The Challenge that Chlorine Presented during the Development of a Benzodiazepine Assay.
Abstract During the routine validation of a benzodiazepine method (performed on a Liquid Chromatography - Tandem Mass Spectrometer), it was noted that lorazepam, triazolam, and α-hydroxytriazolam showed a quadratic shift/bias in the calibration curve, particularly at high concentrations. The ultimate cause of this bias was determined to be due to the natural presence of chlorine (Cl) isotopes (35Cl and 37Cl) in these benzodiazepines. The presence of the heavy (37Cl) isoforms of Cl resulted in the analyte's mass being the same as the internal standard which, in turn, caused the internal standard to appear &qu...
Source: Clinical Biochemistry - December 3, 2019 Category: Biochemistry Authors: Lahr RG, Jannetto PJ, Langman LJ Tags: Clin Biochem Source Type: research

Gender-Specific Reference Values for High-Sensitivity Cardiac Troponin T and I in Well-Phenotyped Healthy Individuals and Validity of High-Sensitivity Assay Designation.
CONCLUSIONS: In a well-phenotyped healthy cohort, reference values for hs-cTnT were slightly higher, whereas hs-cTnI cut-offs were considerably lower than previously observed. Gender differences were more pronounced in hs-cTnI than in hs-cTnT and were further reduced for hs-cTnT by application of stricter health definition criteria. Contrary to hs-cTnI, hs-cTnT fulfilled criteria for hs designation for both genders. PMID: 31786204 [PubMed - as supplied by publisher] (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - November 28, 2019 Category: Biochemistry Authors: Giannitsis E, Mueller-Hennessen M, Zeller T, Schuebler A, Aurich M, Bieneram M, Vafaie M, Stoyanov K, Ochs M, Riffel J, Mereles D, Blankenberg S, Katus HA Tags: Clin Biochem Source Type: research

Emerging biomarkers for cardiac arrhythmias.
Abstract Cardiac arrhythmias are associated with substantial morbidity and mortality. Recent advances in the pathophysiological understanding of cardiac arrhythmia indicate that inflammation, fibrosis, and even autoimmune mechanisms could facilitate the development of arrhythmias by interfering either with fibroblast activation-related electrical remodeling or with the function of different cardiac ion channels, leading to the emerging concepts of autoimmune and inflammatory channelopathies. In this descriptive review, we considered recent data of the literature focusing on biomarkers reflecting the degree of infl...
Source: Clinical Biochemistry - November 28, 2019 Category: Biochemistry Authors: Hammerer-Lercher A, Namdar M, Vuilleumier N Tags: Clin Biochem Source Type: research

Automatic laboratory interventions to unmask and treat hypomagnesemia in the Emergency Department.
a-Salinas C Abstract INTRODUCTION: The significance of hypomagnesemia and the need for treatment are under-recognized in clinical practice. Our objective was to design, establish, and test two interventions to screen for patients with hypomagnesemia and increase the rate of treatment of hypomagnesemia in the emergency department (ED). MATERIAL AND METHODS: A prospective two-year study was conducted. The Laboratory Information System was set to automatically order plasma magnesium in ED patients with plasma calcium
Source: Clinical Biochemistry - November 28, 2019 Category: Biochemistry Authors: Salinas M, Flores E, López-Garrigós M, Puche C, Leiva-Salinas C Tags: Clin Biochem Source Type: research

Reduction of AHI1 in the serum of Taiwanese with probable Alzheimer's disease.
CONCLUSION: An early event of AHI1 reduction in the body of AD patients was observed. Serum AHI1 may be valuable for early diagnosis of AD. PMID: 31786207 [PubMed - as supplied by publisher] (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - November 28, 2019 Category: Biochemistry Authors: Sheu JJ, Yang LY, Renuka Sanotra M, Wang ST, Lu HT, Sook Yee Kam R, Hsu IU, Kao SH, Lee CK, Chang-Cheng Shieh J, Lin YF Tags: Clin Biochem Source Type: research

Identification of germline pathogenic variants in DNA damage repair genes by a next-generation sequencing multigene panel in BRCAX patients.
CONCLUSIONS: We identified that 8% of BRCAX patients were carriers of pathogenic variants in genes other than BRCA1 and BRCA2. Therefore, wide gene panels, including clinically actionable genes, should be routinely used in the screening of HBOC in our population. We observed differences from other studies in the prevalence of mutated genes, most likely due to differences in the selection criteria of the probands and in the population analyzed. The high incidence of deleterious variant detection in PALB2 supports its significant role in breast cancer susceptibility and reinforces its inclusion in the HBOC genetic diagnostic...
Source: Clinical Biochemistry - November 28, 2019 Category: Biochemistry Authors: Rodríguez-Balada M, Roig B, Melé M, Albacar C, Serrano S, Salvat M, Querol M, Borràs J, Martorell L, Gumà J Tags: Clin Biochem Source Type: research

Establishing reference intervals of coagulation indices based on the ACL Top 700 system for children in Southwestern Fujian, China.
CONCLUSIONS: There were minor changes in the RIs of coagulation indices for children. The RIs of PT, INR, APTT, TT, and FIB must be grouped by age. The RIs of coagulation indices for children were different from those for adults; therefore, establishing separate RIs for children is necessary. PMID: 31770522 [PubMed - as supplied by publisher] (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - November 23, 2019 Category: Biochemistry Authors: Zhang H, Li J, Chen H, Wu X Tags: Clin Biochem Source Type: research

An isotope dilution LC-MS/MS based candidate reference method for the quantification of cyclosporine A, tacrolimus, sirolimus and everolimus in human whole blood.
d U Abstract An isotope dilution LC-MS/MS based candidate reference measurement procedure for the quantification of cyclosporine A, tacrolimus, sirolimus and everolimus in human whole blood is presented to be used for evaluation and standardization of routine assays applied for therapeutic drug monitoring. The assay allows baseline separation of the four immunosuppressive drugs within a total runtime of 9 minutes using a C4 reversed phase column. Sample preparation is based on protein precipitation with zinc sulphate followed by purification with solid phase extraction. Reference materials used in this reference m...
Source: Clinical Biochemistry - November 23, 2019 Category: Biochemistry Authors: Taibon J, van Rooij M, Schmid R, Singh N, Albrecht E, Anne Wright J, Geletneky C, Schuster C, Mörlein S, Vogeser M, Seger C, Pongratz S, Kobold U Tags: Clin Biochem Source Type: research

Plasma proteomics-based identification of novel biomarkers in early gastric cancer.
CONCLUSION: The proteins identified by plasma proteomics using LC-MS/MS combined with TMT labeling could help distinguish EGC from healthy controls. PMID: 31765635 [PubMed - as supplied by publisher] (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - November 22, 2019 Category: Biochemistry Authors: Zhou B, Zhou Z, Chen Y, Deng H, Cai Y, Rao X, Yin Y, Rong L Tags: Clin Biochem Source Type: research

Evolving Hyperthyroidism?
PMID: 31765636 [PubMed - as supplied by publisher] (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - November 22, 2019 Category: Biochemistry Authors: Meng QH Tags: Clin Biochem Source Type: research

Genetic and phenotypic analysis of a rare asymptomatic case of a homozygous Chinese G γ+(Aγδβ)0-thalassemia deletion in a Chinese family.
CONCLUSIONS: Thus far, this is the first study to describe the molecular characterization of a homozygous Chinese Gγ+(Aγδβ)0-thalassemia patient who exhibits no clinical symptoms. Our findings suggest that coinheritance of α-thalassemia or HBS1L-MYB locus variants may affect the clinical severity of Chinese Gγ+(Aγδβ)0-thalassemia. We conclude that the molecular examination of genetic determinants known to be associated with clinical outcomes in Chinese Gγ+(Aγδβ)0-thalassemia should be emphasized. PMID: 31765637 [PubMed - as supplied by publishe...
Source: Clinical Biochemistry - November 22, 2019 Category: Biochemistry Authors: Du L, Qin D, Wang J, Yu L, Yao C, Liu L, Zhang Y, Hu T, Yuan T, Liang J, Yin A Tags: Clin Biochem Source Type: research

Very high levels of PSA in patients with cardiogenic shock: report of four clinical cases.
lina R, Filella X Abstract Prostate-specific antigen (PSA) is the tumor marker most widely used in conjunction with digital rectal examination (DRE) for the early detection of prostate cancer (PCa). Due to its limitations, especially the high rate of false positive (FP) results, PSA screening of transplant candidates is a controversial issue. Moreover, obtaining a FP result in the PCa screening of heart transplant candidates may lead to potentially harmful effects. Although most of the factors that may cause PSA FP results are well known, FP results related to cardiogenic shock, a common indication for heart trans...
Source: Clinical Biochemistry - November 22, 2019 Category: Biochemistry Authors: Fernández-Galán E, Augé JM, Molina R, Filella X Tags: Clin Biochem Source Type: research

Multi-platform analytical evaluation of the Beckman Coulter Access high-sensitivity troponin I assay across different laboratory sites using Barricor plasma.
Higgins T Abstract OBJECTIVES: Previous analytical evaluations of the Beckman Access high sensitivity troponin (hsTn) I assay have focused on single platforms and laboratories. The purpose of this study was to determineassay robustness across different platform models at multiple sites, platform-specific characteristics, and equivalence to other hsTn methods to be used in a large laboratory network. METHODS: Barricor plasma was used to assess imprecision, linearity, sensitivity (limit of blank and detection, LOB/LOD), and comparability to the conventional AccuTnI+3 and other hsTn assays. Studies were conducte...
Source: Clinical Biochemistry - November 16, 2019 Category: Biochemistry Authors: Raizman JE, Tsui AKY, Goudreau BL, Füzéry AK, Estey M, Sadrzadeh H, Higgins T Tags: Clin Biochem Source Type: research

Automated Analysis of Dried Urine Spot (DUS) Samples for Toxicology Screening.
CONCLUSION: Using a robotic DUS sampling an inline HTLC-MS/MS system, we have developed and validated a fully-automated and robust method for multi-analyte detection of drugs of abuse in dried urine specimens. PMID: 31707014 [PubMed - as supplied by publisher] (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - November 7, 2019 Category: Biochemistry Authors: Pablo A, Breaud AR, Clarke W Tags: Clin Biochem Source Type: research

Comparison of early cardiovascular risk among Brazilian and African university students.
CONCLUSIONS: The anthropometric and biochemical parameters of Brazilians, especially men, predispose them to greater risks of cardiovascular diseases. PMID: 31678428 [PubMed - as supplied by publisher] (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - October 31, 2019 Category: Biochemistry Authors: Laurindo Holanda de Sousa E, Emanuel Andrade Cavalcante J, Freire de Sousa D, Magalhães Ferreira J, Rarison Cavalcante Meneses R, Lopes Sousa D, Jordan Xavier Silva A, Rigoberto Barbosa Xavier Filho R, da Silva Dos Santos E, Havt A, Raquel Teixeira Damas Tags: Clin Biochem Source Type: research

Characterization of biotin interference in 21 Vitros 5600 immunoassays and risk mitigation for patient safety at a large academic medical center.
CONCLUSIONS: Analytical bias caused by biotin interference is dependent on biotin concentration but independent of analyte concentration for immunometric methods using the Vitros 5600, and dependent on both biotin and analyte concentration for competitive immunoassays. Multi-disciplinary education and a lab streptavidin-microparticle pretreatment method help mitigate risk of erroneous results due to biotin interference for patient safety. PMID: 31677994 [PubMed - as supplied by publisher] (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - October 30, 2019 Category: Biochemistry Authors: Stieglitz HM, Korpi-Steiner N Tags: Clin Biochem Source Type: research

Serum human neutrophil lipocalin: an effective biomarker for diagnosing bacterial infections.
Abstract BACKGROUND: Human neutrophil lipocalin (HNL) is used as a novel biomarker for infections. However, only a few studies have focused on the usefulness of HNL. The purpose of this study was to evaluate the diagnostic efficiency of HNL for identifying bacterial infections and to compare HNL with procalcitonin (PCT) and C-reactive protein (CRP). METHODS: Hospital patients with acute infections of bacterial origin (n=439), viral origin (n=71), and healthy volunteers (n=67) were included in the study. The infection status of each patient was verified using microbiological, serological, and PCR testing. Addi...
Source: Clinical Biochemistry - October 28, 2019 Category: Biochemistry Authors: Fang C, Wang Z, Dai Y, Chang W, Sun L, Ma X Tags: Clin Biochem Source Type: research

Implementation of an educational province-wide intervention to reduce redundant vitamin B12 testing: a cross-sectional study.
CONCLUSIONS: An educational intervention aimed at limiting inappropriate vitamin B12 testing in Alberta did not alter testing as desired. Multiple utilization management strategies and a longer observation period may be needed to reduce redundant vitamin B12 testing. PMID: 31672645 [PubMed - as supplied by publisher] (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - October 28, 2019 Category: Biochemistry Authors: Gill P, Guo M, Lau CK, Naugler C Tags: Clin Biochem Source Type: research

Retrospective Evaluation of 99th Percentile hCG Results to Adjust Clinical Decision Points.
Abstract While accurate measurement of chorionic gonadotropin (hCG) is necessary, so are appropriate clinical decision points (CDPs) for patients of all ages. The CDP for hCG is intended to identify early pregnancy in patients of child bearing age; non-pregnant patients who are older frequently yield hCG results>5 IU/L, making the use of a low hCG CDP problematic for these patients. Using a retrospective review of all hCG results generated over a 32-month period, 8507 hCG results from non-pregnant females of all ages were analyzed. Patients
Source: Clinical Biochemistry - October 28, 2019 Category: Biochemistry Authors: Tacker DH Tags: Clin Biochem Source Type: research

Prognostic Urinary miRNAs for the Assessment of Small Renal Masses.
CONCLUSIONS: Our in-depth miRNA profiling approach identified novel biomarkers for early-stage ccRCC-SRMs. Pretreatment characterization of urinary miRNAs may provide insight into early RCC progression and could potentially aid clinical decision-making, improving patient management and reducing overtreatment. PMID: 31672647 [PubMed - as supplied by publisher] (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - October 28, 2019 Category: Biochemistry Authors: Di Meo A, Brown MD, Finelli A, Jewett MAS, Diamandis EP, Yousef GM Tags: Clin Biochem Source Type: research

Title: Comparison of Plasma, Saliva, and Hair Lamotrigine Concentrations.
Abstract BACKGROUND: In some clinical situations (pregnancy, aging, drug resistance, toxicity), measurements of lamotrigine plasma levels may be reliable. Limited studies indicate that saliva and hair could be alternative sources for monitoring lamotrigine therapy. The drug content in hair can also be used to assess the history of drug therapy and to ascertain long-term patient compliance. The aims of this study were to 1) determine the correlations among plasma, saliva, and hair lamotrigine concentrations, 2) evaluate saliva as an alternative matrix for monitoring drug levels and 3) evaluate hair as a source of i...
Source: Clinical Biochemistry - October 28, 2019 Category: Biochemistry Authors: Kuczynska J, Karas-Ruszczyk K, Zakrzewska A, Dermanowski M, Sienkiewicz-Jarosz H, Kurkowska-Jastrzebska I, Bienkowski P, Konopko M, Dominiak M, Mierzejewski P Tags: Clin Biochem Source Type: research

Diagnostic value of serum neutrophil gelatinase-associated lipocalin, interleukin-6 and anti-citrullinated alpha-enolase peptide 1 for lower respiratory tract infections.
Abstract OBJECTIVES: The aim of this study was to explore the auxiliary diagnostic value of neutrophil gelatinase-associated lipocalin (NGAL) and anti-citrullinated alpha-enolase peptide 1 (CEP-1) in lower respiratory tract infections (LRTIs). METHODS: Blood samples were collected from 99 in-patients with LRTIs [62 community-acquired pneumonia (CAP), 14 acute exacerbated chronic obstructive pulmonary diseases (AECOPD), 23 other diseases] and 50 healthy subjects. NGAL, CEP-1 and IL-6 were measured and compared. IL-6 was tested by electrochemiluminescence assay kit on Roche E601 immunology analyzer, CEP-1 was a...
Source: Clinical Biochemistry - October 28, 2019 Category: Biochemistry Authors: Liu C, Wang F, Cui L, Zhou J, Xu Z Tags: Clin Biochem Source Type: research

Endostatin predicts mortality in patients with acute dyspnea - a cohort study of patients seeking care in emergency departments.
Ruge T Abstract BACKGROUND: Increased levels of circulating endostatin predicts cardiovascular morbidity and impaired kidney function in the general population. The utility of endostatin as a risk marker for mortality in the emergency department (ED) has not been reported. AIM: Our main aim was to study the association between plasma endostatin and 90-day mortality in an unselected cohort of patients admitted to the ED for acute dyspnea. Design Circulating endostatin was analyzed in plasma from 1710 adults and related to 90-day mortality in Cox proportional hazard models adjusted for age, sex, body mass inde...
Source: Clinical Biochemistry - October 28, 2019 Category: Biochemistry Authors: Carlsson AC, Wessman T, Larsson A, Leijonberg G, Tofik R, Ärnlöv J, Melander O, Ruge T Tags: Clin Biochem Source Type: research

High Clusterin (CLU) mRNA expression levels in tumors of colorectal cancer patients predict a poor prognostic outcome.
CONCLUSIONS: High CLU mRNA levels in CRC tumors can act as a new adverse prognostic biomarker of DFS and OS for CRC, independent of clinicopathological and biological features of the patient. PMID: 31672651 [PubMed - as supplied by publisher] (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - October 28, 2019 Category: Biochemistry Authors: Artemaki PI, Sklirou AD, Kontos CK, Liosi AA, Gianniou DD, Papadopoulos IN, Trougakos IP, Scorilas A Tags: Clin Biochem Source Type: research

Serum profile of transferrin isoforms in rheumatoid arthritis treated with biological drugs.
CONCLUSIONS: This study indicates that treatment with rituximab of RA patients alters the serum profile of transferrin isoforms. Tri-, tetra- and pentasialotransferrin relative concentrations measurements can be a useful tool to monitor therapy. PMID: 31672652 [PubMed - as supplied by publisher] (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - October 28, 2019 Category: Biochemistry Authors: Cylwik B, Gruszewska E, Gindzienska-Sieskiewicz E, Kowal-Bielecka O, Chrostek L Tags: Clin Biochem Source Type: research

Plasma circular RNA panel acts as a novel diagnostic biomarker for colorectal cancer.
CONCLUSION: Our study provides the first identification of a panel of three plasma circRNAs that could serve as a novel and independent diagnostic biomarker for CRC. PMID: 31669510 [PubMed - as supplied by publisher] (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - October 26, 2019 Category: Biochemistry Authors: Lin J, Cai D, Li W, Yu T, Mao H, Jiang S, Xiao B Tags: Clin Biochem Source Type: research

Pivotal Findings for a High-Sensitivity Cardiac Troponin Assay: Results of the HIGH-US Study.
CONCLUSION: The Atellica IM TnIH assay is a high-sensitivity method and demonstrates clinical performance appropriate for AMI diagnosis. PMID: 31669511 [PubMed - as supplied by publisher] (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - October 26, 2019 Category: Biochemistry Authors: Christenson RH, Duh SH, Apple FA, Nowak R, Peacock WF, Limkakeng AT, Bostanian Z, Mohammad A, McCord J, deFilippi CR Tags: Clin Biochem Source Type: research

Estimating Short- and Long-term Reference Change Values and Index of Individuality for Tests of Platelet Function.
Abstract BACKGROUND: In order to manage risks of bleeding and thrombosis after some surgical procedures, platelet function is often measured repeatedly over days or weeks using laboratory tests of platelet function. To interpret test results in the perioperative period, it is necessary to understand analytical, biological and between-person variation. METHODS: We collected three separate blood specimens from 16 healthy volunteers on the first study day, and one additional specimen from each volunteer 1, 2, and 3 months later. Arachidonic acid-induced and adenosine diphosphate (ADP)-induced platelet function w...
Source: Clinical Biochemistry - October 26, 2019 Category: Biochemistry Authors: Katzman BM, Wockenfus AM, Scott RJ, Bryant SC, Jaffe AS, Karon BS Tags: Clin Biochem Source Type: research

A Novel Approach to Remove Interference of Therapeutic Monoclonal Antibody with Serum Protein Electrophoresis.
CONCLUSIONS: This demonstrates proof of principle that ASADA can be used to remove current and future tmAb from patient sera, regardless of platform. This research provides for accurate diagnosis, disease monitoring, and remission status in MM patients being treated with tmAb. PMID: 31669513 [PubMed - as supplied by publisher] (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - October 26, 2019 Category: Biochemistry Authors: Liu L, Shurin MR, Wheeler SE Tags: Clin Biochem Source Type: research

Comparison of the Quantum Blue ® Reader Point-of-Care system versus ELISA technique for therapeutic drug monitoring of Infliximab levels.
Comparison of the Quantum Blue® Reader Point-of-Care system versus ELISA technique for therapeutic drug monitoring of Infliximab levels. Clin Biochem. 2019 Oct 26;: Authors: Novakovic V, Abdija S, Larsen PB, Fenger M, Gredal L, Jacobsen KK Abstract BACKGROUND: Infliximab (IFX) is a monoclonal antibody used to treat patients with inflammatory bowel disease (IBD). For IFX therapeutic drug monitoring (TDM), the most commonly used analysis is enzyme-linked immunosorbent assays (ELISA) which do not allow results to be provided in real-time. The aim of this study was to compare the in-house ELISA (Promo...
Source: Clinical Biochemistry - October 26, 2019 Category: Biochemistry Authors: Novakovic V, Abdija S, Larsen PB, Fenger M, Gredal L, Jacobsen KK Tags: Clin Biochem Source Type: research

Multilevel qualification of a large set of blood gas analyzers: Which performance goals?
CONCLUSIONS: Our study is an example of the qualification of a set of point and underscores 1)The need for a unified qualification scheme when multiple analyzers are deployed simultaneously 2) analytical performance goals compatible with clinical use and the state of the art for all parameters. PMID: 31533025 [PubMed - as supplied by publisher] (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - September 15, 2019 Category: Biochemistry Authors: Kuster N, Bargnoux AS, Badiou S, Dupuy AM, Piéroni L, Cristol JP Tags: Clin Biochem Source Type: research

Reference change values of M-protein, free light chain and immunoglobulins in monoclonal gammopathy.
In this study, we aimed to calculate the variation in M-protein, free light chains (FLCs), and immunoglobulins in respective patients. DESIGN & METHODS: We analyzed the data of clinically stable patients with monoclonal gammopathy (MG), which were monitored for 7-years to determine the biological variations and reference change values (RCV) of serum M-protein, monoclonal serum FLCs and immunoglobulin (Ig) concentrations. Patients that were included in the study had no change in diagnosis and showed
Source: Clinical Biochemistry - September 14, 2019 Category: Biochemistry Authors: Evliyaoglu O, van Helden J, Jaruschewski S, Imöhl M, Weiskirchen R Tags: Clin Biochem Source Type: research

Establishment of reference intervals for thyroid hormones in premature infants beyond the first week of life using Beckman Coulter Unicel DxI 800.
CONCLUSIONS: Unlike those of full-term infants or adults, the reference intervals established in this study are applicable in premature infants. These results highlight the importance and complexity of establishing instrument-specific thyroid hormone reference intervals for preterm infants. PMID: 31499031 [PubMed - as supplied by publisher] (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - September 6, 2019 Category: Biochemistry Authors: Wang Y, Wu X, Hong K, Fu X, Chen T, Zhu M, Ye H Tags: Clin Biochem Source Type: research

PCSK9 and atherosclerosis burden in the coronary arteries of patients undergoing coronary angiography.
Abstract AIMS: To investigate the association between plasma proprotein convertase subtilisin/kexin type 9 (PCSK9) concentrations, current acute coronary syndrome (ACS), coronary artery disease (CAD) presence, severity and extension and the burden of coronary calcifications in patients with suspected CAD. METHODS AND RESULTS: One hundred and one patients, with or without current ACS, were recruited for this cross-sectional study. CAD presence was defined based on either the presence or absence of at least one significant (≥50%) CAD lesion (SCAD). CAD severity was classified according to the absence of coro...
Source: Clinical Biochemistry - September 4, 2019 Category: Biochemistry Authors: Panahi Y, Ghahrodi MS, Jamshir M, Safarpour MA, Bianconi V, Pirro M, Farahani MM, Sahebkar A Tags: Clin Biochem Source Type: research

Hb Vanvitelli: A new unstable α-globin chain variant causes undiagnosed chronic haemolytic anaemia when co-inherited with deletion - α3.7.
Hb Vanvitelli: A new unstable α-globin chain variant causes undiagnosed chronic haemolytic anaemia when co-inherited with deletion - α3.7. Clin Biochem. 2019 Sep 04;: Authors: Casale M, Cozzolino F, Scianguetta S, Pucci P, Monaco V, Sanchez G, Santoro C, Rubino R, Cannata M, Perrotta S Abstract Hb variants are structurally abnormal haemoglobins which can originate a wide range of phenotypes from clinically silent conditions to very severe disorders. In many cases, diagnosis is very difficult due to the instability of Hb mutants or the occurrence of misleading symptoms, such as cyanosis...
Source: Clinical Biochemistry - September 4, 2019 Category: Biochemistry Authors: Casale M, Cozzolino F, Scianguetta S, Pucci P, Monaco V, Sanchez G, Santoro C, Rubino R, Cannata M, Perrotta S Tags: Clin Biochem Source Type: research

Establishing thresholds and effects of gender, age, and season for thyroglobulin and thyroid peroxidase antibodies by mining real-world big data.
CONCLUSION: The levels of TG-Ab and TPO-Ab were significantly affected by gender, age, and season. The thresholds for TG-Ab and TPO-Ab for the Chinese population were established by big data analysis. PMID: 31476287 [PubMed - as supplied by publisher] (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - August 30, 2019 Category: Biochemistry Authors: Ma C, Li D, Yin Y, Wu J, Guo X, Zhang R, Hu Y, Zou Y, Li W, Wang D, Cheng X, Qiu L Tags: Clin Biochem Source Type: research

Neuroapoptosis in newborns with respiratory acidosis at birth.
CONCLUSIONS: Respiratory acidosis is associated to high concentrations of S100B protein in umbilical cord blood at birth. Umbilical cord blood pH and pCO2 may be useful in differentiating newborns at high risk of neuroapoptosis. Umbilical cord blood gas test may be valuable as risk indicator for neuroapoptosis at birth. PMID: 31473201 [PubMed - as supplied by publisher] (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - August 29, 2019 Category: Biochemistry Authors: Santotoribio JD, Cañavate-Solano C, Quintero-Prado R, González-Macías C, Soto-Pazos E, Sanchez ÁV, Mesa-Suárez P, Ramos-Ramos V, Cuadros-Muñoz JF, Mayor-Reyes M, Pérez-Ramos S, Fernández-Alba JJ Tags: Clin Biochem Source Type: research

Best practices in mitigating the risk of biotin interference with laboratory testing.
ran N Abstract Dietary biotin intake does not typically result in blood biotin concentrations that exceed interference thresholds for in vitro diagnostic tests. However, recent trends of high-dose biotin supplements and clinical trials of very high biotin doses for patients with multiple sclerosis have increased concerns about biotin interference with immunoassays. Estimates of the prevalence of high biotin intake vary, and patients may be unaware that they are taking biotin. Since 2016, 92 cases of suspected biotin interference have been reported to the US Food and Drug Administration. Immunoassays at greatest ri...
Source: Clinical Biochemistry - August 29, 2019 Category: Biochemistry Authors: Bowen R, Benavides R, Colón-Franco JM, Katzman BM, Muthukumar A, Sadrzadeh H, Straseski J, Klause U, Tran N Tags: Clin Biochem Source Type: research

Continuous reference intervals for 38 biochemical markers in healthy children and adolescents: Comparisons to traditionally partitioned reference intervals.
In this study, we establish biochemical marker continuous reference intervals for a Canadian population using healthy pediatric reference individuals and compare these to partitioned reference intervals. METHODS: Continuous reference intervals spanning 1-18.5 years of age were established using data from healthy CALIPER children and adolescents aged 6 months- 
Source: Clinical Biochemistry - August 22, 2019 Category: Biochemistry Authors: Asgari S, Higgins V, McCudden C, Adeli K Tags: Clin Biochem Source Type: research

Association between FSH, E1, and E2 levels in urine and serum in premenopausal and postmenopausal women.
CONCLUSION: The levels of FSH, E1 and E2 in urine correlated with those in the serum in premenopausal and postmenopausal women. Urine samples could be used instead of serum samples to measure hormone levels, which would reduce the difficulty of conducting large survey studies. PMID: 31442440 [PubMed - as supplied by publisher] (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - August 20, 2019 Category: Biochemistry Authors: Onizuka Y, Nagai K, Ideno Y, Kitahara Y, Iwase A, Yasui T, Nakajima-Shimada J, Hayashi K Tags: Clin Biochem Source Type: research

AD biomarker discovery in CSF and in alternative matrices.
Abstract Core Alzheimer's Disease (AD) biomarkers are the cerebrospinal fluid (CSF) proteins amyloid β42 and β40, and the tau proteins, total and phosphorylated. Their use is recommended by research guidelines for diagnostic purpose and for stratification of patients for clinical trials. However, novel potential biomarkers are needed, which can mirror risk factors and other different mechanisms of the disease, hopefully in less invasive biological fluids or matrices. Studies on blood, urine, saliva, tears gave promising results, and several novel molecules have been identified, as potential brain derived...
Source: Clinical Biochemistry - August 19, 2019 Category: Biochemistry Authors: Sancesario G, Bernardini S Tags: Clin Biochem Source Type: research

Rising above helium: A hydrogen carrier gas chromatography flame ionization detection (GC-FID) method for the simultaneous quantification of toxic alcohols and ethylene glycol in human plasma specimens.
Abstract Here we validate a GC, Flame Ionization Detection (GC-FID), liquid injection method using hydrogen as a carrier gas combining analysis of toxic volatile alcohols (VA): methanol, ethanol, isopropanol, acetone, as well as glycols, ethylene glycol (EG) and propylene glycol (PG), in a single method. METHODOLOGY: 200 μL of calibrator, QC, or patient specimen were deproteinized with 400 μL of acetonitrile containing internal standards (10 mmol/L N-propyl alcohol for VA and 2.5 mmol/L 1,2-butanediol for glycols). GC-FID analysis using hydrogen carrier gas and nitrogen makeup gas utilized an ...
Source: Clinical Biochemistry - August 16, 2019 Category: Biochemistry Authors: Buse J, Robinson JL, Shyne R, Chi Q, Affleck D, Duce D, Seiden-Long I Tags: Clin Biochem Source Type: research

Proteomics analysis of blood plasma in HIV-infected patients with chronic kidney disease.
CONCLUSION: The data suggests four proteins that may be used as biomarkers of CKD in HIV-infected patients. Further validation in a larger cohort of HIV-infected patients is necessary for assessing the clinical use of these proposed biomarkers for CKD. PMID: 31401122 [PubMed - as supplied by publisher] (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - August 8, 2019 Category: Biochemistry Authors: Lavinya AA, Lee CS, Hashim OH, Azwa I, Rajasuriar R, Lim SK, Wong YF Tags: Clin Biochem Source Type: research

Alzheimer's disease and other neurodegenerative dementias in comorbidity: A clinical and neuropathological overview.
Abstract Neuropathological diagnostic criteria of neurodegenerative disorders are based on the presence of specific inclusions in a specific area of brain tissue that correlate with clinical manifestations. Concomitant neurodegenerative disorders correspond to a combination of two (or more) different fully developed diseases in the same patient. Concomitant neurodegenerative pathology represents the presence of definite neurodegeneration and deposits of pathological proteins specific for another disease, which is not, however, fully developed. Very frequent overlaps include Alzheimer's disease and alpha-synuclein ...
Source: Clinical Biochemistry - August 7, 2019 Category: Biochemistry Authors: Matej R, Tesar A, Rusina R Tags: Clin Biochem Source Type: research

An investigation of enzymatic creatinine interference in a patient receiving dopamine and dobutamine.
CONCLUSION: Providers should be aware of erroneously low creatinine results when administering catecholamine drugs and collecting specimens through the same catheter. The mechanism of specimen contamination is consistent with a siphoning effect from one lumen to the other during collection with the infusion pumps still running. PMID: 31398309 [PubMed - as supplied by publisher] (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - August 6, 2019 Category: Biochemistry Authors: Seegmiller JC, Larson KA, Saenger AK Tags: Clin Biochem Source Type: research

Autoverification of test results in the core clinical laboratory.
Abstract Verification of laboratory test results represents the last opportunity to identify errors before they become part of the electronic medical record. Manual verification of test results places significant reliance on the experience and attentiveness of individual observers to identify errors and is vulnerable to errors through omission and neglect. Peer-reviewed publications have documented gains in process efficiency and quality improvement by use of middleware or laboratory information systems to autoverify test results based on pre-defined acceptability criteria. This review evaluates the acceptability ...
Source: Clinical Biochemistry - August 3, 2019 Category: Biochemistry Authors: Randell EW, Yenice S, Wamono AAK, Orth M Tags: Clin Biochem Source Type: research

HbA1c adjusted by erythrocyte creatine is a useful glycemic control indicator in patients with hemolysis.
CONCLUSIONS: These results suggested that ECadj-iA1c accurately reflected glycemic control in patients with hemolysis. PMID: 31386833 [PubMed - as supplied by publisher] (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - August 3, 2019 Category: Biochemistry Authors: Koga M, Inada S, Shibata M, Ijima H, Jinnouchi H, Ono Y, Iwasaka T, Tokuhiro S, Matsumura Y, Matsui H, Okumiya T Tags: Clin Biochem Source Type: research

CDKN2B gene expression is affected by 9p21.3 rs10757278 in CAD patients, six months after the MI.
CONCLUSIONS: We suggest a dysregulation of gene expression in the 9p21.3 region six months after acute MI, which is affected by a genetic variant in patients. The rs10757278 rare allele is one factor that might lead to prolonged risk for proatherogenic complications. PMID: 31386834 [PubMed - as supplied by publisher] (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - August 3, 2019 Category: Biochemistry Authors: Zivotić I, Djurić T, Stanković A, Milasinovic D, Stankovic G, Dekleva M, Nikolić NM, Alavantić D, Zivković M Tags: Clin Biochem Source Type: research

Playing the game of scientific publishing.
PMID: 31377346 [PubMed - as supplied by publisher] (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - August 1, 2019 Category: Biochemistry Authors: Fiala C, Diamandis EP Tags: Clin Biochem Source Type: research

High-sensitivity cardiac troponin testing during and after ACS: Complexed or not?
PMID: 31361993 [PubMed - as supplied by publisher] (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - July 27, 2019 Category: Biochemistry Authors: Hwang P, Mingels A, Kavsak PA Tags: Clin Biochem Source Type: research