Rapid detection of Nipah virus using the one-pot RPA-CRISPR/Cas13a assay
In this study, we developed an optimized one-pot assay using recombinase polymerase amplification (RPA) coupled to CRISPR/Cas13a for the molecular detection of NiV. The one-pot RPA-CRISPR/Cas13a assay for NiV detection was specific and did not cross-react against other selected (re)-emerging pathogens. The sensitivity of the one-pot RPA-CRISPR/Cas13a assay for NiV detection can detect as little as 103 cp/μL of total synthetic NiV cDNA. The assay was then validated with simulated clinical samples. The results for the one-pot RPA-CRISPR/Cas13a assay could be visualized with either fluorescence or lateral flow strips for convenient clinical or field diagnostics, providing a useful supplement to the gold-standard qRT-PCR assay for detecting NiV detections.PMID:37178792 | DOI:10.1016/j.virusres.2023.199130
Source: Virus Research - Category: Virology Authors: Jing Miao Lulu Zuo Dongmei He Zhixin Fang Nicolas Berthet Chao Yu Gary Wong Source Type: research
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