Rapid detection of Nipah virus using the one-pot RPA-CRISPR/Cas13a assay
In this study, we developed an optimized one-pot assay using recombinase polymerase amplification (RPA) coupled to CRISPR/Cas13a for the molecular detection of NiV. The one-pot RPA-CRISPR/Cas13a assay for NiV detection was specific and did not cross-react against other selected (re)-emerging pathogens. The sensitivity of the one-pot RPA-CRISPR/Cas13a assay for NiV detection can detect as little as 103 cp/μL of total synthetic NiV cDNA. The assay was then validated with simulated clinical samples. The results for the one-pot RPA-CRISPR/Cas13a assay could be visualized with either fluorescence or lateral flow strips for con...
Source: Virus Research - May 13, 2023 Category: Virology Authors: Jing Miao Lulu Zuo Dongmei He Zhixin Fang Nicolas Berthet Chao Yu Gary Wong Source Type: research
Rapid detection of Nipah virus using the one-pot RPA-CRISPR/Cas13a assay
In this study, we developed an optimized one-pot assay using recombinase polymerase amplification (RPA) coupled to CRISPR/Cas13a for the molecular detection of NiV. The one-pot RPA-CRISPR/Cas13a assay for NiV detection was specific and did not cross-react against other selected (re)-emerging pathogens. The sensitivity of the one-pot RPA-CRISPR/Cas13a assay for NiV detection can detect as little as 103 cp/μL of total synthetic NiV cDNA. The assay was then validated with simulated clinical samples. The results for the one-pot RPA-CRISPR/Cas13a assay could be visualized with either fluorescence or lateral flow strips for con...
Source: Virus Research - May 13, 2023 Category: Virology Authors: Jing Miao Lulu Zuo Dongmei He Zhixin Fang Nicolas Berthet Chao Yu Gary Wong Source Type: research
Rapid detection of Nipah virus using the one-pot RPA-CRISPR/Cas13a assay
In this study, we developed an optimized one-pot assay using recombinase polymerase amplification (RPA) coupled to CRISPR/Cas13a for the molecular detection of NiV. The one-pot RPA-CRISPR/Cas13a assay for NiV detection was specific and did not cross-react against other selected (re)-emerging pathogens. The sensitivity of the one-pot RPA-CRISPR/Cas13a assay for NiV detection can detect as little as 103 cp/μL of total synthetic NiV cDNA. The assay was then validated with simulated clinical samples. The results for the one-pot RPA-CRISPR/Cas13a assay could be visualized with either fluorescence or lateral flow strips for con...
Source: Virus Research - May 13, 2023 Category: Virology Authors: Jing Miao Lulu Zuo Dongmei He Zhixin Fang Nicolas Berthet Chao Yu Gary Wong Source Type: research
Viruses, Vol. 15, Pages 1077: A Recombinant Chimeric Cedar Virus-Based Surrogate Neutralization Assay Platform for Pathogenic Henipaviruses
t
Christopher C. Broder
The henipaviruses, Nipah virus (NiV), and Hendra virus (HeV) can cause fatal diseases in humans and animals, whereas Cedar virus is a nonpathogenic henipavirus. Here, using a recombinant Cedar virus (rCedV) reverse genetics platform, the fusion (F) and attachment (G) glycoprotein genes of rCedV were replaced with those of NiV-Bangladesh (NiV-B) or HeV, generating replication-competent chimeric viruses (rCedV-NiV-B and rCedV-HeV), both with and without green fluorescent protein (GFP) or luciferase protein genes. The rCedV chimeras induced a Type I interferon response and utilized only ephrin-B2...
Source: Viruses - April 28, 2023 Category: Virology Authors: Moushimi Amaya Randy Yin Lianying Yan Viktoriya Borisevich Bishwo N. Adhikari Andrew Bennett Francisco Malagon Regina Z. Cer Kimberly A. Bishop-Lilly Antony S. Dimitrov Robert W. Cross Thomas W. Geisbert Christopher C. Broder Tags: Article Source Type: research
Immune correlates of protection for SARS-CoV-2, Ebola and Nipah virus infection
Correlates of protection (CoP) are biological parameters that predict a certain level of protection against an infectious disease. Well-established correlates of protection facilitate the development and licensing of vaccines by assessing protective efficacy without the need to expose clinical trial participants to the infectious agent against which the vaccine aims to protect. Despite the fact that viruses have many features in common, correlates of protection can vary considerably amongst the same virus family and even amongst a same virus depending on the infection phase that is under consideration. Moreover, the comple...
Source: Frontiers in Immunology - April 17, 2023 Category: Allergy & Immunology Source Type: research
Viruses, Vol. 15, Pages 961: Analysis of Nipah Virus Replication and Host Proteome Response Patterns in Differentiated Porcine Airway Epithelial Cells Cultured at the Air & ndash;Liquid Interface
Viruses, Vol. 15, Pages 961: Analysis of Nipah Virus Replication and Host Proteome Response Patterns in Differentiated Porcine Airway Epithelial Cells Cultured at the Air–Liquid Interface
Viruses doi: 10.3390/v15040961
Authors:
Martin Müller
Kerstin Fischer
Elisabeth Woehnke
Luca M. Zaeck
Christoph Prönnecke
Michael R. Knittler
Axel Karger
Sandra Diederich
Stefan Finke
Respiratory tract epithelium infection plays a primary role in Nipah virus (NiV) pathogenesis and transmission. Knowledge about infection dynamics and host responses to NiV infection in respiratory tract epithelia is scarc...
Source: Viruses - April 13, 2023 Category: Virology Authors: Martin M üller Kerstin Fischer Elisabeth Woehnke Luca M. Zaeck Christoph Pr önnecke Michael R. Knittler Axel Karger Sandra Diederich Stefan Finke Tags: Article Source Type: research
The pathogenesis of zoonotic viral infections: Lessons learned by studying reservoir hosts
Zoonotic viral infections that cause severe disease or even death in some people may be asymptomatic or mild in reservoir hosts. Comparison of the pathogenesis of these two host categories may potentially explain the difference in disease. However, infections in reservoir hosts are often neglected. Therefore, we compared the pathogenesis of rabies virus, macacine alphaherpesvirus, West Nile virus, Puumala orthohantavirus, monkeypox virus, Lassa mammarenavirus, H5N1 highly pathogenic avian influenza, Marburg virus, Nipah virus, Middle East respiratory syndrome, and simian/human immunodeficiency viruses in both humans and re...
Source: Frontiers in Microbiology - March 28, 2023 Category: Microbiology Source Type: research
Pseudotyped Virus for Henipavirus
Adv Exp Med Biol. 2023;1407:175-190. doi: 10.1007/978-981-99-0113-5_9.ABSTRACTThe genus Henipavirus (HNV) includes two virulent infectious viruses, Nipah virus (NiV) and Hendra virus (HeV), which are the focus of considerable public health research efforts and have been classified as priority infectious diseases by the World Health Organization. Both viruses are high risk and should be handled in biosafety level 4 laboratories. Pseudotyped viruses containing the envelope proteins of HNV viruses have the same envelope protein structure as the authentic viruses; thus, they can mimic the receptor-binding and membrane fusion p...
Source: Advances in Experimental Medicine and Biology - March 15, 2023 Category: Research Authors: Tao Li Ziteng Liang Weijin Huang Youchun Wang Source Type: research
Computational identification of drug-like marine natural products as potential RNA polymerase inhibitors against Nipah virus
Comput Biol Chem. 2023 Mar 7;104:107850. doi: 10.1016/j.compbiolchem.2023.107850. Online ahead of print.ABSTRACTNipah virus (NiV) has been an alarming threat to human populations in southern Asia for more than a decade. It is one of the most deadly viruses in the Mononegavirales order. Despite its high mortality rate and virulence, no chemotherapeutic agent or vaccine is publicly available. Hence, this work was conducted to computationally screen marine natural products database for drug-like potential inhibitors for the viral RNA-dependent RNA polymerase (RdRp). The structural model was subjected to molecular dynamics (MD...
Source: Computational Biology and Chemistry - March 12, 2023 Category: Bioinformatics Authors: Jameel M Abduljalil Abdo A Elfiky El-Sayed T A Sayed Maha M AlKhazindar Source Type: research
Computational identification of drug-like marine natural products as potential RNA polymerase inhibitors against Nipah virus
Comput Biol Chem. 2023 Mar 7;104:107850. doi: 10.1016/j.compbiolchem.2023.107850. Online ahead of print.ABSTRACTNipah virus (NiV) has been an alarming threat to human populations in southern Asia for more than a decade. It is one of the most deadly viruses in the Mononegavirales order. Despite its high mortality rate and virulence, no chemotherapeutic agent or vaccine is publicly available. Hence, this work was conducted to computationally screen marine natural products database for drug-like potential inhibitors for the viral RNA-dependent RNA polymerase (RdRp). The structural model was subjected to molecular dynamics (MD...
Source: Computational Biology and Chemistry - March 12, 2023 Category: Bioinformatics Authors: Jameel M Abduljalil Abdo A Elfiky El-Sayed T A Sayed Maha M AlKhazindar Source Type: research
Computational identification of drug-like marine natural products as potential RNA polymerase inhibitors against Nipah virus
Comput Biol Chem. 2023 Mar 7;104:107850. doi: 10.1016/j.compbiolchem.2023.107850. Online ahead of print.ABSTRACTNipah virus (NiV) has been an alarming threat to human populations in southern Asia for more than a decade. It is one of the most deadly viruses in the Mononegavirales order. Despite its high mortality rate and virulence, no chemotherapeutic agent or vaccine is publicly available. Hence, this work was conducted to computationally screen marine natural products database for drug-like potential inhibitors for the viral RNA-dependent RNA polymerase (RdRp). The structural model was subjected to molecular dynamics (MD...
Source: Computational Biology and Chemistry - March 12, 2023 Category: Bioinformatics Authors: Jameel M Abduljalil Abdo A Elfiky El-Sayed T A Sayed Maha M AlKhazindar Source Type: research
Computational identification of drug-like marine natural products as potential RNA polymerase inhibitors against Nipah virus
Comput Biol Chem. 2023 Mar 7;104:107850. doi: 10.1016/j.compbiolchem.2023.107850. Online ahead of print.ABSTRACTNipah virus (NiV) has been an alarming threat to human populations in southern Asia for more than a decade. It is one of the most deadly viruses in the Mononegavirales order. Despite its high mortality rate and virulence, no chemotherapeutic agent or vaccine is publicly available. Hence, this work was conducted to computationally screen marine natural products database for drug-like potential inhibitors for the viral RNA-dependent RNA polymerase (RdRp). The structural model was subjected to molecular dynamics (MD...
Source: Computational Biology and Chemistry - March 12, 2023 Category: Bioinformatics Authors: Jameel M Abduljalil Abdo A Elfiky El-Sayed T A Sayed Maha M AlKhazindar Source Type: research
Viruses, Vol. 15, Pages 599: Host & ndash;Pathogen Interactions Influencing Zoonotic Spillover Potential and Transmission in Humans
Viruses, Vol. 15, Pages 599: Host–Pathogen Interactions Influencing Zoonotic Spillover Potential and Transmission in Humans
Viruses doi: 10.3390/v15030599
Authors:
Beatriz Escudero-Pérez
Alexandre Lalande
Cyrille Mathieu
Philip Lawrence
Emerging infectious diseases of zoonotic origin are an ever-increasing public health risk and economic burden. The factors that determine if and when an animal virus is able to spill over into the human population with sufficient success to achieve ongoing transmission in humans are complex and dynamic. We are currently unable to fully predict which pathogens may ap...
Source: Viruses - February 22, 2023 Category: Virology Authors: Beatriz Escudero-P érez Alexandre Lalande Cyrille Mathieu Philip Lawrence Tags: Review Source Type: research
Viruses, Vol. 15, Pages 554: Nipah Virus Impairs Autocrine IFN Signaling by Sequestering STAT1 and STAT2 into Inclusion Bodies
ner
Nipah virus (NiV) is an emerging zoonotic paramyxovirus that causes fatal infections in humans. As with most disease-causing viruses, the pathogenic potential of NiV is linked to its ability to block antiviral responses, e.g., by antagonizing IFN signaling through blocking STAT proteins. One of the STAT1/2-binding proteins of NiV is the phosphoprotein (P), but its functional role in IFN antagonism in a full viral context is not well defined. As NiV P is required for genome replication and specifically accumulates in cytosolic inclusion bodies (IBs) of infected cells, we hypothesized that this compartmentalization m...
Source: Viruses - February 17, 2023 Category: Virology Authors: Nico Becker Andrea Maisner Tags: Article Source Type: research
Correction: Prioritizing surveillance of Nipah virus in India
by Raina K. Plowright, Daniel J. Becker, Daniel E. Crowley, Alex D. Washburne, Tao Huang, P. O. Nameer, Emily S. Gurley, Barbara A. Han (Source: PLoS Neglected Tropical Diseases)
Source: PLoS Neglected Tropical Diseases - February 10, 2023 Category: Tropical Medicine Authors: Raina K. Plowright Source Type: research
