyme-assisted molecularly imprinted polymer-based indirect competitive ELISA for the detection of marine biotoxin
In this study, we developed an affinity peptide-imprinted polymer-based indirect competitive ELISA (ic-ELISA) without using enzyme-toxin conjugates. AuNP/Co3O4@Mg/Al cLDH was synthesized by calcining AuNP/ZIF-67@Mg/Al LDH, which was obtained by combining AuNPs, ZIF-67, and flower-like Mg/Al LDH. This synthesized nanozyme exhibited high catalytic activity (Km = 0.24 mM for TMB and 132.5 mM for H2O2). The affinity peptide-imprinted polymer (MIP) was imprinted with an STX-specific template peptide (STX MIP) on a multi-well microplate and then reacted with an STX-specific signal peptide (STX SP). The interaction between the ST...
Source: Biosensors and Bioelectronics - April 5, 2024 Category: Biotechnology Authors: Chae Hwan Cho Ji Hong Kim Navnath S Padalkar Y Veera Manohara Reddy Tae Jung Park Jinyoung Park Jong Pil Park Source Type: research
Sudan black poisoning resulted in methemoglobinemia in a baby with congenital chyloperitoneum
Key Clinical MessageTreatment of congenital chyloperitoneum is a challenge. Conservative methods may be ineffective. Preoperative visualization of the site of lymphatic leakage is crucial, but radiological imaging is technically complicated and may not provide sufficient information, especially in small patients. To ease the detection of lymphatic leakage during surgery, preoperative feeding with fat-rich formula with Sudan Black has been recommended. However, administration of Sudan Black may result in life-threatening methemoglobinemia and liver damage without any advantage of revealing leakage during surgery. We recomme...
Source: Clinical Case Reports - April 5, 2024 Category: General Medicine Authors: Helgi Padari,
Karin Kipper,
Imbi Eelm äe,
Jekaterina Nerman,
Mare Lintrop,
Tuuli Metsvaht Tags: CASE REPORT Source Type: research
Paramao root oil: a novel culprit in salicylate poisoning
. (Source: Clinical Toxicology)
Source: Clinical Toxicology - April 5, 2024 Category: Toxicology Authors: Bashir ChakarSarah KabirChianna DaneNicholas ProschogoAndrew Dawsona Westmead Hospitalb Nepean Hospitalc NSW Poisons Information Centred Royal Prince Alfred Hospitale School of Chemistry, University of Sydneyf Edith Collins Centre, University of Sydney Source Type: research
Development and validation of streptavidin-biotin-based double antibody sandwich ELISA for ricin diagnosis
CONCLUSION: The developed assay is highly sensitive and specific for detecting ricin and is not cross-reactive with other similar types of toxins. The assay can detect ricin in spiked plasma samples, so it has the potential to be used for the analysis of clinical samples after ricin poisoning.PMID:38574703 | DOI:10.1016/j.intimp.2024.111986 (Source: International Immunopharmacology)
Source: International Immunopharmacology - April 4, 2024 Category: Allergy & Immunology Authors: Shivani Dixit Jagrati Parashar Ram Kumar Dhaked Abdhesh Kumar Nandita Saxena Source Type: research
