Pediatric reference intervals for 32 routine biochemical markers using the siemens healthineers atellica ® CH assays in healthy children and adolescents
CONCLUSION: The current study expands the clinical utility of the CALIPER database to include 32 Siemens Atellica® chemistry assays. Reference value distributions for Siemens assays mirrored those observed on other comparable assays/systems with few exceptions (e.g. lipase, direct and total bilirubin). These finding support the robustness of previously derived reference intervals in the CALIPER cohort and other global cohorts.PMID:34687749 | DOI:10.1016/j.clinbiochem.2021.10.006 (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - October 23, 2021 Category: Biochemistry Authors: Mary Kathryn Bohn Paul Horn Donna League Paul Steele Alexandra Hall Khosrow Adeli Source Type: research

Small Volume Retinol Binding Protein Measurement by Liquid Chromatography-Tandem Mass Spectrometry
CONCLUSION: Liquid chromatography-tandem mass spectrometry facilitated testing a smaller sample volume, thereby increasing the ability to monitor key nutritional markers in premature infants. The differences in results compared with a commercially-available nephelometric assay revealed questionable results for lower concentrations by immunoassay.PMID:34678307 | DOI:10.1016/j.clinbiochem.2021.10.005 (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - October 22, 2021 Category: Biochemistry Authors: William S Phipps Dina N Greene Hannah Pflaum Thomas J Laha Jane A Dickerson Jill Irvine Anna E Merrill Pratistha Ranjitkar Clark M Henderson Andrew N Hoofnagle Source Type: research

Laboratory Biomarkers of Multiple Sclerosis (MS)
Clin Biochem. 2021 Oct 18:S0009-9120(21)00275-7. doi: 10.1016/j.clinbiochem.2021.10.004. Online ahead of print.ABSTRACTMultiple sclerosis (MS) is a neurological disease that affects the central nervous system (CNS). The diagnosis of the disease is quite challenging due to its variation among patients. As a result, the need to enhance diagnostic procedures, evaluate objective prognostic markers and promote effective monitoring of patients' responses to treatment has prompted the identification of many biomarkers. To present up-to-date knowledge on potential biomarkers for MS used to assess disease activity, progression, and...
Source: Clinical Biochemistry - October 21, 2021 Category: Biochemistry Authors: Borros Arneth J örg Kraus Source Type: research

Best practice in statistics: The use of log transformation
Ann Clin Biochem. 2021 Oct 19:45632211050531. doi: 10.1177/00045632211050531. Online ahead of print.ABSTRACTThe log transformation is often used to reduce skewness of a measurement variable. If, after transformation, the distribution is symmetric, then the Welch t-test might be used to compare groups. If, also, the distribution becomes close to normal, then a reference interval might be determined.PMID:34666549 | DOI:10.1177/00045632211050531 (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - October 20, 2021 Category: Biochemistry Authors: Robert M West Source Type: research

Best practice in statistics: The use of log transformation
Ann Clin Biochem. 2021 Oct 19:45632211050531. doi: 10.1177/00045632211050531. Online ahead of print.ABSTRACTThe log transformation is often used to reduce skewness of a measurement variable. If, after transformation, the distribution is symmetric, then the Welch t-test might be used to compare groups. If, also, the distribution becomes close to normal, then a reference interval might be determined.PMID:34666549 | DOI:10.1177/00045632211050531 (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - October 20, 2021 Category: Biochemistry Authors: Robert M West Source Type: research

Best practice in statistics: The use of log transformation
Ann Clin Biochem. 2021 Oct 19:45632211050531. doi: 10.1177/00045632211050531. Online ahead of print.ABSTRACTThe log transformation is often used to reduce skewness of a measurement variable. If, after transformation, the distribution is symmetric, then the Welch t-test might be used to compare groups. If, also, the distribution becomes close to normal, then a reference interval might be determined.PMID:34666549 | DOI:10.1177/00045632211050531 (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - October 20, 2021 Category: Biochemistry Authors: Robert M West Source Type: research

Best practice in statistics: The use of log transformation
Ann Clin Biochem. 2021 Oct 19:45632211050531. doi: 10.1177/00045632211050531. Online ahead of print.ABSTRACTThe log transformation is often used to reduce skewness of a measurement variable. If, after transformation, the distribution is symmetric, then the Welch t-test might be used to compare groups. If, also, the distribution becomes close to normal, then a reference interval might be determined.PMID:34666549 | DOI:10.1177/00045632211050531 (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - October 20, 2021 Category: Biochemistry Authors: Robert M West Source Type: research

Can patients with mild post-operative hyponatraemia following elective arthroplasty be discharged safely? A large-scale service evaluation suggests they can
CONCLUSION: Post-operative hyponatraemia is common and may be a marker of pre-operative vulnerability. In these cohorts, it was not independently associated with LOS, re-attendance or re-admission to hospital. We suggest that otherwise well patients with mild hyponatraemia can be safely discharged earlier than is often the case and may not require extensive investigation. Further examination and research is required to develop a pre-operative approach to predict which patients will develop significant post-operative hyponatraemia.PMID:34663082 | DOI:10.1177/00045632211051526 (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - October 19, 2021 Category: Biochemistry Authors: Jonathan Macdonald Emma Cunningham Nicola Gallagher Paul Hamilton Roslyn Cassidy Leeann Bryce David Beverland Source Type: research

Can patients with mild post-operative hyponatraemia following elective arthroplasty be discharged safely? A large-scale service evaluation suggests they can
CONCLUSION: Post-operative hyponatraemia is common and may be a marker of pre-operative vulnerability. In these cohorts, it was not independently associated with LOS, re-attendance or re-admission to hospital. We suggest that otherwise well patients with mild hyponatraemia can be safely discharged earlier than is often the case and may not require extensive investigation. Further examination and research is required to develop a pre-operative approach to predict which patients will develop significant post-operative hyponatraemia.PMID:34663082 | DOI:10.1177/00045632211051526 (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - October 19, 2021 Category: Biochemistry Authors: Jonathan Macdonald Emma Cunningham Nicola Gallagher Paul Hamilton Roslyn Cassidy Leeann Bryce David Beverland Source Type: research

Stability of maternal serum free β-hCG following whole blood sample transit: First trimester Down's syndrome screening in Scotland
CONCLUSION: The current sample receipt protocol in use by the Scottish Down's syndrome screening programme is fit for purpose.PMID:34657475 | DOI:10.1177/00045632211045250 (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - October 18, 2021 Category: Biochemistry Authors: Angela Ballantyne Lorna Rashid Rebecca Pattenden Source Type: research

Validating the NIH LDL-C Equation in a Specialized Lipid Cohort: Does it Add Up?
CONCLUSIONS: We validated the NIH equation against ultracentrifugation in a cohort with a wide lipid concentration range, which supported its superiority over the Friedewald equation. We recommend clinical implementing the NIH equation for all patients except those with type III hyperlipoproteinemia or TG>9.04 mmol/L, with an LDL-C lower reporting limit of
Source: Clinical Biochemistry - October 17, 2021 Category: Biochemistry Authors: Victoria Higgins Lawrence A Leiter Sarah R Delaney Daniel R Beriault Source Type: research

Validating the NIH LDL-C Equation in a Specialized Lipid Cohort: Does it Add Up?
CONCLUSIONS: We validated the NIH equation against ultracentrifugation in a cohort with a wide lipid concentration range, which supported its superiority over the Friedewald equation. We recommend clinical implementing the NIH equation for all patients except those with type III hyperlipoproteinemia or TG>9.04 mmol/L, with an LDL-C lower reporting limit of
Source: Clinical Biochemistry - October 17, 2021 Category: Biochemistry Authors: Victoria Higgins Lawrence A Leiter Sarah R Delaney Daniel R Beriault Source Type: research

UKMedLab2021 conference-building a new platform for the future
Ann Clin Biochem. 2021 Oct 13:45632211040679. doi: 10.1177/00045632211040679. Online ahead of print.NO ABSTRACTPMID:34645323 | DOI:10.1177/00045632211040679 (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - October 14, 2021 Category: Biochemistry Authors: Bernard Croal Sarah Robinson Source Type: research

UKMedLab2021 conference-building a new platform for the future
Ann Clin Biochem. 2021 Oct 13:45632211040679. doi: 10.1177/00045632211040679. Online ahead of print.NO ABSTRACTPMID:34645323 | DOI:10.1177/00045632211040679 (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - October 14, 2021 Category: Biochemistry Authors: Bernard Croal Sarah Robinson Source Type: research

UKMedLab2021 conference-building a new platform for the future
Ann Clin Biochem. 2021 Oct 13:45632211040679. doi: 10.1177/00045632211040679. Online ahead of print.NO ABSTRACTPMID:34645323 | DOI:10.1177/00045632211040679 (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - October 14, 2021 Category: Biochemistry Authors: Bernard Croal Sarah Robinson Source Type: research

Utility of P-SEP, sTREM-1 and suPAR as Novel Sepsis Biomarkers in SARS-CoV-2 Infection
Indian J Clin Biochem. 2021 Oct 6:1-8. doi: 10.1007/s12291-021-01008-6. Online ahead of print.ABSTRACTThe coronavirus disease 2019 is a highly contagious viral infection caused by SARS-CoV-2 virus, member of coronaviridae family. It causes life threatening complications due to complexity and rapid onset course of the disease. Early identification of high-risk patients who require close monitoring and aggressive treatment remains challengeable till date. Novel biomarkers which help to identify high risk patients at the early stage is high priority. Objective of this review to find utility of P-SEP, sTREM-1 and suPAR for dia...
Source: Clinical Biochemistry - October 13, 2021 Category: Biochemistry Authors: Sagar Dholariya Deepak N Parchwani Ragini Singh Madhuri Radadiya C D S Katoch Source Type: research

Utility of P-SEP, sTREM-1 and suPAR as Novel Sepsis Biomarkers in SARS-CoV-2 Infection
Indian J Clin Biochem. 2021 Oct 6:1-8. doi: 10.1007/s12291-021-01008-6. Online ahead of print.ABSTRACTThe coronavirus disease 2019 is a highly contagious viral infection caused by SARS-CoV-2 virus, member of coronaviridae family. It causes life threatening complications due to complexity and rapid onset course of the disease. Early identification of high-risk patients who require close monitoring and aggressive treatment remains challengeable till date. Novel biomarkers which help to identify high risk patients at the early stage is high priority. Objective of this review to find utility of P-SEP, sTREM-1 and suPAR for dia...
Source: Clinical Biochemistry - October 13, 2021 Category: Biochemistry Authors: Sagar Dholariya Deepak N Parchwani Ragini Singh Madhuri Radadiya C D S Katoch Source Type: research

Creatine kinase test diagnostic accuracy in neonatal screening for Duchenne Muscular Dystrophy: a systematic review
CONCLUSIONS: the creatine kinase test showed good accuracy in screening for cases of Duchenne Muscular Dystrophy and may be a useful alternative in the early diagnosis of the disease followed by confirmatory genetic testing.PMID:34626608 | DOI:10.1016/j.clinbiochem.2021.09.010 (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - October 9, 2021 Category: Biochemistry Authors: Kelli Carneiro de Freitas Nakata Priscilla Perez da Silva Pereira Bruno Salgado Riveros Source Type: research

Novel mutations associated with Carnitine-Acylcarnitine Translocase and Carnitine Palmitoyl Transferase 2 deficiencies in Malaysia
CONCLUSIONS: The use of the (C16+C18:1)/C2 acylcarnitine ratio in dried blood spot in our experience improves the diagnostic specificity for CACT/CPT2 deficiencies over long chain acylcarnitine (C16 and C18:1) alone. DNA sequencing for both genes aids in confirming the diagnosis.PMID:34626609 | DOI:10.1016/j.clinbiochem.2021.10.002 (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - October 9, 2021 Category: Biochemistry Authors: Anasufiza Habib Nor Azimah Abdul Azize Salina Abd Rahman Yusnita Yakob Vengadeshwaran Suberamaniam Muhammad Irfan Bukhari Ahmad Nazri Huzaimah Abdullah Sani Gaik-Siew Ch'ng Leong Huey Yin Simon Olpin Ngu Lock-Hock Source Type: research

Updated reference intervals for urine normetanephrine have no effect on test sensitivity but fewer false positives
CONCLUSIONS: Reference intervals for UNM derived from actual clinical population screening data are higher than in traditional healthy volunteers. Use of these more appropriate reference intervals can significantly reduce the false positive rate without compromising test sensitivity for true pheochromocytoma.PMID:34626610 | DOI:10.1016/j.clinbiochem.2021.09.013 (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - October 9, 2021 Category: Biochemistry Authors: Gregory A Kline Jessica Boyd Martin Hyrcza Daniele Pacaud Janice L Pasieka Yuan Dong Hossein Sm Sadrzadeh Alexander Ac Leung Source Type: research

Development of Nation-wide Reference Intervals Using an Indirect Method and Harmonized Assays
CONCLUSIONS: To our knowledge, this is the largest collection of RIs that were calculated by an indirect method representing clinical chemistry, endocrinology, coagulation, and hematology analytes that have been derived with very powerful "Ns" for each age bracket. This process provides more robust RIs and allows for the determination of pediatric and geriatric RIs that would otherwise be difficult to obtain using traditional direct RI determinations.PMID:34626611 | DOI:10.1016/j.clinbiochem.2021.10.001 (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - October 9, 2021 Category: Biochemistry Authors: James K Fleming Alex Katayev Candace M Moorer Denean A Ward-Jeffries Colon L Terrell Source Type: research

Creatine kinase test diagnostic accuracy in neonatal screening for Duchenne Muscular Dystrophy: a systematic review
CONCLUSIONS: the creatine kinase test showed good accuracy in screening for cases of Duchenne Muscular Dystrophy and may be a useful alternative in the early diagnosis of the disease followed by confirmatory genetic testing.PMID:34626608 | DOI:10.1016/j.clinbiochem.2021.09.010 (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - October 9, 2021 Category: Biochemistry Authors: Kelli Carneiro de Freitas Nakata Priscilla Perez da Silva Pereira Bruno Salgado Riveros Source Type: research

Novel mutations associated with Carnitine-Acylcarnitine Translocase and Carnitine Palmitoyl Transferase 2 deficiencies in Malaysia
CONCLUSIONS: The use of the (C16+C18:1)/C2 acylcarnitine ratio in dried blood spot in our experience improves the diagnostic specificity for CACT/CPT2 deficiencies over long chain acylcarnitine (C16 and C18:1) alone. DNA sequencing for both genes aids in confirming the diagnosis.PMID:34626609 | DOI:10.1016/j.clinbiochem.2021.10.002 (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - October 9, 2021 Category: Biochemistry Authors: Anasufiza Habib Nor Azimah Abdul Azize Salina Abd Rahman Yusnita Yakob Vengadeshwaran Suberamaniam Muhammad Irfan Bukhari Ahmad Nazri Huzaimah Abdullah Sani Gaik-Siew Ch'ng Leong Huey Yin Simon Olpin Ngu Lock-Hock Source Type: research

Updated reference intervals for urine normetanephrine have no effect on test sensitivity but fewer false positives
CONCLUSIONS: Reference intervals for UNM derived from actual clinical population screening data are higher than in traditional healthy volunteers. Use of these more appropriate reference intervals can significantly reduce the false positive rate without compromising test sensitivity for true pheochromocytoma.PMID:34626610 | DOI:10.1016/j.clinbiochem.2021.09.013 (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - October 9, 2021 Category: Biochemistry Authors: Gregory A Kline Jessica Boyd Martin Hyrcza Daniele Pacaud Janice L Pasieka Yuan Dong Hossein Sm Sadrzadeh Alexander Ac Leung Source Type: research

Development of Nation-wide Reference Intervals Using an Indirect Method and Harmonized Assays
CONCLUSIONS: To our knowledge, this is the largest collection of RIs that were calculated by an indirect method representing clinical chemistry, endocrinology, coagulation, and hematology analytes that have been derived with very powerful "Ns" for each age bracket. This process provides more robust RIs and allows for the determination of pediatric and geriatric RIs that would otherwise be difficult to obtain using traditional direct RI determinations.PMID:34626611 | DOI:10.1016/j.clinbiochem.2021.10.001 (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - October 9, 2021 Category: Biochemistry Authors: James K Fleming Alex Katayev Candace M Moorer Denean A Ward-Jeffries Colon L Terrell Source Type: research

Creatine kinase test diagnostic accuracy in neonatal screening for Duchenne Muscular Dystrophy: a systematic review
CONCLUSIONS: the creatine kinase test showed good accuracy in screening for cases of Duchenne Muscular Dystrophy and may be a useful alternative in the early diagnosis of the disease followed by confirmatory genetic testing.PMID:34626608 | DOI:10.1016/j.clinbiochem.2021.09.010 (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - October 9, 2021 Category: Biochemistry Authors: Kelli Carneiro de Freitas Nakata Priscilla Perez da Silva Pereira Bruno Salgado Riveros Source Type: research

Novel mutations associated with Carnitine-Acylcarnitine Translocase and Carnitine Palmitoyl Transferase 2 deficiencies in Malaysia
CONCLUSIONS: The use of the (C16+C18:1)/C2 acylcarnitine ratio in dried blood spot in our experience improves the diagnostic specificity for CACT/CPT2 deficiencies over long chain acylcarnitine (C16 and C18:1) alone. DNA sequencing for both genes aids in confirming the diagnosis.PMID:34626609 | DOI:10.1016/j.clinbiochem.2021.10.002 (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - October 9, 2021 Category: Biochemistry Authors: Anasufiza Habib Nor Azimah Abdul Azize Salina Abd Rahman Yusnita Yakob Vengadeshwaran Suberamaniam Muhammad Irfan Bukhari Ahmad Nazri Huzaimah Abdullah Sani Gaik-Siew Ch'ng Leong Huey Yin Simon Olpin Ngu Lock-Hock Source Type: research

Updated reference intervals for urine normetanephrine have no effect on test sensitivity but fewer false positives
CONCLUSIONS: Reference intervals for UNM derived from actual clinical population screening data are higher than in traditional healthy volunteers. Use of these more appropriate reference intervals can significantly reduce the false positive rate without compromising test sensitivity for true pheochromocytoma.PMID:34626610 | DOI:10.1016/j.clinbiochem.2021.09.013 (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - October 9, 2021 Category: Biochemistry Authors: Gregory A Kline Jessica Boyd Martin Hyrcza Daniele Pacaud Janice L Pasieka Yuan Dong Hossein Sm Sadrzadeh Alexander Ac Leung Source Type: research

Development of Nation-wide Reference Intervals Using an Indirect Method and Harmonized Assays
CONCLUSIONS: To our knowledge, this is the largest collection of RIs that were calculated by an indirect method representing clinical chemistry, endocrinology, coagulation, and hematology analytes that have been derived with very powerful "Ns" for each age bracket. This process provides more robust RIs and allows for the determination of pediatric and geriatric RIs that would otherwise be difficult to obtain using traditional direct RI determinations.PMID:34626611 | DOI:10.1016/j.clinbiochem.2021.10.001 (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - October 9, 2021 Category: Biochemistry Authors: James K Fleming Alex Katayev Candace M Moorer Denean A Ward-Jeffries Colon L Terrell Source Type: research

Serum arachidonic acid levels is a predictor of poor functional outcome in acute intracerebral hemorrhage
This study aimed to investigate the relationship between serum levels of PUFAs (including AA) and functional outcomes among intracerebral hemorrhage (ICH) patients.METHODS: From November 2012 to July 2020, ICH patients within 24 h from onset were enrolled. Patients were divided into a good functional outcome group (modified Rankin Scale [mRS] score at 3 months, 0-3) and a poor functional outcome group (mRS score at 3 months, 4-6). We compared baseline variables between groups.RESULTS: Participants comprised 133 patients (mean age, 60 years), with 106 patients (80%) in the good functional outcome group and 27 patients (20%)...
Source: Clinical Biochemistry - October 8, 2021 Category: Biochemistry Authors: Junichiro Takahashi Kenichiro Sakai Takeo Sato Hiroki Takatsu Teppei Komatsu Hidetaka Mitsumura Hidetomo Murakami Yasuyuki Iguchi Source Type: research

Biological variation of secretoneurin; a novel cardiovascular biomarker implicated in arrhythmogenesis
This study reports the within subject variation (CVI), between subject variation (CVG), reference change values (RCV) and index of individuality (II) of secretoneurin.METHODS: Thirty healthy volunteers were included. Non-fasting samples were obtained between 8-10 am once a week for ten weeks. Secretoneurin was analyzed in duplicate using ELISA. No outliers were present according to Burnett and Reeds' criteria. Simple linear regression did not identify significant trends. Variance homogeneity in the analytical variance and CVI were tested using Cochrane's and Bartlett's tests and four participants were excluded. Calculation...
Source: Clinical Biochemistry - October 8, 2021 Category: Biochemistry Authors: Kristin M Aakre Anett Helleb ø Ottesen Heidi Strand Arne Ludvig Faaren Bashir Alaour Janniche Torsvik Marit S Sylte Michael Marber Geir Christensen Helge R øsjø Torbj ørn Omland Source Type: research

Serum arachidonic acid levels is a predictor of poor functional outcome in acute intracerebral hemorrhage
This study aimed to investigate the relationship between serum levels of PUFAs (including AA) and functional outcomes among intracerebral hemorrhage (ICH) patients.METHODS: From November 2012 to July 2020, ICH patients within 24 h from onset were enrolled. Patients were divided into a good functional outcome group (modified Rankin Scale [mRS] score at 3 months, 0-3) and a poor functional outcome group (mRS score at 3 months, 4-6). We compared baseline variables between groups.RESULTS: Participants comprised 133 patients (mean age, 60 years), with 106 patients (80%) in the good functional outcome group and 27 patients (20%)...
Source: Clinical Biochemistry - October 8, 2021 Category: Biochemistry Authors: Junichiro Takahashi Kenichiro Sakai Takeo Sato Hiroki Takatsu Teppei Komatsu Hidetaka Mitsumura Hidetomo Murakami Yasuyuki Iguchi Source Type: research

Biological variation of secretoneurin; a novel cardiovascular biomarker implicated in arrhythmogenesis
This study reports the within subject variation (CVI), between subject variation (CVG), reference change values (RCV) and index of individuality (II) of secretoneurin.METHODS: Thirty healthy volunteers were included. Non-fasting samples were obtained between 8-10 am once a week for ten weeks. Secretoneurin was analyzed in duplicate using ELISA. No outliers were present according to Burnett and Reeds' criteria. Simple linear regression did not identify significant trends. Variance homogeneity in the analytical variance and CVI were tested using Cochrane's and Bartlett's tests and four participants were excluded. Calculation...
Source: Clinical Biochemistry - October 8, 2021 Category: Biochemistry Authors: Kristin M Aakre Anett Helleb ø Ottesen Heidi Strand Arne Ludvig Faaren Bashir Alaour Janniche Torsvik Marit S Sylte Michael Marber Geir Christensen Helge R øsjø Torbj ørn Omland Source Type: research

Vitamin K intake and health, consideration from the epidemiological studies
J Clin Biochem Nutr. 2021 Sep;69(2):111-121. doi: 10.3164/jcbn.20-64. Epub 2021 Mar 25.ABSTRACTThe most fundamental function of vitamin K is to activate the blood coagulation factors in the liver. Despite the recent recognition of its extra-hepatic actions, the current Dietary Reference Intakes for vitamin K is based on the amount necessary for maintaining the normal blood coagulation in many countries. To define the Dietary Reference Intake for vitamin K, appropriate biomarkers well-reflecting the vitamin K status are essential. Unfortunately, however, no markers are currently available with properties enabling us to prop...
Source: Clinical Biochemistry - October 7, 2021 Category: Biochemistry Authors: Akiko Kuwabara Kazuhiro Uenishi Kiyoshi Tanaka Source Type: research

Cystine supplementation sustains plasma mercaptalbumin levels in rats fed low-protein diets more effectively than methionine
J Clin Biochem Nutr. 2021 Sep;69(2):122-130. doi: 10.3164/jcbn.20-146. Epub 2021 Apr 7.ABSTRACTWe recently reported that dietary cystine maintained plasma mercaptalbumin levels in rats fed low-protein diets. The present study aimed to compare the influence of low-protein diets supplemented with cystine and methionine, which is another sulfur amino acid, on plasma mercaptalbumin levels in rats. Male Sprague-Dawley rats were fed a 20% soy protein isolate diet (control group), 5% soy protein isolate diet (low-protein group) or 5% soy protein isolate diet supplemented with either methionine (low-protein + Met group) or cystine...
Source: Clinical Biochemistry - October 7, 2021 Category: Biochemistry Authors: Yukimi Yano Chihiro Maeda Ichiro Kaneko Yukiko Kobayashi Wataru Aoi Masashi Kuwahata Source Type: research

The cytotoxicity of cyclophosphamide is enhanced in combination with monascus pigment
In this study, we investigated the combination effect of anti-oxidants and anti-cancer drugs. We selected an anti-oxidant as monascus pigment and following four anti-cancer drugs: doxorubicin, tamoxifen, paclitaxicel, and cyclophosphamide. Combination treatment with monascus pigment and cyclophosphamide enhanced the cytotoxicity of cyclophosphamide. Moreover, this combination treatment accelerated apoptosis. The spot on TLC assay board of the monascus pigment and cyclophosphamide mixture is different from the spot of monascus pigment alone and cyclophosphamide alone. The interaction between monascus pigment and cyclo-phosp...
Source: Clinical Biochemistry - October 7, 2021 Category: Biochemistry Authors: Hiromi Kurokawa Atsushi Taninaka Hidemi Shigekawa Hirofumi Matsui Source Type: research

< em > SOD2 < /em > mRNA as a potential biomarker for exercise: interventional and cross-sectional research in healthy subjects
This study was registered with University Hospital Medical Information Network (No: 000038034).PMID:34616105 | PMC:PMC8482385 | DOI:10.3164/jcbn.21-24 (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - October 7, 2021 Category: Biochemistry Authors: Shihona Ogino Noriyoshi Ogino Kotomi Tomizuka Masamitsu Eitoku Yosuke Okada Yoshiya Tanaka Narufumi Suganuma Keiki Ogino Source Type: research

Nobiletin suppresses the development of experimental autoimmune encephalomyelitis mediated by modulation of T helper 17 cell differentiation
In this study, we investigated how nobiletin affects the disease state and immune responses to myelin oligodendrocyte glycoprotein in experimental autoimmune encephalomyelitis mice. Nobiletin was administered orally from 14 days before immunization until the end of the experiment, and clinical scores were determined. The production levels of interleukin-17A and interferon-γ were measured in a culture supernatant of splenocytes stimulated with myelin oligodendrocyte glycoprotein. In addition, flow cytometric analysis was performed to examine the effect of nobiletin on T cell differentiation in vitro. Admin-istration o...
Source: Clinical Biochemistry - October 7, 2021 Category: Biochemistry Authors: Akiko Nakamoto Yuwa Hirabayashi Chieri Anzaki Mariko Nakamoto Emi Shuto Tohru Sakai Source Type: research

< em > Spirulina platensis < /em > and its ingredient biopterin glucoside improved insulin sensitivity in non-alcoholic steatohepatitis model
In this study, we pursued the effect of biopterin glucoside, another bioactive ingredient of Spirulina (Arthrospira) platensis. We found Spirulina (Arthrospira) platensis and biopterin glucoside oral administrations effectively alleviated oxidative stress, inflammation and insulin signal failure, and prevented fibroblast growth factor 21 gene overexpression in non-alcoholic steatohepatitis rat livers. We concluded biopterin glucoside is a major component of Spirulina (Arthrospira) platensis action.PMID:34616107 | PMC:PMC8482380 | DOI:10.3164/jcbn.20-201 (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - October 7, 2021 Category: Biochemistry Authors: Yuri Fujihara Yasumasa Kodo Shin-Ichi Miyoshi Ritsuko Watanabe Hiroshi Toyoda Mitsumasa Mankura Hideaki Kabuto Fusako Takayama Source Type: research

Fermented kimchi rejuvenated precancerous atrophic gastritis via mitigating < em > Helicobacter pylori < /em > -associated endoplasmic reticulum and oxidative stress
J Clin Biochem Nutr. 2021 Sep;69(2):158-170. doi: 10.3164/jcbn.20-180. Epub 2021 Mar 27.ABSTRACTDietary intervention to prevent Helicobacter pylori (H. pylori)-gastric cancer might be ideal by long-term intervention, rejuvenating action, and no risk of bacterial resistance. Stimulated with finding that kimchi prevented H. pylori-gastric cancer, we compared the efficacy of cancer preventive kimchi (cpkimchi) and standard recipe kimchi (skimchi) and the efficacy between fermented kimchi and non-fermented kimchi (kimuchi) in H. pylori-initiated gastric cancer model and explored novel mechanisms hinted from RNAseq transcriptom...
Source: Clinical Biochemistry - October 7, 2021 Category: Biochemistry Authors: Jong Min Park Young Min Han Ji Young Oh Dong Yoon Lee Seung Hye Choi Seong Jin Kim Ki Baik Hahm Source Type: research

Transcriptome profiling implicated in beneficiary actions of kimchi extracts against < em > Helicobacter pylori < /em > infection
J Clin Biochem Nutr. 2021 Sep;69(2):171-187. doi: 10.3164/jcbn.20-116. Epub 2021 Mar 27.ABSTRACTDietary intervention to prevent Helicobacter pylori (H. pylori)-gastric cancer might be ideal because of no risk of bacterial resistance, safety, and rejuvenating action of atrophic gastritis. We have published data about the potential of fermented kimchi as nutritional approach for H. pylori. Hence recent advances in RNAseq analysis lead us to investigate the transcriptome analysis to explain these beneficiary actions of kimchi. gastric cells were infected with either H. pylori or H. pylori plus kimchi. 943 genes were identifie...
Source: Clinical Biochemistry - October 7, 2021 Category: Biochemistry Authors: Jong Min Park Young Min Han Ji Young Oh Dong Yoon Lee Seung Hye Choi Ki Baik Hahm Source Type: research

Therapeutic effects of placenta derived-, umbilical cord derived-, and adipose tissue derived-mesenchymal stem cells in chronic < em > Helicobacter pylori < /em > infection: comparison and novel mechanisms
In this study adopting H. pylori-initiated, high salt diet-promoted gastric carcinogenesis model, we have administered three kinds of MSCs around 15-18 weeks in H. pylori infected C57BL/6 mice and sacrificed at 24 and 48 weeks, respectively, in order to either assess the rejuvenating capability or anti-tumorigenesis. At 24 weeks, MSCs all led to significantly mitigated atrophic gastritis, for which significant inductions of autophagy, preservation of tumor suppressive 15-PGDH, attenuated apoptosis, and efficient efferocytosis was imposed with MSCs administration during atrophic gastritis. At 48 weeks, MSCs administered dur...
Source: Clinical Biochemistry - October 7, 2021 Category: Biochemistry Authors: Jong Min Park Young Min Han Sun Jin Hwang Seong Jin Kim Ki Baik Hahm Source Type: research

The effect of 24-week continuous intake of quercetin-rich onion on age-related cognitive decline in healthy elderly people: a randomized, double-blind, placebo-controlled, parallel-group comparative clinical trial
This study was registered with UMIN (approval number UMIN000036276, 5 April 2019).PMID:34616111 | PMC:PMC8482389 | DOI:10.3164/jcbn.21-17 (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - October 7, 2021 Category: Biochemistry Authors: Jun Nishihira Mie Nishimura Masanori Kurimoto Hiroyo Kagami-Katsuyama Hiroki Hattori Toshiyuki Nakagawa Takato Muro Masuko Kobori Source Type: research

Immunohistochemical differences in gastric mucosal damage between nodular and non-nodular gastritis caused by < em > Helicobacter pylori < /em > infection
In this study, the level of cell damage were analyzed immuno-histochemically to clarify the association between nodular gastritis and undifferentiated gastric cancer. Thirty patients of nodular gastritis were enrolled as the nodular gastritis group. Thirty patients of non-nodular gastritis were enrolled as the control group. They were evaluated according to the updated Sydney system and used for immunohistochemical staining (p53, Ki-67, E-cadherin, and 8-OHdG). The scores based on the updated Sydney system were significantly higher in the nodular group than in the non-nodular group for histologically assessed inflammation ...
Source: Clinical Biochemistry - October 7, 2021 Category: Biochemistry Authors: Kazuhisa Okamoto Masaaki Kodama Kazuhiro Mizukami Tadayoshi Okimoto Hisanori Abe Ryo Ogawa Kensuke Fukuda Osamu Matsunari Yuka Hirashita Yasuhiro Wada Masahide Fukuda Kazunari Murakami Source Type: research

Impact of clinical characteristics of colonic diverticular bleeding in extremely elderly patients treated with direct oral anti-coagulant drugs: a retrospective multi-center study
J Clin Biochem Nutr. 2021 Sep;69(2):222-228. doi: 10.3164/jcbn.20-140. Epub 2021 Apr 3.ABSTRACTSince there were no available data about colonic diverticular bleeding in extremely elderly patients (>80 years old) treated with direct oral anticoagulants (DOACs), we tried to determine clinical characteristics in those with colonic diverticular bleeding taking DOACs and to compare clinical outcomes of those in DOAC-treated to those in warfarin-treated . We enrolled DOAC-treated (n = 20) and warfarin-treated (n = 23) extremely elderly patients with diverticular bleeding diagnosed by colonoscopy. We performed a retrospective ...
Source: Clinical Biochemistry - October 7, 2021 Category: Biochemistry Authors: Kumiko Kirita Yasuhiro Kodaka Yoshiaki Shibata Nobue Ueki Shuhei Agawa Hiroshi Yamawaki Ryota Niikura Nobutake Yamamichi Kentaro Izumi Mariko Hojo Kyohei Maruyama Takatsugu Yamamoto Katya Gudis Masanori Watanabe Mitsuru Kaise Katsuhiko Iwakiri Seiji Futag Source Type: research

Vitamin K intake and health, consideration from the epidemiological studies
J Clin Biochem Nutr. 2021 Sep;69(2):111-121. doi: 10.3164/jcbn.20-64. Epub 2021 Mar 25.ABSTRACTThe most fundamental function of vitamin K is to activate the blood coagulation factors in the liver. Despite the recent recognition of its extra-hepatic actions, the current Dietary Reference Intakes for vitamin K is based on the amount necessary for maintaining the normal blood coagulation in many countries. To define the Dietary Reference Intake for vitamin K, appropriate biomarkers well-reflecting the vitamin K status are essential. Unfortunately, however, no markers are currently available with properties enabling us to prop...
Source: Clinical Biochemistry - October 7, 2021 Category: Biochemistry Authors: Akiko Kuwabara Kazuhiro Uenishi Kiyoshi Tanaka Source Type: research

Cystine supplementation sustains plasma mercaptalbumin levels in rats fed low-protein diets more effectively than methionine
J Clin Biochem Nutr. 2021 Sep;69(2):122-130. doi: 10.3164/jcbn.20-146. Epub 2021 Apr 7.ABSTRACTWe recently reported that dietary cystine maintained plasma mercaptalbumin levels in rats fed low-protein diets. The present study aimed to compare the influence of low-protein diets supplemented with cystine and methionine, which is another sulfur amino acid, on plasma mercaptalbumin levels in rats. Male Sprague-Dawley rats were fed a 20% soy protein isolate diet (control group), 5% soy protein isolate diet (low-protein group) or 5% soy protein isolate diet supplemented with either methionine (low-protein + Met group) or cystine...
Source: Clinical Biochemistry - October 7, 2021 Category: Biochemistry Authors: Yukimi Yano Chihiro Maeda Ichiro Kaneko Yukiko Kobayashi Wataru Aoi Masashi Kuwahata Source Type: research

The cytotoxicity of cyclophosphamide is enhanced in combination with monascus pigment
In this study, we investigated the combination effect of anti-oxidants and anti-cancer drugs. We selected an anti-oxidant as monascus pigment and following four anti-cancer drugs: doxorubicin, tamoxifen, paclitaxicel, and cyclophosphamide. Combination treatment with monascus pigment and cyclophosphamide enhanced the cytotoxicity of cyclophosphamide. Moreover, this combination treatment accelerated apoptosis. The spot on TLC assay board of the monascus pigment and cyclophosphamide mixture is different from the spot of monascus pigment alone and cyclophosphamide alone. The interaction between monascus pigment and cyclo-phosp...
Source: Clinical Biochemistry - October 7, 2021 Category: Biochemistry Authors: Hiromi Kurokawa Atsushi Taninaka Hidemi Shigekawa Hirofumi Matsui Source Type: research

< em > SOD2 < /em > mRNA as a potential biomarker for exercise: interventional and cross-sectional research in healthy subjects
This study was registered with University Hospital Medical Information Network (No: 000038034).PMID:34616105 | PMC:PMC8482385 | DOI:10.3164/jcbn.21-24 (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - October 7, 2021 Category: Biochemistry Authors: Shihona Ogino Noriyoshi Ogino Kotomi Tomizuka Masamitsu Eitoku Yosuke Okada Yoshiya Tanaka Narufumi Suganuma Keiki Ogino Source Type: research

Nobiletin suppresses the development of experimental autoimmune encephalomyelitis mediated by modulation of T helper 17 cell differentiation
In this study, we investigated how nobiletin affects the disease state and immune responses to myelin oligodendrocyte glycoprotein in experimental autoimmune encephalomyelitis mice. Nobiletin was administered orally from 14 days before immunization until the end of the experiment, and clinical scores were determined. The production levels of interleukin-17A and interferon-γ were measured in a culture supernatant of splenocytes stimulated with myelin oligodendrocyte glycoprotein. In addition, flow cytometric analysis was performed to examine the effect of nobiletin on T cell differentiation in vitro. Admin-istration o...
Source: Clinical Biochemistry - October 7, 2021 Category: Biochemistry Authors: Akiko Nakamoto Yuwa Hirabayashi Chieri Anzaki Mariko Nakamoto Emi Shuto Tohru Sakai Source Type: research