IFITM3 promotes NiV envelope protein-mediated entry into MDCK cells and interacts with the fusion subunit of the F protein
Int J Biochem Cell Biol. 2022 Oct 28;153:106325. doi: 10.1016/j.biocel.2022.106325. Online ahead of print.ABSTRACTIFITM proteins are a host restriction factor with broad-spectrum antiviral activity, but the role in the paramyxovirus entry remains unclear. Nipah virus (NiV) is a zoonotic virus of the paramyxoviridae with extremely high lethality. Here, we assessed the role of IFITM3 on NiV G and F glycoprotein-mediated virus entry. Using NiV pseudovirus bearing NiV G and F proteins to infect IFITM3-induced MDCK cells, we found that overexpression of IFITM3 promotes NiV G and F proteins-mediated virus entry. Mechanistically,...
Source: The International Journal of Biochemistry and Cell Biology - November 4, 2022 Category: Biochemistry Authors: Wang Xu Shou-Wen Du Le-Tian Li Xiao-Shuang Shi Jia-Min Wang Ti-Yuan Li Ning-Yi Jin Chang Li Source Type: research
IFITM3 promotes NiV envelope protein-mediated entry into MDCK cells and interacts with the fusion subunit of the F protein
Int J Biochem Cell Biol. 2022 Oct 28;153:106325. doi: 10.1016/j.biocel.2022.106325. Online ahead of print.ABSTRACTIFITM proteins are a host restriction factor with broad-spectrum antiviral activity, but the role in the paramyxovirus entry remains unclear. Nipah virus (NiV) is a zoonotic virus of the paramyxoviridae with extremely high lethality. Here, we assessed the role of IFITM3 on NiV G and F glycoprotein-mediated virus entry. Using NiV pseudovirus bearing NiV G and F proteins to infect IFITM3-induced MDCK cells, we found that overexpression of IFITM3 promotes NiV G and F proteins-mediated virus entry. Mechanistically,...
Source: The International Journal of Biochemistry and Cell Biology - November 4, 2022 Category: Biochemistry Authors: Wang Xu Shou-Wen Du Le-Tian Li Xiao-Shuang Shi Jia-Min Wang Ti-Yuan Li Ning-Yi Jin Chang Li Source Type: research
A short communication of Nipah virus outbreak in India: An urgent rising concern
Ann Med Surg (Lond). 2022 Sep 8;82:104599. doi: 10.1016/j.amsu.2022.104599. eCollection 2022 Oct.ABSTRACTIn the past two decades, countries like Malaysia, Singapore, Bangladesh, and India have recorded several cases of Nipah virus (NiV) infection. Following the 2018 NiV outbreak in the Kozhikode district of Kerala, India that claimed 17 lives, there has been a recent re-emergence of the virus in the same district, causing the recently reported death of a 12-year-old boy. Accordingly, population panic has heightened as inhabitants of these areas try to together combat the existing COVID-19 pandemic alongside the emerging Ni...
Source: Annals of Medicine - October 21, 2022 Category: Internal Medicine Authors: Olivier Uwishema Jack Wellington Christin Berjaoui Kamsi Olivia Muoka Chinyere Vivian Patrick Onyeaka Helen Onyeaka Source Type: research
Viruses, Vol. 14, Pages 2167: First Genomic Evidence of a Henipa-like Virus in Brazil
Vasconcelos
Cruz
The viral genus Henipavirus includes two highly virulent zoonotic viruses of serious public health concern. Hendra henipavirus and Nipah henipavirus outbreaks are restricted to Australia and Southeast Asia, respectively. The Henipavirus genus comprises mostly bat-borne viruses, but exceptions have already been described as novel viruses with rodents and shrews as reservoir animals. In the Americas, scarce evidence supports the circulation of these viruses. In this communication, we report a novel henipa-like virus from opossums (Marmosa demerarae) from a forest fragment area in the Peixe-Boi municip...
Source: Viruses - September 30, 2022 Category: Virology Authors: Hern ández da Paz Silva Silva Barros Nunes Casseb Medeiros Vasconcelos Cruz Tags: Communication Source Type: research
Viruses, Vol. 14, Pages 2057: Aerosol Survival, Disinfection and Formalin Inactivation of Nipah Virus
Lever
Nipah virus is a relatively newly discovered emerging virus on the WHO list of priority pathogens which has the potential to cause outbreaks with high fatality rates. Whilst progress is being made in the development of animal models for evaluating vaccines and therapies, some of the more fundamental data on Nipah virus are lacking. We performed studies to generate novel information on the aerosol survival of Nipah virus and to look at the efficacy of two common disinfectants. We also performed studies to evaluate the inactivation of Nipah virus by using neutral buffered formalin. Nipah virus was relatively st...
Source: Viruses - September 16, 2022 Category: Virology Authors: Smither Eastaugh O ’Brien Phelps Lever Tags: Article Source Type: research
A platform technology for generating subunit vaccines against diverse viral pathogens
The COVID-19 pandemic response has shown how vaccine platform technologies can be used to rapidly and effectively counteract a novel emerging infectious disease. The speed of development for mRNA and vector-based vaccines outpaced those of subunit vaccines, however, subunit vaccines can offer advantages in terms of safety and stability. Here we describe a subunit vaccine platform technology, the molecular clamp, in application to four viruses from divergent taxonomic families: Middle Eastern respiratory syndrome coronavirus (MERS-CoV), Ebola virus (EBOV), Lassa virus (LASV) and Nipah virus (NiV). The clamp streamlines subu...
Source: Frontiers in Immunology - August 18, 2022 Category: Allergy & Immunology Source Type: research
New ‘Langya’ virus identified in China: what scientists know so far
Nature, Published online: 11 August 2022; doi:10.1038/d41586-022-02175-zThe henipavirus can cause respiratory symptoms and is related to Nipah and Hendra viruses, but cannot spread easily in people. (Source: Nature AOP)
Source: Nature AOP - August 11, 2022 Category: Research Authors: Smriti Mallapaty Source Type: research
Viruses, Vol. 14, Pages 1657: Detection of Nipah and Hendra Viruses Using Recombinant Human Ephrin B2 Capture Virus in Immunoassays
In this study, the henipavirus receptor ephrin B2 was examined to determine whether it could be used as a universal ligand for henipavirus detection in immunoassays. Enzyme-linked immunosorbent assays (ELISAs) were developed using recombinant ephrin B2 as the capture ligand and two monoclonal antibodies (mAbs) as detection reagents. Using mAb F27NiV-34, which cross-reacts with NiV and HeV, we were able to detect NiV and HeV, while mAb F20NiV-65 was used to detect NiV. Therefore, using these two ELISAs, we were able to differentiate between NiV and HeV. Furthermore, we developed a rapid lateral flow strip test for NiV detec...
Source: Viruses - July 28, 2022 Category: Virology Authors: Ming Yang Wenjun Zhu Thang Truong Bradley Pickering Shawn Babiuk Darwyn Kobasa Logan Banadyga Tags: Article Source Type: research
Viruses, Vol. 14, Pages 1654: Characterization of Pipistrellus pygmaeus Bat Virome from Sweden
This study expands our knowledge on the bat population virome and improves our understanding of the evolution and transmission of viruses among bats and to other species. (Source: Viruses)
Source: Viruses - July 28, 2022 Category: Virology Authors: Harindranath Cholleti Johnny de Jong Anne-Lie Blomstr öm Mikael Berg Tags: Article Source Type: research
Corrigendum to "Newcastle disease virus vectored Nipah encephalitis vaccines induce B and T cell responses in mice and long-lasting neutralizing antibodies in pigs" [Virology 432 (2012) 327-335]
Virology. 2022 Aug;573:176-177. doi: 10.1016/j.virol.2022.07.001. Epub 2022 Jul 13.NO ABSTRACTPMID:35839550 | DOI:10.1016/j.virol.2022.07.001 (Source: Virology)
Source: Virology - July 15, 2022 Category: Virology Authors: Dongni Kong Zhiyuan Wen Hua Su Jinying Ge Weiye Chen Xijun Wang Chao Wu Chinglai Yang Hualan Chen Zhigao Bu Source Type: research
Corrigendum to "Newcastle disease virus vectored Nipah encephalitis vaccines induce B and T cell responses in mice and long-lasting neutralizing antibodies in pigs" [Virology 432 (2012) 327-335]
Virology. 2022 Aug;573:176-177. doi: 10.1016/j.virol.2022.07.001. Epub 2022 Jul 13.NO ABSTRACTPMID:35839550 | DOI:10.1016/j.virol.2022.07.001 (Source: Virology)
Source: Virology - July 15, 2022 Category: Virology Authors: Dongni Kong Zhiyuan Wen Hua Su Jinying Ge Weiye Chen Xijun Wang Chao Wu Chinglai Yang Hualan Chen Zhigao Bu Source Type: research
Corrigendum to "Newcastle disease virus vectored Nipah encephalitis vaccines induce B and T cell responses in mice and long-lasting neutralizing antibodies in pigs" [Virology 432 (2012) 327-335]
Virology. 2022 Jul 12;573:176-177. doi: 10.1016/j.virol.2022.07.001. Online ahead of print.NO ABSTRACTPMID:35839550 | DOI:10.1016/j.virol.2022.07.001 (Source: Virology)
Source: Virology - July 15, 2022 Category: Virology Authors: Dongni Kong Zhiyuan Wen Hua Su Jinying Ge Weiye Chen Xijun Wang Chao Wu Chinglai Yang Hualan Chen Zhigao Bu Source Type: research
Corrigendum to "Newcastle disease virus vectored Nipah encephalitis vaccines induce B and T cell responses in mice and long-lasting neutralizing antibodies in pigs" [Virology 432 (2012) 327-335]
Virology. 2022 Jul 12;573:176-177. doi: 10.1016/j.virol.2022.07.001. Online ahead of print.NO ABSTRACTPMID:35839550 | DOI:10.1016/j.virol.2022.07.001 (Source: Virology)
Source: Virology - July 15, 2022 Category: Virology Authors: Dongni Kong Zhiyuan Wen Hua Su Jinying Ge Weiye Chen Xijun Wang Chao Wu Chinglai Yang Hualan Chen Zhigao Bu Source Type: research
Viruses, Vol. 14, Pages 1523: Nipah Virus Infection Generates Ordered Structures in Cellulo
Beatriz Escudero-Pérez
Nipah virus (NiV) is a zoonotic paramyxovirus with a fatality rate of up to 92% in humans. While several pathogenic mechanisms used by NiV to counteract host immune defense responses have been described, all of the processes that take place in cells during infection are not fully characterized. Here, we describe the formation of ordered intracellular structures during NiV infection. We observed that these structures are formed specifically during NiV infection, but not with other viruses from the same Mononegavirales order (namely Ebola virus) or from other orders such as Bunyavirales (Jun&a...
Source: Viruses - July 12, 2022 Category: Virology Authors: Cecilia Alejandra V ázquez Lina Widerspick Roland Thuenauer Carola Schneider Rudolph Reimer Pedro Neira Catherine Olal Michelle Heung Linda Niemetz Philip Lawrence Indre Kucinskaite-Kodze Lars Redecke Beatriz Escudero-P érez Tags: Communication Source Type: research
