Evaluation of three rapid low-resource molecular tests for Nipah virus
Accurate and timely diagnosis of Nipah virus (NiV) requires rapid, inexpensive, and robust diagnostic tests to control spread of disease. Current state of the art technologies are slow and require laboratory infrastructure that may not be available in all endemic settings. Here we report the development and comparison of three rapid NiV molecular diagnostic tests based on reverse transcription recombinase-based isothermal amplification coupled with lateral flow detection. These tests include a simple and fast one-step sample processing step that inactivates the BSL-4 pathogen, enabling safe testing without the need for mul...
Source: Frontiers in Microbiology - February 9, 2023 Category: Microbiology Source Type: research
Recombinant vesicular stomatitis virus–vectored vaccine induces long-lasting immunity against Nipah virus disease
The emergence of the novel henipavirus, Langya virus, received global attention after the virus sickened over three dozen people in China. There is heightened concern that henipaviruses, as respiratory pathogens, could spark another pandemic, most notably the deadly Nipah virus (NiV). NiV causes near-annual outbreaks in Bangladesh and India and induces a highly fatal respiratory disease and encephalitis in humans. No licensed countermeasures against this pathogen exist. An ideal NiV vaccine would confer both fast-acting and long-lived protection. Recently, we reported the generation of a recombinant vesicular stomatitis vi...
Source: Journal of Clinical Investigation - February 2, 2023 Category: Biomedical Science Authors: Courtney Woolsey, Viktoriya Borisevich, Alyssa C. Fears, Krystle N. Agans, Daniel J. Deer, Abhishek N. Prasad, Rachel O’Toole, Stephanie L. Foster, Natalie S. Dobias, Joan B. Geisbert, Karla A. Fenton, Robert W. Cross, Thomas W. Geisbert Source Type: research
Hendra Virus
Hendra virus (HeV) emerged as a zoonotic pathogen in the 1990s, causing low morbidity but high mortality in humans and horses. Pteropid bats are the natural reservoir of HeV and other important zoonotic viruses such as Nipah and Ebola viruses. Equivac HeV, manufactured by Zoetis (Parkville, Victoria, Australia), is the only commercially available vaccine for horses. There is no commercial vaccine for humans. The epidemiology, clinical features, pathology, diagnosis, management, and prevention of HeV will be reviewed. (Source: Veterinary Clinics of North America: Equine Practice)
Source: Veterinary Clinics of North America: Equine Practice - February 1, 2023 Category: Veterinary Research Authors: Xueli Wang, Jessica C. Wise, Allison J. Stewart Source Type: research
Nipah Virus Exposure in Domestic and Peridomestic Animals Living in Human Outbreak Sites, Bangladesh, 2013-2015
Emerg Infect Dis. 2023 Feb;29(2):393-396. doi: 10.3201/eid2902.221379.ABSTRACTSpillovers of Nipah virus (NiV) from Pteropus bats to humans occurs frequently in Bangladesh, but the risk for spillover into other animals is poorly understood. We detected NiV antibodies in cattle, dogs, and cats from 6 sites where spillover human NiV infection cases occurred during 2013-2015.PMID:36692447 | DOI:10.3201/eid2902.221379 (Source: Emerging Infectious Diseases)
Source: Emerging Infectious Diseases - January 24, 2023 Category: Infectious Diseases Authors: Ausraful Islam Deborah L Cannon Mohammed Ziaur Rahman Salah Uddin Khan Jonathan H Epstein Peter Daszak Stephen P Luby Joel M Montgomery John D Klena Emily S Gurley Source Type: research
Nipah Virus Exposure in Domestic and Peridomestic Animals Living in Human Outbreak Sites, Bangladesh, 2013-2015
Emerg Infect Dis. 2023 Feb;29(2):393-396. doi: 10.3201/eid2902.221379.ABSTRACTSpillovers of Nipah virus (NiV) from Pteropus bats to humans occurs frequently in Bangladesh, but the risk for spillover into other animals is poorly understood. We detected NiV antibodies in cattle, dogs, and cats from 6 sites where spillover human NiV infection cases occurred during 2013-2015.PMID:36692447 | DOI:10.3201/eid2902.221379 (Source: Emerging Infectious Diseases)
Source: Emerging Infectious Diseases - January 24, 2023 Category: Infectious Diseases Authors: Ausraful Islam Deborah L Cannon Mohammed Ziaur Rahman Salah Uddin Khan Jonathan H Epstein Peter Daszak Stephen P Luby Joel M Montgomery John D Klena Emily S Gurley Source Type: research
Transmission routes of neuropathogenic pathogens-Possible mechanisms of neuroinvasion
Nervenarzt. 2023 Jan 23. doi: 10.1007/s00115-022-01428-6. Online ahead of print.ABSTRACTDespite natural protective barriers, many human pathogens can penetrate the nervous system. The pathogens have developed sophisticated mechanisms to overturn the privileged immune status of the nervous system. The central nervous system (CNS) has natural barriers and immunological protective mechanisms, such as the blood-brain barrier (BBB) and the blood-cerebrospinal fluid barrier (BCSFB), that prevent the invasion of pathogens. Bacteria enter the CNS, for example, through transcellular penetration, paracellular entry or via infected l...
Source: Der Nervenarzt - January 23, 2023 Category: Neurology Authors: Eva Maria Craemer Source Type: research
Establishment of a neutralization assay for Nipah virus using a high-titer pseudovirus system
CONCLUSIONS: A NiV pseudovirus was successfully generated using HIV vector systems, and was used as a platform for a safe and reliable pseudovirus-based neutralizing assay that can be performed in biosafety level 2 facilities.PMID:36680637 | PMC:PMC9860241 | DOI:10.1007/s10529-023-03351-5 (Source: Biotechnology Letters)
Source: Biotechnology Letters - January 21, 2023 Category: Biotechnology Authors: Xiaoyi Luo Chao Wang Yiman Huang Shanshan Cong Jingjing Tan Wenzhe Hou Fenlian Ma Lishu Zheng Source Type: research
Establishment of a neutralization assay for Nipah virus using a high-titer pseudovirus system
CONCLUSIONS: A NiV pseudovirus was successfully generated using HIV vector systems, and was used as a platform for a safe and reliable pseudovirus-based neutralizing assay that can be performed in biosafety level 2 facilities.PMID:36680637 | DOI:10.1007/s10529-023-03351-5 (Source: Biotechnology Letters)
Source: Biotechnology Letters - January 21, 2023 Category: Biotechnology Authors: Xiaoyi Luo Chao Wang Yiman Huang Shanshan Cong Jingjing Tan Wenzhe Hou Fenlian Ma Lishu Zheng Source Type: research
Molecular Pathogenesis of Nipah Virus
Appl Biochem Biotechnol. 2023 Jan 19. doi: 10.1007/s12010-022-04300-0. Online ahead of print.ABSTRACTViral diseases are causing mayhem throughout the world. One of the zoonotic viruses that have emerged as a potent threat to community health in the past few decades is Nipah virus. Nipah viral sickness is a zoonotic disease whose main carrier is bat. This disease is caused by Nipah virus (NiV). It belongs to the henipavirous group and of the family paramyxoviridae. Predominantly Pteropus spp. is the carrier of this virus. It was first reported from the Kampung Sungai Nipah town of Malaysia in 1998. Human-to-human transmissi...
Source: Applied Biochemistry and Biotechnology - January 19, 2023 Category: Biochemistry Authors: Pratik Talukdar Debankita Dutta Elija Ghosh Indrani Bose Sourish Bhattacharjee Source Type: research
Molecular Pathogenesis of Nipah Virus
Appl Biochem Biotechnol. 2023 Jan 19. doi: 10.1007/s12010-022-04300-0. Online ahead of print.ABSTRACTViral diseases are causing mayhem throughout the world. One of the zoonotic viruses that have emerged as a potent threat to community health in the past few decades is Nipah virus. Nipah viral sickness is a zoonotic disease whose main carrier is bat. This disease is caused by Nipah virus (NiV). It belongs to the henipavirous group and of the family paramyxoviridae. Predominantly Pteropus spp. is the carrier of this virus. It was first reported from the Kampung Sungai Nipah town of Malaysia in 1998. Human-to-human transmissi...
Source: Applied Biochemistry and Biotechnology - January 19, 2023 Category: Biochemistry Authors: Pratik Talukdar Debankita Dutta Elija Ghosh Indrani Bose Sourish Bhattacharjee Source Type: research
Nipah Virus Exposure in Domestic and Peridomestic Animals Living in Human Outbreak Sites, Bangladesh, 2013 –2015
A. Islam et al. (Source: Emerging Infectious Diseases Journal)
Source: Emerging Infectious Diseases Journal - January 11, 2023 Category: Infectious Diseases Source Type: research
Risk of Viral Infectious Diseases from Live Bats, Primates, Rodents and Carnivores for Sale in Indonesian Wildlife Markets
This study highlights the need for better surveillance and sanitary conditions to avoid the negative health impacts of unregulated wildlife markets.PMID:36560762 | PMC:PMC9786693 | DOI:10.3390/v14122756 (Source: Herpes)
Source: Herpes - December 23, 2022 Category: Infectious Diseases Authors: Thais Q Morcatty Paula E R Pereyra Ahmad Ardiansyah Muhammad Ali Imron Katherine Hedger Marco Campera K Anne-Isola Nekaris Vincent Nijman Source Type: research
Risk of Viral Infectious Diseases from Live Bats, Primates, Rodents and Carnivores for Sale in Indonesian Wildlife Markets
This study highlights the need for better surveillance and sanitary conditions to avoid the negative health impacts of unregulated wildlife markets.PMID:36560762 | PMC:PMC9786693 | DOI:10.3390/v14122756 (Source: Herpes)
Source: Herpes - December 23, 2022 Category: Infectious Diseases Authors: Thais Q Morcatty Paula E R Pereyra Ahmad Ardiansyah Muhammad Ali Imron Katherine Hedger Marco Campera K Anne-Isola Nekaris Vincent Nijman Source Type: research
Development of Nipah virus-specific IgM & amp; IgG ELISA for screening human serum samples
Indian J Med Res. 2022 Dec 13. doi: 10.4103/ijmr.ijmr_2737_21. Online ahead of print.NO ABSTRACTPMID:36510890 | DOI:10.4103/ijmr.ijmr_2737_21 (Source: The Indian Journal of Medical Research)
Source: The Indian Journal of Medical Research - December 13, 2022 Category: Biomedical Science Authors: Anita M Shete Rajlaxmi Jain Sreelekshmy Mohandas Prachi Pardeshi Pragya D Yadav Nivedita Gupta Devendra Mourya Source Type: research
Development of Nipah virus-specific IgM & amp; IgG ELISA for screening human serum samples
Indian J Med Res. 2022 Dec 13. doi: 10.4103/ijmr.ijmr_2737_21. Online ahead of print.NO ABSTRACTPMID:36510890 | DOI:10.4103/ijmr.ijmr_2737_21 (Source: Indian J Med Res)
Source: Indian J Med Res - December 13, 2022 Category: Research Authors: Anita M Shete Rajlaxmi Jain Sreelekshmy Mohandas Prachi Pardeshi Pragya D Yadav Nivedita Gupta Devendra Mourya Source Type: research
