Aspirin: bitter pill or miracle drug?
Abstract Acetylsalicylic acid (ASA) or brand name Aspirin is a widely available medication used to relieve inflammation, fever and pain. It has also been frequently prescribed as prevention for cardiovascular disease due to its anti-thrombotic qualities. However, ASA is also connected to increased internal bleeding, leading to concerns that this harmful side effect may outweigh its cardioprotective properties in some populations. In this review, we summarize data from several recent, large-scale clinical trials that put into the question the long-standing recommendations about prescribing ASA for primary cardiovas...
Source: Clinical Biochemistry - July 25, 2020 Category: Biochemistry Authors: Fiala C, Pasic MD Tags: Clin Biochem Source Type: research

Comparison of serologic and molecular SARS-CoV 2 results in a large cohort in Southern Tuscany demonstrates a role for serologic testing to increase diagnostic sensitivity.
Abstract BACKGROUND: Since February 2019, Italian hospitals registered COVID-19 (COronaVIrus Disease 19) cases more often than the rest of the country. During this epidemic, health authorities requested swab tests, whileseeking new patient paths. METHODS: A dual laboratory approach was evaluated, consisting ofpatient care reports for viral RNA detection on swabs and rapid serological tests in 516 patients (192 symptomatic or paucisymptomatic and 324 asymptomatic). RESULTS: We found the molecular positive fraction equal to 12% (23/192) among symptomatic/paucisymptomatic (S/P) and 15.4% (50/324) in asympto...
Source: Clinical Biochemistry - July 20, 2020 Category: Biochemistry Authors: Pancrazzi A, Magliocca P, Lorubbio M, Vaggelli G, Galano A, Mafucci M, Duranti D, Cortesi M, Mazzeschi E, Fabbroni S, Viti G, Tartaglia Polcini A, Tripodo E, Sanchini P, Gervino S, Tacconi D, Dei S, Mazzierli M, D'Urso A, Ognibene A Tags: Clin Biochem Source Type: research

Identification of plasma miR-106a-5p and miR-30a-5p as potential biomarkers for mesangial proliferative glomerulonephritis.
CONCLUSION: Plasma miR-30a-5p and miR-106a-5p can serve as novel and potential diagnostic biomarkers for MsPGN. PMID: 32673627 [PubMed - as supplied by publisher] (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - July 13, 2020 Category: Biochemistry Authors: Wang L, Lin J, Yu T, Zuo Q, Shen B, Zhang H, Liu B, Cai D, Mao H, Zhao H, Zou Q, Xiao B Tags: Clin Biochem Source Type: research

Real-world big-data studies in laboratory medicine: current status, application, and future considerations.
Abstract With the recent developments in information technology, real world big data studies (RWBDSs) have attracted increasing attention in the field of medicine. In RWBDSs, clinical laboratory data is an important part of the wider scope of real-world medical data, and its standardized use is critical for the generation of high-quality real-world evidence. To improve the core functioning and competitiveness of clinical laboratories as well as provide high-quality medical services for patients, it is important to construct an information analysis model and perform RWBDSs. However, among the majority of developing...
Source: Clinical Biochemistry - July 8, 2020 Category: Biochemistry Authors: Ma C, Wang X, Wu J, Cheng X, Xia L, Xue F, Qiu L Tags: Clin Biochem Source Type: research

Can Fecal Calprotectin Serve as a Screen for Necrotizing Enterocolitis in Infants?
Abstract BACKGROUND: Researchers are debating if fecal calprotectin results are useful for infant patients, especially in screening for necrotizing enterocolitis (NEC). Currently, none of the FDA-approved calprotectin assays provide cut-offs for infant patients. We retrospectively analyzed data from a reference laboratory and university hospital to investigate if cut-offs could be established for infant patients. METHODS: Data from a national reference laboratory of 5,144 test results for fecal calprotectin were analyzed for infant patients, and a cut-off was estimated based on the distribution of results. Ad...
Source: Clinical Biochemistry - July 3, 2020 Category: Biochemistry Authors: Goold E, Pearson L, Johnson LM Tags: Clin Biochem Source Type: research

Room-temperature-storable PCR Mixes for SARS-CoV-2 Detection.
CONCLUSION: The uptake of this approach will ease logistical challenges faced by transport networks and make more cold storage space available at diagnosis and hospital laboratories. PMID: 32592724 [PubMed - as supplied by publisher] (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - June 24, 2020 Category: Biochemistry Authors: Xu J, Wang J, Zhong Z, Su X, Yang K, Chen Z, Zhang D, Li T, Wang Y, Zhang S, Ge S, Zhang J, Xia N Tags: Clin Biochem Source Type: research

Omics insights into cancer histology; metabolomics and proteomics approach.
Abstract Metabolomics as a post-genomic research area comprising different analytical methods for small molecules analysis. One of the underlying applications of metabolomics technology for better disease diagnosis and prognosis is discovering the metabolic pathway differences between healthy individuals and patients. On the other hand, the other noteworthy applications of metabolomics include its effective role in biomarker screening for cancer detection, monitoring, and prediction. In other words, emerging of the metabolomics field can be hopeful to provide a suitable alternative for the common current cancer di...
Source: Clinical Biochemistry - June 23, 2020 Category: Biochemistry Authors: Tayanloo-Beik A, Sarvari M, Payab M, Gilany K, Alavi-Moghadam S, Gholami M, Goodarzi P, Larijani B, Arjmand B Tags: Clin Biochem Source Type: research

Response to 'ROC can be used to determine the optimal threshold of ALT for reflex to AST testing'.
PMID: 32579970 [PubMed - as supplied by publisher] (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - June 21, 2020 Category: Biochemistry Authors: Mohammed-Ali Z, Selvaratnam R Tags: Clin Biochem Source Type: research

Clinical and biochemical characteristics of patients with ornithine transcarbamylase deficiency.
CONCLUSION: unique profiles were observed in each group of OTCD patients, indicating specific physiological changes that happened to them. PMID: 32569589 [PubMed - as supplied by publisher] (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - June 19, 2020 Category: Biochemistry Authors: Peng MZ, Li XZ, Mei HF, Sheng HY, Yin X, Jiang MY, Cai YN, Su L, Lin YT, Shao YX, Liu L Tags: Clin Biochem Source Type: research

Usefulness of Estimated Average Glucose (eAG) in Glycemic Control and Cardiovascular Risk Reduction.
Abstract OBJECTIVE: One of the 8 regional health authority (RHA) zones in New Brunswick, Canada has implemented eAG since 2010. We sought to evaluate the clinical outcomes of glycemic control and cardiovascular risk levels before and after the eAG implementation in this zone; and to compare the overall outcomes of this zone with other 7 zones of the province. METHODS: Data (838,407 HbA1c values and 612,314 LDL-c values) was extracted from all adult diabetic patients in the provincial Diabetes Registry from 2008 to 2014. The Kruskal-Wallis statistic was conducted to compare the medians and inter quartile range...
Source: Clinical Biochemistry - June 14, 2020 Category: Biochemistry Authors: Wang D, Chen Y Tags: Clin Biochem Source Type: research

A novel COQ8A missense variant associated with a mild form of primary coenzyme Q10 deficiency type 4.
CONCLUSION: The p.Gln279Pro was detected in the KxGQ motif and the QKE triplet of the COQ8A protein, whose structures were crucial for the structure and function of the COQ8A protein associated with the biosynthesis of coenzyme Q10 and the proband's clinical symptoms were relatively milder than those previously reported. PMID: 32553579 [PubMed - as supplied by publisher] (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - June 14, 2020 Category: Biochemistry Authors: Liu G, Ma D, Li J, Luo C, Sun Y, Zhang J, Hu P, Tang W, Xu Z Tags: Clin Biochem Source Type: research

Shrunken pore syndrome - a common kidney disorder with high mortality. Diagnosis, prevalence, pathophysiology and treatment options.
Abstract Invasive studies show that the glomerular sieving coefficients for 5-30 kDa plasma proteins in the human kidney may be selectively reduced compared to those for small molecules
Source: Clinical Biochemistry - June 13, 2020 Category: Biochemistry Authors: Grubb A Tags: Clin Biochem Source Type: research

The potential of liquid biopsies in gastrointestinal cancer.
This article summarized the recent advances in liquid biopsy with a focus on novel technologies and the use of it in the screening, monitoring, and treatment of human GI cancer. CONTENT: The concept of liquid biopsy was first used to define the detection of circulating tumor cells (CTCs) in cancer patients, and has been expanded to other biomarkers in blood and body fluids, such as circulating tumor DNA (ctDNA), extracellular vesicles (EVs) and circulating tumor RNA. If analyzed with proper and advanced techniques like next generation sequencing (NGS) or proteomics, liquid biopsies can open an enormous array of potent...
Source: Clinical Biochemistry - June 12, 2020 Category: Biochemistry Authors: Wu C, Zhang J, Li H, Xu W, Zhang X Tags: Clin Biochem Source Type: research

Women in sports: the applicability of reference intervals for 6 commercially available testosterone immunoassays (HemSter Study).
ic AM Abstract BACKGROUND: Testosterone levels in female athletes are increased due to their physical activity and correlate with their exercise volume. We therefore hypothesized that the reference intervals (RIs) derived from the general population are not applicable for female athletes. The aim of this study was to evaluate the applicability of the given RIs for 6 commercially available testosterone immunoassays in a group of female athletes. METHODS: Our study included 121 female athletes from various sporting disciplines (water polo, handball, volleyball, football, and basketball). The physical activity s...
Source: Clinical Biochemistry - June 10, 2020 Category: Biochemistry Authors: Biljak VR, Đuras A, Čičak H, Vidranski V, Vidranski T, Peersman N, Vermeersch P, Ostroški I, Ružić L, Simundic AM Tags: Clin Biochem Source Type: research

Serum expression and diagnostic potential of long non-coding RNAs NEAT1 and TUG1 in viral hepatitis C and viral hepatitis C-associated hepatocellular carcinoma.
CONCLUSION: Significant alterations in the serum expression of NEAT1 and TUG1 in HCV and HCV-associated HCC patients were recorded. We propose NEAT1 and TUG1 as non-invasive, cost-effective and complementary biomarkers that improve the diagnostic characteristics of AFP. PMID: 32526227 [PubMed - as supplied by publisher] (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - June 8, 2020 Category: Biochemistry Authors: Mohyeldeen M, Ibrahim S, Shaker O, Helmy H Tags: Clin Biochem Source Type: research

Plasma RNA sequencing of extracellular RNAs reveals potential biomarkers for non-small cell lung cancer.
CONCLUSION: exRNA-seq from a small amount (400-500 μl) of plasma opens new possibilities for exploring lung cancer biomarkers in the plasma. PMID: 32526228 [PubMed - as supplied by publisher] (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - June 8, 2020 Category: Biochemistry Authors: Wang L, Wang J, Jia E, Liu Z, Ge Q, Zhao X Tags: Clin Biochem Source Type: research

Mass spectrometry.
PMID: 32511963 [PubMed - as supplied by publisher] (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - June 5, 2020 Category: Biochemistry Authors: Jannetto PJ, Danso D Tags: Clin Biochem Source Type: research

Pathophysiology and Significance of Natriuretic Peptides in Patients with End-stage Kidney Disease.
Abstract Natriuretic peptides (NP), especially B type (BNP) and its N-terminal pro-B type natriuretic peptide (NT-proBNP), have long been regarded as biomarkers of volume overload and tools to exclude heart failure in the general population. However, their role in end-stage kidney disease (ESKD) is less certain given that BNP and NT-proBNP are excreted by the kidney and so serum concentrations of NPs are nearly universally elevated compared to controls. Nevertheless, the accumulated evidence suggests thatserum concentrations of NPs in patients with ESKD show moderate or strong positive relationships with underlyin...
Source: Clinical Biochemistry - June 5, 2020 Category: Biochemistry Authors: Yang WL, Fahim M, Johnson DW Tags: Clin Biochem Source Type: research

Precision of autoantibody assays in Clinical Diagnostic Laboratories: what is the reality?
CONCLUSION: Our results allow proposing some acceptability limits for the precision performances of the autoantibody assays, compatible with the reality of life in diagnostic laboratories and clinical care. PMID: 32505738 [PubMed - as supplied by publisher] (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - June 4, 2020 Category: Biochemistry Authors: Senant M, Musset L, Chyderiotis G, Guis-Cabanne L, Damoiseaux J, Fabien N, Dragon-Durey MA Tags: Clin Biochem Source Type: research

Elevated plasma levels of asymmetric dimethylarginine and the risk for arrhythmic death in ischemic and non-ischemic, dilated cardiomyopathy - a prospective, controlled long-term study.
Abstract INTRODUCTION: Elevated plasma levels of asymmetric dimethylarginine (ADMA), an inhibitor of NO synthase, are associated with adverse outcome. There is no data available, whether ADMA levels are associated with arrhythmic death (AD) in patients with ischemic cardiomyopathy (ICM) or non-ischemic, dilated cardiomyopathy (DCM). METHODS AND RESULTS: A total of 110 ICM, 52 DCM and 30 control patients were included. Primary outcome parameter of this prospective study was arrhythmic death (AD) or resuscitated cardiac arrest (RCA). Plasma levels of ADMA were significantly higher in ICM (p
Source: Clinical Biochemistry - June 3, 2020 Category: Biochemistry Authors: Leo Burger A, Stojkovic S, Diedrich A, Demyanets S, Wojta J, Pezawas T Tags: Clin Biochem Source Type: research

Soluble urokinase plasminogen activator receptor (suPAR) is lower in disease-free patients but cannot rule out incident disease in patients with suspected cancer.
Abstract BACKGROUND: Accurate first-line diagnostics are essential for early recognition of cancer but also to identify patients free of disease. The biomarker soluble urokinase plasminogen activator receptor (suPAR) is elevated in patients with cancer or non-malignant disease compared to disease-free patients. We tested if low suPAR could be used to identify disease-free patients in an accelerated cancer diagnostics program, including ruling out cancer. METHODS: Patients with serious nonspecific symptoms and signs of cancer (NSSC) were included at the Diagnostic Outpatient Clinic, Copenhagen University Hospi...
Source: Clinical Biochemistry - June 3, 2020 Category: Biochemistry Authors: J H Rasmussen L, Schultz M, Iversen K, Eugen-Olsen J, Helms M, David K, Kjaer A, Lebech AM, Kronborg G Tags: Clin Biochem Source Type: research

Increased levels of BPI-ANCA in patients with primary Sj ögren's Syndrome are associated with lung involvement.
Increased levels of BPI-ANCA in patients with primary Sjögren's Syndrome are associated with lung involvement. Clin Biochem. 2020 Jun 02;: Authors: Wu Y, Yang Y, Qin Y, Wang Y, Feng M, Zhao X, Liang Z, Zhang C, Liu G, Guo H, Luo J Abstract BACKGROUND: The goal of this study was to investigate the association between bactericidal permeability increasing (BPI)-antineutrophil cytoplasmic antibody (ANCA) protein levels and primary Sjogren's syndrome (pSS) with lung involvement, as well as the potential diagnostic performance of BPI-ANCA. METHODS: The levels of BPI-ANCA in pSS patients with (n = ...
Source: Clinical Biochemistry - June 2, 2020 Category: Biochemistry Authors: Wu Y, Yang Y, Qin Y, Wang Y, Feng M, Zhao X, Liang Z, Zhang C, Liu G, Guo H, Luo J Tags: Clin Biochem Source Type: research

Analytical and Clinical Characterization of a Novel High-Sensitivity Cardiac Troponin Assay in a United States Population.
CONCLUSION: Analytical and clinical performance of the ACCESS hsTnI assay meets the definition of a hs cTn method. The ACCESS hsTnI assay has good precision over a wide range, no significant interferences, and sensitivity>90% and NPV≥99%. Performance is appropriate for aiding in AMI diagnosis. BACKGROUND: Approximately 7 million people in the United States present to emergency departments (ED) each year with signs and symptoms suggestive of the acute coronary syndromes (ACS), a continuum of cardiac ischemia that spans from stable angina to acute myocardial infarction (AMI). Of these, about 20% of patients (1.3 m...
Source: Clinical Biochemistry - May 30, 2020 Category: Biochemistry Authors: Christenson RH, Duh SH, Mullins KE, LeClair MM, Grigorov IL, Frank Peacock W Tags: Clin Biochem Source Type: research

ROC can be used to determine the optimal threshold of ALT for reflex to AST testing.
PMID: 32485170 [PubMed - as supplied by publisher] (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - May 30, 2020 Category: Biochemistry Authors: Yang CQ, Zhang GM Tags: Clin Biochem Source Type: research

A pharmacokinetic study to guide dosing of tenofovir disoproxil fumarate during different modalities of renal replacement therapy.
PMID: 32485171 [PubMed - as supplied by publisher] (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - May 30, 2020 Category: Biochemistry Authors: Dams K, Burger DM, Weyler J, Francque S, Jorens PG, Vanwolleghem T Tags: Clin Biochem Source Type: research

Laboratory Abnormalities in Children with Mild and Severe Coronavirus Disease 2019 (COVID-19): a pooled analysis and review.
Abstract Limited data exists to-date on the laboratory findings in children with COVID-19, warranting the conduction of this study, in which we pool the currently available literature data on the laboratory findings seen in children with mild and severe COVID-19. Following an extensive literature search, we identified 24 eligible studies, including a total of 624 pediatric cases with laboratory-confirmed COVID-19, which report data on 27 different biomarkers. We then performed a meta-analysis to calculate the pooled prevalence estimates (PPE) for these laboratory abnormalities in mild COVID-19. As data was too lim...
Source: Clinical Biochemistry - May 27, 2020 Category: Biochemistry Authors: Michael Henry B, Benoit SW, Helena Santos de Oliveira M, Chin Hsieh W, Benoit J, Ballout RA, Plebani M, Lippi G Tags: Clin Biochem Source Type: research

Prevalence of detectable biotin in The Netherlands in relation to risk on immunoassay interference.
In this study, we investigated biotin prevalence in The Netherlands in a representative cohort of routine laboratory requests in our laboratory using an LC-MS/MS assay for quantification of biotin in human plasma. In our study, we found 0.2% of samples exceeding 10 ng/mL of biotin, a finding more or less in line with the Australian data. Even though the biotin prevalence appears to be low, with concomitant low to moderate biotin concentrations, it is by no means a rare phenomenon. Laboratories like ours are likely to experience biotin positive samples on a daily basis with variable impact on patient care depending on the a...
Source: Clinical Biochemistry - May 27, 2020 Category: Biochemistry Authors: IJpelaar A, Beijers A, van Daal H, van den Ouweland JMW Tags: Clin Biochem Source Type: research

White blood cell count and total protein concentration to predict the absence of microcrystals in synovial fluid.
This study aimed to evaluate the predictive value of synovial fluid WBC count and total protein concentration for the absence of microcrystals. MATERIALS AND METHODS: This prospective study analyzed all synovial fluid samples collected at a single center in a 6-month period. The absolute WBC count and total protein concentration were recorded for each sample. A single expert used polarized light microscopy to detect microcrystals. Mann-Whitney U-tests was used to compare mean counts and concentrations in samples with and without crystals. Diagnostic performance was assessed through the area under the receiver-operatin...
Source: Clinical Biochemistry - May 26, 2020 Category: Biochemistry Authors: Aliste-Fernández M, San-José P, Aguadero V Tags: Clin Biochem Source Type: research

Upper Reference limits and percent measurable concentrations using a universal sample bank for high sensitivity cardiac troponin I using a point-of-care assay.
PMID: 32461099 [PubMed - as supplied by publisher] (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - May 24, 2020 Category: Biochemistry Authors: Wu AHB, Lynch K, Sexter A, Apple FS Tags: Clin Biochem Source Type: research

The effect of laboratory requisition modification, audit and feedback with academic detailing or both on utilization of blood urea testing in family practice in Newfoundland, Canada.
CONCLUSION: We conclude that removing urea from standard test order menus was the most effective in reducing test volumes, but combination with audit and feedback augmented the impact. PMID: 32450078 [PubMed - as supplied by publisher] (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - May 22, 2020 Category: Biochemistry Authors: Barrett BJ, Randell EW, Mariathas HH, Mohammadi A, Darcy S, Wilson R, Brian Johnston K, Parfrey PS Tags: Clin Biochem Source Type: research

Influence of sample centrifugation on plasma platelet count and activated partial thromboplastin time using patient samples.
Abstract BACKGROUND: Diagnostic coagulation testing is vulnerable to factors of the pre-analytical phase such as sample centrifugation. Despite this, centrifugation conditions differ widely among European laboratories. Here we use samples from patients referred for Activated partial thromboplastin time (APTT) testing to investigate if different centrifugation conditions result in platelet-poor plasma (PPP) (plasma platelet count
Source: Clinical Biochemistry - May 21, 2020 Category: Biochemistry Authors: Jesting A, Kemp Jacobsen K, De Cock A, De Preester H, Overgaard Foss Jensen K, Frank Joergensen S Tags: Clin Biochem Source Type: research

Proteomic Applications in Pathology and Laboratory Medicine: Present State and Future Prospects.
Abstract Clinical mass spectrometry applications have traditionally focused on small molecules, particularly in the areas of therapeutic drug monitoring, toxicology, and measurement of endogenous and exogenous steroids. More recently, the use of matrix assisted laser desorption/ionization time of flight mass spectrometry for the identification of microbial pathogens has been widely implemented. Following this evolution, there has been an expanding role for the measurement of peptides and proteins in pathology and laboratory medicine. This review explores the current state of protein measurement by clinical mass sp...
Source: Clinical Biochemistry - May 19, 2020 Category: Biochemistry Authors: Holmes DT, Romney MG, Angel P, DeMarco ML Tags: Clin Biochem Source Type: research

Uncarboxylated Matrix Gla-Protein: A Biomarker of Vitamin K Status and Cardiovascular Risk.
Abstract BACKGROUND: Dephosphorylated uncarboxylated matrix Gla-protein (dp-ucMGP) is a biomarker of functional vitamin K status. High plasma dp-ucMGP concentrations reflect a low vitamin K status and have been related to vascular calcification. Our aims were to assess plasma levels of dp-ucMGP and their association with cardiovascular risk in a general population. METHODS: Plasma dp-ucMGP measurements were performed using the IDS-iSYS InaKtif MGP assay in 491 consecutive participants in a Danish general population study (229 males and 262 females, aged 19-71 years). Multivariable linear and logistic regressi...
Source: Clinical Biochemistry - May 15, 2020 Category: Biochemistry Authors: T J, Lt M, Bh T, T S, P R, U T, Nr J, Bl C, J J, M FM, A L Tags: Clin Biochem Source Type: research

Reducing free thyroid hormone testing through multiple Plan-Do-Study-Act cycles.
CONCLUSIONS: To achieve optimal reductions in free thyroid hormone testing, multiple PDSA cycles were required alongside assessing process and balancing measures. Overall, fT4 and fT3 testing was decreased by 39% and 47%, respectively. PMID: 32416171 [PubMed - as supplied by publisher] (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - May 13, 2020 Category: Biochemistry Authors: Taher J, Beriault DR, Yip D, Tahir S, Hicks LK, Gilmour JA Tags: Clin Biochem Source Type: research

Lipid profile and lipid oxidative modification parameters in the first trimester of high- risk pregnancies - possibilities for preeclampsia prediction.
Abstract OBJECTIVES: The goal of this study was to investigate metabolic changes in lipids and oxidative stress parameters in the first trimester of pregnancy with the more specific aim of estimating the significance and strength of researched parameters in the prediction of preeclampsia. Design and Methods The study included 87 high-risk pregnant (HRG) female subjects, 14 with developed preeclampsia (PEC) and 43 healthy pregnant female subjects matched for gestational age (CG). Thiobarbituric acid-reactive substances (TBARS) concentration, lipid hydroperoxides (LOOH), pro-oxidant antioxidant balance (PAB) and tot...
Source: Clinical Biochemistry - May 11, 2020 Category: Biochemistry Authors: Ardalić D, Stefanović A, Banjac G, Cabunac P, Miljkovic M, Mandić-Marković V, Stanimirović S, Damnjanović Pažin B, Spasić S, Spasojević-Kalimanovska V, Karadžov-Orlić N, Miković Z Tags: Clin Biochem Source Type: research

Method validation for a multi-element panel in serum by inductively coupled plasma mass spectrometry (ICP-MS).
Abstract BACKGROUND: Laboratory testing for trace and toxic elements is important to diagnose metal toxicity and nutritional deficiency. There are several essential elements that are necessary for biological function and non-essential elements that can pose risk from exposure. Both essential and nonessential elements can be toxic if concentrations exceed a certain threshold. METHODS: An aliquot of serum was diluted in a diluent solution, which contained iridium (Ir) as the internal standard, gold (Au), 0.05% Triton X-100, and 1% nitric acid (HNO3). The diluted specimen was aspirated into an inductively couple...
Source: Clinical Biochemistry - May 11, 2020 Category: Biochemistry Authors: Johnson-Davis KL, Farnsworth C, Law C, Parker R Tags: Clin Biochem Source Type: research

Alpha-1-antitrypsin molecular testing in Canada: A seven year, multi-centre comparison.
Abstract BACKGROUND: Laboratory confirmation of alpha-1-antitrypsin (A1AT) deficiency may be achieved by multiple methods. Here, we compare the relative comprehensiveness and efficiency of pathogenic variant (PV) detection of four different protocols utilized at different diagnostic centres in Canada. METHODS: Diagnostic results from 2011 to 2018 at clinical laboratories in British Columbia (BC), Alberta (AB), Ontario (ON), and Québec (QC) were reviewed. The four labs utilize the following protocols: BC-CGID (serum A1AT Concentration/Genotyping/Isoelectric focussing (IEF)/SERPINA1 DNA sequencing), AB-C...
Source: Clinical Biochemistry - May 6, 2020 Category: Biochemistry Authors: Mattman A, Gilfix BM, Chen SX, DeMarco ML, Kyle BD, Parker ML, Agbor TA, Jung B, Selvarajah S, Barakauskas VE, Vaags AK, Estey MP, Nelson TN, Speevak MD Tags: Clin Biochem Source Type: research

Glucose 6 Phosphate Dehydrogenase Deficiency: Description of a novel and de novo variant associated with chronic nonspherocytic hemolytic anemia.
PMID: 32387441 [PubMed - as supplied by publisher] (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - May 6, 2020 Category: Biochemistry Authors: Feliu Torres A Tags: Clin Biochem Source Type: research

Changes in redox state of albumin before and after kidney transplantation in patients with end-stage renal disease.
CONCLUSIONS: Actual reduced albumin levels were found to exceed pre-transplant levels on or after day 30 following kidney transplantation unlike immediate restoration of renal function. Renal function was recovered immediately following kidney transplantation, but reduced albumen concentration increased above the pre-transplant levels only from day 30 after transplantation. PMID: 32380091 [PubMed - as supplied by publisher] (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - May 4, 2020 Category: Biochemistry Authors: Tanaka R, Imafuku T, Suzuki Y, Nishida K, Matsusaka K, Shin T, Sato Y, Ishima Y, Watanabe H, Mimata H, Maruyama T, Itoh H Tags: Clin Biochem Source Type: research

How to write (and how not to write) a scientific review article.
PMID: 32371050 [PubMed - as supplied by publisher] (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - May 1, 2020 Category: Biochemistry Authors: Avram Sanders D Tags: Clin Biochem Source Type: research

Emerging key laboratory tests for patients with COVID-19.
PMID: 32360478 [PubMed - as supplied by publisher] (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - April 30, 2020 Category: Biochemistry Authors: Kavsak PA, de Wit K, Worster A Tags: Clin Biochem Source Type: research

2019 novel coronavirus disease (COVID-19) in hemodialysis patients: a report of two cases.
CONCLUSION: Two COVID-19 patients on maintenance hemodialysis discharged after a month of hospitalization. The removal of cytokines through blood purification technology may be beneficial for the recovery of COVID-19 patients. PMID: 32360479 [PubMed - as supplied by publisher] (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - April 30, 2020 Category: Biochemistry Authors: Ke C, Wang Y, Zeng X, Yang C, Hu Z Tags: Clin Biochem Source Type: research

Evaluation of complement system proteins C3a, C5a and C6 in patients of endometriosis.
Abstract BACKGROUND: Endometriosis is a disease that shows auto-immune and chronic characteristics, suggesting a role for proteins mediating immune interactions in its pathophysiology. The aim was to evaluate C3a and C5a for their role in inflammatory responses and C6 as the down-stream interactor following our previous findings on C5 mRNA expression changes in endometriosis [1]. METHODS: Sera from 71 endometriosis patients and 77 women without endometriosis were taken. While the samples were taken only once from the controls, the patient samples were taken before, in 1st and in 7th days after laparoscopy. Le...
Source: Clinical Biochemistry - April 20, 2020 Category: Biochemistry Authors: Karadadaş E, Hortu İ, Ak H, Mete Ergenoğlu A, Karadadaş N, Hakan Aydın H Tags: Clin Biochem Source Type: research

Evaluation of Plasma ACTH Stability using the Roche Elecsys Immunoassay.
Abstract BACKGROUND: Adrenocorticotropic hormone (ACTH) has been reported to be labile in blood due to proteolytic degradation and stringent procedures are followed to prevent in vitro degradation after sample collection. OBJECTIVE: The purpose of this study was to examine the effect of time and temperature before and after separation of plasma from cells in the quantitation of plasma ACTH. METHODS: Our current protocol includes sample collection in a pre-chilled tube, transport on ice and immediate centrifugation at 4°C. These reference conditions were compared against sample processing in tubes and...
Source: Clinical Biochemistry - April 18, 2020 Category: Biochemistry Authors: Nandakumar V, Paul Theobald J, Algeciras-Schimnich A Tags: Clin Biochem Source Type: research

Analysis of neutrophil gelatinase-associated lipocalin, vascular endothelial growth factor, and soluble receptor for advanced glycation end-products in bone marrow supernatant in hematologic malignancies.
CONCLUSION: NGAL was related to neutrophil count and VEGF. NGAL and VEGF showed similar intergroup patterns, reflecting that NGAL was associated with VEGF. PMID: 32304694 [PubMed - as supplied by publisher] (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - April 15, 2020 Category: Biochemistry Authors: Cho CH, Cha J Tags: Clin Biochem Source Type: research

Clinical evaluation of Ortho Clinical Diagnostics high-sensitivity cardiac troponin I assay in patients with symptoms suggestive of acute coronary syndrome.
Abstract BACKGROUND: As more companies obtain regulatory approval for high-sensitivity cardiac troponin (hs-cTn) assays there is an urgent need for independent analytical and clinical evaluations. To this end, we have evaluated Ortho Clinical Diagnostics' hs-cTnI assay and compared it to their contemporary cTnI-ES assay in emergency department (ED) patients with suspected acute coronary syndrome (ACS). METHODS: The study cohort consisted of ED patients (n=906) with symptoms suggestive of ACS who had Ortho hs-cTnI and cTnI-ES results at presentation and 3h (with calculated delta(0-3h) defined as the absolute c...
Source: Clinical Biochemistry - April 15, 2020 Category: Biochemistry Authors: Kavsak PA, Mondoux SE, Sherbino J, Ma J, Clayton N, Hill SA, McQueen M, Mehta SR, Griffith LE, Devereaux PJ, Worster A Tags: Clin Biochem Source Type: research

Establishing reference intervals for major biochemical analytes for the Russian population: a research conducted as a part of the IFCC global study on reference values.
CONCLUSION: RIs matched to the Russian population were established for 34 biochemical analytes using up-to-date methods in detailed consideration of sources of variation of RVs. The majority of Russian RIs are similar to those of Caucasian populations among the participating countries. PMID: 32278594 [PubMed - as supplied by publisher] (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - April 9, 2020 Category: Biochemistry Authors: Evgina S, Ichihara K, Ruzhanskaya A, Skibo I, Vybornova N, Vasiliev A, Kimura S, Butlitski D, Volkova E, Vilenskaya E, Emanuel V Tags: Clin Biochem Source Type: research

Relationship between biological variation and delta check rules performance.
CONCLUSIONS: The difference in within-subject biological variation in different patients may contribute to the observed discrepancy in predicted and empirical delta check performance. PMID: 32247779 [PubMed - as supplied by publisher] (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - April 2, 2020 Category: Biochemistry Authors: Zhen Tan R, Markus C, Ping Loh T Tags: Clin Biochem Source Type: research

Reference change values based on uncertainty models.
CONCLUSIONS: The estimation of reference change values using our models could be more realistic than the classical approaches because more identified variation sources were considered. We hope that this study will help and stimulate clinical laboratories to perform uncertainty and reference change values studies, and it allows a greater understanding of these concepts and its application in disease monitoring. PMID: 32243886 [PubMed - as supplied by publisher] (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - March 31, 2020 Category: Biochemistry Authors: Rigo-Bonnin R, Muñoz-Provencio D, Canalias F Tags: Clin Biochem Source Type: research

Quality over Quantity: A Qualitative, Targeted Bottom-up Proteomics Approach to Genotyping Apolipoprotein L1.
Abstract A targeted, bottom-up proteomic assay was developed for the qualitative detection of apolipoprotein L1 (ApoL1) protein variants (G0, G1, and G2) in blood plasma for identification of high and low renal risk genotypes. Following trypsin digestion of liquid or dry plasma, surrogate peptides specific to each ApoL1 variant were detected by liquid chromatography-tandem mass spectrometry along with two surrogate peptides common among all variants which served as internal (positive) controls to verify correct sample processing. Using isotopically labeled peptide internal standards, the presence or absence of eac...
Source: Clinical Biochemistry - March 28, 2020 Category: Biochemistry Authors: Norris Bradley M, Shufor CM, Holland PL, Levandoski M, Grant RP Tags: Clin Biochem Source Type: research